Restriction map for
pF1KSDA1279
(Circular) (Six-base) MAPSORT of: pF1KSDA1279.seq Check: 315 from: 1 to: 5000
pF1KSDA1279 5000 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 776 1502 2038 776
Size: 726 536 3738
Fragments arranged by size:
3738 726 536
Acc65I G'GTAC_C
Cuts at: 506 2016 506
Size: 1510 3490
AclI AA'CG_TT
Cuts at: 134 4840 134
Size: 4706 294
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 713 1025 1031 1687 2825 3257 4004 4272 713
Size: 312 6 656 1138 432 747 268 1441
Fragments arranged by size:
1441 1138 747 656 432 312 268 6
AflIII A'CryG_T
Cuts at: 2352 3456 4433 2352
Size: 1104 977 2919
Fragments arranged by size:
2919 1104 977
AgeI A'CCGG_T
Cuts at: 1225 3388 1225
Size: 2163 2837
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 168 168
Size: 5000
AlwNI CAG_nnn'CTG
Cuts at: 786 938 2305 3872 786
Size: 152 1367 1567 1914
Fragments arranged by size:
1914 1567 1367 152
ApaI G_GGCC'C
Cuts at: 383 383
Size: 5000
ApaLI G'TGCA_C
Cuts at: 3770 3770
Size: 5000
ApoI r'AATT_y
Cuts at: 160 1827 1855 2004 4353 4609 160
Size: 1667 28 149 2349 256 551
Fragments arranged by size:
2349 1667 551 256 149 28
AseI AT'TA_AT
Cuts at: 20 20
Size: 5000
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5000
AvaI C'yCGr_G
Cuts at: 242 324 373 427 2020 242
Size: 82 49 54 1593 3222
Fragments arranged by size:
3222 1593 82 54 49
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 546 579 546
Size: 33 4967
BamHI G'GATC_C
Cuts at: 1614 2025 1614
Size: 411 4589
BanI G'GyrC_C
Cuts at: 328 506 2016 2430 2678 2713 328
Size: 178 1510 414 248 35 2615
Fragments arranged by size:
2615 1510 414 248 178 35
BanII G_rGCy'C
Cuts at: 374 383 1493 2014 3044 374
Size: 9 1110 521 1030 2330
Fragments arranged by size:
2330 1110 1030 521 9
BbeI G_GCGC'C
Cuts at: 2434 2682 2434
Size: 248 4752
BbsI GAAGACnn'nnnn_
Cuts at: 1001 1933 1001
Size: 932 4068
BbvCI CC'TCA_GC
Cuts at: 359 359
Size: 5000
BciVI GTATCCnnnnn_n'
Cuts at: 2898 3659 5000 2898
Size: 761 1341 2898
Fragments arranged by size:
2898 1341 761
BclI T'GATC_A
Cuts at: 2520 2520
Size: 5000
BfrBI ATG'CAT
Cuts at: 1464 1464
Size: 5000
BglI GCCn_nnn'nGGC
Cuts at: 378 378
Size: 5000
BglII A'GATC_T
Cuts at: 980 2515 4552 980
Size: 1535 2037 1428
Fragments arranged by size:
2037 1535 1428
BlpI GC'TnA_GC
Cuts at: 2112 2112
Size: 5000
Bme1580I G_kGCm'C
Cuts at: 383 2625 2718 3774 383
Size: 2242 93 1056 1609
Fragments arranged by size:
2242 1609 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2212 2293 2628 4772 2212
Size: 81 335 2144 2440
Fragments arranged by size:
2440 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 680 3367 680
Size: 2687 2313
Bpu10I CC'TnA_GC
Cuts at: 359 1609 1721 359
Size: 1250 112 3638
Fragments arranged by size:
3638 1250 112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 755 862 1296 2176 3055 3547 3845 755
Size: 107 434 880 879 492 298 1910
Fragments arranged by size:
1910 880 879 492 434 298 107
BsaI GGTCTCn'nnnn_
Cuts at: 1272 1694 4704 1272
Size: 422 3010 1568
Fragments arranged by size:
3010 1568 422
BsaAI yAC'GTr
Cuts at: 1992 2353 1992
Size: 361 4639
BsaBI GATnn'nnATC
Cuts at: 6 2514 4551 6
Size: 2508 2037 455
Fragments arranged by size:
2508 2037 455
BsaHI Gr'CG_yC
Cuts at: 2431 2679 3381 4877 2431
Size: 248 702 1496 2554
Fragments arranged by size:
2554 1496 702 248
BsaWI w'CCGG_w
Cuts at: 207 1225 2189 2710 3388 3662 3809 4220 207
Size: 1018 964 521 678 274 147 411 987
Fragments arranged by size:
1018 987 964 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 442 472 1438 1468 442
Size: 30 966 30 3974
Fragments arranged by size:
3974 966 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 466 484 653 1201 466
Size: 18 169 548 4265
Fragments arranged by size:
4265 548 169 18
BseYI C'CCAG_C
Cuts at: 389 2425 3183 3760 4256 389
Size: 2036 758 577 496 1133
Fragments arranged by size:
2036 1133 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 581 1234 581
Size: 653 4347
BsiEI CG_ry'CG
Cuts at: 104 2542 2588 3796 104
Size: 2438 46 1208 1308
Fragments arranged by size:
2438 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 476 848 1410 2014 2792 2982 3774 476
Size: 372 562 604 778 190 792 1702
Fragments arranged by size:
1702 792 778 604 562 372 190
BsmI GAATG_Cn'
Cuts at: 1101 1196 1426 1101
Size: 95 230 4675
Fragments arranged by size:
4675 230 95
BsmBI CGTCTCn'nnnn_
Cuts at: 1443 1786 1443
Size: 343 4657
Bsp1286I G_dGCh'C
Cuts at: 374 383 476 848 1410 1493 2014 2625 2718
Size: 9 93 372 562 83 521 611 93
Cuts at: 2718 2792 2982 3044 3774 374
Size: 74 190 62 730 1600
Fragments arranged by size:
1600 730 611 562 521 372 190 93
93 83 74 62 9
BspEI T'CCGG_A
Cuts at: 207 4220 207
Size: 4013 987
BspHI T'CATG_A
Cuts at: 4176 4176
Size: 5000
BspMI ACCTGCnnnn'nnnn_
Cuts at: 494 2050 2264 2566 2947 494
Size: 1556 214 302 381 2547
Fragments arranged by size:
2547 1556 381 302 214
BsrBI CCG'CTC
Cuts at: 50 303 397 3292 3346 4830 4995 50
Size: 253 94 2895 54 1484 165 55
Fragments arranged by size:
2895 1484 253 165 94 55 54
BsrDI GCAATG_nn'
Cuts at: 1595 2912 1595
Size: 1317 3683
BsrFI r'CCGG_y
Cuts at: 331 354 509 1225 2998 3179 3388 331
Size: 23 155 716 1773 181 209 1943
Fragments arranged by size:
1943 1773 716 209 181 155 23
BssSI C'ACGA_G
Cuts at: 3271 3629 3271
Size: 358 4642
BstAPI GCAn_nnn'nTGC
Cuts at: 2377 2377
Size: 5000
BstBI TT'CG_AA
Cuts at: 110 3361 110
Size: 3251 1749
BstEII G'GTnAC_C
Cuts at: 334 334
Size: 5000
BstYI r'GATC_y
Cuts at: 1 980 1541 1614 1806 2025 2492 2515 2850
Size: 979 561 73 192 219 467 23 335
Cuts at: 2850 3096 4108 4194 4206 4552 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 979 561 467 449 346 335 246
219 192 86 73 23 12
Bsu36I CC'TnA_GG
Cuts at: 287 308 1886 287
Size: 21 1578 3401
Fragments arranged by size:
3401 1578 21
BtgI C'CryG_G
Cuts at: 125 139 125
Size: 14 4986
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2950 3131 3294 2950
Size: 181 163 4656
Fragments arranged by size:
4656 181 163
BtsI GCAGTG_nn'
Cuts at: 2243 2243
Size: 5000
DraI TTT'AAA
Cuts at: 1999 1999
Size: 5000
DraIII CAC_nnn'GTG
Cuts at: 480 480
Size: 5000
DrdI GACnn_nn'nnGTC
Cuts at: 2706 3564 2706
Size: 858 4142
EaeI y'GGCC_r
Cuts at: 2585 2759 3150 3177 4522 2585
Size: 174 391 27 1345 3063
Fragments arranged by size:
3063 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2585 2585
Size: 5000
EarI CTCTTCn'nnn_
Cuts at: 251 692 1253 1687 3023 3233 251
Size: 441 561 434 1336 210 2018
Fragments arranged by size:
2018 1336 561 441 434 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3208 3518 3664 4810 3208
Size: 310 146 1146 3398
Fragments arranged by size:
3398 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2012 2012
Size: 5000
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 713 1025 1031 1687 2825 3257 4004 4272 713
Size: 312 6 656 1138 432 747 268 1441
Fragments arranged by size:
1441 1138 747 656 432 312 268 6
EcoO109I rG'GnC_Cy
Cuts at: 379 2139 379
Size: 1760 3240
EcoRI G'AATT_C
Cuts at: 2004 2004
Size: 5000
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 721 936 1759 3019 721
Size: 215 823 1260 2702
Fragments arranged by size:
2702 1260 823 215
FspI TGC'GCA
Cuts at: 502 2781 502
Size: 2279 2721
HaeII r_GCGC'y
Cuts at: 175 242 250 271 2434 2682 3704 4683 175
Size: 67 8 21 2163 248 1022 979 492
Fragments arranged by size:
2163 1022 979 492 248 67 21 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 442 474 514 546 1132 1164 1231 1263 1923
Size: 32 40 32 586 32 67 32 660
Cuts at: 1923 1955 2842 2872 2874 2904 442
Size: 32 887 30 2 30 2538
Fragments arranged by size:
2538 887 660 586 67 40 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1533 2039 1533
Size: 506 4494
KasI G'GCGC_C
Cuts at: 2430 2678 2430
Size: 248 4752
KpnI G_GTAC'C
Cuts at: 510 2020 510
Size: 1510 3490
MluI A'CGCG_T
Cuts at: 4433 4433
Size: 5000
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3671 3855 3671
Size: 184 4816
MscI TGG'CCA
Cuts at: 2761 2761
Size: 5000
MslI CAynn'nnrTG
Cuts at: 2989 3116 2989
Size: 127 4873
MspA1I CmG'CkG
Cuts at: 949 2111 2425 2785 3798 4043 949
Size: 1162 314 360 1013 245 1906
Fragments arranged by size:
1906 1162 1013 360 314 245
NaeI GCC'GGC
Cuts at: 356 3181 356
Size: 2825 2175
NarI GG'CG_CC
Cuts at: 2431 2679 2431
Size: 248 4752
NcoI C'CATG_G
Cuts at: 125 125
Size: 5000
NgoMIV G'CCGG_C
Cuts at: 354 3179 354
Size: 2825 2175
NsiI A_TGCA'T
Cuts at: 1466 1466
Size: 5000
NspI r_CATG'y
Cuts at: 1564 2053 2553 3460 1564
Size: 489 500 907 3104
Fragments arranged by size:
3104 907 500 489
PasI CC'CwG_GG
Cuts at: 345 345
Size: 5000
PciI A'CATG_T
Cuts at: 3456 3456
Size: 5000
PflMI CCAn_nnn'nTGG
Cuts at: 345 786 345
Size: 441 4559
PmeI GTTT'AAAC
Cuts at: 1999 1999
Size: 5000
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4139 4171 4139
Size: 32 4968
PspOMI G'GGCC_C
Cuts at: 379 379
Size: 5000
PstI C_TGCA'G
Cuts at: 1254 1806 2047 2732 1254
Size: 552 241 685 3522
Fragments arranged by size:
3522 685 552 241
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5000
PvuII CAG'CTG
Cuts at: 949 2425 2785 949
Size: 1476 360 3164
Fragments arranged by size:
3164 1476 360
RsrII CG'GwC_CG
Cuts at: 3195 3195
Size: 5000
SacI G_AGCT'C
Cuts at: 2014 2014
Size: 5000
SalI G'TCGA_C
Cuts at: 2037 2037
Size: 5000
SapI GCTCTTCn'nnn_
Cuts at: 1687 3023 3233 1687
Size: 1336 210 3454
Fragments arranged by size:
3454 1336 210
SbfI CC_TGCA'GG
Cuts at: 2047 2047
Size: 5000
ScaI AGT'ACT
Cuts at: 1672 4517 1672
Size: 2845 2155
SexAI A'CCwGG_T
Cuts at: 476 476
Size: 5000
SfcI C'TryA_G
Cuts at: 33 863 1250 1499 1802 2043 2728 3721 3912
Size: 830 387 249 303 241 685 993 191
Cuts at: 3912 33
Size: 1121
Fragments arranged by size:
1121 993 830 685 387 303 249 241
191
SfoI GGC'GCC
Cuts at: 2432 2680 2432
Size: 248 4752
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5000
SmaI CCC'GGG
Cuts at: 244 326 2022 244
Size: 82 1696 3222
Fragments arranged by size:
3222 1696 82
SmlI C'TyrA_G
Cuts at: 770 841 1275 2155 3070 3562 3824 770
Size: 71 434 880 915 492 262 1946
Fragments arranged by size:
1946 915 880 492 434 262 71
SnaBI TAC'GTA
Cuts at: 1992 1992
Size: 5000
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5000
SphI G_CATG'C
Cuts at: 2053 2553 2053
Size: 500 4500
SrfI GCCC'GGGC
Cuts at: 244 244
Size: 5000
StyI C'CwwG_G
Cuts at: 125 2134 2488 125
Size: 2009 354 2637
Fragments arranged by size:
2637 2009 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2583 2716 3175 3384 2583
Size: 133 459 209 4199
Fragments arranged by size:
4199 459 209 133
TatI w'GTAC_w
Cuts at: 823 1670 4515 823
Size: 847 2845 1308
Fragments arranged by size:
2845 1308 847
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1438 1470 4517 4549 1438
Size: 32 3047 32 1889
Fragments arranged by size:
3047 1889 32 32
Tth111I GACn'n_nGTC
Cuts at: 2797 2797
Size: 5000
XbaI T'CTAG_A
Cuts at: 2031 2031
Size: 5000
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1818 2495 1818
Size: 677 4323
XmaI C'CCGG_G
Cuts at: 242 324 2020 242
Size: 82 1696 3222
Fragments arranged by size:
3222 1696 82
XmnI GAAnn'nnTTC
Cuts at: 1196 1739 1196
Size: 543 4457
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SrfI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BcgI BmgBI BmtI BpmI BsiWI BsrGI BssHII
BstXI BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SfiI SgrAI SspI StuI SwaI XhoI ZraI