Restriction map for
pF1KSDA0977
(Circular) (Six-base) MAPSORT of: pF1KSDA0977.seq Check: 8477 from: 1 to: 6637
pF1KSDA0977 6637 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 3673 3673
Size: 6637
Acc65I G'GTAC_C
Cuts at: 3651 3651
Size: 6637
AclI AA'CG_TT
Cuts at: 6477 6477
Size: 6637
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 1298 3092 3163 4460 4892 5641 5909 212
Size: 1086 1794 71 1297 432 749 268 940
Fragments arranged by size:
1794 1297 1086 940 749 432 268 71
AflII C'TTAA_G
Cuts at: 2172 2994 2172
Size: 822 5815
AflIII A'CryG_T
Cuts at: 3987 5093 6070 3987
Size: 1106 977 4554
Fragments arranged by size:
4554 1106 977
AgeI A'CCGG_T
Cuts at: 5025 5025
Size: 6637
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1406 1406
Size: 6637
AlwNI CAG_nnn'CTG
Cuts at: 235 3940 5509 235
Size: 3705 1569 1363
Fragments arranged by size:
3705 1569 1363
ApaI G_GGCC'C
Cuts at: 1783 1783
Size: 6637
ApaLI G'TGCA_C
Cuts at: 389 1386 2273 5407 389
Size: 997 887 3134 1619
Fragments arranged by size:
3134 1619 997 887
ApoI r'AATT_y
Cuts at: 457 1185 2206 2322 2760 2794 3325 3390 3639
Size: 728 1021 116 438 34 531 65 249
Cuts at: 3639 5990 6246 457
Size: 2351 256 848
Fragments arranged by size:
2351 1021 848 728 531 438 256 249
116 65 34
AseI AT'TA_AT
Cuts at: 20 578 20
Size: 558 6079
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6637
AvaI C'yCGr_G
Cuts at: 2804 3655 2804
Size: 851 5786
BamHI G'GATC_C
Cuts at: 3660 3660
Size: 6637
BanI G'GyrC_C
Cuts at: 186 2221 3163 3651 4065 4313 4348 186
Size: 2035 942 488 414 248 35 2475
Fragments arranged by size:
2475 2035 942 488 414 248 35
BanII G_rGCy'C
Cuts at: 1011 1783 2680 3649 4679 1011
Size: 772 897 969 1030 2969
Fragments arranged by size:
2969 1030 969 897 772
BbeI G_GCGC'C
Cuts at: 4069 4317 4069
Size: 248 6389
BbsI GAAGACnn'nnnn_
Cuts at: 604 1841 3151 3313 604
Size: 1237 1310 162 3928
Fragments arranged by size:
3928 1310 1237 162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3491 3525 3491
Size: 34 6603
BciVI GTATCCnnnnn_n'
Cuts at: 3138 4533 5296 6637 3138
Size: 1395 763 1341 3138
Fragments arranged by size:
3138 1395 1341 763
BclI T'GATC_A
Cuts at: 2511 4155 2511
Size: 1644 4993
BfrBI ATG'CAT
Cuts at: 625 2523 625
Size: 1898 4739
BglII A'GATC_T
Cuts at: 4150 6189 4150
Size: 2039 4598
BlpI GC'TnA_GC
Cuts at: 1165 3747 1165
Size: 2582 4055
Bme1580I G_kGCm'C
Cuts at: 393 897 1390 1432 1783 2277 2666 3168 4260
Size: 504 493 42 351 494 389 502 1092
Cuts at: 4260 4353 5411 393
Size: 93 1058 1619
Fragments arranged by size:
1619 1092 1058 504 502 494 493 389
351 93 42
BmrI ACTGGGnnnn_n'
Cuts at: 2015 2660 3847 3928 4263 6409 2015
Size: 645 1187 81 335 2146 2243
Fragments arranged by size:
2243 2146 1187 645 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1025 1431 5002 1025
Size: 406 3571 2660
Fragments arranged by size:
3571 2660 406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 1286 3269 182
Size: 1104 1983 3550
Fragments arranged by size:
3550 1983 1104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2184 3165 3811 4690 5184 5482 2184
Size: 981 646 879 494 298 3339
Fragments arranged by size:
3339 981 879 646 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 199 872 1914 6341 199
Size: 673 1042 4427 495
Fragments arranged by size:
4427 1042 673 495
BsaAI yAC'GTr
Cuts at: 3627 3988 3627
Size: 361 6276
BsaBI GATnn'nnATC
Cuts at: 6 4149 6188 6
Size: 4143 2039 455
Fragments arranged by size:
4143 2039 455
BsaHI Gr'CG_yC
Cuts at: 155 4066 4314 5018 6514 155
Size: 3911 248 704 1496 278
Fragments arranged by size:
3911 1496 704 278 248
BsaWI w'CCGG_w
Cuts at: 2973 3824 4345 5025 5299 5446 5857 2973
Size: 851 521 680 274 147 411 3753
Fragments arranged by size:
3753 851 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2973 3003 2973
Size: 30 6607
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1348 3074 1348
Size: 1726 4911
BseYI C'CCAG_C
Cuts at: 159 2830 4060 4818 5397 5893 159
Size: 2671 1230 758 579 496 903
Fragments arranged by size:
2671 1230 903 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2045 3359 2045
Size: 1314 5323
BsiEI CG_ry'CG
Cuts at: 104 135 4177 4223 5433 104
Size: 31 4042 46 1210 1308
Fragments arranged by size:
4042 1308 1210 46 31
BsiHKAI G_wGCw'C
Cuts at: 393 1390 2277 2680 3649 4427 4617 5411 393
Size: 997 887 403 969 778 190 794 1619
Fragments arranged by size:
1619 997 969 887 794 778 403 190
Bsp1286I G_dGCh'C
Cuts at: 393 897 1011 1390 1432 1783 2277 2666 2680
Size: 504 114 379 42 351 494 389 14
Cuts at: 2680 3168 3649 4260 4353 4427 4617 4679 5411
Size: 488 481 611 93 74 190 62 732
Cuts at: 5411 393
Size: 1619
Fragments arranged by size:
1619 732 611 504 494 488 481 389
379 351 190 114 93 74 62 42
14
BspEI T'CCGG_A
Cuts at: 2973 5857 2973
Size: 2884 3753
BspHI T'CATG_A
Cuts at: 2547 5813 2547
Size: 3266 3371
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1141 2721 3685 3899 4201 4582 1141
Size: 1580 964 214 302 381 3196
Fragments arranged by size:
3196 1580 964 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4927 4981 6467 6632 50
Size: 4877 54 1486 165 55
Fragments arranged by size:
4877 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1641 1740 4547 1641
Size: 99 2807 3731
Fragments arranged by size:
3731 2807 99
BsrFI r'CCGG_y
Cuts at: 4633 4814 5025 4633
Size: 181 211 6245
Fragments arranged by size:
6245 211 181
BssSI C'ACGA_G
Cuts at: 2405 4906 5266 2405
Size: 2501 360 3776
Fragments arranged by size:
3776 2501 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2661 4012 2661
Size: 1351 5286
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6637
BstEII G'GTnAC_C
Cuts at: 3540 3540
Size: 6637
BstXI CCAn_nnnn'nTGG
Cuts at: 1104 1404 2463 3273 1104
Size: 300 1059 810 4468
Fragments arranged by size:
4468 1059 810 300
BstYI r'GATC_y
Cuts at: 1 2244 2262 3660 4127 4150 4485 4731 5745
Size: 2243 18 1398 467 23 335 246 1014
Cuts at: 5745 5831 5843 6189 1
Size: 86 12 346 449
Fragments arranged by size:
2243 1398 1014 467 449 346 335 246
86 23 18 12
Bsu36I CC'TnA_GG
Cuts at: 1331 1442 1331
Size: 111 6526
BtgI C'CryG_G
Cuts at: 125 1664 2693 125
Size: 1539 1029 4069
Fragments arranged by size:
4069 1539 1029
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 611 784 4585 4766 4929 611
Size: 173 3801 181 163 2319
Fragments arranged by size:
3801 2319 181 173 163
BtsI GCAGTG_nn'
Cuts at: 251 1012 1156 1388 1390 2327 2614 3878 251
Size: 761 144 232 2 937 287 1264 3010
Fragments arranged by size:
3010 1264 937 761 287 232 144 2
ClaI AT'CG_AT
Cuts at: 425 425
Size: 6637
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1623 1658 1623
Size: 35 6602
DraI TTT'AAA
Cuts at: 509 2799 3634 509
Size: 2290 835 3512
Fragments arranged by size:
3512 2290 835
DraIII CAC_nnn'GTG
Cuts at: 1714 2877 3262 1714
Size: 1163 385 5089
Fragments arranged by size:
5089 1163 385
DrdI GACnn_nn'nnGTC
Cuts at: 4341 5201 4341
Size: 860 5777
EaeI y'GGCC_r
Cuts at: 132 3618 4220 4394 4785 4812 6159 132
Size: 3486 602 174 391 27 1347 610
Fragments arranged by size:
3486 1347 610 602 391 174 27
EagI C'GGCC_G
Cuts at: 132 4220 132
Size: 4088 2549
EarI CTCTTCn'nnn_
Cuts at: 231 994 1134 1490 2603 2851 4658 4868 231
Size: 763 140 356 1113 248 1807 210 2000
Fragments arranged by size:
2000 1807 1113 763 356 248 210 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4843 5155 5301 6447 4843
Size: 312 146 1146 5033
Fragments arranged by size:
5033 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2678 3647 2678
Size: 969 5668
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 212 1286 1298 3092 3163 3269 4460 4892
Size: 30 1074 12 1794 71 106 1191 432
Cuts at: 4892 5641 5909 182
Size: 749 268 910
Fragments arranged by size:
1794 1191 1074 910 749 432 268 106
71 30 12
EcoO109I rG'GnC_Cy
Cuts at: 3774 3774
Size: 6637
EcoRI G'AATT_C
Cuts at: 1185 2206 3390 3639 1185
Size: 1021 1184 249 4183
Fragments arranged by size:
4183 1184 1021 249
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2266 4654 2266
Size: 2388 4249
FspI TGC'GCA
Cuts at: 4416 4416
Size: 6637
HaeII r_GCGC'y
Cuts at: 4069 4317 5341 6320 4069
Size: 248 1024 979 4386
Fragments arranged by size:
4386 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 418 450 600 632 691 720 723 752 1324
Size: 32 150 32 59 29 3 29 572
Cuts at: 1324 1356 1606 1638 1909 1941 2470 2502 2973
Size: 32 250 32 271 32 529 32 471
Cuts at: 2973 3005 3406 3438 4477 4507 4509 4539 418
Size: 32 401 32 1039 30 2 30 2516
Fragments arranged by size:
2516 1039 572 529 471 401 271 250
150 59 32 32 32 32 32 32
32 32 30 30 29 29 3 2
HincII GTy'rAC
Cuts at: 780 1752 3674 780
Size: 972 1922 3743
Fragments arranged by size:
3743 1922 972
KasI G'GCGC_C
Cuts at: 4065 4313 4065
Size: 248 6389
KpnI G_GTAC'C
Cuts at: 3655 3655
Size: 6637
MluI A'CGCG_T
Cuts at: 6070 6070
Size: 6637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 467 503 965 995 3303 3433 5308 5492 467
Size: 36 462 30 2308 130 1875 184 1612
Fragments arranged by size:
2308 1875 1612 462 184 130 36 30
MscI TGG'CCA
Cuts at: 4396 4396
Size: 6637
MslI CAynn'nnrTG
Cuts at: 1102 1250 1906 2282 2546 2552 3433 4624 4751
Size: 148 656 376 264 6 881 1191 127
Cuts at: 4751 1102
Size: 2988
Fragments arranged by size:
2988 1191 881 656 376 264 148 127
6
MspA1I CmG'CkG
Cuts at: 202 232 439 2890 3746 4060 4420 5435 5680
Size: 30 207 2451 856 314 360 1015 245
Cuts at: 5680 202
Size: 1159
Fragments arranged by size:
2451 1159 1015 856 360 314 245 207
30
NaeI GCC'GGC
Cuts at: 4816 4816
Size: 6637
NarI GG'CG_CC
Cuts at: 4066 4314 4066
Size: 248 6389
NcoI C'CATG_G
Cuts at: 125 1664 2693 125
Size: 1539 1029 4069
Fragments arranged by size:
4069 1539 1029
NgoMIV G'CCGG_C
Cuts at: 4814 4814
Size: 6637
NruI TCG'CGA
Cuts at: 4998 4998
Size: 6637
NsiI A_TGCA'T
Cuts at: 627 2525 627
Size: 1898 4739
NspI r_CATG'y
Cuts at: 625 1176 2523 2527 3688 4188 5097 625
Size: 551 1347 4 1161 500 909 2165
Fragments arranged by size:
2165 1347 1161 909 551 500 4
PasI CC'CwG_GG
Cuts at: 1363 1432 1363
Size: 69 6568
PciI A'CATG_T
Cuts at: 5093 5093
Size: 6637
PflMI CCAn_nnn'nTGG
Cuts at: 1291 3346 3415 1291
Size: 2055 69 4513
Fragments arranged by size:
4513 2055 69
PfoI T'CCnGG_A
Cuts at: 1064 1064
Size: 6637
PmeI GTTT'AAAC
Cuts at: 3634 3634
Size: 6637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1137 1169 3429 3461 5776 5808 1137
Size: 32 2260 32 2315 32 1966
Fragments arranged by size:
2315 2260 1966 32 32 32
PsiI TTA'TAA
Cuts at: 2441 2441
Size: 6637
PspOMI G'GGCC_C
Cuts at: 1779 1779
Size: 6637
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1280 1312 1280
Size: 32 6605
PstI C_TGCA'G
Cuts at: 1163 1182 2889 3682 4367 1163
Size: 19 1707 793 685 3433
Fragments arranged by size:
3433 1707 793 685 19
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6637
PvuII CAG'CTG
Cuts at: 202 232 439 2890 4060 4420 202
Size: 30 207 2451 1170 360 2419
Fragments arranged by size:
2451 2419 1170 360 207 30
RsrII CG'GwC_CG
Cuts at: 4830 4830
Size: 6637
SacI G_AGCT'C
Cuts at: 2680 3649 2680
Size: 969 5668
SalI G'TCGA_C
Cuts at: 3672 3672
Size: 6637
SapI GCTCTTCn'nnn_
Cuts at: 2603 4658 4868 2603
Size: 2055 210 4372
Fragments arranged by size:
4372 2055 210
SbfI CC_TGCA'GG
Cuts at: 3682 3682
Size: 6637
ScaI AGT'ACT
Cuts at: 6154 6154
Size: 6637
SfcI C'TryA_G
Cuts at: 33 242 1159 1178 2303 2885 3212 3678 4363
Size: 209 917 19 1125 582 327 466 685
Cuts at: 4363 5358 5549 33
Size: 995 191 1121
Fragments arranged by size:
1125 1121 995 917 685 582 466 327
209 191 19
SfoI GGC'GCC
Cuts at: 4067 4315 4067
Size: 248 6389
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6637
SmaI CCC'GGG
Cuts at: 3657 3657
Size: 6637
SmlI C'TyrA_G
Cuts at: 2172 2199 2994 3180 3790 4705 5199 5461 2172
Size: 27 795 186 610 915 494 262 3348
Fragments arranged by size:
3348 915 795 610 494 262 186 27
SnaBI TAC'GTA
Cuts at: 3627 3627
Size: 6637
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6637
SphI G_CATG'C
Cuts at: 2527 3688 4188 2527
Size: 1161 500 4976
Fragments arranged by size:
4976 1161 500
SspI AAT'ATT
Cuts at: 1551 2781 1551
Size: 1230 5407
StuI AGG'CCT
Cuts at: 1074 1074
Size: 6637
StyI C'CwwG_G
Cuts at: 125 182 1629 1664 2462 2693 3769 4123 125
Size: 57 1447 35 798 231 1076 354 2639
Fragments arranged by size:
2639 1447 1076 798 354 231 57 35
SwaI ATTT'AAAT
Cuts at: 2799 2799
Size: 6637
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1008 3046 4218 4351 4810 5021 1008
Size: 2038 1172 133 459 211 2624
Fragments arranged by size:
2624 2038 1172 459 211 133
TatI w'GTAC_w
Cuts at: 6152 6152
Size: 6637
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6154 6186 6154
Size: 32 6605
Tth111I GACn'n_nGTC
Cuts at: 4432 4432
Size: 6637
XbaI T'CTAG_A
Cuts at: 3666 3666
Size: 6637
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4130 4130
Size: 6637
XmaI C'CCGG_G
Cuts at: 3655 3655
Size: 6637
XmnI GAAnn'nnTTC
Cuts at: 1355 1355
Size: 6637
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PsiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI AscI AvrII
BaeI BbvCI BglI BmgBI BmtI Bpu10I BsiWI BsmI
BsmBI BsrGI BssHII BstZ17I EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI PacI PmlI
PpuMI PshAI PspXI SacII SanDI SexAI SfiI SgrAI
SrfI XhoI ZraI