Restriction map for
pF1KSDA1024
(Circular) (Six-base) MAPSORT of: pF1KSDA1024.seq Check: 2924 from: 1 to: 5887
pF1KSDA1024 5887 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2487 2487
Size: 5887
AccI GT'mk_AC
Cuts at: 2923 2923
Size: 5887
Acc65I G'GTAC_C
Cuts at: 2901 2901
Size: 5887
AclI AA'CG_TT
Cuts at: 5727 5727
Size: 5887
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1921 3710 4142 4891 5159 1921
Size: 1789 432 749 268 2649
Fragments arranged by size:
2649 1789 749 432 268
AflIII A'CryG_T
Cuts at: 3237 4343 5320 3237
Size: 1106 977 3804
Fragments arranged by size:
3804 1106 977
AgeI A'CCGG_T
Cuts at: 4275 4275
Size: 5887
AleI CACnn'nnGTG
Cuts at: 1156 1729 1156
Size: 573 5314
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1939 1939
Size: 5887
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2485 2517 2485
Size: 32 5855
AlwNI CAG_nnn'CTG
Cuts at: 213 717 1062 1184 2321 3190 4759 213
Size: 504 345 122 1137 869 1569 1341
Fragments arranged by size:
1569 1341 1137 869 504 345 122
ApaI G_GGCC'C
Cuts at: 1151 1151
Size: 5887
ApaLI G'TGCA_C
Cuts at: 1596 4657 1596
Size: 3061 2826
ApoI r'AATT_y
Cuts at: 2773 2889 5240 5496 2773
Size: 116 2351 256 3164
Fragments arranged by size:
3164 2351 256 116
AseI AT'TA_AT
Cuts at: 20 20
Size: 5887
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5887
AvaI C'yCGr_G
Cuts at: 2905 2905
Size: 5887
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1726 1759 1726
Size: 33 5854
BamHI G'GATC_C
Cuts at: 1762 2910 1762
Size: 1148 4739
BanI G'GyrC_C
Cuts at: 1355 1549 2901 3315 3563 3598 1355
Size: 194 1352 414 248 35 3644
Fragments arranged by size:
3644 1352 414 248 194 35
BanII G_rGCy'C
Cuts at: 533 1151 1430 1577 2899 3929 533
Size: 618 279 147 1322 1030 2491
Fragments arranged by size:
2491 1322 1030 618 279 147
BbeI G_GCGC'C
Cuts at: 1359 3319 3567 1359
Size: 1960 248 3679
Fragments arranged by size:
3679 1960 248
BbsI GAAGACnn'nnnn_
Cuts at: 1909 2729 1909
Size: 820 5067
BbvCI CC'TCA_GC
Cuts at: 743 743
Size: 5887
BciVI GTATCCnnnnn_n'
Cuts at: 2734 3783 4546 5887 2734
Size: 1049 763 1341 2734
Fragments arranged by size:
2734 1341 1049 763
BclI T'GATC_A
Cuts at: 3405 3405
Size: 5887
BglII A'GATC_T
Cuts at: 164 506 1442 2058 3400 5439 164
Size: 342 936 616 1342 2039 612
Fragments arranged by size:
2039 1342 936 616 612 342
BlpI GC'TnA_GC
Cuts at: 1525 2997 1525
Size: 1472 4415
Bme1580I G_kGCm'C
Cuts at: 234 1151 1552 1600 2855 3510 3603 4661 234
Size: 917 401 48 1255 655 93 1058 1460
Fragments arranged by size:
1460 1255 1058 917 655 401 93 48
BmrI ACTGGGnnnn_n'
Cuts at: 935 3097 3178 3513 5659 935
Size: 2162 81 335 2146 1163
Fragments arranged by size:
2162 2146 1163 335 81
BmtI G_CTAG'C
Cuts at: 2186 2186
Size: 5887
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4252 4252
Size: 5887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1251 1483 1591 2720 1251
Size: 232 108 1129 4418
Fragments arranged by size:
4418 1129 232 108
Bpu10I CC'TnA_GC
Cuts at: 743 1805 1927 743
Size: 1062 122 4703
Fragments arranged by size:
4703 1062 122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2515 3061 3940 4434 4732 2515
Size: 546 879 494 298 3670
Fragments arranged by size:
3670 879 546 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 124 835 1583 5591 124
Size: 711 748 4008 420
Fragments arranged by size:
4008 748 711 420
BsaAI yAC'GTr
Cuts at: 1566 2877 3238 1566
Size: 1311 361 4215
Fragments arranged by size:
4215 1311 361
BsaBI GATnn'nnATC
Cuts at: 6 3399 5438 6
Size: 3393 2039 455
Fragments arranged by size:
3393 2039 455
BsaHI Gr'CG_yC
Cuts at: 1356 3316 3564 4268 5764 1356
Size: 1960 248 704 1496 1479
Fragments arranged by size:
1960 1496 1479 704 248
BsaWI w'CCGG_w
Cuts at: 3074 3595 4275 4549 4696 5107 3074
Size: 521 680 274 147 411 3854
Fragments arranged by size:
3854 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 796 826 2116 2146 2181 2211 2485 2509 2515
Size: 30 1290 30 35 30 274 24 6
Cuts at: 2515 2539 796
Size: 24 4144
Fragments arranged by size:
4144 1290 274 35 30 30 30 24
24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 874 1309 2281 2377 2380 2638 874
Size: 435 972 96 3 258 4123
Fragments arranged by size:
4123 972 435 258 96 3
BseYI C'CCAG_C
Cuts at: 1341 3310 4068 4647 5143 1341
Size: 1969 758 579 496 2085
Fragments arranged by size:
2085 1969 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1494 2071 2844 1494
Size: 577 773 4537
Fragments arranged by size:
4537 773 577
BsiEI CG_ry'CG
Cuts at: 104 3427 3473 4683 104
Size: 3323 46 1210 1308
Fragments arranged by size:
3323 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 275 980 1565 1600 1811 1928 2899 3677 3867
Size: 705 585 35 211 117 971 778 190
Cuts at: 3867 4661 275
Size: 794 1501
Fragments arranged by size:
1501 971 794 778 705 585 211 190
117 35
BsmI GAATG_Cn'
Cuts at: 2643 2643
Size: 5887
Bsp1286I G_dGCh'C
Cuts at: 234 275 533 980 1151 1430 1552 1565 1577
Size: 41 258 447 171 279 122 13 12
Cuts at: 1577 1600 1811 1928 2855 2899 3510 3603 3677
Size: 23 211 117 927 44 611 93 74
Cuts at: 3677 3867 3929 4661 234
Size: 190 62 732 1460
Fragments arranged by size:
1460 927 732 611 447 279 258 211
190 171 122 117 93 74 62 44
41 23 13 12
BspEI T'CCGG_A
Cuts at: 5107 5107
Size: 5887
BspHI T'CATG_A
Cuts at: 5063 5063
Size: 5887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2487 2681 2935 3149 3451 3832 2487
Size: 194 254 214 302 381 4542
Fragments arranged by size:
4542 381 302 254 214 194
BsrBI CCG'CTC
Cuts at: 50 4177 4231 5717 5882 50
Size: 4127 54 1486 165 55
Fragments arranged by size:
4127 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 519 1400 3797 519
Size: 881 2397 2609
Fragments arranged by size:
2609 2397 881
BsrFI r'CCGG_y
Cuts at: 1865 3883 4064 4275 1865
Size: 2018 181 211 3477
Fragments arranged by size:
3477 2018 211 181
BsrGI T'GTAC_A
Cuts at: 2558 2558
Size: 5887
BssSI C'ACGA_G
Cuts at: 4156 4516 4156
Size: 360 5527
BstAPI GCAn_nnn'nTGC
Cuts at: 620 1508 2460 2638 3262 620
Size: 888 952 178 624 3245
Fragments arranged by size:
3245 952 888 624 178
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5887
BstXI CCAn_nnnn'nTGG
Cuts at: 415 1545 2349 415
Size: 1130 804 3953
Fragments arranged by size:
3953 1130 804
BstYI r'GATC_y
Cuts at: 1 164 506 1442 1762 2058 2910 3377 3400
Size: 163 342 936 320 296 852 467 23
Cuts at: 3400 3735 3981 4995 5081 5093 5439 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
1014 936 852 467 449 346 342 335
320 296 246 163 86 23 12
BtgI C'CryG_G
Cuts at: 125 443 1724 2136 125
Size: 318 1281 412 3876
Fragments arranged by size:
3876 1281 412 318
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3835 4016 4179 3835
Size: 181 163 5543
Fragments arranged by size:
5543 181 163
BtsI GCAGTG_nn'
Cuts at: 938 3128 938
Size: 2190 3697
DraI TTT'AAA
Cuts at: 1491 2693 2884 1491
Size: 1202 191 4494
Fragments arranged by size:
4494 1202 191
DraIII CAC_nnn'GTG
Cuts at: 2128 2128
Size: 5887
DrdI GACnn_nn'nnGTC
Cuts at: 3591 4451 3591
Size: 860 5027
EaeI y'GGCC_r
Cuts at: 1216 2585 3470 3644 4035 4062 5409 1216
Size: 1369 885 174 391 27 1347 1694
Fragments arranged by size:
1694 1369 1347 885 391 174 27
EagI C'GGCC_G
Cuts at: 3470 3470
Size: 5887
EarI CTCTTCn'nnn_
Cuts at: 157 280 1889 2239 2539 3908 4118 157
Size: 123 1609 350 300 1369 210 1926
Fragments arranged by size:
1926 1609 1369 350 300 210 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4093 4405 4551 5697 4093
Size: 312 146 1146 4283
Fragments arranged by size:
4283 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2897 2897
Size: 5887
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1251 1483 1591 1921 2720 3710 4142 4891 5159
Size: 232 108 330 799 990 432 749 268
Cuts at: 5159 1251
Size: 1979
Fragments arranged by size:
1979 990 799 749 432 330 268 232
108
EcoNI CCTnn'n_nnAGG
Cuts at: 2263 2359 2263
Size: 96 5791
EcoO109I rG'GnC_Cy
Cuts at: 210 714 764 1836 2255 3024 210
Size: 504 50 1072 419 769 3073
Fragments arranged by size:
3073 1072 769 504 419 50
EcoRI G'AATT_C
Cuts at: 2773 2889 2773
Size: 116 5771
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 861 1035 3904 861
Size: 174 2869 2844
Fragments arranged by size:
2869 2844 174
FspI TGC'GCA
Cuts at: 3666 3666
Size: 5887
HaeII r_GCGC'y
Cuts at: 1185 1359 3319 3567 4591 5570 1185
Size: 174 1960 248 1024 979 1502
Fragments arranged by size:
1960 1502 1024 979 248 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 726 758 796 828 1432 1464 1639 1671 1969
Size: 32 38 32 604 32 175 32 298
Cuts at: 1969 2001 2050 2071 2082 2086 2103 2118 2774
Size: 32 49 21 11 4 17 15 656
Cuts at: 2774 2790 2806 2822 3727 3757 3759 3789 726
Size: 16 16 16 905 30 2 30 2824
Fragments arranged by size:
2824 905 656 604 298 175 49 38
32 32 32 32 32 30 30 21
17 16 16 16 15 11 4 2
HincII GTy'rAC
Cuts at: 1458 2924 1458
Size: 1466 4421
HindIII A'AGCT_T
Cuts at: 1113 1113
Size: 5887
KasI G'GCGC_C
Cuts at: 1355 3315 3563 1355
Size: 1960 248 3679
Fragments arranged by size:
3679 1960 248
KpnI G_GTAC'C
Cuts at: 2905 2905
Size: 5887
MluI A'CGCG_T
Cuts at: 5320 5320
Size: 5887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 476 2330 4558 4742 476
Size: 1854 2228 184 1621
Fragments arranged by size:
2228 1854 1621 184
MscI TGG'CCA
Cuts at: 1218 3646 1218
Size: 2428 3459
MslI CAynn'nnrTG
Cuts at: 1156 1729 2566 3874 4001 1156
Size: 573 837 1308 127 3042
Fragments arranged by size:
3042 1308 837 573 127
MspA1I CmG'CkG
Cuts at: 520 2996 3310 3670 4685 4930 520
Size: 2476 314 360 1015 245 1477
Fragments arranged by size:
2476 1477 1015 360 314 245
NaeI GCC'GGC
Cuts at: 4066 4066
Size: 5887
NarI GG'CG_CC
Cuts at: 1356 3316 3564 1356
Size: 1960 248 3679
Fragments arranged by size:
3679 1960 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 5887
NgoMIV G'CCGG_C
Cuts at: 4064 4064
Size: 5887
NheI G'CTAG_C
Cuts at: 2182 2182
Size: 5887
NruI TCG'CGA
Cuts at: 4248 4248
Size: 5887
NspI r_CATG'y
Cuts at: 2571 2828 2938 3438 4347 2571
Size: 257 110 500 909 4111
Fragments arranged by size:
4111 909 500 257 110
PciI A'CATG_T
Cuts at: 4343 4343
Size: 5887
PflMI CCAn_nnn'nTGG
Cuts at: 1229 1995 2321 1229
Size: 766 326 4795
Fragments arranged by size:
4795 766 326
PfoI T'CCnGG_A
Cuts at: 2736 2736
Size: 5887
PmeI GTTT'AAAC
Cuts at: 2884 2884
Size: 5887
PmlI CAC'GTG
Cuts at: 1566 1566
Size: 5887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2485 2517 5026 5058 2485
Size: 32 2509 32 3314
Fragments arranged by size:
3314 2509 32 32
PpuMI rG'GwC_Cy
Cuts at: 210 764 1836 2255 210
Size: 554 1072 419 3842
Fragments arranged by size:
3842 1072 554 419
PspOMI G'GGCC_C
Cuts at: 1147 1147
Size: 5887
PstI C_TGCA'G
Cuts at: 2678 2932 3617 2678
Size: 254 685 4948
Fragments arranged by size:
4948 685 254
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5887
PvuII CAG'CTG
Cuts at: 520 3310 3670 520
Size: 2790 360 2737
Fragments arranged by size:
2790 2737 360
RsrII CG'GwC_CG
Cuts at: 4080 4080
Size: 5887
SacI G_AGCT'C
Cuts at: 2899 2899
Size: 5887
SalI G'TCGA_C
Cuts at: 2922 2922
Size: 5887
SanDI GG'GwC_CC
Cuts at: 1836 1836
Size: 5887
SapI GCTCTTCn'nnn_
Cuts at: 280 1889 2539 3908 4118 280
Size: 1609 650 1369 210 2049
Fragments arranged by size:
2049 1609 1369 650 210
SbfI CC_TGCA'GG
Cuts at: 2932 2932
Size: 5887
ScaI AGT'ACT
Cuts at: 2713 5404 2713
Size: 2691 3196
SfcI C'TryA_G
Cuts at: 33 2674 2928 3613 4608 4799 33
Size: 2641 254 685 995 191 1121
Fragments arranged by size:
2641 1121 995 685 254 191
SfoI GGC'GCC
Cuts at: 1357 3317 3565 1357
Size: 1960 248 3679
Fragments arranged by size:
3679 1960 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5887
SmaI CCC'GGG
Cuts at: 2907 2907
Size: 5887
SmlI C'TyrA_G
Cuts at: 2530 3040 3955 4449 4711 2530
Size: 510 915 494 262 3706
Fragments arranged by size:
3706 915 510 494 262
SnaBI TAC'GTA
Cuts at: 2877 2877
Size: 5887
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5887
SphI G_CATG'C
Cuts at: 2828 2938 3438 2828
Size: 110 500 5277
Fragments arranged by size:
5277 500 110
StuI AGG'CCT
Cuts at: 2218 2218
Size: 5887
StyI C'CwwG_G
Cuts at: 125 428 2786 3019 3373 125
Size: 303 2358 233 354 2639
Fragments arranged by size:
2639 2358 354 303 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1567 1894 2593 3468 3601 4060 4271 1567
Size: 327 699 875 133 459 211 3183
Fragments arranged by size:
3183 875 699 459 327 211 133
TatI w'GTAC_w
Cuts at: 1616 2558 2711 5402 1616
Size: 942 153 2691 2101
Fragments arranged by size:
2691 2101 942 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 617 649 2116 2148 2181 2213 5404 5436 617
Size: 32 1467 32 33 32 3191 32 1068
Fragments arranged by size:
3191 1467 1068 33 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3682 3682
Size: 5887
XbaI T'CTAG_A
Cuts at: 2916 2916
Size: 5887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1158 1226 2125 3380 1158
Size: 68 899 1255 3665
Fragments arranged by size:
3665 1255 899 68
XmaI C'CCGG_G
Cuts at: 2905 2905
Size: 5887
XmnI GAAnn'nnTTC
Cuts at: 901 901
Size: 5887
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NruI NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AscI AvrII BcgI BfrBI
BglI BmgBI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HpaI MfeI NdeI
NotI NsiI PacI PasI PshAI PsiI PspXI PsrI
SacII SexAI SfiI SgrAI SrfI SspI SwaI XhoI
ZraI