Restriction map for
pF1KSDA1052
(Circular) (Six-base) MAPSORT of: pF1KSDA1052.seq Check: 6951 from: 1 to: 7504
pF1KSDA1052 7504 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1400 1740 1970 1400
Size: 340 230 6934
Fragments arranged by size:
6934 340 230
AatII G_ACGT'C
Cuts at: 657 657
Size: 7504
AccI GT'mk_AC
Cuts at: 4540 4540
Size: 7504
Acc65I G'GTAC_C
Cuts at: 1535 4518 1535
Size: 2983 4521
AclI AA'CG_TT
Cuts at: 7344 7344
Size: 7504
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1625 3058 3181 3204 3257 3712 5327 5759 6508
Size: 1433 123 23 53 455 1615 432 749
Cuts at: 6508 6776 1625
Size: 268 2353
Fragments arranged by size:
2353 1615 1433 749 455 432 268 123
53 23
AflIII A'CryG_T
Cuts at: 4262 4374 4854 5960 6937 4262
Size: 112 480 1106 977 4829
Fragments arranged by size:
4829 1106 977 480 112
AgeI A'CCGG_T
Cuts at: 5892 5892
Size: 7504
AhdI GACnn_n'nnGTC
Cuts at: 1341 1482 3414 1341
Size: 141 1932 5431
Fragments arranged by size:
5431 1932 141
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2656 4234 2656
Size: 1578 5926
AlwNI CAG_nnn'CTG
Cuts at: 949 1873 1903 1995 2229 2385 3009 3072 3207
Size: 924 30 92 234 156 624 63 135
Cuts at: 3207 3258 4807 6376 949
Size: 51 1549 1569 2077
Fragments arranged by size:
2077 1569 1549 924 624 234 156 135
92 63 51 30
ApaI G_GGCC'C
Cuts at: 1578 1627 1786 2817 4136 1578
Size: 49 159 1031 1319 4946
Fragments arranged by size:
4946 1319 1031 159 49
ApaLI G'TGCA_C
Cuts at: 6274 6274
Size: 7504
ApoI r'AATT_y
Cuts at: 209 217 3131 4506 6857 7113 209
Size: 8 2914 1375 2351 256 600
Fragments arranged by size:
2914 2351 1375 600 256 8
AseI AT'TA_AT
Cuts at: 20 20
Size: 7504
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7504
AvaI C'yCGr_G
Cuts at: 224 1627 1639 1942 3870 4019 4522 224
Size: 1403 12 303 1928 149 503 3206
Fragments arranged by size:
3206 1928 1403 503 303 149 12
BamHI G'GATC_C
Cuts at: 1806 3769 4527 1806
Size: 1963 758 4783
Fragments arranged by size:
4783 1963 758
BanI G'GyrC_C
Cuts at: 1535 1958 2780 2876 3434 3548 3644 4518 4932
Size: 423 822 96 558 114 96 874 414
Cuts at: 4932 5180 5215 1535
Size: 248 35 3824
Fragments arranged by size:
3824 874 822 558 423 414 248 114
96 96 35
BanII G_rGCy'C
Cuts at: 640 727 1578 1616 1627 1786 2160 2630 2817
Size: 87 851 38 11 159 374 470 187
Cuts at: 2817 4136 4516 5546 640
Size: 1319 380 1030 2598
Fragments arranged by size:
2598 1319 1030 851 470 380 374 187
159 87 38 11
BbeI G_GCGC'C
Cuts at: 2880 3552 4936 5184 2880
Size: 672 1384 248 5200
Fragments arranged by size:
5200 1384 672 248
BbsI GAAGACnn'nnnn_
Cuts at: 1045 1472 3362 1045
Size: 427 1890 5187
Fragments arranged by size:
5187 1890 427
BbvCI CC'TCA_GC
Cuts at: 1687 2386 2747 3449 3946 1687
Size: 699 361 702 497 5245
Fragments arranged by size:
5245 702 699 497 361
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2329 2363 2329
Size: 34 7470
BciVI GTATCCnnnnn_n'
Cuts at: 582 5400 6163 7504 582
Size: 4818 763 1341 582
Fragments arranged by size:
4818 1341 763 582
BclI T'GATC_A
Cuts at: 5022 5022
Size: 7504
BglI GCCn_nnn'nGGC
Cuts at: 2959 2959
Size: 7504
BglII A'GATC_T
Cuts at: 3312 5017 7056 3312
Size: 1705 2039 3760
Fragments arranged by size:
3760 2039 1705
BlpI GC'TnA_GC
Cuts at: 2204 2450 2629 2768 3901 3934 4222 4614 2204
Size: 246 179 139 1133 33 288 392 5094
Fragments arranged by size:
5094 1133 392 288 246 179 139 33
Bme1580I G_kGCm'C
Cuts at: 867 1578 1627 1786 2783 2817 3760 4136 5127
Size: 711 49 159 997 34 943 376 991
Cuts at: 5127 5220 6278 867
Size: 93 1058 2093
Fragments arranged by size:
2093 1058 997 991 943 711 376 159
93 49 34
BmgBI CAC'GTC
Cuts at: 4100 4100
Size: 7504
BmrI ACTGGGnnnn_n'
Cuts at: 535 877 1411 1465 4714 4795 5130 7276 535
Size: 342 534 54 3249 81 335 2146 763
Fragments arranged by size:
3249 2146 763 534 342 335 81 54
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1604 1911 2517 5869 1604
Size: 307 606 3352 3239
Fragments arranged by size:
3352 3239 606 307
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 963 1546 1690 1835 2320 2452 2476 2487
Size: 640 583 144 145 485 132 24 11
Cuts at: 2487 2494 2818 2851 2890 2989 3094 3187 3250
Size: 7 324 33 39 99 105 93 63
Cuts at: 3250 3649 4264 323
Size: 399 615 3563
Fragments arranged by size:
3563 640 615 583 485 399 324 145
144 132 105 99 93 63 39 33
24 11 7
Bpu10I CC'TnA_GC
Cuts at: 1687 2159 2386 2723 2747 3449 3820 3946 1687
Size: 472 227 337 24 702 371 126 5245
Fragments arranged by size:
5245 702 472 371 337 227 126 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 810 2094 2293 2884 4678 5557 6051 6349
Size: 134 1284 199 591 1794 879 494 298
Cuts at: 6349 676
Size: 1831
Fragments arranged by size:
1831 1794 1284 879 591 494 298 199
134
BsaI GGTCTCn'nnnn_
Cuts at: 178 491 694 721 2505 2977 3631 7208 178
Size: 313 203 27 1784 472 654 3577 474
Fragments arranged by size:
3577 1784 654 474 472 313 203 27
BsaAI yAC'GTr
Cuts at: 2607 4494 4855 2607
Size: 1887 361 5256
Fragments arranged by size:
5256 1887 361
BsaBI GATnn'nnATC
Cuts at: 6 5016 7055 6
Size: 5010 2039 455
Fragments arranged by size:
5010 2039 455
BsaHI Gr'CG_yC
Cuts at: 654 1268 2877 3549 4933 5181 5885 7381 654
Size: 614 1609 672 1384 248 704 1496 777
Fragments arranged by size:
1609 1496 1384 777 704 672 614 248
BsaWI w'CCGG_w
Cuts at: 2788 4358 4691 5212 5892 6166 6313 6724 2788
Size: 1570 333 521 680 274 147 411 3568
Fragments arranged by size:
3568 1570 680 521 411 333 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 125 155 1519 1549 1993 2023 4042 4072 125
Size: 30 1364 30 444 30 2019 30 3557
Fragments arranged by size:
3557 2019 1364 444 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 799 808 904 931 1207 1541 1543 1582 1672
Size: 9 96 27 276 334 2 39 90
Cuts at: 1672 1789 1828 2032 2041 2116 2119 2122 2140
Size: 117 39 204 9 75 3 3 18
Cuts at: 2140 2460 2557 2694 2716 2764 2995 3091 3430
Size: 320 97 137 22 48 231 96 339
Cuts at: 3430 3718 3979 4137 799
Size: 288 261 158 4166
Fragments arranged by size:
4166 339 334 320 288 276 261 231
204 158 137 117 97 96 96 90
75 48 39 39 27 22 18 9
9 3 3 2
BseYI C'CCAG_C
Cuts at: 1445 1779 2549 2611 3536 4007 4254 4449 4927
Size: 334 770 62 925 471 247 195 478
Cuts at: 4927 5685 6264 6760 1445
Size: 758 579 496 2189
Fragments arranged by size:
2189 925 770 758 579 496 478 471
334 247 195 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1010 1889 3040 3508 1010
Size: 879 1151 468 5006
Fragments arranged by size:
5006 1151 879 468
BsiEI CG_ry'CG
Cuts at: 104 5044 5090 6300 104
Size: 4940 46 1210 1308
Fragments arranged by size:
4940 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 640 1391 2160 2444 2582 2624 2630 2708 3095
Size: 751 769 284 138 42 6 78 387
Cuts at: 3095 4036 4466 4516 5294 5484 6278 640
Size: 941 430 50 778 190 794 1866
Fragments arranged by size:
1866 941 794 778 769 751 430 387
284 190 138 78 50 42 6
BsmI GAATG_Cn'
Cuts at: 1761 2361 1761
Size: 600 6904
Bsp1286I G_dGCh'C
Cuts at: 640 727 867 1391 1578 1616 1627 1786 2160
Size: 87 140 524 187 38 11 159 374
Cuts at: 2160 2444 2582 2624 2630 2708 2783 2817 3095
Size: 284 138 42 6 78 75 34 278
Cuts at: 3095 3760 4036 4136 4466 4516 5127 5220 5294
Size: 665 276 100 330 50 611 93 74
Cuts at: 5294 5484 5546 6278 640
Size: 190 62 732 1866
Fragments arranged by size:
1866 732 665 611 524 374 330 284
278 276 190 187 159 140 138 100
93 87 78 75 74 62 50 42
38 34 11 6
BspEI T'CCGG_A
Cuts at: 6724 6724
Size: 7504
BspHI T'CATG_A
Cuts at: 6680 6680
Size: 7504
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1400 1740 1970 3018 3695 4552 4766 5068 5449
Size: 340 230 1048 677 857 214 302 381
Cuts at: 5449 1400
Size: 3455
Fragments arranged by size:
3455 1048 857 677 381 340 302 230
214
BsrBI CCG'CTC
Cuts at: 50 423 1572 2082 2460 3987 5794 5848 7334
Size: 373 1149 510 378 1527 1807 54 1486
Cuts at: 7334 7499 50
Size: 165 55
Fragments arranged by size:
1807 1527 1486 1149 510 378 373 165
55 54
BsrDI GCAATG_nn'
Cuts at: 5414 5414
Size: 7504
BsrFI r'CCGG_y
Cuts at: 952 2879 5500 5681 5892 952
Size: 1927 2621 181 211 2564
Fragments arranged by size:
2621 2564 1927 211 181
BssSI C'ACGA_G
Cuts at: 1564 2623 5773 6133 1564
Size: 1059 3150 360 2935
Fragments arranged by size:
3150 2935 1059 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1995 3009 4879 1995
Size: 1014 1870 4620
Fragments arranged by size:
4620 1870 1014
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7504
BstEII G'GTnAC_C
Cuts at: 1107 4394 1107
Size: 3287 4217
BstXI CCAn_nnnn'nTGG
Cuts at: 1524 3648 4242 1524
Size: 2124 594 4786
Fragments arranged by size:
4786 2124 594
BstYI r'GATC_y
Cuts at: 1 613 1379 1806 2030 3180 3312 3769 3890
Size: 612 766 427 224 1150 132 457 121
Cuts at: 3890 4527 4994 5017 5352 5598 6612 6698 6710
Size: 637 467 23 335 246 1014 86 12
Cuts at: 6710 7056 1
Size: 346 449
Fragments arranged by size:
1150 1014 766 637 612 467 457 449
427 346 335 246 224 132 121 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1811 3331 4316 1811
Size: 1520 985 4999
Fragments arranged by size:
4999 1520 985
BtgI C'CryG_G
Cuts at: 125 1350 1916 3894 125
Size: 1225 566 1978 3735
Fragments arranged by size:
3735 1978 1225 566
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5452 5633 5796 5452
Size: 181 163 7160
Fragments arranged by size:
7160 181 163
BtsI GCAGTG_nn'
Cuts at: 295 1014 1140 1934 3831 3945 4745 295
Size: 719 126 794 1897 114 800 3054
Fragments arranged by size:
3054 1897 800 794 719 126 114
DraI TTT'AAA
Cuts at: 4501 4501
Size: 7504
DrdI GACnn_nn'nnGTC
Cuts at: 5208 6068 5208
Size: 860 6644
EaeI y'GGCC_r
Cuts at: 1598 3560 3578 3665 4064 5087 5261 5652 5679
Size: 1962 18 87 399 1023 174 391 27
Cuts at: 5679 7026 1598
Size: 1347 2076
Fragments arranged by size:
2076 1962 1347 1023 399 391 174 87
27 18
EagI C'GGCC_G
Cuts at: 5087 5087
Size: 7504
EarI CTCTTCn'nnn_
Cuts at: 611 1072 1205 1247 1604 1790 1916 2018 2231
Size: 461 133 42 357 186 126 102 213
Cuts at: 2231 2309 2329 3034 3734 4408 4413 5525 5735
Size: 78 20 705 700 674 5 1112 210
Cuts at: 5735 611
Size: 2380
Fragments arranged by size:
2380 1112 705 700 674 461 357 213
210 186 133 126 102 78 42 20
5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5710 6022 6168 7314 5710
Size: 312 146 1146 5900
Fragments arranged by size:
5900 1146 312 146
EcoICRI GAG'CTC
Cuts at: 638 2158 2628 4514 638
Size: 1520 470 1886 3628
Fragments arranged by size:
3628 1886 1520 470
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 963 1546 1625 1690 1835 2320 2452 2476
Size: 640 583 79 65 145 485 132 24
Cuts at: 2476 2487 2494 2818 2851 2890 2989 3058 3094
Size: 11 7 324 33 39 99 69 36
Cuts at: 3094 3181 3187 3204 3250 3257 3649 3712 4264
Size: 87 6 17 46 7 392 63 552
Cuts at: 4264 5327 5759 6508 6776 323
Size: 1063 432 749 268 1051
Fragments arranged by size:
1063 1051 749 640 583 552 485 432
392 324 268 145 132 99 87 79
69 65 63 46 39 36 33 24
17 11 7 7 6
EcoNI CCTnn'n_nnAGG
Cuts at: 575 3631 575
Size: 3056 4448
EcoO109I rG'GnC_Cy
Cuts at: 561 1623 1624 1719 1783 2813 3327 3603 4023
Size: 1062 1 95 64 1030 514 276 420
Cuts at: 4023 4132 4641 561
Size: 109 509 3424
Fragments arranged by size:
3424 1062 1030 514 509 420 276 109
95 64 1
EcoRI G'AATT_C
Cuts at: 4506 4506
Size: 7504
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 229 735 3414 5521 229
Size: 506 2679 2107 2212
Fragments arranged by size:
2679 2212 2107 506
FspI TGC'GCA
Cuts at: 3569 3619 5283 3569
Size: 50 1664 5790
Fragments arranged by size:
5790 1664 50
HaeII r_GCGC'y
Cuts at: 2655 2805 2880 3552 4936 5184 6208 7187 2655
Size: 150 75 672 1384 248 1024 979 2972
Fragments arranged by size:
2972 1384 1024 979 672 248 150 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 125 157 269 301 1327 1359 1468 1500 1846
Size: 32 112 32 1026 32 109 32 346
Cuts at: 1846 1878 2147 2179 2230 2262 3400 3432 3646
Size: 32 269 32 51 32 1138 32 214
Cuts at: 3646 3678 5344 5374 5376 5406 125
Size: 32 1666 30 2 30 2223
Fragments arranged by size:
2223 1666 1138 1026 346 269 214 112
109 51 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 437 4541 437
Size: 4104 3400
KasI G'GCGC_C
Cuts at: 2876 3548 4932 5180 2876
Size: 672 1384 248 5200
Fragments arranged by size:
5200 1384 672 248
KpnI G_GTAC'C
Cuts at: 1539 4522 1539
Size: 2983 4521
MluI A'CGCG_T
Cuts at: 6937 6937
Size: 7504
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2222 2666 2900 3701 4160 6175 6359 2222
Size: 444 234 801 459 2015 184 3367
Fragments arranged by size:
3367 2015 801 459 444 234 184
MscI TGG'CCA
Cuts at: 1600 3562 3580 4066 5263 1600
Size: 1962 18 486 1197 3841
Fragments arranged by size:
3841 1962 1197 486 18
MslI CAynn'nnrTG
Cuts at: 386 3650 5491 5618 386
Size: 3264 1841 127 2272
Fragments arranged by size:
3264 2272 1841 127
MspA1I CmG'CkG
Cuts at: 159 1698 2385 2397 2553 2763 3009 3072 3933
Size: 1539 687 12 156 210 246 63 861
Cuts at: 3933 4218 4242 4449 4613 4927 5287 6302 6547
Size: 285 24 207 164 314 360 1015 245
Cuts at: 6547 159
Size: 1116
Fragments arranged by size:
1539 1116 1015 861 687 360 314 285
246 245 210 207 164 156 63 24
12
NaeI GCC'GGC
Cuts at: 2881 5683 2881
Size: 2802 4702
NarI GG'CG_CC
Cuts at: 2877 3549 4933 5181 2877
Size: 672 1384 248 5200
Fragments arranged by size:
5200 1384 672 248
NcoI C'CATG_G
Cuts at: 125 1350 1916 125
Size: 1225 566 5713
Fragments arranged by size:
5713 1225 566
NgoMIV G'CCGG_C
Cuts at: 2879 5681 2879
Size: 2802 4702
NruI TCG'CGA
Cuts at: 5865 5865
Size: 7504
NspI r_CATG'y
Cuts at: 4266 4555 5055 5964 4266
Size: 289 500 909 5806
Fragments arranged by size:
5806 909 500 289
PasI CC'CwG_GG
Cuts at: 1620 1786 1620
Size: 166 7338
PciI A'CATG_T
Cuts at: 4262 5960 4262
Size: 1698 5806
PflMI CCAn_nnn'nTGG
Cuts at: 301 1281 1356 2661 3558 4109 301
Size: 980 75 1305 897 551 3696
Fragments arranged by size:
3696 1305 980 897 551 75
PfoI T'CCnGG_A
Cuts at: 1201 2906 2975 3953 1201
Size: 1705 69 978 4752
Fragments arranged by size:
4752 1705 978 69
PmeI GTTT'AAAC
Cuts at: 4501 4501
Size: 7504
PmlI CAC'GTG
Cuts at: 2607 2607
Size: 7504
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1298 1330 6643 6675 1298
Size: 32 5313 32 2127
Fragments arranged by size:
5313 2127 32 32
PpuMI rG'GwC_Cy
Cuts at: 3327 4023 3327
Size: 696 6808
PshAI GACnn'nnGTC
Cuts at: 998 998
Size: 7504
PspOMI G'GGCC_C
Cuts at: 1574 1623 1782 2813 4132 1574
Size: 49 159 1031 1319 4946
Fragments arranged by size:
4946 1319 1031 159 49
PspXI vC'TCGA_Gb
Cuts at: 1942 1942
Size: 7504
PstI C_TGCA'G
Cuts at: 1505 1680 1697 2090 2225 2381 2558 2810 3149
Size: 175 17 393 135 156 177 252 339
Cuts at: 3149 3191 3212 4448 4549 5234 1505
Size: 42 21 1236 101 685 3775
Fragments arranged by size:
3775 1236 685 393 339 252 177 175
156 135 101 42 21 17
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7504
PvuII CAG'CTG
Cuts at: 159 1698 2385 2397 2553 3009 3072 3933 4449
Size: 1539 687 12 156 456 63 861 516
Cuts at: 4449 4927 5287 159
Size: 478 360 2376
Fragments arranged by size:
2376 1539 861 687 516 478 456 360
156 63 12
RsrII CG'GwC_CG
Cuts at: 5697 5697
Size: 7504
SacI G_AGCT'C
Cuts at: 640 2160 2630 4516 640
Size: 1520 470 1886 3628
Fragments arranged by size:
3628 1886 1520 470
SalI G'TCGA_C
Cuts at: 4539 4539
Size: 7504
SanDI GG'GwC_CC
Cuts at: 4023 4023
Size: 7504
SapI GCTCTTCn'nnn_
Cuts at: 611 1604 2329 5525 5735 611
Size: 993 725 3196 210 2380
Fragments arranged by size:
3196 2380 993 725 210
SbfI CC_TGCA'GG
Cuts at: 4549 4549
Size: 7504
ScaI AGT'ACT
Cuts at: 1940 7021 1940
Size: 5081 2423
SexAI A'CCwGG_T
Cuts at: 1047 1047
Size: 7504
SfcI C'TryA_G
Cuts at: 33 1127 1501 1676 1693 2086 2221 2377 2554
Size: 1094 374 175 17 393 135 156 177
Cuts at: 2554 2806 3145 3187 3208 4291 4444 4545 5230
Size: 252 339 42 21 1083 153 101 685
Cuts at: 5230 6225 6416 33
Size: 995 191 1121
Fragments arranged by size:
1121 1094 1083 995 685 393 374 339
252 191 177 175 156 153 135 101
42 21 17
SfoI GGC'GCC
Cuts at: 2878 3550 4934 5182 2878
Size: 672 1384 248 5200
Fragments arranged by size:
5200 1384 672 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7504
SmaI CCC'GGG
Cuts at: 226 4021 4524 226
Size: 3795 503 3206
Fragments arranged by size:
3795 3206 503
SmlI C'TyrA_G
Cuts at: 691 825 1942 2073 2308 2863 4657 5572 6066
Size: 134 1117 131 235 555 1794 915 494
Cuts at: 6066 6328 691
Size: 262 1867
Fragments arranged by size:
1867 1794 1117 915 555 494 262 235
134 131
SnaBI TAC'GTA
Cuts at: 4494 4494
Size: 7504
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7504
SphI G_CATG'C
Cuts at: 4555 5055 4555
Size: 500 7004
StuI AGG'CCT
Cuts at: 1523 3745 1523
Size: 2222 5282
StyI C'CwwG_G
Cuts at: 125 521 1115 1220 1350 1916 2565 3342 3473
Size: 396 594 105 130 566 649 777 131
Cuts at: 3473 4636 4990 125
Size: 1163 354 2639
Fragments arranged by size:
2639 1163 777 649 594 566 396 354
131 130 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1414 3076 3112 4110 4120 4431 5085 5218 5677
Size: 1662 36 998 10 311 654 133 459
Cuts at: 5677 5888 1414
Size: 211 3030
Fragments arranged by size:
3030 1662 998 654 459 311 211 133
36 10
TatI w'GTAC_w
Cuts at: 1938 7019 1938
Size: 5081 2423
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1993 2025 7021 7053 1993
Size: 32 4996 32 2444
Fragments arranged by size:
4996 2444 32 32
Tth111I GACn'n_nGTC
Cuts at: 1049 5299 1049
Size: 4250 3254
XbaI T'CTAG_A
Cuts at: 4533 4533
Size: 7504
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 263 513 1278 1872 4997 263
Size: 250 765 594 3125 2770
Fragments arranged by size:
3125 2770 765 594 250
XhoI C'TCGA_G
Cuts at: 1942 1942
Size: 7504
XmaI C'CCGG_G
Cuts at: 224 4019 4522 224
Size: 3795 503 3206
Fragments arranged by size:
3795 3206 503
XmnI GAAnn'nnTTC
Cuts at: 3217 3217
Size: 7504
ZraI GAC'GTC
Cuts at: 655 655
Size: 7504
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AleI AloI AscI AvrII BaeI BfrBI
BmtI BsiWI BsmBI BsrGI BssHII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NsiI PacI PsiI PsrI SacII SfiI
SgrAI SrfI SspI SwaI