Restriction map for
pF1KSDA0963
(Circular) (Six-base) MAPSORT of: pF1KSDA0963.seq Check: 8489 from: 1 to: 7235
pF1KSDA0963 7235 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1022 3186 3222 1022
Size: 2164 36 5035
Fragments arranged by size:
5035 2164 36
AatII G_ACGT'C
Cuts at: 3680 3680
Size: 7235
AccI GT'mk_AC
Cuts at: 1376 1631 4273 1376
Size: 255 2642 4338
Fragments arranged by size:
4338 2642 255
Acc65I G'GTAC_C
Cuts at: 4251 4251
Size: 7235
AclI AA'CG_TT
Cuts at: 7075 7075
Size: 7235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1946 2287 3289 3733 5060 5492 6239 6507 1946
Size: 341 1002 444 1327 432 747 268 2674
Fragments arranged by size:
2674 1327 1002 747 444 432 341 268
AfeI AGC'GCT
Cuts at: 1750 2485 1750
Size: 735 6500
AflIII A'CryG_T
Cuts at: 1670 4587 5691 6668 1670
Size: 2917 1104 977 2237
Fragments arranged by size:
2917 2237 1104 977
AgeI A'CCGG_T
Cuts at: 2651 5623 2651
Size: 2972 4263
AhdI GACnn_n'nnGTC
Cuts at: 465 465
Size: 7235
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1129 1351 3712 1129
Size: 222 2361 4652
Fragments arranged by size:
4652 2361 222
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2701 2733 2701
Size: 32 7203
AlwNI CAG_nnn'CTG
Cuts at: 1242 2277 2448 2949 3548 3600 3786 4540 6107
Size: 1035 171 501 599 52 186 754 1567
Cuts at: 6107 1242
Size: 2370
Fragments arranged by size:
2370 1567 1035 754 599 501 186 171
52
ApaI G_GGCC'C
Cuts at: 143 864 2760 2841 3313 143
Size: 721 1896 81 472 4065
Fragments arranged by size:
4065 1896 721 472 81
ApaLI G'TGCA_C
Cuts at: 1114 2587 3055 6005 1114
Size: 1473 468 2950 2344
Fragments arranged by size:
2950 2344 1473 468
ApoI r'AATT_y
Cuts at: 4239 6588 6844 4239
Size: 2349 256 4630
Fragments arranged by size:
4630 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7235
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7235
AvaI C'yCGr_G
Cuts at: 1805 2018 2137 2239 2535 2834 3211 3754 4165
Size: 213 119 102 296 299 377 543 411
Cuts at: 4165 4255 1805
Size: 90 4785
Fragments arranged by size:
4785 543 411 377 299 296 213 119
102 90
BamHI G'GATC_C
Cuts at: 2203 4260 2203
Size: 2057 5178
BanI G'GyrC_C
Cuts at: 1393 1507 1898 2579 2830 3215 3611 3822 3957
Size: 114 391 681 251 385 396 211 135
Cuts at: 3957 4000 4207 4251 4665 4913 4948 1393
Size: 43 207 44 414 248 35 3680
Fragments arranged by size:
3680 681 414 396 391 385 251 248
211 207 135 114 44 43 35
BanII G_rGCy'C
Cuts at: 143 307 864 2186 2336 2760 2841 3313 3500
Size: 164 557 1322 150 424 81 472 187
Cuts at: 3500 4249 5279 143
Size: 749 1030 2099
Fragments arranged by size:
2099 1322 1030 749 557 472 424 187
164 150 81
BbeI G_GCGC'C
Cuts at: 1511 2583 3826 3961 4211 4669 4917 1511
Size: 1072 1243 135 250 458 248 3829
Fragments arranged by size:
3829 1243 1072 458 250 248 135
BbsI GAAGACnn'nnnn_
Cuts at: 2676 3231 3721 2676
Size: 555 490 6190
Fragments arranged by size:
6190 555 490
BbvCI CC'TCA_GC
Cuts at: 689 2800 689
Size: 2111 5124
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 526 560 1207 1241 1536 1570 1811 1845 526
Size: 34 647 34 295 34 241 34 5916
Fragments arranged by size:
5916 647 295 241 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2896 5133 5894 7235 2896
Size: 2237 761 1341 2896
Fragments arranged by size:
2896 2237 1341 761
BclI T'GATC_A
Cuts at: 4755 4755
Size: 7235
BglI GCCn_nnn'nGGC
Cuts at: 358 2300 3670 358
Size: 1942 1370 3923
Fragments arranged by size:
3923 1942 1370
BglII A'GATC_T
Cuts at: 3176 4750 6787 3176
Size: 1574 2037 3624
Fragments arranged by size:
3624 2037 1574
BlpI GC'TnA_GC
Cuts at: 577 1123 1567 4347 577
Size: 546 444 2780 3465
Fragments arranged by size:
3465 2780 546 444
Bme1580I G_kGCm'C
Cuts at: 143 719 864 1118 1453 1901 2591 2760 2841
Size: 576 145 254 335 448 690 169 81
Cuts at: 2841 2897 3059 3218 3313 4003 4860 4953 6009
Size: 56 162 159 95 690 857 93 1056
Cuts at: 6009 143
Size: 1369
Fragments arranged by size:
1369 1056 857 690 690 576 448 335
254 169 162 159 145 95 93 81
56
BmgBI CAC'GTC
Cuts at: 3000 3807 3000
Size: 807 6428
BmrI ACTGGGnnnn_n'
Cuts at: 2183 4447 4528 4863 7007 2183
Size: 2264 81 335 2144 2411
Fragments arranged by size:
2411 2264 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 444 5602 444
Size: 5158 2077
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 264 354 1024 1723 2269 2514 2764 2930 3070
Size: 90 670 699 546 245 250 166 140
Cuts at: 3070 3457 4093 264
Size: 387 636 3406
Fragments arranged by size:
3406 699 670 636 546 387 250 245
166 140 90
Bpu10I CC'TnA_GC
Cuts at: 401 689 2800 2866 401
Size: 288 2111 66 4770
Fragments arranged by size:
4770 2111 288 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1045 4411 5290 5782 6080 1045
Size: 3366 879 492 298 2200
Fragments arranged by size:
3366 2200 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 718 784 2219 2670 2765 6939 718
Size: 66 1435 451 95 4174 1014
Fragments arranged by size:
4174 1435 1014 451 95 66
BsaAI yAC'GTr
Cuts at: 741 2463 4227 4588 741
Size: 1722 1764 361 3388
Fragments arranged by size:
3388 1764 1722 361
BsaBI GATnn'nnATC
Cuts at: 6 4749 6786 6
Size: 4743 2037 455
Fragments arranged by size:
4743 2037 455
BsaHI Gr'CG_yC
Cuts at: 1163 1271 1508 1580 2066 2580 3281 3677 3823
Size: 108 237 72 486 514 701 396 146
Cuts at: 3823 3958 3974 3986 4010 4208 4666 4914 5616
Size: 135 16 12 24 198 458 248 702
Cuts at: 5616 7112 1163
Size: 1496 1286
Fragments arranged by size:
1496 1286 702 701 514 486 458 396
248 237 198 146 135 108 72 24
16 12
BsaWI w'CCGG_w
Cuts at: 1460 2651 4424 4945 5623 5897 6044 6455 1460
Size: 1191 1773 521 678 274 147 411 2240
Fragments arranged by size:
2240 1773 1191 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2113 2143 2593 2623 2650 2680 2701 2731 2113
Size: 30 450 30 27 30 21 30 6617
Fragments arranged by size:
6617 450 30 30 30 30 27 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 399 438 688 691 706 709 712 721 1486
Size: 39 250 3 15 3 3 9 765
Cuts at: 1486 1612 1825 2003 2121 3187 3199 3499 4208
Size: 126 213 178 118 1066 12 300 709
Cuts at: 4208 399
Size: 3426
Fragments arranged by size:
3426 1066 765 709 300 250 213 178
126 118 39 15 12 9 3 3
3
BseYI C'CCAG_C
Cuts at: 711 925 1614 1881 2298 2346 2357 3358 3996
Size: 214 689 267 417 48 11 1001 638
Cuts at: 3996 4122 4660 5418 5995 6491 711
Size: 126 538 758 577 496 1455
Fragments arranged by size:
1455 1001 758 689 638 577 538 496
417 267 214 126 48 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 679 1465 2827 4078 214
Size: 465 786 1362 1251 3371
Fragments arranged by size:
3371 1362 1251 786 465
BsiEI CG_ry'CG
Cuts at: 104 1023 1688 1922 2199 2559 2622 2643 3423
Size: 919 665 234 277 360 63 21 780
Cuts at: 3423 3920 4777 4823 6031 104
Size: 497 857 46 1208 1308
Fragments arranged by size:
1308 1208 919 857 780 665 497 360
277 234 63 46 21
BsiHKAI G_wGCw'C
Cuts at: 307 1118 2336 2591 3059 4249 5027 5217 6009
Size: 811 1218 255 468 1190 778 190 792
Cuts at: 6009 307
Size: 1533
Fragments arranged by size:
1533 1218 1190 811 792 778 468 255
190
BsmI GAATG_Cn'
Cuts at: 1311 3072 1311
Size: 1761 5474
BsmBI CGTCTCn'nnnn_
Cuts at: 1053 1920 1053
Size: 867 6368
Bsp1286I G_dGCh'C
Cuts at: 143 307 719 864 1118 1453 1901 2186 2336
Size: 164 412 145 254 335 448 285 150
Cuts at: 2336 2591 2760 2841 2897 3059 3218 3313 3500
Size: 255 169 81 56 162 159 95 187
Cuts at: 3500 4003 4249 4860 4953 5027 5217 5279 6009
Size: 503 246 611 93 74 190 62 730
Cuts at: 6009 143
Size: 1369
Fragments arranged by size:
1369 730 611 503 448 412 335 285
255 254 246 190 187 169 164 162
159 150 145 95 93 81 74 62
56
BspEI T'CCGG_A
Cuts at: 1460 6455 1460
Size: 4995 2240
BspHI T'CATG_A
Cuts at: 299 2489 6411 299
Size: 2190 3922 1123
Fragments arranged by size:
3922 2190 1123
BspMI ACCTGCnnnn'nnnn_
Cuts at: 397 1022 1091 1352 3186 3222 3704 4285 4499
Size: 625 69 261 1834 36 482 581 214
Cuts at: 4499 4801 5182 397
Size: 302 381 2450
Fragments arranged by size:
2450 1834 625 581 482 381 302 261
214 69 36
BsrBI CCG'CTC
Cuts at: 50 1993 2253 2262 2495 4170 5527 5581 7065
Size: 1943 260 9 233 1675 1357 54 1484
Cuts at: 7065 7230 50
Size: 165 55
Fragments arranged by size:
1943 1675 1484 1357 260 233 165 55
54 9
BsrDI GCAATG_nn'
Cuts at: 5147 5147
Size: 7235
BsrFI r'CCGG_y
Cuts at: 180 498 964 1207 1309 1787 2294 2377 2651
Size: 318 466 243 102 478 507 83 274
Cuts at: 2651 2714 3422 3554 3563 3976 5233 5414 5623
Size: 63 708 132 9 413 1257 181 209
Cuts at: 5623 180
Size: 1792
Fragments arranged by size:
1792 1257 708 507 478 466 413 318
274 243 209 181 132 102 83 63
9
BsrGI T'GTAC_A
Cuts at: 197 1550 3404 197
Size: 1353 1854 4028
Fragments arranged by size:
4028 1854 1353
BssHII G'CGCG_C
Cuts at: 936 1864 3632 4118 936
Size: 928 1768 486 4053
Fragments arranged by size:
4053 1768 928 486
BssSI C'ACGA_G
Cuts at: 910 2506 5506 5864 910
Size: 1596 3000 358 2281
Fragments arranged by size:
3000 2281 1596 358
BstAPI GCAn_nnn'nTGC
Cuts at: 657 2277 2949 3600 3705 3786 4612 657
Size: 1620 672 651 105 81 826 3280
Fragments arranged by size:
3280 1620 826 672 651 105 81
BstBI TT'CG_AA
Cuts at: 110 5596 110
Size: 5486 1749
BstEII G'GTnAC_C
Cuts at: 3474 3474
Size: 7235
BstXI CCAn_nnnn'nTGG
Cuts at: 1210 1323 2742 3297 1210
Size: 113 1419 555 5148
Fragments arranged by size:
5148 1419 555 113
BstYI r'GATC_y
Cuts at: 1 1238 1601 2203 2274 3176 3749 4260 4727
Size: 1237 363 602 71 902 573 511 467
Cuts at: 4727 4750 5085 5331 6343 6429 6441 6787 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1237 1012 902 602 573 511 467 449
363 346 335 246 86 71 23 12
Bsu36I CC'TnA_GG
Cuts at: 2470 2470
Size: 7235
BtgI C'CryG_G
Cuts at: 146 356 455 1100 1511 1526 1853 2608 2766
Size: 210 99 645 411 15 327 755 158
Cuts at: 2766 2783 3261 3345 3384 3479 146
Size: 17 478 84 39 95 3902
Fragments arranged by size:
3902 755 645 478 411 327 210 158
99 95 84 39 17 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 971 1092 1539 1755 3642 5185 5366 5529 971
Size: 121 447 216 1887 1543 181 163 2677
Fragments arranged by size:
2677 1887 1543 447 216 181 163 121
BtsI GCAGTG_nn'
Cuts at: 140 231 3531 3558 4478 140
Size: 91 3300 27 920 2897
Fragments arranged by size:
3300 2897 920 91 27
DraI TTT'AAA
Cuts at: 4234 4234
Size: 7235
DrdI GACnn_nn'nnGTC
Cuts at: 1537 2161 2563 4941 5799 1537
Size: 624 402 2378 858 2973
Fragments arranged by size:
2973 2378 858 624 402
EaeI y'GGCC_r
Cuts at: 359 989 1020 1212 1523 1685 1919 2510 2640
Size: 630 31 192 311 162 234 591 130
Cuts at: 2640 2702 2732 2970 3387 3420 3671 3917 4820
Size: 62 30 238 417 33 251 246 903
Cuts at: 4820 4994 5385 5412 6757 359
Size: 174 391 27 1345 837
Fragments arranged by size:
1345 903 837 630 591 417 391 311
251 246 238 234 192 174 162 130
62 33 31 30 27
EagI C'GGCC_G
Cuts at: 1020 1685 1919 2640 3420 3917 4820 1020
Size: 665 234 721 780 497 903 3435
Fragments arranged by size:
3435 903 780 721 665 497 234
EarI CTCTTCn'nnn_
Cuts at: 1174 1489 5258 5468 1174
Size: 315 3769 210 2941
Fragments arranged by size:
3769 2941 315 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 297 718 1173 2383 3388 3445 5443 5753 5899
Size: 421 455 1210 1005 57 1998 310 146
Cuts at: 5899 7045 297
Size: 1146 487
Fragments arranged by size:
1998 1210 1146 1005 487 455 421 310
146 57
EcoICRI GAG'CTC
Cuts at: 305 2334 4247 305
Size: 2029 1913 3293
Fragments arranged by size:
3293 2029 1913
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 264 354 1024 1723 1946 2269 2287 2514 2764
Size: 90 670 699 223 323 18 227 250
Cuts at: 2764 2930 3070 3289 3457 3733 4093 5060 5492
Size: 166 140 219 168 276 360 967 432
Cuts at: 5492 6239 6507 264
Size: 747 268 992
Fragments arranged by size:
992 967 747 699 670 432 360 323
276 268 250 227 223 219 168 166
140 90 18
EcoNI CCTnn'n_nnAGG
Cuts at: 514 514
Size: 7235
EcoO109I rG'GnC_Cy
Cuts at: 139 860 861 915 1662 2244 2756 2757 2838
Size: 721 1 54 747 582 512 1 81
Cuts at: 2838 2916 2946 3310 3441 4374 139
Size: 78 30 364 131 933 3000
Fragments arranged by size:
3000 933 747 721 582 512 364 131
81 78 54 30 1 1
EcoRI G'AATT_C
Cuts at: 4239 4239
Size: 7235
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2502 2829 5254 2502
Size: 327 2425 4483
Fragments arranged by size:
4483 2425 327
FspI TGC'GCA
Cuts at: 5016 5016
Size: 7235
HaeII r_GCGC'y
Cuts at: 224 1388 1511 1752 2487 2583 2618 2715 2742
Size: 1164 123 241 735 96 35 97 27
Cuts at: 2742 3304 3548 3826 3871 3961 4211 4669 4917
Size: 562 244 278 45 90 250 458 248
Cuts at: 4917 5939 6918 224
Size: 1022 979 541
Fragments arranged by size:
1164 1022 979 735 562 541 458 278
250 248 244 241 123 97 96 90
45 35 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 451 483 2113 2145 2188 2220 5077 5107 5109
Size: 32 1630 32 43 32 2857 30 2
Cuts at: 5109 5139 451
Size: 30 2547
Fragments arranged by size:
2857 2547 1630 43 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2319 4274 2319
Size: 1955 5280
KasI G'GCGC_C
Cuts at: 1507 2579 3822 3957 4207 4665 4913 1507
Size: 1072 1243 135 250 458 248 3829
Fragments arranged by size:
3829 1243 1072 458 250 248 135
KpnI G_GTAC'C
Cuts at: 4255 4255
Size: 7235
MluI A'CGCG_T
Cuts at: 6668 6668
Size: 7235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 449 455 1076 2081 2681 5906 6090 449
Size: 6 621 1005 600 3225 184 1594
Fragments arranged by size:
3225 1594 1005 621 600 184 6
MscI TGG'CCA
Cuts at: 361 1214 1525 2512 2734 4996 361
Size: 853 311 987 222 2262 2600
Fragments arranged by size:
2600 2262 987 853 311 222
MslI CAynn'nnrTG
Cuts at: 1111 1321 1531 3112 5224 5351 1111
Size: 210 210 1581 2112 127 2995
Fragments arranged by size:
2995 2112 1581 210 210 127
MspA1I CmG'CkG
Cuts at: 857 929 1544 1608 1924 2062 2346 2361 2577
Size: 72 615 64 316 138 284 15 216
Cuts at: 2577 3263 3347 3362 3386 3435 3481 3864 3963
Size: 686 84 15 24 49 46 383 99
Cuts at: 3963 3996 4346 4660 5020 6033 6278 857
Size: 33 350 314 360 1013 245 1814
Fragments arranged by size:
1814 1013 686 615 383 360 350 316
314 284 245 216 138 99 84 72
64 49 46 33 24 15 15
NaeI GCC'GGC
Cuts at: 182 500 966 1311 1789 2296 3424 3565 3978
Size: 318 466 345 478 507 1128 141 413
Cuts at: 3978 5416 182
Size: 1438 2001
Fragments arranged by size:
2001 1438 1128 507 478 466 413 345
318 141
NarI GG'CG_CC
Cuts at: 1508 2580 3823 3958 4208 4666 4914 1508
Size: 1072 1243 135 250 458 248 3829
Fragments arranged by size:
3829 1243 1072 458 250 248 135
NcoI C'CATG_G
Cuts at: 146 1511 1526 146
Size: 1365 15 5855
Fragments arranged by size:
5855 1365 15
NgoMIV G'CCGG_C
Cuts at: 180 498 964 1309 1787 2294 3422 3563 3976
Size: 318 466 345 478 507 1128 141 413
Cuts at: 3976 5414 180
Size: 1438 2001
Fragments arranged by size:
2001 1438 1128 507 478 466 413 345
318 141
NspI r_CATG'y
Cuts at: 1551 3624 4083 4288 4788 5695 1551
Size: 2073 459 205 500 907 3091
Fragments arranged by size:
3091 2073 907 500 459 205
PasI CC'CwG_GG
Cuts at: 2898 3500 3831 2898
Size: 602 331 6302
Fragments arranged by size:
6302 602 331
PciI A'CATG_T
Cuts at: 5691 5691
Size: 7235
PflMI CCAn_nnn'nTGG
Cuts at: 530 2364 530
Size: 1834 5401
PfoI T'CCnGG_A
Cuts at: 1001 1241 2246 2930 2980 3149 1001
Size: 240 1005 684 50 169 5087
Fragments arranged by size:
5087 1005 684 240 169 50
PmeI GTTT'AAAC
Cuts at: 4234 4234
Size: 7235
PmlI CAC'GTG
Cuts at: 2463 2463
Size: 7235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 238 270 2701 2733 6374 6406 238
Size: 32 2431 32 3641 32 1067
Fragments arranged by size:
3641 2431 1067 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 915 2244 2916 915
Size: 1329 672 5234
Fragments arranged by size:
5234 1329 672
PshAI GACnn'nnGTC
Cuts at: 1289 1289
Size: 7235
PspOMI G'GGCC_C
Cuts at: 139 860 2756 2837 3309 139
Size: 721 1896 81 472 4065
Fragments arranged by size:
4065 1896 721 472 81
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1402 1434 1402
Size: 32 7203
PstI C_TGCA'G
Cuts at: 218 878 1349 1850 2222 3538 3596 3701 3782
Size: 660 471 501 372 1316 58 105 81
Cuts at: 3782 4282 4967 218
Size: 500 685 2486
Fragments arranged by size:
2486 1316 685 660 501 500 471 372
105 81 58
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7235
PvuII CAG'CTG
Cuts at: 2346 3362 3435 4660 5020 2346
Size: 1016 73 1225 360 4561
Fragments arranged by size:
4561 1225 1016 360 73
RsrII CG'GwC_CG
Cuts at: 5430 5430
Size: 7235
SacI G_AGCT'C
Cuts at: 307 2336 4249 307
Size: 2029 1913 3293
Fragments arranged by size:
3293 2029 1913
SacII CC_GC'GG
Cuts at: 3264 3348 3387 3482 3264
Size: 84 39 95 7017
Fragments arranged by size:
7017 95 84 39
SalI G'TCGA_C
Cuts at: 4272 4272
Size: 7235
SanDI GG'GwC_CC
Cuts at: 2916 2916
Size: 7235
SapI GCTCTTCn'nnn_
Cuts at: 5258 5468 5258
Size: 210 7025
SbfI CC_TGCA'GG
Cuts at: 3596 4282 3596
Size: 686 6549
ScaI AGT'ACT
Cuts at: 3082 6752 3082
Size: 3670 3565
SexAI A'CCwGG_T
Cuts at: 515 2660 515
Size: 2145 5090
SfcI C'TryA_G
Cuts at: 33 214 874 1345 1846 2218 3534 3592 3697
Size: 181 660 471 501 372 1316 58 105
Cuts at: 3697 3778 3906 4278 4963 5956 6147 33
Size: 81 128 372 685 993 191 1121
Fragments arranged by size:
1316 1121 993 685 660 501 471 372
372 191 181 128 105 81 58
SfoI GGC'GCC
Cuts at: 1509 2581 3824 3959 4209 4667 4915 1509
Size: 1072 1243 135 250 458 248 3829
Fragments arranged by size:
3829 1243 1072 458 250 248 135
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7235
SgrAI Cr'CCGG_yG
Cuts at: 180 180
Size: 7235
SmaI CCC'GGG
Cuts at: 2241 2836 3213 4257 2241
Size: 595 377 1044 5219
Fragments arranged by size:
5219 1044 595 377
SmlI C'TyrA_G
Cuts at: 1060 4390 5305 5797 6059 1060
Size: 3330 915 492 262 2236
Fragments arranged by size:
3330 2236 915 492 262
SnaBI TAC'GTA
Cuts at: 4227 4227
Size: 7235
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7235
SphI G_CATG'C
Cuts at: 4288 4788 4288
Size: 500 6735
StuI AGG'CCT
Cuts at: 1261 2527 3925 1261
Size: 1266 1398 4571
Fragments arranged by size:
4571 1398 1266
StyI C'CwwG_G
Cuts at: 146 1511 1526 1778 2597 3491 3719 4369 4723
Size: 1365 15 252 819 894 228 650 354
Cuts at: 4723 146
Size: 2658
Fragments arranged by size:
2658 1365 894 819 650 354 252 228
15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 571 788 2662 4818 4951 5410 5619 571
Size: 217 1874 2156 133 459 209 2187
Fragments arranged by size:
2187 2156 1874 459 217 209 133
TatI w'GTAC_w
Cuts at: 197 1550 2807 3080 3404 6750 197
Size: 1353 1257 273 324 3346 682
Fragments arranged by size:
3346 1353 1257 682 324 273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1948 1980 2593 2625 6752 6784 1948
Size: 32 613 32 4127 32 2399
Fragments arranged by size:
4127 2399 613 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2161 5032 2161
Size: 2871 4364
XbaI T'CTAG_A
Cuts at: 4266 4266
Size: 7235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 1518 2905 3366 4730 132
Size: 1386 1387 461 1364 2637
Fragments arranged by size:
2637 1387 1386 1364 461
XmaI C'CCGG_G
Cuts at: 2239 2834 3211 4255 2239
Size: 595 377 1044 5219
Fragments arranged by size:
5219 1044 595 377
XmnI GAAnn'nnTTC
Cuts at: 250 1672 250
Size: 1422 5813
ZraI GAC'GTC
Cuts at: 3678 3678
Size: 7235
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AleI AscI AvrII BaeI BfrBI BmtI BsiWI
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PsiI PspXI SfiI SrfI SspI SwaI XhoI