Restriction map for
pF1KSDA1206
(Circular) (Six-base) MAPSORT of: pF1KSDA1206.seq Check: 9544 from: 1 to: 8864
pF1KSDA1206 8864 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 516 1610 516
Size: 1094 7770
AccI GT'mk_AC
Cuts at: 4802 5902 4802
Size: 1100 7764
Acc65I G'GTAC_C
Cuts at: 5880 5880
Size: 8864
AclI AA'CG_TT
Cuts at: 8704 8704
Size: 8864
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1318 6689 7121 7868 8136 1318
Size: 5371 432 747 268 2046
Fragments arranged by size:
5371 2046 747 432 268
AfeI AGC'GCT
Cuts at: 4358 4358
Size: 8864
AflIII A'CryG_T
Cuts at: 2235 6216 7320 8297 2235
Size: 3981 1104 977 2802
Fragments arranged by size:
3981 2802 1104 977
AgeI A'CCGG_T
Cuts at: 2656 7252 2656
Size: 4596 4268
AhdI GACnn_n'nnGTC
Cuts at: 5384 5384
Size: 8864
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4744 5170 4744
Size: 426 8438
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4645 4677 4645
Size: 32 8832
AlwNI CAG_nnn'CTG
Cuts at: 483 510 1278 1293 1671 1697 1716 2045 2448
Size: 27 768 15 378 26 19 329 403
Cuts at: 2448 2516 3975 4671 5025 5790 6169 7736 483
Size: 68 1459 696 354 765 379 1567 1611
Fragments arranged by size:
1611 1567 1459 768 765 696 403 379
378 354 329 68 27 26 19 15
ApaI G_GGCC'C
Cuts at: 258 885 891 1045 1147 1853 2588 2840 2938
Size: 627 6 154 102 706 735 252 98
Cuts at: 2938 3315 3838 4697 258
Size: 377 523 859 4425
Fragments arranged by size:
4425 859 735 706 627 523 377 252
154 102 98 6
ApaLI G'TGCA_C
Cuts at: 3688 4429 7634 3688
Size: 741 3205 4918
Fragments arranged by size:
4918 3205 741
ApoI r'AATT_y
Cuts at: 5868 8217 8473 5868
Size: 2349 256 6259
Fragments arranged by size:
6259 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8864
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8864
AvaI C'yCGr_G
Cuts at: 361 373 630 884 1011 1219 1303 4696 4745
Size: 12 257 254 127 208 84 3393 49
Cuts at: 4745 5564 5884 361
Size: 819 320 3341
Fragments arranged by size:
3393 3341 819 320 257 254 208 127
84 49 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5490 5523 5490
Size: 33 8831
BamHI G'GATC_C
Cuts at: 4106 5889 4106
Size: 1783 7081
BanI G'GyrC_C
Cuts at: 303 554 657 723 1015 1051 1093 1887 2060
Size: 251 103 66 292 36 42 794 173
Cuts at: 2060 2202 2827 2950 3235 3576 4810 5036 5099
Size: 142 625 123 285 341 1234 226 63
Cuts at: 5099 5880 6294 6542 6577 303
Size: 781 414 248 35 2590
Fragments arranged by size:
2590 1234 794 781 625 414 341 292
285 251 248 226 173 142 123 103
66 63 42 36 35
BanII G_rGCy'C
Cuts at: 258 885 891 1045 1147 1853 2408 2588 2651
Size: 627 6 154 102 706 555 180 63
Cuts at: 2651 2698 2771 2840 2938 3191 3315 3354 3457
Size: 47 73 69 98 253 124 39 103
Cuts at: 3457 3477 3838 4697 4985 5626 5669 5878 6908
Size: 20 361 859 288 641 43 209 1030
Cuts at: 6908 258
Size: 2214
Fragments arranged by size:
2214 1030 859 706 641 627 555 361
288 253 209 180 154 124 103 102
98 73 69 63 47 43 39 20
6
BbeI G_GCGC'C
Cuts at: 558 6298 6546 558
Size: 5740 248 2876
Fragments arranged by size:
5740 2876 248
BbsI GAAGACnn'nnnn_
Cuts at: 3483 3724 4904 5383 3483
Size: 241 1180 479 6964
Fragments arranged by size:
6964 1180 479 241
BbvCI CC'TCA_GC
Cuts at: 590 3583 4081 4991 590
Size: 2993 498 910 4463
Fragments arranged by size:
4463 2993 910 498
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3120 3154 3580 3614 3736 3770 3120
Size: 34 426 34 122 34 8214
Fragments arranged by size:
8214 426 122 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1523 3388 6762 7523 8864 1523
Size: 1865 3374 761 1341 1523
Fragments arranged by size:
3374 1865 1523 1341 761
BclI T'GATC_A
Cuts at: 5745 6384 5745
Size: 639 8225
BglI GCCn_nnn'nGGC
Cuts at: 704 1049 1151 1999 2008 2105 4191 704
Size: 345 102 848 9 97 2086 5377
Fragments arranged by size:
5377 2086 848 345 102 97 9
BglII A'GATC_T
Cuts at: 6379 8416 6379
Size: 2037 6827
BlpI GC'TnA_GC
Cuts at: 355 1452 2914 5663 5976 355
Size: 1097 1462 2749 313 3243
Fragments arranged by size:
3243 2749 1462 1097 313
Bme1580I G_kGCm'C
Cuts at: 258 425 448 662 885 891 1018 1045 1054
Size: 167 23 214 223 6 127 27 9
Cuts at: 1054 1147 1349 1853 1928 2065 2417 2588 2596
Size: 93 202 504 75 137 352 171 8
Cuts at: 2596 2840 2938 2953 3238 3315 3692 3838 4433
Size: 244 98 15 285 77 377 146 595
Cuts at: 4433 4697 4813 5041 5230 5763 6489 6582 7638
Size: 264 116 228 189 533 726 93 1056
Cuts at: 7638 258
Size: 1484
Fragments arranged by size:
1484 1056 726 595 533 504 377 352
285 264 244 228 223 214 202 189
171 167 146 137 127 116 98 93
93 77 75 27 23 15 9 8
6
BmgBI CAC'GTC
Cuts at: 1187 1917 1950 2786 3249 4656 1187
Size: 730 33 836 463 1407 5395
Fragments arranged by size:
5395 1407 836 730 463 33
BmrI ACTGGGnnnn_n'
Cuts at: 1545 2404 2843 3185 5536 6076 6157 6492 8636
Size: 859 439 342 2351 540 81 335 2144
Cuts at: 8636 1545
Size: 1773
Fragments arranged by size:
2351 2144 1773 859 540 439 342 335
81
BmtI G_CTAG'C
Cuts at: 4357 5332 4357
Size: 975 7889
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 337 921 4878 5667 7231 337
Size: 584 3957 789 1564 1970
Fragments arranged by size:
3957 1970 1564 789 584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 753 1300 1318 1430 1561 1597 1886 2311 2321
Size: 547 18 112 131 36 289 425 10
Cuts at: 2321 2350 2965 3055 3301 4027 4036 4114 4315
Size: 29 615 90 246 726 9 78 201
Cuts at: 4315 4393 5086 5722 5785 753
Size: 78 693 636 63 3832
Fragments arranged by size:
3832 726 693 636 615 547 425 289
246 201 131 112 90 78 78 63
36 29 18 10 9
Bpu10I CC'TnA_GC
Cuts at: 590 2765 3583 4081 4863 4991 590
Size: 2175 818 498 782 128 4463
Fragments arranged by size:
4463 2175 818 782 498 128
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2862 3601 4873 6040 6919 7411 7709 2862
Size: 739 1272 1167 879 492 298 4017
Fragments arranged by size:
4017 1272 1167 879 739 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 139 761 4012 4025 4459 8568 139
Size: 622 3251 13 434 4109 435
Fragments arranged by size:
4109 3251 622 435 434 13
BsaAI yAC'GTr
Cuts at: 912 954 1857 4428 5856 6217 912
Size: 42 903 2571 1428 361 3559
Fragments arranged by size:
3559 2571 1428 903 361 42
BsaBI GATnn'nnATC
Cuts at: 6 6378 8415 6
Size: 6372 2037 455
Fragments arranged by size:
6372 2037 455
BsaHI Gr'CG_yC
Cuts at: 555 845 1181 4598 5465 6295 6543 7245 8741
Size: 290 336 3417 867 830 248 702 1496
Cuts at: 8741 555
Size: 678
Fragments arranged by size:
3417 1496 867 830 702 678 336 290
248
BsaWI w'CCGG_w
Cuts at: 1526 2656 3048 5190 6053 6574 7252 7526 7673
Size: 1130 392 2142 863 521 678 274 147
Cuts at: 7673 8084 1526
Size: 411 2306
Fragments arranged by size:
2306 2142 1130 863 678 521 411 392
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 835 865 931 961 2602 2632 3028 3058 3330
Size: 30 66 30 1641 30 396 30 272
Cuts at: 3330 3360 4774 4804 4891 4921 4961 4991 5673
Size: 30 1414 30 87 30 40 30 682
Cuts at: 5673 5703 835
Size: 30 3996
Fragments arranged by size:
3996 1641 1414 682 396 272 87 66
40 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 1699 2347 3354 3430 3691 3925 3928 4264
Size: 1497 648 1007 76 261 234 3 336
Cuts at: 4264 4312 5092 5134 5782 202
Size: 48 780 42 648 3284
Fragments arranged by size:
3284 1497 1007 780 648 648 336 261
234 76 48 42 3
BseYI C'CCAG_C
Cuts at: 435 455 1089 1147 1252 1430 2213 2396 2629
Size: 20 634 58 105 178 783 183 233
Cuts at: 2629 2930 2954 3125 3170 3314 4306 4404 6289
Size: 301 24 171 45 144 992 98 1885
Cuts at: 6289 7047 7624 8120 435
Size: 758 577 496 1179
Fragments arranged by size:
1885 1179 992 783 758 634 577 496
301 233 183 178 171 144 105 98
58 45 24 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 1687 2746 3274 3319 3826 3841 3862 4021
Size: 1545 1059 528 45 507 15 21 159
Cuts at: 4021 4981 5166 5350 142
Size: 960 185 184 3656
Fragments arranged by size:
3656 1545 1059 960 528 507 185 184
159 45 21 15
BsiEI CG_ry'CG
Cuts at: 104 672 1140 3699 6406 6452 7660 104
Size: 568 468 2559 2707 46 1208 1308
Fragments arranged by size:
2707 2559 1308 1208 568 468 46
BsiHKAI G_wGCw'C
Cuts at: 1247 2096 2408 3409 3692 3935 4433 4754 5626
Size: 849 312 1001 283 243 498 321 872
Cuts at: 5626 5669 5878 6656 6846 7638 1247
Size: 43 209 778 190 792 2473
Fragments arranged by size:
2473 1001 872 849 792 778 498 321
312 283 243 209 190 43
BsmI GAATG_Cn'
Cuts at: 5415 5415
Size: 8864
Bsp1286I G_dGCh'C
Cuts at: 258 425 448 662 885 891 1018 1045 1054
Size: 167 23 214 223 6 127 27 9
Cuts at: 1054 1147 1247 1349 1853 1928 2065 2096 2408
Size: 93 100 102 504 75 137 31 312
Cuts at: 2408 2417 2588 2596 2651 2698 2771 2840 2938
Size: 9 171 8 55 47 73 69 98
Cuts at: 2938 2953 3191 3238 3315 3354 3409 3457 3477
Size: 15 238 47 77 39 55 48 20
Cuts at: 3477 3692 3838 3935 4433 4697 4754 4813 4985
Size: 215 146 97 498 264 57 59 172
Cuts at: 4985 5041 5230 5626 5669 5763 5878 6489 6582
Size: 56 189 396 43 94 115 611 93
Cuts at: 6582 6656 6846 6908 7638 258
Size: 74 190 62 730 1484
Fragments arranged by size:
1484 730 611 504 498 396 312 264
238 223 215 214 190 189 172 171
167 146 137 127 115 102 100 98
97 94 93 93 77 75 74 73
69 62 59 57 56 55 55 48
47 47 43 39 31 27 23 20
15 9 9 8 6
BspEI T'CCGG_A
Cuts at: 3048 5190 8084 3048
Size: 2142 2894 3828
Fragments arranged by size:
3828 2894 2142
BspHI T'CATG_A
Cuts at: 4061 4391 8040 4061
Size: 330 3649 4885
Fragments arranged by size:
4885 3649 330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 516 1478 1610 2179 4259 5805 5914 6128 6430
Size: 962 132 569 2080 1546 109 214 302
Cuts at: 6430 6811 516
Size: 381 2569
Fragments arranged by size:
2569 2080 1546 962 569 381 302 214
132 109
BsrBI CCG'CTC
Cuts at: 50 851 905 7156 7210 8694 8859 50
Size: 801 54 6251 54 1484 165 55
Fragments arranged by size:
6251 1484 801 165 55 54 54
BsrDI GCAATG_nn'
Cuts at: 1259 3822 6776 1259
Size: 2563 2954 3347
Fragments arranged by size:
3347 2954 2563
BsrFI r'CCGG_y
Cuts at: 1311 2319 2656 2750 3238 4021 5279 6862 7043
Size: 1008 337 94 488 783 1258 1583 181
Cuts at: 7043 7252 1311
Size: 209 2923
Fragments arranged by size:
2923 1583 1258 1008 783 488 337 209
181 94
BsrGI T'GTAC_A
Cuts at: 1385 3950 4547 1385
Size: 2565 597 5702
Fragments arranged by size:
5702 2565 597
BssHII G'CGCG_C
Cuts at: 4140 4140
Size: 8864
BssSI C'ACGA_G
Cuts at: 7135 7493 7135
Size: 358 8506
BstAPI GCAn_nnn'nTGC
Cuts at: 2516 3975 4737 6241 2516
Size: 1459 762 1504 5139
Fragments arranged by size:
5139 1504 1459 762
BstBI TT'CG_AA
Cuts at: 110 7225 110
Size: 7115 1749
BstEII G'GTnAC_C
Cuts at: 4408 4876 4408
Size: 468 8396
BstXI CCAn_nnnn'nTGG
Cuts at: 699 1342 2152 2989 3388 699
Size: 643 810 837 399 6175
Fragments arranged by size:
6175 837 810 643 399
BstYI r'GATC_y
Cuts at: 1 4106 5570 5889 6356 6379 6714 6960 7972
Size: 4105 1464 319 467 23 335 246 1012
Cuts at: 7972 8058 8070 8416 1
Size: 86 12 346 449
Fragments arranged by size:
4105 1464 1012 467 449 346 335 319
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2666 4187 4522 2666
Size: 1521 335 7008
Fragments arranged by size:
7008 1521 335
BtgI C'CryG_G
Cuts at: 380 876 1037 1079 1169 1322 1401 2492 3699
Size: 496 161 42 90 153 79 1091 1207
Cuts at: 3699 3878 4158 4469 4592 5001 380
Size: 179 280 311 123 409 4243
Fragments arranged by size:
4243 1207 1091 496 409 311 280 179
161 153 123 90 79 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 244 5277 5466 6814 6995 7158 244
Size: 5033 189 1348 181 163 1950
Fragments arranged by size:
5033 1950 1348 189 181 163
BtsI GCAGTG_nn'
Cuts at: 238 341 1491 1695 1995 2076 2094 2280 2361
Size: 103 1150 204 300 81 18 186 81
Cuts at: 2361 2514 3133 3409 3920 6107 238
Size: 153 619 276 511 2187 2995
Fragments arranged by size:
2995 2187 1150 619 511 300 276 204
186 153 103 81 81 18
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 227 262 227
Size: 35 8829
DraI TTT'AAA
Cuts at: 5863 5863
Size: 8864
DraIII CAC_nnn'GTG
Cuts at: 1521 2082 2202 5106 1521
Size: 561 120 2904 5279
Fragments arranged by size:
5279 2904 561 120
DrdI GACnn_nn'nnGTC
Cuts at: 6570 7428 6570
Size: 858 8006
EaeI y'GGCC_r
Cuts at: 309 569 669 689 734 749 1137 1266 2321
Size: 260 100 20 45 15 388 129 1055
Cuts at: 2321 2525 2562 2744 2849 3015 3117 3696 4155
Size: 204 37 182 105 166 102 579 459
Cuts at: 4155 4206 6449 6623 7014 7041 8386 309
Size: 51 2243 174 391 27 1345 787
Fragments arranged by size:
2243 1345 1055 787 579 459 391 388
260 204 182 174 166 129 105 102
100 51 45 37 27 20 15
EagI C'GGCC_G
Cuts at: 669 1137 3696 6449 669
Size: 468 2559 2753 3084
Fragments arranged by size:
3084 2753 2559 468
EarI CTCTTCn'nnn_
Cuts at: 352 4273 4561 4924 6887 7097 352
Size: 3921 288 363 1963 210 2119
Fragments arranged by size:
3921 2119 1963 363 288 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 730 843 859 3199 7072 7382 7528 8674 730
Size: 113 16 2340 3873 310 146 1146 920
Fragments arranged by size:
3873 2340 1146 920 310 146 113 16
EcoICRI GAG'CTC
Cuts at: 2406 5624 5667 5876 2406
Size: 3218 43 209 5394
Fragments arranged by size:
5394 3218 209 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 753 1300 1318 1430 1561 1597 1886 2311 2321
Size: 547 18 112 131 36 289 425 10
Cuts at: 2321 2350 2965 3055 3301 4027 4036 4114 4315
Size: 29 615 90 246 726 9 78 201
Cuts at: 4315 4393 5086 5722 5785 6689 7121 7868 8136
Size: 78 693 636 63 904 432 747 268
Cuts at: 8136 753
Size: 1481
Fragments arranged by size:
1481 904 747 726 693 636 615 547
432 425 289 268 246 201 131 112
90 78 78 63 36 29 18 10
9
EcoNI CCTnn'n_nnAGG
Cuts at: 311 991 2669 4521 4525 5767 5812 311
Size: 680 1678 1852 4 1242 45 3363
Fragments arranged by size:
3363 1852 1678 1242 680 45 4
EcoO109I rG'GnC_Cy
Cuts at: 254 594 675 777 833 881 960 984 1143
Size: 340 81 102 56 48 79 24 159
Cuts at: 1143 1144 1441 1587 1849 1850 2191 2585 2901
Size: 1 297 146 262 1 341 394 316
Cuts at: 2901 2934 2935 3074 3311 3324 3595 3651 3835
Size: 33 1 139 237 13 271 56 184
Cuts at: 3835 3972 4230 4397 4588 4693 4829 4959 5352
Size: 137 258 167 191 105 136 130 393
Cuts at: 5352 5360 5772 6003 254
Size: 8 412 231 3115
Fragments arranged by size:
3115 412 394 393 341 340 316 297
271 262 258 237 231 191 184 167
159 146 139 137 136 130 105 102
81 79 56 56 48 33 24 13
8 1 1 1
EcoRI G'AATT_C
Cuts at: 5868 5868
Size: 8864
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6883 6883
Size: 8864
FspI TGC'GCA
Cuts at: 3758 6645 3758
Size: 2887 5977
HaeII r_GCGC'y
Cuts at: 558 1066 1322 2129 4360 4863 6298 6546 7568
Size: 508 256 807 2231 503 1435 248 1022
Cuts at: 7568 8547 558
Size: 979 875
Fragments arranged by size:
2231 1435 1022 979 875 807 508 503
256 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 835 867 2907 2939 4891 4923 5370 5402 6706
Size: 32 2040 32 1952 32 447 32 1304
Cuts at: 6706 6736 6738 6768 835
Size: 30 2 30 2931
Fragments arranged by size:
2931 2040 1952 1304 447 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3426 5903 3426
Size: 2477 6387
KasI G'GCGC_C
Cuts at: 554 6294 6542 554
Size: 5740 248 2876
Fragments arranged by size:
5740 2876 248
KpnI G_GTAC'C
Cuts at: 5884 5884
Size: 8864
MluI A'CGCG_T
Cuts at: 2235 8297 2235
Size: 6062 2802
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 833 2723 4271 4772 5432 7535 7719 833
Size: 1890 1548 501 660 2103 184 1978
Fragments arranged by size:
2103 1978 1890 1548 660 501 184
MscI TGG'CCA
Cuts at: 691 736 2746 2851 3017 3119 4157 6625 691
Size: 45 2010 105 166 102 1038 2468 2930
Fragments arranged by size:
2930 2468 2010 1038 166 105 102 45
MslI CAynn'nnrTG
Cuts at: 1499 2146 3946 6853 6980 1499
Size: 647 1800 2907 127 3383
Fragments arranged by size:
3383 2907 1800 647 127
MspA1I CmG'CkG
Cuts at: 480 747 878 1039 1151 1383 1571 1593 1659
Size: 267 131 161 112 232 188 22 66
Cuts at: 1659 2400 3100 3258 3486 3701 3825 3864 4005
Size: 741 700 158 228 215 124 39 141
Cuts at: 4005 4103 4173 4995 5796 5975 6289 6649 7662
Size: 98 70 822 801 179 314 360 1013
Cuts at: 7662 7907 480
Size: 245 1437
Fragments arranged by size:
1437 1013 822 801 741 700 360 314
267 245 232 228 215 188 179 161
158 141 131 124 112 98 70 66
39 22
NaeI GCC'GGC
Cuts at: 2321 2752 7045 2321
Size: 431 4293 4140
Fragments arranged by size:
4293 4140 431
NarI GG'CG_CC
Cuts at: 555 6295 6543 555
Size: 5740 248 2876
Fragments arranged by size:
5740 2876 248
NcoI C'CATG_G
Cuts at: 1079 2492 4158 4469 1079
Size: 1413 1666 311 5474
Fragments arranged by size:
5474 1666 1413 311
NgoMIV G'CCGG_C
Cuts at: 2319 2750 7043 2319
Size: 431 4293 4140
Fragments arranged by size:
4293 4140 431
NheI G'CTAG_C
Cuts at: 4353 5328 4353
Size: 975 7889
NspI r_CATG'y
Cuts at: 4554 4843 5917 6417 7324 4554
Size: 289 1074 500 907 6094
Fragments arranged by size:
6094 1074 907 500 289
PasI CC'CwG_GG
Cuts at: 1374 1410 2158 2430 3831 5356 1374
Size: 36 748 272 1401 1525 4882
Fragments arranged by size:
4882 1525 1401 748 272 36
PciI A'CATG_T
Cuts at: 7320 7320
Size: 8864
PflMI CCAn_nnn'nTGG
Cuts at: 168 510 1278 1437 1521 2004 2136 2448 3633
Size: 342 768 159 84 483 132 312 1185
Cuts at: 3633 4293 5025 5700 168
Size: 660 732 675 3332
Fragments arranged by size:
3332 1185 768 732 675 660 483 342
312 159 132 84
PfoI T'CCnGG_A
Cuts at: 1784 3032 3632 4115 5671 1784
Size: 1248 600 483 1556 4977
Fragments arranged by size:
4977 1556 1248 600 483
PmeI GTTT'AAAC
Cuts at: 5863 5863
Size: 8864
PmlI CAC'GTG
Cuts at: 1857 1857
Size: 8864
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8003 8035 8003
Size: 32 8832
PpuMI rG'GwC_Cy
Cuts at: 594 675 960 1587 2191 2901 3324 4397 4959
Size: 81 285 627 604 710 423 1073 562
Cuts at: 4959 5352 5360 5772 594
Size: 393 8 412 3686
Fragments arranged by size:
3686 1073 710 627 604 562 423 412
393 285 81 8
PspOMI G'GGCC_C
Cuts at: 254 881 887 1041 1143 1849 2584 2836 2934
Size: 627 6 154 102 706 735 252 98
Cuts at: 2934 3311 3834 4693 254
Size: 377 523 859 4425
Fragments arranged by size:
4425 859 735 706 627 523 377 252
154 102 98 6
PspXI vC'TCGA_Gb
Cuts at: 373 373
Size: 8864
PstI C_TGCA'G
Cuts at: 371 1990 2512 3404 3915 4178 5237 5795 5813
Size: 1619 522 892 511 263 1059 558 18
Cuts at: 5813 5911 6596 371
Size: 98 685 2639
Fragments arranged by size:
2639 1619 1059 892 685 558 522 511
263 98 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8864
PvuII CAG'CTG
Cuts at: 480 747 1383 1571 1659 3825 4005 4995 5796
Size: 267 636 188 88 2166 180 990 801
Cuts at: 5796 6289 6649 480
Size: 493 360 2695
Fragments arranged by size:
2695 2166 990 801 636 493 360 267
188 180 88
RsrII CG'GwC_CG
Cuts at: 7059 7059
Size: 8864
SacI G_AGCT'C
Cuts at: 2408 5626 5669 5878 2408
Size: 3218 43 209 5394
Fragments arranged by size:
5394 3218 209 43
SacII CC_GC'GG
Cuts at: 879 1040 3702 879
Size: 161 2662 6041
Fragments arranged by size:
6041 2662 161
SalI G'TCGA_C
Cuts at: 5901 5901
Size: 8864
SanDI GG'GwC_CC
Cuts at: 5360 5360
Size: 8864
SapI GCTCTTCn'nnn_
Cuts at: 4273 4561 6887 7097 4273
Size: 288 2326 210 6040
Fragments arranged by size:
6040 2326 288 210
SbfI CC_TGCA'GG
Cuts at: 5911 5911
Size: 8864
ScaI AGT'ACT
Cuts at: 8381 8381
Size: 8864
SexAI A'CCwGG_T
Cuts at: 1517 1883 2198 4574 5102 1517
Size: 366 315 2376 528 5279
Fragments arranged by size:
5279 2376 528 366 315
SfcI C'TryA_G
Cuts at: 33 367 1986 2121 2508 3400 3911 4174 5233
Size: 334 1619 135 387 892 511 263 1059
Cuts at: 5233 5791 5809 5907 6592 7585 7776 33
Size: 558 18 98 685 993 191 1121
Fragments arranged by size:
1619 1121 1059 993 892 685 558 511
387 334 263 191 135 98 18
SfoI GGC'GCC
Cuts at: 556 6296 6544 556
Size: 5740 248 2876
Fragments arranged by size:
5740 2876 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8864
SmaI CCC'GGG
Cuts at: 632 886 1013 4698 4747 5566 5886 632
Size: 254 127 3685 49 819 320 3610
Fragments arranged by size:
3685 3610 819 320 254 127 49
SmlI C'TyrA_G
Cuts at: 373 2877 3616 4852 6019 6934 7426 7688 373
Size: 2504 739 1236 1167 915 492 262 1549
Fragments arranged by size:
2504 1549 1236 1167 915 739 492 262
SnaBI TAC'GTA
Cuts at: 5856 5856
Size: 8864
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8864
SphI G_CATG'C
Cuts at: 4843 5917 6417 4843
Size: 1074 500 7290
Fragments arranged by size:
7290 1074 500
SrfI GCCC'GGGC
Cuts at: 886 886
Size: 8864
StuI AGG'CCT
Cuts at: 698 2002 2183 2264 2354 4868 698
Size: 1304 181 81 90 2514 4694
Fragments arranged by size:
4694 2514 1304 181 90 81
StyI C'CwwG_G
Cuts at: 558 1045 1079 2003 2492 3334 4158 4469 5042
Size: 487 34 924 489 842 824 311 573
Cuts at: 5042 5135 5180 5998 6352 558
Size: 93 45 818 354 3070
Fragments arranged by size:
3070 924 842 824 818 573 489 487
354 311 93 45 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1069 1081 1387 2443 2473 2968 3001 3748 3895
Size: 12 306 1056 30 495 33 747 147
Cuts at: 3895 4090 4396 4786 5394 6447 6580 7039 7248
Size: 195 306 390 608 1053 133 459 209
Cuts at: 7248 1069
Size: 2685
Fragments arranged by size:
2685 1056 1053 747 608 495 459 390
306 306 209 195 147 133 33 30
12
TatI w'GTAC_w
Cuts at: 944 1385 3740 3950 4547 8379 944
Size: 441 2355 210 597 3832 1429
Fragments arranged by size:
3832 2355 1429 597 441 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1579 1611 2602 2634 2787 2819 3028 3060 3330
Size: 32 991 32 153 32 209 32 270
Cuts at: 3330 3362 4961 4993 5673 5705 8381 8413 1579
Size: 32 1599 32 680 32 2676 32 2030
Fragments arranged by size:
2676 2030 1599 991 680 270 209 153
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1915 2905 4798 6661 1915
Size: 990 1893 1863 4118
Fragments arranged by size:
4118 1893 1863 990
XbaI T'CTAG_A
Cuts at: 5895 5895
Size: 8864
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 744 777 1086 2535 3006 3570 6359 744
Size: 33 309 1449 471 564 2789 3249
Fragments arranged by size:
3249 2789 1449 564 471 309 33
XhoI C'TCGA_G
Cuts at: 373 373
Size: 8864
XmaI C'CCGG_G
Cuts at: 630 884 1011 4696 4745 5564 5884 630
Size: 254 127 3685 49 819 320 3610
Fragments arranged by size:
3685 3610 819 320 254 127 49
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AleI AscI AvrII BfrBI BsiWI BsmBI
BstZ17I ClaI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NotI NruI NsiI PacI PshAI PsiI PsrI
SfiI SgrAI SspI SwaI XmnI ZraI