Restriction map for
pF1KSDA1247
(Circular) (Six-base) MAPSORT of: pF1KSDA1247.seq Check: 7364 from: 1 to: 5749
pF1KSDA1247 5749 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 300 300
Size: 5749
AccI GT'mk_AC
Cuts at: 1064 2785 1064
Size: 1721 4028
Acc65I G'GTAC_C
Cuts at: 2763 2763
Size: 5749
AclI AA'CG_TT
Cuts at: 5589 5589
Size: 5749
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1561 1854 2101 3572 4004 4753 5021 1561
Size: 293 247 1471 432 749 268 2289
Fragments arranged by size:
2289 1471 749 432 293 268 247
AfeI AGC'GCT
Cuts at: 973 973
Size: 5749
AflIII A'CryG_T
Cuts at: 1052 2279 2390 2544 3099 4205 5182 1052
Size: 1227 111 154 555 1106 977 1619
Fragments arranged by size:
1619 1227 1106 977 555 154 111
AgeI A'CCGG_T
Cuts at: 4137 4137
Size: 5749
AleI CACnn'nnGTG
Cuts at: 384 1929 384
Size: 1545 4204
AlwNI CAG_nnn'CTG
Cuts at: 3052 4621 3052
Size: 1569 4180
ApaI G_GGCC'C
Cuts at: 133 926 1168 1564 1836 133
Size: 793 242 396 272 4046
Fragments arranged by size:
4046 793 396 272 242
ApaLI G'TGCA_C
Cuts at: 4519 4519
Size: 5749
ApoI r'AATT_y
Cuts at: 946 2443 2751 5102 5358 946
Size: 1497 308 2351 256 1337
Fragments arranged by size:
2351 1497 1337 308 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5749
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5749
AvaI C'yCGr_G
Cuts at: 135 2767 135
Size: 2632 3117
BamHI G'GATC_C
Cuts at: 2772 2772
Size: 5749
BanI G'GyrC_C
Cuts at: 2337 2763 3177 3425 3460 2337
Size: 426 414 248 35 4626
Fragments arranged by size:
4626 426 414 248 35
BanII G_rGCy'C
Cuts at: 133 926 1168 1564 1590 1836 2761 3791 133
Size: 793 242 396 26 246 925 1030 2091
Fragments arranged by size:
2091 1030 925 793 396 246 242 26
BbeI G_GCGC'C
Cuts at: 2341 3181 3429 2341
Size: 840 248 4661
Fragments arranged by size:
4661 840 248
BbvCI CC'TCA_GC
Cuts at: 2563 2563
Size: 5749
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 126 160 126
Size: 34 5715
BciVI GTATCCnnnnn_n'
Cuts at: 3645 4408 5749 3645
Size: 763 1341 3645
Fragments arranged by size:
3645 1341 763
BclI T'GATC_A
Cuts at: 3267 3267
Size: 5749
BglI GCCn_nnn'nGGC
Cuts at: 221 477 1100 221
Size: 256 623 4870
Fragments arranged by size:
4870 623 256
BglII A'GATC_T
Cuts at: 3262 5301 3262
Size: 2039 3710
BlpI GC'TnA_GC
Cuts at: 1663 2859 1663
Size: 1196 4553
Bme1580I G_kGCm'C
Cuts at: 133 926 1168 1564 1607 1836 3372 3465 4523
Size: 793 242 396 43 229 1536 93 1058
Cuts at: 4523 133
Size: 1359
Fragments arranged by size:
1536 1359 1058 793 396 242 229 93
43
BmgBI CAC'GTC
Cuts at: 743 743
Size: 5749
BmrI ACTGGGnnnn_n'
Cuts at: 2362 2716 2959 3040 3375 5521 2362
Size: 354 243 81 335 2146 2590
Fragments arranged by size:
2590 2146 354 335 243 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 480 4114 480
Size: 3634 2115
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1901 2148 946
Size: 955 247 4547
Fragments arranged by size:
4547 955 247
Bpu10I CC'TnA_GC
Cuts at: 2563 2563
Size: 5749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1270 2134 2242 2923 3802 4296 4594 1270
Size: 864 108 681 879 494 298 2425
Fragments arranged by size:
2425 879 864 681 494 298 108
BsaI GGTCTCn'nnnn_
Cuts at: 2682 5453 2682
Size: 2771 2978
BsaAI yAC'GTr
Cuts at: 582 1055 1158 1776 2282 2303 2393 2547 2739
Size: 473 103 618 506 21 90 154 192
Cuts at: 2739 3100 582
Size: 361 3231
Fragments arranged by size:
3231 618 506 473 361 192 154 103
90 21
BsaBI GATnn'nnATC
Cuts at: 6 3261 5300 6
Size: 3255 2039 455
Fragments arranged by size:
3255 2039 455
BsaHI Gr'CG_yC
Cuts at: 1412 1526 1677 2338 3178 3426 4130 5626 1412
Size: 114 151 661 840 248 704 1496 1535
Fragments arranged by size:
1535 1496 840 704 661 248 151 114
BsaWI w'CCGG_w
Cuts at: 533 2936 3457 4137 4411 4558 4969 533
Size: 2403 521 680 274 147 411 1313
Fragments arranged by size:
2403 1313 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 559 589 2146 2176 559
Size: 30 1557 30 4132
Fragments arranged by size:
4132 1557 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 393 456 1435 2580 393
Size: 63 979 1145 3562
Fragments arranged by size:
3562 1145 979 63
BseYI C'CCAG_C
Cuts at: 180 297 1497 1572 1796 2132 2726 3172 3930
Size: 117 1200 75 224 336 594 446 758
Cuts at: 3930 4509 5005 180
Size: 579 496 924
Fragments arranged by size:
1200 924 758 594 579 496 446 336
224 117 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1620 1996 2304 2355 1620
Size: 376 308 51 5014
Fragments arranged by size:
5014 376 308 51
BsiEI CG_ry'CG
Cuts at: 104 1309 3289 3335 4545 104
Size: 1205 1980 46 1210 1308
Fragments arranged by size:
1980 1308 1210 1205 46
BsiHKAI G_wGCw'C
Cuts at: 2761 3539 3729 4523 2761
Size: 778 190 794 3987
Fragments arranged by size:
3987 794 778 190
BsmI GAATG_Cn'
Cuts at: 2508 2508
Size: 5749
BsmBI CGTCTCn'nnnn_
Cuts at: 1003 1027 1003
Size: 24 5725
Bsp1286I G_dGCh'C
Cuts at: 133 926 1168 1564 1590 1607 1836 2761 3372
Size: 793 242 396 26 17 229 925 611
Cuts at: 3372 3465 3539 3729 3791 4523 133
Size: 93 74 190 62 732 1359
Fragments arranged by size:
1359 925 793 732 611 396 242 229
190 93 74 62 26 17
BspEI T'CCGG_A
Cuts at: 4969 4969
Size: 5749
BspHI T'CATG_A
Cuts at: 4925 4925
Size: 5749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 215 300 1266 2797 3011 3313 3694 215
Size: 85 966 1531 214 302 381 2270
Fragments arranged by size:
2270 1531 966 381 302 214 85
BsrBI CCG'CTC
Cuts at: 50 1305 1739 4039 4093 5579 5744 50
Size: 1255 434 2300 54 1486 165 55
Fragments arranged by size:
2300 1486 1255 434 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1238 3659 1238
Size: 2421 3328
BsrFI r'CCGG_y
Cuts at: 415 1180 1308 3745 3926 4137 415
Size: 765 128 2437 181 211 2027
Fragments arranged by size:
2437 2027 765 211 181 128
BsrGI T'GTAC_A
Cuts at: 1976 1976
Size: 5749
BssSI C'ACGA_G
Cuts at: 142 490 1600 2023 4018 4378 142
Size: 348 1110 423 1995 360 1513
Fragments arranged by size:
1995 1513 1110 423 360 348
BstAPI GCAn_nnn'nTGC
Cuts at: 3124 3124
Size: 5749
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5749
BstEII G'GTnAC_C
Cuts at: 1081 2074 1081
Size: 993 4756
BstXI CCAn_nnnn'nTGG
Cuts at: 593 942 1233 1572 1755 2706 593
Size: 349 291 339 183 951 3636
Fragments arranged by size:
3636 951 349 339 291 183
BstYI r'GATC_y
Cuts at: 1 2772 3239 3262 3597 3843 4857 4943 4955
Size: 2771 467 23 335 246 1014 86 12
Cuts at: 4955 5301 1
Size: 346 449
Fragments arranged by size:
2771 1014 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 1065 1065
Size: 5749
Bsu36I CC'TnA_GG
Cuts at: 809 1838 2059 809
Size: 1029 221 4499
Fragments arranged by size:
4499 1029 221
BtgI C'CryG_G
Cuts at: 125 801 1004 1077 1529 125
Size: 676 203 73 452 4345
Fragments arranged by size:
4345 676 452 203 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3697 3878 4041 3697
Size: 181 163 5405
Fragments arranged by size:
5405 181 163
BtsI GCAGTG_nn'
Cuts at: 1519 2513 2593 2990 1519
Size: 994 80 397 4278
Fragments arranged by size:
4278 994 397 80
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1842 1877 1842
Size: 35 5714
DraI TTT'AAA
Cuts at: 2746 2746
Size: 5749
DraIII CAC_nnn'GTG
Cuts at: 647 647
Size: 5749
DrdI GACnn_nn'nnGTC
Cuts at: 987 1144 2527 3453 4313 987
Size: 157 1383 926 860 2423
Fragments arranged by size:
2423 1383 926 860 157
EaeI y'GGCC_r
Cuts at: 1092 1306 1670 1745 1825 2092 2674 3332 3506
Size: 214 364 75 80 267 582 658 174
Cuts at: 3506 3897 3924 5271 1092
Size: 391 27 1347 1570
Fragments arranged by size:
1570 1347 658 582 391 364 267 214
174 80 75 27
EagI C'GGCC_G
Cuts at: 1306 3332 1306
Size: 2026 3723
EarI CTCTTCn'nnn_
Cuts at: 844 1696 2440 2680 3770 3980 844
Size: 852 744 240 1090 210 2613
Fragments arranged by size:
2613 1090 852 744 240 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 241 3955 4267 4413 5559 241
Size: 3714 312 146 1146 431
Fragments arranged by size:
3714 1146 431 312 146
EcoICRI GAG'CTC
Cuts at: 2759 2759
Size: 5749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1561 1854 1901 2101 2148 3572 4004 4753
Size: 615 293 47 200 47 1424 432 749
Cuts at: 4753 5021 946
Size: 268 1674
Fragments arranged by size:
1674 1424 749 615 432 293 268 200
47 47
EcoO109I rG'GnC_Cy
Cuts at: 130 922 1146 1164 1165 1455 1560 1561 1832
Size: 792 224 18 1 290 105 1 271
Cuts at: 1832 1833 2187 2356 2886 130
Size: 1 354 169 530 2993
Fragments arranged by size:
2993 792 530 354 290 271 224 169
105 18 1 1 1
EcoRI G'AATT_C
Cuts at: 946 2751 946
Size: 1805 3944
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3766 3766
Size: 5749
FspI TGC'GCA
Cuts at: 3528 3528
Size: 5749
HaeII r_GCGC'y
Cuts at: 975 2265 2341 2381 3181 3429 4453 5432 975
Size: 1290 76 40 800 248 1024 979 1292
Fragments arranged by size:
1292 1290 1024 979 800 248 76 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1261 1293 3589 3619 3621 3651 1261
Size: 32 2296 30 2 30 3359
Fragments arranged by size:
3359 2296 32 30 30 2
HincII GTy'rAC
Cuts at: 2786 2786
Size: 5749
KasI G'GCGC_C
Cuts at: 2337 3177 3425 2337
Size: 840 248 4661
Fragments arranged by size:
4661 840 248
KpnI G_GTAC'C
Cuts at: 2767 2767
Size: 5749
MluI A'CGCG_T
Cuts at: 5182 5182
Size: 5749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 588 631 704 1619 4420 4604 588
Size: 43 73 915 2801 184 1733
Fragments arranged by size:
2801 1733 915 184 73 43
MscI TGG'CCA
Cuts at: 1747 1827 2676 3508 1747
Size: 80 849 832 3988
Fragments arranged by size:
3988 849 832 80
MslI CAynn'nnrTG
Cuts at: 268 310 382 384 940 955 991 1753 1918
Size: 42 72 2 556 15 36 762 165
Cuts at: 1918 1929 3736 3863 268
Size: 11 1807 127 2154
Fragments arranged by size:
2154 1807 762 556 165 127 72 42
36 15 11 2
MspA1I CmG'CkG
Cuts at: 966 1497 1649 2496 2858 3172 3532 4547 4792
Size: 531 152 847 362 314 360 1015 245
Cuts at: 4792 966
Size: 1923
Fragments arranged by size:
1923 1015 847 531 362 360 314 245
152
NaeI GCC'GGC
Cuts at: 1182 3928 1182
Size: 2746 3003
NarI GG'CG_CC
Cuts at: 2338 3178 3426 2338
Size: 840 248 4661
Fragments arranged by size:
4661 840 248
NcoI C'CATG_G
Cuts at: 125 1004 125
Size: 879 4870
NdeI CA'TA_TG
Cuts at: 1128 1128
Size: 5749
NgoMIV G'CCGG_C
Cuts at: 1180 3926 1180
Size: 2746 3003
NruI TCG'CGA
Cuts at: 4110 4110
Size: 5749
NspI r_CATG'y
Cuts at: 960 1026 2292 2800 3300 4209 960
Size: 66 1266 508 500 909 2500
Fragments arranged by size:
2500 1266 909 508 500 66
PciI A'CATG_T
Cuts at: 4205 4205
Size: 5749
PflMI CCAn_nnn'nTGG
Cuts at: 291 291
Size: 5749
PfoI T'CCnGG_A
Cuts at: 1232 1232
Size: 5749
PmeI GTTT'AAAC
Cuts at: 2746 2746
Size: 5749
PmlI CAC'GTG
Cuts at: 2282 2303 2282
Size: 21 5728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 451 483 4888 4920 451
Size: 32 4405 32 1280
Fragments arranged by size:
4405 1280 32 32
PpuMI rG'GwC_Cy
Cuts at: 1146 1455 1146
Size: 309 5440
PshAI GACnn'nnGTC
Cuts at: 1460 1460
Size: 5749
PsiI TTA'TAA
Cuts at: 635 635
Size: 5749
PspOMI G'GGCC_C
Cuts at: 129 922 1164 1560 1832 129
Size: 793 242 396 272 4046
Fragments arranged by size:
4046 793 396 272 242
PstI C_TGCA'G
Cuts at: 1991 2042 2270 2794 3479 1991
Size: 51 228 524 685 4261
Fragments arranged by size:
4261 685 524 228 51
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5749
PvuII CAG'CTG
Cuts at: 1497 2496 3172 3532 1497
Size: 999 676 360 3714
Fragments arranged by size:
3714 999 676 360
RsrII CG'GwC_CG
Cuts at: 3942 3942
Size: 5749
SacI G_AGCT'C
Cuts at: 2761 2761
Size: 5749
SalI G'TCGA_C
Cuts at: 2784 2784
Size: 5749
SanDI GG'GwC_CC
Cuts at: 1146 1146
Size: 5749
SapI GCTCTTCn'nnn_
Cuts at: 3770 3980 3770
Size: 210 5539
SbfI CC_TGCA'GG
Cuts at: 1991 2794 1991
Size: 803 4946
ScaI AGT'ACT
Cuts at: 1612 2467 5266 1612
Size: 855 2799 2095
Fragments arranged by size:
2799 2095 855
SfcI C'TryA_G
Cuts at: 33 1987 2038 2266 2790 3475 4470 4661 33
Size: 1954 51 228 524 685 995 191 1121
Fragments arranged by size:
1954 1121 995 685 524 228 191 51
SfiI GGCCn_nnn'nGGCC
Cuts at: 1100 1100
Size: 5749
SfoI GGC'GCC
Cuts at: 2339 3179 3427 2339
Size: 840 248 4661
Fragments arranged by size:
4661 840 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5749
SmaI CCC'GGG
Cuts at: 2769 2769
Size: 5749
SmlI C'TyrA_G
Cuts at: 1285 2149 2257 2902 3817 4311 4573 1285
Size: 864 108 645 915 494 262 2461
Fragments arranged by size:
2461 915 864 645 494 262 108
SnaBI TAC'GTA
Cuts at: 2739 2739
Size: 5749
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5749
SphI G_CATG'C
Cuts at: 2292 2800 3300 2292
Size: 508 500 4741
Fragments arranged by size:
4741 508 500
SspI AAT'ATT
Cuts at: 829 829
Size: 5749
StuI AGG'CCT
Cuts at: 1247 1633 1781 1886 1993 2297 1247
Size: 386 148 105 107 304 4699
Fragments arranged by size:
4699 386 304 148 107 105
StyI C'CwwG_G
Cuts at: 125 689 1004 2881 3235 125
Size: 564 315 1877 354 2639
Fragments arranged by size:
2639 1877 564 354 315
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 172 396 1774 2146 2326 3330 3463 3922 4133
Size: 224 1378 372 180 1004 133 459 211
Cuts at: 4133 172
Size: 1788
Fragments arranged by size:
1788 1378 1004 459 372 224 211 180
133
TatI w'GTAC_w
Cuts at: 1610 1703 1976 2465 5264 1610
Size: 93 273 489 2799 2095
Fragments arranged by size:
2799 2095 489 273 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 824 856 2146 2178 2194 2226 5266 5298 824
Size: 32 1290 32 16 32 3040 32 1275
Fragments arranged by size:
3040 1290 1275 32 32 32 32 16
Tth111I GACn'n_nGTC
Cuts at: 1954 3544 1954
Size: 1590 4159
XbaI T'CTAG_A
Cuts at: 2778 2778
Size: 5749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 264 1740 3242 264
Size: 1476 1502 2771
Fragments arranged by size:
2771 1502 1476
XmaI C'CCGG_G
Cuts at: 2767 2767
Size: 5749
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NruI
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BbsI BfrBI BmtI BsiWI BssHII ClaI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NsiI
PacI PasI PspXI PsrI SacII SexAI SgrAI SrfI
SwaI XhoI XmnI ZraI