Restriction map for
pF1KSDA1066
(Circular) (Six-base) MAPSORT of: pF1KSDA1066.seq Check: 2744 from: 1 to: 7147
pF1KSDA1066 7147 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4104 4104
Size: 7147
AccI GT'mk_AC
Cuts at: 1285 1469 1834 4183 1285
Size: 184 365 2349 4249
Fragments arranged by size:
4249 2349 365 184
Acc65I G'GTAC_C
Cuts at: 4161 4161
Size: 7147
AclI AA'CG_TT
Cuts at: 6987 6987
Size: 7147
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 1836 1848 1932 3139 4970 5402 6151 6419
Size: 1277 12 84 1207 1831 432 749 268
Cuts at: 6419 559
Size: 1287
Fragments arranged by size:
1831 1287 1277 1207 749 432 268 84
12
AflIII A'CryG_T
Cuts at: 752 1181 2381 3740 4497 5603 6580 752
Size: 429 1200 1359 757 1106 977 1319
Fragments arranged by size:
1359 1319 1200 1106 977 757 429
AgeI A'CCGG_T
Cuts at: 5535 5535
Size: 7147
AhdI GACnn_n'nnGTC
Cuts at: 1137 1476 2028 1137
Size: 339 552 6256
Fragments arranged by size:
6256 552 339
AleI CACnn'nnGTG
Cuts at: 830 1699 2512 2668 2731 2824 3203 830
Size: 869 813 156 63 93 379 4774
Fragments arranged by size:
4774 869 813 379 156 93 63
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 820 3851 820
Size: 3031 4116
AlwNI CAG_nnn'CTG
Cuts at: 1008 1297 1416 2836 2959 3646 3672 3960 4450
Size: 289 119 1420 123 687 26 288 490
Cuts at: 4450 6019 1008
Size: 1569 2136
Fragments arranged by size:
2136 1569 1420 687 490 289 288 123
119 26
ApaI G_GGCC'C
Cuts at: 2843 3919 2843
Size: 1076 6071
ApaLI G'TGCA_C
Cuts at: 782 3070 3325 5917 782
Size: 2288 255 2592 2012
Fragments arranged by size:
2592 2288 2012 255
ApoI r'AATT_y
Cuts at: 445 2005 4149 6500 6756 445
Size: 1560 2144 2351 256 836
Fragments arranged by size:
2351 2144 1560 836 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7147
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7147
AvaI C'yCGr_G
Cuts at: 1049 1799 2389 2593 2634 2717 2872 3724 4129
Size: 750 590 204 41 83 155 852 405
Cuts at: 4129 4165 1049
Size: 36 4031
Fragments arranged by size:
4031 852 750 590 405 204 155 83
41 36
AvrII C'CTAG_G
Cuts at: 1335 1335
Size: 7147
BamHI G'GATC_C
Cuts at: 2447 4170 2447
Size: 1723 5424
BanI G'GyrC_C
Cuts at: 824 934 2196 2826 3604 3715 3827 3858 4161
Size: 110 1262 630 778 111 112 31 303
Cuts at: 4161 4575 4823 4858 824
Size: 414 248 35 3113
Fragments arranged by size:
3113 1262 778 630 414 303 248 112
111 110 35 31
BanII G_rGCy'C
Cuts at: 229 290 1030 2369 2414 2843 2993 3051 3512
Size: 61 740 1339 45 429 150 58 461
Cuts at: 3512 3680 3919 4159 5189 229
Size: 168 239 240 1030 2187
Fragments arranged by size:
2187 1339 1030 740 461 429 240 239
168 150 61 58 45
BbeI G_GCGC'C
Cuts at: 3719 4579 4827 3719
Size: 860 248 6039
Fragments arranged by size:
6039 860 248
BbsI GAAGACnn'nnnn_
Cuts at: 2886 2886
Size: 7147
BbvCI CC'TCA_GC
Cuts at: 1498 1498
Size: 7147
BciVI GTATCCnnnnn_n'
Cuts at: 5043 5806 7147 5043
Size: 763 1341 5043
Fragments arranged by size:
5043 1341 763
BclI T'GATC_A
Cuts at: 1787 2483 4665 1787
Size: 696 2182 4269
Fragments arranged by size:
4269 2182 696
BglI GCCn_nnn'nGGC
Cuts at: 224 2815 224
Size: 2591 4556
BglII A'GATC_T
Cuts at: 4660 6699 4660
Size: 2039 5108
BlpI GC'TnA_GC
Cuts at: 334 1520 3133 3979 4257 334
Size: 1186 1613 846 278 3224
Fragments arranged by size:
3224 1613 1186 846 278
Bme1580I G_kGCm'C
Cuts at: 786 968 1050 1978 2509 2843 3074 3329 3607
Size: 182 82 928 531 334 231 255 278
Cuts at: 3607 3830 3919 4770 4863 5921 786
Size: 223 89 851 93 1058 2012
Fragments arranged by size:
2012 1058 928 851 531 334 278 255
231 223 182 93 89 82
BmgBI CAC'GTC
Cuts at: 2030 2384 2619 2892 3330 3498 2030
Size: 354 235 273 438 168 5679
Fragments arranged by size:
5679 438 354 273 235 168
BmrI ACTGGGnnnn_n'
Cuts at: 396 2725 2787 4357 4438 4773 6919 396
Size: 2329 62 1570 81 335 2146 624
Fragments arranged by size:
2329 2146 1570 624 335 81 62
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3685 5512 3685
Size: 1827 5320
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 385 553 843 1860 2142 2418 2541 334
Size: 51 168 290 1017 282 276 123 4940
Fragments arranged by size:
4940 1017 290 282 276 168 123 51
Bpu10I CC'TnA_GC
Cuts at: 1498 2269 1498
Size: 771 6376
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4321 5200 5694 5992 4321
Size: 879 494 298 5476
Fragments arranged by size:
5476 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1186 6851 1186
Size: 5665 1482
BsaAI yAC'GTr
Cuts at: 651 781 2085 2544 3741 4137 4498 651
Size: 130 1304 459 1197 396 361 3300
Fragments arranged by size:
3300 1304 1197 459 396 361 130
BsaBI GATnn'nnATC
Cuts at: 6 133 4659 6698 6
Size: 127 4526 2039 455
Fragments arranged by size:
4526 2039 455 127
BsaHI Gr'CG_yC
Cuts at: 2261 2426 2492 3371 3716 4576 4824 5528 7024
Size: 165 66 879 345 860 248 704 1496
Cuts at: 7024 2261
Size: 2384
Fragments arranged by size:
2384 1496 879 860 704 345 248 165
66
BsaWI w'CCGG_w
Cuts at: 4334 4855 5535 5809 5956 6367 4334
Size: 521 680 274 147 411 5114
Fragments arranged by size:
5114 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 169 196 199 226 571 601 1249 1279 2416
Size: 27 3 27 345 30 648 30 1137
Cuts at: 2416 2446 2698 2728 169
Size: 30 252 30 4588
Fragments arranged by size:
4588 1137 648 345 252 30 30 30
30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 286 454 592 2703 2818 4054 286
Size: 168 138 2111 115 1236 3379
Fragments arranged by size:
3379 2111 1236 168 138 115
BseYI C'CCAG_C
Cuts at: 922 1679 2299 2842 2904 2930 2992 3042 3543
Size: 757 620 543 62 26 62 50 501
Cuts at: 3543 3809 3906 4570 5328 5907 6403 922
Size: 266 97 664 758 579 496 1666
Fragments arranged by size:
1666 758 757 664 620 579 543 501
496 266 97 62 62 50 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 2010 3011 3088 3640 601
Size: 1409 1001 77 552 4108
Fragments arranged by size:
4108 1409 1001 552 77
BsiEI CG_ry'CG
Cuts at: 104 914 974 2103 2349 3297 4687 4733 5943
Size: 810 60 1129 246 948 1390 46 1210
Cuts at: 5943 104
Size: 1308
Fragments arranged by size:
1390 1308 1210 1129 948 810 246 60
46
BsiHKAI G_wGCw'C
Cuts at: 290 335 353 509 659 786 1721 1808 2084
Size: 45 18 156 150 127 935 87 276
Cuts at: 2084 2414 2891 3074 3329 4159 4937 5127 5921
Size: 330 477 183 255 830 778 190 794
Cuts at: 5921 290
Size: 1516
Fragments arranged by size:
1516 935 830 794 778 477 330 276
255 190 183 156 150 127 87 45
18
BsiWI C'GTAC_G
Cuts at: 3558 3558
Size: 7147
BsmBI CGTCTCn'nnnn_
Cuts at: 2351 2390 4010 4027 2351
Size: 39 1620 17 5471
Fragments arranged by size:
5471 1620 39 17
Bsp1286I G_dGCh'C
Cuts at: 229 290 335 353 509 659 786 968 1030
Size: 61 45 18 156 150 127 182 62
Cuts at: 1030 1050 1721 1808 1978 2084 2369 2414 2509
Size: 20 671 87 170 106 285 45 95
Cuts at: 2509 2843 2891 2993 3051 3074 3329 3512 3607
Size: 334 48 102 58 23 255 183 95
Cuts at: 3607 3680 3830 3919 4159 4770 4863 4937 5127
Size: 73 150 89 240 611 93 74 190
Cuts at: 5127 5189 5921 229
Size: 62 732 1455
Fragments arranged by size:
1455 732 671 611 334 285 255 240
190 183 182 170 156 150 150 127
106 102 95 95 93 89 87 74
73 62 62 61 58 48 45 45
23 20 18
BspEI T'CCGG_A
Cuts at: 6367 6367
Size: 7147
BspHI T'CATG_A
Cuts at: 6323 6323
Size: 7147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 672 2350 4104 4195 4409 4711 5092 672
Size: 1678 1754 91 214 302 381 2727
Fragments arranged by size:
2727 1754 1678 381 302 214 91
BsrBI CCG'CTC
Cuts at: 50 207 582 1728 1746 2273 3983 5437 5491
Size: 157 375 1146 18 527 1710 1454 54
Cuts at: 5491 6977 7142 50
Size: 1486 165 55
Fragments arranged by size:
1710 1486 1454 1146 527 375 165 157
55 54 18
BsrDI GCAATG_nn'
Cuts at: 2863 5057 2863
Size: 2194 4953
BsrFI r'CCGG_y
Cuts at: 1942 1994 2193 2199 2656 3590 3607 5143 5324
Size: 52 199 6 457 934 17 1536 181
Cuts at: 5324 5535 1942
Size: 211 3554
Fragments arranged by size:
3554 1536 934 457 211 199 181 52
17 6
BsrGI T'GTAC_A
Cuts at: 1651 1651
Size: 7147
BssSI C'ACGA_G
Cuts at: 352 802 5416 5776 352
Size: 450 4614 360 1723
Fragments arranged by size:
4614 1723 450 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1494 4522 1494
Size: 3028 4119
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7147
BstEII G'GTnAC_C
Cuts at: 1086 1086
Size: 7147
BstXI CCAn_nnnn'nTGG
Cuts at: 2478 3042 3444 3745 2478
Size: 564 402 301 5880
Fragments arranged by size:
5880 564 402 301
BstYI r'GATC_y
Cuts at: 1 134 2447 3222 4170 4637 4660 4995 5241
Size: 133 2313 775 948 467 23 335 246
Cuts at: 5241 6255 6341 6353 6699 1
Size: 1014 86 12 346 449
Fragments arranged by size:
2313 1014 948 775 467 449 346 335
246 133 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3457 3457
Size: 7147
BtgI C'CryG_G
Cuts at: 1673 2255 2567 2698 2819 3005 3201 3379 3797
Size: 582 312 131 121 186 196 178 418
Cuts at: 3797 3822 1673
Size: 25 4998
Fragments arranged by size:
4998 582 418 312 196 186 178 131
121 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1370 1584 2751 3434 3764 3848 5095 5276 5439
Size: 214 1167 683 330 84 1247 181 163
Cuts at: 5439 1370
Size: 3078
Fragments arranged by size:
3078 1247 1167 683 330 214 181 163
84
BtsI GCAGTG_nn'
Cuts at: 258 1134 1937 3027 3765 3897 4388 258
Size: 876 803 1090 738 132 491 3017
Fragments arranged by size:
3017 1090 876 803 738 491 132
DraI TTT'AAA
Cuts at: 4144 4144
Size: 7147
DraIII CAC_nnn'GTG
Cuts at: 357 3038 357
Size: 2681 4466
DrdI GACnn_nn'nnGTC
Cuts at: 1293 4851 5711 1293
Size: 3558 860 2729
Fragments arranged by size:
3558 2729 860
EaeI y'GGCC_r
Cuts at: 840 911 971 1889 2100 2570 2654 2759 2977
Size: 71 60 918 211 470 84 105 218
Cuts at: 2977 3083 3188 3213 3770 3875 4730 4904 5295
Size: 106 105 25 557 105 855 174 391
Cuts at: 5295 5322 6669 840
Size: 27 1347 1318
Fragments arranged by size:
1347 1318 918 855 557 470 391 218
211 174 106 105 105 105 84 71
60 27 25
EagI C'GGCC_G
Cuts at: 911 971 2100 4730 911
Size: 60 1129 2630 3328
Fragments arranged by size:
3328 2630 1129 60
EarI CTCTTCn'nnn_
Cuts at: 1559 1616 1867 1875 5168 5378 1559
Size: 57 251 8 3293 210 3328
Fragments arranged by size:
3328 3293 251 210 57 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 451 738 1642 2025 3190 3430 5353 5665 5811
Size: 287 904 383 1165 240 1923 312 146
Cuts at: 5811 6957 451
Size: 1146 641
Fragments arranged by size:
1923 1165 1146 904 641 383 312 287
240 146
EcoICRI GAG'CTC
Cuts at: 288 2412 4157 288
Size: 2124 1745 3278
Fragments arranged by size:
3278 2124 1745
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 385 553 559 843 1836 1848 1860 1932
Size: 51 168 6 284 993 12 12 72
Cuts at: 1932 2142 2418 2541 3139 4970 5402 6151 6419
Size: 210 276 123 598 1831 432 749 268
Cuts at: 6419 334
Size: 1062
Fragments arranged by size:
1831 1062 993 749 598 432 284 276
268 210 168 123 72 51 12 12
6
EcoO109I rG'GnC_Cy
Cuts at: 2229 2631 3000 3182 3915 3916 4284 2229
Size: 402 369 182 733 1 368 5092
Fragments arranged by size:
5092 733 402 369 368 182 1
EcoRI G'AATT_C
Cuts at: 2005 4149 2005
Size: 2144 5003
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5164 5164
Size: 7147
FspI TGC'GCA
Cuts at: 427 1102 4926 427
Size: 675 3824 2648
Fragments arranged by size:
3824 2648 675
HaeII r_GCGC'y
Cuts at: 252 424 738 1686 1899 1959 2323 2378 3719
Size: 172 314 948 213 60 364 55 1341
Cuts at: 3719 4579 4827 5851 6830 252
Size: 860 248 1024 979 569
Fragments arranged by size:
1341 1024 979 948 860 569 364 314
248 213 172 60 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 169 196 201 228 571 603 871 903 1123
Size: 27 5 27 343 32 268 32 220
Cuts at: 1123 1155 1462 1494 1795 1827 2014 2046 2416
Size: 32 307 32 301 32 187 32 370
Cuts at: 2416 2448 3219 3251 3608 3640 4987 5017 5019
Size: 32 771 32 357 32 1347 30 2
Cuts at: 5019 5049 169
Size: 30 2267
Fragments arranged by size:
2267 1347 771 370 357 343 307 301
268 220 187 32 32 32 32 32
32 32 32 32 30 30 27 27
5 2
HincII GTy'rAC
Cuts at: 1286 1470 1835 2265 2784 4184 1286
Size: 184 365 430 519 1400 4249
Fragments arranged by size:
4249 1400 519 430 365 184
HindIII A'AGCT_T
Cuts at: 1244 1244
Size: 7147
KasI G'GCGC_C
Cuts at: 3715 4575 4823 3715
Size: 860 248 6039
Fragments arranged by size:
6039 860 248
KpnI G_GTAC'C
Cuts at: 4165 4165
Size: 7147
MluI A'CGCG_T
Cuts at: 6580 6580
Size: 7147
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 182 554 569 666 968 1490 5818 6002 182
Size: 372 15 97 302 522 4328 184 1327
Fragments arranged by size:
4328 1327 522 372 302 184 97 15
MscI TGG'CCA
Cuts at: 2761 3085 3190 3215 3877 4906 2761
Size: 324 105 25 662 1029 5002
Fragments arranged by size:
5002 1029 662 324 105 25
MslI CAynn'nnrTG
Cuts at: 634 830 1699 2179 2512 2668 2731 2824 3203
Size: 196 869 480 333 156 63 93 379
Cuts at: 3203 3739 3743 3833 4105 5134 5261 634
Size: 536 4 90 272 1029 127 2520
Fragments arranged by size:
2520 1029 869 536 480 379 333 272
196 156 127 93 90 63 4
MspA1I CmG'CkG
Cuts at: 297 393 531 624 846 1476 2148 2257 2569
Size: 96 138 93 222 630 672 109 312
Cuts at: 2569 2687 2700 2996 3275 3381 3405 3928 4256
Size: 118 13 296 279 106 24 523 328
Cuts at: 4256 4570 4930 5945 6190 297
Size: 314 360 1015 245 1254
Fragments arranged by size:
1254 1015 672 630 523 360 328 314
312 296 279 245 222 138 118 109
106 96 93 24 13
NaeI GCC'GGC
Cuts at: 1944 1996 3592 5326 1944
Size: 52 1596 1734 3765
Fragments arranged by size:
3765 1734 1596 52
NarI GG'CG_CC
Cuts at: 3716 4576 4824 3716
Size: 860 248 6039
Fragments arranged by size:
6039 860 248
NcoI C'CATG_G
Cuts at: 1673 3797 3822 1673
Size: 2124 25 4998
Fragments arranged by size:
4998 2124 25
NgoMIV G'CCGG_C
Cuts at: 1942 1994 3590 5324 1942
Size: 52 1596 1734 3765
Fragments arranged by size:
3765 1734 1596 52
NotI GC'GGCC_GC
Cuts at: 911 911
Size: 7147
NruI TCG'CGA
Cuts at: 5508 5508
Size: 7147
NspI r_CATG'y
Cuts at: 1185 3148 4198 4698 5607 1185
Size: 1963 1050 500 909 2725
Fragments arranged by size:
2725 1963 1050 909 500
PasI CC'CwG_GG
Cuts at: 1008 1227 2836 3919 1008
Size: 219 1609 1083 4236
Fragments arranged by size:
4236 1609 1083 219
PciI A'CATG_T
Cuts at: 1181 5603 1181
Size: 4422 2725
PflMI CCAn_nnn'nTGG
Cuts at: 2836 3038 3672 2836
Size: 202 634 6311
Fragments arranged by size:
6311 634 202
PfoI T'CCnGG_A
Cuts at: 1817 2606 1817
Size: 789 6358
PmeI GTTT'AAAC
Cuts at: 4144 4144
Size: 7147
PmlI CAC'GTG
Cuts at: 2085 2544 3741 2085
Size: 459 1197 5491
Fragments arranged by size:
5491 1197 459
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 310 342 6286 6318 310
Size: 32 5944 32 1139
Fragments arranged by size:
5944 1139 32 32
PpuMI rG'GwC_Cy
Cuts at: 2229 2631 3000 3182 2229
Size: 402 369 182 6194
Fragments arranged by size:
6194 402 369 182
PshAI GACnn'nnGTC
Cuts at: 1184 2102 3649 1184
Size: 918 1547 4682
Fragments arranged by size:
4682 1547 918
PspOMI G'GGCC_C
Cuts at: 2839 3915 2839
Size: 1076 6071
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1906 1938 1906
Size: 32 7115
PstI C_TGCA'G
Cuts at: 851 1004 1763 2111 4192 4877 851
Size: 153 759 348 2081 685 3121
Fragments arranged by size:
3121 2081 759 685 348 153
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7147
PvuII CAG'CTG
Cuts at: 393 531 846 1476 2148 3405 3928 4570 4930
Size: 138 315 630 672 1257 523 642 360
Cuts at: 4930 393
Size: 2610
Fragments arranged by size:
2610 1257 672 642 630 523 360 315
138
RsrII CG'GwC_CG
Cuts at: 5340 5340
Size: 7147
SacI G_AGCT'C
Cuts at: 290 2414 4159 290
Size: 2124 1745 3278
Fragments arranged by size:
3278 2124 1745
SacII CC_GC'GG
Cuts at: 2258 2570 2701 3382 2258
Size: 312 131 681 6023
Fragments arranged by size:
6023 681 312 131
SalI G'TCGA_C
Cuts at: 1284 1468 1833 4182 1284
Size: 184 365 2349 4249
Fragments arranged by size:
4249 2349 365 184
SanDI GG'GwC_CC
Cuts at: 3000 3182 3000
Size: 182 6965
SapI GCTCTTCn'nnn_
Cuts at: 1875 5168 5378 1875
Size: 3293 210 3644
Fragments arranged by size:
3644 3293 210
SbfI CC_TGCA'GG
Cuts at: 4192 4192
Size: 7147
ScaI AGT'ACT
Cuts at: 6664 6664
Size: 7147
SfcI C'TryA_G
Cuts at: 33 847 1000 1759 2107 3490 4188 4873 5868
Size: 814 153 759 348 1383 698 685 995
Cuts at: 5868 6059 33
Size: 191 1121
Fragments arranged by size:
1383 1121 995 814 759 698 685 348
191 153
SfiI GGCCn_nnn'nGGCC
Cuts at: 2815 2815
Size: 7147
SfoI GGC'GCC
Cuts at: 3717 4577 4825 3717
Size: 860 248 6039
Fragments arranged by size:
6039 860 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7147
SmaI CCC'GGG
Cuts at: 1051 2595 2636 2874 3726 4167 1051
Size: 1544 41 238 852 441 4031
Fragments arranged by size:
4031 1544 852 441 238 41
SmlI C'TyrA_G
Cuts at: 4300 5215 5709 5971 4300
Size: 915 494 262 5476
Fragments arranged by size:
5476 915 494 262
SnaBI TAC'GTA
Cuts at: 4137 4137
Size: 7147
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7147
SphI G_CATG'C
Cuts at: 4198 4698 4198
Size: 500 6647
SrfI GCCC'GGGC
Cuts at: 2874 2874
Size: 7147
StuI AGG'CCT
Cuts at: 2113 2113
Size: 7147
StyI C'CwwG_G
Cuts at: 1335 1463 1673 2405 3797 3822 4082 4279 4633
Size: 128 210 732 1392 25 260 197 354
Cuts at: 4633 1335
Size: 3849
Fragments arranged by size:
3849 1392 732 354 260 210 197 128
25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 415 718 936 1105 1823 1947 2363 3045 3420
Size: 303 218 169 718 124 416 682 375
Cuts at: 3420 3588 4728 4861 5320 5531 415
Size: 168 1140 133 459 211 2031
Fragments arranged by size:
2031 1140 718 682 459 416 375 303
218 211 169 168 133 124
TatI w'GTAC_w
Cuts at: 605 1080 1651 1736 2138 2204 6662 605
Size: 475 571 85 402 66 4458 1090
Fragments arranged by size:
4458 1090 571 475 402 85 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6664 6696 6664
Size: 32 7115
Tth111I GACn'n_nGTC
Cuts at: 2916 4942 2916
Size: 2026 5121
XbaI T'CTAG_A
Cuts at: 4176 4176
Size: 7147
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1173 1536 2475 3039 3276 4640 1173
Size: 363 939 564 237 1364 3680
Fragments arranged by size:
3680 1364 939 564 363 237
XmaI C'CCGG_G
Cuts at: 1049 2593 2634 2872 3724 4165 1049
Size: 1544 41 238 852 441 4031
Fragments arranged by size:
4031 1544 852 441 238 41
XmnI GAAnn'nnTTC
Cuts at: 754 754
Size: 7147
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AloI AscI BaeI BcgI BfrBI
BmtI BsmI BssHII BstZ17I ClaI CspCI EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NsiI PacI
PsiI PspXI SexAI SgrAI SspI SwaI XhoI ZraI