Restriction map for
pF1KSDA1184
(Circular) (Six-base) MAPSORT of: pF1KSDA1184.seq Check: 8720 from: 1 to: 4292
pF1KSDA1184 4292 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1018 1018
Size: 4292
AccI GT'mk_AC
Cuts at: 830 1330 830
Size: 500 3792
Acc65I G'GTAC_C
Cuts at: 392 1308 392
Size: 916 3376
AclI AA'CG_TT
Cuts at: 4132 4132
Size: 4292
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 554 766 1279 2117 2549 3296 3564 175
Size: 379 212 513 838 432 747 268 903
Fragments arranged by size:
903 838 747 513 432 379 268 212
AfeI AGC'GCT
Cuts at: 1135 1135
Size: 4292
AflIII A'CryG_T
Cuts at: 1644 2748 3725 1644
Size: 1104 977 2211
Fragments arranged by size:
2211 1104 977
AgeI A'CCGG_T
Cuts at: 2680 2680
Size: 4292
AlwNI CAG_nnn'CTG
Cuts at: 414 1092 1239 1597 3164 414
Size: 678 147 358 1567 1542
Fragments arranged by size:
1567 1542 678 358 147
ApaI G_GGCC'C
Cuts at: 246 795 1204 1233 246
Size: 549 409 29 3305
Fragments arranged by size:
3305 549 409 29
ApaLI G'TGCA_C
Cuts at: 3062 3062
Size: 4292
ApoI r'AATT_y
Cuts at: 1296 3645 3901 1296
Size: 2349 256 1687
Fragments arranged by size:
2349 1687 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4292
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4292
AvaI C'yCGr_G
Cuts at: 239 388 578 1042 1055 1226 1312 239
Size: 149 190 464 13 171 86 3219
Fragments arranged by size:
3219 464 190 171 149 86 13
BamHI G'GATC_C
Cuts at: 1317 1317
Size: 4292
BanI G'GyrC_C
Cuts at: 392 686 1308 1722 1970 2005 392
Size: 294 622 414 248 35 2679
Fragments arranged by size:
2679 622 414 294 248 35
BanII G_rGCy'C
Cuts at: 246 426 715 795 851 946 1043 1204 1233
Size: 180 289 80 56 95 97 161 29
Cuts at: 1233 1250 1306 2336 246
Size: 17 56 1030 2202
Fragments arranged by size:
2202 1030 289 180 161 97 95 80
56 56 29 17
BbeI G_GCGC'C
Cuts at: 1726 1974 1726
Size: 248 4044
BbvCI CC'TCA_GC
Cuts at: 676 676
Size: 4292
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 369 403 369
Size: 34 4258
BciVI GTATCCnnnnn_n'
Cuts at: 2190 2951 4292 2190
Size: 761 1341 2190
Fragments arranged by size:
2190 1341 761
BclI T'GATC_A
Cuts at: 1812 1812
Size: 4292
BglI GCCn_nnn'nGGC
Cuts at: 550 550
Size: 4292
BglII A'GATC_T
Cuts at: 1807 3844 1807
Size: 2037 2255
BlpI GC'TnA_GC
Cuts at: 940 1404 940
Size: 464 3828
Bme1580I G_kGCm'C
Cuts at: 246 795 1108 1114 1204 1233 1917 2010 3066
Size: 549 313 6 90 29 684 93 1056
Cuts at: 3066 246
Size: 1472
Fragments arranged by size:
1472 1056 684 549 313 93 90 29
6
BmrI ACTGGGnnnn_n'
Cuts at: 657 1217 1504 1585 1920 4064 657
Size: 560 287 81 335 2144 885
Fragments arranged by size:
2144 885 560 335 287 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1152 2659 1152
Size: 1507 2785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 1114
Size: 4292
Bpu10I CC'TnA_GC
Cuts at: 676 676
Size: 4292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1468 2347 2839 3137 1468
Size: 879 492 298 2623
Fragments arranged by size:
2623 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 925 3996 925
Size: 3071 1221
BsaAI yAC'GTr
Cuts at: 1109 1284 1645 1109
Size: 175 361 3756
Fragments arranged by size:
3756 361 175
BsaBI GATnn'nnATC
Cuts at: 6 1806 3843 6
Size: 1800 2037 455
Fragments arranged by size:
2037 1800 455
BsaHI Gr'CG_yC
Cuts at: 1723 1971 2673 4169 1723
Size: 248 702 1496 1846
Fragments arranged by size:
1846 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1481 2002 2680 2954 3101 3512 1481
Size: 521 678 274 147 411 2261
Fragments arranged by size:
2261 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 470 500 470
Size: 30 4262
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1024 1144 1024
Size: 120 4172
BseYI C'CCAG_C
Cuts at: 458 545 960 1232 1717 2475 3052 3548 458
Size: 87 415 272 485 758 577 496 1202
Fragments arranged by size:
1202 758 577 496 485 415 272 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 1099 607
Size: 492 3800
BsiEI CG_ry'CG
Cuts at: 104 1834 1880 3088 104
Size: 1730 46 1208 1308
Fragments arranged by size:
1730 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 946 1250 1306 2084 2274 3066 946
Size: 304 56 778 190 792 2172
Fragments arranged by size:
2172 792 778 304 190 56
Bsp1286I G_dGCh'C
Cuts at: 246 426 715 795 851 946 1043 1108 1114
Size: 180 289 80 56 95 97 65 6
Cuts at: 1114 1204 1233 1250 1306 1917 2010 2084 2274
Size: 90 29 17 56 611 93 74 190
Cuts at: 2274 2336 3066 246
Size: 62 730 1472
Fragments arranged by size:
1472 730 611 289 190 180 97 95
93 90 80 74 65 62 56 56
29 17 6
BspEI T'CCGG_A
Cuts at: 3512 3512
Size: 4292
BspHI T'CATG_A
Cuts at: 996 3468 996
Size: 2472 1820
BspMI ACCTGCnnnn'nnnn_
Cuts at: 893 1018 1342 1556 1858 2239 893
Size: 125 324 214 302 381 2946
Fragments arranged by size:
2946 381 324 302 214 125
BsrBI CCG'CTC
Cuts at: 50 2584 2638 4122 4287 50
Size: 2534 54 1484 165 55
Fragments arranged by size:
2534 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 930 2204 930
Size: 1274 3018
BsrFI r'CCGG_y
Cuts at: 680 1253 2290 2471 2680 680
Size: 573 1037 181 209 2292
Fragments arranged by size:
2292 1037 573 209 181
BsrGI T'GTAC_A
Cuts at: 371 371
Size: 4292
BssSI C'ACGA_G
Cuts at: 2563 2921 2563
Size: 358 3934
BstAPI GCAn_nnn'nTGC
Cuts at: 1077 1669 1077
Size: 592 3700
BstBI TT'CG_AA
Cuts at: 110 2653 110
Size: 2543 1749
BstEII G'GTnAC_C
Cuts at: 612 612
Size: 4292
BstYI r'GATC_y
Cuts at: 1 785 1317 1784 1807 2142 2388 3400 3486
Size: 784 532 467 23 335 246 1012 86
Cuts at: 3486 3498 3844 1
Size: 12 346 449
Fragments arranged by size:
1012 784 532 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 716 716
Size: 4292
BtgI C'CryG_G
Cuts at: 125 125
Size: 4292
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2242 2423 2586 2242
Size: 181 163 3948
Fragments arranged by size:
3948 181 163
BtsI GCAGTG_nn'
Cuts at: 1078 1161 1535 1078
Size: 83 374 3835
Fragments arranged by size:
3835 374 83
DraI TTT'AAA
Cuts at: 1291 1291
Size: 4292
DrdI GACnn_nn'nnGTC
Cuts at: 1998 2856 1998
Size: 858 3434
EaeI y'GGCC_r
Cuts at: 807 1877 2051 2442 2469 3814 807
Size: 1070 174 391 27 1345 1285
Fragments arranged by size:
1345 1285 1070 391 174 27
EagI C'GGCC_G
Cuts at: 1877 1877
Size: 4292
EarI CTCTTCn'nnn_
Cuts at: 403 475 877 895 1238 2315 2525 403
Size: 72 402 18 343 1077 210 2170
Fragments arranged by size:
2170 1077 402 343 210 72 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 924 1240 2500 2810 2956 4102 924
Size: 316 1260 310 146 1146 1114
Fragments arranged by size:
1260 1146 1114 316 310 146
EcoICRI GAG'CTC
Cuts at: 944 1248 1304 944
Size: 304 56 3932
Fragments arranged by size:
3932 304 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 554 766 1114 1279 2117 2549 3296 3564
Size: 379 212 348 165 838 432 747 268
Cuts at: 3564 175
Size: 903
Fragments arranged by size:
903 838 747 432 379 348 268 212
165
EcoNI CCTnn'n_nnAGG
Cuts at: 1126 1126
Size: 4292
EcoO109I rG'GnC_Cy
Cuts at: 236 243 792 859 882 1201 1431 236
Size: 7 549 67 23 319 230 3097
Fragments arranged by size:
3097 549 319 230 67 23 7
EcoRI G'AATT_C
Cuts at: 1296 1296
Size: 4292
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2311 2311
Size: 4292
FspI TGC'GCA
Cuts at: 418 2073 418
Size: 1655 2637
HaeII r_GCGC'y
Cuts at: 154 1137 1174 1726 1974 2996 3975 154
Size: 983 37 552 248 1022 979 471
Fragments arranged by size:
1022 983 979 552 471 248 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1207 1239 2134 2164 2166 2196 1207
Size: 32 895 30 2 30 3303
Fragments arranged by size:
3303 895 32 30 30 2
HincII GTy'rAC
Cuts at: 1331 1331
Size: 4292
KasI G'GCGC_C
Cuts at: 1722 1970 1722
Size: 248 4044
KpnI G_GTAC'C
Cuts at: 396 1312 396
Size: 916 3376
MluI A'CGCG_T
Cuts at: 3725 3725
Size: 4292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2963 3147 2963
Size: 184 4108
MscI TGG'CCA
Cuts at: 809 2053 809
Size: 1244 3048
MslI CAynn'nnrTG
Cuts at: 758 1001 2281 2408 758
Size: 243 1280 127 2642
Fragments arranged by size:
2642 1280 243 127
MspA1I CmG'CkG
Cuts at: 414 549 1083 1089 1403 1717 2077 3090 3335
Size: 135 534 6 314 314 360 1013 245
Cuts at: 3335 414
Size: 1371
Fragments arranged by size:
1371 1013 534 360 314 314 245 135
6
NaeI GCC'GGC
Cuts at: 682 1255 2473 682
Size: 573 1218 2501
Fragments arranged by size:
2501 1218 573
NarI GG'CG_CC
Cuts at: 1723 1971 1723
Size: 248 4044
NcoI C'CATG_G
Cuts at: 125 125
Size: 4292
NgoMIV G'CCGG_C
Cuts at: 680 1253 2471 680
Size: 573 1218 2501
Fragments arranged by size:
2501 1218 573
NspI r_CATG'y
Cuts at: 1345 1845 2752 1345
Size: 500 907 2885
Fragments arranged by size:
2885 907 500
PasI CC'CwG_GG
Cuts at: 1122 1122
Size: 4292
PciI A'CATG_T
Cuts at: 2748 2748
Size: 4292
PflMI CCAn_nnn'nTGG
Cuts at: 1167 1239 1167
Size: 72 4220
PfoI T'CCnGG_A
Cuts at: 1238 1238
Size: 4292
PmeI GTTT'AAAC
Cuts at: 1291 1291
Size: 4292
PmlI CAC'GTG
Cuts at: 1109 1109
Size: 4292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3431 3463 3431
Size: 32 4260
PpuMI rG'GwC_Cy
Cuts at: 236 859 882 236
Size: 623 23 3646
Fragments arranged by size:
3646 623 23
PspOMI G'GGCC_C
Cuts at: 242 791 1200 1229 242
Size: 549 409 29 3305
Fragments arranged by size:
3305 549 409 29
PstI C_TGCA'G
Cuts at: 1339 2024 1339
Size: 685 3607
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4292
PvuII CAG'CTG
Cuts at: 414 549 1717 2077 414
Size: 135 1168 360 2629
Fragments arranged by size:
2629 1168 360 135
RsrII CG'GwC_CG
Cuts at: 2487 2487
Size: 4292
SacI G_AGCT'C
Cuts at: 946 1250 1306 946
Size: 304 56 3932
Fragments arranged by size:
3932 304 56
SalI G'TCGA_C
Cuts at: 1329 1329
Size: 4292
SanDI GG'GwC_CC
Cuts at: 859 859
Size: 4292
SapI GCTCTTCn'nnn_
Cuts at: 895 1238 2315 2525 895
Size: 343 1077 210 2662
Fragments arranged by size:
2662 1077 343 210
SbfI CC_TGCA'GG
Cuts at: 1339 1339
Size: 4292
ScaI AGT'ACT
Cuts at: 3809 3809
Size: 4292
SfcI C'TryA_G
Cuts at: 33 516 1174 1335 2020 3013 3204 33
Size: 483 658 161 685 993 191 1121
Fragments arranged by size:
1121 993 685 658 483 191 161
SfoI GGC'GCC
Cuts at: 1724 1972 1724
Size: 248 4044
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4292
SmaI CCC'GGG
Cuts at: 241 1057 1228 1314 241
Size: 816 171 86 3219
Fragments arranged by size:
3219 816 171 86
SmlI C'TyrA_G
Cuts at: 1447 2362 2854 3116 1447
Size: 915 492 262 2623
Fragments arranged by size:
2623 915 492 262
SnaBI TAC'GTA
Cuts at: 1284 1284
Size: 4292
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4292
SphI G_CATG'C
Cuts at: 1345 1845 1345
Size: 500 3792
StyI C'CwwG_G
Cuts at: 125 617 1426 1780 125
Size: 492 809 354 2637
Fragments arranged by size:
2637 809 492 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 553 754 1065 1875 2008 2467 2676 553
Size: 201 311 810 133 459 209 2169
Fragments arranged by size:
2169 810 459 311 209 201 133
TatI w'GTAC_w
Cuts at: 371 634 3807 371
Size: 263 3173 856
Fragments arranged by size:
3173 856 263
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 470 502 3809 3841 470
Size: 32 3307 32 921
Fragments arranged by size:
3307 921 32 32
Tth111I GACn'n_nGTC
Cuts at: 2089 2089
Size: 4292
XbaI T'CTAG_A
Cuts at: 1323 1323
Size: 4292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 345 1787 132
Size: 213 1442 2637
Fragments arranged by size:
2637 1442 213
XmaI C'CCGG_G
Cuts at: 239 1055 1226 1312 239
Size: 816 171 86 3219
Fragments arranged by size:
3219 816 171 86
XmnI GAAnn'nnTTC
Cuts at: 311 311
Size: 4292
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BssHII BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PshAI PsiI PspXI PsrI SacII SexAI
SfiI SgrAI SrfI SspI StuI SwaI XhoI ZraI