Restriction map for
pF1KSDA1277
(Circular) (Six-base) MAPSORT of: pF1KSDA1277.seq Check: 9341 from: 1 to: 6164
pF1KSDA1277 6164 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AatII G_ACGT'C
Cuts at: 2884 2884
Size: 6164
AccI GT'mk_AC
Cuts at: 2546 3202 2546
Size: 656 5508
Acc65I G'GTAC_C
Cuts at: 1769 3180 1769
Size: 1411 4753
AclI AA'CG_TT
Cuts at: 940 6004 940
Size: 5064 1100
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 975 1774 1921 1969 2130 2132 2692 2846
Size: 350 799 147 48 161 2 560 154
Cuts at: 2846 3133 3989 4421 5168 5436 625
Size: 287 856 432 747 268 1353
Fragments arranged by size:
1353 856 799 747 560 432 350 287
268 161 154 147 48 2
AflIII A'CryG_T
Cuts at: 872 3516 4620 5597 872
Size: 2644 1104 977 1439
Fragments arranged by size:
2644 1439 1104 977
AgeI A'CCGG_T
Cuts at: 4552 4552
Size: 6164
AhdI GACnn_n'nnGTC
Cuts at: 2355 2355
Size: 6164
AleI CACnn'nnGTG
Cuts at: 3095 3095
Size: 6164
AlwNI CAG_nnn'CTG
Cuts at: 1731 2299 3469 5036 1731
Size: 568 1170 1567 2859
Fragments arranged by size:
2859 1567 1170 568
ApaI G_GGCC'C
Cuts at: 2774 2774
Size: 6164
ApaLI G'TGCA_C
Cuts at: 4934 4934
Size: 6164
ApoI r'AATT_y
Cuts at: 1323 1404 3136 3168 5517 5773 1323
Size: 81 1732 32 2349 256 1714
Fragments arranged by size:
2349 1732 1714 256 81 32
AseI AT'TA_AT
Cuts at: 20 20
Size: 6164
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6164
AvaI C'yCGr_G
Cuts at: 622 1784 2338 3184 622
Size: 1162 554 846 3602
Fragments arranged by size:
3602 1162 846 554
BamHI G'GATC_C
Cuts at: 531 657 3189 531
Size: 126 2532 3506
Fragments arranged by size:
3506 2532 126
BanI G'GyrC_C
Cuts at: 300 1761 1769 3180 3594 3842 3877 300
Size: 1461 8 1411 414 248 35 2587
Fragments arranged by size:
2587 1461 1411 414 248 35 8
BanII G_rGCy'C
Cuts at: 806 886 2774 3057 3178 4208 806
Size: 80 1888 283 121 1030 2762
Fragments arranged by size:
2762 1888 1030 283 121 80
BbeI G_GCGC'C
Cuts at: 1765 3598 3846 1765
Size: 1833 248 4083
Fragments arranged by size:
4083 1833 248
BbsI GAAGACnn'nnnn_
Cuts at: 1086 1145 1244 2082 2961 1086
Size: 59 99 838 879 4289
Fragments arranged by size:
4289 879 838 99 59
BbvCI CC'TCA_GC
Cuts at: 1937 2374 1937
Size: 437 5727
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1747 1781 2043 2077 1747
Size: 34 262 34 5834
Fragments arranged by size:
5834 262 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4062 4823 6164 4062
Size: 761 1341 4062
Fragments arranged by size:
4062 1341 761
BclI T'GATC_A
Cuts at: 149 3684 149
Size: 3535 2629
BglI GCCn_nnn'nGGC
Cuts at: 181 181
Size: 6164
BglII A'GATC_T
Cuts at: 3679 5716 3679
Size: 2037 4127
BlpI GC'TnA_GC
Cuts at: 2449 3276 2449
Size: 827 5337
Bme1580I G_kGCm'C
Cuts at: 305 2774 3789 3882 4938 305
Size: 2469 1015 93 1056 1531
Fragments arranged by size:
2469 1531 1056 1015 93
BmrI ACTGGGnnnn_n'
Cuts at: 657 1472 1632 1956 3074 3376 3457 3792 5936
Size: 815 160 324 1118 302 81 335 2144
Cuts at: 5936 657
Size: 885
Fragments arranged by size:
2144 1118 885 815 335 324 302 160
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1671 4531 1671
Size: 2860 3304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2060 2108 2437 2574 2983 2060
Size: 48 329 137 409 5241
Fragments arranged by size:
5241 409 329 137 48
Bpu10I CC'TnA_GC
Cuts at: 508 812 1937 2374 2608 508
Size: 304 1125 437 234 4064
Fragments arranged by size:
4064 1125 437 304 234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 886 3340 4219 4711 5009 886
Size: 2454 879 492 298 2041
Fragments arranged by size:
2454 2041 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1526 5868 1526
Size: 4342 1822
BsaAI yAC'GTr
Cuts at: 1872 2346 3156 3517 1872
Size: 474 810 361 4519
Fragments arranged by size:
4519 810 474 361
BsaBI GATnn'nnATC
Cuts at: 6 3678 5715 6
Size: 3672 2037 455
Fragments arranged by size:
3672 2037 455
BsaHI Gr'CG_yC
Cuts at: 1762 2566 2881 3595 3843 4545 6041 1762
Size: 804 315 714 248 702 1496 1885
Fragments arranged by size:
1885 1496 804 714 702 315 248
BsaWI w'CCGG_w
Cuts at: 857 1604 3353 3874 4552 4826 4973 5384 857
Size: 747 1749 521 678 274 147 411 1637
Fragments arranged by size:
1749 1637 747 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 363 393 363
Size: 30 6134
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 384 387 1007 1041 1237 1428 1717 2358 2436
Size: 3 620 34 196 191 289 641 78
Cuts at: 2436 2911 2928 384
Size: 475 17 3620
Fragments arranged by size:
3620 641 620 475 289 196 191 78
34 17 3
BseYI C'CCAG_C
Cuts at: 162 184 316 539 1197 1498 2247 3589 4347
Size: 22 132 223 658 301 749 1342 758
Cuts at: 4347 4924 5420 162
Size: 577 496 906
Fragments arranged by size:
1342 906 758 749 658 577 496 301
223 132 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 296 1130 296
Size: 834 5330
BsiEI CG_ry'CG
Cuts at: 104 3706 3752 4960 104
Size: 3602 46 1208 1308
Fragments arranged by size:
3602 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 442 2345 2560 3092 3178 3956 4146 4938 442
Size: 1903 215 532 86 778 190 792 1668
Fragments arranged by size:
1903 1668 792 778 532 215 190 86
Bsp1286I G_dGCh'C
Cuts at: 305 442 806 886 2345 2560 2774 3057 3092
Size: 137 364 80 1459 215 214 283 35
Cuts at: 3092 3178 3789 3882 3956 4146 4208 4938 305
Size: 86 611 93 74 190 62 730 1531
Fragments arranged by size:
1531 1459 730 611 364 283 215 214
190 137 93 86 80 74 62 35
BspEI T'CCGG_A
Cuts at: 1604 5384 1604
Size: 3780 2384
BspHI T'CATG_A
Cuts at: 1925 2219 5340 1925
Size: 294 3121 2749
Fragments arranged by size:
3121 2749 294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 600 1395 3214 3428 3730 4111 600
Size: 795 1819 214 302 381 2653
Fragments arranged by size:
2653 1819 795 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3009 4456 4510 5994 6159 50
Size: 2959 1447 54 1484 165 55
Fragments arranged by size:
2959 1484 1447 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2435 4076 2435
Size: 1641 4523
BsrFI r'CCGG_y
Cuts at: 2657 3020 4162 4343 4552 2657
Size: 363 1142 181 209 4269
Fragments arranged by size:
4269 1142 363 209 181
BssSI C'ACGA_G
Cuts at: 4435 4793 4435
Size: 358 5806
BstAPI GCAn_nnn'nTGC
Cuts at: 3541 3541
Size: 6164
BstBI TT'CG_AA
Cuts at: 110 4525 110
Size: 4415 1749
BstYI r'GATC_y
Cuts at: 1 531 657 1248 2162 3189 3656 3679 4014
Size: 530 126 591 914 1027 467 23 335
Cuts at: 4014 4260 5272 5358 5370 5716 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1027 1012 914 591 530 467 449 346
335 246 126 86 23 12
BtgI C'CryG_G
Cuts at: 125 2540 125
Size: 2415 3749
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2491 4114 4295 4458 2491
Size: 1623 181 163 4197
Fragments arranged by size:
4197 1623 181 163
BtsI GCAGTG_nn'
Cuts at: 473 690 2602 3407 473
Size: 217 1912 805 3230
Fragments arranged by size:
3230 1912 805 217
DraI TTT'AAA
Cuts at: 3163 3163
Size: 6164
DrdI GACnn_nn'nnGTC
Cuts at: 3870 4728 3870
Size: 858 5306
EaeI y'GGCC_r
Cuts at: 3749 3923 4314 4341 5686 3749
Size: 174 391 27 1345 4227
Fragments arranged by size:
4227 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3749 3749
Size: 6164
EarI CTCTTCn'nnn_
Cuts at: 1350 1549 1621 1822 2368 2621 4187 4397 1350
Size: 199 72 201 546 253 1566 210 3117
Fragments arranged by size:
3117 1566 546 253 210 201 199 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1241 2371 2941 4372 4682 4828 5974 1241
Size: 1130 570 1431 310 146 1146 1431
Fragments arranged by size:
1431 1431 1146 1130 570 310 146
EcoICRI GAG'CTC
Cuts at: 3176 3176
Size: 6164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 975 1774 1921 1969 2060 2108 2130 2132
Size: 350 799 147 48 91 48 22 2
Cuts at: 2132 2437 2574 2692 2846 2983 3133 3989 4421
Size: 305 137 118 154 137 150 856 432
Cuts at: 4421 5168 5436 625
Size: 747 268 1353
Fragments arranged by size:
1353 856 799 747 432 350 305 268
154 150 147 137 137 118 91 48
48 22 2
EcoO109I rG'GnC_Cy
Cuts at: 486 848 1466 2631 2770 2771 3303 486
Size: 362 618 1165 139 1 532 3347
Fragments arranged by size:
3347 1165 618 532 362 139 1
EcoRI G'AATT_C
Cuts at: 3168 3168
Size: 6164
EcoRV GAT'ATC
Cuts at: 275 275
Size: 6164
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 594 1335 2650 4183 594
Size: 741 1315 1533 2575
Fragments arranged by size:
2575 1533 1315 741
FspI TGC'GCA
Cuts at: 3945 3945
Size: 6164
HaeII r_GCGC'y
Cuts at: 1731 1765 2106 2488 3598 3846 4868 5847 1731
Size: 34 341 382 1110 248 1022 979 2048
Fragments arranged by size:
2048 1110 1022 979 382 341 248 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 259 291 580 612 703 735 2177 2209 2341
Size: 32 289 32 91 32 1442 32 132
Cuts at: 2341 2373 2671 2703 2806 2838 4006 4036 4038
Size: 32 298 32 103 32 1168 30 2
Cuts at: 4038 4068 259
Size: 30 2355
Fragments arranged by size:
2355 1442 1168 298 289 132 103 91
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1737 2547 2643 2652 3203 1737
Size: 810 96 9 551 4698
Fragments arranged by size:
4698 810 551 96 9
HindIII A'AGCT_T
Cuts at: 2704 2704
Size: 6164
KasI G'GCGC_C
Cuts at: 1761 3594 3842 1761
Size: 1833 248 4083
Fragments arranged by size:
4083 1833 248
KpnI G_GTAC'C
Cuts at: 1773 3184 1773
Size: 1411 4753
MluI A'CGCG_T
Cuts at: 5597 5597
Size: 6164
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1422 1886 4835 5019 1422
Size: 464 2949 184 2567
Fragments arranged by size:
2949 2567 464 184
MscI TGG'CCA
Cuts at: 3925 3925
Size: 6164
MslI CAynn'nnrTG
Cuts at: 130 2524 3095 4153 4280 130
Size: 2394 571 1058 127 2014
Fragments arranged by size:
2394 2014 1058 571 127
MspA1I CmG'CkG
Cuts at: 166 2325 2622 2913 3095 3275 3589 3949 4962
Size: 2159 297 291 182 180 314 360 1013
Cuts at: 4962 5207 166
Size: 245 1123
Fragments arranged by size:
2159 1123 1013 360 314 297 291 245
182 180
NaeI GCC'GGC
Cuts at: 3022 4345 3022
Size: 1323 4841
NarI GG'CG_CC
Cuts at: 1762 3595 3843 1762
Size: 1833 248 4083
Fragments arranged by size:
4083 1833 248
NcoI C'CATG_G
Cuts at: 125 2540 125
Size: 2415 3749
NgoMIV G'CCGG_C
Cuts at: 3020 4343 3020
Size: 1323 4841
NspI r_CATG'y
Cuts at: 952 3217 3717 4624 952
Size: 2265 500 907 2492
Fragments arranged by size:
2492 2265 907 500
PasI CC'CwG_GG
Cuts at: 851 2253 851
Size: 1402 4762
PciI A'CATG_T
Cuts at: 4620 4620
Size: 6164
PflMI CCAn_nnn'nTGG
Cuts at: 565 1644 1731 1988 2478 2961 565
Size: 1079 87 257 490 483 3768
Fragments arranged by size:
3768 1079 490 483 257 87
PfoI T'CCnGG_A
Cuts at: 2717 2960 3002 2717
Size: 243 42 5879
Fragments arranged by size:
5879 243 42
PmeI GTTT'AAAC
Cuts at: 3163 3163
Size: 6164
PmlI CAC'GTG
Cuts at: 2346 2346
Size: 6164
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5303 5335 5303
Size: 32 6132
PpuMI rG'GwC_Cy
Cuts at: 486 1466 2631 486
Size: 980 1165 4019
Fragments arranged by size:
4019 1165 980
PspOMI G'GGCC_C
Cuts at: 2770 2770
Size: 6164
PspXI vC'TCGA_Gb
Cuts at: 622 2338 622
Size: 1716 4448
PstI C_TGCA'G
Cuts at: 171 1151 2912 3211 3896 171
Size: 980 1761 299 685 2439
Fragments arranged by size:
2439 1761 980 685 299
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6164
PvuII CAG'CTG
Cuts at: 166 3589 3949 166
Size: 3423 360 2381
Fragments arranged by size:
3423 2381 360
RsrII CG'GwC_CG
Cuts at: 4359 4359
Size: 6164
SacI G_AGCT'C
Cuts at: 3178 3178
Size: 6164
SalI G'TCGA_C
Cuts at: 2545 3201 2545
Size: 656 5508
SanDI GG'GwC_CC
Cuts at: 1466 1466
Size: 6164
SapI GCTCTTCn'nnn_
Cuts at: 1822 4187 4397 1822
Size: 2365 210 3589
Fragments arranged by size:
3589 2365 210
SbfI CC_TGCA'GG
Cuts at: 3211 3211
Size: 6164
ScaI AGT'ACT
Cuts at: 1825 5681 1825
Size: 3856 2308
SexAI A'CCwGG_T
Cuts at: 2387 2387
Size: 6164
SfcI C'TryA_G
Cuts at: 33 167 1147 2908 3207 3892 4885 5076 33
Size: 134 980 1761 299 685 993 191 1121
Fragments arranged by size:
1761 1121 993 980 685 299 191 134
SfoI GGC'GCC
Cuts at: 1763 3596 3844 1763
Size: 1833 248 4083
Fragments arranged by size:
4083 1833 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6164
SmaI CCC'GGG
Cuts at: 1786 3186 1786
Size: 1400 4764
SmlI C'TyrA_G
Cuts at: 622 865 2338 3319 4234 4726 4988 622
Size: 243 1473 981 915 492 262 1798
Fragments arranged by size:
1798 1473 981 915 492 262 243
SnaBI TAC'GTA
Cuts at: 3156 3156
Size: 6164
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6164
SphI G_CATG'C
Cuts at: 3217 3717 3217
Size: 500 5664
StuI AGG'CCT
Cuts at: 2920 2920
Size: 6164
StyI C'CwwG_G
Cuts at: 125 1527 1982 2540 3298 3652 125
Size: 1402 455 558 758 354 2637
Fragments arranged by size:
2637 1402 758 558 455 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 330 1900 3747 3880 4339 4548 330
Size: 1570 1847 133 459 209 1946
Fragments arranged by size:
1946 1847 1570 459 209 133
TatI w'GTAC_w
Cuts at: 1823 5679 1823
Size: 3856 2308
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 363 395 1108 1140 2288 2320 5681 5713 363
Size: 32 713 32 1148 32 3361 32 814
Fragments arranged by size:
3361 1148 814 713 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2647 3961 2647
Size: 1314 4850
XbaI T'CTAG_A
Cuts at: 1245 3195 1245
Size: 1950 4214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3659 3659
Size: 6164
XhoI C'TCGA_G
Cuts at: 622 2338 622
Size: 1716 4448
XmaI C'CCGG_G
Cuts at: 1784 3184 1784
Size: 1400 4764
XmnI GAAnn'nnTTC
Cuts at: 1241 2726 1241
Size: 1485 4679
ZraI GAC'GTC
Cuts at: 2882 2882
Size: 6164
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AlfI AloI AscI AvrII BaeI
BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PshAI PsiI PsrI SacII SfiI SgrAI
SrfI SspI SwaI