Restriction map for
pF1KSDA0979
(Circular) (Six-base) MAPSORT of: pF1KSDA0979.seq Check: 2217 from: 1 to: 7478
pF1KSDA0979 7478 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 659 4516 659
Size: 3857 3621
Acc65I G'GTAC_C
Cuts at: 735 4494 735
Size: 3759 3719
AclI AA'CG_TT
Cuts at: 7318 7318
Size: 7478
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 302 425 811 1874 3064 3878 5303 5735
Size: 9 123 386 1063 1190 814 1425 432
Cuts at: 5735 6482 6750 293
Size: 747 268 1021
Fragments arranged by size:
1425 1190 1063 1021 814 747 432 386
268 123 9
AflII C'TTAA_G
Cuts at: 2083 2676 2083
Size: 593 6885
AflIII A'CryG_T
Cuts at: 4830 5934 6911 4830
Size: 1104 977 5397
Fragments arranged by size:
5397 1104 977
AgeI A'CCGG_T
Cuts at: 5866 5866
Size: 7478
AleI CACnn'nnGTG
Cuts at: 1777 1777
Size: 7478
AlwNI CAG_nnn'CTG
Cuts at: 846 1399 2185 3901 4783 6350 846
Size: 553 786 1716 882 1567 1974
Fragments arranged by size:
1974 1716 1567 882 786 553
ApaLI G'TGCA_C
Cuts at: 1957 6248 1957
Size: 4291 3187
ApoI r'AATT_y
Cuts at: 581 750 958 1129 1367 1728 1768 2195 2600
Size: 169 208 171 238 361 40 427 405
Cuts at: 2600 3435 4482 6831 7087 581
Size: 835 1047 2349 256 972
Fragments arranged by size:
2349 1047 972 835 427 405 361 256
238 208 171 169 40
AseI AT'TA_AT
Cuts at: 20 3208 20
Size: 3188 4290
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7478
AvaI C'yCGr_G
Cuts at: 4498 4498
Size: 7478
BamHI G'GATC_C
Cuts at: 4503 4503
Size: 7478
BanI G'GyrC_C
Cuts at: 735 4494 4908 5156 5191 735
Size: 3759 414 248 35 3022
Fragments arranged by size:
3759 3022 414 248 35
BanII G_rGCy'C
Cuts at: 493 673 911 2994 4492 5522 493
Size: 180 238 2083 1498 1030 2449
Fragments arranged by size:
2449 2083 1498 1030 238 180
BbeI G_GCGC'C
Cuts at: 4912 5160 4912
Size: 248 7230
BbsI GAAGACnn'nnnn_
Cuts at: 3686 4260 3686
Size: 574 6904
BciVI GTATCCnnnnn_n'
Cuts at: 474 708 2332 5376 6137 7478 474
Size: 234 1624 3044 761 1341 474
Fragments arranged by size:
3044 1624 1341 761 474 234
BclI T'GATC_A
Cuts at: 1284 1925 2052 2460 4998 1284
Size: 641 127 408 2538 3764
Fragments arranged by size:
3764 2538 641 408 127
BglI GCCn_nnn'nGGC
Cuts at: 1068 1068
Size: 7478
BglII A'GATC_T
Cuts at: 2511 4993 7030 2511
Size: 2482 2037 2959
Fragments arranged by size:
2959 2482 2037
BlpI GC'TnA_GC
Cuts at: 4590 4590
Size: 7478
Bme1580I G_kGCm'C
Cuts at: 1961 2958 5103 5196 6252 1961
Size: 997 2145 93 1056 3187
Fragments arranged by size:
3187 2145 1056 997 93
BmgBI CAC'GTC
Cuts at: 2755 2755
Size: 7478
BmrI ACTGGGnnnn_n'
Cuts at: 2346 4690 4771 5106 7250 2346
Size: 2344 81 335 2144 2574
Fragments arranged by size:
2574 2344 2144 335 81
BmtI G_CTAG'C
Cuts at: 1139 1139
Size: 7478
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 818 3008 5845 818
Size: 2190 2837 2451
Fragments arranged by size:
2837 2451 2190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4073 4073
Size: 7478
Bpu10I CC'TnA_GC
Cuts at: 1751 1751
Size: 7478
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 541 788 2956 4654 5533 6025 6323 316
Size: 225 247 2168 1698 879 492 298 1471
Fragments arranged by size:
2168 1698 1471 879 492 298 247 225
BsaI GGTCTCn'nnnn_
Cuts at: 2341 7182 2341
Size: 4841 2637
BsaAI yAC'GTr
Cuts at: 4470 4831 4470
Size: 361 7117
BsaBI GATnn'nnATC
Cuts at: 6 4992 7029 6
Size: 4986 2037 455
Fragments arranged by size:
4986 2037 455
BsaHI Gr'CG_yC
Cuts at: 4909 5157 5859 7355 4909
Size: 248 702 1496 5032
Fragments arranged by size:
5032 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4667 5188 5866 6140 6287 6698 4667
Size: 521 678 274 147 411 5447
Fragments arranged by size:
5447 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 1000 3817 4114 4117 4423 4426 226
Size: 774 2817 297 3 306 3 3278
Fragments arranged by size:
3278 2817 774 306 297 3 3
BseYI C'CCAG_C
Cuts at: 590 950 2770 4903 5661 6238 6734 590
Size: 360 1820 2133 758 577 496 1334
Fragments arranged by size:
2133 1820 1334 758 577 496 360
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2171 2171
Size: 7478
BsiEI CG_ry'CG
Cuts at: 104 5020 5066 6274 104
Size: 4916 46 1208 1308
Fragments arranged by size:
4916 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 673 911 1961 1986 2994 4182 4492 5270 5460
Size: 238 1050 25 1008 1188 310 778 190
Cuts at: 5460 6252 673
Size: 792 1899
Fragments arranged by size:
1899 1188 1050 1008 792 778 310 238
190 25
BsmI GAATG_Cn'
Cuts at: 2872 2872
Size: 7478
BsmBI CGTCTCn'nnnn_
Cuts at: 216 3722 216
Size: 3506 3972
Bsp1286I G_dGCh'C
Cuts at: 493 673 911 1961 1986 2958 2994 4182 4492
Size: 180 238 1050 25 972 36 1188 310
Cuts at: 4492 5103 5196 5270 5460 5522 6252 493
Size: 611 93 74 190 62 730 1719
Fragments arranged by size:
1719 1188 1050 972 730 611 310 238
190 180 93 74 62 36 25
BspEI T'CCGG_A
Cuts at: 6698 6698
Size: 7478
BspHI T'CATG_A
Cuts at: 1148 1760 2519 6654 1148
Size: 612 759 4135 1972
Fragments arranged by size:
4135 1972 759 612
BspMI ACCTGCnnnn'nnnn_
Cuts at: 747 1076 2059 4528 4742 5044 5425 747
Size: 329 983 2469 214 302 381 2800
Fragments arranged by size:
2800 2469 983 381 329 302 214
BsrBI CCG'CTC
Cuts at: 50 5770 5824 7308 7473 50
Size: 5720 54 1484 165 55
Fragments arranged by size:
5720 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 527 582 981 3010 3600 5390 527
Size: 55 399 2029 590 1790 2615
Fragments arranged by size:
2615 2029 1790 590 399 55
BsrFI r'CCGG_y
Cuts at: 5476 5657 5866 5476
Size: 181 209 7088
Fragments arranged by size:
7088 209 181
BsrGI T'GTAC_A
Cuts at: 3320 3320
Size: 7478
BssSI C'ACGA_G
Cuts at: 5749 6107 5749
Size: 358 7120
BstAPI GCAn_nnn'nTGC
Cuts at: 2185 2953 4855 2185
Size: 768 1902 4808
Fragments arranged by size:
4808 1902 768
BstBI TT'CG_AA
Cuts at: 110 5839 110
Size: 5729 1749
BstYI r'GATC_y
Cuts at: 1 1496 2391 2511 2970 4503 4970 4993 5328
Size: 1495 895 120 459 1533 467 23 335
Cuts at: 5328 5574 6586 6672 6684 7030 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1533 1495 1012 895 467 459 449 346
335 246 120 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1211 3920 1211
Size: 2709 4769
BtgI C'CryG_G
Cuts at: 125 125
Size: 7478
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5428 5609 5772 5428
Size: 181 163 7134
Fragments arranged by size:
7134 181 163
BtsI GCAGTG_nn'
Cuts at: 1078 3919 4721 1078
Size: 2841 802 3835
Fragments arranged by size:
3835 2841 802
DraI TTT'AAA
Cuts at: 296 755 963 1583 4477 296
Size: 459 208 620 2894 3297
Fragments arranged by size:
3297 2894 620 459 208
DraIII CAC_nnn'GTG
Cuts at: 3329 4167 3329
Size: 838 6640
DrdI GACnn_nn'nnGTC
Cuts at: 2294 5184 6042 2294
Size: 2890 858 3730
Fragments arranged by size:
3730 2890 858
EaeI y'GGCC_r
Cuts at: 627 5063 5237 5628 5655 7000 627
Size: 4436 174 391 27 1345 1105
Fragments arranged by size:
4436 1345 1105 391 174 27
EagI C'GGCC_G
Cuts at: 5063 5063
Size: 7478
EarI CTCTTCn'nnn_
Cuts at: 787 2219 2982 3086 4031 5501 5711 787
Size: 1432 763 104 945 1470 210 2554
Fragments arranged by size:
2554 1470 1432 945 763 210 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 161 1102 3965 5686 5996 6142 7288 161
Size: 941 2863 1721 310 146 1146 351
Fragments arranged by size:
2863 1721 1146 941 351 310 146
EcoICRI GAG'CTC
Cuts at: 671 909 2992 4490 671
Size: 238 2083 1498 3659
Fragments arranged by size:
3659 2083 1498 238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 302 425 811 1874 3064 3878 4073 5303
Size: 9 123 386 1063 1190 814 195 1230
Cuts at: 5303 5735 6482 6750 293
Size: 432 747 268 1021
Fragments arranged by size:
1230 1190 1063 1021 814 747 432 386
268 195 123 9
EcoO109I rG'GnC_Cy
Cuts at: 2287 4617 2287
Size: 2330 5148
EcoRI G'AATT_C
Cuts at: 4482 4482
Size: 7478
EcoRV GAT'ATC
Cuts at: 1098 1098
Size: 7478
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1953 3202 5497 1953
Size: 1249 2295 3934
Fragments arranged by size:
3934 2295 1249
FspI TGC'GCA
Cuts at: 5259 5259
Size: 7478
HaeII r_GCGC'y
Cuts at: 993 4912 5160 6182 7161 993
Size: 3919 248 1022 979 1310
Fragments arranged by size:
3919 1310 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 187 219 253 285 384 416 682 714 1488
Size: 32 34 32 99 32 266 32 774
Cuts at: 1488 1520 1957 1989 2376 2408 5320 5350 5352
Size: 32 437 32 387 32 2912 30 2
Cuts at: 5352 5382 187
Size: 30 2283
Fragments arranged by size:
2912 2283 774 437 387 266 99 34
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3528 4517 3528
Size: 989 6489
HpaI GTT'AAC
Cuts at: 3528 3528
Size: 7478
KasI G'GCGC_C
Cuts at: 4908 5156 4908
Size: 248 7230
KpnI G_GTAC'C
Cuts at: 739 4498 739
Size: 3759 3719
MfeI C'AATT_G
Cuts at: 4214 4214
Size: 7478
MluI A'CGCG_T
Cuts at: 6911 6911
Size: 7478
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1656 1855 2071 3853 6149 6333 1656
Size: 199 216 1782 2296 184 2801
Fragments arranged by size:
2801 2296 1782 216 199 184
MscI TGG'CCA
Cuts at: 629 5239 629
Size: 4610 2868
MslI CAynn'nnrTG
Cuts at: 1777 2705 5467 5594 1777
Size: 928 2762 127 3661
Fragments arranged by size:
3661 2762 928 127
MspA1I CmG'CkG
Cuts at: 1255 1399 3058 4589 4903 5263 6276 6521 1255
Size: 144 1659 1531 314 360 1013 245 2212
Fragments arranged by size:
2212 1659 1531 1013 360 314 245 144
NaeI GCC'GGC
Cuts at: 5659 5659
Size: 7478
NarI GG'CG_CC
Cuts at: 4909 5157 4909
Size: 248 7230
NcoI C'CATG_G
Cuts at: 125 125
Size: 7478
NdeI CA'TA_TG
Cuts at: 553 647 768 553
Size: 94 121 7263
Fragments arranged by size:
7263 121 94
NgoMIV G'CCGG_C
Cuts at: 5657 5657
Size: 7478
NheI G'CTAG_C
Cuts at: 1135 1135
Size: 7478
NspI r_CATG'y
Cuts at: 889 1838 3055 4531 5031 5938 889
Size: 949 1217 1476 500 907 2429
Fragments arranged by size:
2429 1476 1217 949 907 500
PciI A'CATG_T
Cuts at: 5934 5934
Size: 7478
PfoI T'CCnGG_A
Cuts at: 1916 3489 3624 1916
Size: 1573 135 5770
Fragments arranged by size:
5770 1573 135
PmeI GTTT'AAAC
Cuts at: 4477 4477
Size: 7478
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 114 146 6617 6649 114
Size: 32 6471 32 943
Fragments arranged by size:
6471 943 32 32
PpuMI rG'GwC_Cy
Cuts at: 2287 2287
Size: 7478
PshAI GACnn'nnGTC
Cuts at: 2593 2593
Size: 7478
PsiI TTA'TAA
Cuts at: 454 619 1373 454
Size: 165 754 6559
Fragments arranged by size:
6559 754 165
PstI C_TGCA'G
Cuts at: 4525 5210 4525
Size: 685 6793
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7478
PvuII CAG'CTG
Cuts at: 1255 1399 3058 4903 5263 1255
Size: 144 1659 1845 360 3470
Fragments arranged by size:
3470 1845 1659 360 144
RsrII CG'GwC_CG
Cuts at: 5673 5673
Size: 7478
SacI G_AGCT'C
Cuts at: 673 911 2994 4492 673
Size: 238 2083 1498 3659
Fragments arranged by size:
3659 2083 1498 238
SalI G'TCGA_C
Cuts at: 4515 4515
Size: 7478
SapI GCTCTTCn'nnn_
Cuts at: 2982 4031 5501 5711 2982
Size: 1049 1470 210 4749
Fragments arranged by size:
4749 1470 1049 210
SbfI CC_TGCA'GG
Cuts at: 4525 4525
Size: 7478
ScaI AGT'ACT
Cuts at: 1820 3366 6995 1820
Size: 1546 3629 2303
Fragments arranged by size:
3629 2303 1546
SfcI C'TryA_G
Cuts at: 33 1535 4424 4521 5206 6199 6390 33
Size: 1502 2889 97 685 993 191 1121
Fragments arranged by size:
2889 1502 1121 993 685 191 97
SfoI GGC'GCC
Cuts at: 4910 5158 4910
Size: 248 7230
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7478
SmaI CCC'GGG
Cuts at: 4500 4500
Size: 7478
SmlI C'TyrA_G
Cuts at: 331 520 803 2083 2676 2935 4633 5548 6040
Size: 189 283 1280 593 259 1698 915 492
Cuts at: 6040 6302 331
Size: 262 1507
Fragments arranged by size:
1698 1507 1280 915 593 492 283 262
259 189
SnaBI TAC'GTA
Cuts at: 4470 4470
Size: 7478
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7478
SphI G_CATG'C
Cuts at: 3055 4531 5031 3055
Size: 1476 500 5502
Fragments arranged by size:
5502 1476 500
SspI AAT'ATT
Cuts at: 918 2696 3206 3264 918
Size: 1778 510 58 5132
Fragments arranged by size:
5132 1778 510 58
StuI AGG'CCT
Cuts at: 2912 2912
Size: 7478
StyI C'CwwG_G
Cuts at: 125 3285 4612 4966 125
Size: 3160 1327 354 2637
Fragments arranged by size:
3160 2637 1327 354
SwaI ATTT'AAAT
Cuts at: 963 1583 963
Size: 620 6858
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 604 5061 5194 5653 5862 604
Size: 4457 133 459 209 2220
Fragments arranged by size:
4457 2220 459 209 133
TatI w'GTAC_w
Cuts at: 1818 3147 3320 3364 6993 1818
Size: 1329 173 44 3629 2303
Fragments arranged by size:
3629 2303 1329 173 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3702 3734 6995 7027 3702
Size: 32 3261 32 4153
Fragments arranged by size:
4153 3261 32 32
Tth111I GACn'n_nGTC
Cuts at: 5275 5275
Size: 7478
XbaI T'CTAG_A
Cuts at: 4509 4509
Size: 7478
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4973 4973
Size: 7478
XmaI C'CCGG_G
Cuts at: 4498 4498
Size: 7478
XmnI GAAnn'nnTTC
Cuts at: 1768 1768
Size: 7478
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NspI PciI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BsaXI BsiWI BssHII
BstEII BstXI BstZ17I ClaI CspCI EcoNI FseI FspAI
HindIII NotI NruI NsiI PacI PasI PflMI PmlI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI XhoI ZraI