Restriction map for
pF1KSDA0997
(Circular) (Six-base) MAPSORT of: pF1KSDA0997.seq Check: 6599 from: 1 to: 5071
pF1KSDA0997 5071 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 2107 2107
Size: 5071
Acc65I G'GTAC_C
Cuts at: 2085 2085
Size: 5071
AclI AA'CG_TT
Cuts at: 4911 4911
Size: 5071
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 1241 1498 2894 3326 4075 4343 142
Size: 1099 257 1396 432 749 268 870
Fragments arranged by size:
1396 1099 870 749 432 268 257
AflII C'TTAA_G
Cuts at: 1902 1902
Size: 5071
AflIII A'CryG_T
Cuts at: 744 1954 2421 3527 4504 744
Size: 1210 467 1106 977 1311
Fragments arranged by size:
1311 1210 1106 977 467
AgeI A'CCGG_T
Cuts at: 3459 3459
Size: 5071
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 320 320
Size: 5071
AlwNI CAG_nnn'CTG
Cuts at: 2374 3943 2374
Size: 1569 3502
ApaLI G'TGCA_C
Cuts at: 3841 3841
Size: 5071
ApoI r'AATT_y
Cuts at: 925 2073 4424 4680 925
Size: 1148 2351 256 1316
Fragments arranged by size:
2351 1316 1148 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5071
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5071
AvaI C'yCGr_G
Cuts at: 2089 2089
Size: 5071
BamHI G'GATC_C
Cuts at: 2094 2094
Size: 5071
BanI G'GyrC_C
Cuts at: 2085 2499 2747 2782 2085
Size: 414 248 35 4374
Fragments arranged by size:
4374 414 248 35
BanII G_rGCy'C
Cuts at: 1544 2083 3113 1544
Size: 539 1030 3502
Fragments arranged by size:
3502 1030 539
BbeI G_GCGC'C
Cuts at: 2503 2751 2503
Size: 248 4823
BbsI GAAGACnn'nnnn_
Cuts at: 473 473
Size: 5071
BciVI GTATCCnnnnn_n'
Cuts at: 1696 2967 3730 5071 1696
Size: 1271 763 1341 1696
Fragments arranged by size:
1696 1341 1271 763
BclI T'GATC_A
Cuts at: 2589 2589
Size: 5071
BfrBI ATG'CAT
Cuts at: 1387 1387
Size: 5071
BglII A'GATC_T
Cuts at: 868 1548 2584 4623 868
Size: 680 1036 2039 1316
Fragments arranged by size:
2039 1316 1036 680
BlpI GC'TnA_GC
Cuts at: 2181 2181
Size: 5071
Bme1580I G_kGCm'C
Cuts at: 2694 2787 3845 2694
Size: 93 1058 3920
Fragments arranged by size:
3920 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 2281 2362 2697 4843 2281
Size: 81 335 2146 2509
Fragments arranged by size:
2509 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3436 3436
Size: 5071
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 384 384
Size: 5071
Bpu10I CC'TnA_GC
Cuts at: 683 683
Size: 5071
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2245 3124 3618 3916 2245
Size: 879 494 298 3400
Fragments arranged by size:
3400 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4775 4775
Size: 5071
BsaAI yAC'GTr
Cuts at: 2061 2422 2061
Size: 361 4710
BsaBI GATnn'nnATC
Cuts at: 6 2583 4622 6
Size: 2577 2039 455
Fragments arranged by size:
2577 2039 455
BsaHI Gr'CG_yC
Cuts at: 2500 2748 3452 4948 2500
Size: 248 704 1496 2623
Fragments arranged by size:
2623 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2258 2779 3459 3733 3880 4291 2258
Size: 521 680 274 147 411 3038
Fragments arranged by size:
3038 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1414 1444 1414
Size: 30 5041
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 995 1435 1594 995
Size: 440 159 4472
Fragments arranged by size:
4472 440 159
BseYI C'CCAG_C
Cuts at: 136 2494 3252 3831 4327 136
Size: 2358 758 579 496 880
Fragments arranged by size:
2358 880 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 359 626 944 1978 290
Size: 69 267 318 1034 3383
Fragments arranged by size:
3383 1034 318 267 69
BsiEI CG_ry'CG
Cuts at: 104 2611 2657 3867 104
Size: 2507 46 1210 1308
Fragments arranged by size:
2507 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 2083 2861 3051 3845 2083
Size: 778 190 794 3309
Fragments arranged by size:
3309 794 778 190
Bsp1286I G_dGCh'C
Cuts at: 1544 2083 2694 2787 2861 3051 3113 3845 1544
Size: 539 611 93 74 190 62 732 2770
Fragments arranged by size:
2770 732 611 539 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4291 4291
Size: 5071
BspHI T'CATG_A
Cuts at: 284 4247 284
Size: 3963 1108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2119 2333 2635 3016 2119
Size: 214 302 381 4174
Fragments arranged by size:
4174 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3361 3415 4901 5066 50
Size: 3311 54 1486 165 55
Fragments arranged by size:
3311 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 431 591 2981 431
Size: 160 2390 2521
Fragments arranged by size:
2521 2390 160
BsrFI r'CCGG_y
Cuts at: 3067 3248 3459 3067
Size: 181 211 4679
Fragments arranged by size:
4679 211 181
BssSI C'ACGA_G
Cuts at: 3340 3700 3340
Size: 360 4711
BstAPI GCAn_nnn'nTGC
Cuts at: 2446 2446
Size: 5071
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5071
BstYI r'GATC_y
Cuts at: 1 868 1548 2094 2561 2584 2919 3165 4179
Size: 867 680 546 467 23 335 246 1014
Cuts at: 4179 4265 4277 4623 1
Size: 86 12 346 449
Fragments arranged by size:
1014 867 680 546 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 5071
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3019 3200 3363 3019
Size: 181 163 4727
Fragments arranged by size:
4727 181 163
BtsI GCAGTG_nn'
Cuts at: 2312 2312
Size: 5071
DraI TTT'AAA
Cuts at: 417 1707 2068 417
Size: 1290 361 3420
Fragments arranged by size:
3420 1290 361
DrdI GACnn_nn'nnGTC
Cuts at: 2775 3635 2775
Size: 860 4211
EaeI y'GGCC_r
Cuts at: 1743 2018 2654 2828 3219 3246 4593 1743
Size: 275 636 174 391 27 1347 2221
Fragments arranged by size:
2221 1347 636 391 275 174 27
EagI C'GGCC_G
Cuts at: 2654 2654
Size: 5071
EarI CTCTTCn'nnn_
Cuts at: 513 650 1061 1217 1592 3092 3302 513
Size: 137 411 156 375 1500 210 2282
Fragments arranged by size:
2282 1500 411 375 210 156 137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 780 3277 3589 3735 4881 780
Size: 2497 312 146 1146 970
Fragments arranged by size:
2497 1146 970 312 146
EcoICRI GAG'CTC
Cuts at: 2081 2081
Size: 5071
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 384 1241 1498 2894 3326 4075 4343 142
Size: 242 857 257 1396 432 749 268 870
Fragments arranged by size:
1396 870 857 749 432 268 257 242
EcoO109I rG'GnC_Cy
Cuts at: 1343 1425 2035 2208 1343
Size: 82 610 173 4206
Fragments arranged by size:
4206 610 173 82
EcoRI G'AATT_C
Cuts at: 2073 2073
Size: 5071
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3088 3088
Size: 5071
FspI TGC'GCA
Cuts at: 2850 2850
Size: 5071
HaeII r_GCGC'y
Cuts at: 2503 2751 3775 4754 2503
Size: 248 1024 979 2820
Fragments arranged by size:
2820 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 643 675 1414 1417 1446 1449 1600 1632 2911
Size: 32 739 3 29 3 151 32 1279
Cuts at: 2911 2941 2943 2973 643
Size: 30 2 30 2741
Fragments arranged by size:
2741 1279 739 151 32 32 30 30
29 3 3 2
HincII GTy'rAC
Cuts at: 2108 2108
Size: 5071
KasI G'GCGC_C
Cuts at: 2499 2747 2499
Size: 248 4823
KpnI G_GTAC'C
Cuts at: 2089 2089
Size: 5071
MfeI C'AATT_G
Cuts at: 212 212
Size: 5071
MluI A'CGCG_T
Cuts at: 4504 4504
Size: 5071
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3742 3926 3742
Size: 184 4887
MscI TGG'CCA
Cuts at: 1745 2020 2830 1745
Size: 275 810 3986
Fragments arranged by size:
3986 810 275
MslI CAynn'nnrTG
Cuts at: 3058 3185 3058
Size: 127 4944
MspA1I CmG'CkG
Cuts at: 1893 2180 2494 2854 3869 4114 1893
Size: 287 314 360 1015 245 2850
Fragments arranged by size:
2850 1015 360 314 287 245
NaeI GCC'GGC
Cuts at: 3250 3250
Size: 5071
NarI GG'CG_CC
Cuts at: 2500 2748 2500
Size: 248 4823
NcoI C'CATG_G
Cuts at: 125 125
Size: 5071
NdeI CA'TA_TG
Cuts at: 1781 1781
Size: 5071
NgoMIV G'CCGG_C
Cuts at: 3248 3248
Size: 5071
NruI TCG'CGA
Cuts at: 3432 3432
Size: 5071
NsiI A_TGCA'T
Cuts at: 1389 1389
Size: 5071
NspI r_CATG'y
Cuts at: 829 1475 1525 1958 2122 2622 3531 829
Size: 646 50 433 164 500 909 2369
Fragments arranged by size:
2369 909 646 500 433 164 50
PciI A'CATG_T
Cuts at: 1954 3527 1954
Size: 1573 3498
PflMI CCAn_nnn'nTGG
Cuts at: 599 599
Size: 5071
PfoI T'CCnGG_A
Cuts at: 473 473
Size: 5071
PmeI GTTT'AAAC
Cuts at: 2068 2068
Size: 5071
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4210 4242 4210
Size: 32 5039
PpuMI rG'GwC_Cy
Cuts at: 1425 1425
Size: 5071
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 696 728 696
Size: 32 5039
PstI C_TGCA'G
Cuts at: 2116 2801 2116
Size: 685 4386
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5071
PvuII CAG'CTG
Cuts at: 2494 2854 2494
Size: 360 4711
RsrII CG'GwC_CG
Cuts at: 3264 3264
Size: 5071
SacI G_AGCT'C
Cuts at: 2083 2083
Size: 5071
SalI G'TCGA_C
Cuts at: 2106 2106
Size: 5071
SapI GCTCTTCn'nnn_
Cuts at: 1217 3092 3302 1217
Size: 1875 210 2986
Fragments arranged by size:
2986 1875 210
SbfI CC_TGCA'GG
Cuts at: 2116 2116
Size: 5071
ScaI AGT'ACT
Cuts at: 303 4588 303
Size: 4285 786
SfcI C'TryA_G
Cuts at: 33 258 436 1130 2112 2797 3792 3983 33
Size: 225 178 694 982 685 995 191 1121
Fragments arranged by size:
1121 995 982 694 685 225 191 178
SfoI GGC'GCC
Cuts at: 2501 2749 2501
Size: 248 4823
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5071
SmaI CCC'GGG
Cuts at: 2091 2091
Size: 5071
SmlI C'TyrA_G
Cuts at: 1902 2224 3139 3633 3895 1902
Size: 322 915 494 262 3078
Fragments arranged by size:
3078 915 494 322 262
SnaBI TAC'GTA
Cuts at: 2061 2061
Size: 5071
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5071
SphI G_CATG'C
Cuts at: 2122 2622 2122
Size: 500 4571
StyI C'CwwG_G
Cuts at: 125 2203 2557 125
Size: 2078 354 2639
Fragments arranged by size:
2639 2078 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2652 2785 3244 3455 2652
Size: 133 459 211 4268
Fragments arranged by size:
4268 459 211 133
TatI w'GTAC_w
Cuts at: 301 838 1365 4586 301
Size: 537 527 3221 786
Fragments arranged by size:
3221 786 537 527
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4588 4620 4588
Size: 32 5039
Tth111I GACn'n_nGTC
Cuts at: 2866 2866
Size: 5071
XbaI T'CTAG_A
Cuts at: 2100 2100
Size: 5071
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2564 2564
Size: 5071
XmaI C'CCGG_G
Cuts at: 2089 2089
Size: 5071
XmnI GAAnn'nnTTC
Cuts at: 1124 1124
Size: 5071
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NruI NsiI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI NheI NotI PacI PasI PmlI PshAI PsiI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SrfI
SspI StuI SwaI XhoI ZraI