Restriction map for
pF1KSDA1074
(Circular) (Six-base) MAPSORT of: pF1KSDA1074.seq Check: 8023 from: 1 to: 8266
pF1KSDA1074 8266 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 4383 5167 5302 4383
Size: 784 135 7347
Fragments arranged by size:
7347 784 135
Acc65I G'GTAC_C
Cuts at: 5280 5280
Size: 8266
AclI AA'CG_TT
Cuts at: 8106 8106
Size: 8266
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 869 1862 2974 4051 4118 6089 6521 7270 7538
Size: 993 1112 1077 67 1971 432 749 268
Cuts at: 7538 869
Size: 1597
Fragments arranged by size:
1971 1597 1112 1077 993 749 432 268
67
AflII C'TTAA_G
Cuts at: 858 858
Size: 8266
AflIII A'CryG_T
Cuts at: 5616 6722 7699 5616
Size: 1106 977 6183
Fragments arranged by size:
6183 1106 977
AgeI A'CCGG_T
Cuts at: 6654 6654
Size: 8266
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2615 2647 3206 3238 2615
Size: 32 559 32 7643
Fragments arranged by size:
7643 559 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3961 4146 5569 7138 3961
Size: 185 1423 1569 5089
Fragments arranged by size:
5089 1569 1423 185
ApaLI G'TGCA_C
Cuts at: 1312 7036 1312
Size: 5724 2542
ApoI r'AATT_y
Cuts at: 712 996 1407 2010 2241 2753 2811 2820 3087
Size: 284 411 603 231 512 58 9 267
Cuts at: 3087 3267 4089 4264 4406 5268 7619 7875 712
Size: 180 822 175 142 862 2351 256 1103
Fragments arranged by size:
2351 1103 862 822 603 512 411 284
267 256 231 180 175 142 58 9
AseI AT'TA_AT
Cuts at: 20 1867 3617 3872 4319 20
Size: 1847 1750 255 447 3967
Fragments arranged by size:
3967 1847 1750 447 255
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8266
AvaI C'yCGr_G
Cuts at: 2720 5284 2720
Size: 2564 5702
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3448 3481 3448
Size: 33 8233
BamHI G'GATC_C
Cuts at: 5289 5289
Size: 8266
BanI G'GyrC_C
Cuts at: 223 5280 5694 5942 5977 223
Size: 5057 414 248 35 2512
Fragments arranged by size:
5057 2512 414 248 35
BanII G_rGCy'C
Cuts at: 169 5278 6308 169
Size: 5109 1030 2127
Fragments arranged by size:
5109 2127 1030
BbeI G_GCGC'C
Cuts at: 227 5698 5946 227
Size: 5471 248 2547
Fragments arranged by size:
5471 2547 248
BbsI GAAGACnn'nnnn_
Cuts at: 857 923 2399 2990 3001 3613 3673 4763 857
Size: 66 1476 591 11 612 60 1090 4360
Fragments arranged by size:
4360 1476 1090 612 591 66 60 11
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2468 2502 2701 2735 2468
Size: 34 199 34 7999
Fragments arranged by size:
7999 199 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 794 1545 4386 6162 6925 8266 794
Size: 751 2841 1776 763 1341 794
Fragments arranged by size:
2841 1776 1341 794 763 751
BclI T'GATC_A
Cuts at: 1896 3183 4496 5784 1896
Size: 1287 1313 1288 4378
Fragments arranged by size:
4378 1313 1288 1287
BglII A'GATC_T
Cuts at: 261 4716 4906 4980 5193 5779 7818 261
Size: 4455 190 74 213 586 2039 709
Fragments arranged by size:
4455 2039 709 586 213 190 74
BlpI GC'TnA_GC
Cuts at: 957 5376 957
Size: 4419 3847
Bme1580I G_kGCm'C
Cuts at: 1316 5889 5982 7040 1316
Size: 4573 93 1058 2542
Fragments arranged by size:
4573 2542 1058 93
BmgBI CAC'GTC
Cuts at: 249 249
Size: 8266
BmrI ACTGGGnnnn_n'
Cuts at: 3033 5476 5557 5892 8038 3033
Size: 2443 81 335 2146 3261
Fragments arranged by size:
3261 2443 2146 335 81
BmtI G_CTAG'C
Cuts at: 4864 4864
Size: 8266
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1416 3397 3548 6631 1416
Size: 1981 151 3083 3051
Fragments arranged by size:
3083 3051 1981 151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2662 3118 3868 4381 2662
Size: 456 750 513 6547
Fragments arranged by size:
6547 750 513 456
Bpu10I CC'TnA_GC
Cuts at: 328 3424 328
Size: 3096 5170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4628 4736 5088 5440 6319 6813 7111 4628
Size: 108 352 352 879 494 298 5783
Fragments arranged by size:
5783 879 494 352 352 298 108
BsaI GGTCTCn'nnnn_
Cuts at: 248 7970 248
Size: 7722 544
BsaAI yAC'GTr
Cuts at: 5256 5617 5256
Size: 361 7905
BsaBI GATnn'nnATC
Cuts at: 6 542 3182 3619 5778 7817 6
Size: 536 2640 437 2159 2039 455
Fragments arranged by size:
2640 2159 2039 536 455 437
BsaHI Gr'CG_yC
Cuts at: 224 5695 5943 6647 8143 224
Size: 5471 248 704 1496 347
Fragments arranged by size:
5471 1496 704 347 248
BsaWI w'CCGG_w
Cuts at: 5453 5974 6654 6928 7075 7486 5453
Size: 521 680 274 147 411 6233
Fragments arranged by size:
6233 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2615 2645 3514 3544 3841 3871 2615
Size: 30 869 30 297 30 7010
Fragments arranged by size:
7010 869 297 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 201 3535 4075 4432 201
Size: 3334 540 357 4035
Fragments arranged by size:
4035 3334 540 357
BseYI C'CCAG_C
Cuts at: 1438 5689 6447 7026 7522 1438
Size: 4251 758 579 496 2182
Fragments arranged by size:
4251 2182 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 331
Size: 8266
BsiEI CG_ry'CG
Cuts at: 104 5806 5852 7062 104
Size: 5702 46 1210 1308
Fragments arranged by size:
5702 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1266 1316 1975 2491 5278 6056 6246 7040 1266
Size: 50 659 516 2787 778 190 794 2492
Fragments arranged by size:
2787 2492 794 778 659 516 190 50
BsmI GAATG_Cn'
Cuts at: 2736 3081 2736
Size: 345 7921
Bsp1286I G_dGCh'C
Cuts at: 169 1266 1316 1975 2491 5278 5889 5982 6056
Size: 1097 50 659 516 2787 611 93 74
Cuts at: 6056 6246 6308 7040 169
Size: 190 62 732 1395
Fragments arranged by size:
2787 1395 1097 732 659 611 516 190
93 74 62 50
BspEI T'CCGG_A
Cuts at: 7486 7486
Size: 8266
BspHI T'CATG_A
Cuts at: 2832 7442 2832
Size: 4610 3656
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4360 5314 5528 5830 6211 4360
Size: 954 214 302 381 6415
Fragments arranged by size:
6415 954 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6556 6610 8096 8261 50
Size: 6506 54 1486 165 55
Fragments arranged by size:
6506 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1223 3086 6176 1223
Size: 1863 3090 3313
Fragments arranged by size:
3313 3090 1863
BsrFI r'CCGG_y
Cuts at: 6262 6443 6654 6262
Size: 181 211 7874
Fragments arranged by size:
7874 211 181
BsrGI T'GTAC_A
Cuts at: 486 1671 5223 486
Size: 1185 3552 3529
Fragments arranged by size:
3552 3529 1185
BssSI C'ACGA_G
Cuts at: 3369 6535 6895 3369
Size: 3166 360 4740
Fragments arranged by size:
4740 3166 360
BstAPI GCAn_nnn'nTGC
Cuts at: 5641 5641
Size: 8266
BstBI TT'CG_AA
Cuts at: 110 110
Size: 8266
BstXI CCAn_nnnn'nTGG
Cuts at: 3046 3046
Size: 8266
BstYI r'GATC_y
Cuts at: 1 261 272 317 4716 4906 4980 5193 5289
Size: 260 11 45 4399 190 74 213 96
Cuts at: 5289 5756 5779 6114 6360 7374 7460 7472 7818
Size: 467 23 335 246 1014 86 12 346
Cuts at: 7818 1
Size: 449
Fragments arranged by size:
4399 1014 467 449 346 335 260 246
213 190 96 86 74 45 23 12
11
BtgI C'CryG_G
Cuts at: 558 4275 5035 558
Size: 3717 760 3789
Fragments arranged by size:
3789 3717 760
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6214 6395 6558 6214
Size: 181 163 7922
Fragments arranged by size:
7922 181 163
BtsI GCAGTG_nn'
Cuts at: 566 5507 566
Size: 4941 3325
DraI TTT'AAA
Cuts at: 3324 3899 4286 5234 5263 3324
Size: 575 387 948 29 6327
Fragments arranged by size:
6327 948 575 387 29
DraIII CAC_nnn'GTG
Cuts at: 4954 4954
Size: 8266
DrdI GACnn_nn'nnGTC
Cuts at: 5970 6830 5970
Size: 860 7406
EaeI y'GGCC_r
Cuts at: 5849 6023 6414 6441 7788 5849
Size: 174 391 27 1347 6327
Fragments arranged by size:
6327 1347 391 174 27
EagI C'GGCC_G
Cuts at: 5849 5849
Size: 8266
EarI CTCTTCn'nnn_
Cuts at: 494 578 1715 2513 2518 2576 6287 6497 494
Size: 84 1137 798 5 58 3711 210 2263
Fragments arranged by size:
3711 2263 1137 798 210 84 58 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 198 6472 6784 6930 8076 198
Size: 6274 312 146 1146 388
Fragments arranged by size:
6274 1146 388 312 146
EcoICRI GAG'CTC
Cuts at: 5276 5276
Size: 8266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 869 1862 2662 2974 3118 3868 4051 4118 4381
Size: 993 800 312 144 750 183 67 263
Cuts at: 4381 6089 6521 7270 7538 869
Size: 1708 432 749 268 1597
Fragments arranged by size:
1708 1597 993 800 750 749 432 312
268 263 183 144 67
EcoNI CCTnn'n_nnAGG
Cuts at: 861 861
Size: 8266
EcoO109I rG'GnC_Cy
Cuts at: 2955 3420 5403 2955
Size: 465 1983 5818
Fragments arranged by size:
5818 1983 465
EcoRI G'AATT_C
Cuts at: 2010 2753 2811 2820 5268 2010
Size: 743 58 9 2448 5008
Fragments arranged by size:
5008 2448 743 58 9
EcoRV GAT'ATC
Cuts at: 1603 1603
Size: 8266
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 357 4236 6283 357
Size: 3879 2047 2340
Fragments arranged by size:
3879 2340 2047
FspI TGC'GCA
Cuts at: 6045 6045
Size: 8266
HaeII r_GCGC'y
Cuts at: 227 5698 5946 6970 7949 227
Size: 5471 248 1024 979 544
Fragments arranged by size:
5471 1024 979 544 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 586 618 829 861 963 995 1324 1356 1360
Size: 32 211 32 102 32 329 32 4
Cuts at: 1360 1392 3294 3326 3514 3546 3841 3873 3972
Size: 32 1902 32 188 32 295 32 99
Cuts at: 3972 4004 4329 4361 4411 4443 4708 4740 6106
Size: 32 325 32 50 32 265 32 1366
Cuts at: 6106 6136 6138 6168 586
Size: 30 2 30 2684
Fragments arranged by size:
2684 1902 1366 329 325 295 265 211
188 102 99 50 32 32 32 32
32 32 32 32 32 32 32 32
30 30 4 2
HincII GTy'rAC
Cuts at: 4872 5303 4872
Size: 431 7835
HindIII A'AGCT_T
Cuts at: 954 4000 954
Size: 3046 5220
KasI G'GCGC_C
Cuts at: 223 5694 5942 223
Size: 5471 248 2547
Fragments arranged by size:
5471 2547 248
KpnI G_GTAC'C
Cuts at: 5284 5284
Size: 8266
MfeI C'AATT_G
Cuts at: 3928 3928
Size: 8266
MluI A'CGCG_T
Cuts at: 7699 7699
Size: 8266
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 737 4463 5069 6937 7121 737
Size: 3726 606 1868 184 1882
Fragments arranged by size:
3726 1882 1868 606 184
MscI TGG'CCA
Cuts at: 6025 6025
Size: 8266
MslI CAynn'nnrTG
Cuts at: 1627 1823 2831 3179 4149 6253 6380 1627
Size: 196 1008 348 970 2104 127 3513
Fragments arranged by size:
3513 2104 1008 970 348 196 127
MspA1I CmG'CkG
Cuts at: 1690 3070 5375 5689 6049 7064 7309 1690
Size: 1380 2305 314 360 1015 245 2647
Fragments arranged by size:
2647 2305 1380 1015 360 314 245
NaeI GCC'GGC
Cuts at: 6445 6445
Size: 8266
NarI GG'CG_CC
Cuts at: 224 5695 5943 224
Size: 5471 248 2547
Fragments arranged by size:
5471 2547 248
NcoI C'CATG_G
Cuts at: 558 4275 558
Size: 3717 4549
NdeI CA'TA_TG
Cuts at: 1011 1011
Size: 8266
NgoMIV G'CCGG_C
Cuts at: 6443 6443
Size: 8266
NheI G'CTAG_C
Cuts at: 4860 4860
Size: 8266
NruI TCG'CGA
Cuts at: 6627 6627
Size: 8266
NspI r_CATG'y
Cuts at: 1572 2871 5317 5817 6726 1572
Size: 1299 2446 500 909 3112
Fragments arranged by size:
3112 2446 1299 909 500
PciI A'CATG_T
Cuts at: 6722 6722
Size: 8266
PflMI CCAn_nnn'nTGG
Cuts at: 2023 2640 2023
Size: 617 7649
PfoI T'CCnGG_A
Cuts at: 988 2306 988
Size: 1318 6948
PmeI GTTT'AAAC
Cuts at: 5263 5263
Size: 8266
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2615 2647 7405 7437 2615
Size: 32 4758 32 3444
Fragments arranged by size:
4758 3444 32 32
PpuMI rG'GwC_Cy
Cuts at: 2955 3420 2955
Size: 465 7801
PstI C_TGCA'G
Cuts at: 1449 4371 5311 5996 1449
Size: 2922 940 685 3719
Fragments arranged by size:
3719 2922 940 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8266
PvuII CAG'CTG
Cuts at: 1690 3070 5689 6049 1690
Size: 1380 2619 360 3907
Fragments arranged by size:
3907 2619 1380 360
RsrII CG'GwC_CG
Cuts at: 6459 6459
Size: 8266
SacI G_AGCT'C
Cuts at: 5278 5278
Size: 8266
SalI G'TCGA_C
Cuts at: 5301 5301
Size: 8266
SapI GCTCTTCn'nnn_
Cuts at: 578 1715 2576 6287 6497 578
Size: 1137 861 3711 210 2347
Fragments arranged by size:
3711 2347 1137 861 210
SbfI CC_TGCA'GG
Cuts at: 5311 5311
Size: 8266
ScaI AGT'ACT
Cuts at: 7783 7783
Size: 8266
SfcI C'TryA_G
Cuts at: 33 1445 3515 4367 4462 5307 5992 6987 7178
Size: 1412 2070 852 95 845 685 995 191
Cuts at: 7178 33
Size: 1121
Fragments arranged by size:
2070 1412 1121 995 852 845 685 191
95
SfoI GGC'GCC
Cuts at: 225 5696 5944 225
Size: 5471 248 2547
Fragments arranged by size:
5471 2547 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8266
SmaI CCC'GGG
Cuts at: 5286 5286
Size: 8266
SmlI C'TyrA_G
Cuts at: 858 2720 4643 4751 5067 5419 6334 6828 7090
Size: 1862 1923 108 316 352 915 494 262
Cuts at: 7090 858
Size: 2034
Fragments arranged by size:
2034 1923 1862 915 494 352 316 262
108
SnaBI TAC'GTA
Cuts at: 5256 5256
Size: 8266
SpeI A'CTAG_T
Cuts at: 67 4291 67
Size: 4224 4042
SphI G_CATG'C
Cuts at: 1572 5317 5817 1572
Size: 3745 500 4021
Fragments arranged by size:
4021 3745 500
SspI AAT'ATT
Cuts at: 639 639
Size: 8266
StuI AGG'CCT
Cuts at: 2109 2109
Size: 8266
StyI C'CwwG_G
Cuts at: 161 558 1381 4275 5398 5752 161
Size: 397 823 2894 1123 354 2675
Fragments arranged by size:
2894 2675 1123 823 397 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 272 5847 5980 6439 6650 272
Size: 5575 133 459 211 1888
Fragments arranged by size:
5575 1888 459 211 133
TatI w'GTAC_w
Cuts at: 486 1671 2406 4440 5223 7781 486
Size: 1185 735 2034 783 2558 971
Fragments arranged by size:
2558 2034 1185 971 783 735
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7783 7815 7783
Size: 32 8234
Tth111I GACn'n_nGTC
Cuts at: 6061 6061
Size: 8266
XbaI T'CTAG_A
Cuts at: 3154 4800 5295 3154
Size: 1646 495 6125
Fragments arranged by size:
6125 1646 495
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4884 4938 5759 4884
Size: 54 821 7391
Fragments arranged by size:
7391 821 54
XhoI C'TCGA_G
Cuts at: 2720 2720
Size: 8266
XmaI C'CCGG_G
Cuts at: 5284 5284
Size: 8266
XmnI GAAnn'nnTTC
Cuts at: 1091 2987 1091
Size: 1896 6370
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NruI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI ApaI AscI
AvrII BbvCI BfrBI BglI BsiWI BsmBI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI FseI FspAI HpaI NotI
NsiI PacI PasI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI SwaI
ZraI