Restriction map for
pF1KSDA0964
(Circular) (Six-base) MAPSORT of: pF1KSDA0964.seq Check: 8290 from: 1 to: 6106
pF1KSDA0964 6106 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 961 1113 961
Size: 152 5954
AccI GT'mk_AC
Cuts at: 3142 3142
Size: 6106
Acc65I G'GTAC_C
Cuts at: 3120 3120
Size: 6106
AclI AA'CG_TT
Cuts at: 5946 5946
Size: 6106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 465 2642 3929 4361 5110 5378 465
Size: 2177 1287 432 749 268 1193
Fragments arranged by size:
2177 1287 1193 749 432 268
AflIII A'CryG_T
Cuts at: 3456 4562 5539 3456
Size: 1106 977 4023
Fragments arranged by size:
4023 1106 977
AgeI A'CCGG_T
Cuts at: 1812 4494 1812
Size: 2682 3424
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1174 1174
Size: 6106
AlwNI CAG_nnn'CTG
Cuts at: 799 1341 2014 2670 2757 3019 3409 4978 799
Size: 542 673 656 87 262 390 1569 1927
Fragments arranged by size:
1927 1569 673 656 542 390 262 87
ApaI G_GGCC'C
Cuts at: 1198 1198
Size: 6106
ApaLI G'TGCA_C
Cuts at: 4876 4876
Size: 6106
ApoI r'AATT_y
Cuts at: 2683 3108 5459 5715 2683
Size: 425 2351 256 3074
Fragments arranged by size:
3074 2351 425 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6106
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6106
AvaI C'yCGr_G
Cuts at: 265 2092 2467 2863 3124 265
Size: 1827 375 396 261 3247
Fragments arranged by size:
3247 1827 396 375 261
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1953 1986 1953
Size: 33 6073
BamHI G'GATC_C
Cuts at: 3129 3129
Size: 6106
BanI G'GyrC_C
Cuts at: 369 585 772 1125 1307 2202 3120 3534 3782
Size: 216 187 353 182 895 918 414 248
Cuts at: 3782 3817 369
Size: 35 2658
Fragments arranged by size:
2658 918 895 414 353 248 216 187
182 35
BanII G_rGCy'C
Cuts at: 191 385 532 704 745 1003 1198 3118 4148
Size: 194 147 172 41 258 195 1920 1030
Cuts at: 4148 191
Size: 2149
Fragments arranged by size:
2149 1920 1030 258 195 194 172 147
41
BbeI G_GCGC'C
Cuts at: 776 1311 3538 3786 776
Size: 535 2227 248 3096
Fragments arranged by size:
3096 2227 535 248
BbsI GAAGACnn'nnnn_
Cuts at: 1054 1807 1054
Size: 753 5353
BbvCI CC'TCA_GC
Cuts at: 1021 1053 1750 1021
Size: 32 697 5377
Fragments arranged by size:
5377 697 32
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1162 1196 1670 1704 2793 2827 1162
Size: 34 474 34 1089 34 4441
Fragments arranged by size:
4441 1089 474 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2259 4002 4765 6106 2259
Size: 1743 763 1341 2259
Fragments arranged by size:
2259 1743 1341 763
BclI T'GATC_A
Cuts at: 3624 3624
Size: 6106
BglI GCCn_nnn'nGGC
Cuts at: 253 708 1678 1934 2017 2434 2525 3010 253
Size: 455 970 256 83 417 91 485 3349
Fragments arranged by size:
3349 970 485 455 417 256 91 83
BglII A'GATC_T
Cuts at: 3619 5658 3619
Size: 2039 4067
BlpI GC'TnA_GC
Cuts at: 3216 3216
Size: 6106
Bme1580I G_kGCm'C
Cuts at: 604 881 976 1130 1198 2664 3729 3822 4880
Size: 277 95 154 68 1466 1065 93 1058
Cuts at: 4880 604
Size: 1830
Fragments arranged by size:
1830 1466 1065 1058 277 154 95 93
68
BmrI ACTGGGnnnn_n'
Cuts at: 1503 1730 3316 3397 3732 5878 1503
Size: 227 1586 81 335 2146 1731
Fragments arranged by size:
2146 1731 1586 335 227 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 310 2457 2493 4471 310
Size: 2147 36 1978 1945
Fragments arranged by size:
2147 1978 1945 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 1449 2463 616
Size: 833 1014 4259
Fragments arranged by size:
4259 1014 833
Bpu10I CC'TnA_GC
Cuts at: 1021 1053 1750 1939 1021
Size: 32 697 189 5188
Fragments arranged by size:
5188 697 189 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 2271 2815 3280 4159 4653 4951 1063
Size: 1208 544 465 879 494 298 2218
Fragments arranged by size:
2218 1208 879 544 494 465 298
BsaI GGTCTCn'nnnn_
Cuts at: 2464 2593 2851 5810 2464
Size: 129 258 2959 2760
Fragments arranged by size:
2959 2760 258 129
BsaAI yAC'GTr
Cuts at: 3096 3457 3096
Size: 361 5745
BsaBI GATnn'nnATC
Cuts at: 6 3618 5657 6
Size: 3612 2039 455
Fragments arranged by size:
3612 2039 455
BsaHI Gr'CG_yC
Cuts at: 773 1308 2437 2957 3535 3783 4487 5983 773
Size: 535 1129 520 578 248 704 1496 896
Fragments arranged by size:
1496 1129 896 704 578 535 520 248
BsaWI w'CCGG_w
Cuts at: 1812 2898 3293 3814 4494 4768 4915 5326 1812
Size: 1086 395 521 680 274 147 411 2592
Fragments arranged by size:
2592 1086 680 521 411 395 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 340 370 1630 1660 1693 1723 2323 2353 340
Size: 30 1260 30 33 30 600 30 4093
Fragments arranged by size:
4093 1260 600 33 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 391 1711 2199 2322 2466 391
Size: 1320 488 123 144 4031
Fragments arranged by size:
4031 1320 488 144 123
BseYI C'CCAG_C
Cuts at: 325 391 694 792 945 958 1341 1421 1633
Size: 66 303 98 153 13 383 80 212
Cuts at: 1633 1981 2007 2487 2663 3529 4287 4866 5362
Size: 348 26 480 176 866 758 579 496
Cuts at: 5362 325
Size: 1069
Fragments arranged by size:
1069 866 758 579 496 480 383 348
303 212 176 153 98 80 66 26
13
BsiEI CG_ry'CG
Cuts at: 104 210 1190 1978 2192 3646 3692 4902 104
Size: 106 980 788 214 1454 46 1210 1308
Fragments arranged by size:
1454 1308 1210 980 788 214 106 46
BsiHKAI G_wGCw'C
Cuts at: 745 1153 3118 3896 4086 4880 745
Size: 408 1965 778 190 794 1971
Fragments arranged by size:
1971 1965 794 778 408 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1168 1606 2516 1168
Size: 438 910 4758
Fragments arranged by size:
4758 910 438
Bsp1286I G_dGCh'C
Cuts at: 191 385 532 604 704 745 881 976 1003
Size: 194 147 72 100 41 136 95 27
Cuts at: 1003 1130 1153 1198 2664 3118 3729 3822 3896
Size: 127 23 45 1466 454 611 93 74
Cuts at: 3896 4086 4148 4880 191
Size: 190 62 732 1417
Fragments arranged by size:
1466 1417 732 611 454 194 190 147
136 127 100 95 93 74 72 62
45 41 27 23
BspEI T'CCGG_A
Cuts at: 5326 5326
Size: 6106
BspHI T'CATG_A
Cuts at: 5282 5282
Size: 6106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 230 961 1113 1127 1521 1673 2783 3154 3368
Size: 731 152 14 394 152 1110 371 214
Cuts at: 3368 3670 4051 230
Size: 302 381 2285
Fragments arranged by size:
2285 1110 731 394 381 371 302 214
152 152 14
BsrBI CCG'CTC
Cuts at: 50 684 719 1646 2565 4396 4450 5936 6101
Size: 634 35 927 919 1831 54 1486 165
Cuts at: 6101 50
Size: 55
Fragments arranged by size:
1831 1486 927 919 634 165 55 54
35
BsrDI GCAATG_nn'
Cuts at: 630 4016 630
Size: 3386 2720
BsrFI r'CCGG_y
Cuts at: 419 769 1208 1741 1812 2356 2496 2928 4102
Size: 350 439 533 71 544 140 432 1174
Cuts at: 4102 4283 4494 419
Size: 181 211 2031
Fragments arranged by size:
2031 1174 544 533 439 432 350 211
181 140 71
BssSI C'ACGA_G
Cuts at: 184 1033 4375 4735 184
Size: 849 3342 360 1555
Fragments arranged by size:
3342 1555 849 360
BstAPI GCAn_nnn'nTGC
Cuts at: 3481 3481
Size: 6106
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6106
BstXI CCAn_nnnn'nTGG
Cuts at: 438 475 1486 1516 438
Size: 37 1011 30 5028
Fragments arranged by size:
5028 1011 37 30
BstYI r'GATC_y
Cuts at: 1 3129 3596 3619 3954 4200 5214 5300 5312
Size: 3128 467 23 335 246 1014 86 12
Cuts at: 5312 5658 1
Size: 346 449
Fragments arranged by size:
3128 1014 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 2015 2015
Size: 6106
BtgI C'CryG_G
Cuts at: 233 501 519 1184 1241 1298 233
Size: 268 18 665 57 57 5041
Fragments arranged by size:
5041 665 268 57 57 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4054 4235 4398 4054
Size: 181 163 5762
Fragments arranged by size:
5762 181 163
BtsI GCAGTG_nn'
Cuts at: 792 1593 1627 2664 2939 3347 792
Size: 801 34 1037 275 408 3551
Fragments arranged by size:
3551 1037 801 408 275 34
ClaI AT'CG_AT
Cuts at: 2122 2192 2351 2122
Size: 70 159 5877
Fragments arranged by size:
5877 159 70
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 501 536 600 621 635 656 501
Size: 35 64 21 14 21 5951
Fragments arranged by size:
5951 64 35 21 21 14
DraI TTT'AAA
Cuts at: 3103 3103
Size: 6106
DraIII CAC_nnn'GTG
Cuts at: 1996 1996
Size: 6106
DrdI GACnn_nn'nnGTC
Cuts at: 1929 3810 4670 1929
Size: 1881 860 3365
Fragments arranged by size:
3365 1881 860
EaeI y'GGCC_r
Cuts at: 534 1187 1518 3002 3689 3863 4254 4281 5628
Size: 653 331 1484 687 174 391 27 1347
Cuts at: 5628 534
Size: 1012
Fragments arranged by size:
1484 1347 1012 687 653 391 331 174
27
EagI C'GGCC_G
Cuts at: 1187 3689 1187
Size: 2502 3604
EarI CTCTTCn'nnn_
Cuts at: 1421 2863 2873 4127 4337 1421
Size: 1442 10 1254 210 3190
Fragments arranged by size:
3190 1442 1254 210 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 532 4312 4624 4770 5916 532
Size: 3780 312 146 1146 722
Fragments arranged by size:
3780 1146 722 312 146
EcoICRI GAG'CTC
Cuts at: 743 3116 743
Size: 2373 3733
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 465 616 1449 2463 2642 3929 4361 5110 5378
Size: 151 833 1014 179 1287 432 749 268
Cuts at: 5378 465
Size: 1193
Fragments arranged by size:
1287 1193 1014 833 749 432 268 179
151
EcoNI CCTnn'n_nnAGG
Cuts at: 2167 2167
Size: 6106
EcoO109I rG'GnC_Cy
Cuts at: 1194 1195 2019 2370 2510 2700 2754 2772 3243
Size: 1 824 351 140 190 54 18 471
Cuts at: 3243 1194
Size: 4057
Fragments arranged by size:
4057 824 471 351 190 140 54 18
1
EcoRI G'AATT_C
Cuts at: 3108 3108
Size: 6106
EcoRV GAT'ATC
Cuts at: 2799 2799
Size: 6106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4123 4123
Size: 6106
FspI TGC'GCA
Cuts at: 1219 3885 1219
Size: 2666 3440
HaeII r_GCGC'y
Cuts at: 776 1311 2976 3538 3786 4810 5789 776
Size: 535 1665 562 248 1024 979 1093
Fragments arranged by size:
1665 1093 1024 979 562 535 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1693 1725 2245 2277 2935 2967 3946 3976 3978
Size: 32 520 32 658 32 979 30 2
Cuts at: 3978 4008 1693
Size: 30 3791
Fragments arranged by size:
3791 979 658 520 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3143 3143
Size: 6106
KasI G'GCGC_C
Cuts at: 772 1307 3534 3782 772
Size: 535 2227 248 3096
Fragments arranged by size:
3096 2227 535 248
KpnI G_GTAC'C
Cuts at: 3124 3124
Size: 6106
MluI A'CGCG_T
Cuts at: 5539 5539
Size: 6106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 188 746 1931 4777 4961 188
Size: 558 1185 2846 184 1333
Fragments arranged by size:
2846 1333 1185 558 184
MscI TGG'CCA
Cuts at: 1520 3865 1520
Size: 2345 3761
MslI CAynn'nnrTG
Cuts at: 473 4093 4220 473
Size: 3620 127 2359
Fragments arranged by size:
3620 2359 127
MspA1I CmG'CkG
Cuts at: 315 459 567 607 1186 1223 1300 1469 1606
Size: 144 108 40 579 37 77 169 137
Cuts at: 1606 2784 2843 2850 3215 3529 3889 4904 5149
Size: 1178 59 7 365 314 360 1015 245
Cuts at: 5149 315
Size: 1272
Fragments arranged by size:
1272 1178 1015 579 365 360 314 245
169 144 137 108 77 59 40 37
7
NaeI GCC'GGC
Cuts at: 771 1210 2498 2930 4285 771
Size: 439 1288 432 1355 2592
Fragments arranged by size:
2592 1355 1288 439 432
NarI GG'CG_CC
Cuts at: 773 1308 3535 3783 773
Size: 535 2227 248 3096
Fragments arranged by size:
3096 2227 535 248
NcoI C'CATG_G
Cuts at: 1241 1241
Size: 6106
NgoMIV G'CCGG_C
Cuts at: 769 1208 2496 2928 4283 769
Size: 439 1288 432 1355 2592
Fragments arranged by size:
2592 1355 1288 439 432
NruI TCG'CGA
Cuts at: 4467 4467
Size: 6106
NspI r_CATG'y
Cuts at: 885 1218 1398 2044 3157 3657 4566 885
Size: 333 180 646 1113 500 909 2425
Fragments arranged by size:
2425 1113 909 646 500 333 180
PciI A'CATG_T
Cuts at: 4562 4562
Size: 6106
PflMI CCAn_nnn'nTGG
Cuts at: 289 799 2757 2843 289
Size: 510 1958 86 3552
Fragments arranged by size:
3552 1958 510 86
PfoI T'CCnGG_A
Cuts at: 437 2315 3071 437
Size: 1878 756 3472
Fragments arranged by size:
3472 1878 756
PmeI GTTT'AAAC
Cuts at: 3103 3103
Size: 6106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2602 2634 5245 5277 2602
Size: 32 2611 32 3431
Fragments arranged by size:
3431 2611 32 32
PpuMI rG'GwC_Cy
Cuts at: 2754 2772 2754
Size: 18 6088
PspOMI G'GGCC_C
Cuts at: 1194 1194
Size: 6106
PstI C_TGCA'G
Cuts at: 1124 1588 1718 2552 3151 3836 1124
Size: 464 130 834 599 685 3394
Fragments arranged by size:
3394 834 685 599 464 130
PvuI CG_AT'CG
Cuts at: 104 2192 104
Size: 2088 4018
PvuII CAG'CTG
Cuts at: 315 459 1469 2784 2843 2850 3529 3889 315
Size: 144 1010 1315 59 7 679 360 2532
Fragments arranged by size:
2532 1315 1010 679 360 144 59 7
RsrII CG'GwC_CG
Cuts at: 4299 4299
Size: 6106
SacI G_AGCT'C
Cuts at: 745 3118 745
Size: 2373 3733
SacII CC_GC'GG
Cuts at: 1187 1301 1187
Size: 114 5992
SalI G'TCGA_C
Cuts at: 3141 3141
Size: 6106
SapI GCTCTTCn'nnn_
Cuts at: 4127 4337 4127
Size: 210 5896
SbfI CC_TGCA'GG
Cuts at: 1124 3151 1124
Size: 2027 4079
ScaI AGT'ACT
Cuts at: 1869 5623 1869
Size: 3754 2352
SexAI A'CCwGG_T
Cuts at: 548 548
Size: 6106
SfcI C'TryA_G
Cuts at: 33 1120 1584 1714 2548 2779 3147 3832 4827
Size: 1087 464 130 834 231 368 685 995
Cuts at: 4827 5018 33
Size: 191 1121
Fragments arranged by size:
1121 1087 995 834 685 464 368 231
191 130
SfoI GGC'GCC
Cuts at: 774 1309 3536 3784 774
Size: 535 2227 248 3096
Fragments arranged by size:
3096 2227 535 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6106
SmaI CCC'GGG
Cuts at: 267 3126 267
Size: 2859 3247
SmlI C'TyrA_G
Cuts at: 1078 2286 2467 2830 3259 4174 4668 4930 1078
Size: 1208 181 363 429 915 494 262 2254
Fragments arranged by size:
2254 1208 915 494 429 363 262 181
SnaBI TAC'GTA
Cuts at: 3096 3096
Size: 6106
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6106
SphI G_CATG'C
Cuts at: 1218 3157 3657 1218
Size: 1939 500 3667
Fragments arranged by size:
3667 1939 500
StuI AGG'CCT
Cuts at: 134 241 1495 2950 134
Size: 107 1254 1455 3290
Fragments arranged by size:
3290 1455 1254 107
StyI C'CwwG_G
Cuts at: 512 581 689 963 1061 1241 3238 3592 512
Size: 69 108 274 98 180 1997 354 3026
Fragments arranged by size:
3026 1997 354 274 180 108 98 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 220 1305 3687 3820 4279 4490 220
Size: 1085 2382 133 459 211 1836
Fragments arranged by size:
2382 1836 1085 459 211 133
TatI w'GTAC_w
Cuts at: 1867 5621 1867
Size: 3754 2352
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 340 372 2224 2256 2323 2355 2563 2595 5623
Size: 32 1852 32 67 32 208 32 3028
Cuts at: 5623 5655 340
Size: 32 791
Fragments arranged by size:
3028 1852 791 208 67 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1705 3901 1705
Size: 2196 3910
XbaI T'CTAG_A
Cuts at: 3135 3135
Size: 6106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 499 966 2459 3599 499
Size: 467 1493 1140 3006
Fragments arranged by size:
3006 1493 1140 467
XhoI C'TCGA_G
Cuts at: 2467 2467
Size: 6106
XmaI C'CCGG_G
Cuts at: 265 3124 265
Size: 2859 3247
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BfrBI BmgBI BmtI BsgI BsiWI BsmI BsrGI BssHII
BstEII BstZ17I FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NsiI PacI PasI PmlI PshAI PsiI
PspXI PsrI SanDI SfiI SgrAI SrfI SspI SwaI
XmnI ZraI