Restriction map for
pF1KSDA1045
(Circular) (Six-base) MAPSORT of: pF1KSDA1045.seq Check: 2499 from: 1 to: 4339
pF1KSDA1045 4339 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 153 153
Size: 4339
AccI GT'mk_AC
Cuts at: 1375 1375
Size: 4339
Acc65I G'GTAC_C
Cuts at: 1353 1353
Size: 4339
AclI AA'CG_TT
Cuts at: 4179 4179
Size: 4339
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1333 2162 2594 3343 3611 1333
Size: 829 432 749 268 2061
Fragments arranged by size:
2061 829 749 432 268
AflIII A'CryG_T
Cuts at: 1689 2795 3772 1689
Size: 1106 977 2256
Fragments arranged by size:
2256 1106 977
AgeI A'CCGG_T
Cuts at: 2727 2727
Size: 4339
AhdI GACnn_n'nnGTC
Cuts at: 930 930
Size: 4339
AleI CACnn'nnGTG
Cuts at: 686 686
Size: 4339
AlwNI CAG_nnn'CTG
Cuts at: 572 1642 3211 572
Size: 1070 1569 1700
Fragments arranged by size:
1700 1569 1070
ApaI G_GGCC'C
Cuts at: 843 1083 843
Size: 240 4099
ApaLI G'TGCA_C
Cuts at: 3109 3109
Size: 4339
ApoI r'AATT_y
Cuts at: 1341 3692 3948 1341
Size: 2351 256 1732
Fragments arranged by size:
2351 1732 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4339
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4339
AvaI C'yCGr_G
Cuts at: 1019 1037 1357 1019
Size: 18 320 4001
Fragments arranged by size:
4001 320 18
BamHI G'GATC_C
Cuts at: 1362 1362
Size: 4339
BanI G'GyrC_C
Cuts at: 1353 1767 2015 2050 1353
Size: 414 248 35 3642
Fragments arranged by size:
3642 414 248 35
BanII G_rGCy'C
Cuts at: 277 494 843 1020 1083 1351 2381 277
Size: 217 349 177 63 268 1030 2235
Fragments arranged by size:
2235 1030 349 268 217 177 63
BbeI G_GCGC'C
Cuts at: 1771 2019 1771
Size: 248 4091
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 302 336 302
Size: 34 4305
BciVI GTATCCnnnnn_n'
Cuts at: 1318 2235 2998 4339 1318
Size: 917 763 1341 1318
Fragments arranged by size:
1341 1318 917 763
BclI T'GATC_A
Cuts at: 1857 1857
Size: 4339
BglI GCCn_nnn'nGGC
Cuts at: 611 1033 611
Size: 422 3917
BglII A'GATC_T
Cuts at: 1852 3891 1852
Size: 2039 2300
BlpI GC'TnA_GC
Cuts at: 1449 1449
Size: 4339
Bme1580I G_kGCm'C
Cuts at: 843 1083 1962 2055 3113 843
Size: 240 879 93 1058 2069
Fragments arranged by size:
2069 1058 879 240 93
BmrI ACTGGGnnnn_n'
Cuts at: 1549 1630 1965 4111 1549
Size: 81 335 2146 1777
Fragments arranged by size:
2146 1777 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2704 2704
Size: 4339
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 508 693 805 458
Size: 50 185 112 3992
Fragments arranged by size:
3992 185 112 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1513 2392 2886 3184 1513
Size: 879 494 298 2668
Fragments arranged by size:
2668 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 733 4043 733
Size: 3310 1029
BsaAI yAC'GTr
Cuts at: 1329 1690 1329
Size: 361 3978
BsaBI GATnn'nnATC
Cuts at: 6 1851 3890 6
Size: 1845 2039 455
Fragments arranged by size:
2039 1845 455
BsaHI Gr'CG_yC
Cuts at: 1768 2016 2720 4216 1768
Size: 248 704 1496 1891
Fragments arranged by size:
1891 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1526 2047 2727 3001 3148 3559 1526
Size: 521 680 274 147 411 2306
Fragments arranged by size:
2306 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 709 739 709
Size: 30 4309
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 310 412 730 865 310
Size: 102 318 135 3784
Fragments arranged by size:
3784 318 135 102
BseYI C'CCAG_C
Cuts at: 1082 1170 1762 2520 3099 3595 1082
Size: 88 592 758 579 496 1826
Fragments arranged by size:
1826 758 592 579 496 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 558 585 653 187
Size: 371 27 68 3873
Fragments arranged by size:
3873 371 68 27
BsiEI CG_ry'CG
Cuts at: 104 351 1879 1925 3135 104
Size: 247 1528 46 1210 1308
Fragments arranged by size:
1528 1308 1210 247 46
BsiHKAI G_wGCw'C
Cuts at: 1054 1351 2129 2319 3113 1054
Size: 297 778 190 794 2280
Fragments arranged by size:
2280 794 778 297 190
Bsp1286I G_dGCh'C
Cuts at: 277 494 843 1020 1054 1083 1351 1962 2055
Size: 217 349 177 34 29 268 611 93
Cuts at: 2055 2129 2319 2381 3113 277
Size: 74 190 62 732 1503
Fragments arranged by size:
1503 732 611 349 268 217 190 177
93 74 62 34 29
BspEI T'CCGG_A
Cuts at: 3559 3559
Size: 4339
BspHI T'CATG_A
Cuts at: 3515 3515
Size: 4339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 153 1387 1601 1903 2284 153
Size: 1234 214 302 381 2208
Fragments arranged by size:
2208 1234 381 302 214
BsrBI CCG'CTC
Cuts at: 50 366 1008 2629 2683 4169 4334 50
Size: 316 642 1621 54 1486 165 55
Fragments arranged by size:
1621 1486 642 316 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2249 2249
Size: 4339
BsrFI r'CCGG_y
Cuts at: 346 2335 2516 2727 346
Size: 1989 181 211 1958
Fragments arranged by size:
1989 1958 211 181
BssSI C'ACGA_G
Cuts at: 2608 2968 2608
Size: 360 3979
BstAPI GCAn_nnn'nTGC
Cuts at: 572 963 1714 572
Size: 391 751 3197
Fragments arranged by size:
3197 751 391
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4339
BstXI CCAn_nnnn'nTGG
Cuts at: 1236 1236
Size: 4339
BstYI r'GATC_y
Cuts at: 1 1362 1829 1852 2187 2433 3447 3533 3545
Size: 1361 467 23 335 246 1014 86 12
Cuts at: 3545 3891 1
Size: 346 449
Fragments arranged by size:
1361 1014 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 395 395
Size: 4339
BtgI C'CryG_G
Cuts at: 125 258 125
Size: 133 4206
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1140 2287 2468 2631 1140
Size: 1147 181 163 2848
Fragments arranged by size:
2848 1147 181 163
BtsI GCAGTG_nn'
Cuts at: 1050 1134 1580 1050
Size: 84 446 3809
Fragments arranged by size:
3809 446 84
DraI TTT'AAA
Cuts at: 1336 1336
Size: 4339
DrdI GACnn_nn'nnGTC
Cuts at: 2043 2903 2043
Size: 860 3479
EaeI y'GGCC_r
Cuts at: 348 1922 2096 2487 2514 3861 348
Size: 1574 174 391 27 1347 826
Fragments arranged by size:
1574 1347 826 391 174 27
EagI C'GGCC_G
Cuts at: 348 1922 348
Size: 1574 2765
EarI CTCTTCn'nnn_
Cuts at: 329 562 854 2360 2570 329
Size: 233 292 1506 210 2098
Fragments arranged by size:
2098 1506 292 233 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 430 2545 2857 3003 4149 430
Size: 2115 312 146 1146 620
Fragments arranged by size:
2115 1146 620 312 146
EcoICRI GAG'CTC
Cuts at: 1349 1349
Size: 4339
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 508 693 805 1333 2162 2594 3343 3611
Size: 50 185 112 528 829 432 749 268
Cuts at: 3611 458
Size: 1186
Fragments arranged by size:
1186 829 749 528 432 268 185 112
50
EcoNI CCTnn'n_nnAGG
Cuts at: 847 847
Size: 4339
EcoO109I rG'GnC_Cy
Cuts at: 251 839 840 1150 1476 251
Size: 588 1 310 326 3114
Fragments arranged by size:
3114 588 326 310 1
EcoRI G'AATT_C
Cuts at: 1341 1341
Size: 4339
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2356 2356
Size: 4339
FspI TGC'GCA
Cuts at: 2118 2118
Size: 4339
HaeII r_GCGC'y
Cuts at: 1771 2019 3043 4022 1771
Size: 248 1024 979 2088
Fragments arranged by size:
2088 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 916 948 1210 1242 2179 2209 2211 2241 916
Size: 32 262 32 937 30 2 30 3014
Fragments arranged by size:
3014 937 262 32 32 30 30 2
HincII GTy'rAC
Cuts at: 510 1376 510
Size: 866 3473
KasI G'GCGC_C
Cuts at: 1767 2015 1767
Size: 248 4091
KpnI G_GTAC'C
Cuts at: 1357 1357
Size: 4339
MluI A'CGCG_T
Cuts at: 3772 3772
Size: 4339
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 252 3010 3194 252
Size: 2758 184 1397
Fragments arranged by size:
2758 1397 184
MscI TGG'CCA
Cuts at: 2098 2098
Size: 4339
MslI CAynn'nnrTG
Cuts at: 130 686 2326 2453 130
Size: 556 1640 127 2016
Fragments arranged by size:
2016 1640 556 127
MspA1I CmG'CkG
Cuts at: 547 637 750 1448 1762 2122 3137 3382 547
Size: 90 113 698 314 360 1015 245 1504
Fragments arranged by size:
1504 1015 698 360 314 245 113 90
NaeI GCC'GGC
Cuts at: 348 2518 348
Size: 2170 2169
NarI GG'CG_CC
Cuts at: 1768 2016 1768
Size: 248 4091
NcoI C'CATG_G
Cuts at: 125 125
Size: 4339
NgoMIV G'CCGG_C
Cuts at: 346 2516 346
Size: 2170 2169
NruI TCG'CGA
Cuts at: 2700 2700
Size: 4339
NspI r_CATG'y
Cuts at: 1390 1890 2799 1390
Size: 500 909 2930
Fragments arranged by size:
2930 909 500
PciI A'CATG_T
Cuts at: 2795 2795
Size: 4339
PfoI T'CCnGG_A
Cuts at: 281 329 281
Size: 48 4291
PmeI GTTT'AAAC
Cuts at: 1336 1336
Size: 4339
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3478 3510 3478
Size: 32 4307
PpuMI rG'GwC_Cy
Cuts at: 251 1150 251
Size: 899 3440
PspOMI G'GGCC_C
Cuts at: 839 1079 839
Size: 240 4099
PstI C_TGCA'G
Cuts at: 959 1129 1384 2069 959
Size: 170 255 685 3229
Fragments arranged by size:
3229 685 255 170
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4339
PvuII CAG'CTG
Cuts at: 547 1762 2122 547
Size: 1215 360 2764
Fragments arranged by size:
2764 1215 360
RsrII CG'GwC_CG
Cuts at: 2532 2532
Size: 4339
SacI G_AGCT'C
Cuts at: 1351 1351
Size: 4339
SalI G'TCGA_C
Cuts at: 1374 1374
Size: 4339
SanDI GG'GwC_CC
Cuts at: 251 1150 251
Size: 899 3440
SapI GCTCTTCn'nnn_
Cuts at: 854 2360 2570 854
Size: 1506 210 2623
Fragments arranged by size:
2623 1506 210
SbfI CC_TGCA'GG
Cuts at: 1384 1384
Size: 4339
ScaI AGT'ACT
Cuts at: 3856 3856
Size: 4339
SfcI C'TryA_G
Cuts at: 33 266 955 1125 1380 2065 3060 3251 33
Size: 233 689 170 255 685 995 191 1121
Fragments arranged by size:
1121 995 689 685 255 233 191 170
SfoI GGC'GCC
Cuts at: 1769 2017 1769
Size: 248 4091
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4339
SmaI CCC'GGG
Cuts at: 1359 1359
Size: 4339
SmlI C'TyrA_G
Cuts at: 1492 2407 2901 3163 1492
Size: 915 494 262 2668
Fragments arranged by size:
2668 915 494 262
SnaBI TAC'GTA
Cuts at: 1329 1329
Size: 4339
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4339
SphI G_CATG'C
Cuts at: 1390 1890 1390
Size: 500 3839
StuI AGG'CCT
Cuts at: 219 219
Size: 4339
StyI C'CwwG_G
Cuts at: 125 621 1471 1825 125
Size: 496 850 354 2639
Fragments arranged by size:
2639 850 496 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1920 2053 2512 2723 1920
Size: 133 459 211 3536
Fragments arranged by size:
3536 459 211 133
TatI w'GTAC_w
Cuts at: 3854 3854
Size: 4339
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 308 340 3856 3888 308
Size: 32 3516 32 759
Fragments arranged by size:
3516 759 32 32
Tth111I GACn'n_nGTC
Cuts at: 2134 2134
Size: 4339
XbaI T'CTAG_A
Cuts at: 1368 1368
Size: 4339
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 453 1832 453
Size: 1379 2960
XmaI C'CCGG_G
Cuts at: 1357 1357
Size: 4339
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PciI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BaeI
BbsI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SrfI SspI
SwaI XhoI XmnI ZraI