Restriction map for
pF1KSDA1276
(Circular) (Six-base) MAPSORT of: pF1KSDA1276.seq Check: 5000 from: 1 to: 6317
pF1KSDA1276 6317 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 3190 3355 3190
Size: 165 6152
Acc65I G'GTAC_C
Cuts at: 3333 3333
Size: 6317
AclI AA'CG_TT
Cuts at: 6157 6157
Size: 6317
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1003 1024 1808 2830 2896 4142 4574 5321 5589
Size: 21 784 1022 66 1246 432 747 268
Cuts at: 5589 1003
Size: 1731
Fragments arranged by size:
1731 1246 1022 784 747 432 268 66
21
AflII C'TTAA_G
Cuts at: 1036 1036
Size: 6317
AflIII A'CryG_T
Cuts at: 3669 4773 5750 3669
Size: 1104 977 4236
Fragments arranged by size:
4236 1104 977
AgeI A'CCGG_T
Cuts at: 4705 4705
Size: 6317
AleI CACnn'nnGTG
Cuts at: 265 265
Size: 6317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1172 1306 2366 1172
Size: 134 1060 5123
Fragments arranged by size:
5123 1060 134
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3052 3084 3052
Size: 32 6285
AlwNI CAG_nnn'CTG
Cuts at: 435 618 732 753 855 1002 1023 1666 1971
Size: 183 114 21 102 147 21 643 305
Cuts at: 1971 2307 2334 3622 5189 435
Size: 336 27 1288 1567 1563
Fragments arranged by size:
1567 1563 1288 643 336 305 183 147
114 102 27 21 21
ApaI G_GGCC'C
Cuts at: 697 697
Size: 6317
ApaLI G'TGCA_C
Cuts at: 1980 5087 1980
Size: 3107 3210
ApoI r'AATT_y
Cuts at: 1056 1599 1630 3321 5670 5926 1056
Size: 543 31 1691 2349 256 1447
Fragments arranged by size:
2349 1691 1447 543 256 31
AseI AT'TA_AT
Cuts at: 20 3113 20
Size: 3093 3224
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6317
AvaI C'yCGr_G
Cuts at: 2101 2484 2836 2988 3337 2101
Size: 383 352 152 349 5081
Fragments arranged by size:
5081 383 352 349 152
BamHI G'GATC_C
Cuts at: 1722 3342 1722
Size: 1620 4697
BanI G'GyrC_C
Cuts at: 193 2363 3333 3747 3995 4030 193
Size: 2170 970 414 248 35 2480
Fragments arranged by size:
2480 2170 970 414 248 35
BanII G_rGCy'C
Cuts at: 178 347 587 667 697 1870 2060 2124 2237
Size: 169 240 80 30 1173 190 64 113
Cuts at: 2237 2406 2546 3268 3331 4361 178
Size: 169 140 722 63 1030 2134
Fragments arranged by size:
2134 1173 1030 722 240 190 169 169
140 113 80 64 63 30
BbeI G_GCGC'C
Cuts at: 197 3751 3999 197
Size: 3554 248 2515
Fragments arranged by size:
3554 2515 248
BbsI GAAGACnn'nnnn_
Cuts at: 1171 1355 1430 1171
Size: 184 75 6058
Fragments arranged by size:
6058 184 75
BbvCI CC'TCA_GC
Cuts at: 302 526 1235 1667 2398 302
Size: 224 709 432 731 4221
Fragments arranged by size:
4221 731 709 432 224
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 231 265 1117 1151 231
Size: 34 852 34 5397
Fragments arranged by size:
5397 852 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4215 4976 6317 4215
Size: 761 1341 4215
Fragments arranged by size:
4215 1341 761
BclI T'GATC_A
Cuts at: 3837 3837
Size: 6317
BglI GCCn_nnn'nGGC
Cuts at: 775 922 2495 2674 2731 2773 775
Size: 147 1573 179 57 42 4319
Fragments arranged by size:
4319 1573 179 147 57 42
BglII A'GATC_T
Cuts at: 3832 5869 3832
Size: 2037 4280
BlpI GC'TnA_GC
Cuts at: 754 3429 754
Size: 2675 3642
Bme1580I G_kGCm'C
Cuts at: 697 1267 1984 2366 2751 3038 3942 4035 5091
Size: 570 717 382 385 287 904 93 1056
Cuts at: 5091 697
Size: 1923
Fragments arranged by size:
1923 1056 904 717 570 385 382 287
93
BmgBI CAC'GTC
Cuts at: 3033 3033
Size: 6317
BmrI ACTGGGnnnn_n'
Cuts at: 1873 2936 3529 3610 3945 6089 1873
Size: 1063 593 81 335 2144 2101
Fragments arranged by size:
2144 2101 1063 593 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 510 4684 510
Size: 4174 2143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 331 457 469 526 552 595 601 973
Size: 109 126 12 57 26 43 6 372
Cuts at: 973 1579 1732 1993 2044 2053 2155 2188 2221
Size: 606 153 261 51 9 102 33 33
Cuts at: 2221 2251 2551 2671 2746 222
Size: 30 300 120 75 3793
Fragments arranged by size:
3793 606 372 300 261 153 126 120
109 102 75 57 51 43 33 33
30 26 12 9 6
Bpu10I CC'TnA_GC
Cuts at: 302 526 926 1235 1667 2398 302
Size: 224 400 309 432 731 4221
Fragments arranged by size:
4221 731 432 400 309 224
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 1240 1630 1819 1999 2143 3493 4372 4864
Size: 669 390 189 180 144 1350 879 492
Cuts at: 4864 5162 571
Size: 298 1726
Fragments arranged by size:
1726 1350 879 669 492 390 298 189
180 144
BsaI GGTCTCn'nnnn_
Cuts at: 358 941 1708 1956 2059 2627 6021 358
Size: 583 767 248 103 568 3394 654
Fragments arranged by size:
3394 767 654 583 568 248 103
BsaAI yAC'GTr
Cuts at: 231 3309 3670 231
Size: 3078 361 2878
Fragments arranged by size:
3078 2878 361
BsaBI GATnn'nnATC
Cuts at: 6 3831 5868 6
Size: 3825 2037 455
Fragments arranged by size:
3825 2037 455
BsaHI Gr'CG_yC
Cuts at: 194 3748 3996 4698 6194 194
Size: 3554 248 702 1496 317
Fragments arranged by size:
3554 1496 702 317 248
BsaWI w'CCGG_w
Cuts at: 932 2708 2803 2957 3506 4027 4705 4979 5126
Size: 1776 95 154 549 521 678 274 147
Cuts at: 5126 5537 932
Size: 411 1712
Fragments arranged by size:
1776 1712 678 549 521 411 274 154
147 95
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 862 892 2219 2249 3052 3082 862
Size: 30 1327 30 803 30 4097
Fragments arranged by size:
4097 1327 803 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 355 418 487 618 796 1396 1772 1777 1924
Size: 63 69 131 178 600 376 5 147
Cuts at: 1924 2290 2485 2566 2668 355
Size: 366 195 81 102 4004
Fragments arranged by size:
4004 600 376 366 195 178 147 131
102 81 69 63 5
BseYI C'CCAG_C
Cuts at: 254 2096 2515 2669 2756 3037 3139 3157 3251
Size: 1842 419 154 87 281 102 18 94
Cuts at: 3251 3742 4500 5077 5573 254
Size: 491 758 577 496 998
Fragments arranged by size:
1842 998 758 577 496 491 419 281
154 102 94 87 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 519 745 1051 519
Size: 226 306 5785
Fragments arranged by size:
5785 306 226
BsiEI CG_ry'CG
Cuts at: 104 3859 3905 5113 104
Size: 3755 46 1208 1308
Fragments arranged by size:
3755 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 347 545 587 959 1984 2060 2237 3331 4109
Size: 198 42 372 1025 76 177 1094 778
Cuts at: 4109 4299 5091 347
Size: 190 792 1573
Fragments arranged by size:
1573 1094 1025 792 778 372 198 190
177 76 42
BsmI GAATG_Cn'
Cuts at: 357 1706 2935 357
Size: 1349 1229 3739
Fragments arranged by size:
3739 1349 1229
BsmBI CGTCTCn'nnnn_
Cuts at: 502 502
Size: 6317
Bsp1286I G_dGCh'C
Cuts at: 178 347 545 587 667 697 959 1267 1870
Size: 169 198 42 80 30 262 308 603
Cuts at: 1870 1984 2060 2124 2237 2366 2406 2546 2751
Size: 114 76 64 113 129 40 140 205
Cuts at: 2751 3038 3268 3331 3942 4035 4109 4299 4361
Size: 287 230 63 611 93 74 190 62
Cuts at: 4361 5091 178
Size: 730 1404
Fragments arranged by size:
1404 730 611 603 308 287 262 230
205 198 190 169 140 129 114 113
93 80 76 74 64 63 62 42
40 30
BspEI T'CCGG_A
Cuts at: 932 2803 5537 932
Size: 1871 2734 1712
Fragments arranged by size:
2734 1871 1712
BspHI T'CATG_A
Cuts at: 5493 5493
Size: 6317
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2012 2633 3367 3581 3883 4264 2012
Size: 621 734 214 302 381 4065
Fragments arranged by size:
4065 734 621 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4609 4663 6147 6312 50
Size: 4559 54 1484 165 55
Fragments arranged by size:
4559 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4229 4229
Size: 6317
BsrFI r'CCGG_y
Cuts at: 196 647 1219 2494 4315 4496 4705 196
Size: 451 572 1275 1821 181 209 1808
Fragments arranged by size:
1821 1808 1275 572 451 209 181
BssHII G'CGCG_C
Cuts at: 2589 2589
Size: 6317
BssSI C'ACGA_G
Cuts at: 495 628 4588 4946 495
Size: 133 3960 358 1866
Fragments arranged by size:
3960 1866 358 133
BstAPI GCAn_nnn'nTGC
Cuts at: 732 831 2268 2307 3205 3694 732
Size: 99 1437 39 898 489 3355
Fragments arranged by size:
3355 1437 898 489 99 39
BstBI TT'CG_AA
Cuts at: 110 4678 110
Size: 4568 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 2685 3045 2685
Size: 360 5957
BstYI r'GATC_y
Cuts at: 1 1722 2207 3342 3809 3832 4167 4413 5425
Size: 1721 485 1135 467 23 335 246 1012
Cuts at: 5425 5511 5523 5869 1
Size: 86 12 346 449
Fragments arranged by size:
1721 1135 1012 485 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 125 1445 1472 2726 125
Size: 1320 27 1254 3716
Fragments arranged by size:
3716 1320 1254 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4267 4448 4611 4267
Size: 181 163 5973
Fragments arranged by size:
5973 181 163
BtsI GCAGTG_nn'
Cuts at: 868 1073 1698 1809 1984 2383 2692 3560 868
Size: 205 625 111 175 399 309 868 3625
Fragments arranged by size:
3625 868 625 399 309 205 175 111
DraI TTT'AAA
Cuts at: 3316 3316
Size: 6317
DraIII CAC_nnn'GTG
Cuts at: 1446 1446
Size: 6317
DrdI GACnn_nn'nnGTC
Cuts at: 4023 4881 4023
Size: 858 5459
EaeI y'GGCC_r
Cuts at: 2856 3165 3902 4076 4467 4494 5839 2856
Size: 309 737 174 391 27 1345 3334
Fragments arranged by size:
3334 1345 737 391 309 174 27
EagI C'GGCC_G
Cuts at: 3902 3902
Size: 6317
EarI CTCTTCn'nnn_
Cuts at: 454 1196 1373 1562 2906 4340 4550 454
Size: 742 177 189 1344 1434 210 2221
Fragments arranged by size:
2221 1434 1344 742 210 189 177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4525 4835 4981 6127 4525
Size: 310 146 1146 4715
Fragments arranged by size:
4715 1146 310 146
EcoICRI GAG'CTC
Cuts at: 345 585 2058 2235 3329 345
Size: 240 1473 177 1094 3333
Fragments arranged by size:
3333 1473 1094 240 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 331 457 469 526 552 595 601 973
Size: 109 126 12 57 26 43 6 372
Cuts at: 973 1003 1024 1579 1732 1808 1993 2044 2053
Size: 30 21 555 153 76 185 51 9
Cuts at: 2053 2155 2188 2221 2251 2551 2671 2746 2830
Size: 102 33 33 30 300 120 75 84
Cuts at: 2830 2896 4142 4574 5321 5589 222
Size: 66 1246 432 747 268 950
Fragments arranged by size:
1246 950 747 555 432 372 300 268
185 153 126 120 109 102 84 76
75 66 57 51 43 33 33 30
30 26 21 12 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 498 718 801 498
Size: 220 83 6014
Fragments arranged by size:
6014 220 83
EcoO109I rG'GnC_Cy
Cuts at: 432 693 711 852 2001 2304 2841 3215 3456
Size: 261 18 141 1149 303 537 374 241
Cuts at: 3456 432
Size: 3293
Fragments arranged by size:
3293 1149 537 374 303 261 241 141
18
EcoRI G'AATT_C
Cuts at: 1056 1599 3321 1056
Size: 543 1722 4052
Fragments arranged by size:
4052 1722 543
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1369 4336 1369
Size: 2967 3350
FspI TGC'GCA
Cuts at: 399 2128 4098 399
Size: 1729 1970 2618
Fragments arranged by size:
2618 1970 1729
HaeII r_GCGC'y
Cuts at: 197 322 446 481 2513 3751 3999 5021 6000
Size: 125 124 35 2032 1238 248 1022 979
Cuts at: 6000 197
Size: 514
Fragments arranged by size:
2032 1238 1022 979 514 248 125 124
35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 862 894 1707 1739 1873 1905 2219 2251 2528
Size: 32 813 32 134 32 314 32 277
Cuts at: 2528 2560 2876 2908 3081 3113 4159 4189 4191
Size: 32 316 32 173 32 1046 30 2
Cuts at: 4191 4221 862
Size: 30 2958
Fragments arranged by size:
2958 1046 813 316 314 277 173 134
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3356 3356
Size: 6317
KasI G'GCGC_C
Cuts at: 193 3747 3995 193
Size: 3554 248 2515
Fragments arranged by size:
3554 2515 248
KpnI G_GTAC'C
Cuts at: 3337 3337
Size: 6317
MluI A'CGCG_T
Cuts at: 5750 5750
Size: 6317
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 659 860 1499 1662 2235 2318 4988 5172 659
Size: 201 639 163 573 83 2670 184 1804
Fragments arranged by size:
2670 1804 639 573 201 184 163 83
MscI TGG'CCA
Cuts at: 4078 4078
Size: 6317
MslI CAynn'nnrTG
Cuts at: 265 1025 1288 4306 4433 265
Size: 760 263 3018 127 2149
Fragments arranged by size:
3018 2149 760 263 127
MspA1I CmG'CkG
Cuts at: 729 753 1560 1786 2673 3161 3428 3742 4102
Size: 24 807 226 887 488 267 314 360
Cuts at: 4102 5115 5360 729
Size: 1013 245 1686
Fragments arranged by size:
1686 1013 887 807 488 360 314 267
245 226 24
NaeI GCC'GGC
Cuts at: 198 649 1221 2496 4498 198
Size: 451 572 1275 2002 2017
Fragments arranged by size:
2017 2002 1275 572 451
NarI GG'CG_CC
Cuts at: 194 3748 3996 194
Size: 3554 248 2515
Fragments arranged by size:
3554 2515 248
NcoI C'CATG_G
Cuts at: 125 2726 125
Size: 2601 3716
NgoMIV G'CCGG_C
Cuts at: 196 647 1219 2494 4496 196
Size: 451 572 1275 2002 2017
Fragments arranged by size:
2017 2002 1275 572 451
NspI r_CATG'y
Cuts at: 2133 3370 3870 4777 2133
Size: 1237 500 907 3673
Fragments arranged by size:
3673 1237 907 500
PciI A'CATG_T
Cuts at: 4773 4773
Size: 6317
PflMI CCAn_nnn'nTGG
Cuts at: 435 2244 435
Size: 1809 4508
PfoI T'CCnGG_A
Cuts at: 905 2060 2436 2567 2648 3106 905
Size: 1155 376 131 81 458 4116
Fragments arranged by size:
4116 1155 458 376 131 81
PmeI GTTT'AAAC
Cuts at: 3316 3316
Size: 6317
PmlI CAC'GTG
Cuts at: 231 231
Size: 6317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3052 3084 5456 5488 3052
Size: 32 2372 32 3881
Fragments arranged by size:
3881 2372 32 32
PpuMI rG'GwC_Cy
Cuts at: 711 2001 2841 3215 711
Size: 1290 840 374 3813
Fragments arranged by size:
3813 1290 840 374
PspOMI G'GGCC_C
Cuts at: 693 693
Size: 6317
PspXI vC'TCGA_Gb
Cuts at: 2101 2101
Size: 6317
PstI C_TGCA'G
Cuts at: 386 458 791 923 1130 1499 1705 2009 2051
Size: 72 333 132 207 369 206 304 42
Cuts at: 2051 2273 2390 2867 3364 4049 386
Size: 222 117 477 497 685 2654
Fragments arranged by size:
2654 685 497 477 369 333 304 222
207 206 132 117 72 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6317
PvuII CAG'CTG
Cuts at: 753 1560 1786 3161 3742 4102 753
Size: 807 226 1375 581 360 2968
Fragments arranged by size:
2968 1375 807 581 360 226
RsrII CG'GwC_CG
Cuts at: 4512 4512
Size: 6317
SacI G_AGCT'C
Cuts at: 347 587 2060 2237 3331 347
Size: 240 1473 177 1094 3333
Fragments arranged by size:
3333 1473 1094 240 177
SalI G'TCGA_C
Cuts at: 3354 3354
Size: 6317
SapI GCTCTTCn'nnn_
Cuts at: 4340 4550 4340
Size: 210 6107
SbfI CC_TGCA'GG
Cuts at: 386 923 2009 3364 386
Size: 537 1086 1355 3339
Fragments arranged by size:
3339 1355 1086 537
ScaI AGT'ACT
Cuts at: 3295 5834 3295
Size: 2539 3778
SfcI C'TryA_G
Cuts at: 33 382 418 454 787 919 1126 1495 1701
Size: 349 36 36 333 132 207 369 206
Cuts at: 1701 1992 2005 2047 2269 2386 2863 3191 3360
Size: 291 13 42 222 117 477 328 169
Cuts at: 3360 4045 5038 5229 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 477 369 349 333 328
291 222 207 206 191 169 132 117
42 36 36 13
SfoI GGC'GCC
Cuts at: 195 3749 3997 195
Size: 3554 248 2515
Fragments arranged by size:
3554 2515 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6317
SmaI CCC'GGG
Cuts at: 2486 2990 3339 2486
Size: 504 349 5464
Fragments arranged by size:
5464 504 349
SmlI C'TyrA_G
Cuts at: 586 1036 1255 1609 1834 2014 2101 2158 3472
Size: 450 219 354 225 180 87 57 1314
Cuts at: 3472 4387 4879 5141 586
Size: 915 492 262 1762
Fragments arranged by size:
1762 1314 915 492 450 354 262 225
219 180 87 57
SnaBI TAC'GTA
Cuts at: 3309 3309
Size: 6317
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6317
SphI G_CATG'C
Cuts at: 2133 3370 3870 2133
Size: 1237 500 4580
Fragments arranged by size:
4580 1237 500
SrfI GCCC'GGGC
Cuts at: 2486 2486
Size: 6317
StuI AGG'CCT
Cuts at: 2242 2242
Size: 6317
StyI C'CwwG_G
Cuts at: 125 263 368 2243 2726 3451 3805 125
Size: 138 105 1875 483 725 354 2637
Fragments arranged by size:
2637 1875 725 483 354 138 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2954 3153 3900 4033 4492 4701 2954
Size: 199 747 133 459 209 4570
Fragments arranged by size:
4570 747 459 209 199 133
TatI w'GTAC_w
Cuts at: 3293 5832 3293
Size: 2539 3778
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5834 5866 5834
Size: 32 6285
Tth111I GACn'n_nGTC
Cuts at: 4114 4114
Size: 6317
XbaI T'CTAG_A
Cuts at: 1905 3348 1905
Size: 1443 4874
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 576 3812 576
Size: 3236 3081
XhoI C'TCGA_G
Cuts at: 2101 2101
Size: 6317
XmaI C'CCGG_G
Cuts at: 2484 2988 3337 2484
Size: 504 349 5464
Fragments arranged by size:
5464 504 349
XmnI GAAnn'nnTTC
Cuts at: 413 1352 413
Size: 939 5378
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AscI AvrII BaeI BfrBI
BmtI BsiWI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PshAI PsiI PsrI
SacII SanDI SexAI SfiI SgrAI SspI SwaI ZraI