Restriction map for
pF1KSDA1165
(Circular) (Six-base) MAPSORT of: pF1KSDA1165.seq Check: 8748 from: 1 to: 3865
pF1KSDA1165 3865 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 901 901
Size: 3865
Acc65I G'GTAC_C
Cuts at: 879 879
Size: 3865
AclI AA'CG_TT
Cuts at: 3705 3705
Size: 3865
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 311 483 750 1688 2120 2869 3137 239
Size: 72 172 267 938 432 749 268 967
Fragments arranged by size:
967 938 749 432 268 267 172 72
AflIII A'CryG_T
Cuts at: 800 1215 2321 3298 800
Size: 415 1106 977 1367
Fragments arranged by size:
1367 1106 977 415
AgeI A'CCGG_T
Cuts at: 2253 2253
Size: 3865
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 559 559
Size: 3865
AlwNI CAG_nnn'CTG
Cuts at: 1168 2737 1168
Size: 1569 2296
ApaLI G'TGCA_C
Cuts at: 2635 2635
Size: 3865
ApoI r'AATT_y
Cuts at: 464 867 3218 3474 464
Size: 403 2351 256 855
Fragments arranged by size:
2351 855 403 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3865
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3865
AvaI C'yCGr_G
Cuts at: 883 883
Size: 3865
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 323 356 323
Size: 33 3832
BamHI G'GATC_C
Cuts at: 668 888 668
Size: 220 3645
BanI G'GyrC_C
Cuts at: 879 1293 1541 1576 879
Size: 414 248 35 3168
Fragments arranged by size:
3168 414 248 35
BanII G_rGCy'C
Cuts at: 877 1907 877
Size: 1030 2835
BbeI G_GCGC'C
Cuts at: 1297 1545 1297
Size: 248 3617
BbsI GAAGACnn'nnnn_
Cuts at: 245 245
Size: 3865
BciVI GTATCCnnnnn_n'
Cuts at: 1761 2524 3865 1761
Size: 763 1341 1761
Fragments arranged by size:
1761 1341 763
BclI T'GATC_A
Cuts at: 659 1383 659
Size: 724 3141
BglII A'GATC_T
Cuts at: 1378 3417 1378
Size: 2039 1826
BlpI GC'TnA_GC
Cuts at: 975 975
Size: 3865
Bme1580I G_kGCm'C
Cuts at: 1488 1581 2639 1488
Size: 93 1058 2714
Fragments arranged by size:
2714 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 158 1075 1156 1491 3637 158
Size: 917 81 335 2146 386
Fragments arranged by size:
2146 917 386 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 201 2230 201
Size: 2029 1836
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1039 1918 2412 2710 1039
Size: 879 494 298 2194
Fragments arranged by size:
2194 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 3569 3569
Size: 3865
BsaAI yAC'GTr
Cuts at: 855 1216 855
Size: 361 3504
BsaBI GATnn'nnATC
Cuts at: 6 1377 3416 6
Size: 1371 2039 455
Fragments arranged by size:
2039 1371 455
BsaHI Gr'CG_yC
Cuts at: 1294 1542 2246 3742 1294
Size: 248 704 1496 1417
Fragments arranged by size:
1496 1417 704 248
BsaWI w'CCGG_w
Cuts at: 1052 1573 2253 2527 2674 3085 1052
Size: 521 680 274 147 411 1832
Fragments arranged by size:
1832 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 463 493 463
Size: 30 3835
BseYI C'CCAG_C
Cuts at: 225 1288 2046 2625 3121 225
Size: 1063 758 579 496 969
Fragments arranged by size:
1063 969 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 262
Size: 3865
BsiEI CG_ry'CG
Cuts at: 104 1405 1451 2661 104
Size: 1301 46 1210 1308
Fragments arranged by size:
1308 1301 1210 46
BsiHKAI G_wGCw'C
Cuts at: 877 1655 1845 2639 877
Size: 778 190 794 2103
Fragments arranged by size:
2103 794 778 190
Bsp1286I G_dGCh'C
Cuts at: 877 1488 1581 1655 1845 1907 2639 877
Size: 611 93 74 190 62 732 2103
Fragments arranged by size:
2103 732 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3085 3085
Size: 3865
BspHI T'CATG_A
Cuts at: 3041 3041
Size: 3865
BspMI ACCTGCnnnn'nnnn_
Cuts at: 913 1127 1429 1810 913
Size: 214 302 381 2968
Fragments arranged by size:
2968 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2155 2209 3695 3860 50
Size: 2105 54 1486 165 55
Fragments arranged by size:
2105 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 437 1775 437
Size: 1338 2527
BsrFI r'CCGG_y
Cuts at: 1861 2042 2253 1861
Size: 181 211 3473
Fragments arranged by size:
3473 211 181
BssSI C'ACGA_G
Cuts at: 2134 2494 2134
Size: 360 3505
BstAPI GCAn_nnn'nTGC
Cuts at: 240 1240 240
Size: 1000 2865
BstBI TT'CG_AA
Cuts at: 110 110
Size: 3865
BstYI r'GATC_y
Cuts at: 1 668 888 1355 1378 1713 1959 2973 3059
Size: 667 220 467 23 335 246 1014 86
Cuts at: 3059 3071 3417 1
Size: 12 346 449
Fragments arranged by size:
1014 667 467 449 346 335 246 220
86 23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 3865
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1813 1994 2157 1813
Size: 181 163 3521
Fragments arranged by size:
3521 181 163
BtsI GCAGTG_nn'
Cuts at: 1106 1106
Size: 3865
DraI TTT'AAA
Cuts at: 862 862
Size: 3865
DrdI GACnn_nn'nnGTC
Cuts at: 1569 2429 1569
Size: 860 3005
EaeI y'GGCC_r
Cuts at: 1448 1622 2013 2040 3387 1448
Size: 174 391 27 1347 1926
Fragments arranged by size:
1926 1347 391 174 27
EagI C'GGCC_G
Cuts at: 1448 1448
Size: 3865
EarI CTCTTCn'nnn_
Cuts at: 170 285 395 470 1886 2096 170
Size: 115 110 75 1416 210 1939
Fragments arranged by size:
1939 1416 210 115 110 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2071 2383 2529 3675 2071
Size: 312 146 1146 2261
Fragments arranged by size:
2261 1146 312 146
EcoICRI GAG'CTC
Cuts at: 875 875
Size: 3865
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 311 483 750 1688 2120 2869 3137 239
Size: 72 172 267 938 432 749 268 967
Fragments arranged by size:
967 938 749 432 268 267 172 72
EcoO109I rG'GnC_Cy
Cuts at: 1002 1002
Size: 3865
EcoRI G'AATT_C
Cuts at: 464 867 464
Size: 403 3462
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1882 1882
Size: 3865
FspI TGC'GCA
Cuts at: 1644 1644
Size: 3865
HaeII r_GCGC'y
Cuts at: 156 1297 1545 2569 3548 156
Size: 1141 248 1024 979 473
Fragments arranged by size:
1141 1024 979 473 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1705 1735 1737 1767 1705
Size: 30 2 30 3803
Fragments arranged by size:
3803 30 30 2
HincII GTy'rAC
Cuts at: 902 902
Size: 3865
KasI G'GCGC_C
Cuts at: 1293 1541 1293
Size: 248 3617
KpnI G_GTAC'C
Cuts at: 883 883
Size: 3865
MluI A'CGCG_T
Cuts at: 3298 3298
Size: 3865
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2536 2720 2536
Size: 184 3681
MscI TGG'CCA
Cuts at: 1624 1624
Size: 3865
MslI CAynn'nnrTG
Cuts at: 541 1852 1979 541
Size: 1311 127 2427
Fragments arranged by size:
2427 1311 127
MspA1I CmG'CkG
Cuts at: 439 820 974 1288 1648 2663 2908 439
Size: 381 154 314 360 1015 245 1396
Fragments arranged by size:
1396 1015 381 360 314 245 154
NaeI GCC'GGC
Cuts at: 2044 2044
Size: 3865
NarI GG'CG_CC
Cuts at: 1294 1542 1294
Size: 248 3617
NcoI C'CATG_G
Cuts at: 125 125
Size: 3865
NgoMIV G'CCGG_C
Cuts at: 2042 2042
Size: 3865
NruI TCG'CGA
Cuts at: 2226 2226
Size: 3865
NspI r_CATG'y
Cuts at: 804 916 1416 2325 804
Size: 112 500 909 2344
Fragments arranged by size:
2344 909 500 112
PciI A'CATG_T
Cuts at: 800 2321 800
Size: 1521 2344
PmeI GTTT'AAAC
Cuts at: 862 862
Size: 3865
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3004 3036 3004
Size: 32 3833
PstI C_TGCA'G
Cuts at: 444 910 1595 444
Size: 466 685 2714
Fragments arranged by size:
2714 685 466
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3865
PvuII CAG'CTG
Cuts at: 439 820 1288 1648 439
Size: 381 468 360 2656
Fragments arranged by size:
2656 468 381 360
RsrII CG'GwC_CG
Cuts at: 2058 2058
Size: 3865
SacI G_AGCT'C
Cuts at: 877 877
Size: 3865
SalI G'TCGA_C
Cuts at: 900 900
Size: 3865
SapI GCTCTTCn'nnn_
Cuts at: 1886 2096 1886
Size: 210 3655
SbfI CC_TGCA'GG
Cuts at: 910 910
Size: 3865
ScaI AGT'ACT
Cuts at: 3382 3382
Size: 3865
SexAI A'CCwGG_T
Cuts at: 158 158
Size: 3865
SfcI C'TryA_G
Cuts at: 33 200 372 440 906 1591 2586 2777 33
Size: 167 172 68 466 685 995 191 1121
Fragments arranged by size:
1121 995 685 466 191 172 167 68
SfoI GGC'GCC
Cuts at: 1295 1543 1295
Size: 248 3617
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3865
SmaI CCC'GGG
Cuts at: 885 885
Size: 3865
SmlI C'TyrA_G
Cuts at: 1018 1933 2427 2689 1018
Size: 915 494 262 2194
Fragments arranged by size:
2194 915 494 262
SnaBI TAC'GTA
Cuts at: 855 855
Size: 3865
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3865
SphI G_CATG'C
Cuts at: 916 1416 916
Size: 500 3365
StyI C'CwwG_G
Cuts at: 125 997 1351 125
Size: 872 354 2639
Fragments arranged by size:
2639 872 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1446 1579 2038 2249 1446
Size: 133 459 211 3062
Fragments arranged by size:
3062 459 211 133
TatI w'GTAC_w
Cuts at: 744 3380 744
Size: 2636 1229
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 463 495 3382 3414 463
Size: 32 2887 32 914
Fragments arranged by size:
2887 914 32 32
Tth111I GACn'n_nGTC
Cuts at: 1660 1660
Size: 3865
XbaI T'CTAG_A
Cuts at: 894 894
Size: 3865
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 145 1358 145
Size: 1213 2652
XmaI C'CCGG_G
Cuts at: 883 883
Size: 3865
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SrfI SspI StuI SwaI XhoI XmnI ZraI