Restriction map for
pF1KSDA1274
(Circular) (Six-base) MAPSORT of: pF1KSDA1274.seq Check: 8275 from: 1 to: 5705
pF1KSDA1274 5705 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 489 2363 489
Size: 1874 3831
AccI GT'mk_AC
Cuts at: 2743 2743
Size: 5705
Acc65I G'GTAC_C
Cuts at: 920 2721 920
Size: 1801 3904
AclI AA'CG_TT
Cuts at: 5545 5545
Size: 5705
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1038 1888 1938 2508 3530 3962 4709 4977 1038
Size: 850 50 570 1022 432 747 268 1766
Fragments arranged by size:
1766 1022 850 747 570 432 268 50
AfeI AGC'GCT
Cuts at: 2312 2455 2312
Size: 143 5562
AflIII A'CryG_T
Cuts at: 365 2480 3057 4161 5138 365
Size: 2115 577 1104 977 932
Fragments arranged by size:
2115 1104 977 932 577
AgeI A'CCGG_T
Cuts at: 2639 4093 2639
Size: 1454 4251
AhdI GACnn_n'nnGTC
Cuts at: 657 718 1372 657
Size: 61 654 4990
Fragments arranged by size:
4990 654 61
AleI CACnn'nnGTG
Cuts at: 1124 1124
Size: 5705
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 268 2020 2551 268
Size: 1752 531 3422
Fragments arranged by size:
3422 1752 531
AlwNI CAG_nnn'CTG
Cuts at: 1150 1376 1481 1540 1973 2049 2089 2531 3010
Size: 226 105 59 433 76 40 442 479
Cuts at: 3010 4577 1150
Size: 1567 2278
Fragments arranged by size:
2278 1567 479 442 433 226 105 76
59 40
ApaI G_GGCC'C
Cuts at: 486 1826 1909 486
Size: 1340 83 4282
Fragments arranged by size:
4282 1340 83
ApaLI G'TGCA_C
Cuts at: 4475 4475
Size: 5705
ApoI r'AATT_y
Cuts at: 2239 2709 5058 5314 2239
Size: 470 2349 256 2630
Fragments arranged by size:
2630 2349 470 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5705
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5705
AvaI C'yCGr_G
Cuts at: 671 715 940 2024 2182 2593 2665 2680 2725
Size: 44 225 1084 158 411 72 15 45
Cuts at: 2725 671
Size: 3651
Fragments arranged by size:
3651 1084 411 225 158 72 45 44
15
BamHI G'GATC_C
Cuts at: 2730 2730
Size: 5705
BanI G'GyrC_C
Cuts at: 169 463 920 1102 1218 1650 2282 2721 3135
Size: 294 457 182 116 432 632 439 414
Cuts at: 3135 3383 3418 169
Size: 248 35 2456
Fragments arranged by size:
2456 632 457 439 432 414 294 248
182 116 35
BanII G_rGCy'C
Cuts at: 486 515 833 1345 1531 1573 1826 1909 2204
Size: 29 318 512 186 42 253 83 295
Cuts at: 2204 2672 2719 3749 486
Size: 468 47 1030 2442
Fragments arranged by size:
2442 1030 512 468 318 295 253 186
83 47 42 29
BbeI G_GCGC'C
Cuts at: 3139 3387 3139
Size: 248 5457
BbsI GAAGACnn'nnnn_
Cuts at: 1044 1944 1044
Size: 900 4805
BbvCI CC'TCA_GC
Cuts at: 2110 2110
Size: 5705
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2076 2110 2656 2690 2076
Size: 34 546 34 5091
Fragments arranged by size:
5091 546 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1339 3603 4364 5705 1339
Size: 2264 761 1341 1339
Fragments arranged by size:
2264 1341 1339 761
BclI T'GATC_A
Cuts at: 305 3225 305
Size: 2920 2785
BglI GCCn_nnn'nGGC
Cuts at: 191 1067 2062 2114 191
Size: 876 995 52 3782
Fragments arranged by size:
3782 995 876 52
BglII A'GATC_T
Cuts at: 3220 5257 3220
Size: 2037 3668
BlpI GC'TnA_GC
Cuts at: 778 1525 1530 1609 2817 778
Size: 747 5 79 1208 3666
Fragments arranged by size:
3666 1208 747 79 5
Bme1580I G_kGCm'C
Cuts at: 466 486 1105 1223 1494 1655 1799 1826 1909
Size: 20 619 118 271 161 144 27 83
Cuts at: 1909 2022 2285 3330 3423 4479 466
Size: 113 263 1045 93 1056 1692
Fragments arranged by size:
1692 1056 1045 619 271 263 161 144
118 113 93 83 27 20
BmrI ACTGGGnnnn_n'
Cuts at: 960 2917 2998 3333 5477 960
Size: 1957 81 335 2144 1188
Fragments arranged by size:
2144 1957 1188 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4072 4072
Size: 5705
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 559 685 757 979 1420 1867 1876 2071
Size: 9 126 72 222 441 447 9 195
Cuts at: 2071 2515 2610 2620 550
Size: 444 95 10 3635
Fragments arranged by size:
3635 447 444 441 222 195 126 95
72 10 9 9
Bpu10I CC'TnA_GC
Cuts at: 395 1499 2110 395
Size: 1104 611 3990
Fragments arranged by size:
3990 1104 611
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 2881 3760 4252 4550 337
Size: 2544 879 492 298 1492
Fragments arranged by size:
2544 1492 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 165 991 1599 1843 2323 5409 165
Size: 826 608 244 480 3086 461
Fragments arranged by size:
3086 826 608 480 461 244
BsaAI yAC'GTr
Cuts at: 311 2697 3058 311
Size: 2386 361 2958
Fragments arranged by size:
2958 2386 361
BsaBI GATnn'nnATC
Cuts at: 6 2368 3219 5256 6
Size: 2362 851 2037 455
Fragments arranged by size:
2362 2037 851 455
BsaHI Gr'CG_yC
Cuts at: 974 1727 2338 3136 3384 4086 5582 974
Size: 753 611 798 248 702 1496 1097
Fragments arranged by size:
1496 1097 798 753 702 611 248
BsaWI w'CCGG_w
Cuts at: 749 1335 2639 2894 3415 4093 4367 4514 4925
Size: 586 1304 255 521 678 274 147 411
Cuts at: 4925 749
Size: 1529
Fragments arranged by size:
1529 1304 678 586 521 411 274 255
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 868 898 952 982 1765 1795 868
Size: 30 54 30 783 30 4778
Fragments arranged by size:
4778 783 54 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 340 892 1749 1786 2044 2209 2212 340
Size: 552 857 37 258 165 3 3833
Fragments arranged by size:
3833 857 552 258 165 37 3
BseYI C'CCAG_C
Cuts at: 149 459 538 773 1003 1041 1139 1344 1679
Size: 310 79 235 230 38 98 205 335
Cuts at: 1679 1684 1862 2583 3130 3888 4465 4961 149
Size: 5 178 721 547 758 577 496 893
Fragments arranged by size:
893 758 721 577 547 496 335 310
235 230 205 178 98 79 38 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 662 2284 2358 523
Size: 139 1622 74 3870
Fragments arranged by size:
3870 1622 139 74
BsiEI CG_ry'CG
Cuts at: 104 1336 3247 3293 4501 104
Size: 1232 1911 46 1208 1308
Fragments arranged by size:
1911 1308 1232 1208 46
BsiHKAI G_wGCw'C
Cuts at: 247 401 1531 2204 2719 3497 3687 4479 247
Size: 154 1130 673 515 778 190 792 1473
Fragments arranged by size:
1473 1130 792 778 673 515 190 154
Bsp1286I G_dGCh'C
Cuts at: 247 401 466 486 515 833 1105 1223 1345
Size: 154 65 20 29 318 272 118 122
Cuts at: 1345 1494 1531 1573 1655 1799 1826 1909 2022
Size: 149 37 42 82 144 27 83 113
Cuts at: 2022 2204 2285 2672 2719 3330 3423 3497 3687
Size: 182 81 387 47 611 93 74 190
Cuts at: 3687 3749 4479 247
Size: 62 730 1473
Fragments arranged by size:
1473 730 611 387 318 272 190 182
154 149 144 122 118 113 93 83
82 81 74 65 62 47 42 37
29 27 20
BspEI T'CCGG_A
Cuts at: 749 4925 749
Size: 4176 1529
BspHI T'CATG_A
Cuts at: 690 1440 4881 690
Size: 750 3441 1514
Fragments arranged by size:
3441 1514 750
BspMI ACCTGCnnnn'nnnn_
Cuts at: 489 1939 2363 2755 2969 3271 3652 489
Size: 1450 424 392 214 302 381 2542
Fragments arranged by size:
2542 1450 424 392 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2606 3997 4051 5535 5700 50
Size: 2556 1391 54 1484 165 55
Fragments arranged by size:
2556 1484 1391 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3617 3617
Size: 5705
BsrFI r'CCGG_y
Cuts at: 374 2639 3703 3884 4093 374
Size: 2265 1064 181 209 1986
Fragments arranged by size:
2265 1986 1064 209 181
BsrGI T'GTAC_A
Cuts at: 884 884
Size: 5705
BssSI C'ACGA_G
Cuts at: 2203 3976 4334 2203
Size: 1773 358 3574
Fragments arranged by size:
3574 1773 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2415 3082 2415
Size: 667 5038
BstBI TT'CG_AA
Cuts at: 110 4066 110
Size: 3956 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 291 709 1698 291
Size: 418 989 4298
Fragments arranged by size:
4298 989 418
BstYI r'GATC_y
Cuts at: 1 335 758 2570 2730 3197 3220 3555 3801
Size: 334 423 1812 160 467 23 335 246
Cuts at: 3801 4813 4899 4911 5257 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1812 1012 467 449 423 346 335 334
246 160 86 23 12
BtgI C'CryG_G
Cuts at: 125 467 849 1029 125
Size: 342 382 180 4801
Fragments arranged by size:
4801 382 342 180
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1545 2152 3655 3836 3999 1545
Size: 607 1503 181 163 3251
Fragments arranged by size:
3251 1503 607 181 163
BtsI GCAGTG_nn'
Cuts at: 201 1131 1262 2948 201
Size: 930 131 1686 2958
Fragments arranged by size:
2958 1686 930 131
DraI TTT'AAA
Cuts at: 2704 2704
Size: 5705
DraIII CAC_nnn'GTG
Cuts at: 2289 2289
Size: 5705
DrdI GACnn_nn'nnGTC
Cuts at: 979 1591 3411 4269 979
Size: 612 1820 858 2415
Fragments arranged by size:
2415 1820 858 612
EaeI y'GGCC_r
Cuts at: 137 743 842 1634 2115 3290 3464 3855 3882
Size: 606 99 792 481 1175 174 391 27
Cuts at: 3882 5227 137
Size: 1345 615
Fragments arranged by size:
1345 1175 792 615 606 481 391 174
99 27
EagI C'GGCC_G
Cuts at: 3290 3290
Size: 5705
EarI CTCTTCn'nnn_
Cuts at: 904 1238 2479 3728 3938 904
Size: 334 1241 1249 210 2671
Fragments arranged by size:
2671 1249 1241 334 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3913 4223 4369 5515 3913
Size: 310 146 1146 4103
Fragments arranged by size:
4103 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1529 2202 2717 1529
Size: 673 515 4517
Fragments arranged by size:
4517 673 515
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 559 685 757 979 1038 1420 1867 1876
Size: 9 126 72 222 59 382 447 9
Cuts at: 1876 1888 1938 2071 2508 2515 2610 2620 3530
Size: 12 50 133 437 7 95 10 910
Cuts at: 3530 3962 4709 4977 550
Size: 432 747 268 1278
Fragments arranged by size:
1278 910 747 447 437 432 382 268
222 133 126 95 72 59 50 12
10 9 9 7
EcoNI CCTnn'n_nnAGG
Cuts at: 1579 1768 1892 2074 2497 2514 2625 1579
Size: 189 124 182 423 17 111 4659
Fragments arranged by size:
4659 423 189 182 124 111 17
EcoO109I rG'GnC_Cy
Cuts at: 183 482 483 561 705 816 1537 1554 1695
Size: 299 1 78 144 111 721 17 141
Cuts at: 1695 1823 1905 1906 1970 2055 2079 2519 2844
Size: 128 82 1 64 85 24 440 325
Cuts at: 2844 183
Size: 3044
Fragments arranged by size:
3044 721 440 325 299 144 141 128
111 85 82 78 64 24 17 1
1
EcoRI G'AATT_C
Cuts at: 2709 2709
Size: 5705
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3724 3724
Size: 5705
FspI TGC'GCA
Cuts at: 3486 3486
Size: 5705
HaeII r_GCGC'y
Cuts at: 1390 1691 2314 2457 3139 3387 4409 5388 1390
Size: 301 623 143 682 248 1022 979 1707
Fragments arranged by size:
1707 1022 979 682 623 301 248 143
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 195 227 643 675 704 736 1358 1390 1579
Size: 32 416 32 29 32 622 32 189
Cuts at: 1579 1611 2533 2565 3547 3577 3579 3609 195
Size: 32 922 32 982 30 2 30 2291
Fragments arranged by size:
2291 982 922 622 416 189 32 32
32 32 32 32 30 30 29 2
HincII GTy'rAC
Cuts at: 2744 2744
Size: 5705
KasI G'GCGC_C
Cuts at: 3135 3383 3135
Size: 248 5457
KpnI G_GTAC'C
Cuts at: 924 2725 924
Size: 1801 3904
MluI A'CGCG_T
Cuts at: 2480 5138 2480
Size: 2658 3047
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 950 1356 1763 4376 4560 950
Size: 406 407 2613 184 2095
Fragments arranged by size:
2613 2095 407 406 184
MscI TGG'CCA
Cuts at: 745 844 1636 3466 745
Size: 99 792 1830 2984
Fragments arranged by size:
2984 1830 792 99
MslI CAynn'nnrTG
Cuts at: 1124 2002 2533 3694 3821 1124
Size: 878 531 1161 127 3008
Fragments arranged by size:
3008 1161 878 531 127
MspA1I CmG'CkG
Cuts at: 777 1058 1455 1481 1845 1866 2046 2105 2114
Size: 281 397 26 364 21 180 59 9
Cuts at: 2114 2268 2430 2816 3130 3490 4503 4748 777
Size: 154 162 386 314 360 1013 245 1734
Fragments arranged by size:
1734 1013 397 386 364 360 314 281
245 180 162 154 59 26 21 9
NaeI GCC'GGC
Cuts at: 376 3886 376
Size: 3510 2195
NarI GG'CG_CC
Cuts at: 3136 3384 3136
Size: 248 5457
NcoI C'CATG_G
Cuts at: 125 849 125
Size: 724 4981
NgoMIV G'CCGG_C
Cuts at: 374 3884 374
Size: 3510 2195
NspI r_CATG'y
Cuts at: 871 1662 2758 3258 4165 871
Size: 791 1096 500 907 2411
Fragments arranged by size:
2411 1096 907 791 500
PasI CC'CwG_GG
Cuts at: 1224 1350 1698 1224
Size: 126 348 5231
Fragments arranged by size:
5231 348 126
PciI A'CATG_T
Cuts at: 4161 4161
Size: 5705
PflMI CCAn_nnn'nTGG
Cuts at: 840 2049 2089 840
Size: 1209 40 4456
Fragments arranged by size:
4456 1209 40
PfoI T'CCnGG_A
Cuts at: 992 992
Size: 5705
PmeI GTTT'AAAC
Cuts at: 2704 2704
Size: 5705
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4844 4876 4844
Size: 32 5673
PpuMI rG'GwC_Cy
Cuts at: 561 816 1554 2079 561
Size: 255 738 525 4187
Fragments arranged by size:
4187 738 525 255
PspOMI G'GGCC_C
Cuts at: 482 1822 1905 482
Size: 1340 83 4282
Fragments arranged by size:
4282 1340 83
PspXI vC'TCGA_Gb
Cuts at: 2665 2665
Size: 5705
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 114 146 114
Size: 32 5673
PstI C_TGCA'G
Cuts at: 908 1016 2429 2444 2662 2752 3437 908
Size: 108 1413 15 218 90 685 3176
Fragments arranged by size:
3176 1413 685 218 108 90 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5705
PvuII CAG'CTG
Cuts at: 777 1058 1845 1866 2046 2105 2268 2430 3130
Size: 281 787 21 180 59 163 162 700
Cuts at: 3130 3490 777
Size: 360 2992
Fragments arranged by size:
2992 787 700 360 281 180 163 162
59 21
RsrII CG'GwC_CG
Cuts at: 3900 3900
Size: 5705
SacI G_AGCT'C
Cuts at: 1531 2204 2719 1531
Size: 673 515 4517
Fragments arranged by size:
4517 673 515
SalI G'TCGA_C
Cuts at: 2742 2742
Size: 5705
SapI GCTCTTCn'nnn_
Cuts at: 3728 3938 3728
Size: 210 5495
SbfI CC_TGCA'GG
Cuts at: 2752 2752
Size: 5705
ScaI AGT'ACT
Cuts at: 2446 5222 2446
Size: 2776 2929
SexAI A'CCwGG_T
Cuts at: 404 404
Size: 5705
SfcI C'TryA_G
Cuts at: 33 187 904 915 1012 1803 1917 2425 2440
Size: 154 717 11 97 791 114 508 15
Cuts at: 2440 2658 2748 3433 4426 4617 33
Size: 218 90 685 993 191 1121
Fragments arranged by size:
1121 993 791 717 685 508 218 191
154 114 97 90 15 11
SfiI GGCCn_nnn'nGGCC
Cuts at: 2062 2062
Size: 5705
SfoI GGC'GCC
Cuts at: 3137 3385 3137
Size: 248 5457
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5705
SmaI CCC'GGG
Cuts at: 717 2727 717
Size: 2010 3695
SmlI C'TyrA_G
Cuts at: 352 671 2665 2860 3775 4267 4529 352
Size: 319 1994 195 915 492 262 1528
Fragments arranged by size:
1994 1528 915 492 319 262 195
SnaBI TAC'GTA
Cuts at: 2697 2697
Size: 5705
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5705
SphI G_CATG'C
Cuts at: 1662 2758 3258 1662
Size: 1096 500 4109
Fragments arranged by size:
4109 1096 500
StyI C'CwwG_G
Cuts at: 125 708 849 1691 2075 2172 2839 3193 125
Size: 583 141 842 384 97 667 354 2637
Fragments arranged by size:
2637 842 667 583 384 354 141 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1746 2179 3288 3421 3880 4089 1746
Size: 433 1109 133 459 209 3362
Fragments arranged by size:
3362 1109 459 433 209 133
TatI w'GTAC_w
Cuts at: 884 2444 5220 884
Size: 1560 2776 1369
Fragments arranged by size:
2776 1560 1369
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5222 5254 5222
Size: 32 5673
Tth111I GACn'n_nGTC
Cuts at: 2320 3502 2320
Size: 1182 4523
XbaI T'CTAG_A
Cuts at: 2736 2736
Size: 5705
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1089 2136 3200 1089
Size: 1047 1064 3594
Fragments arranged by size:
3594 1064 1047
XhoI C'TCGA_G
Cuts at: 671 2665 671
Size: 1994 3711
XmaI C'CCGG_G
Cuts at: 715 2725 715
Size: 2010 3695
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AflII AloI AscI AvrII BaeI BfrBI BmgBI
BmtI BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI SacII SanDI SgrAI SrfI SspI StuI SwaI
XmnI ZraI