Restriction map for
pF1KSDA1205
(Circular) (Six-base) MAPSORT of: pF1KSDA1205.seq Check: 8644 from: 1 to: 6784
pF1KSDA1205 6784 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 908 908
Size: 6784
AccI GT'mk_AC
Cuts at: 3820 3820
Size: 6784
Acc65I G'GTAC_C
Cuts at: 2585 3798 2585
Size: 1213 5571
AclI AA'CG_TT
Cuts at: 6624 6624
Size: 6784
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1348 1792 3088 3373 3711 4607 5039 5788 6056
Size: 444 1296 285 338 896 432 749 268
Cuts at: 6056 1348
Size: 2076
Fragments arranged by size:
2076 1296 896 749 444 432 338 285
268
AflII C'TTAA_G
Cuts at: 1336 1336
Size: 6784
AflIII A'CryG_T
Cuts at: 2905 3395 4134 5240 6217 2905
Size: 490 739 1106 977 3472
Fragments arranged by size:
3472 1106 977 739 490
AgeI A'CCGG_T
Cuts at: 5172 5172
Size: 6784
AhdI GACnn_n'nnGTC
Cuts at: 3129 3129
Size: 6784
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2555 2555
Size: 6784
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 505 537 946 978 505
Size: 32 409 32 6311
Fragments arranged by size:
6311 409 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1408 2221 3172 3270 3630 3764 4087 5656 1408
Size: 813 951 98 360 134 323 1569 2536
Fragments arranged by size:
2536 1569 951 813 360 323 134 98
ApaI G_GGCC'C
Cuts at: 638 671 1223 3106 638
Size: 33 552 1883 4316
Fragments arranged by size:
4316 1883 552 33
ApaLI G'TGCA_C
Cuts at: 5554 5554
Size: 6784
ApoI r'AATT_y
Cuts at: 2500 3786 6137 6393 2500
Size: 1286 2351 256 2891
Fragments arranged by size:
2891 2351 1286 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6784
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6784
AvaI C'yCGr_G
Cuts at: 211 271 313 670 1262 1428 1439 1619 1783
Size: 60 42 357 592 166 11 180 164
Cuts at: 1783 2005 2275 2848 3802 211
Size: 222 270 573 954 3193
Fragments arranged by size:
3193 954 592 573 357 270 222 180
166 164 60 42 11
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 341 374 341
Size: 33 6751
BamHI G'GATC_C
Cuts at: 285 384 1187 3807 285
Size: 99 803 2620 3262
Fragments arranged by size:
3262 2620 803 99
BanI G'GyrC_C
Cuts at: 144 295 339 394 421 429 774 908 1121
Size: 151 44 55 27 8 345 134 213
Cuts at: 1121 1249 1309 1696 2585 3646 3798 4212 4460
Size: 128 60 387 889 1061 152 414 248
Cuts at: 4460 4495 144
Size: 35 2433
Fragments arranged by size:
2433 1061 889 414 387 345 248 213
152 151 134 128 60 55 44 35
27 8
BanII G_rGCy'C
Cuts at: 245 321 638 671 1223 1325 1979 2012 2252
Size: 76 317 33 552 102 654 33 240
Cuts at: 2252 2840 3106 3147 3436 3528 3796 4826 245
Size: 588 266 41 289 92 268 1030 2203
Fragments arranged by size:
2203 1030 654 588 552 317 289 268
266 240 102 92 76 41 33 33
BbeI G_GCGC'C
Cuts at: 299 343 398 433 778 912 1125 1253 1700
Size: 44 55 35 345 134 213 128 447
Cuts at: 1700 3650 4216 4464 299
Size: 1950 566 248 2619
Fragments arranged by size:
2619 1950 566 447 345 248 213 134
128 55 44 35
BbsI GAAGACnn'nnnn_
Cuts at: 1823 2276 1823
Size: 453 6331
BbvCI CC'TCA_GC
Cuts at: 1295 2063 2069 2414 1295
Size: 768 6 345 5665
Fragments arranged by size:
5665 768 345 6
BciVI GTATCCnnnnn_n'
Cuts at: 342 3553 4680 5443 6784 342
Size: 3211 1127 763 1341 342
Fragments arranged by size:
3211 1341 1127 763 342
BclI T'GATC_A
Cuts at: 4302 4302
Size: 6784
BglI GCCn_nnn'nGGC
Cuts at: 419 1167 1705 1942 2230 3110 419
Size: 748 538 237 288 880 4093
Fragments arranged by size:
4093 880 748 538 288 237
BglII A'GATC_T
Cuts at: 4297 6336 4297
Size: 2039 4745
BlpI GC'TnA_GC
Cuts at: 1582 3367 3496 3894 1582
Size: 1785 129 398 4472
Fragments arranged by size:
4472 1785 398 129
Bme1580I G_kGCm'C
Cuts at: 638 671 1223 1312 1358 3106 4407 4500 5558
Size: 33 552 89 46 1748 1301 93 1058
Cuts at: 5558 638
Size: 1864
Fragments arranged by size:
1864 1748 1301 1058 552 93 89 46
33
BmrI ACTGGGnnnn_n'
Cuts at: 1081 1976 2668 3994 4075 4410 6556 1081
Size: 895 692 1326 81 335 2146 1309
Fragments arranged by size:
2146 1326 1309 895 692 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1454 1836 1872 2874 5149 1454
Size: 382 36 1002 2275 3089
Fragments arranged by size:
3089 2275 1002 382 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 343 1023 1219 1723 2041 3161 3292 259
Size: 84 680 196 504 318 1120 131 3751
Fragments arranged by size:
3751 1120 680 504 318 196 131 84
Bpu10I CC'TnA_GC
Cuts at: 1295 2063 2069 2414 2834 1295
Size: 768 6 345 420 5245
Fragments arranged by size:
5245 768 420 345 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1513 2416 3490 3958 4837 5331 5629 865
Size: 648 903 1074 468 879 494 298 2020
Fragments arranged by size:
2020 1074 903 879 648 494 468 298
BsaI GGTCTCn'nnnn_
Cuts at: 1041 1832 2767 6488 1041
Size: 791 935 3721 1337
Fragments arranged by size:
3721 1337 935 791
BsaAI yAC'GTr
Cuts at: 2637 2908 3222 3774 4135 2637
Size: 271 314 552 361 5286
Fragments arranged by size:
5286 552 361 314 271
BsaBI GATnn'nnATC
Cuts at: 6 4296 6335 6
Size: 4290 2039 455
Fragments arranged by size:
4290 2039 455
BsaHI Gr'CG_yC
Cuts at: 296 340 395 430 655 775 909 1122 1250
Size: 44 55 35 225 120 134 213 128
Cuts at: 1250 1573 1697 3177 3647 4213 4461 5165 6661
Size: 323 124 1480 470 566 248 704 1496
Cuts at: 6661 296
Size: 419
Fragments arranged by size:
1496 1480 704 566 470 419 323 248
225 213 134 128 124 120 55 44
35
BsaWI w'CCGG_w
Cuts at: 1675 2508 3212 3438 3971 4492 5172 5446 5593
Size: 833 704 226 533 521 680 274 147
Cuts at: 5593 6004 1675
Size: 411 2455
Fragments arranged by size:
2455 833 704 680 533 521 411 274
226 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 505 535 946 976 1021 1051 1480 1510 2031
Size: 30 411 30 45 30 429 30 521
Cuts at: 2031 2061 505
Size: 30 5228
Fragments arranged by size:
5228 521 429 411 45 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 289 502 529 1312 1489 1780 1794 1848 2038
Size: 213 27 783 177 291 14 54 190
Cuts at: 2038 2051 2228 2260 2365 3108 3525 3682 289
Size: 13 177 32 105 743 417 157 3391
Fragments arranged by size:
3391 783 743 417 291 213 190 177
177 157 105 54 32 27 14 13
BseYI C'CCAG_C
Cuts at: 253 409 595 741 1634 1894 2140 2719 2751
Size: 156 186 146 893 260 246 579 32
Cuts at: 2751 3148 3372 3469 4207 4965 5544 6040 253
Size: 397 224 97 738 758 579 496 997
Fragments arranged by size:
997 893 758 738 579 579 496 397
260 246 224 186 156 146 97 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2717 3376 2717
Size: 659 6125
BsiEI CG_ry'CG
Cuts at: 104 175 4324 4370 5580 104
Size: 71 4149 46 1210 1308
Fragments arranged by size:
4149 1308 1210 71 46
BsiHKAI G_wGCw'C
Cuts at: 2652 3241 3338 3796 4574 4764 5558 2652
Size: 589 97 458 778 190 794 3878
Fragments arranged by size:
3878 794 778 589 458 190 97
BsmBI CGTCTCn'nnnn_
Cuts at: 1780 2267 2314 2335 2815 1780
Size: 487 47 21 480 5749
Fragments arranged by size:
5749 487 480 47 21
Bsp1286I G_dGCh'C
Cuts at: 245 321 638 671 1223 1312 1325 1358 1979
Size: 76 317 33 552 89 13 33 621
Cuts at: 1979 2012 2252 2652 2840 3106 3147 3241 3338
Size: 33 240 400 188 266 41 94 97
Cuts at: 3338 3436 3528 3796 4407 4500 4574 4764 4826
Size: 98 92 268 611 93 74 190 62
Cuts at: 4826 5558 245
Size: 732 1471
Fragments arranged by size:
1471 732 621 611 552 400 317 268
266 240 190 188 98 97 94 93
92 89 76 74 62 41 33 33
33 13
BspEI T'CCGG_A
Cuts at: 1675 2508 3438 6004 1675
Size: 833 930 2566 2455
Fragments arranged by size:
2566 2455 930 833
BspHI T'CATG_A
Cuts at: 5960 5960
Size: 6784
BspMI ACCTGCnnnn'nnnn_
Cuts at: 908 1850 2997 3305 3743 3832 4046 4348 4729
Size: 942 1147 308 438 89 214 302 381
Cuts at: 4729 908
Size: 2963
Fragments arranged by size:
2963 1147 942 438 381 308 302 214
89
BsrBI CCG'CTC
Cuts at: 50 447 1049 1581 2916 2934 2967 3162 5074
Size: 397 602 532 1335 18 33 195 1912
Cuts at: 5074 5128 6614 6779 50
Size: 54 1486 165 55
Fragments arranged by size:
1912 1486 1335 602 532 397 195 165
55 54 33 18
BsrDI GCAATG_nn'
Cuts at: 2001 2920 4694 2001
Size: 919 1774 4091
Fragments arranged by size:
4091 1774 919
BsrFI r'CCGG_y
Cuts at: 813 2356 4780 4961 5172 813
Size: 1543 2424 181 211 2425
Fragments arranged by size:
2425 2424 1543 211 181
BssSI C'ACGA_G
Cuts at: 5053 5413 5053
Size: 360 6424
BstAPI GCAn_nnn'nTGC
Cuts at: 309 3687 4159 309
Size: 3378 472 2934
Fragments arranged by size:
3378 2934 472
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6784
BstXI CCAn_nnnn'nTGG
Cuts at: 2471 2471
Size: 6784
BstYI r'GATC_y
Cuts at: 1 285 384 1187 3807 4274 4297 4632 4878
Size: 284 99 803 2620 467 23 335 246
Cuts at: 4878 5892 5978 5990 6336 1
Size: 1014 86 12 346 449
Fragments arranged by size:
2620 1014 803 467 449 346 335 284
246 99 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 566 2243 2744 566
Size: 1677 501 4606
Fragments arranged by size:
4606 1677 501
BtgI C'CryG_G
Cuts at: 125 231 245 509 536 722 1125 1171 1940
Size: 106 14 264 27 186 403 46 769
Cuts at: 1940 2978 3700 125
Size: 1038 722 3209
Fragments arranged by size:
3209 1038 769 722 403 264 186 106
46 27 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3211 4732 4913 5076 3211
Size: 1521 181 163 4919
Fragments arranged by size:
4919 1521 181 163
BtsI GCAGTG_nn'
Cuts at: 1211 2336 3688 4025 1211
Size: 1125 1352 337 3970
Fragments arranged by size:
3970 1352 1125 337
DraI TTT'AAA
Cuts at: 3781 3781
Size: 6784
DrdI GACnn_nn'nnGTC
Cuts at: 2401 4488 5348 2401
Size: 2087 860 3837
Fragments arranged by size:
3837 2087 860
EaeI y'GGCC_r
Cuts at: 648 1128 1168 2306 2523 2825 2917 2959 3632
Size: 480 40 1138 217 302 92 42 673
Cuts at: 3632 4367 4541 4932 4959 6306 648
Size: 735 174 391 27 1347 1126
Fragments arranged by size:
1347 1138 1126 735 673 480 391 302
217 174 92 42 40 27
EagI C'GGCC_G
Cuts at: 4367 4367
Size: 6784
EarI CTCTTCn'nnn_
Cuts at: 1973 3046 3437 4805 5015 1973
Size: 1073 391 1368 210 3742
Fragments arranged by size:
3742 1368 1073 391 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 278 782 985 994 1180 1851 2066 2078 2384
Size: 504 203 9 186 671 215 12 306
Cuts at: 2384 4990 5302 5448 6594 278
Size: 2606 312 146 1146 468
Fragments arranged by size:
2606 1146 671 504 468 312 306 215
203 186 146 12 9
EcoICRI GAG'CTC
Cuts at: 3794 3794
Size: 6784
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 343 1023 1219 1348 1723 1792 2041 3088
Size: 84 680 196 129 375 69 249 1047
Cuts at: 3088 3161 3292 3373 3711 4607 5039 5788 6056
Size: 73 131 81 338 896 432 749 268
Cuts at: 6056 259
Size: 987
Fragments arranged by size:
1047 987 896 749 680 432 375 338
268 249 196 131 129 84 81 73
69
EcoNI CCTnn'n_nnAGG
Cuts at: 3661 3661
Size: 6784
EcoO109I rG'GnC_Cy
Cuts at: 154 579 635 1105 1181 1203 1219 2487 2748
Size: 425 56 470 76 22 16 1268 261
Cuts at: 2748 3102 3267 3654 3921 154
Size: 354 165 387 267 3017
Fragments arranged by size:
3017 1268 470 425 387 354 267 261
165 76 56 22 16
EcoRI G'AATT_C
Cuts at: 3786 3786
Size: 6784
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4801 4801
Size: 6784
FspI TGC'GCA
Cuts at: 4563 4563
Size: 6784
HaeII r_GCGC'y
Cuts at: 299 343 398 433 596 778 912 1023 1125
Size: 44 55 35 163 182 134 111 102
Cuts at: 1125 1253 1545 1700 2595 3650 4216 4464 5488
Size: 128 292 155 895 1055 566 248 1024
Cuts at: 5488 6467 299
Size: 979 616
Fragments arranged by size:
1055 1024 979 895 616 566 292 248
182 163 155 134 128 111 102 55
44 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 270 302 1021 1053 1384 1416 1480 1512 1707
Size: 32 719 32 331 32 64 32 195
Cuts at: 1707 1739 2363 2395 3115 3147 4624 4654 4656
Size: 32 624 32 720 32 1477 30 2
Cuts at: 4656 4686 270
Size: 30 2368
Fragments arranged by size:
2368 1477 720 719 624 331 195 64
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 729 3821 729
Size: 3092 3692
KasI G'GCGC_C
Cuts at: 295 339 394 429 774 908 1121 1249 1696
Size: 44 55 35 345 134 213 128 447
Cuts at: 1696 3646 4212 4460 295
Size: 1950 566 248 2619
Fragments arranged by size:
2619 1950 566 447 345 248 213 134
128 55 44 35
KpnI G_GTAC'C
Cuts at: 2589 3802 2589
Size: 1213 5571
MluI A'CGCG_T
Cuts at: 6217 6217
Size: 6784
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1298 5455 5639 1298
Size: 4157 184 2443
Fragments arranged by size:
4157 2443 184
MscI TGG'CCA
Cuts at: 2308 3634 4543 2308
Size: 1326 909 4549
Fragments arranged by size:
4549 1326 909
MslI CAynn'nnrTG
Cuts at: 4771 4898 4771
Size: 127 6657
MspA1I CmG'CkG
Cuts at: 135 247 330 538 741 1043 1942 2016 2124
Size: 112 83 208 203 302 899 74 108
Cuts at: 2124 2163 2221 2945 3408 3764 3893 4207 4567
Size: 39 58 724 463 356 129 314 360
Cuts at: 4567 5582 5827 135
Size: 1015 245 1092
Fragments arranged by size:
1092 1015 899 724 463 360 356 314
302 245 208 203 129 112 108 83
74 58 39
NaeI GCC'GGC
Cuts at: 815 2358 4963 815
Size: 1543 2605 2636
Fragments arranged by size:
2636 2605 1543
NarI GG'CG_CC
Cuts at: 296 340 395 430 775 909 1122 1250 1697
Size: 44 55 35 345 134 213 128 447
Cuts at: 1697 3647 4213 4461 296
Size: 1950 566 248 2619
Fragments arranged by size:
2619 1950 566 447 345 248 213 134
128 55 44 35
NcoI C'CATG_G
Cuts at: 125 231 509 125
Size: 106 278 6400
Fragments arranged by size:
6400 278 106
NgoMIV G'CCGG_C
Cuts at: 813 2356 4961 813
Size: 1543 2605 2636
Fragments arranged by size:
2636 2605 1543
NruI TCG'CGA
Cuts at: 5145 5145
Size: 6784
NspI r_CATG'y
Cuts at: 3690 3835 4335 5244 3690
Size: 145 500 909 5230
Fragments arranged by size:
5230 909 500 145
PciI A'CATG_T
Cuts at: 5240 5240
Size: 6784
PflMI CCAn_nnn'nTGG
Cuts at: 237 3270 3630 237
Size: 3033 360 3391
Fragments arranged by size:
3391 3033 360
PfoI T'CCnGG_A
Cuts at: 2384 2609 2384
Size: 225 6559
PmeI GTTT'AAAC
Cuts at: 3781 3781
Size: 6784
PmlI CAC'GTG
Cuts at: 2908 2908
Size: 6784
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 505 537 946 978 5923 5955 505
Size: 32 409 32 4945 32 1334
Fragments arranged by size:
4945 1334 409 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 579 1105 1203 3654 579
Size: 526 98 2451 3709
Fragments arranged by size:
3709 2451 526 98
PspOMI G'GGCC_C
Cuts at: 634 667 1219 3102 634
Size: 33 552 1883 4316
Fragments arranged by size:
4316 1883 552 33
PspXI vC'TCGA_Gb
Cuts at: 211 1428 211
Size: 1217 5567
PstI C_TGCA'G
Cuts at: 209 1631 1713 1847 1950 2498 3383 3829 4514
Size: 1422 82 134 103 548 885 446 685
Cuts at: 4514 209
Size: 2479
Fragments arranged by size:
2479 1422 885 685 548 446 134 103
82
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6784
PvuII CAG'CTG
Cuts at: 135 1043 3408 4207 4567 135
Size: 908 2365 799 360 2352
Fragments arranged by size:
2365 2352 908 799 360
RsrII CG'GwC_CG
Cuts at: 4977 4977
Size: 6784
SacI G_AGCT'C
Cuts at: 3796 3796
Size: 6784
SacII CC_GC'GG
Cuts at: 248 539 1943 248
Size: 291 1404 5089
Fragments arranged by size:
5089 1404 291
SalI G'TCGA_C
Cuts at: 3819 3819
Size: 6784
SanDI GG'GwC_CC
Cuts at: 579 579
Size: 6784
SapI GCTCTTCn'nnn_
Cuts at: 4805 5015 4805
Size: 210 6574
SbfI CC_TGCA'GG
Cuts at: 1847 3383 3829 1847
Size: 1536 446 4802
Fragments arranged by size:
4802 1536 446
ScaI AGT'ACT
Cuts at: 6301 6301
Size: 6784
SexAI A'CCwGG_T
Cuts at: 1031 1031
Size: 6784
SfcI C'TryA_G
Cuts at: 33 205 1627 1709 1843 1946 2494 3089 3379
Size: 172 1422 82 134 103 548 595 290
Cuts at: 3379 3825 4510 5505 5696 33
Size: 446 685 995 191 1121
Fragments arranged by size:
1422 1121 995 685 595 548 446 290
191 172 134 103 82
SfiI GGCCn_nnn'nGGCC
Cuts at: 1167 1167
Size: 6784
SfoI GGC'GCC
Cuts at: 297 341 396 431 776 910 1123 1251 1698
Size: 44 55 35 345 134 213 128 447
Cuts at: 1698 3648 4214 4462 297
Size: 1950 566 248 2619
Fragments arranged by size:
2619 1950 566 447 345 248 213 134
128 55 44 35
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6784
SmaI CCC'GGG
Cuts at: 1264 3804 1264
Size: 2540 4244
SmlI C'TyrA_G
Cuts at: 211 880 1336 1428 1528 1783 2431 3505 3937
Size: 669 456 92 100 255 648 1074 432
Cuts at: 3937 4852 5346 5608 211
Size: 915 494 262 1387
Fragments arranged by size:
1387 1074 915 669 648 494 456 432
262 255 100 92
SnaBI TAC'GTA
Cuts at: 3774 3774
Size: 6784
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6784
SphI G_CATG'C
Cuts at: 3690 3835 4335 3690
Size: 145 500 6139
Fragments arranged by size:
6139 500 145
StuI AGG'CCT
Cuts at: 1625 1625
Size: 6784
StyI C'CwwG_G
Cuts at: 125 231 425 509 584 827 1358 1931 3023
Size: 106 194 84 75 243 531 573 1092
Cuts at: 3023 3916 4270 125
Size: 893 354 2639
Fragments arranged by size:
2639 1092 893 573 531 354 243 194
106 84 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 210 1110 1562 2052 2481 4365 4498 4957 5168
Size: 900 452 490 429 1884 133 459 211
Cuts at: 5168 210
Size: 1826
Fragments arranged by size:
1884 1826 900 490 459 452 429 211
133
TatI w'GTAC_w
Cuts at: 6299 6299
Size: 6784
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2031 2063 6301 6333 2031
Size: 32 4238 32 2482
Fragments arranged by size:
4238 2482 32 32
Tth111I GACn'n_nGTC
Cuts at: 1033 1822 4579 1033
Size: 789 2757 3238
Fragments arranged by size:
3238 2757 789
XbaI T'CTAG_A
Cuts at: 3813 3813
Size: 6784
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2553 4277 2553
Size: 1724 5060
XhoI C'TCGA_G
Cuts at: 211 1428 1783 211
Size: 1217 355 5212
Fragments arranged by size:
5212 1217 355
XmaI C'CCGG_G
Cuts at: 1262 3802 1262
Size: 2540 4244
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AleI AscI AvrII BcgI BfrBI BmgBI
BmtI BsiWI BsmI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NsiI PacI PasI PshAI PsiI
PsrI SgrAI SrfI SspI SwaI XmnI ZraI