Restriction map for
pF1KSDA1044
(Circular) (Six-base) MAPSORT of: pF1KSDA1044.seq Check: 6728 from: 1 to: 5027
pF1KSDA1044 5027 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1574 1717 1574
Size: 143 4884
AatII G_ACGT'C
Cuts at: 980 980
Size: 5027
AccI GT'mk_AC
Cuts at: 1213 1782 2065 1213
Size: 569 283 4175
Fragments arranged by size:
4175 569 283
Acc65I G'GTAC_C
Cuts at: 2043 2043
Size: 5027
AclI AA'CG_TT
Cuts at: 306 4867 306
Size: 4561 466
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1002 1096 1335 2852 3284 4031 4299 1002
Size: 94 239 1517 432 747 268 1730
Fragments arranged by size:
1730 1517 747 432 268 239 94
AflIII A'CryG_T
Cuts at: 2379 3483 4460 2379
Size: 1104 977 2946
Fragments arranged by size:
2946 1104 977
AgeI A'CCGG_T
Cuts at: 3415 3415
Size: 5027
AlwNI CAG_nnn'CTG
Cuts at: 1709 2332 3899 1709
Size: 623 1567 2837
Fragments arranged by size:
2837 1567 623
ApaLI G'TGCA_C
Cuts at: 3797 3797
Size: 5027
ApoI r'AATT_y
Cuts at: 414 2031 4380 4636 414
Size: 1617 2349 256 805
Fragments arranged by size:
2349 1617 805 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5027
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5027
AvaI C'yCGr_G
Cuts at: 195 216 2047 195
Size: 21 1831 3175
Fragments arranged by size:
3175 1831 21
BamHI G'GATC_C
Cuts at: 1421 2052 1421
Size: 631 4396
BanI G'GyrC_C
Cuts at: 271 1254 2043 2457 2705 2740 271
Size: 983 789 414 248 35 2558
Fragments arranged by size:
2558 983 789 414 248 35
BanII G_rGCy'C
Cuts at: 2041 3071 2041
Size: 1030 3997
BbeI G_GCGC'C
Cuts at: 2461 2709 2461
Size: 248 4779
BbsI GAAGACnn'nnnn_
Cuts at: 999 1008 1163 1676 999
Size: 9 155 513 4350
Fragments arranged by size:
4350 513 155 9
BciVI GTATCCnnnnn_n'
Cuts at: 576 1353 2925 3686 5027 576
Size: 777 1572 761 1341 576
Fragments arranged by size:
1572 1341 777 761 576
BclI T'GATC_A
Cuts at: 2547 2547
Size: 5027
BglII A'GATC_T
Cuts at: 2542 4579 2542
Size: 2037 2990
BlpI GC'TnA_GC
Cuts at: 2139 2139
Size: 5027
Bme1580I G_kGCm'C
Cuts at: 1398 2652 2745 3801 1398
Size: 1254 93 1056 2624
Fragments arranged by size:
2624 1254 1056 93
BmgBI CAC'GTC
Cuts at: 287 924 287
Size: 637 4390
BmrI ACTGGGnnnn_n'
Cuts at: 334 437 2239 2320 2655 4799 334
Size: 103 1802 81 335 2144 562
Fragments arranged by size:
2144 1802 562 335 103 81
BmtI G_CTAG'C
Cuts at: 1181 1181
Size: 5027
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 477 1844 3394 477
Size: 1367 1550 2110
Fragments arranged by size:
2110 1550 1367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 660 1524 1919 660
Size: 864 395 3768
Fragments arranged by size:
3768 864 395
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1031 2203 3082 3574 3872 1031
Size: 1172 879 492 298 2186
Fragments arranged by size:
2186 1172 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4731 4731
Size: 5027
BsaAI yAC'GTr
Cuts at: 2019 2380 2019
Size: 361 4666
BsaBI GATnn'nnATC
Cuts at: 6 1804 2541 4578 6
Size: 1798 737 2037 455
Fragments arranged by size:
2037 1798 737 455
BsaHI Gr'CG_yC
Cuts at: 977 2458 2706 3408 4904 977
Size: 1481 248 702 1496 1100
Fragments arranged by size:
1496 1481 1100 702 248
BsaWI w'CCGG_w
Cuts at: 1332 2216 2737 3415 3689 3836 4247 1332
Size: 884 521 678 274 147 411 2112
Fragments arranged by size:
2112 884 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 977 1007 977
Size: 30 4997
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 544 544
Size: 5027
BseYI C'CCAG_C
Cuts at: 595 1553 2452 3210 3787 4283 595
Size: 958 899 758 577 496 1339
Fragments arranged by size:
1339 958 899 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2569 2615 3823 104
Size: 2465 46 1208 1308
Fragments arranged by size:
2465 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2041 2819 3009 3801 2041
Size: 778 190 792 3267
Fragments arranged by size:
3267 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 720 1949 720
Size: 1229 3798
Bsp1286I G_dGCh'C
Cuts at: 1398 2041 2652 2745 2819 3009 3071 3801 1398
Size: 643 611 93 74 190 62 730 2624
Fragments arranged by size:
2624 730 643 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4247 4247
Size: 5027
BspHI T'CATG_A
Cuts at: 836 908 4203 836
Size: 72 3295 1660
Fragments arranged by size:
3295 1660 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1574 1717 2077 2291 2593 2974 1574
Size: 143 360 214 302 381 3627
Fragments arranged by size:
3627 381 360 302 214 143
BsrBI CCG'CTC
Cuts at: 50 795 3319 3373 4857 5022 50
Size: 745 2524 54 1484 165 55
Fragments arranged by size:
2524 1484 745 165 55 54
BsrDI GCAATG_nn'
Cuts at: 707 1316 2939 707
Size: 609 1623 2795
Fragments arranged by size:
2795 1623 609
BsrFI r'CCGG_y
Cuts at: 207 3025 3206 3415 207
Size: 2818 181 209 1819
Fragments arranged by size:
2818 1819 209 181
BssSI C'ACGA_G
Cuts at: 451 1597 3298 3656 451
Size: 1146 1701 358 1822
Fragments arranged by size:
1822 1701 1146 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2404 2404
Size: 5027
BstBI TT'CG_AA
Cuts at: 110 3388 110
Size: 3278 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 672 672
Size: 5027
BstYI r'GATC_y
Cuts at: 1 1022 1421 2052 2519 2542 2877 3123 4135
Size: 1021 399 631 467 23 335 246 1012
Cuts at: 4135 4221 4233 4579 1
Size: 86 12 346 449
Fragments arranged by size:
1021 1012 631 467 449 399 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1214 1783 1214
Size: 569 4458
BtgI C'CryG_G
Cuts at: 125 1121 125
Size: 996 4031
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 708 2977 3158 3321 708
Size: 2269 181 163 2414
Fragments arranged by size:
2414 2269 181 163
BtsI GCAGTG_nn'
Cuts at: 1572 2270 1572
Size: 698 4329
DraI TTT'AAA
Cuts at: 1853 2026 1853
Size: 173 4854
DrdI GACnn_nn'nnGTC
Cuts at: 1615 2733 3591 1615
Size: 1118 858 3051
Fragments arranged by size:
3051 1118 858
EaeI y'GGCC_r
Cuts at: 929 1415 2612 2786 3177 3204 4549 929
Size: 486 1197 174 391 27 1345 1407
Fragments arranged by size:
1407 1345 1197 486 391 174 27
EagI C'GGCC_G
Cuts at: 2612 2612
Size: 5027
EarI CTCTTCn'nnn_
Cuts at: 1072 1862 3050 3260 1072
Size: 790 1188 210 2839
Fragments arranged by size:
2839 1188 790 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 391 3235 3545 3691 4837 391
Size: 2844 310 146 1146 581
Fragments arranged by size:
2844 1146 581 310 146
EcoICRI GAG'CTC
Cuts at: 2039 2039
Size: 5027
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 660 1002 1096 1335 1524 1919 2852 3284 4031
Size: 342 94 239 189 395 933 432 747
Cuts at: 4031 4299 660
Size: 268 1388
Fragments arranged by size:
1388 933 747 432 395 342 268 239
189 94
EcoO109I rG'GnC_Cy
Cuts at: 199 236 644 2166 199
Size: 37 408 1522 3060
Fragments arranged by size:
3060 1522 408 37
EcoRI G'AATT_C
Cuts at: 2031 2031
Size: 5027
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3046 3046
Size: 5027
FspI TGC'GCA
Cuts at: 1057 2808 1057
Size: 1751 3276
HaeII r_GCGC'y
Cuts at: 567 608 880 2461 2709 3731 4710 567
Size: 41 272 1581 248 1022 979 884
Fragments arranged by size:
1581 1022 979 884 272 248 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 977 1009 1153 1185 2869 2899 2901 2931 977
Size: 32 144 32 1684 30 2 30 3073
Fragments arranged by size:
3073 1684 144 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2066 2066
Size: 5027
KasI G'GCGC_C
Cuts at: 2457 2705 2457
Size: 248 4779
KpnI G_GTAC'C
Cuts at: 2047 2047
Size: 5027
MluI A'CGCG_T
Cuts at: 4460 4460
Size: 5027
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1370 3698 3882 1370
Size: 2328 184 2515
Fragments arranged by size:
2515 2328 184
MscI TGG'CCA
Cuts at: 931 1417 2788 931
Size: 486 1371 3170
Fragments arranged by size:
3170 1371 486
MslI CAynn'nnrTG
Cuts at: 922 3016 3143 922
Size: 2094 127 2806
Fragments arranged by size:
2806 2094 127
MspA1I CmG'CkG
Cuts at: 169 595 983 1494 2138 2452 2812 3825 4070
Size: 426 388 511 644 314 360 1013 245
Cuts at: 4070 169
Size: 1126
Fragments arranged by size:
1126 1013 644 511 426 388 360 314
245
NaeI GCC'GGC
Cuts at: 209 3208 209
Size: 2999 2028
NarI GG'CG_CC
Cuts at: 2458 2706 2458
Size: 248 4779
NcoI C'CATG_G
Cuts at: 125 1121 125
Size: 996 4031
NgoMIV G'CCGG_C
Cuts at: 207 3206 207
Size: 2999 2028
NheI G'CTAG_C
Cuts at: 1177 1177
Size: 5027
NruI TCG'CGA
Cuts at: 727 727
Size: 5027
NspI r_CATG'y
Cuts at: 1461 2080 2580 3487 1461
Size: 619 500 907 3001
Fragments arranged by size:
3001 907 619 500
PciI A'CATG_T
Cuts at: 3483 3483
Size: 5027
PfoI T'CCnGG_A
Cuts at: 500 500
Size: 5027
PmeI GTTT'AAAC
Cuts at: 2026 2026
Size: 5027
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4166 4198 4166
Size: 32 4995
PpuMI rG'GwC_Cy
Cuts at: 236 236
Size: 5027
PstI C_TGCA'G
Cuts at: 572 876 1203 1499 2074 2759 572
Size: 304 327 296 575 685 2840
Fragments arranged by size:
2840 685 575 327 304 296
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5027
PvuII CAG'CTG
Cuts at: 1494 2452 2812 1494
Size: 958 360 3709
Fragments arranged by size:
3709 958 360
RsrII CG'GwC_CG
Cuts at: 3222 3222
Size: 5027
SacI G_AGCT'C
Cuts at: 2041 2041
Size: 5027
SalI G'TCGA_C
Cuts at: 2064 2064
Size: 5027
SapI GCTCTTCn'nnn_
Cuts at: 3050 3260 3050
Size: 210 4817
SbfI CC_TGCA'GG
Cuts at: 572 2074 572
Size: 1502 3525
ScaI AGT'ACT
Cuts at: 1981 4544 1981
Size: 2563 2464
SfcI C'TryA_G
Cuts at: 33 568 872 1142 1199 1495 1907 2070 2755
Size: 535 304 270 57 296 412 163 685
Cuts at: 2755 3748 3939 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 535 412 304 296 270
191 163 57
SfoI GGC'GCC
Cuts at: 2459 2707 2459
Size: 248 4779
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5027
SmaI CCC'GGG
Cuts at: 2049 2049
Size: 5027
SmlI C'TyrA_G
Cuts at: 1046 2182 3097 3589 3851 1046
Size: 1136 915 492 262 2222
Fragments arranged by size:
2222 1136 915 492 262
SnaBI TAC'GTA
Cuts at: 2019 2019
Size: 5027
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5027
SphI G_CATG'C
Cuts at: 2080 2580 2080
Size: 500 4527
SspI AAT'ATT
Cuts at: 1995 1995
Size: 5027
StuI AGG'CCT
Cuts at: 1052 1052
Size: 5027
StyI C'CwwG_G
Cuts at: 125 1121 2161 2515 125
Size: 996 1040 354 2637
Fragments arranged by size:
2637 1040 996 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 371 2610 2743 3202 3411 371
Size: 2239 133 459 209 1987
Fragments arranged by size:
2239 1987 459 209 133
TatI w'GTAC_w
Cuts at: 533 1979 4542 533
Size: 1446 2563 1018
Fragments arranged by size:
2563 1446 1018
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4544 4576 4544
Size: 32 4995
Tth111I GACn'n_nGTC
Cuts at: 2824 2824
Size: 5027
XbaI T'CTAG_A
Cuts at: 2058 2058
Size: 5027
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2522 2522
Size: 5027
XmaI C'CCGG_G
Cuts at: 2047 2047
Size: 5027
ZraI GAC'GTC
Cuts at: 978 978
Size: 5027
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NruI NspI PciI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI Bpu10I BsgI
BsiWI BsmI BsrGI BssHII BstEII Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NotI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI SwaI XhoI XmnI