Restriction map for
pF1KSDA1064
(Circular) (Six-base) MAPSORT of: pF1KSDA1064.seq Check: 1510 from: 1 to: 7048
pF1KSDA1064 7048 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1579 1579
Size: 7048
AatII G_ACGT'C
Cuts at: 2611 2611
Size: 7048
AccI GT'mk_AC
Cuts at: 4084 4084
Size: 7048
Acc65I G'GTAC_C
Cuts at: 4062 4062
Size: 7048
AclI AA'CG_TT
Cuts at: 6888 6888
Size: 7048
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 1642 2608 2612 4006 4871 5303 6052 6320
Size: 1188 966 4 1394 865 432 749 268
Cuts at: 6320 454
Size: 1182
Fragments arranged by size:
1394 1188 1182 966 865 749 432 268
4
AflIII A'CryG_T
Cuts at: 1847 4398 5504 6481 1847
Size: 2551 1106 977 2414
Fragments arranged by size:
2551 2414 1106 977
AgeI A'CCGG_T
Cuts at: 5436 5436
Size: 7048
AhdI GACnn_n'nnGTC
Cuts at: 2982 2982
Size: 7048
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1750 3185 1750
Size: 1435 5613
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3676 3708 3676
Size: 32 7016
AlwNI CAG_nnn'CTG
Cuts at: 1833 4351 5920 1833
Size: 2518 1569 2961
Fragments arranged by size:
2961 2518 1569
ApaI G_GGCC'C
Cuts at: 1787 1966 1973 2092 2122 2369 2430 2860 2919
Size: 179 7 119 30 247 61 430 59
Cuts at: 2919 3185 1787
Size: 266 5650
Fragments arranged by size:
5650 430 266 247 179 119 61 59
30 7
ApaLI G'TGCA_C
Cuts at: 3865 5818 3865
Size: 1953 5095
ApoI r'AATT_y
Cuts at: 4050 6401 6657 4050
Size: 2351 256 4441
Fragments arranged by size:
4441 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7048
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7048
AvaI C'yCGr_G
Cuts at: 139 1121 1780 2959 3067 3204 3338 3401 3450
Size: 982 659 1179 108 137 134 63 49
Cuts at: 3450 3576 3838 3859 4066 139
Size: 126 262 21 207 3121
Fragments arranged by size:
3121 1179 982 659 262 207 137 134
126 108 63 49 21
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2532 2565 2532
Size: 33 7015
BamHI G'GATC_C
Cuts at: 2441 4071 2441
Size: 1630 5418
BanI G'GyrC_C
Cuts at: 1591 2389 2963 3208 3235 3429 3628 3751 3844
Size: 798 574 245 27 194 199 123 93
Cuts at: 3844 4062 4476 4724 4759 1591
Size: 218 414 248 35 3880
Fragments arranged by size:
3880 798 574 414 248 245 218 199
194 123 93 35 27
BanII G_rGCy'C
Cuts at: 1120 1556 1787 1966 1973 2092 2122 2252 2345
Size: 436 231 179 7 119 30 130 93
Cuts at: 2345 2369 2430 2860 2919 3185 3667 3689 3925
Size: 24 61 430 59 266 482 22 236
Cuts at: 3925 4060 5090 1120
Size: 135 1030 3078
Fragments arranged by size:
3078 1030 482 436 430 266 236 231
179 135 130 119 93 61 59 30
24 22 7
BbeI G_GCGC'C
Cuts at: 2393 3212 3239 3433 4480 4728 2393
Size: 819 27 194 1047 248 4713
Fragments arranged by size:
4713 1047 819 248 194 27
BbsI GAAGACnn'nnnn_
Cuts at: 1214 2596 1214
Size: 1382 5666
BbvCI CC'TCA_GC
Cuts at: 2415 2716 3425 3647 2415
Size: 301 709 222 5816
Fragments arranged by size:
5816 709 301 222
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1302 1336 1379 1413 1302
Size: 34 43 34 6937
Fragments arranged by size:
6937 43 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 339 4944 5707 7048 339
Size: 4605 763 1341 339
Fragments arranged by size:
4605 1341 763 339
BclI T'GATC_A
Cuts at: 4566 4566
Size: 7048
BfrBI ATG'CAT
Cuts at: 2010 2010
Size: 7048
BglI GCCn_nnn'nGGC
Cuts at: 238 1163 2126 2425 2855 3676 238
Size: 925 963 299 430 821 3610
Fragments arranged by size:
3610 963 925 821 430 299
BglII A'GATC_T
Cuts at: 4561 6600 4561
Size: 2039 5009
BlpI GC'TnA_GC
Cuts at: 2641 3553 4158 2641
Size: 912 605 5531
Fragments arranged by size:
5531 912 605
Bme1580I G_kGCm'C
Cuts at: 1787 1966 1973 2092 2122 2285 2369 2430 2860
Size: 179 7 119 30 163 84 61 430
Cuts at: 2860 2919 2966 3113 3122 3185 3633 3754 3849
Size: 59 47 147 9 63 448 121 95
Cuts at: 3849 3869 3884 4671 4764 5822 1787
Size: 20 15 787 93 1058 3013
Fragments arranged by size:
3013 1058 787 448 430 179 163 147
121 119 95 93 84 63 61 59
47 30 20 15 9 7
BmgBI CAC'GTC
Cuts at: 3021 3021
Size: 7048
BmrI ACTGGGnnnn_n'
Cuts at: 560 1496 3323 3526 4258 4339 4674 6820 560
Size: 936 1827 203 732 81 335 2146 788
Fragments arranged by size:
2146 1827 936 788 732 335 203 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3450 5413 3450
Size: 1963 5085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 2359 2656 2174
Size: 185 297 6566
Fragments arranged by size:
6566 297 185
Bpu10I CC'TnA_GC
Cuts at: 2327 2415 2716 3028 3425 3647 3680 3924 2327
Size: 88 301 312 397 222 33 244 5451
Fragments arranged by size:
5451 397 312 301 244 222 88 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2529 2616 3259 4222 5101 5595 5893 2529
Size: 87 643 963 879 494 298 3684
Fragments arranged by size:
3684 963 879 643 494 298 87
BsaI GGTCTCn'nnnn_
Cuts at: 331 944 1340 6752 331
Size: 613 396 5412 627
Fragments arranged by size:
5412 627 613 396
BsaAI yAC'GTr
Cuts at: 4038 4399 4038
Size: 361 6687
BsaBI GATnn'nnATC
Cuts at: 6 4560 6599 6
Size: 4554 2039 455
Fragments arranged by size:
4554 2039 455
BsaHI Gr'CG_yC
Cuts at: 230 2390 2608 3209 3236 3430 4477 4725 5429
Size: 2160 218 601 27 194 1047 248 704
Cuts at: 5429 6925 230
Size: 1496 353
Fragments arranged by size:
2160 1496 1047 704 601 353 248 218
194 27
BsaWI w'CCGG_w
Cuts at: 352 1253 2530 4235 4756 5436 5710 5857 6268
Size: 901 1277 1705 521 680 274 147 411
Cuts at: 6268 352
Size: 1132
Fragments arranged by size:
1705 1277 1132 901 680 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1027 1054 1057 1084 3676 3706 1027
Size: 27 3 27 2592 30 4369
Fragments arranged by size:
4369 2592 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 203 244 349 427 501 763 766 962 1024
Size: 41 105 78 74 262 3 196 62
Cuts at: 1024 1187 1676 2476 2479 2482 2493 2911 2914
Size: 163 489 800 3 3 11 418 3
Cuts at: 2914 3288 3387 203
Size: 374 99 3864
Fragments arranged by size:
3864 800 489 418 374 262 196 163
105 99 78 74 62 41 11 3
3 3 3
BseYI C'CCAG_C
Cuts at: 1917 2655 2860 3037 3632 3854 3940 4471 5229
Size: 738 205 177 595 222 86 531 758
Cuts at: 5229 5808 6304 1917
Size: 579 496 2661
Fragments arranged by size:
2661 758 738 595 579 531 496 222
205 177 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1320 3145 1320
Size: 1825 5223
BsiEI CG_ry'CG
Cuts at: 104 837 2380 3305 3509 4588 4634 5844 104
Size: 733 1543 925 204 1079 46 1210 1308
Fragments arranged by size:
1543 1308 1210 1079 925 733 204 46
BsiHKAI G_wGCw'C
Cuts at: 1556 2318 2333 3059 3667 3869 4060 4838 5028
Size: 762 15 726 608 202 191 778 190
Cuts at: 5028 5822 1556
Size: 794 2782
Fragments arranged by size:
2782 794 778 762 726 608 202 191
190 15
BsmBI CGTCTCn'nnnn_
Cuts at: 1016 1537 1016
Size: 521 6527
Bsp1286I G_dGCh'C
Cuts at: 1120 1556 1787 1966 1973 2092 2122 2252 2285
Size: 436 231 179 7 119 30 130 33
Cuts at: 2285 2318 2333 2345 2369 2430 2860 2919 2966
Size: 33 15 12 24 61 430 59 47
Cuts at: 2966 3059 3113 3122 3185 3633 3667 3689 3754
Size: 93 54 9 63 448 34 22 65
Cuts at: 3754 3849 3869 3884 3925 4060 4671 4764 4838
Size: 95 20 15 41 135 611 93 74
Cuts at: 4838 5028 5090 5822 1120
Size: 190 62 732 2346
Fragments arranged by size:
2346 732 611 448 436 430 231 190
179 135 130 119 95 93 93 74
65 63 62 61 59 54 47 41
34 33 33 30 24 22 20 15
15 12 9 7
BspEI T'CCGG_A
Cuts at: 352 6268 352
Size: 5916 1132
BspHI T'CATG_A
Cuts at: 6224 6224
Size: 7048
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1579 3881 4096 4310 4612 4993 1579
Size: 2302 215 214 302 381 3634
Fragments arranged by size:
3634 2302 381 302 215 214
BsrBI CCG'CTC
Cuts at: 50 273 456 1070 2913 3481 3557 5338 5392
Size: 223 183 614 1843 568 76 1781 54
Cuts at: 5392 6878 7043 50
Size: 1486 165 55
Fragments arranged by size:
1843 1781 1486 614 568 223 183 165
76 55 54
BsrDI GCAATG_nn'
Cuts at: 3660 4958 3660
Size: 1298 5750
BsrFI r'CCGG_y
Cuts at: 806 1803 2375 2392 2696 3292 3508 3612 3736
Size: 997 572 17 304 596 216 104 124
Cuts at: 3736 3782 5044 5225 5436 806
Size: 46 1262 181 211 2418
Fragments arranged by size:
2418 1262 997 596 572 304 216 211
181 124 104 46 17
BsrGI T'GTAC_A
Cuts at: 1850 1850
Size: 7048
BssSI C'ACGA_G
Cuts at: 5317 5677 5317
Size: 360 6688
BstAPI GCAn_nnn'nTGC
Cuts at: 1833 2970 3138 3873 4423 1833
Size: 1137 168 735 550 4458
Fragments arranged by size:
4458 1137 735 550 168
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7048
BstEII G'GTnAC_C
Cuts at: 577 577
Size: 7048
BstXI CCAn_nnnn'nTGG
Cuts at: 1486 2853 1486
Size: 1367 5681
BstYI r'GATC_y
Cuts at: 1 1862 2441 4071 4538 4561 4896 5142 6156
Size: 1861 579 1630 467 23 335 246 1014
Cuts at: 6156 6242 6254 6600 1
Size: 86 12 346 449
Fragments arranged by size:
1861 1630 1014 579 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2437 2437
Size: 7048
BtgI C'CryG_G
Cuts at: 125 831 882 900 1107 1895 2114 2980 3122
Size: 706 51 18 207 788 219 866 142
Cuts at: 3122 3173 3215 3758 4013 125
Size: 51 42 543 255 3160
Fragments arranged by size:
3160 866 788 706 543 255 219 207
142 51 51 42 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 167 539 623 4996 5177 5340 167
Size: 372 84 4373 181 163 1875
Fragments arranged by size:
4373 1875 372 181 163 84
BtsI GCAGTG_nn'
Cuts at: 2577 2655 3113 3552 4289 2577
Size: 78 458 439 737 5336
Fragments arranged by size:
5336 737 458 439 78
DraI TTT'AAA
Cuts at: 3999 4045 3999
Size: 46 7002
DrdI GACnn_nn'nnGTC
Cuts at: 4752 5612 4752
Size: 860 6188
EaeI y'GGCC_r
Cuts at: 239 834 1571 2377 3302 3506 4631 4805 5196
Size: 595 737 806 925 204 1125 174 391
Cuts at: 5196 5223 6570 239
Size: 27 1347 717
Fragments arranged by size:
1347 1125 925 806 737 717 595 391
204 174 27
EagI C'GGCC_G
Cuts at: 834 2377 3302 3506 4631 834
Size: 1543 925 204 1125 3251
Fragments arranged by size:
3251 1543 1125 925 204
EarI CTCTTCn'nnn_
Cuts at: 478 962 980 1535 2285 2326 3895 5069 5279
Size: 484 18 555 750 41 1569 1174 210
Cuts at: 5279 478
Size: 2247
Fragments arranged by size:
2247 1569 1174 750 555 484 210 41
18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1205 3542 5254 5566 5712 6858 1205
Size: 2337 1712 312 146 1146 1395
Fragments arranged by size:
2337 1712 1395 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1554 3665 4058 1554
Size: 2111 393 4544
Fragments arranged by size:
4544 2111 393
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 1642 2174 2359 2608 2612 2656 4006 4871
Size: 1188 532 185 249 4 44 1350 865
Cuts at: 4871 5303 6052 6320 454
Size: 432 749 268 1182
Fragments arranged by size:
1350 1188 1182 865 749 532 432 268
249 185 44 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2674 2674
Size: 7048
EcoO109I rG'GnC_Cy
Cuts at: 143 358 1784 1963 1981 2089 2119 2365 2366
Size: 215 1426 179 18 108 30 246 1
Cuts at: 2366 2426 2427 2661 2724 2782 2857 2916 2976
Size: 60 1 234 63 58 75 59 60
Cuts at: 2976 3072 3181 3182 3351 4185 143
Size: 96 109 1 169 834 3006
Fragments arranged by size:
3006 1426 834 246 234 215 179 169
109 108 96 75 63 60 60 59
58 30 18 1 1 1
EcoRI G'AATT_C
Cuts at: 4050 4050
Size: 7048
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2854 3057 5065 2854
Size: 203 2008 4837
Fragments arranged by size:
4837 2008 203
FseI GG_CCGG'CC
Cuts at: 2379 2379
Size: 7048
FspI TGC'GCA
Cuts at: 4827 4827
Size: 7048
HaeII r_GCGC'y
Cuts at: 1335 2393 3204 3212 3239 3433 4480 4728 5752
Size: 1058 811 8 27 194 1047 248 1024
Cuts at: 5752 6731 1335
Size: 979 1652
Fragments arranged by size:
1652 1058 1047 1024 979 811 248 194
27 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 403 435 517 549 1027 1059 1804 1836 2338
Size: 32 82 32 478 32 745 32 502
Cuts at: 2338 2370 2968 3000 3964 3996 4888 4918 4920
Size: 32 598 32 964 32 892 30 2
Cuts at: 4920 4950 403
Size: 30 2501
Fragments arranged by size:
2501 964 892 745 598 502 478 82
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 4085 4085
Size: 7048
KasI G'GCGC_C
Cuts at: 2389 3208 3235 3429 4476 4724 2389
Size: 819 27 194 1047 248 4713
Fragments arranged by size:
4713 1047 819 248 194 27
KpnI G_GTAC'C
Cuts at: 4066 4066
Size: 7048
MluI A'CGCG_T
Cuts at: 6481 6481
Size: 7048
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 281 1268 1735 5719 5903 281
Size: 987 467 3984 184 1426
Fragments arranged by size:
3984 1426 987 467 184
MscI TGG'CCA
Cuts at: 4807 4807
Size: 7048
MslI CAynn'nnrTG
Cuts at: 398 1804 2083 2242 5035 5162 398
Size: 1406 279 159 2793 127 2284
Fragments arranged by size:
2793 2284 1406 279 159 127
MspA1I CmG'CkG
Cuts at: 833 902 1833 1905 2991 3124 3814 4157 4471
Size: 69 931 72 1086 133 690 343 314
Cuts at: 4471 4831 5846 6091 833
Size: 360 1015 245 1790
Fragments arranged by size:
1790 1086 1015 931 690 360 343 314
245 133 72 69
NaeI GCC'GGC
Cuts at: 2377 2698 3614 5227 2377
Size: 321 916 1613 4198
Fragments arranged by size:
4198 1613 916 321
NarI GG'CG_CC
Cuts at: 2390 3209 3236 3430 4477 4725 2390
Size: 819 27 194 1047 248 4713
Fragments arranged by size:
4713 1047 819 248 194 27
NcoI C'CATG_G
Cuts at: 125 2114 125
Size: 1989 5059
NdeI CA'TA_TG
Cuts at: 609 609
Size: 7048
NgoMIV G'CCGG_C
Cuts at: 2375 2696 3612 5225 2375
Size: 321 916 1613 4198
Fragments arranged by size:
4198 1613 916 321
NruI TCG'CGA
Cuts at: 894 5409 894
Size: 4515 2533
NsiI A_TGCA'T
Cuts at: 2012 2012
Size: 7048
NspI r_CATG'y
Cuts at: 646 702 1518 1851 2022 2034 2046 2058 2070
Size: 56 816 333 171 12 12 12 12
Cuts at: 2070 2082 4099 4599 5508 646
Size: 12 2017 500 909 2186
Fragments arranged by size:
2186 2017 909 816 500 333 171 56
12 12 12 12 12
PasI CC'CwG_GG
Cuts at: 1951 1966 1987 1951
Size: 15 21 7012
Fragments arranged by size:
7012 21 15
PciI A'CATG_T
Cuts at: 1847 5504 1847
Size: 3657 3391
PflMI CCAn_nnn'nTGG
Cuts at: 2170 3940 2170
Size: 1770 5278
PmeI GTTT'AAAC
Cuts at: 4045 4045
Size: 7048
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3676 3708 6187 6219 3676
Size: 32 2479 32 4505
Fragments arranged by size:
4505 2479 32 32
PpuMI rG'GwC_Cy
Cuts at: 143 1981 2661 2724 2782 2976 3072 143
Size: 1838 680 63 58 194 96 4119
Fragments arranged by size:
4119 1838 680 194 96 63 58
PspOMI G'GGCC_C
Cuts at: 1783 1962 1969 2088 2118 2365 2426 2856 2915
Size: 179 7 119 30 247 61 430 59
Cuts at: 2915 3181 1783
Size: 266 5650
Fragments arranged by size:
5650 430 266 247 179 119 61 59
30 7
PstI C_TGCA'G
Cuts at: 1838 2996 3374 3807 4093 4778 1838
Size: 1158 378 433 286 685 4108
Fragments arranged by size:
4108 1158 685 433 378 286
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7048
PvuII CAG'CTG
Cuts at: 1833 1905 2991 4471 4831 1833
Size: 72 1086 1480 360 4050
Fragments arranged by size:
4050 1480 1086 360 72
RsrII CG'GwC_CG
Cuts at: 5241 5241
Size: 7048
SacI G_AGCT'C
Cuts at: 1556 3667 4060 1556
Size: 2111 393 4544
Fragments arranged by size:
4544 2111 393
SacII CC_GC'GG
Cuts at: 834 903 3125 834
Size: 69 2222 4757
Fragments arranged by size:
4757 2222 69
SalI G'TCGA_C
Cuts at: 4083 4083
Size: 7048
SanDI GG'GwC_CC
Cuts at: 143 1981 2661 2724 2782 2976 143
Size: 1838 680 63 58 194 4215
Fragments arranged by size:
4215 1838 680 194 63 58
SapI GCTCTTCn'nnn_
Cuts at: 2285 5069 5279 2285
Size: 2784 210 4054
Fragments arranged by size:
4054 2784 210
SbfI CC_TGCA'GG
Cuts at: 4093 4093
Size: 7048
ScaI AGT'ACT
Cuts at: 1042 1318 6565 1042
Size: 276 5247 1525
Fragments arranged by size:
5247 1525 276
SfcI C'TryA_G
Cuts at: 33 864 1834 2992 3370 3803 4089 4774 5769
Size: 831 970 1158 378 433 286 685 995
Cuts at: 5769 5960 33
Size: 191 1121
Fragments arranged by size:
1158 1121 995 970 831 685 433 378
286 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2126 2126
Size: 7048
SfoI GGC'GCC
Cuts at: 2391 3210 3237 3431 4478 4726 2391
Size: 819 27 194 1047 248 4713
Fragments arranged by size:
4713 1047 819 248 194 27
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7048
SgrAI Cr'CCGG_yG
Cuts at: 2392 2392
Size: 7048
SmaI CCC'GGG
Cuts at: 141 1782 2961 3206 3340 3403 3861 4068 141
Size: 1641 1179 245 134 63 458 207 3121
Fragments arranged by size:
3121 1641 1179 458 245 207 134 63
SmlI C'TyrA_G
Cuts at: 2544 2595 3274 4201 5116 5610 5872 2544
Size: 51 679 927 915 494 262 3720
Fragments arranged by size:
3720 927 915 679 494 262 51
SnaBI TAC'GTA
Cuts at: 4038 4038
Size: 7048
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7048
SphI G_CATG'C
Cuts at: 646 4099 4599 646
Size: 3453 500 3095
Fragments arranged by size:
3453 3095 500
SrfI GCCC'GGGC
Cuts at: 1782 3206 1782
Size: 1424 5624
StyI C'CwwG_G
Cuts at: 125 815 1046 1073 2114 2870 3638 3674 3794
Size: 690 231 27 1041 756 768 36 120
Cuts at: 3794 4180 4534 125
Size: 386 354 2639
Fragments arranged by size:
2639 1041 768 756 690 386 354 231
120 36 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1552 1909 2127 3927 4629 4762 5221 5432 1552
Size: 357 218 1800 702 133 459 211 3168
Fragments arranged by size:
3168 1800 702 459 357 218 211 133
TatI w'GTAC_w
Cuts at: 587 596 1040 1316 1850 6563 587
Size: 9 444 276 534 4713 1072
Fragments arranged by size:
4713 1072 534 444 276 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3723 3755 6565 6597 3723
Size: 32 2810 32 4174
Fragments arranged by size:
4174 2810 32 32
Tth111I GACn'n_nGTC
Cuts at: 4843 4843
Size: 7048
XbaI T'CTAG_A
Cuts at: 4077 4077
Size: 7048
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1902 3144 4541 1902
Size: 1242 1397 4409
Fragments arranged by size:
4409 1397 1242
XmaI C'CCGG_G
Cuts at: 139 1780 2959 3204 3338 3401 3859 4066 139
Size: 1641 1179 245 134 63 458 207 3121
Fragments arranged by size:
3121 1641 1179 458 245 207 134 63
ZraI GAC'GTC
Cuts at: 2609 2609
Size: 7048
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NruI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AleI AscI AvrII BmtI BsiWI BsmI
BssHII BstZ17I ClaI CspCI DraIII EcoRV FspAI HindIII
HpaI MfeI NheI NotI PacI PfoI PmlI PshAI
PsiI PspXI PsrI SexAI SspI StuI SwaI XhoI
XmnI