Restriction map for
pF1KSDA1214
(Circular) (Six-base) MAPSORT of: pF1KSDA1214.seq Check: 3343 from: 1 to: 4327
pF1KSDA1214 4327 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1128 1128
Size: 4327
AatII G_ACGT'C
Cuts at: 1181 1181
Size: 4327
AccI GT'mk_AC
Cuts at: 1363 1363
Size: 4327
Acc65I G'GTAC_C
Cuts at: 1341 1341
Size: 4327
AclI AA'CG_TT
Cuts at: 4167 4167
Size: 4327
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2150 2582 3331 3599 2150
Size: 432 749 268 2878
Fragments arranged by size:
2878 749 432 268
AflIII A'CryG_T
Cuts at: 527 1677 2783 3760 527
Size: 1150 1106 977 1094
Fragments arranged by size:
1150 1106 1094 977
AgeI A'CCGG_T
Cuts at: 2715 2715
Size: 4327
AleI CACnn'nnGTG
Cuts at: 608 1081 608
Size: 473 3854
AlwNI CAG_nnn'CTG
Cuts at: 817 1630 3199 817
Size: 813 1569 1945
Fragments arranged by size:
1945 1569 813
ApaLI G'TGCA_C
Cuts at: 3097 3097
Size: 4327
ApoI r'AATT_y
Cuts at: 1329 3680 3936 1329
Size: 2351 256 1720
Fragments arranged by size:
2351 1720 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4327
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4327
AvaI C'yCGr_G
Cuts at: 289 1345 289
Size: 1056 3271
AvrII C'CTAG_G
Cuts at: 975 975
Size: 4327
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 921 954 921
Size: 33 4294
BamHI G'GATC_C
Cuts at: 875 980 1350 875
Size: 105 370 3852
Fragments arranged by size:
3852 370 105
BanI G'GyrC_C
Cuts at: 1066 1341 1755 2003 2038 1066
Size: 275 414 248 35 3355
Fragments arranged by size:
3355 414 275 248 35
BanII G_rGCy'C
Cuts at: 278 574 1033 1339 2369 278
Size: 296 459 306 1030 2236
Fragments arranged by size:
2236 1030 459 306 296
BbeI G_GCGC'C
Cuts at: 1759 2007 1759
Size: 248 4079
BbsI GAAGACnn'nnnn_
Cuts at: 343 623 343
Size: 280 4047
BbvCI CC'TCA_GC
Cuts at: 546 546
Size: 4327
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 715 749 715
Size: 34 4293
BciVI GTATCCnnnnn_n'
Cuts at: 1143 2223 2986 4327 1143
Size: 1080 763 1341 1143
Fragments arranged by size:
1341 1143 1080 763
BclI T'GATC_A
Cuts at: 1241 1845 1241
Size: 604 3723
BglI GCCn_nnn'nGGC
Cuts at: 301 409 888 301
Size: 108 479 3740
Fragments arranged by size:
3740 479 108
BglII A'GATC_T
Cuts at: 1840 3879 1840
Size: 2039 2288
BlpI GC'TnA_GC
Cuts at: 1437 1437
Size: 4327
Bme1580I G_kGCm'C
Cuts at: 1950 2043 3101 1950
Size: 93 1058 3176
Fragments arranged by size:
3176 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 751 1537 1618 1953 4099 751
Size: 786 81 335 2146 979
Fragments arranged by size:
2146 979 786 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 4327
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 760 760
Size: 4327
Bpu10I CC'TnA_GC
Cuts at: 546 641 546
Size: 95 4232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1501 2380 2874 3172 1501
Size: 879 494 298 2656
Fragments arranged by size:
2656 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 580 4031 580
Size: 3451 876
BsaAI yAC'GTr
Cuts at: 506 1317 1678 506
Size: 811 361 3155
Fragments arranged by size:
3155 811 361
BsaBI GATnn'nnATC
Cuts at: 6 1839 3878 6
Size: 1833 2039 455
Fragments arranged by size:
2039 1833 455
BsaHI Gr'CG_yC
Cuts at: 380 1178 1756 2004 2708 4204 380
Size: 798 578 248 704 1496 503
Fragments arranged by size:
1496 798 704 578 503 248
BsaWI w'CCGG_w
Cuts at: 521 872 1118 1514 2035 2715 2989 3136 3547
Size: 351 246 396 521 680 274 147 411
Cuts at: 3547 521
Size: 1301
Fragments arranged by size:
1301 680 521 411 396 351 274 246
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 226 256 556 586 226
Size: 30 300 30 3967
Fragments arranged by size:
3967 300 30 30
BseYI C'CCAG_C
Cuts at: 1750 2508 3087 3583 1750
Size: 758 579 496 2494
Fragments arranged by size:
2494 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 486 854 310
Size: 176 368 3783
Fragments arranged by size:
3783 368 176
BsiEI CG_ry'CG
Cuts at: 104 420 1867 1913 3123 104
Size: 316 1447 46 1210 1308
Fragments arranged by size:
1447 1308 1210 316 46
BsiHKAI G_wGCw'C
Cuts at: 365 1339 2117 2307 3101 365
Size: 974 778 190 794 1591
Fragments arranged by size:
1591 974 794 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1169 1169
Size: 4327
Bsp1286I G_dGCh'C
Cuts at: 278 365 574 1033 1339 1950 2043 2117 2307
Size: 87 209 459 306 611 93 74 190
Cuts at: 2307 2369 3101 278
Size: 62 732 1504
Fragments arranged by size:
1504 732 611 459 306 209 190 93
87 74 62
BspEI T'CCGG_A
Cuts at: 521 872 1118 3547 521
Size: 351 246 2429 1301
Fragments arranged by size:
2429 1301 351 246
BspHI T'CATG_A
Cuts at: 3503 3503
Size: 4327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1128 1375 1589 1891 2272 1128
Size: 247 214 302 381 3183
Fragments arranged by size:
3183 381 302 247 214
BsrBI CCG'CTC
Cuts at: 50 2617 2671 4157 4322 50
Size: 2567 54 1486 165 55
Fragments arranged by size:
2567 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 137 1256 2237 137
Size: 1119 981 2227
Fragments arranged by size:
2227 1119 981
BsrFI r'CCGG_y
Cuts at: 887 2323 2504 2715 887
Size: 1436 181 211 2499
Fragments arranged by size:
2499 1436 211 181
BsrGI T'GTAC_A
Cuts at: 698 698
Size: 4327
BssSI C'ACGA_G
Cuts at: 2596 2956 2596
Size: 360 3967
BstAPI GCAn_nnn'nTGC
Cuts at: 1702 1702
Size: 4327
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4327
BstYI r'GATC_y
Cuts at: 1 518 875 980 1350 1817 1840 2175 2421
Size: 517 357 105 370 467 23 335 246
Cuts at: 2421 3435 3521 3533 3879 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 517 467 449 370 357 346 335
246 105 86 23 12
BtgI C'CryG_G
Cuts at: 125 221 384 462 551 125
Size: 96 163 78 89 3901
Fragments arranged by size:
3901 163 96 89 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2275 2456 2619 2275
Size: 181 163 3983
Fragments arranged by size:
3983 181 163
BtsI GCAGTG_nn'
Cuts at: 1227 1568 1227
Size: 341 3986
DraI TTT'AAA
Cuts at: 1324 1324
Size: 4327
DrdI GACnn_nn'nnGTC
Cuts at: 1114 2031 2891 1114
Size: 917 860 2550
Fragments arranged by size:
2550 917 860
EaeI y'GGCC_r
Cuts at: 224 401 626 1910 2084 2475 2502 3849 224
Size: 177 225 1284 174 391 27 1347 702
Fragments arranged by size:
1347 1284 702 391 225 177 174 27
EagI C'GGCC_G
Cuts at: 1910 1910
Size: 4327
EarI CTCTTCn'nnn_
Cuts at: 2348 2558 2348
Size: 210 4117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2533 2845 2991 4137 2533
Size: 312 146 1146 2723
Fragments arranged by size:
2723 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1337 1337
Size: 4327
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 760 2150 2582 3331 3599 760
Size: 1390 432 749 268 1488
Fragments arranged by size:
1488 1390 749 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1464 1464
Size: 4327
EcoRI G'AATT_C
Cuts at: 1329 1329
Size: 4327
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2344 2344
Size: 4327
FspI TGC'GCA
Cuts at: 2106 2106
Size: 4327
HaeII r_GCGC'y
Cuts at: 354 1759 2007 3031 4010 354
Size: 1405 248 1024 979 671
Fragments arranged by size:
1405 1024 979 671 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1166 1198 2167 2197 2199 2229 1166
Size: 32 969 30 2 30 3264
Fragments arranged by size:
3264 969 32 30 30 2
HincII GTy'rAC
Cuts at: 915 1364 915
Size: 449 3878
KasI G'GCGC_C
Cuts at: 1755 2003 1755
Size: 248 4079
KpnI G_GTAC'C
Cuts at: 1345 1345
Size: 4327
MluI A'CGCG_T
Cuts at: 527 3760 527
Size: 3233 1094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 599 1239 2998 3182 599
Size: 640 1759 184 1744
Fragments arranged by size:
1759 1744 640 184
MscI TGG'CCA
Cuts at: 403 628 2086 403
Size: 225 1458 2644
Fragments arranged by size:
2644 1458 225
MslI CAynn'nnrTG
Cuts at: 130 595 608 688 1081 1249 2314 2441 130
Size: 465 13 80 393 168 1065 127 2016
Fragments arranged by size:
2016 1065 465 393 168 127 80 13
MspA1I CmG'CkG
Cuts at: 386 464 1213 1436 1750 2110 3125 3370 386
Size: 78 749 223 314 360 1015 245 1343
Fragments arranged by size:
1343 1015 749 360 314 245 223 78
NaeI GCC'GGC
Cuts at: 889 2506 889
Size: 1617 2710
NarI GG'CG_CC
Cuts at: 1756 2004 1756
Size: 248 4079
NcoI C'CATG_G
Cuts at: 125 125
Size: 4327
NgoMIV G'CCGG_C
Cuts at: 887 2504 887
Size: 1617 2710
NruI TCG'CGA
Cuts at: 2688 2688
Size: 4327
NspI r_CATG'y
Cuts at: 1378 1878 2787 1378
Size: 500 909 2918
Fragments arranged by size:
2918 909 500
PciI A'CATG_T
Cuts at: 2783 2783
Size: 4327
PfoI T'CCnGG_A
Cuts at: 1145 1145
Size: 4327
PmeI GTTT'AAAC
Cuts at: 1324 1324
Size: 4327
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3466 3498 3466
Size: 32 4295
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 250 282 250
Size: 32 4295
PstI C_TGCA'G
Cuts at: 157 539 1372 2057 157
Size: 382 833 685 2427
Fragments arranged by size:
2427 833 685 382
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4327
PvuII CAG'CTG
Cuts at: 1213 1750 2110 1213
Size: 537 360 3430
Fragments arranged by size:
3430 537 360
RsrII CG'GwC_CG
Cuts at: 2520 2520
Size: 4327
SacI G_AGCT'C
Cuts at: 1339 1339
Size: 4327
SacII CC_GC'GG
Cuts at: 387 465 387
Size: 78 4249
SalI G'TCGA_C
Cuts at: 1362 1362
Size: 4327
SapI GCTCTTCn'nnn_
Cuts at: 2348 2558 2348
Size: 210 4117
SbfI CC_TGCA'GG
Cuts at: 1372 1372
Size: 4327
ScaI AGT'ACT
Cuts at: 3844 3844
Size: 4327
SfcI C'TryA_G
Cuts at: 33 153 535 1368 2053 3048 3239 33
Size: 120 382 833 685 995 191 1121
Fragments arranged by size:
1121 995 833 685 382 191 120
SfiI GGCCn_nnn'nGGCC
Cuts at: 409 409
Size: 4327
SfoI GGC'GCC
Cuts at: 1757 2005 1757
Size: 248 4079
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4327
SmaI CCC'GGG
Cuts at: 291 1347 291
Size: 1056 3271
SmlI C'TyrA_G
Cuts at: 1480 2395 2889 3151 1480
Size: 915 494 262 2656
Fragments arranged by size:
2656 915 494 262
SnaBI TAC'GTA
Cuts at: 1317 1317
Size: 4327
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4327
SphI G_CATG'C
Cuts at: 1378 1878 1378
Size: 500 3827
StyI C'CwwG_G
Cuts at: 125 491 975 1459 1813 125
Size: 366 484 484 354 2639
Fragments arranged by size:
2639 484 484 366 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1062 1908 2041 2500 2711 1062
Size: 846 133 459 211 2678
Fragments arranged by size:
2678 846 459 211 133
TatI w'GTAC_w
Cuts at: 698 3842 698
Size: 3144 1183
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3844 3876 3844
Size: 32 4295
Tth111I GACn'n_nGTC
Cuts at: 868 2122 868
Size: 1254 3073
XbaI T'CTAG_A
Cuts at: 927 1356 927
Size: 429 3898
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1820 1820
Size: 4327
XmaI C'CCGG_G
Cuts at: 289 1345 289
Size: 1056 3271
XmnI GAAnn'nnTTC
Cuts at: 529 529
Size: 4327
ZraI GAC'GTC
Cuts at: 1179 1179
Size: 4327
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PciI PfoI PmeI PpiI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI ApaI AscI BfrBI
BmgBI BmtI BseRI BsiWI BsmI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI SanDI SexAI SgrAI SrfI SspI StuI SwaI
XhoI