Restriction map for
pF1KSDA1036
(Circular) (Six-base) MAPSORT of: pF1KSDA1036.seq Check: 2483 from: 1 to: 4234
pF1KSDA1036 4234 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 469 1220 1270 469
Size: 751 50 3433
Fragments arranged by size:
3433 751 50
Acc65I G'GTAC_C
Cuts at: 1205 1248 1205
Size: 43 4191
AclI AA'CG_TT
Cuts at: 4074 4074
Size: 4234
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2057 2489 3238 3506 2057
Size: 432 749 268 2785
Fragments arranged by size:
2785 749 432 268
AfeI AGC'GCT
Cuts at: 505 688 505
Size: 183 4051
AflIII A'CryG_T
Cuts at: 363 1584 2690 3667 363
Size: 1221 1106 977 930
Fragments arranged by size:
1221 1106 977 930
AgeI A'CCGG_T
Cuts at: 2622 2622
Size: 4234
AlwNI CAG_nnn'CTG
Cuts at: 1537 3106 1537
Size: 1569 2665
ApaI G_GGCC'C
Cuts at: 1057 1110 1057
Size: 53 4181
ApaLI G'TGCA_C
Cuts at: 3004 3004
Size: 4234
ApoI r'AATT_y
Cuts at: 653 1236 3587 3843 653
Size: 583 2351 256 1044
Fragments arranged by size:
2351 1044 583 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4234
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4234
AvaI C'yCGr_G
Cuts at: 1155 1252 1155
Size: 97 4137
BamHI G'GATC_C
Cuts at: 407 1257 407
Size: 850 3384
BanI G'GyrC_C
Cuts at: 166 1205 1248 1662 1910 1945 166
Size: 1039 43 414 248 35 2455
Fragments arranged by size:
2455 1039 414 248 43 35
BanII G_rGCy'C
Cuts at: 842 1057 1110 1181 1246 2276 842
Size: 215 53 71 65 1030 2800
Fragments arranged by size:
2800 1030 215 71 65 53
BbeI G_GCGC'C
Cuts at: 1666 1914 1666
Size: 248 3986
BbsI GAAGACnn'nnnn_
Cuts at: 1174 1174
Size: 4234
BbvCI CC'TCA_GC
Cuts at: 237 237
Size: 4234
BciVI GTATCCnnnnn_n'
Cuts at: 2130 2893 4234 2130
Size: 763 1341 2130
Fragments arranged by size:
2130 1341 763
BclI T'GATC_A
Cuts at: 1752 1752
Size: 4234
BglII A'GATC_T
Cuts at: 1747 3786 1747
Size: 2039 2195
BlpI GC'TnA_GC
Cuts at: 832 1344 832
Size: 512 3722
Bme1580I G_kGCm'C
Cuts at: 1057 1110 1857 1950 3008 1057
Size: 53 747 93 1058 2283
Fragments arranged by size:
2283 1058 747 93 53
BmgBI CAC'GTC
Cuts at: 969 969
Size: 4234
BmrI ACTGGGnnnn_n'
Cuts at: 1104 1444 1525 1860 4006 1104
Size: 340 81 335 2146 1332
Fragments arranged by size:
2146 1332 340 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 587 2599 587
Size: 2012 2222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 1027 703
Size: 324 3910
Bpu10I CC'TnA_GC
Cuts at: 237 482 237
Size: 245 3989
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 871 1018 1408 2287 2781 3079 871
Size: 147 390 879 494 298 2026
Fragments arranged by size:
2026 879 494 390 298 147
BsaI GGTCTCn'nnnn_
Cuts at: 214 3938 214
Size: 3724 510
BsaAI yAC'GTr
Cuts at: 366 882 1224 1585 366
Size: 516 342 361 3015
Fragments arranged by size:
3015 516 361 342
BsaBI GATnn'nnATC
Cuts at: 6 1746 3785 6
Size: 1740 2039 455
Fragments arranged by size:
2039 1740 455
BsaHI Gr'CG_yC
Cuts at: 296 1663 1911 2615 4111 296
Size: 1367 248 704 1496 419
Fragments arranged by size:
1496 1367 704 419 248
BsaWI w'CCGG_w
Cuts at: 1076 1421 1942 2622 2896 3043 3454 1076
Size: 345 521 680 274 147 411 1856
Fragments arranged by size:
1856 680 521 411 345 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 380 410 1041 1071 380
Size: 30 631 30 3543
Fragments arranged by size:
3543 631 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 268 268
Size: 4234
BseYI C'CCAG_C
Cuts at: 148 744 777 900 1657 2415 2994 3490 148
Size: 596 33 123 757 758 579 496 892
Fragments arranged by size:
892 758 757 596 579 496 123 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 520
Size: 4234
BsiEI CG_ry'CG
Cuts at: 104 870 1774 1820 3030 104
Size: 766 904 46 1210 1308
Fragments arranged by size:
1308 1210 904 766 46
BsiHKAI G_wGCw'C
Cuts at: 842 887 1246 2024 2214 3008 842
Size: 45 359 778 190 794 2068
Fragments arranged by size:
2068 794 778 359 190 45
BsmBI CGTCTCn'nnnn_
Cuts at: 281 281
Size: 4234
Bsp1286I G_dGCh'C
Cuts at: 842 887 1057 1110 1181 1246 1857 1950 2024
Size: 45 170 53 71 65 611 93 74
Cuts at: 2024 2214 2276 3008 842
Size: 190 62 732 2068
Fragments arranged by size:
2068 732 611 190 170 93 74 71
65 62 53 45
BspEI T'CCGG_A
Cuts at: 3454 3454
Size: 4234
BspHI T'CATG_A
Cuts at: 3410 3410
Size: 4234
BspMI ACCTGCnnnn'nnnn_
Cuts at: 290 434 1282 1496 1798 2179 290
Size: 144 848 214 302 381 2345
Fragments arranged by size:
2345 848 381 302 214 144
BsrBI CCG'CTC
Cuts at: 50 2524 2578 4064 4229 50
Size: 2474 54 1486 165 55
Fragments arranged by size:
2474 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2144 2144
Size: 4234
BsrFI r'CCGG_y
Cuts at: 2230 2411 2622 2230
Size: 181 211 3842
Fragments arranged by size:
3842 211 181
BsrGI T'GTAC_A
Cuts at: 806 806
Size: 4234
BssSI C'ACGA_G
Cuts at: 841 2503 2863 841
Size: 1662 360 2212
Fragments arranged by size:
2212 1662 360
BstAPI GCAn_nnn'nTGC
Cuts at: 960 1609 960
Size: 649 3585
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4234
BstXI CCAn_nnnn'nTGG
Cuts at: 385 385
Size: 4234
BstYI r'GATC_y
Cuts at: 1 374 407 434 1211 1257 1724 1747 2082
Size: 373 33 27 777 46 467 23 335
Cuts at: 2082 2328 3342 3428 3440 3786 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1014 777 467 449 373 346 335 246
86 46 33 27 23 12
BtgI C'CryG_G
Cuts at: 411 411
Size: 4234
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1001 2182 2363 2526 1001
Size: 1181 181 163 2709
Fragments arranged by size:
2709 1181 181 163
BtsI GCAGTG_nn'
Cuts at: 1146 1475 1146
Size: 329 3905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 300 335 300
Size: 35 4199
DraI TTT'AAA
Cuts at: 1231 1231
Size: 4234
DraIII CAC_nnn'GTG
Cuts at: 741 933 960 741
Size: 192 27 4015
Fragments arranged by size:
4015 192 27
DrdI GACnn_nn'nnGTC
Cuts at: 295 1938 2798 295
Size: 1643 860 1731
Fragments arranged by size:
1731 1643 860
EaeI y'GGCC_r
Cuts at: 188 368 593 867 1817 1991 2382 2409 3756
Size: 180 225 274 950 174 391 27 1347
Cuts at: 3756 188
Size: 666
Fragments arranged by size:
1347 950 666 391 274 225 180 174
27
EagI C'GGCC_G
Cuts at: 867 1817 867
Size: 950 3284
EarI CTCTTCn'nnn_
Cuts at: 257 269 556 2255 2465 257
Size: 12 287 1699 210 2026
Fragments arranged by size:
2026 1699 287 210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 721 2440 2752 2898 4044 721
Size: 1719 312 146 1146 911
Fragments arranged by size:
1719 1146 911 312 146
EcoICRI GAG'CTC
Cuts at: 840 1244 840
Size: 404 3830
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 1027 2057 2489 3238 3506 703
Size: 324 1030 432 749 268 1431
Fragments arranged by size:
1431 1030 749 432 324 268
EcoNI CCTnn'n_nnAGG
Cuts at: 348 575 595 348
Size: 227 20 3987
Fragments arranged by size:
3987 227 20
EcoO109I rG'GnC_Cy
Cuts at: 279 615 798 1054 1371 279
Size: 336 183 256 317 3142
Fragments arranged by size:
3142 336 317 256 183
EcoRI G'AATT_C
Cuts at: 1236 1236
Size: 4234
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 729 2251 729
Size: 1522 2712
FspI TGC'GCA
Cuts at: 2013 2013
Size: 4234
HaeII r_GCGC'y
Cuts at: 489 507 690 978 1014 1666 1914 2938 3917
Size: 18 183 288 36 652 248 1024 979
Cuts at: 3917 489
Size: 806
Fragments arranged by size:
1024 979 806 652 288 248 183 36
18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1041 1073 2074 2104 2106 2136 1041
Size: 32 1001 30 2 30 3139
Fragments arranged by size:
3139 1001 32 30 30 2
HincII GTy'rAC
Cuts at: 312 1271 312
Size: 959 3275
KasI G'GCGC_C
Cuts at: 1662 1910 1662
Size: 248 3986
KpnI G_GTAC'C
Cuts at: 1209 1252 1209
Size: 43 4191
MluI A'CGCG_T
Cuts at: 3667 3667
Size: 4234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 199 2905 3089 199
Size: 2706 184 1344
Fragments arranged by size:
2706 1344 184
MscI TGG'CCA
Cuts at: 370 595 1993 370
Size: 225 1398 2611
Fragments arranged by size:
2611 1398 225
MslI CAynn'nnrTG
Cuts at: 383 2221 2348 383
Size: 1838 127 2269
Fragments arranged by size:
2269 1838 127
MspA1I CmG'CkG
Cuts at: 164 184 872 1104 1343 1657 2017 3032 3277
Size: 20 688 232 239 314 360 1015 245
Cuts at: 3277 164
Size: 1121
Fragments arranged by size:
1121 1015 688 360 314 245 239 232
20
NaeI GCC'GGC
Cuts at: 2413 2413
Size: 4234
NarI GG'CG_CC
Cuts at: 1663 1911 1663
Size: 248 3986
NgoMIV G'CCGG_C
Cuts at: 2411 2411
Size: 4234
NruI TCG'CGA
Cuts at: 2595 2595
Size: 4234
NspI r_CATG'y
Cuts at: 675 1029 1285 1785 2694 675
Size: 354 256 500 909 2215
Fragments arranged by size:
2215 909 500 354 256
PciI A'CATG_T
Cuts at: 2690 2690
Size: 4234
PmeI GTTT'AAAC
Cuts at: 1231 1231
Size: 4234
PmlI CAC'GTG
Cuts at: 366 882 366
Size: 516 3718
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3373 3405 3373
Size: 32 4202
PpuMI rG'GwC_Cy
Cuts at: 279 798 279
Size: 519 3715
PspOMI G'GGCC_C
Cuts at: 1053 1106 1053
Size: 53 4181
PstI C_TGCA'G
Cuts at: 524 1279 1964 524
Size: 755 685 2794
Fragments arranged by size:
2794 755 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4234
PvuII CAG'CTG
Cuts at: 1657 2017 1657
Size: 360 3874
RsrII CG'GwC_CG
Cuts at: 2427 2427
Size: 4234
SacI G_AGCT'C
Cuts at: 842 1246 842
Size: 404 3830
SalI G'TCGA_C
Cuts at: 1269 1269
Size: 4234
SapI GCTCTTCn'nnn_
Cuts at: 556 2255 2465 556
Size: 1699 210 2325
Fragments arranged by size:
2325 1699 210
SbfI CC_TGCA'GG
Cuts at: 1279 1279
Size: 4234
ScaI AGT'ACT
Cuts at: 3751 3751
Size: 4234
SfcI C'TryA_G
Cuts at: 33 520 1275 1960 2955 3146 33
Size: 487 755 685 995 191 1121
Fragments arranged by size:
1121 995 755 685 487 191
SfoI GGC'GCC
Cuts at: 1664 1912 1664
Size: 248 3986
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4234
SmaI CCC'GGG
Cuts at: 1254 1254
Size: 4234
SmlI C'TyrA_G
Cuts at: 886 1033 1387 2302 2796 3058 886
Size: 147 354 915 494 262 2062
Fragments arranged by size:
2062 915 494 354 262 147
SnaBI TAC'GTA
Cuts at: 1224 1224
Size: 4234
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4234
SphI G_CATG'C
Cuts at: 675 1285 1785 675
Size: 610 500 3124
Fragments arranged by size:
3124 610 500
StyI C'CwwG_G
Cuts at: 596 1070 1366 1720 596
Size: 474 296 354 3110
Fragments arranged by size:
3110 474 354 296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1815 1948 2407 2618 1815
Size: 133 459 211 3431
Fragments arranged by size:
3431 459 211 133
TatI w'GTAC_w
Cuts at: 524 806 3749 524
Size: 282 2943 1009
Fragments arranged by size:
2943 1009 282
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 340 372 380 412 3751 3783 340
Size: 32 8 32 3339 32 791
Fragments arranged by size:
3339 791 32 32 32 8
Tth111I GACn'n_nGTC
Cuts at: 2029 2029
Size: 4234
XbaI T'CTAG_A
Cuts at: 1263 1263
Size: 4234
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1727 1727
Size: 4234
XmaI C'CCGG_G
Cuts at: 1252 1252
Size: 4234
XmnI GAAnn'nnTTC
Cuts at: 727 727
Size: 4234
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NruI
NspI PciI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BglI BmtI BsiWI BsmI
BssHII BstEII BstZ17I Bsu36I ClaI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NsiI
PacI PasI PflMI PfoI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI SspI StuI
SwaI XhoI ZraI