Restriction map for
pF1KSDA1004
(Circular) (Six-base) MAPSORT of: pF1KSDA1004.seq Check: 4243 from: 1 to: 6625
pF1KSDA1004 6625 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 632 985 3661 632
Size: 353 2676 3596
Fragments arranged by size:
3596 2676 353
Acc65I G'GTAC_C
Cuts at: 172 775 3639 172
Size: 603 2864 3158
Fragments arranged by size:
3158 2864 603
AclI AA'CG_TT
Cuts at: 6465 6465
Size: 6625
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1396 2507 4448 4880 5629 5897 1396
Size: 1111 1941 432 749 268 2124
Fragments arranged by size:
2124 1941 1111 749 432 268
AfeI AGC'GCT
Cuts at: 1141 2392 1141
Size: 1251 5374
AflIII A'CryG_T
Cuts at: 638 1981 3975 5081 6058 638
Size: 1343 1994 1106 977 1205
Fragments arranged by size:
1994 1343 1205 1106 977
AgeI A'CCGG_T
Cuts at: 5013 5013
Size: 6625
AleI CACnn'nnGTG
Cuts at: 1003 2372 1003
Size: 1369 5256
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1039 1039
Size: 6625
AlwNI CAG_nnn'CTG
Cuts at: 1792 2412 3103 3344 3459 3928 5497 1792
Size: 620 691 241 115 469 1569 2920
Fragments arranged by size:
2920 1569 691 620 469 241 115
ApaLI G'TGCA_C
Cuts at: 5395 5395
Size: 6625
ApoI r'AATT_y
Cuts at: 363 1183 1693 3179 3627 5978 6234 363
Size: 820 510 1486 448 2351 256 754
Fragments arranged by size:
2351 1486 820 754 510 448 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6625
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6625
AvaI C'yCGr_G
Cuts at: 562 2321 3643 562
Size: 1759 1322 3544
Fragments arranged by size:
3544 1759 1322
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 767 800 767
Size: 33 6592
BamHI G'GATC_C
Cuts at: 393 1443 3648 393
Size: 1050 2205 3370
Fragments arranged by size:
3370 2205 1050
BanI G'GyrC_C
Cuts at: 172 775 1956 3639 4053 4301 4336 172
Size: 603 1181 1683 414 248 35 2461
Fragments arranged by size:
2461 1683 1181 603 414 248 35
BanII G_rGCy'C
Cuts at: 935 1423 2555 2781 3422 3637 4667 935
Size: 488 1132 226 641 215 1030 2893
Fragments arranged by size:
2893 1132 1030 641 488 226 215
BbeI G_GCGC'C
Cuts at: 4057 4305 4057
Size: 248 6377
BbsI GAAGACnn'nnnn_
Cuts at: 807 1357 2820 3386 807
Size: 550 1463 566 4046
Fragments arranged by size:
4046 1463 566 550
BbvCI CC'TCA_GC
Cuts at: 1201 2839 3109 1201
Size: 1638 270 4717
Fragments arranged by size:
4717 1638 270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3505 3515 3539 3549 3505
Size: 10 24 10 6581
Fragments arranged by size:
6581 24 10 10
BciVI GTATCCnnnnn_n'
Cuts at: 4521 5284 6625 4521
Size: 763 1341 4521
Fragments arranged by size:
4521 1341 763
BclI T'GATC_A
Cuts at: 4143 4143
Size: 6625
BglI GCCn_nnn'nGGC
Cuts at: 2593 2593
Size: 6625
BglII A'GATC_T
Cuts at: 4138 6177 4138
Size: 2039 4586
BlpI GC'TnA_GC
Cuts at: 3735 3735
Size: 6625
Bme1580I G_kGCm'C
Cuts at: 2576 2954 4248 4341 5399 2576
Size: 378 1294 93 1058 3802
Fragments arranged by size:
3802 1294 1058 378 93
BmgBI CAC'GTC
Cuts at: 462 2882 462
Size: 2420 4205
BmrI ACTGGGnnnn_n'
Cuts at: 636 1354 1443 3835 3916 4251 6397 636
Size: 718 89 2392 81 335 2146 864
Fragments arranged by size:
2392 2146 864 718 335 89 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 365 559 2181 4990 365
Size: 194 1622 2809 2000
Fragments arranged by size:
2809 2000 1622 194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 868 1633 1673 2071 3102 604
Size: 264 765 40 398 1031 4127
Fragments arranged by size:
4127 1031 765 398 264 40
Bpu10I CC'TnA_GC
Cuts at: 1201 2780 2839 3109 1201
Size: 1579 59 270 4717
Fragments arranged by size:
4717 1579 270 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 360 919 1299 2949 3799 4678 5172 5470 360
Size: 559 380 1650 850 879 494 298 1515
Fragments arranged by size:
1650 1515 879 850 559 494 380 298
BsaI GGTCTCn'nnnn_
Cuts at: 511 6329 511
Size: 5818 807
BsaAI yAC'GTr
Cuts at: 3615 3976 3615
Size: 361 6264
BsaBI GATnn'nnATC
Cuts at: 6 4137 6176 6
Size: 4131 2039 455
Fragments arranged by size:
4131 2039 455
BsaHI Gr'CG_yC
Cuts at: 4054 4302 5006 6502 4054
Size: 248 704 1496 4177
Fragments arranged by size:
4177 1496 704 248
BsaWI w'CCGG_w
Cuts at: 3239 3812 4333 5013 5287 5434 5845 3239
Size: 573 521 680 274 147 411 4019
Fragments arranged by size:
4019 680 573 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 685 715 685
Size: 30 6595
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 541 544 1630 2589 2689 2707 2710 2719 2722
Size: 3 1086 959 100 18 3 9 3
Cuts at: 2722 2725 2728 2731 2752 3061 541
Size: 3 3 3 21 309 4105
Fragments arranged by size:
4105 1086 959 309 100 21 18 9
3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 1390 2132 2359 2464 2646 2660 2682 4048 4806
Size: 742 227 105 182 14 22 1366 758
Cuts at: 4806 5385 5881 1390
Size: 579 496 2134
Fragments arranged by size:
2134 1366 758 742 579 496 227 182
105 22 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 616 733 1528 2656 2753 2768 241
Size: 375 117 795 1128 97 15 4098
Fragments arranged by size:
4098 1128 795 375 117 97 15
BsiEI CG_ry'CG
Cuts at: 104 4165 4211 5421 104
Size: 4061 46 1210 1308
Fragments arranged by size:
4061 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 935 1458 2555 3422 3542 3637 4415 4605 5399
Size: 523 1097 867 120 95 778 190 794
Cuts at: 5399 935
Size: 2161
Fragments arranged by size:
2161 1097 867 794 778 523 190 120
95
BsmI GAATG_Cn'
Cuts at: 2053 2053
Size: 6625
BsmBI CGTCTCn'nnnn_
Cuts at: 1818 2783 1818
Size: 965 5660
Bsp1286I G_dGCh'C
Cuts at: 935 1423 1458 2555 2576 2781 2954 3422 3542
Size: 488 35 1097 21 205 173 468 120
Cuts at: 3542 3637 4248 4341 4415 4605 4667 5399 935
Size: 95 611 93 74 190 62 732 2161
Fragments arranged by size:
2161 1097 732 611 488 468 205 190
173 120 95 93 74 62 35 21
BspEI T'CCGG_A
Cuts at: 3239 5845 3239
Size: 2606 4019
BspHI T'CATG_A
Cuts at: 5801 5801
Size: 6625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1363 3421 3673 3887 4189 4570 1363
Size: 2058 252 214 302 381 3418
Fragments arranged by size:
3418 2058 381 302 252 214
BsrBI CCG'CTC
Cuts at: 50 4915 4969 6455 6620 50
Size: 4865 54 1486 165 55
Fragments arranged by size:
4865 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1589 2067 3476 4535 1589
Size: 478 1409 1059 3679
Fragments arranged by size:
3679 1409 1059 478
BsrFI r'CCGG_y
Cuts at: 3201 4621 4802 5013 3201
Size: 1420 181 211 4813
Fragments arranged by size:
4813 1420 211 181
BssSI C'ACGA_G
Cuts at: 4894 5254 4894
Size: 360 6265
BstAPI GCAn_nnn'nTGC
Cuts at: 1355 2412 3103 4000 1355
Size: 1057 691 897 3980
Fragments arranged by size:
3980 1057 897 691
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6625
BstEII G'GTnAC_C
Cuts at: 901 901
Size: 6625
BstXI CCAn_nnnn'nTGG
Cuts at: 2132 2132
Size: 6625
BstYI r'GATC_y
Cuts at: 1 345 393 1443 2504 3648 4115 4138 4473
Size: 344 48 1050 1061 1144 467 23 335
Cuts at: 4473 4719 5733 5819 5831 6177 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1144 1061 1050 1014 467 449 346 344
335 246 86 48 23 12
BstZ17I GTA'TAC
Cuts at: 986 986
Size: 6625
Bsu36I CC'TnA_GG
Cuts at: 963 963
Size: 6625
BtgI C'CryG_G
Cuts at: 125 491 608 1765 2438 2556 125
Size: 366 117 1157 673 118 4194
Fragments arranged by size:
4194 1157 673 366 118 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1848 2264 2399 4573 4754 4917 1848
Size: 416 135 2174 181 163 3556
Fragments arranged by size:
3556 2174 416 181 163 135
BtsI GCAGTG_nn'
Cuts at: 1262 1540 2640 3342 3380 3866 1262
Size: 278 1100 702 38 486 4021
Fragments arranged by size:
4021 1100 702 486 278 38
DraI TTT'AAA
Cuts at: 3622 3622
Size: 6625
DrdI GACnn_nn'nnGTC
Cuts at: 4329 5189 4329
Size: 860 5765
EaeI y'GGCC_r
Cuts at: 643 1672 1705 2763 4208 4382 4773 4800 6147
Size: 1029 33 1058 1445 174 391 27 1347
Cuts at: 6147 643
Size: 1121
Fragments arranged by size:
1445 1347 1121 1058 1029 391 174 33
27
EagI C'GGCC_G
Cuts at: 4208 4208
Size: 6625
EarI CTCTTCn'nnn_
Cuts at: 242 2009 2189 2696 4646 4856 242
Size: 1767 180 507 1950 210 2011
Fragments arranged by size:
2011 1950 1767 507 210 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4831 5143 5289 6435 4831
Size: 312 146 1146 5021
Fragments arranged by size:
5021 1146 312 146
EcoICRI GAG'CTC
Cuts at: 933 2553 3420 3635 933
Size: 1620 867 215 3923
Fragments arranged by size:
3923 1620 867 215
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 868 1396 1633 1673 2071 2507 3102 4448
Size: 264 528 237 40 398 436 595 1346
Cuts at: 4448 4880 5629 5897 604
Size: 432 749 268 1332
Fragments arranged by size:
1346 1332 749 595 528 436 432 398
268 264 237 40
EcoNI CCTnn'n_nnAGG
Cuts at: 1177 1177
Size: 6625
EcoO109I rG'GnC_Cy
Cuts at: 602 1912 2958 3137 3171 3762 602
Size: 1310 1046 179 34 591 3465
Fragments arranged by size:
3465 1310 1046 591 179 34
EcoRI G'AATT_C
Cuts at: 363 3627 363
Size: 3264 3361
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4642 4642
Size: 6625
FspI TGC'GCA
Cuts at: 4404 4404
Size: 6625
HaeII r_GCGC'y
Cuts at: 1143 2394 4057 4305 5329 6308 1143
Size: 1251 1663 248 1024 979 1460
Fragments arranged by size:
1663 1460 1251 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 480 512 895 927 2058 2090 2504 2536 3129
Size: 32 383 32 1131 32 414 32 593
Cuts at: 3129 3161 3320 3352 3384 4465 4495 4497 4527
Size: 32 159 32 32 1081 30 2 30
Cuts at: 4527 480
Size: 2578
Fragments arranged by size:
2578 1131 1081 593 414 383 159 32
32 32 32 32 32 32 30 30
2
HincII GTy'rAC
Cuts at: 1326 1772 3662 1326
Size: 446 1890 4289
Fragments arranged by size:
4289 1890 446
HindIII A'AGCT_T
Cuts at: 1687 1687
Size: 6625
HpaI GTT'AAC
Cuts at: 1772 1772
Size: 6625
KasI G'GCGC_C
Cuts at: 4053 4301 4053
Size: 248 6377
KpnI G_GTAC'C
Cuts at: 176 779 3643 176
Size: 603 2864 3158
Fragments arranged by size:
3158 2864 603
MluI A'CGCG_T
Cuts at: 6058 6058
Size: 6625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1115 1208 1508 1968 2986 5296 5480 1115
Size: 93 300 460 1018 2310 184 2260
Fragments arranged by size:
2310 2260 1018 460 300 184 93
MscI TGG'CCA
Cuts at: 645 1674 4384 645
Size: 1029 2710 2886
Fragments arranged by size:
2886 2710 1029
MslI CAynn'nnrTG
Cuts at: 460 484 1003 1042 2287 2372 4612 4739 460
Size: 24 519 39 1245 85 2240 127 2346
Fragments arranged by size:
2346 2240 1245 519 127 85 39 24
MspA1I CmG'CkG
Cuts at: 330 1789 2468 2808 3122 3734 4048 4408 5423
Size: 1459 679 340 314 612 314 360 1015
Cuts at: 5423 5668 330
Size: 245 1287
Fragments arranged by size:
1459 1287 1015 679 612 360 340 314
314 245
NaeI GCC'GGC
Cuts at: 4804 4804
Size: 6625
NarI GG'CG_CC
Cuts at: 4054 4302 4054
Size: 248 6377
NcoI C'CATG_G
Cuts at: 125 491 2438 125
Size: 366 1947 4312
Fragments arranged by size:
4312 1947 366
NgoMIV G'CCGG_C
Cuts at: 4802 4802
Size: 6625
NruI TCG'CGA
Cuts at: 4986 4986
Size: 6625
NspI r_CATG'y
Cuts at: 642 1985 3315 3676 4176 5085 642
Size: 1343 1330 361 500 909 2182
Fragments arranged by size:
2182 1343 1330 909 500 361
PasI CC'CwG_GG
Cuts at: 2347 2347
Size: 6625
PciI A'CATG_T
Cuts at: 638 1981 5081 638
Size: 1343 3100 2182
Fragments arranged by size:
3100 2182 1343
PflMI CCAn_nnn'nTGG
Cuts at: 846 846
Size: 6625
PmeI GTTT'AAAC
Cuts at: 3622 3622
Size: 6625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5764 5796 5764
Size: 32 6593
PpuMI rG'GwC_Cy
Cuts at: 3137 3171 3137
Size: 34 6591
PshAI GACnn'nnGTC
Cuts at: 3338 3338
Size: 6625
PspXI vC'TCGA_Gb
Cuts at: 562 562
Size: 6625
PstI C_TGCA'G
Cuts at: 1891 2297 2423 2585 3099 3349 3670 4355 1891
Size: 406 126 162 514 250 321 685 4161
Fragments arranged by size:
4161 685 514 406 321 250 162 126
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6625
PvuII CAG'CTG
Cuts at: 1789 3122 4048 4408 1789
Size: 1333 926 360 4006
Fragments arranged by size:
4006 1333 926 360
RsrII CG'GwC_CG
Cuts at: 4818 4818
Size: 6625
SacI G_AGCT'C
Cuts at: 935 2555 3422 3637 935
Size: 1620 867 215 3923
Fragments arranged by size:
3923 1620 867 215
SalI G'TCGA_C
Cuts at: 3660 3660
Size: 6625
SapI GCTCTTCn'nnn_
Cuts at: 4646 4856 4646
Size: 210 6415
SbfI CC_TGCA'GG
Cuts at: 3670 3670
Size: 6625
ScaI AGT'ACT
Cuts at: 721 6142 721
Size: 5421 1204
SexAI A'CCwGG_T
Cuts at: 3213 3213
Size: 6625
SfcI C'TryA_G
Cuts at: 33 833 992 1310 1410 1661 1887 2293 2419
Size: 800 159 318 100 251 226 406 126
Cuts at: 2419 2533 2581 3095 3345 3666 4351 5346 5537
Size: 114 48 514 250 321 685 995 191
Cuts at: 5537 33
Size: 1121
Fragments arranged by size:
1121 995 800 685 514 406 321 318
251 250 226 191 159 126 114 100
48
SfiI GGCCn_nnn'nGGCC
Cuts at: 2593 2593
Size: 6625
SfoI GGC'GCC
Cuts at: 4055 4303 4055
Size: 248 6377
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6625
SmaI CCC'GGG
Cuts at: 2323 3645 2323
Size: 1322 5303
SmlI C'TyrA_G
Cuts at: 339 562 934 1278 2928 3778 4693 5187 5449
Size: 223 372 344 1650 850 915 494 262
Cuts at: 5449 339
Size: 1515
Fragments arranged by size:
1650 1515 915 850 494 372 344 262
223
SnaBI TAC'GTA
Cuts at: 3615 3615
Size: 6625
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6625
SphI G_CATG'C
Cuts at: 3676 4176 3676
Size: 500 6125
StuI AGG'CCT
Cuts at: 1505 3269 1505
Size: 1764 4861
StyI C'CwwG_G
Cuts at: 125 491 646 1178 2438 3757 4111 125
Size: 366 155 532 1260 1319 354 2639
Fragments arranged by size:
2639 1319 1260 532 366 354 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 889 1974 2295 2327 3265 4206 4339 4798 5009
Size: 1085 321 32 938 941 133 459 211
Cuts at: 5009 889
Size: 2505
Fragments arranged by size:
2505 1085 941 938 459 321 211 133
32
TatI w'GTAC_w
Cuts at: 719 1148 1665 6140 719
Size: 429 517 4475 1204
Fragments arranged by size:
4475 1204 517 429
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1435 1467 1641 1673 2548 2580 6142 6174 1435
Size: 32 174 32 875 32 3562 32 1886
Fragments arranged by size:
3562 1886 875 174 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2337 4420 2337
Size: 2083 4542
XbaI T'CTAG_A
Cuts at: 3654 3654
Size: 6625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 166 766 2962 4118 166
Size: 600 2196 1156 2673
Fragments arranged by size:
2673 2196 1156 600
XhoI C'TCGA_G
Cuts at: 562 562
Size: 6625
XmaI C'CCGG_G
Cuts at: 2321 3643 2321
Size: 1322 5303
XmnI GAAnn'nnTTC
Cuts at: 146 1505 146
Size: 1359 5266
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI ApaI AscI AvrII
BfrBI BmtI BsiWI BsrGI BssHII ClaI CspCI DraIII
EcoRV FseI FspAI MfeI NdeI NheI NotI NsiI
PacI PfoI PmlI PsiI PspOMI PsrI SacII SanDI
SgrAI SrfI SspI SwaI ZraI