Restriction map for
pF1KSDA1028
(Circular) (Six-base) MAPSORT of: pF1KSDA1028.seq Check: 982 from: 1 to: 4643
pF1KSDA1028 4643 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1342 1342
Size: 4643
AccI GT'mk_AC
Cuts at: 230 1681 230
Size: 1451 3192
Acc65I G'GTAC_C
Cuts at: 1659 1659
Size: 4643
AclI AA'CG_TT
Cuts at: 4483 4483
Size: 4643
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1045 1238 1442 2468 2900 3647 3915 1045
Size: 193 204 1026 432 747 268 1773
Fragments arranged by size:
1773 1026 747 432 268 204 193
AflIII A'CryG_T
Cuts at: 1995 3099 4076 1995
Size: 1104 977 2562
Fragments arranged by size:
2562 1104 977
AgeI A'CCGG_T
Cuts at: 1625 3031 1625
Size: 1406 3237
AlwNI CAG_nnn'CTG
Cuts at: 1948 3515 1948
Size: 1567 3076
ApaLI G'TGCA_C
Cuts at: 3413 3413
Size: 4643
ApoI r'AATT_y
Cuts at: 1195 1647 3996 4252 1195
Size: 452 2349 256 1586
Fragments arranged by size:
2349 1586 452 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4643
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4643
AvaI C'yCGr_G
Cuts at: 1663 1663
Size: 4643
BamHI G'GATC_C
Cuts at: 696 1080 1119 1543 1668 696
Size: 384 39 424 125 3671
Fragments arranged by size:
3671 424 384 125 39
BanI G'GyrC_C
Cuts at: 214 1308 1378 1425 1659 2073 2321 2356 214
Size: 1094 70 47 234 414 248 35 2501
Fragments arranged by size:
2501 1094 414 248 234 70 47 35
BanII G_rGCy'C
Cuts at: 729 1597 1657 2687 729
Size: 868 60 1030 2685
Fragments arranged by size:
2685 1030 868 60
BbeI G_GCGC'C
Cuts at: 1312 2077 2325 1312
Size: 765 248 3630
Fragments arranged by size:
3630 765 248
BciVI GTATCCnnnnn_n'
Cuts at: 550 2541 3302 4643 550
Size: 1991 761 1341 550
Fragments arranged by size:
1991 1341 761 550
BclI T'GATC_A
Cuts at: 873 2163 873
Size: 1290 3353
BfrBI ATG'CAT
Cuts at: 274 274
Size: 4643
BglII A'GATC_T
Cuts at: 499 2158 4195 499
Size: 1659 2037 947
Fragments arranged by size:
2037 1659 947
BlpI GC'TnA_GC
Cuts at: 1755 1755
Size: 4643
Bme1580I G_kGCm'C
Cuts at: 1381 2268 2361 3417 1381
Size: 887 93 1056 2607
Fragments arranged by size:
2607 1056 887 93
BmgBI CAC'GTC
Cuts at: 228 228
Size: 4643
BmrI ACTGGGnnnn_n'
Cuts at: 933 1855 1936 2271 4415 933
Size: 922 81 335 2144 1161
Fragments arranged by size:
2144 1161 922 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3010 3010
Size: 4643
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 713 1395 1491 713
Size: 682 96 3865
Fragments arranged by size:
3865 682 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1819 2698 3190 3488 1819
Size: 879 492 298 2974
Fragments arranged by size:
2974 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4347 4347
Size: 4643
BsaAI yAC'GTr
Cuts at: 1635 1996 1635
Size: 361 4282
BsaBI GATnn'nnATC
Cuts at: 6 695 2157 4194 6
Size: 689 1462 2037 455
Fragments arranged by size:
2037 1462 689 455
BsaHI Gr'CG_yC
Cuts at: 1309 2074 2322 3024 4520 1309
Size: 765 248 702 1496 1432
Fragments arranged by size:
1496 1432 765 702 248
BsaWI w'CCGG_w
Cuts at: 1381 1625 1832 2353 3031 3305 3452 3863 1381
Size: 244 207 521 678 274 147 411 2161
Fragments arranged by size:
2161 678 521 411 274 244 207 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 166 196 1263 1293 166
Size: 30 1067 30 3516
Fragments arranged by size:
3516 1067 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 662 662
Size: 4643
BseYI C'CCAG_C
Cuts at: 1360 2068 2826 3403 3899 1360
Size: 708 758 577 496 2104
Fragments arranged by size:
2104 758 708 577 496
BsiEI CG_ry'CG
Cuts at: 104 2185 2231 3439 104
Size: 2081 46 1208 1308
Fragments arranged by size:
2081 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1564 1597 1657 2435 2625 3417 1564
Size: 33 60 778 190 792 2790
Fragments arranged by size:
2790 792 778 190 60 33
BsmBI CGTCTCn'nnnn_
Cuts at: 1589 1589
Size: 4643
Bsp1286I G_dGCh'C
Cuts at: 729 1381 1564 1597 1657 2268 2361 2435 2625
Size: 652 183 33 60 611 93 74 190
Cuts at: 2625 2687 3417 729
Size: 62 730 1955
Fragments arranged by size:
1955 730 652 611 190 183 93 74
62 60 33
BspEI T'CCGG_A
Cuts at: 3863 3863
Size: 4643
BspHI T'CATG_A
Cuts at: 870 3819 870
Size: 2949 1694
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1342 1693 1907 2209 2590 1342
Size: 351 214 302 381 3395
Fragments arranged by size:
3395 381 351 302 214
BsrBI CCG'CTC
Cuts at: 50 162 168 2935 2989 4473 4638 50
Size: 112 6 2767 54 1484 165 55
Fragments arranged by size:
2767 1484 165 112 55 54 6
BsrDI GCAATG_nn'
Cuts at: 364 2555 364
Size: 2191 2452
BsrFI r'CCGG_y
Cuts at: 1165 1625 2641 2822 3031 1165
Size: 460 1016 181 209 2777
Fragments arranged by size:
2777 1016 460 209 181
BssSI C'ACGA_G
Cuts at: 2914 3272 2914
Size: 358 4285
BstAPI GCAn_nnn'nTGC
Cuts at: 1113 2020 1113
Size: 907 3736
BstBI TT'CG_AA
Cuts at: 110 3004 110
Size: 2894 1749
BstYI r'GATC_y
Cuts at: 1 499 696 1080 1119 1543 1668 2135 2158
Size: 498 197 384 39 424 125 467 23
Cuts at: 2158 2493 2739 3751 3837 3849 4195 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 498 467 449 424 384 346 335
246 197 125 86 39 23 12
BtgI C'CryG_G
Cuts at: 125 1076 1444 125
Size: 951 368 3324
Fragments arranged by size:
3324 951 368
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2593 2774 2937 2593
Size: 181 163 4299
Fragments arranged by size:
4299 181 163
BtsI GCAGTG_nn'
Cuts at: 1886 1886
Size: 4643
DraI TTT'AAA
Cuts at: 1642 1642
Size: 4643
DrdI GACnn_nn'nnGTC
Cuts at: 2349 3207 2349
Size: 858 3785
EaeI y'GGCC_r
Cuts at: 2228 2402 2793 2820 4165 2228
Size: 174 391 27 1345 2706
Fragments arranged by size:
2706 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2228 2228
Size: 4643
EarI CTCTTCn'nnn_
Cuts at: 1581 2666 2876 1581
Size: 1085 210 3348
Fragments arranged by size:
3348 1085 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 172 258 2851 3161 3307 4453 172
Size: 86 2593 310 146 1146 362
Fragments arranged by size:
2593 1146 362 310 146 86
EcoICRI GAG'CTC
Cuts at: 1595 1655 1595
Size: 60 4583
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 713 1045 1238 1395 1442 1491 2468 2900 3647
Size: 332 193 157 47 49 977 432 747
Cuts at: 3647 3915 713
Size: 268 1441
Fragments arranged by size:
1441 977 747 432 332 268 193 157
49 47
EcoNI CCTnn'n_nnAGG
Cuts at: 1420 1420
Size: 4643
EcoO109I rG'GnC_Cy
Cuts at: 613 1137 1453 1782 613
Size: 524 316 329 3474
Fragments arranged by size:
3474 524 329 316
EcoRI G'AATT_C
Cuts at: 1195 1647 1195
Size: 452 4191
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2662 2662
Size: 4643
FspI TGC'GCA
Cuts at: 2424 2424
Size: 4643
HaeII r_GCGC'y
Cuts at: 1312 2077 2325 3347 4326 1312
Size: 765 248 1022 979 1629
Fragments arranged by size:
1629 1022 979 765 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1065 1097 2485 2515 2517 2547 1065
Size: 32 1388 30 2 30 3161
Fragments arranged by size:
3161 1388 32 30 30 2
HincII GTy'rAC
Cuts at: 809 1682 809
Size: 873 3770
KasI G'GCGC_C
Cuts at: 1308 2073 2321 1308
Size: 765 248 3630
Fragments arranged by size:
3630 765 248
KpnI G_GTAC'C
Cuts at: 1663 1663
Size: 4643
MluI A'CGCG_T
Cuts at: 4076 4076
Size: 4643
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1108 3314 3498 1108
Size: 2206 184 2253
Fragments arranged by size:
2253 2206 184
MscI TGG'CCA
Cuts at: 2404 2404
Size: 4643
MslI CAynn'nnrTG
Cuts at: 353 2632 2759 353
Size: 2279 127 2237
Fragments arranged by size:
2279 2237 127
MspA1I CmG'CkG
Cuts at: 1754 2068 2428 3441 3686 1754
Size: 314 360 1013 245 2711
Fragments arranged by size:
2711 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1167 2824 1167
Size: 1657 2986
NarI GG'CG_CC
Cuts at: 1309 2074 2322 1309
Size: 765 248 3630
Fragments arranged by size:
3630 765 248
NcoI C'CATG_G
Cuts at: 125 1076 125
Size: 951 3692
NgoMIV G'CCGG_C
Cuts at: 1165 2822 1165
Size: 1657 2986
NsiI A_TGCA'T
Cuts at: 276 276
Size: 4643
NspI r_CATG'y
Cuts at: 1696 2196 3103 1696
Size: 500 907 3236
Fragments arranged by size:
3236 907 500
PciI A'CATG_T
Cuts at: 3099 3099
Size: 4643
PmeI GTTT'AAAC
Cuts at: 1642 1642
Size: 4643
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3782 3814 3782
Size: 32 4611
PpuMI rG'GwC_Cy
Cuts at: 613 1137 613
Size: 524 4119
PstI C_TGCA'G
Cuts at: 915 1118 1690 2375 915
Size: 203 572 685 3183
Fragments arranged by size:
3183 685 572 203
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4643
PvuII CAG'CTG
Cuts at: 2068 2428 2068
Size: 360 4283
RsrII CG'GwC_CG
Cuts at: 2838 2838
Size: 4643
SacI G_AGCT'C
Cuts at: 1597 1657 1597
Size: 60 4583
SalI G'TCGA_C
Cuts at: 1680 1680
Size: 4643
SapI GCTCTTCn'nnn_
Cuts at: 2666 2876 2666
Size: 210 4433
SbfI CC_TGCA'GG
Cuts at: 1690 1690
Size: 4643
ScaI AGT'ACT
Cuts at: 4160 4160
Size: 4643
SexAI A'CCwGG_T
Cuts at: 1421 1421
Size: 4643
SfcI C'TryA_G
Cuts at: 33 911 1114 1686 2371 3364 3555 33
Size: 878 203 572 685 993 191 1121
Fragments arranged by size:
1121 993 878 685 572 203 191
SfoI GGC'GCC
Cuts at: 1310 2075 2323 1310
Size: 765 248 3630
Fragments arranged by size:
3630 765 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4643
SmaI CCC'GGG
Cuts at: 1665 1665
Size: 4643
SmlI C'TyrA_G
Cuts at: 1798 2713 3205 3467 1798
Size: 915 492 262 2974
Fragments arranged by size:
2974 915 492 262
SnaBI TAC'GTA
Cuts at: 1635 1635
Size: 4643
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4643
SphI G_CATG'C
Cuts at: 1696 2196 1696
Size: 500 4143
StyI C'CwwG_G
Cuts at: 125 1076 1777 2131 125
Size: 951 701 354 2637
Fragments arranged by size:
2637 951 701 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 552 1348 1622 2226 2359 2818 3027 552
Size: 796 274 604 133 459 209 2168
Fragments arranged by size:
2168 796 604 459 274 209 133
TatI w'GTAC_w
Cuts at: 1010 4158 1010
Size: 3148 1495
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 879 911 1263 1295 1338 1370 4160 4192 879
Size: 32 352 32 43 32 2790 32 1330
Fragments arranged by size:
2790 1330 352 43 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2440 2440
Size: 4643
XbaI T'CTAG_A
Cuts at: 1674 1674
Size: 4643
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 532 1564 2138 532
Size: 1032 574 3037
Fragments arranged by size:
3037 1032 574
XmaI C'CCGG_G
Cuts at: 1663 1663
Size: 4643
XmnI GAAnn'nnTTC
Cuts at: 376 376
Size: 4643
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmtI
Bpu10I BsgI BsiWI BsmI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SrfI SspI StuI
SwaI XhoI ZraI