Restriction map for
pF1KSDA1252
(Circular) (Six-base) MAPSORT of: pF1KSDA1252.seq Check: 9326 from: 1 to: 4841
pF1KSDA1252 4841 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 188 1067 1879 188
Size: 879 812 3150
Fragments arranged by size:
3150 879 812
Acc65I G'GTAC_C
Cuts at: 1857 1857
Size: 4841
AclI AA'CG_TT
Cuts at: 4681 4681
Size: 4841
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1043 2666 3098 3845 4113 1043
Size: 1623 432 747 268 1771
Fragments arranged by size:
1771 1623 747 432 268
AflII C'TTAA_G
Cuts at: 514 514
Size: 4841
AflIII A'CryG_T
Cuts at: 431 2193 3297 4274 431
Size: 1762 1104 977 998
Fragments arranged by size:
1762 1104 998 977
AgeI A'CCGG_T
Cuts at: 3229 3229
Size: 4841
AleI CACnn'nnGTG
Cuts at: 1358 1358
Size: 4841
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 269 269
Size: 4841
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 687 719 687
Size: 32 4809
AlwNI CAG_nnn'CTG
Cuts at: 486 2146 3713 486
Size: 1660 1567 1614
Fragments arranged by size:
1660 1614 1567
ApaLI G'TGCA_C
Cuts at: 3611 3611
Size: 4841
ApoI r'AATT_y
Cuts at: 1845 4194 4450 1845
Size: 2349 256 2236
Fragments arranged by size:
2349 2236 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4841
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4841
AvaI C'yCGr_G
Cuts at: 1861 1861
Size: 4841
BamHI G'GATC_C
Cuts at: 1486 1866 1486
Size: 380 4461
BanI G'GyrC_C
Cuts at: 1693 1857 2271 2519 2554 1693
Size: 164 414 248 35 3980
Fragments arranged by size:
3980 414 248 164 35
BanII G_rGCy'C
Cuts at: 167 242 493 602 1855 2885 167
Size: 75 251 109 1253 1030 2123
Fragments arranged by size:
2123 1253 1030 251 109 75
BbeI G_GCGC'C
Cuts at: 2275 2523 2275
Size: 248 4593
BbsI GAAGACnn'nnnn_
Cuts at: 297 1684 1749 297
Size: 1387 65 3389
Fragments arranged by size:
3389 1387 65
BciVI GTATCCnnnnn_n'
Cuts at: 1108 2739 3500 4841 1108
Size: 1631 761 1341 1108
Fragments arranged by size:
1631 1341 1108 761
BclI T'GATC_A
Cuts at: 2361 2361
Size: 4841
BglI GCCn_nnn'nGGC
Cuts at: 1036 1339 1036
Size: 303 4538
BglII A'GATC_T
Cuts at: 2356 4393 2356
Size: 2037 2804
BlpI GC'TnA_GC
Cuts at: 1953 1953
Size: 4841
Bme1580I G_kGCm'C
Cuts at: 849 2466 2559 3615 849
Size: 1617 93 1056 2075
Fragments arranged by size:
2075 1617 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1562 2053 2134 2469 4613 1562
Size: 491 81 335 2144 1790
Fragments arranged by size:
2144 1790 491 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 543 1105 3208 543
Size: 562 2103 2176
Fragments arranged by size:
2176 2103 562
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 806 939 1144 806
Size: 133 205 4503
Fragments arranged by size:
4503 205 133
Bpu10I CC'TnA_GC
Cuts at: 1532 1532
Size: 4841
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1399 2017 2896 3388 3686 1399
Size: 618 879 492 298 2554
Fragments arranged by size:
2554 879 618 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4545 4545
Size: 4841
BsaAI yAC'GTr
Cuts at: 1833 2194 1833
Size: 361 4480
BsaBI GATnn'nnATC
Cuts at: 6 2355 4392 6
Size: 2349 2037 455
Fragments arranged by size:
2349 2037 455
BsaHI Gr'CG_yC
Cuts at: 536 2272 2520 3222 4718 536
Size: 1736 248 702 1496 659
Fragments arranged by size:
1736 1496 702 659 248
BsaWI w'CCGG_w
Cuts at: 2030 2551 3229 3503 3650 4061 2030
Size: 521 678 274 147 411 2810
Fragments arranged by size:
2810 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 598 598
Size: 4841
BseYI C'CCAG_C
Cuts at: 2266 3024 3601 4097 2266
Size: 758 577 496 3010
Fragments arranged by size:
3010 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2383 2429 3637 104
Size: 2279 46 1208 1308
Fragments arranged by size:
2279 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 493 602 1130 1855 2633 2823 3615 493
Size: 109 528 725 778 190 792 1719
Fragments arranged by size:
1719 792 778 725 528 190 109
Bsp1286I G_dGCh'C
Cuts at: 167 242 493 602 849 1130 1855 2466 2559
Size: 75 251 109 247 281 725 611 93
Cuts at: 2559 2633 2823 2885 3615 167
Size: 74 190 62 730 1393
Fragments arranged by size:
1393 730 725 611 281 251 247 190
109 93 75 74 62
BspEI T'CCGG_A
Cuts at: 4061 4061
Size: 4841
BspHI T'CATG_A
Cuts at: 1658 4017 1658
Size: 2359 2482
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1891 2105 2407 2788 1891
Size: 214 302 381 3944
Fragments arranged by size:
3944 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3133 3187 4671 4836 50
Size: 3083 54 1484 165 55
Fragments arranged by size:
3083 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 222 944 1475 2753 222
Size: 722 531 1278 2310
Fragments arranged by size:
2310 1278 722 531
BsrFI r'CCGG_y
Cuts at: 2839 3020 3229 2839
Size: 181 209 4451
Fragments arranged by size:
4451 209 181
BsrGI T'GTAC_A
Cuts at: 572 1772 572
Size: 1200 3641
BssSI C'ACGA_G
Cuts at: 3112 3470 3112
Size: 358 4483
BstAPI GCAn_nnn'nTGC
Cuts at: 2218 2218
Size: 4841
BstBI TT'CG_AA
Cuts at: 110 3202 110
Size: 3092 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1186 1689 1810 1186
Size: 503 121 4217
Fragments arranged by size:
4217 503 121
BstYI r'GATC_y
Cuts at: 1 792 1011 1486 1866 2333 2356 2691 2937
Size: 791 219 475 380 467 23 335 246
Cuts at: 2937 3949 4035 4047 4393 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 791 475 467 449 380 346 335
246 219 86 23 12
BstZ17I GTA'TAC
Cuts at: 189 189
Size: 4841
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1284 2791 2972 3135 1284
Size: 1507 181 163 2990
Fragments arranged by size:
2990 1507 181 163
BtsI GCAGTG_nn'
Cuts at: 962 2084 962
Size: 1122 3719
DraI TTT'AAA
Cuts at: 336 1840 336
Size: 1504 3337
DrdI GACnn_nn'nnGTC
Cuts at: 2547 3405 2547
Size: 858 3983
EaeI y'GGCC_r
Cuts at: 1028 2426 2600 2991 3018 4363 1028
Size: 1398 174 391 27 1345 1506
Fragments arranged by size:
1506 1398 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2426 2426
Size: 4841
EarI CTCTTCn'nnn_
Cuts at: 939 2864 3074 939
Size: 1925 210 2706
Fragments arranged by size:
2706 1925 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3049 3359 3505 4651 3049
Size: 310 146 1146 3239
Fragments arranged by size:
3239 1146 310 146
EcoICRI GAG'CTC
Cuts at: 491 600 1853 491
Size: 109 1253 3479
Fragments arranged by size:
3479 1253 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 806 939 1043 1144 2666 3098 3845 4113 806
Size: 133 104 101 1522 432 747 268 1534
Fragments arranged by size:
1534 1522 747 432 268 133 104 101
EcoNI CCTnn'n_nnAGG
Cuts at: 607 607
Size: 4841
EcoO109I rG'GnC_Cy
Cuts at: 900 1980 900
Size: 1080 3761
EcoRI G'AATT_C
Cuts at: 1845 1845
Size: 4841
EcoRV GAT'ATC
Cuts at: 654 654
Size: 4841
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2860 2860
Size: 4841
FspI TGC'GCA
Cuts at: 2622 2622
Size: 4841
HaeII r_GCGC'y
Cuts at: 2275 2523 3545 4524 2275
Size: 248 1022 979 2592
Fragments arranged by size:
2592 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 804 836 1631 1663 2683 2713 2715 2745 804
Size: 32 795 32 1020 30 2 30 2900
Fragments arranged by size:
2900 1020 795 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1068 1725 1880 1068
Size: 657 155 4029
Fragments arranged by size:
4029 657 155
HindIII A'AGCT_T
Cuts at: 467 467
Size: 4841
KasI G'GCGC_C
Cuts at: 2271 2519 2271
Size: 248 4593
KpnI G_GTAC'C
Cuts at: 1861 1861
Size: 4841
MfeI C'AATT_G
Cuts at: 1465 1465
Size: 4841
MluI A'CGCG_T
Cuts at: 4274 4274
Size: 4841
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3512 3696 3512
Size: 184 4657
MscI TGG'CCA
Cuts at: 1030 2602 1030
Size: 1572 3269
MslI CAynn'nnrTG
Cuts at: 769 967 1184 1358 1687 2830 2957 769
Size: 198 217 174 329 1143 127 2653
Fragments arranged by size:
2653 1143 329 217 198 174 127
MspA1I CmG'CkG
Cuts at: 1171 1952 2266 2626 3639 3884 1171
Size: 781 314 360 1013 245 2128
Fragments arranged by size:
2128 1013 781 360 314 245
NaeI GCC'GGC
Cuts at: 3022 3022
Size: 4841
NarI GG'CG_CC
Cuts at: 2272 2520 2272
Size: 248 4593
NgoMIV G'CCGG_C
Cuts at: 3020 3020
Size: 4841
NspI r_CATG'y
Cuts at: 435 1894 2394 3301 435
Size: 1459 500 907 1975
Fragments arranged by size:
1975 1459 907 500
PasI CC'CwG_GG
Cuts at: 480 1794 480
Size: 1314 3527
PciI A'CATG_T
Cuts at: 431 3297 431
Size: 2866 1975
PfoI T'CCnGG_A
Cuts at: 955 955
Size: 4841
PmeI GTTT'AAAC
Cuts at: 1840 1840
Size: 4841
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3980 4012 3980
Size: 32 4809
PpuMI rG'GwC_Cy
Cuts at: 900 900
Size: 4841
PshAI GACnn'nnGTC
Cuts at: 1073 1073
Size: 4841
PstI C_TGCA'G
Cuts at: 1888 2573 1888
Size: 685 4156
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4841
PvuII CAG'CTG
Cuts at: 1171 2266 2626 1171
Size: 1095 360 3386
Fragments arranged by size:
3386 1095 360
RsrII CG'GwC_CG
Cuts at: 3036 3036
Size: 4841
SacI G_AGCT'C
Cuts at: 493 602 1855 493
Size: 109 1253 3479
Fragments arranged by size:
3479 1253 109
SalI G'TCGA_C
Cuts at: 1066 1878 1066
Size: 812 4029
SanDI GG'GwC_CC
Cuts at: 900 900
Size: 4841
SapI GCTCTTCn'nnn_
Cuts at: 939 2864 3074 939
Size: 1925 210 2706
Fragments arranged by size:
2706 1925 210
SbfI CC_TGCA'GG
Cuts at: 1888 1888
Size: 4841
ScaI AGT'ACT
Cuts at: 4358 4358
Size: 4841
SfcI C'TryA_G
Cuts at: 33 1884 2569 3562 3753 33
Size: 1851 685 993 191 1121
Fragments arranged by size:
1851 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2273 2521 2273
Size: 248 4593
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4841
SmaI CCC'GGG
Cuts at: 1863 1863
Size: 4841
SmlI C'TyrA_G
Cuts at: 514 1414 1996 2911 3403 3665 514
Size: 900 582 915 492 262 1690
Fragments arranged by size:
1690 915 900 582 492 262
SnaBI TAC'GTA
Cuts at: 1833 1833
Size: 4841
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4841
SphI G_CATG'C
Cuts at: 1894 2394 1894
Size: 500 4341
StuI AGG'CCT
Cuts at: 157 157
Size: 4841
StyI C'CwwG_G
Cuts at: 410 1975 2329 410
Size: 1565 354 2922
Fragments arranged by size:
2922 1565 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1145 1192 1581 1681 1807 2424 2557 3016 3225
Size: 47 389 100 126 617 133 459 209
Cuts at: 3225 1145
Size: 2761
Fragments arranged by size:
2761 617 459 389 209 133 126 100
47
TatI w'GTAC_w
Cuts at: 521 572 1235 1772 4356 521
Size: 51 663 537 2584 1006
Fragments arranged by size:
2584 1006 663 537 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4358 4390 4358
Size: 32 4809
Tth111I GACn'n_nGTC
Cuts at: 1786 2638 1786
Size: 852 3989
XbaI T'CTAG_A
Cuts at: 1872 1872
Size: 4841
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 320 1704 2336 320
Size: 1384 632 2825
Fragments arranged by size:
2825 1384 632
XmaI C'CCGG_G
Cuts at: 1861 1861
Size: 4841
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI BsaXI BsgI BsiWI
BsmI BsmBI BssHII BstEII Bsu36I BtgI ClaI CspCI
DraIII FseI FspAI HpaI NcoI NdeI NheI NotI
NruI NsiI PacI PflMI PmlI PsiI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SrfI SspI SwaI
XhoI XmnI ZraI