Restriction map for
pF1KSDA1029
(Circular) (Six-base) MAPSORT of: pF1KSDA1029.seq Check: 4325 from: 1 to: 5848
pF1KSDA1029 5848 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 788 2884 788
Size: 2096 3752
Acc65I G'GTAC_C
Cuts at: 195 1470 2862 195
Size: 1275 1392 3181
Fragments arranged by size:
3181 1392 1275
AclI AA'CG_TT
Cuts at: 5688 5688
Size: 5848
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1207 3671 4103 4852 5120 1207
Size: 2464 432 749 268 1935
Fragments arranged by size:
2464 1935 749 432 268
AfeI AGC'GCT
Cuts at: 2329 2329
Size: 5848
AflIII A'CryG_T
Cuts at: 2702 3198 4304 5281 2702
Size: 496 1106 977 3269
Fragments arranged by size:
3269 1106 977 496
AgeI A'CCGG_T
Cuts at: 4236 4236
Size: 5848
AhdI GACnn_n'nnGTC
Cuts at: 1173 1173
Size: 5848
AleI CACnn'nnGTG
Cuts at: 727 2032 727
Size: 1305 4543
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2045 2045
Size: 5848
AlwNI CAG_nnn'CTG
Cuts at: 450 514 3151 4720 450
Size: 64 2637 1569 1578
Fragments arranged by size:
2637 1578 1569 64
ApaI G_GGCC'C
Cuts at: 2245 2245
Size: 5848
ApaLI G'TGCA_C
Cuts at: 4618 4618
Size: 5848
ApoI r'AATT_y
Cuts at: 474 2850 5201 5457 474
Size: 2376 2351 256 865
Fragments arranged by size:
2376 2351 865 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5848
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5848
AvaI C'yCGr_G
Cuts at: 1702 2093 2238 2866 1702
Size: 391 145 628 4684
Fragments arranged by size:
4684 628 391 145
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1462 1495 1462
Size: 33 5815
BamHI G'GATC_C
Cuts at: 857 2871 857
Size: 2014 3834
BanI G'GyrC_C
Cuts at: 161 195 558 864 874 1470 1920 2452 2487
Size: 34 363 306 10 596 450 532 35
Cuts at: 2487 2862 3276 3524 3559 161
Size: 375 414 248 35 2450
Fragments arranged by size:
2450 596 532 450 414 375 363 306
248 35 35 34 10
BanII G_rGCy'C
Cuts at: 898 970 1385 1574 1802 1944 2041 2239 2245
Size: 72 415 189 228 142 97 198 6
Cuts at: 2245 2605 2647 2860 3890 898
Size: 360 42 213 1030 2856
Fragments arranged by size:
2856 1030 415 360 228 213 198 189
142 97 72 42 6
BbeI G_GCGC'C
Cuts at: 868 2456 2491 3280 3528 868
Size: 1588 35 789 248 3188
Fragments arranged by size:
3188 1588 789 248 35
BbsI GAAGACnn'nnnn_
Cuts at: 1169 1610 1169
Size: 441 5407
BbvCI CC'TCA_GC
Cuts at: 305 566 305
Size: 261 5587
BciVI GTATCCnnnnn_n'
Cuts at: 1166 3744 4507 5848 1166
Size: 2578 763 1341 1166
Fragments arranged by size:
2578 1341 1166 763
BclI T'GATC_A
Cuts at: 3366 3366
Size: 5848
BglI GCCn_nnn'nGGC
Cuts at: 1114 1705 2104 2225 2664 1114
Size: 591 399 121 439 4298
Fragments arranged by size:
4298 591 439 399 121
BglII A'GATC_T
Cuts at: 3361 5400 3361
Size: 2039 3809
BlpI GC'TnA_GC
Cuts at: 1568 2958 1568
Size: 1390 4458
Bme1580I G_kGCm'C
Cuts at: 877 1925 2245 3471 3564 4622 877
Size: 1048 320 1226 93 1058 2103
Fragments arranged by size:
2103 1226 1058 1048 320 93
BmgBI CAC'GTC
Cuts at: 2703 2703
Size: 5848
BmrI ACTGGGnnnn_n'
Cuts at: 347 944 1402 3058 3139 3474 5620 347
Size: 597 458 1656 81 335 2146 575
Fragments arranged by size:
2146 1656 597 575 458 335 81
BmtI G_CTAG'C
Cuts at: 152 152
Size: 5848
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 153 1151 1500 1588 4213 153
Size: 998 349 88 2625 1788
Fragments arranged by size:
2625 1788 998 349 88
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 284 596 621 648 1246 2061 2723 187
Size: 97 312 25 27 598 815 662 3312
Fragments arranged by size:
3312 815 662 598 312 97 27 25
Bpu10I CC'TnA_GC
Cuts at: 305 566 2150 305
Size: 261 1584 4003
Fragments arranged by size:
4003 1584 261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1477 2779 3022 3901 4395 4693 1477
Size: 1302 243 879 494 298 2632
Fragments arranged by size:
2632 1302 879 494 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 1087 1136 1878 2093 2347 5552 1087
Size: 49 742 215 254 3205 1383
Fragments arranged by size:
3205 1383 742 254 215 49
BsaAI yAC'GTr
Cuts at: 2838 3199 2838
Size: 361 5487
BsaBI GATnn'nnATC
Cuts at: 6 413 3360 5399 6
Size: 407 2947 2039 455
Fragments arranged by size:
2947 2039 455 407
BsaHI Gr'CG_yC
Cuts at: 865 1053 2191 2453 2488 3277 3525 4229 5725
Size: 188 1138 262 35 789 248 704 1496
Cuts at: 5725 865
Size: 988
Fragments arranged by size:
1496 1138 988 789 704 262 248 188
35
BsaWI w'CCGG_w
Cuts at: 3035 3556 4236 4510 4657 5068 3035
Size: 521 680 274 147 411 3815
Fragments arranged by size:
3815 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 199 583 906 1124 1243 1390 1393 1477
Size: 42 384 323 218 119 147 3 84
Cuts at: 1477 1971 157
Size: 494 4034
Fragments arranged by size:
4034 494 384 323 218 147 119 84
42 3
BseYI C'CCAG_C
Cuts at: 275 318 526 658 672 941 1159 1731 1903
Size: 43 208 132 14 269 218 572 172
Cuts at: 1903 2212 2228 2371 3271 4029 4608 5104 275
Size: 309 16 143 900 758 579 496 1019
Fragments arranged by size:
1019 900 758 579 572 496 309 269
218 208 172 143 132 43 16 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 1058 2613 2811 235
Size: 823 1555 198 3272
Fragments arranged by size:
3272 1555 823 198
BsiEI CG_ry'CG
Cuts at: 104 2446 3388 3434 4644 104
Size: 2342 942 46 1210 1308
Fragments arranged by size:
2342 1308 1210 942 46
BsiHKAI G_wGCw'C
Cuts at: 1574 2860 3638 3828 4622 1574
Size: 1286 778 190 794 2800
Fragments arranged by size:
2800 1286 794 778 190
BsmI GAATG_Cn'
Cuts at: 228 1220 228
Size: 992 4856
BsmBI CGTCTCn'nnnn_
Cuts at: 903 1044 903
Size: 141 5707
Bsp1286I G_dGCh'C
Cuts at: 877 898 970 1385 1574 1802 1925 1944 2041
Size: 21 72 415 189 228 123 19 97
Cuts at: 2041 2239 2245 2605 2647 2860 3471 3564 3638
Size: 198 6 360 42 213 611 93 74
Cuts at: 3638 3828 3890 4622 877
Size: 190 62 732 2103
Fragments arranged by size:
2103 732 611 415 360 228 213 198
190 189 123 97 93 74 72 62
42 21 19 6
BspEI T'CCGG_A
Cuts at: 5068 5068
Size: 5848
BspHI T'CATG_A
Cuts at: 5024 5024
Size: 5848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1778 1937 2896 3110 3412 3793 1778
Size: 159 959 214 302 381 3833
Fragments arranged by size:
3833 959 381 302 214 159
BsrBI CCG'CTC
Cuts at: 50 2684 4138 4192 5678 5843 50
Size: 2634 1454 54 1486 165 55
Fragments arranged by size:
2634 1486 1454 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3758 3758
Size: 5848
BsrFI r'CCGG_y
Cuts at: 288 426 561 2614 3844 4025 4236 288
Size: 138 135 2053 1230 181 211 1900
Fragments arranged by size:
2053 1900 1230 211 181 138 135
BssSI C'ACGA_G
Cuts at: 1433 2316 4117 4477 1433
Size: 883 1801 360 2804
Fragments arranged by size:
2804 1801 883 360
BstAPI GCAn_nnn'nTGC
Cuts at: 3223 3223
Size: 5848
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5848
BstEII G'GTnAC_C
Cuts at: 1290 1290
Size: 5848
BstXI CCAn_nnnn'nTGG
Cuts at: 885 1911 885
Size: 1026 4822
BstYI r'GATC_y
Cuts at: 1 857 1314 2871 3338 3361 3696 3942 4956
Size: 856 457 1557 467 23 335 246 1014
Cuts at: 4956 5042 5054 5400 1
Size: 86 12 346 449
Fragments arranged by size:
1557 1014 856 467 457 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 125 1004 1451 1761 2245 2440 2464 2596 2690
Size: 879 447 310 484 195 24 132 94
Cuts at: 2690 2769 125
Size: 79 3204
Fragments arranged by size:
3204 879 484 447 310 195 132 94
79 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3796 3977 4140 3796
Size: 181 163 5504
Fragments arranged by size:
5504 181 163
BtsI GCAGTG_nn'
Cuts at: 229 1070 2027 3089 229
Size: 841 957 1062 2988
Fragments arranged by size:
2988 1062 957 841
DraI TTT'AAA
Cuts at: 2845 2845
Size: 5848
DraIII CAC_nnn'GTG
Cuts at: 536 1435 536
Size: 899 4949
DrdI GACnn_nn'nnGTC
Cuts at: 3552 4412 3552
Size: 860 4988
EaeI y'GGCC_r
Cuts at: 176 812 842 973 2007 3431 3605 3996 4023
Size: 636 30 131 1034 1424 174 391 27
Cuts at: 4023 5370 176
Size: 1347 654
Fragments arranged by size:
1424 1347 1034 654 636 391 174 131
30 27
EagI C'GGCC_G
Cuts at: 3431 3431
Size: 5848
EarI CTCTTCn'nnn_
Cuts at: 185 3869 4079 185
Size: 3684 210 1954
Fragments arranged by size:
3684 1954 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 386 1474 4054 4366 4512 5658 386
Size: 1088 2580 312 146 1146 576
Fragments arranged by size:
2580 1146 1088 576 312 146
EcoICRI GAG'CTC
Cuts at: 1572 2858 1572
Size: 1286 4562
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 284 596 621 648 1207 1246 2061 2723
Size: 97 312 25 27 559 39 815 662
Cuts at: 2723 3671 4103 4852 5120 187
Size: 948 432 749 268 915
Fragments arranged by size:
948 915 815 749 662 559 432 312
268 97 39 27 25
EcoNI CCTnn'n_nnAGG
Cuts at: 169 1125 1546 1933 169
Size: 956 421 387 4084
Fragments arranged by size:
4084 956 421 387
EcoO109I rG'GnC_Cy
Cuts at: 511 668 930 1706 1785 2106 2114 2666 2985
Size: 157 262 776 79 321 8 552 319
Cuts at: 2985 511
Size: 3374
Fragments arranged by size:
3374 776 552 321 319 262 157 79
8
EcoRI G'AATT_C
Cuts at: 474 2850 474
Size: 2376 3472
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3865 3865
Size: 5848
FspI TGC'GCA
Cuts at: 3627 3627
Size: 5848
HaeII r_GCGC'y
Cuts at: 868 2331 2392 2435 2456 2491 3280 3528 4552
Size: 1463 61 43 21 35 789 248 1024
Cuts at: 4552 5531 868
Size: 979 1185
Fragments arranged by size:
1463 1185 1024 979 789 248 61 43
35 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 589 621 673 705 1084 1116 1159 1191 1284
Size: 32 52 32 379 32 43 32 93
Cuts at: 1284 1316 2133 2165 3688 3718 3720 3750 589
Size: 32 817 32 1523 30 2 30 2687
Fragments arranged by size:
2687 1523 817 379 93 52 43 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 333 834 2885 333
Size: 501 2051 3296
Fragments arranged by size:
3296 2051 501
KasI G'GCGC_C
Cuts at: 864 2452 2487 3276 3524 864
Size: 1588 35 789 248 3188
Fragments arranged by size:
3188 1588 789 248 35
KpnI G_GTAC'C
Cuts at: 199 1474 2866 199
Size: 1275 1392 3181
Fragments arranged by size:
3181 1392 1275
MluI A'CGCG_T
Cuts at: 5281 5281
Size: 5848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 492 1190 1433 2723 4519 4703 492
Size: 698 243 1290 1796 184 1637
Fragments arranged by size:
1796 1637 1290 698 243 184
MscI TGG'CCA
Cuts at: 178 814 3607 178
Size: 636 2793 2419
Fragments arranged by size:
2793 2419 636
MslI CAynn'nnrTG
Cuts at: 727 2032 3835 3962 727
Size: 1305 1803 127 2613
Fragments arranged by size:
2613 1803 1305 127
MspA1I CmG'CkG
Cuts at: 304 322 766 1330 1453 1646 1824 2289 2343
Size: 18 444 564 123 193 178 465 54
Cuts at: 2343 2382 2442 2466 2598 2649 2771 2957 3271
Size: 39 60 24 132 51 122 186 314
Cuts at: 3271 3631 4646 4891 304
Size: 360 1015 245 1261
Fragments arranged by size:
1261 1015 564 465 444 360 314 245
193 186 178 132 123 122 60 54
51 39 24 18
NaeI GCC'GGC
Cuts at: 4027 4027
Size: 5848
NarI GG'CG_CC
Cuts at: 865 2453 2488 3277 3525 865
Size: 1588 35 789 248 3188
Fragments arranged by size:
3188 1588 789 248 35
NcoI C'CATG_G
Cuts at: 125 1004 1761 125
Size: 879 757 4212
Fragments arranged by size:
4212 879 757
NgoMIV G'CCGG_C
Cuts at: 4025 4025
Size: 5848
NheI G'CTAG_C
Cuts at: 148 148
Size: 5848
NruI TCG'CGA
Cuts at: 4209 4209
Size: 5848
NspI r_CATG'y
Cuts at: 2532 2899 3399 4308 2532
Size: 367 500 909 4072
Fragments arranged by size:
4072 909 500 367
PciI A'CATG_T
Cuts at: 4304 4304
Size: 5848
PflMI CCAn_nnn'nTGG
Cuts at: 502 2084 502
Size: 1582 4266
PfoI T'CCnGG_A
Cuts at: 1678 1678
Size: 5848
PmeI GTTT'AAAC
Cuts at: 2845 2845
Size: 5848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4987 5019 4987
Size: 32 5816
PpuMI rG'GwC_Cy
Cuts at: 1785 1785
Size: 5848
PspOMI G'GGCC_C
Cuts at: 2241 2241
Size: 5848
PstI C_TGCA'G
Cuts at: 327 2024 2387 2893 3578 327
Size: 1697 363 506 685 2597
Fragments arranged by size:
2597 1697 685 506 363
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5848
PvuII CAG'CTG
Cuts at: 304 322 766 1824 2382 3271 3631 304
Size: 18 444 1058 558 889 360 2521
Fragments arranged by size:
2521 1058 889 558 444 360 18
RsrII CG'GwC_CG
Cuts at: 4041 4041
Size: 5848
SacI G_AGCT'C
Cuts at: 1574 2860 1574
Size: 1286 4562
SacII CC_GC'GG
Cuts at: 1454 2443 2467 2599 2772 1454
Size: 989 24 132 173 4530
Fragments arranged by size:
4530 989 173 132 24
SalI G'TCGA_C
Cuts at: 2883 2883
Size: 5848
SapI GCTCTTCn'nnn_
Cuts at: 3869 4079 3869
Size: 210 5638
SbfI CC_TGCA'GG
Cuts at: 2893 2893
Size: 5848
ScaI AGT'ACT
Cuts at: 5365 5365
Size: 5848
SexAI A'CCwGG_T
Cuts at: 1916 1916
Size: 5848
SfcI C'TryA_G
Cuts at: 33 323 789 2020 2383 2889 3574 4569 4760
Size: 290 466 1231 363 506 685 995 191
Cuts at: 4760 33
Size: 1121
Fragments arranged by size:
1231 1121 995 685 506 466 363 290
191
SfoI GGC'GCC
Cuts at: 866 2454 2489 3278 3526 866
Size: 1588 35 789 248 3188
Fragments arranged by size:
3188 1588 789 248 35
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5848
SmaI CCC'GGG
Cuts at: 1704 2240 2868 1704
Size: 536 628 4684
Fragments arranged by size:
4684 628 536
SmlI C'TyrA_G
Cuts at: 1492 2794 3001 3916 4410 4672 1492
Size: 1302 207 915 494 262 2668
Fragments arranged by size:
2668 1302 915 494 262 207
SnaBI TAC'GTA
Cuts at: 2838 2838
Size: 5848
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5848
SphI G_CATG'C
Cuts at: 2532 2899 3399 2532
Size: 367 500 4981
Fragments arranged by size:
4981 500 367
SrfI GCCC'GGGC
Cuts at: 2240 2240
Size: 5848
StuI AGG'CCT
Cuts at: 849 1405 1552 2821 849
Size: 556 147 1269 3876
Fragments arranged by size:
3876 1269 556 147
StyI C'CwwG_G
Cuts at: 125 782 1004 1058 1286 1761 1868 1910 1934
Size: 657 222 54 228 475 107 42 24
Cuts at: 1934 2980 3334 125
Size: 1046 354 2639
Fragments arranged by size:
2639 1046 657 475 354 228 222 107
54 42 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1113 2220 2371 3429 3562 4021 4232 1113
Size: 1107 151 1058 133 459 211 2729
Fragments arranged by size:
2729 1107 1058 459 211 151 133
TatI w'GTAC_w
Cuts at: 5363 5363
Size: 5848
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 976 1008 1490 1522 5365 5397 976
Size: 32 482 32 3843 32 1427
Fragments arranged by size:
3843 1427 482 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1098 3643 1098
Size: 2545 3303
XbaI T'CTAG_A
Cuts at: 2877 2877
Size: 5848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 726 1110 3341 726
Size: 384 2231 3233
Fragments arranged by size:
3233 2231 384
XmaI C'CCGG_G
Cuts at: 1702 2238 2866 1702
Size: 536 628 4684
Fragments arranged by size:
4684 628 536
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NruI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AloI AscI AvrII BcgI BfrBI
BsaXI BsiWI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI
NsiI PacI PasI PmlI PshAI PsiI PspXI PsrI
SanDI SfiI SgrAI SspI SwaI XhoI XmnI ZraI