Restriction map for
pF1KSDA1257
(Circular) (Six-base) MAPSORT of: pF1KSDA1257.seq Check: 1411 from: 1 to: 4364
pF1KSDA1257 4364 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1402 1402
Size: 4364
Acc65I G'GTAC_C
Cuts at: 1294 1380 1294
Size: 86 4278
AclI AA'CG_TT
Cuts at: 4204 4204
Size: 4364
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 2189 2621 3368 3636 256
Size: 1933 432 747 268 984
Fragments arranged by size:
1933 984 747 432 268
AfeI AGC'GCT
Cuts at: 1254 1254
Size: 4364
AflIII A'CryG_T
Cuts at: 1716 2820 3797 1716
Size: 1104 977 2283
Fragments arranged by size:
2283 1104 977
AgeI A'CCGG_T
Cuts at: 2752 2752
Size: 4364
AhdI GACnn_n'nnGTC
Cuts at: 717 717
Size: 4364
AleI CACnn'nnGTG
Cuts at: 599 599
Size: 4364
AlwNI CAG_nnn'CTG
Cuts at: 1326 1669 3236 1326
Size: 343 1567 2454
Fragments arranged by size:
2454 1567 343
ApaI G_GGCC'C
Cuts at: 255 255
Size: 4364
ApaLI G'TGCA_C
Cuts at: 3134 3134
Size: 4364
ApoI r'AATT_y
Cuts at: 352 672 1368 3717 3973 352
Size: 320 696 2349 256 743
Fragments arranged by size:
2349 743 696 320 256
AseI AT'TA_AT
Cuts at: 20 1087 20
Size: 1067 3297
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
AvaI C'yCGr_G
Cuts at: 1384 1384
Size: 4364
BamHI G'GATC_C
Cuts at: 1389 1389
Size: 4364
BanI G'GyrC_C
Cuts at: 1294 1380 1794 2042 2077 1294
Size: 86 414 248 35 3581
Fragments arranged by size:
3581 414 248 86 35
BanII G_rGCy'C
Cuts at: 176 255 953 1378 2408 176
Size: 79 698 425 1030 2132
Fragments arranged by size:
2132 1030 698 425 79
BbeI G_GCGC'C
Cuts at: 1798 2046 1798
Size: 248 4116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 270 304 270
Size: 34 4330
BciVI GTATCCnnnnn_n'
Cuts at: 2262 3023 4364 2262
Size: 761 1341 2262
Fragments arranged by size:
2262 1341 761
BclI T'GATC_A
Cuts at: 1884 1884
Size: 4364
BglI GCCn_nnn'nGGC
Cuts at: 1347 1347
Size: 4364
BglII A'GATC_T
Cuts at: 1879 3916 1879
Size: 2037 2327
BlpI GC'TnA_GC
Cuts at: 1476 1476
Size: 4364
Bme1580I G_kGCm'C
Cuts at: 255 335 1989 2082 3138 255
Size: 80 1654 93 1056 1481
Fragments arranged by size:
1654 1481 1056 93 80
BmgBI CAC'GTC
Cuts at: 222 330 771 222
Size: 108 441 3815
Fragments arranged by size:
3815 441 108
BmrI ACTGGGnnnn_n'
Cuts at: 1576 1657 1992 4136 1576
Size: 81 335 2144 1804
Fragments arranged by size:
2144 1804 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 195 282 2731 195
Size: 87 2449 1828
Fragments arranged by size:
2449 1828 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 583
Size: 4364
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 711 1540 2419 2911 3209 711
Size: 829 879 492 298 1866
Fragments arranged by size:
1866 879 829 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1145 4068 1145
Size: 2923 1441
BsaAI yAC'GTr
Cuts at: 339 1356 1717 339
Size: 1017 361 2986
Fragments arranged by size:
2986 1017 361
BsaBI GATnn'nnATC
Cuts at: 6 1075 1878 3915 6
Size: 1069 803 2037 455
Fragments arranged by size:
2037 1069 803 455
BsaHI Gr'CG_yC
Cuts at: 1795 2043 2745 4241 1795
Size: 248 702 1496 1918
Fragments arranged by size:
1918 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1553 2074 2752 3026 3173 3584 1553
Size: 521 678 274 147 411 2333
Fragments arranged by size:
2333 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 171 1200 171
Size: 1029 3335
BseYI C'CCAG_C
Cuts at: 1316 1789 2547 3124 3620 1316
Size: 473 758 577 496 2060
Fragments arranged by size:
2060 758 577 496 473
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 300
Size: 4364
BsiEI CG_ry'CG
Cuts at: 104 1906 1952 3160 104
Size: 1802 46 1208 1308
Fragments arranged by size:
1802 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1378 2156 2346 3138 1378
Size: 778 190 792 2604
Fragments arranged by size:
2604 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 176 255 335 953 1378 1989 2082 2156 2346
Size: 79 80 618 425 611 93 74 190
Cuts at: 2346 2408 3138 176
Size: 62 730 1402
Fragments arranged by size:
1402 730 618 611 425 190 93 80
79 74 62
BspEI T'CCGG_A
Cuts at: 3584 3584
Size: 4364
BspHI T'CATG_A
Cuts at: 3540 3540
Size: 4364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1414 1628 1930 2311 1414
Size: 214 302 381 3467
Fragments arranged by size:
3467 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1284 2656 2710 4194 4359 50
Size: 1234 1372 54 1484 165 55
Fragments arranged by size:
1484 1372 1234 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2276 2276
Size: 4364
BsrFI r'CCGG_y
Cuts at: 754 1291 2362 2543 2752 754
Size: 537 1071 181 209 2366
Fragments arranged by size:
2366 1071 537 209 181
BssSI C'ACGA_G
Cuts at: 207 2635 2993 207
Size: 2428 358 1578
Fragments arranged by size:
2428 1578 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1326 1741 1326
Size: 415 3949
BstBI TT'CG_AA
Cuts at: 110 2725 110
Size: 2615 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 366 1053 1269 366
Size: 687 216 3461
Fragments arranged by size:
3461 687 216
BstYI r'GATC_y
Cuts at: 1 1389 1856 1879 2214 2460 3472 3558 3570
Size: 1388 467 23 335 246 1012 86 12
Cuts at: 3570 3916 1
Size: 346 449
Fragments arranged by size:
1388 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 589 589
Size: 4364
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2314 2495 2658 2314
Size: 181 163 4020
Fragments arranged by size:
4020 181 163
BtsI GCAGTG_nn'
Cuts at: 1299 1607 1299
Size: 308 4056
DraI TTT'AAA
Cuts at: 812 1363 812
Size: 551 3813
DrdI GACnn_nn'nnGTC
Cuts at: 2070 2928 2070
Size: 858 3506
EaeI y'GGCC_r
Cuts at: 548 1271 1339 1348 1949 2123 2514 2541 3886
Size: 723 68 9 601 174 391 27 1345
Cuts at: 3886 548
Size: 1026
Fragments arranged by size:
1345 1026 723 601 391 174 68 27
9
EagI C'GGCC_G
Cuts at: 1949 1949
Size: 4364
EarI CTCTTCn'nnn_
Cuts at: 146 860 1208 2387 2597 146
Size: 714 348 1179 210 1913
Fragments arranged by size:
1913 1179 714 348 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 875 2572 2882 3028 4174 875
Size: 1697 310 146 1146 1065
Fragments arranged by size:
1697 1146 1065 310 146
EcoICRI GAG'CTC
Cuts at: 1376 1376
Size: 4364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 583 2189 2621 3368 3636 256
Size: 327 1606 432 747 268 984
Fragments arranged by size:
1606 984 747 432 327 268
EcoO109I rG'GnC_Cy
Cuts at: 156 251 1237 1503 156
Size: 95 986 266 3017
Fragments arranged by size:
3017 986 266 95
EcoRI G'AATT_C
Cuts at: 1368 1368
Size: 4364
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 4364
FspI TGC'GCA
Cuts at: 2145 2145
Size: 4364
HaeII r_GCGC'y
Cuts at: 1256 1798 2046 3068 4047 1256
Size: 542 248 1022 979 1573
Fragments arranged by size:
1573 1022 979 542 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 283 315 703 735 1084 1116 2206 2236 2238
Size: 32 388 32 349 32 1090 30 2
Cuts at: 2238 2268 283
Size: 30 2379
Fragments arranged by size:
2379 1090 388 349 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 525 1403 525
Size: 878 3486
KasI G'GCGC_C
Cuts at: 1794 2042 1794
Size: 248 4116
KpnI G_GTAC'C
Cuts at: 1298 1384 1298
Size: 86 4278
MluI A'CGCG_T
Cuts at: 3797 3797
Size: 4364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 350 3035 3219 350
Size: 2685 184 1495
Fragments arranged by size:
2685 1495 184
MscI TGG'CCA
Cuts at: 550 1341 2125 550
Size: 791 784 2789
Fragments arranged by size:
2789 791 784
MslI CAynn'nnrTG
Cuts at: 599 2353 2480 599
Size: 1754 127 2483
Fragments arranged by size:
2483 1754 127
MspA1I CmG'CkG
Cuts at: 1269 1475 1789 2149 3162 3407 1269
Size: 206 314 360 1013 245 2226
Fragments arranged by size:
2226 1013 360 314 245 206
NaeI GCC'GGC
Cuts at: 756 2545 756
Size: 1789 2575
NarI GG'CG_CC
Cuts at: 1795 2043 1795
Size: 248 4116
NgoMIV G'CCGG_C
Cuts at: 754 2543 754
Size: 1789 2575
NspI r_CATG'y
Cuts at: 139 1417 1917 2824 139
Size: 1278 500 907 1679
Fragments arranged by size:
1679 1278 907 500
PciI A'CATG_T
Cuts at: 2820 2820
Size: 4364
PfoI T'CCnGG_A
Cuts at: 560 560
Size: 4364
PmeI GTTT'AAAC
Cuts at: 1363 1363
Size: 4364
PmlI CAC'GTG
Cuts at: 339 339
Size: 4364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3503 3535 3503
Size: 32 4332
PpuMI rG'GwC_Cy
Cuts at: 156 156
Size: 4364
PshAI GACnn'nnGTC
Cuts at: 1137 1137
Size: 4364
PspOMI G'GGCC_C
Cuts at: 251 251
Size: 4364
PstI C_TGCA'G
Cuts at: 1411 2096 1411
Size: 685 3679
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4364
PvuII CAG'CTG
Cuts at: 1269 1789 2149 1269
Size: 520 360 3484
Fragments arranged by size:
3484 520 360
RsrII CG'GwC_CG
Cuts at: 2559 2559
Size: 4364
SacI G_AGCT'C
Cuts at: 1378 1378
Size: 4364
SalI G'TCGA_C
Cuts at: 1401 1401
Size: 4364
SapI GCTCTTCn'nnn_
Cuts at: 2387 2597 2387
Size: 210 4154
SbfI CC_TGCA'GG
Cuts at: 1411 1411
Size: 4364
ScaI AGT'ACT
Cuts at: 3881 3881
Size: 4364
SfcI C'TryA_G
Cuts at: 33 1407 2092 3085 3276 33
Size: 1374 685 993 191 1121
Fragments arranged by size:
1374 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1347 1347
Size: 4364
SfoI GGC'GCC
Cuts at: 1796 2044 1796
Size: 248 4116
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
SmaI CCC'GGG
Cuts at: 1386 1386
Size: 4364
SmlI C'TyrA_G
Cuts at: 690 1519 2434 2926 3188 690
Size: 829 915 492 262 1866
Fragments arranged by size:
1866 915 829 492 262
SnaBI TAC'GTA
Cuts at: 1356 1356
Size: 4364
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4364
SphI G_CATG'C
Cuts at: 1417 1917 1417
Size: 500 3864
StyI C'CwwG_G
Cuts at: 242 1498 1852 242
Size: 1256 354 2754
Fragments arranged by size:
2754 1256 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 603 932 1947 2080 2539 2748 603
Size: 329 1015 133 459 209 2219
Fragments arranged by size:
2219 1015 459 329 209 133
TatI w'GTAC_w
Cuts at: 3879 3879
Size: 4364
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 356 388 3881 3913 356
Size: 32 3493 32 807
Fragments arranged by size:
3493 807 32 32
Tth111I GACn'n_nGTC
Cuts at: 2161 2161
Size: 4364
XbaI T'CTAG_A
Cuts at: 1395 1395
Size: 4364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 558 1859 558
Size: 1301 3063
XmaI C'CCGG_G
Cuts at: 1384 1384
Size: 4364
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AlfI AloI AscI AvrII BaeI
BbsI BbvCI BfrBI BmtI Bpu10I BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PsiI PspXI PsrI SacII SanDI SexAI SgrAI
SrfI SspI StuI SwaI XhoI XmnI ZraI