Restriction map for
pF1KSDA1073
(Circular) (Six-base) MAPSORT of: pF1KSDA1073.seq Check: 7983 from: 1 to: 5068
pF1KSDA1073 5068 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AatII G_ACGT'C
Cuts at: 1360 1360
Size: 5068
AccI GT'mk_AC
Cuts at: 2054 2104 2054
Size: 50 5018
Acc65I G'GTAC_C
Cuts at: 2082 2082
Size: 5068
AclI AA'CG_TT
Cuts at: 4908 4908
Size: 5068
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 891 1930 2891 3323 4072 4340 891
Size: 1039 961 432 749 268 1619
Fragments arranged by size:
1619 1039 961 749 432 268
AflII C'TTAA_G
Cuts at: 1222 1222
Size: 5068
AflIII A'CryG_T
Cuts at: 2418 3524 4501 2418
Size: 1106 977 2985
Fragments arranged by size:
2985 1106 977
AgeI A'CCGG_T
Cuts at: 3456 3456
Size: 5068
AhdI GACnn_n'nnGTC
Cuts at: 198 198
Size: 5068
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1157 1189 1157
Size: 32 5036
AlwNI CAG_nnn'CTG
Cuts at: 253 2371 3940 253
Size: 2118 1569 1381
Fragments arranged by size:
2118 1569 1381
ApaLI G'TGCA_C
Cuts at: 3838 3838
Size: 5068
ApoI r'AATT_y
Cuts at: 228 564 603 782 1621 2070 4421 4677 228
Size: 336 39 179 839 449 2351 256 619
Fragments arranged by size:
2351 839 619 449 336 256 179 39
AseI AT'TA_AT
Cuts at: 20 20
Size: 5068
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5068
AvaI C'yCGr_G
Cuts at: 138 2086 138
Size: 1948 3120
BamHI G'GATC_C
Cuts at: 483 2091 483
Size: 1608 3460
BanI G'GyrC_C
Cuts at: 2082 2496 2744 2779 2082
Size: 414 248 35 4371
Fragments arranged by size:
4371 414 248 35
BanII G_rGCy'C
Cuts at: 139 1323 1850 2080 3110 139
Size: 1184 527 230 1030 2097
Fragments arranged by size:
2097 1184 1030 527 230
BbeI G_GCGC'C
Cuts at: 2500 2748 2500
Size: 248 4820
BbsI GAAGACnn'nnnn_
Cuts at: 1360 1778 1360
Size: 418 4650
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1535 1569 1535
Size: 34 5034
BciVI GTATCCnnnnn_n'
Cuts at: 934 1429 2964 3727 5068 934
Size: 495 1535 763 1341 934
Fragments arranged by size:
1535 1341 934 763 495
BclI T'GATC_A
Cuts at: 2586 2586
Size: 5068
BfrBI ATG'CAT
Cuts at: 673 673
Size: 5068
BglI GCCn_nnn'nGGC
Cuts at: 1117 1117
Size: 5068
BglII A'GATC_T
Cuts at: 2581 4620 2581
Size: 2039 3029
BlpI GC'TnA_GC
Cuts at: 1400 2178 1400
Size: 778 4290
Bme1580I G_kGCm'C
Cuts at: 2691 2784 3842 2691
Size: 93 1058 3917
Fragments arranged by size:
3917 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 922 1053 2278 2359 2694 4840 922
Size: 131 1225 81 335 2146 1150
Fragments arranged by size:
2146 1225 1150 335 131 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 153 3433 153
Size: 3280 1788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 819 819
Size: 5068
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2242 3121 3615 3913 2242
Size: 879 494 298 3397
Fragments arranged by size:
3397 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4772 4772
Size: 5068
BsaAI yAC'GTr
Cuts at: 2058 2419 2058
Size: 361 4707
BsaBI GATnn'nnATC
Cuts at: 6 1151 2580 4619 6
Size: 1145 1429 2039 455
Fragments arranged by size:
2039 1429 1145 455
BsaHI Gr'CG_yC
Cuts at: 1357 2497 2745 3449 4945 1357
Size: 1140 248 704 1496 1480
Fragments arranged by size:
1496 1480 1140 704 248
BsaWI w'CCGG_w
Cuts at: 2255 2776 3456 3730 3877 4288 2255
Size: 521 680 274 147 411 3035
Fragments arranged by size:
3035 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1219 1249 1219
Size: 30 5038
BseYI C'CCAG_C
Cuts at: 161 184 2491 3249 3828 4324 161
Size: 23 2307 758 579 496 905
Fragments arranged by size:
2307 905 758 579 496 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2006 2006
Size: 5068
BsiEI CG_ry'CG
Cuts at: 104 181 2608 2654 3864 104
Size: 77 2427 46 1210 1308
Fragments arranged by size:
2427 1308 1210 77 46
BsiHKAI G_wGCw'C
Cuts at: 139 1850 2080 2858 3048 3842 139
Size: 1711 230 778 190 794 1365
Fragments arranged by size:
1711 1365 794 778 230 190
BsmBI CGTCTCn'nnnn_
Cuts at: 124 124
Size: 5068
Bsp1286I G_dGCh'C
Cuts at: 139 1323 1850 2080 2691 2784 2858 3048 3110
Size: 1184 527 230 611 93 74 190 62
Cuts at: 3110 3842 139
Size: 732 1365
Fragments arranged by size:
1365 1184 732 611 527 230 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4288 4288
Size: 5068
BspHI T'CATG_A
Cuts at: 4244 4244
Size: 5068
BspMI ACCTGCnnnn'nnnn_
Cuts at: 701 2116 2330 2632 3013 701
Size: 1415 214 302 381 2756
Fragments arranged by size:
2756 1415 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3358 3412 4898 5063 50
Size: 3308 54 1486 165 55
Fragments arranged by size:
3308 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1371 2978 1371
Size: 1607 3461
BsrFI r'CCGG_y
Cuts at: 165 3064 3245 3456 165
Size: 2899 181 211 1777
Fragments arranged by size:
2899 1777 211 181
BssSI C'ACGA_G
Cuts at: 3337 3697 3337
Size: 360 4708
BstAPI GCAn_nnn'nTGC
Cuts at: 2443 2443
Size: 5068
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5068
BstYI r'GATC_y
Cuts at: 1 483 643 2091 2558 2581 2916 3162 4176
Size: 482 160 1448 467 23 335 246 1014
Cuts at: 4176 4262 4274 4620 1
Size: 86 12 346 449
Fragments arranged by size:
1448 1014 482 467 449 346 335 246
160 86 23 12
BstZ17I GTA'TAC
Cuts at: 2055 2055
Size: 5068
Bsu36I CC'TnA_GG
Cuts at: 316 626 316
Size: 310 4758
BtgI C'CryG_G
Cuts at: 125 986 1521 1879 125
Size: 861 535 358 3314
Fragments arranged by size:
3314 861 535 358
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3016 3197 3360 3016
Size: 181 163 4724
Fragments arranged by size:
4724 181 163
BtsI GCAGTG_nn'
Cuts at: 1383 2309 1383
Size: 926 4142
ClaI AT'CG_AT
Cuts at: 1500 1500
Size: 5068
DraI TTT'AAA
Cuts at: 2065 2065
Size: 5068
DraIII CAC_nnn'GTG
Cuts at: 974 2029 974
Size: 1055 4013
DrdI GACnn_nn'nnGTC
Cuts at: 2772 3632 2772
Size: 860 4208
EaeI y'GGCC_r
Cuts at: 178 386 1090 1518 2651 2825 3216 3243 4590
Size: 208 704 428 1133 174 391 27 1347
Cuts at: 4590 178
Size: 656
Fragments arranged by size:
1347 1133 704 656 428 391 208 174
27
EagI C'GGCC_G
Cuts at: 178 2651 178
Size: 2473 2595
EarI CTCTTCn'nnn_
Cuts at: 3089 3299 3089
Size: 210 4858
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 648 3274 3586 3732 4878 648
Size: 2626 312 146 1146 838
Fragments arranged by size:
2626 1146 838 312 146
EcoICRI GAG'CTC
Cuts at: 137 1848 2078 137
Size: 1711 230 3127
Fragments arranged by size:
3127 1711 230
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 819 891 1930 2891 3323 4072 4340 819
Size: 72 1039 961 432 749 268 1547
Fragments arranged by size:
1547 1039 961 749 432 268 72
EcoO109I rG'GnC_Cy
Cuts at: 312 2205 312
Size: 1893 3175
EcoRI G'AATT_C
Cuts at: 228 782 1621 2070 228
Size: 554 839 449 3226
Fragments arranged by size:
3226 839 554 449
EcoRV GAT'ATC
Cuts at: 1182 1182
Size: 5068
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1738 1942 3085 1738
Size: 204 1143 3721
Fragments arranged by size:
3721 1143 204
FspI TGC'GCA
Cuts at: 2847 2847
Size: 5068
HaeII r_GCGC'y
Cuts at: 425 2500 2748 3772 4751 425
Size: 2075 248 1024 979 742
Fragments arranged by size:
2075 1024 979 742 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 184 216 908 940 1448 1480 2908 2938 2940
Size: 32 692 32 508 32 1428 30 2
Cuts at: 2940 2970 184
Size: 30 2282
Fragments arranged by size:
2282 1428 692 508 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2105 2105
Size: 5068
HindIII A'AGCT_T
Cuts at: 1303 1303
Size: 5068
KasI G'GCGC_C
Cuts at: 2496 2744 2496
Size: 248 4820
KpnI G_GTAC'C
Cuts at: 2086 2086
Size: 5068
MluI A'CGCG_T
Cuts at: 4501 4501
Size: 5068
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 972 1409 3739 3923 972
Size: 437 2330 184 2117
Fragments arranged by size:
2330 2117 437 184
MscI TGG'CCA
Cuts at: 388 1520 2827 388
Size: 1132 1307 2629
Fragments arranged by size:
2629 1307 1132
MslI CAynn'nnrTG
Cuts at: 1376 1423 1541 3055 3182 1376
Size: 47 118 1514 127 3262
Fragments arranged by size:
3262 1514 127 118 47
MspA1I CmG'CkG
Cuts at: 1667 1767 2177 2491 2851 3866 4111 1667
Size: 100 410 314 360 1015 245 2624
Fragments arranged by size:
2624 1015 410 360 314 245 100
NaeI GCC'GGC
Cuts at: 167 3247 167
Size: 3080 1988
NarI GG'CG_CC
Cuts at: 2497 2745 2497
Size: 248 4820
NcoI C'CATG_G
Cuts at: 125 986 1521 125
Size: 861 535 3672
Fragments arranged by size:
3672 861 535
NgoMIV G'CCGG_C
Cuts at: 165 3245 165
Size: 3080 1988
NruI TCG'CGA
Cuts at: 3429 3429
Size: 5068
NsiI A_TGCA'T
Cuts at: 675 675
Size: 5068
NspI r_CATG'y
Cuts at: 1836 2119 2619 3528 1836
Size: 283 500 909 3376
Fragments arranged by size:
3376 909 500 283
PciI A'CATG_T
Cuts at: 3524 3524
Size: 5068
PflMI CCAn_nnn'nTGG
Cuts at: 418 987 418
Size: 569 4499
PfoI T'CCnGG_A
Cuts at: 363 1175 363
Size: 812 4256
PmeI GTTT'AAAC
Cuts at: 2065 2065
Size: 5068
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4207 4239 4207
Size: 32 5036
PpuMI rG'GwC_Cy
Cuts at: 312 312
Size: 5068
PspXI vC'TCGA_Gb
Cuts at: 138 138
Size: 5068
PstI C_TGCA'G
Cuts at: 2113 2798 2113
Size: 685 4383
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5068
PvuII CAG'CTG
Cuts at: 1667 1767 2491 2851 1667
Size: 100 724 360 3884
Fragments arranged by size:
3884 724 360 100
RsrII CG'GwC_CG
Cuts at: 3261 3261
Size: 5068
SacI G_AGCT'C
Cuts at: 139 1850 2080 139
Size: 1711 230 3127
Fragments arranged by size:
3127 1711 230
SalI G'TCGA_C
Cuts at: 2103 2103
Size: 5068
SapI GCTCTTCn'nnn_
Cuts at: 3089 3299 3089
Size: 210 4858
SbfI CC_TGCA'GG
Cuts at: 2113 2113
Size: 5068
ScaI AGT'ACT
Cuts at: 4585 4585
Size: 5068
SfcI C'TryA_G
Cuts at: 33 1966 2109 2794 3789 3980 33
Size: 1933 143 685 995 191 1121
Fragments arranged by size:
1933 1121 995 685 191 143
SfoI GGC'GCC
Cuts at: 2498 2746 2498
Size: 248 4820
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5068
SmaI CCC'GGG
Cuts at: 2088 2088
Size: 5068
SmlI C'TyrA_G
Cuts at: 138 1222 2221 3136 3630 3892 138
Size: 1084 999 915 494 262 1314
Fragments arranged by size:
1314 1084 999 915 494 262
SnaBI TAC'GTA
Cuts at: 2058 2058
Size: 5068
SpeI A'CTAG_T
Cuts at: 67 1167 67
Size: 1100 3968
SphI G_CATG'C
Cuts at: 1836 2119 2619 1836
Size: 283 500 4285
Fragments arranged by size:
4285 500 283
SspI AAT'ATT
Cuts at: 375 873 1191 375
Size: 498 318 4252
Fragments arranged by size:
4252 498 318
StyI C'CwwG_G
Cuts at: 125 510 986 1521 2200 2554 125
Size: 385 476 535 679 354 2639
Fragments arranged by size:
2639 679 535 476 385 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 728 1842 2649 2782 3241 3452 728
Size: 1114 807 133 459 211 2344
Fragments arranged by size:
2344 1114 807 459 211 133
TatI w'GTAC_w
Cuts at: 4583 4583
Size: 5068
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4585 4617 4585
Size: 32 5036
Tth111I GACn'n_nGTC
Cuts at: 1582 1737 2863 1582
Size: 155 1126 3787
Fragments arranged by size:
3787 1126 155
XbaI T'CTAG_A
Cuts at: 2097 2097
Size: 5068
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 794 935 988 1425 2561 794
Size: 141 53 437 1136 3301
Fragments arranged by size:
3301 1136 437 141 53
XhoI C'TCGA_G
Cuts at: 138 138
Size: 5068
XmaI C'CCGG_G
Cuts at: 2086 2086
Size: 5068
XmnI GAAnn'nnTTC
Cuts at: 1901 1901
Size: 5068
ZraI GAC'GTC
Cuts at: 1358 1358
Size: 5068
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NsiI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AleI AlfI ApaI AscI AvrII BaeI
BbvCI BmgBI BmtI Bpu10I BseRI BsiWI BsmI BsrGI
BssHII BstEII BstXI CspCI EcoNI FseI FspAI HpaI
MfeI NdeI NheI NotI PacI PasI PmlI PshAI
PsiI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI StuI SwaI