Restriction map for
pF1KSDA0969
(Circular) (Six-base) MAPSORT of: pF1KSDA0969.seq Check: 7246 from: 1 to: 6281
pF1KSDA0969 6281 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 2738 3269 3319 2738
Size: 531 50 5700
Fragments arranged by size:
5700 531 50
Acc65I G'GTAC_C
Cuts at: 1107 3297 1107
Size: 2190 4091
AclI AA'CG_TT
Cuts at: 6121 6121
Size: 6281
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 522 1063 1139 4106 4538 5285 5553 522
Size: 541 76 2967 432 747 268 1250
Fragments arranged by size:
2967 1250 747 541 432 268 76
AfeI AGC'GCT
Cuts at: 268 364 268
Size: 96 6185
AflIII A'CryG_T
Cuts at: 497 3633 4737 5714 497
Size: 3136 1104 977 1064
Fragments arranged by size:
3136 1104 1064 977
AgeI A'CCGG_T
Cuts at: 4669 4669
Size: 6281
AleI CACnn'nnGTG
Cuts at: 2621 2621
Size: 6281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2110 2142 2110
Size: 32 6249
AlwNI CAG_nnn'CTG
Cuts at: 1839 3586 5153 1839
Size: 1747 1567 2967
Fragments arranged by size:
2967 1747 1567
ApaI G_GGCC'C
Cuts at: 895 1290 895
Size: 395 5886
ApaLI G'TGCA_C
Cuts at: 5051 5051
Size: 6281
ApoI r'AATT_y
Cuts at: 3285 5634 5890 3285
Size: 2349 256 3676
Fragments arranged by size:
3676 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6281
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6281
AvaI C'yCGr_G
Cuts at: 553 596 746 886 1984 2129 2804 3301 553
Size: 43 150 140 1098 145 675 497 3533
Fragments arranged by size:
3533 1098 675 497 150 145 140 43
BamHI G'GATC_C
Cuts at: 572 1394 2087 3306 572
Size: 822 693 1219 3547
Fragments arranged by size:
3547 1219 822 693
BanI G'GyrC_C
Cuts at: 1107 2615 3024 3241 3297 3711 3959 3994 1107
Size: 1508 409 217 56 414 248 35 3394
Fragments arranged by size:
3394 1508 414 409 248 217 56 35
BanII G_rGCy'C
Cuts at: 553 895 1066 1290 1361 1516 2242 2263 2300
Size: 342 171 224 71 155 726 21 37
Cuts at: 2300 2513 2846 2879 2947 3295 4325 553
Size: 213 333 33 68 348 1030 2509
Fragments arranged by size:
2509 1030 726 348 342 333 224 213
171 155 71 68 37 33 21
BbeI G_GCGC'C
Cuts at: 3245 3715 3963 3245
Size: 470 248 5563
Fragments arranged by size:
5563 470 248
BbsI GAAGACnn'nnnn_
Cuts at: 1127 1127
Size: 6281
BbvCI CC'TCA_GC
Cuts at: 2110 2172 2209 2110
Size: 62 37 6182
Fragments arranged by size:
6182 62 37
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 234 268 234
Size: 34 6247
BciVI GTATCCnnnnn_n'
Cuts at: 433 4179 4940 6281 433
Size: 3746 761 1341 433
Fragments arranged by size:
3746 1341 761 433
BclI T'GATC_A
Cuts at: 3801 3801
Size: 6281
BglI GCCn_nnn'nGGC
Cuts at: 798 2385 2671 2767 2784 798
Size: 1587 286 96 17 4295
Fragments arranged by size:
4295 1587 286 96 17
BglII A'GATC_T
Cuts at: 2078 3796 5833 2078
Size: 1718 2037 2526
Fragments arranged by size:
2526 2037 1718
BlpI GC'TnA_GC
Cuts at: 509 3393 509
Size: 2884 3397
Bme1580I G_kGCm'C
Cuts at: 632 895 1290 1496 1530 1563 2137 2381 2441
Size: 263 395 206 34 33 574 244 60
Cuts at: 2441 3029 3059 3906 3999 5055 632
Size: 588 30 847 93 1056 1858
Fragments arranged by size:
1858 1056 847 588 574 395 263 244
206 93 60 34 33 30
BmgBI CAC'GTC
Cuts at: 2097 2859 3054 2097
Size: 762 195 5324
Fragments arranged by size:
5324 762 195
BmrI ACTGGGnnnn_n'
Cuts at: 1010 1188 1550 2678 3493 3574 3909 6053 1010
Size: 178 362 1128 815 81 335 2144 1238
Fragments arranged by size:
2144 1238 1128 815 362 335 178 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 879 3060 4648 879
Size: 2181 1588 2512
Fragments arranged by size:
2512 2181 1588
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 596 1312 1861 2103 2773 2870 2944 3191
Size: 405 716 549 242 670 97 74 247
Cuts at: 3191 191
Size: 3281
Fragments arranged by size:
3281 716 670 549 405 247 242 97
74
Bpu10I CC'TnA_GC
Cuts at: 445 722 728 2110 2172 2209 2236 2941 445
Size: 277 6 1382 62 37 27 705 3785
Fragments arranged by size:
3785 1382 705 277 62 37 27 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3457 4336 4828 5126 3457
Size: 879 492 298 4612
Fragments arranged by size:
4612 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2001 5985 2001
Size: 3984 2297
BsaAI yAC'GTr
Cuts at: 3273 3634 3273
Size: 361 5920
BsaBI GATnn'nnATC
Cuts at: 6 1208 3103 3795 5832 6
Size: 1202 1895 692 2037 455
Fragments arranged by size:
2037 1895 1202 692 455
BsaHI Gr'CG_yC
Cuts at: 3242 3712 3960 4662 6158 3242
Size: 470 248 702 1496 3365
Fragments arranged by size:
3365 1496 702 470 248
BsaWI w'CCGG_w
Cuts at: 822 852 3470 3991 4669 4943 5090 5501 822
Size: 30 2618 521 678 274 147 411 1602
Fragments arranged by size:
2618 1602 678 521 411 274 147 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2110 2140 2110
Size: 30 6251
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 571 1179 2171 2517 2605 2661 2788 2965 3194
Size: 608 992 346 88 56 127 177 229
Cuts at: 3194 571
Size: 3658
Fragments arranged by size:
3658 992 608 346 229 177 127 88
56
BseYI C'CCAG_C
Cuts at: 217 682 843 926 1052 1504 1725 2846 3706
Size: 465 161 83 126 452 221 1121 860
Cuts at: 3706 4464 5041 5537 217
Size: 758 577 496 961
Fragments arranged by size:
1121 961 860 758 577 496 465 452
221 161 126 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 1798 1990 487
Size: 1311 192 4778
Fragments arranged by size:
4778 1311 192
BsiEI CG_ry'CG
Cuts at: 104 1237 3125 3823 3869 5077 104
Size: 1133 1888 698 46 1208 1308
Fragments arranged by size:
1888 1308 1208 1133 698 46
BsiHKAI G_wGCw'C
Cuts at: 973 1066 1982 2879 3295 4073 4263 5055 973
Size: 93 916 897 416 778 190 792 2199
Fragments arranged by size:
2199 916 897 792 778 416 190 93
BsmI GAATG_Cn'
Cuts at: 2505 2612 2505
Size: 107 6174
BsmBI CGTCTCn'nnnn_
Cuts at: 989 989
Size: 6281
Bsp1286I G_dGCh'C
Cuts at: 553 632 895 973 1066 1290 1361 1496 1516
Size: 79 263 78 93 224 71 135 20
Cuts at: 1516 1530 1563 1982 2137 2242 2263 2300 2381
Size: 14 33 419 155 105 21 37 81
Cuts at: 2381 2441 2513 2846 2879 2947 3029 3059 3295
Size: 60 72 333 33 68 82 30 236
Cuts at: 3295 3906 3999 4073 4263 4325 5055 553
Size: 611 93 74 190 62 730 1779
Fragments arranged by size:
1779 730 611 419 333 263 236 224
190 155 135 105 93 93 82 81
79 78 74 72 71 68 62 60
37 33 33 30 21 20 14
BspEI T'CCGG_A
Cuts at: 5501 5501
Size: 6281
BspHI T'CATG_A
Cuts at: 5457 5457
Size: 6281
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3331 3545 3847 4228 3331
Size: 214 302 381 5384
Fragments arranged by size:
5384 381 302 214
BsrBI CCG'CTC
Cuts at: 50 213 1481 1499 1550 1572 4573 4627 6111
Size: 163 1268 18 51 22 3001 54 1484
Cuts at: 6111 6276 50
Size: 165 55
Fragments arranged by size:
3001 1484 1268 165 163 55 54 51
22 18
BsrDI GCAATG_nn'
Cuts at: 1323 2341 2996 3166 4193 1323
Size: 1018 655 170 1027 3411
Fragments arranged by size:
3411 1027 1018 655 170
BsrFI r'CCGG_y
Cuts at: 959 1010 1257 1769 2060 4279 4460 4669 959
Size: 51 247 512 291 2219 181 209 2571
Fragments arranged by size:
2571 2219 512 291 247 209 181 51
BssSI C'ACGA_G
Cuts at: 2731 4552 4910 2731
Size: 1821 358 4102
Fragments arranged by size:
4102 1821 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3658 3658
Size: 6281
BstBI TT'CG_AA
Cuts at: 110 4642 110
Size: 4532 1749
BstEII G'GTnAC_C
Cuts at: 2472 2472
Size: 6281
BstXI CCAn_nnnn'nTGG
Cuts at: 934 934
Size: 6281
BstYI r'GATC_y
Cuts at: 1 572 605 1394 2078 2087 3306 3773 3796
Size: 571 33 789 684 9 1219 467 23
Cuts at: 3796 4131 4377 5389 5475 5487 5833 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1219 1012 789 684 571 467 449 346
335 246 86 33 23 12 9
Bsu36I CC'TnA_GG
Cuts at: 2948 2948
Size: 6281
BtgI C'CryG_G
Cuts at: 581 1946 3235 581
Size: 1365 1289 3627
Fragments arranged by size:
3627 1365 1289
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 237 4231 4412 4575 237
Size: 3994 181 163 1943
Fragments arranged by size:
3994 1943 181 163
BtsI GCAGTG_nn'
Cuts at: 358 470 1090 1593 1897 2492 3524 358
Size: 112 620 503 304 595 1032 3115
Fragments arranged by size:
3115 1032 620 595 503 304 112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1305 1340 1305
Size: 35 6246
DraI TTT'AAA
Cuts at: 3280 3280
Size: 6281
DraIII CAC_nnn'GTG
Cuts at: 1491 1558 1491
Size: 67 6214
DrdI GACnn_nn'nnGTC
Cuts at: 3987 4845 3987
Size: 858 5423
EaeI y'GGCC_r
Cuts at: 762 961 1234 2198 2663 2692 3866 4040 4431
Size: 199 273 964 465 29 1174 174 391
Cuts at: 4431 4458 5803 762
Size: 27 1345 1240
Fragments arranged by size:
1345 1240 1174 964 465 391 273 199
174 29 27
EagI C'GGCC_G
Cuts at: 1234 3866 1234
Size: 2632 3649
EarI CTCTTCn'nnn_
Cuts at: 328 397 410 4304 4514 328
Size: 69 13 3894 210 2095
Fragments arranged by size:
3894 2095 210 69 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1029 4489 4799 4945 6091 1029
Size: 3460 310 146 1146 1219
Fragments arranged by size:
3460 1219 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1064 2877 3293 1064
Size: 1813 416 4052
Fragments arranged by size:
4052 1813 416
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 522 596 1063 1139 1312 1861 2103 2773
Size: 331 74 467 76 173 549 242 670
Cuts at: 2773 2870 2944 3191 4106 4538 5285 5553 191
Size: 97 74 247 915 432 747 268 919
Fragments arranged by size:
919 915 747 670 549 467 432 331
268 247 242 173 97 76 74 74
EcoNI CCTnn'n_nnAGG
Cuts at: 3135 3135
Size: 6281
EcoO109I rG'GnC_Cy
Cuts at: 201 891 1164 1287 1650 1826 2270 3420 201
Size: 690 273 123 363 176 444 1150 3062
Fragments arranged by size:
3062 1150 690 444 363 273 176 123
EcoRI G'AATT_C
Cuts at: 3285 3285
Size: 6281
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4300 4300
Size: 6281
FspI TGC'GCA
Cuts at: 4062 4062
Size: 6281
HaeII r_GCGC'y
Cuts at: 270 366 1305 3245 3715 3963 4985 5964 270
Size: 96 939 1940 470 248 1022 979 587
Fragments arranged by size:
1940 1022 979 939 587 470 248 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 590 622 1429 1461 4123 4153 4155 4185 590
Size: 32 807 32 2662 30 2 30 2686
Fragments arranged by size:
2686 2662 807 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3320 3320
Size: 6281
KasI G'GCGC_C
Cuts at: 3241 3711 3959 3241
Size: 470 248 5563
Fragments arranged by size:
5563 470 248
KpnI G_GTAC'C
Cuts at: 1111 3301 1111
Size: 2190 4091
MluI A'CGCG_T
Cuts at: 5714 5714
Size: 6281
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2213 2315 2771 4952 5136 2213
Size: 102 456 2181 184 3358
Fragments arranged by size:
3358 2181 456 184 102
MscI TGG'CCA
Cuts at: 4042 4042
Size: 6281
MslI CAynn'nnrTG
Cuts at: 650 1924 2621 4270 4397 650
Size: 1274 697 1649 127 2534
Fragments arranged by size:
2534 1649 1274 697 127
MspA1I CmG'CkG
Cuts at: 847 1341 3078 3392 3706 4066 5079 5324 847
Size: 494 1737 314 314 360 1013 245 1804
Fragments arranged by size:
1804 1737 1013 494 360 314 314 245
NaeI GCC'GGC
Cuts at: 961 1259 4462 961
Size: 298 3203 2780
Fragments arranged by size:
3203 2780 298
NarI GG'CG_CC
Cuts at: 3242 3712 3960 3242
Size: 470 248 5563
Fragments arranged by size:
5563 470 248
NcoI C'CATG_G
Cuts at: 581 581
Size: 6281
NgoMIV G'CCGG_C
Cuts at: 959 1257 4460 959
Size: 298 3203 2780
Fragments arranged by size:
3203 2780 298
NspI r_CATG'y
Cuts at: 517 2791 3150 3334 3834 4741 517
Size: 2274 359 184 500 907 2057
Fragments arranged by size:
2274 2057 907 500 359 184
PasI CC'CwG_GG
Cuts at: 1283 1283
Size: 6281
PciI A'CATG_T
Cuts at: 4737 4737
Size: 6281
PflMI CCAn_nnn'nTGG
Cuts at: 189 582 910 938 1391 1839 189
Size: 393 328 28 453 448 4631
Fragments arranged by size:
4631 453 448 393 328 28
PfoI T'CCnGG_A
Cuts at: 1673 2090 1673
Size: 417 5864
PmeI GTTT'AAAC
Cuts at: 3280 3280
Size: 6281
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1233 1265 2110 2142 5420 5452 1233
Size: 32 845 32 3278 32 2062
Fragments arranged by size:
3278 2062 845 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 201 1164 1650 1826 2270 201
Size: 963 486 176 444 4212
Fragments arranged by size:
4212 963 486 444 176
PshAI GACnn'nnGTC
Cuts at: 2684 2684
Size: 6281
PspOMI G'GGCC_C
Cuts at: 891 1286 891
Size: 395 5886
PspXI vC'TCGA_Gb
Cuts at: 596 746 1984 596
Size: 150 1238 4893
Fragments arranged by size:
4893 1238 150
PstI C_TGCA'G
Cuts at: 1475 1904 2657 3083 3328 4013 1475
Size: 429 753 426 245 685 3743
Fragments arranged by size:
3743 753 685 429 426 245
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6281
PvuII CAG'CTG
Cuts at: 847 1341 3078 3706 4066 847
Size: 494 1737 628 360 3062
Fragments arranged by size:
3062 1737 628 494 360
RsrII CG'GwC_CG
Cuts at: 4476 4476
Size: 6281
SacI G_AGCT'C
Cuts at: 1066 2879 3295 1066
Size: 1813 416 4052
Fragments arranged by size:
4052 1813 416
SalI G'TCGA_C
Cuts at: 3318 3318
Size: 6281
SanDI GG'GwC_CC
Cuts at: 1164 1826 2270 1164
Size: 662 444 5175
Fragments arranged by size:
5175 662 444
SapI GCTCTTCn'nnn_
Cuts at: 328 4304 4514 328
Size: 3976 210 2095
Fragments arranged by size:
3976 2095 210
SbfI CC_TGCA'GG
Cuts at: 3328 3328
Size: 6281
ScaI AGT'ACT
Cuts at: 1174 1216 5798 1174
Size: 42 4582 1657
Fragments arranged by size:
4582 1657 42
SfcI C'TryA_G
Cuts at: 33 1471 1518 1900 2181 2653 3079 3324 4009
Size: 1438 47 382 281 472 426 245 685
Cuts at: 4009 5002 5193 33
Size: 993 191 1121
Fragments arranged by size:
1438 1121 993 685 472 426 382 281
245 191 47
SfiI GGCCn_nnn'nGGCC
Cuts at: 798 2671 798
Size: 1873 4408
SfoI GGC'GCC
Cuts at: 3243 3713 3961 3243
Size: 470 248 5563
Fragments arranged by size:
5563 470 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6281
SgrAI Cr'CCGG_yG
Cuts at: 1010 1010
Size: 6281
SmaI CCC'GGG
Cuts at: 2131 2806 3303 2131
Size: 675 497 5109
Fragments arranged by size:
5109 675 497
SmlI C'TyrA_G
Cuts at: 596 746 1984 3436 4351 4843 5105 596
Size: 150 1238 1452 915 492 262 1772
Fragments arranged by size:
1772 1452 1238 915 492 262 150
SnaBI TAC'GTA
Cuts at: 3273 3273
Size: 6281
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6281
SphI G_CATG'C
Cuts at: 517 2791 3150 3334 3834 517
Size: 2274 359 184 500 2964
Fragments arranged by size:
2964 2274 500 359 184
StuI AGG'CCT
Cuts at: 568 792 3130 568
Size: 224 2338 3719
Fragments arranged by size:
3719 2338 224
StyI C'CwwG_G
Cuts at: 308 581 1646 2300 3415 3769 308
Size: 273 1065 654 1115 354 2820
Fragments arranged by size:
2820 1115 1065 654 354 273
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 696 1077 1518 1569 2056 2618 3139 3864 3997
Size: 381 441 51 487 562 521 725 133
Cuts at: 3997 4456 4665 696
Size: 459 209 2312
Fragments arranged by size:
2312 725 562 521 487 459 441 381
209 133 51
TatI w'GTAC_w
Cuts at: 600 1172 1214 5796 600
Size: 572 42 4582 1085
Fragments arranged by size:
4582 1085 572 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2490 2522 5798 5830 2490
Size: 32 3276 32 2941
Fragments arranged by size:
3276 2941 32 32
Tth111I GACn'n_nGTC
Cuts at: 2893 4078 2893
Size: 1185 5096
XbaI T'CTAG_A
Cuts at: 3312 3312
Size: 6281
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 186 3776 186
Size: 3590 2691
XhoI C'TCGA_G
Cuts at: 596 746 1984 596
Size: 150 1238 4893
Fragments arranged by size:
4893 1238 150
XmaI C'CCGG_G
Cuts at: 2129 2804 3301 2129
Size: 675 497 5109
Fragments arranged by size:
5109 675 497
XmnI GAAnn'nnTTC
Cuts at: 1124 1124
Size: 6281
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AscI AvrII BaeI
BfrBI BmtI BsiWI BsrGI BssHII BstZ17I ClaI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PsiI PsrI SacII SexAI
SrfI SspI SwaI ZraI