Restriction map for
pF1KSDA1056
(Circular) (Six-base) MAPSORT of: pF1KSDA1056.seq Check: 8915 from: 1 to: 5723
pF1KSDA1056 5723 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2151 2204 2507 2594 2151
Size: 53 303 87 5280
Fragments arranged by size:
5280 303 87 53
AatII G_ACGT'C
Cuts at: 1661 1661
Size: 5723
AccI GT'mk_AC
Cuts at: 2761 2761
Size: 5723
Acc65I G'GTAC_C
Cuts at: 157 2739 157
Size: 2582 3141
AclI AA'CG_TT
Cuts at: 5563 5563
Size: 5723
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2289 2293 3548 3980 4727 4995 2289
Size: 4 1255 432 747 268 3017
Fragments arranged by size:
3017 1255 747 432 268 4
AflIII A'CryG_T
Cuts at: 1445 3075 4179 5156 1445
Size: 1630 1104 977 2012
Fragments arranged by size:
2012 1630 1104 977
AgeI A'CCGG_T
Cuts at: 4111 4111
Size: 5723
AhdI GACnn_n'nnGTC
Cuts at: 2265 2610 2265
Size: 345 5378
AleI CACnn'nnGTG
Cuts at: 1485 1485
Size: 5723
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1720 2657 1720
Size: 937 4786
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 265 297 265
Size: 32 5691
AlwNI CAG_nnn'CTG
Cuts at: 306 936 1411 1527 1571 1580 1935 2214 2695
Size: 630 475 116 44 9 355 279 481
Cuts at: 2695 3028 4595 306
Size: 333 1567 1434
Fragments arranged by size:
1567 1434 630 481 475 355 333 279
116 44 9
ApaI G_GGCC'C
Cuts at: 1753 2036 1753
Size: 283 5440
ApaLI G'TGCA_C
Cuts at: 1534 4493 1534
Size: 2959 2764
ApoI r'AATT_y
Cuts at: 683 2727 5076 5332 683
Size: 2044 2349 256 1074
Fragments arranged by size:
2349 2044 1074 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5723
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5723
AvaI C'yCGr_G
Cuts at: 2073 2743 2073
Size: 670 5053
AvrII C'CTAG_G
Cuts at: 2043 2043
Size: 5723
BamHI G'GATC_C
Cuts at: 2748 2748
Size: 5723
BanI G'GyrC_C
Cuts at: 157 1594 2422 2524 2739 3153 3401 3436 157
Size: 1437 828 102 215 414 248 35 2444
Fragments arranged by size:
2444 1437 828 414 248 215 102 35
BanII G_rGCy'C
Cuts at: 353 440 584 1280 1348 1753 2036 2559 2737
Size: 87 144 696 68 405 283 523 178
Cuts at: 2737 3767 353
Size: 1030 2309
Fragments arranged by size:
2309 1030 696 523 405 283 178 144
87 68
BbeI G_GCGC'C
Cuts at: 1598 3157 3405 1598
Size: 1559 248 3916
Fragments arranged by size:
3916 1559 248
BbsI GAAGACnn'nnnn_
Cuts at: 822 1361 822
Size: 539 5184
BbvCI CC'TCA_GC
Cuts at: 702 1153 1429 1693 2080 702
Size: 451 276 264 387 4345
Fragments arranged by size:
4345 451 387 276 264
BciVI GTATCCnnnnn_n'
Cuts at: 3621 4382 5723 3621
Size: 761 1341 3621
Fragments arranged by size:
3621 1341 761
BclI T'GATC_A
Cuts at: 3243 3243
Size: 5723
BglII A'GATC_T
Cuts at: 3238 5275 3238
Size: 2037 3686
BlpI GC'TnA_GC
Cuts at: 2835 2835
Size: 5723
Bme1580I G_kGCm'C
Cuts at: 179 1327 1538 1753 2036 2527 3348 3441 4497
Size: 1148 211 215 283 491 821 93 1056
Cuts at: 4497 179
Size: 1405
Fragments arranged by size:
1405 1148 1056 821 491 283 215 211
93
BmrI ACTGGGnnnn_n'
Cuts at: 578 1333 1355 2935 3016 3351 5495 578
Size: 755 22 1580 81 335 2144 806
Fragments arranged by size:
2144 1580 806 755 335 81 22
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4090 4090
Size: 5723
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 328 500 768 943 1021 2341 198
Size: 130 172 268 175 78 1320 3580
Fragments arranged by size:
3580 1320 268 175 172 130 78
Bpu10I CC'TnA_GC
Cuts at: 454 702 1153 1429 1693 2080 454
Size: 248 451 276 264 387 4097
Fragments arranged by size:
4097 451 387 276 264 248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1468 2416 2440 2899 3778 4270 4568 1468
Size: 948 24 459 879 492 298 2623
Fragments arranged by size:
2623 948 879 492 459 298 24
BsaI GGTCTCn'nnnn_
Cuts at: 5427 5427
Size: 5723
BsaAI yAC'GTr
Cuts at: 822 2133 2715 3076 822
Size: 1311 582 361 3469
Fragments arranged by size:
3469 1311 582 361
BsaBI GATnn'nnATC
Cuts at: 6 3237 5274 6
Size: 3231 2037 455
Fragments arranged by size:
3231 2037 455
BsaHI Gr'CG_yC
Cuts at: 1595 1658 3154 3402 4104 5600 1595
Size: 63 1496 248 702 1496 1718
Fragments arranged by size:
1718 1496 1496 702 248 63
BsaWI w'CCGG_w
Cuts at: 2912 3433 4111 4385 4532 4943 2912
Size: 521 678 274 147 411 3692
Fragments arranged by size:
3692 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 193 223 193
Size: 30 5693
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 201 249 436 1967 201
Size: 48 187 1531 3957
Fragments arranged by size:
3957 1531 187 48
BseYI C'CCAG_C
Cuts at: 948 1189 1373 1404 1588 1941 2105 2426 2535
Size: 241 184 31 184 353 164 321 109
Cuts at: 2535 3148 3906 4483 4979 948
Size: 613 758 577 496 1692
Fragments arranged by size:
1692 758 613 577 496 353 321 241
184 184 164 109 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1866 1909 2672 1866
Size: 43 763 4917
Fragments arranged by size:
4917 763 43
BsiEI CG_ry'CG
Cuts at: 104 3265 3311 4519 104
Size: 3161 46 1208 1308
Fragments arranged by size:
3161 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1538 2142 2737 3515 3705 4497 1538
Size: 604 595 778 190 792 2764
Fragments arranged by size:
2764 792 778 604 595 190
Bsp1286I G_dGCh'C
Cuts at: 179 353 440 584 1280 1327 1348 1538 1753
Size: 174 87 144 696 47 21 190 215
Cuts at: 1753 2036 2142 2527 2559 2737 3348 3441 3515
Size: 283 106 385 32 178 611 93 74
Cuts at: 3515 3705 3767 4497 179
Size: 190 62 730 1405
Fragments arranged by size:
1405 730 696 611 385 283 215 190
190 178 174 144 106 93 87 74
62 47 32 21
BspEI T'CCGG_A
Cuts at: 4943 4943
Size: 5723
BspHI T'CATG_A
Cuts at: 4899 4899
Size: 5723
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1883 2151 2204 2507 2594 2773 2987 3289 3670
Size: 268 53 303 87 179 214 302 381
Cuts at: 3670 1883
Size: 3936
Fragments arranged by size:
3936 381 303 302 268 214 179 87
53
BsrBI CCG'CTC
Cuts at: 50 4015 4069 5553 5718 50
Size: 3965 54 1484 165 55
Fragments arranged by size:
3965 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3635 3635
Size: 5723
BsrFI r'CCGG_y
Cuts at: 3721 3902 4111 3721
Size: 181 209 5333
Fragments arranged by size:
5333 209 181
BssSI C'ACGA_G
Cuts at: 1613 3994 4352 1613
Size: 2381 358 2984
Fragments arranged by size:
2984 2381 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1472 1580 1844 2411 3100 1472
Size: 108 264 567 689 4095
Fragments arranged by size:
4095 689 567 264 108
BstBI TT'CG_AA
Cuts at: 110 4084 110
Size: 3974 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2029
Size: 5723
BstYI r'GATC_y
Cuts at: 1 243 780 2748 3215 3238 3573 3819 4831
Size: 242 537 1968 467 23 335 246 1012
Cuts at: 4831 4917 4929 5275 1
Size: 86 12 346 449
Fragments arranged by size:
1968 1012 537 467 449 346 335 246
242 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1103 1253 1103
Size: 150 5573
BtgI C'CryG_G
Cuts at: 125 446 797 1796 2519 125
Size: 321 351 999 723 3329
Fragments arranged by size:
3329 999 723 351 321
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3673 3854 4017 3673
Size: 181 163 5379
Fragments arranged by size:
5379 181 163
BtsI GCAGTG_nn'
Cuts at: 531 623 1569 1590 1840 2337 2966 531
Size: 92 946 21 250 497 629 3288
Fragments arranged by size:
3288 946 629 497 250 92 21
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1940 1975 1940
Size: 35 5688
DraI TTT'AAA
Cuts at: 2722 2722
Size: 5723
DrdI GACnn_nn'nnGTC
Cuts at: 3429 4287 3429
Size: 858 4865
EaeI y'GGCC_r
Cuts at: 128 500 1724 3308 3482 3873 3900 5245 128
Size: 372 1224 1584 174 391 27 1345 606
Fragments arranged by size:
1584 1345 1224 606 391 372 174 27
EagI C'GGCC_G
Cuts at: 3308 3308
Size: 5723
EarI CTCTTCn'nnn_
Cuts at: 572 678 1032 1259 2023 3746 3956 572
Size: 106 354 227 764 1723 210 2339
Fragments arranged by size:
2339 1723 764 354 227 210 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2154 3931 4241 4387 5533 2154
Size: 1777 310 146 1146 2344
Fragments arranged by size:
2344 1777 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2735 2735
Size: 5723
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 328 500 768 943 1021 2289 2293 2341
Size: 130 172 268 175 78 1268 4 48
Cuts at: 2341 3548 3980 4727 4995 198
Size: 1207 432 747 268 926
Fragments arranged by size:
1268 1207 926 747 432 268 268 175
172 130 78 48 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2503 2503
Size: 5723
EcoO109I rG'GnC_Cy
Cuts at: 281 361 933 984 1119 1185 1425 1750 1972
Size: 80 572 51 135 66 240 325 222
Cuts at: 1972 2033 2040 2862 281
Size: 61 7 822 3142
Fragments arranged by size:
3142 822 572 325 240 222 135 80
66 61 51 7
EcoRI G'AATT_C
Cuts at: 2727 2727
Size: 5723
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2457 3742 2457
Size: 1285 4438
FspI TGC'GCA
Cuts at: 1879 3504 1879
Size: 1625 4098
HaeII r_GCGC'y
Cuts at: 1598 3157 3405 4427 5406 1598
Size: 1559 248 1022 979 1915
Fragments arranged by size:
1915 1559 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 193 225 1551 1583 2251 2283 2596 2628 3565
Size: 32 1326 32 668 32 313 32 937
Cuts at: 3565 3595 3597 3627 193
Size: 30 2 30 2289
Fragments arranged by size:
2289 1326 937 668 313 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2762 2762
Size: 5723
HindIII A'AGCT_T
Cuts at: 993 2407 993
Size: 1414 4309
KasI G'GCGC_C
Cuts at: 1594 3153 3401 1594
Size: 1559 248 3916
Fragments arranged by size:
3916 1559 248
KpnI G_GTAC'C
Cuts at: 161 2743 161
Size: 2582 3141
MluI A'CGCG_T
Cuts at: 5156 5156
Size: 5723
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 663 1795 2469 2639 4394 4578 663
Size: 1132 674 170 1755 184 1808
Fragments arranged by size:
1808 1755 1132 674 184 170
MscI TGG'CCA
Cuts at: 130 502 1726 3484 130
Size: 372 1224 1758 2369
Fragments arranged by size:
2369 1758 1224 372
MslI CAynn'nnrTG
Cuts at: 1485 1702 1885 3712 3839 1485
Size: 217 183 1827 127 3369
Fragments arranged by size:
3369 1827 217 183 127
MspA1I CmG'CkG
Cuts at: 478 759 809 952 1652 1798 1941 2285 2402
Size: 281 50 143 700 146 143 344 117
Cuts at: 2402 2487 2637 2834 3148 3508 4521 4766 478
Size: 85 150 197 314 360 1013 245 1435
Fragments arranged by size:
1435 1013 700 360 344 314 281 245
197 150 146 143 143 117 85 50
NaeI GCC'GGC
Cuts at: 3904 3904
Size: 5723
NarI GG'CG_CC
Cuts at: 1595 3154 3402 1595
Size: 1559 248 3916
Fragments arranged by size:
3916 1559 248
NcoI C'CATG_G
Cuts at: 125 446 797 2519 125
Size: 321 351 1722 3329
Fragments arranged by size:
3329 1722 351 321
NdeI CA'TA_TG
Cuts at: 1703 1886 1703
Size: 183 5540
NgoMIV G'CCGG_C
Cuts at: 3902 3902
Size: 5723
NspI r_CATG'y
Cuts at: 1449 1542 1908 2473 2644 2776 3276 4183 1449
Size: 93 366 565 171 132 500 907 2989
Fragments arranged by size:
2989 907 565 500 366 171 132 93
PasI CC'CwG_GG
Cuts at: 172 394 554 2544 172
Size: 222 160 1990 3351
Fragments arranged by size:
3351 1990 222 160
PciI A'CATG_T
Cuts at: 1445 4179 1445
Size: 2734 2989
PfoI T'CCnGG_A
Cuts at: 305 959 1130 2360 305
Size: 654 171 1230 3668
Fragments arranged by size:
3668 1230 654 171
PmeI GTTT'AAAC
Cuts at: 2722 2722
Size: 5723
PmlI CAC'GTG
Cuts at: 822 822
Size: 5723
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4862 4894 4862
Size: 32 5691
PpuMI rG'GwC_Cy
Cuts at: 281 1185 281
Size: 904 4819
PshAI GACnn'nnGTC
Cuts at: 371 371
Size: 5723
PspOMI G'GGCC_C
Cuts at: 1749 2032 1749
Size: 283 5440
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 521 553 521
Size: 32 5691
PstI C_TGCA'G
Cuts at: 1416 1576 1585 2344 2770 3455 1416
Size: 160 9 759 426 685 3684
Fragments arranged by size:
3684 759 685 426 160 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5723
PvuII CAG'CTG
Cuts at: 478 759 952 2487 2637 3148 3508 478
Size: 281 193 1535 150 511 360 2693
Fragments arranged by size:
2693 1535 511 360 281 193 150
RsrII CG'GwC_CG
Cuts at: 3918 3918
Size: 5723
SacI G_AGCT'C
Cuts at: 2737 2737
Size: 5723
SacII CC_GC'GG
Cuts at: 1799 1799
Size: 5723
SalI G'TCGA_C
Cuts at: 2760 2760
Size: 5723
SanDI GG'GwC_CC
Cuts at: 1185 1185
Size: 5723
SapI GCTCTTCn'nnn_
Cuts at: 572 678 3746 3956 572
Size: 106 3068 210 2339
Fragments arranged by size:
3068 2339 210 106
SbfI CC_TGCA'GG
Cuts at: 2770 2770
Size: 5723
ScaI AGT'ACT
Cuts at: 2026 5240 2026
Size: 3214 2509
SfcI C'TryA_G
Cuts at: 33 1412 1572 1581 1732 2340 2766 3451 4444
Size: 1379 160 9 151 608 426 685 993
Cuts at: 4444 4635 33
Size: 191 1121
Fragments arranged by size:
1379 1121 993 685 608 426 191 160
151 9
SfoI GGC'GCC
Cuts at: 1596 3155 3403 1596
Size: 1559 248 3916
Fragments arranged by size:
3916 1559 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5723
SmaI CCC'GGG
Cuts at: 2075 2745 2075
Size: 670 5053
SmlI C'TyrA_G
Cuts at: 1483 2431 2455 2878 3793 4285 4547 1483
Size: 948 24 423 915 492 262 2659
Fragments arranged by size:
2659 948 915 492 423 262 24
SnaBI TAC'GTA
Cuts at: 2715 2715
Size: 5723
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5723
SphI G_CATG'C
Cuts at: 2644 2776 3276 2644
Size: 132 500 5091
Fragments arranged by size:
5091 500 132
StuI AGG'CCT
Cuts at: 490 877 2302 490
Size: 387 1425 3911
Fragments arranged by size:
3911 1425 387
StyI C'CwwG_G
Cuts at: 125 446 660 797 1313 2043 2063 2519 2857
Size: 321 214 137 516 730 20 456 338
Cuts at: 2857 3211 125
Size: 354 2637
Fragments arranged by size:
2637 730 516 456 354 338 321 214
137 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1185 1320 2410 2440 3306 3439 3898 4107 1185
Size: 135 1090 30 866 133 459 209 2801
Fragments arranged by size:
2801 1090 866 459 209 135 133 30
TatI w'GTAC_w
Cuts at: 954 2024 5238 954
Size: 1070 3214 1439
Fragments arranged by size:
3214 1439 1070
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 228 260 5240 5272 228
Size: 32 4980 32 679
Fragments arranged by size:
4980 679 32 32
Tth111I GACn'n_nGTC
Cuts at: 3520 3520
Size: 5723
XbaI T'CTAG_A
Cuts at: 2754 2754
Size: 5723
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1090 1750 3218 1090
Size: 660 1468 3595
Fragments arranged by size:
3595 1468 660
XmaI C'CCGG_G
Cuts at: 2073 2743 2073
Size: 670 5053
XmnI GAAnn'nnTTC
Cuts at: 2278 2278
Size: 5723
ZraI GAC'GTC
Cuts at: 1659 1659
Size: 5723
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AscI BaeI BcgI BfrBI BglI BmgBI
BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
ClaI DraIII EcoRV FseI FspAI HpaI MfeI NheI
NotI NruI NsiI PacI PflMI PsiI PspXI SexAI
SfiI SgrAI SrfI SspI SwaI XhoI