Restriction map for
pF1KSDA1158
(Circular) (Six-base) MAPSORT of: pF1KSDA1158.seq Check: 4549 from: 1 to: 3782
pF1KSDA1158 3782 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 490 490
Size: 3782
AccI GT'mk_AC
Cuts at: 820 820
Size: 3782
Acc65I G'GTAC_C
Cuts at: 756 798 756
Size: 42 3740
AclI AA'CG_TT
Cuts at: 3622 3622
Size: 3782
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1607 2039 2786 3054 1607
Size: 432 747 268 2335
Fragments arranged by size:
2335 747 432 268
AflIII A'CryG_T
Cuts at: 431 707 1134 2238 3215 431
Size: 276 427 1104 977 998
Fragments arranged by size:
1104 998 977 427 276
AgeI A'CCGG_T
Cuts at: 2170 2170
Size: 3782
AleI CACnn'nnGTG
Cuts at: 298 301 589 298
Size: 3 288 3491
Fragments arranged by size:
3491 288 3
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 439 439
Size: 3782
AlwNI CAG_nnn'CTG
Cuts at: 188 1087 2654 188
Size: 899 1567 1316
Fragments arranged by size:
1567 1316 899
ApaLI G'TGCA_C
Cuts at: 2552 2552
Size: 3782
ApoI r'AATT_y
Cuts at: 786 3135 3391 786
Size: 2349 256 1177
Fragments arranged by size:
2349 1177 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3782
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3782
AvaI C'yCGr_G
Cuts at: 322 494 802 322
Size: 172 308 3302
Fragments arranged by size:
3302 308 172
BamHI G'GATC_C
Cuts at: 807 807
Size: 3782
BanI G'GyrC_C
Cuts at: 395 756 798 1212 1460 1495 395
Size: 361 42 414 248 35 2682
Fragments arranged by size:
2682 414 361 248 42 35
BanII G_rGCy'C
Cuts at: 385 796 1826 385
Size: 411 1030 2341
Fragments arranged by size:
2341 1030 411
BbeI G_GCGC'C
Cuts at: 399 1216 1464 399
Size: 817 248 2717
Fragments arranged by size:
2717 817 248
BbsI GAAGACnn'nnnn_
Cuts at: 538 618 538
Size: 80 3702
BciVI GTATCCnnnnn_n'
Cuts at: 1680 2441 3782 1680
Size: 761 1341 1680
Fragments arranged by size:
1680 1341 761
BclI T'GATC_A
Cuts at: 1302 1302
Size: 3782
BglI GCCn_nnn'nGGC
Cuts at: 691 691
Size: 3782
BglII A'GATC_T
Cuts at: 1297 3334 1297
Size: 2037 1745
BlpI GC'TnA_GC
Cuts at: 894 894
Size: 3782
Bme1580I G_kGCm'C
Cuts at: 215 1407 1500 2556 215
Size: 1192 93 1056 1441
Fragments arranged by size:
1441 1192 1056 93
BmgBI CAC'GTC
Cuts at: 432 432
Size: 3782
BmrI ACTGGGnnnn_n'
Cuts at: 186 994 1075 1410 3554 186
Size: 808 81 335 2144 414
Fragments arranged by size:
2144 808 414 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2149 2149
Size: 3782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 593 593
Size: 3782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 958 1837 2329 2627 958
Size: 879 492 298 2113
Fragments arranged by size:
2113 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 600 681 3486 600
Size: 81 2805 896
Fragments arranged by size:
2805 896 81
BsaAI yAC'GTr
Cuts at: 774 1135 774
Size: 361 3421
BsaBI GATnn'nnATC
Cuts at: 6 613 1296 3333 6
Size: 607 683 2037 455
Fragments arranged by size:
2037 683 607 455
BsaHI Gr'CG_yC
Cuts at: 396 1213 1461 2163 3659 396
Size: 817 248 702 1496 519
Fragments arranged by size:
1496 817 702 519 248
BsaWI w'CCGG_w
Cuts at: 971 1492 2170 2444 2591 3002 971
Size: 521 678 274 147 411 1751
Fragments arranged by size:
1751 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 490 520 490
Size: 30 3752
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 295 580 295
Size: 285 3497
BseYI C'CCAG_C
Cuts at: 1207 1965 2542 3038 1207
Size: 758 577 496 1951
Fragments arranged by size:
1951 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 253
Size: 3782
BsiEI CG_ry'CG
Cuts at: 104 1324 1370 2578 104
Size: 1220 46 1208 1308
Fragments arranged by size:
1308 1220 1208 46
BsiHKAI G_wGCw'C
Cuts at: 449 796 1574 1764 2556 449
Size: 347 778 190 792 1675
Fragments arranged by size:
1675 792 778 347 190
BsmBI CGTCTCn'nnnn_
Cuts at: 214 214
Size: 3782
Bsp1286I G_dGCh'C
Cuts at: 215 385 449 796 1407 1500 1574 1764 1826
Size: 170 64 347 611 93 74 190 62
Cuts at: 1826 2556 215
Size: 730 1441
Fragments arranged by size:
1441 730 611 347 190 170 93 74
64 62
BspEI T'CCGG_A
Cuts at: 3002 3002
Size: 3782
BspHI T'CATG_A
Cuts at: 605 2958 605
Size: 2353 1429
BspMI ACCTGCnnnn'nnnn_
Cuts at: 431 490 832 1046 1348 1729 431
Size: 59 342 214 302 381 2484
Fragments arranged by size:
2484 381 342 302 214 59
BsrBI CCG'CTC
Cuts at: 50 2074 2128 3612 3777 50
Size: 2024 54 1484 165 55
Fragments arranged by size:
2024 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 492 1694 492
Size: 1202 2580
BsrFI r'CCGG_y
Cuts at: 1780 1961 2170 1780
Size: 181 209 3392
Fragments arranged by size:
3392 209 181
BsrGI T'GTAC_A
Cuts at: 611 611
Size: 3782
BssSI C'ACGA_G
Cuts at: 163 2053 2411 163
Size: 1890 358 1534
Fragments arranged by size:
1890 1534 358
BstAPI GCAn_nnn'nTGC
Cuts at: 266 1159 266
Size: 893 2889
BstBI TT'CG_AA
Cuts at: 110 2143 110
Size: 2033 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 300 303 300
Size: 3 3779
BstYI r'GATC_y
Cuts at: 1 807 1274 1297 1632 1878 2890 2976 2988
Size: 806 467 23 335 246 1012 86 12
Cuts at: 2988 3334 1
Size: 346 449
Fragments arranged by size:
1012 806 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 296 296
Size: 3782
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1732 1913 2076 1732
Size: 181 163 3438
Fragments arranged by size:
3438 181 163
BtsI GCAGTG_nn'
Cuts at: 409 1025 409
Size: 616 3166
DraI TTT'AAA
Cuts at: 781 781
Size: 3782
DraIII CAC_nnn'GTG
Cuts at: 638 638
Size: 3782
DrdI GACnn_nn'nnGTC
Cuts at: 1488 2346 1488
Size: 858 2924
EaeI y'GGCC_r
Cuts at: 363 1367 1541 1932 1959 3304 363
Size: 1004 174 391 27 1345 841
Fragments arranged by size:
1345 1004 841 391 174 27
EagI C'GGCC_G
Cuts at: 1367 1367
Size: 3782
EarI CTCTTCn'nnn_
Cuts at: 268 683 1805 2015 268
Size: 415 1122 210 2035
Fragments arranged by size:
2035 1122 415 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1990 2300 2446 3592 1990
Size: 310 146 1146 2180
Fragments arranged by size:
2180 1146 310 146
EcoICRI GAG'CTC
Cuts at: 794 794
Size: 3782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 593 1607 2039 2786 3054 593
Size: 1014 432 747 268 1321
Fragments arranged by size:
1321 1014 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 420 921 420
Size: 501 3281
EcoRI G'AATT_C
Cuts at: 786 786
Size: 3782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 358 1801 358
Size: 1443 2339
FspI TGC'GCA
Cuts at: 1563 1563
Size: 3782
HaeII r_GCGC'y
Cuts at: 399 1216 1464 2486 3465 399
Size: 817 248 1022 979 716
Fragments arranged by size:
1022 979 817 716 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 571 603 1624 1654 1656 1686 571
Size: 32 1021 30 2 30 2667
Fragments arranged by size:
2667 1021 32 30 30 2
HincII GTy'rAC
Cuts at: 821 821
Size: 3782
KasI G'GCGC_C
Cuts at: 395 1212 1460 395
Size: 817 248 2717
Fragments arranged by size:
2717 817 248
KpnI G_GTAC'C
Cuts at: 760 802 760
Size: 42 3740
MluI A'CGCG_T
Cuts at: 707 3215 707
Size: 2508 1274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2453 2637 2453
Size: 184 3598
MscI TGG'CCA
Cuts at: 365 1543 365
Size: 1178 2604
MslI CAynn'nnrTG
Cuts at: 298 301 442 589 652 1771 1898 298
Size: 3 141 147 63 1119 127 2182
Fragments arranged by size:
2182 1119 147 141 127 63 3
MspA1I CmG'CkG
Cuts at: 893 1207 1567 2580 2825 893
Size: 314 360 1013 245 1850
Fragments arranged by size:
1850 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1963 1963
Size: 3782
NarI GG'CG_CC
Cuts at: 396 1213 1461 396
Size: 817 248 2717
Fragments arranged by size:
2717 817 248
NgoMIV G'CCGG_C
Cuts at: 1961 1961
Size: 3782
NspI r_CATG'y
Cuts at: 835 1335 2242 835
Size: 500 907 2375
Fragments arranged by size:
2375 907 500
PciI A'CATG_T
Cuts at: 2238 2238
Size: 3782
PflMI CCAn_nnn'nTGG
Cuts at: 375 375
Size: 3782
PmeI GTTT'AAAC
Cuts at: 781 781
Size: 3782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2921 2953 2921
Size: 32 3750
PpuMI rG'GwC_Cy
Cuts at: 420 420
Size: 3782
PstI C_TGCA'G
Cuts at: 404 428 829 1514 404
Size: 24 401 685 2672
Fragments arranged by size:
2672 685 401 24
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3782
PvuII CAG'CTG
Cuts at: 1207 1567 1207
Size: 360 3422
RsrII CG'GwC_CG
Cuts at: 1977 1977
Size: 3782
SacI G_AGCT'C
Cuts at: 796 796
Size: 3782
SalI G'TCGA_C
Cuts at: 819 819
Size: 3782
SapI GCTCTTCn'nnn_
Cuts at: 1805 2015 1805
Size: 210 3572
SbfI CC_TGCA'GG
Cuts at: 428 829 428
Size: 401 3381
ScaI AGT'ACT
Cuts at: 3299 3299
Size: 3782
SfcI C'TryA_G
Cuts at: 33 315 400 424 666 825 1510 2503 2694
Size: 282 85 24 242 159 685 993 191
Cuts at: 2694 33
Size: 1121
Fragments arranged by size:
1121 993 685 282 242 191 159 85
24
SfoI GGC'GCC
Cuts at: 397 1214 1462 397
Size: 817 248 2717
Fragments arranged by size:
2717 817 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3782
SmaI CCC'GGG
Cuts at: 496 804 496
Size: 308 3474
SmlI C'TyrA_G
Cuts at: 937 1852 2344 2606 937
Size: 915 492 262 2113
Fragments arranged by size:
2113 915 492 262
SnaBI TAC'GTA
Cuts at: 774 774
Size: 3782
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3782
SphI G_CATG'C
Cuts at: 835 1335 835
Size: 500 3282
StyI C'CwwG_G
Cuts at: 916 1270 916
Size: 354 3428
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1365 1498 1957 2166 1365
Size: 133 459 209 2981
Fragments arranged by size:
2981 459 209 133
TatI w'GTAC_w
Cuts at: 611 3297 611
Size: 2686 1096
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3299 3331 3299
Size: 32 3750
Tth111I GACn'n_nGTC
Cuts at: 1579 1579
Size: 3782
XbaI T'CTAG_A
Cuts at: 813 813
Size: 3782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 300 1277 300
Size: 977 2805
XmaI C'CCGG_G
Cuts at: 494 802 494
Size: 308 3474
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI SspI StuI SwaI XhoI XmnI ZraI