Restriction map for
pF1KSDA0962
(Circular) (Six-base) MAPSORT of: pF1KSDA0962.seq Check: 14 from: 1 to: 5485
pF1KSDA0962 5485 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 1391 2060 2521 1391
Size: 669 461 4355
Fragments arranged by size:
4355 669 461
Acc65I G'GTAC_C
Cuts at: 2499 2499
Size: 5485
AclI AA'CG_TT
Cuts at: 5325 5325
Size: 5485
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1628 3308 3740 4489 4757 1628
Size: 1680 432 749 268 2356
Fragments arranged by size:
2356 1680 749 432 268
AflIII A'CryG_T
Cuts at: 2835 3941 4918 2835
Size: 1106 977 3402
Fragments arranged by size:
3402 1106 977
AgeI A'CCGG_T
Cuts at: 3873 3873
Size: 5485
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1430 1430
Size: 5485
AlwNI CAG_nnn'CTG
Cuts at: 2788 4357 2788
Size: 1569 3916
ApaLI G'TGCA_C
Cuts at: 4255 4255
Size: 5485
ApoI r'AATT_y
Cuts at: 188 477 1549 2016 2044 2143 2487 4838 5094
Size: 289 1072 467 28 99 344 2351 256
Cuts at: 5094 188
Size: 579
Fragments arranged by size:
2351 1072 579 467 344 289 256 99
28
AseI AT'TA_AT
Cuts at: 20 20
Size: 5485
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5485
AvaI C'yCGr_G
Cuts at: 284 1151 2339 2503 284
Size: 867 1188 164 3266
Fragments arranged by size:
3266 1188 867 164
AvrII C'CTAG_G
Cuts at: 225 753 780 225
Size: 528 27 4930
Fragments arranged by size:
4930 528 27
BamHI G'GATC_C
Cuts at: 2365 2508 2365
Size: 143 5342
BanI G'GyrC_C
Cuts at: 2499 2913 3161 3196 2499
Size: 414 248 35 4788
Fragments arranged by size:
4788 414 248 35
BanII G_rGCy'C
Cuts at: 2431 2497 3527 2431
Size: 66 1030 4389
Fragments arranged by size:
4389 1030 66
BbeI G_GCGC'C
Cuts at: 2917 3165 2917
Size: 248 5237
BbsI GAAGACnn'nnnn_
Cuts at: 332 850 1526 1867 332
Size: 518 676 341 3950
Fragments arranged by size:
3950 676 518 341
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 636 670 1621 1655 1770 1804 636
Size: 34 951 34 115 34 4317
Fragments arranged by size:
4317 951 115 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3381 4144 5485 3381
Size: 763 1341 3381
Fragments arranged by size:
3381 1341 763
BclI T'GATC_A
Cuts at: 393 3003 393
Size: 2610 2875
BglI GCCn_nnn'nGGC
Cuts at: 250 250
Size: 5485
BglII A'GATC_T
Cuts at: 2998 5037 2998
Size: 2039 3446
BlpI GC'TnA_GC
Cuts at: 2595 2595
Size: 5485
Bme1580I G_kGCm'C
Cuts at: 762 3108 3201 4259 762
Size: 2346 93 1058 1988
Fragments arranged by size:
2346 1988 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1874 2081 2695 2776 3111 5257 1874
Size: 207 614 81 335 2146 2102
Fragments arranged by size:
2146 2102 614 335 207 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3850 3850
Size: 5485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 431 1743 1820 2443 338
Size: 93 1312 77 623 3380
Fragments arranged by size:
3380 1312 623 93 77
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1349 2135 2659 3538 4032 4330 1349
Size: 786 524 879 494 298 2504
Fragments arranged by size:
2504 879 786 524 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 5189 5189
Size: 5485
BsaAI yAC'GTr
Cuts at: 2475 2836 2475
Size: 361 5124
BsaBI GATnn'nnATC
Cuts at: 6 2997 5036 6
Size: 2991 2039 455
Fragments arranged by size:
2991 2039 455
BsaHI Gr'CG_yC
Cuts at: 738 2914 3162 3866 5362 738
Size: 2176 248 704 1496 861
Fragments arranged by size:
2176 1496 861 704 248
BsaWI w'CCGG_w
Cuts at: 2672 3193 3873 4147 4294 4705 2672
Size: 521 680 274 147 411 3452
Fragments arranged by size:
3452 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1527 1557 1527
Size: 30 5455
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2296 2296
Size: 5485
BseYI C'CCAG_C
Cuts at: 724 1697 2908 3666 4245 4741 724
Size: 973 1211 758 579 496 1468
Fragments arranged by size:
1468 1211 973 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2322 2322
Size: 5485
BsiEI CG_ry'CG
Cuts at: 104 1264 3025 3071 4281 104
Size: 1160 1761 46 1210 1308
Fragments arranged by size:
1761 1308 1210 1160 46
BsiHKAI G_wGCw'C
Cuts at: 2497 3275 3465 4259 2497
Size: 778 190 794 3723
Fragments arranged by size:
3723 794 778 190
BsmI GAATG_Cn'
Cuts at: 647 647
Size: 5485
BsmBI CGTCTCn'nnnn_
Cuts at: 398 729 398
Size: 331 5154
Bsp1286I G_dGCh'C
Cuts at: 762 2431 2497 3108 3201 3275 3465 3527 4259
Size: 1669 66 611 93 74 190 62 732
Cuts at: 4259 762
Size: 1988
Fragments arranged by size:
1988 1669 732 611 190 93 74 66
62
BspEI T'CCGG_A
Cuts at: 4705 4705
Size: 5485
BspHI T'CATG_A
Cuts at: 4661 4661
Size: 5485
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2533 2747 3049 3430 2533
Size: 214 302 381 4588
Fragments arranged by size:
4588 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3775 3829 5315 5480 50
Size: 3725 54 1486 165 55
Fragments arranged by size:
3725 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3395 3395
Size: 5485
BsrFI r'CCGG_y
Cuts at: 3481 3662 3873 3481
Size: 181 211 5093
Fragments arranged by size:
5093 211 181
BssSI C'ACGA_G
Cuts at: 3754 4114 3754
Size: 360 5125
BstAPI GCAn_nnn'nTGC
Cuts at: 2860 2860
Size: 5485
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5485
BstXI CCAn_nnnn'nTGG
Cuts at: 1968 1968
Size: 5485
BstYI r'GATC_y
Cuts at: 1 312 1587 2365 2508 2975 2998 3333 3579
Size: 311 1275 778 143 467 23 335 246
Cuts at: 3579 4593 4679 4691 5037 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1275 1014 778 467 449 346 335 311
246 143 86 23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 5485
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3433 3614 3777 3433
Size: 181 163 5141
Fragments arranged by size:
5141 181 163
BtsI GCAGTG_nn'
Cuts at: 1619 2310 2726 1619
Size: 691 416 4378
Fragments arranged by size:
4378 691 416
DraI TTT'AAA
Cuts at: 1113 2482 1113
Size: 1369 4116
DrdI GACnn_nn'nnGTC
Cuts at: 3189 4049 3189
Size: 860 4625
EaeI y'GGCC_r
Cuts at: 2232 3068 3242 3633 3660 5007 2232
Size: 836 174 391 27 1347 2710
Fragments arranged by size:
2710 1347 836 391 174 27
EagI C'GGCC_G
Cuts at: 3068 3068
Size: 5485
EarI CTCTTCn'nnn_
Cuts at: 1052 1601 1771 1780 2260 2273 2322 3506 3716
Size: 549 170 9 480 13 49 1184 210
Cuts at: 3716 1052
Size: 2821
Fragments arranged by size:
2821 1184 549 480 210 170 49 13
9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 451 3691 4003 4149 5295 451
Size: 3240 312 146 1146 641
Fragments arranged by size:
3240 1146 641 312 146
EcoICRI GAG'CTC
Cuts at: 2495 2495
Size: 5485
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 431 1628 1743 1820 2443 3308 3740 4489
Size: 93 1197 115 77 623 865 432 749
Cuts at: 4489 4757 338
Size: 268 1066
Fragments arranged by size:
1197 1066 865 749 623 432 268 115
93 77
EcoNI CCTnn'n_nnAGG
Cuts at: 1916 2422 2436 1916
Size: 506 14 4965
Fragments arranged by size:
4965 506 14
EcoO109I rG'GnC_Cy
Cuts at: 2622 2622
Size: 5485
EcoRI G'AATT_C
Cuts at: 2016 2487 2016
Size: 471 5014
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1633 2262 3502 1633
Size: 629 1240 3616
Fragments arranged by size:
3616 1240 629
FspI TGC'GCA
Cuts at: 3264 3264
Size: 5485
HaeII r_GCGC'y
Cuts at: 2917 3165 4189 5168 2917
Size: 248 1024 979 3234
Fragments arranged by size:
3234 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1003 1035 1672 1704 1810 1842 2299 2331 3325
Size: 32 637 32 106 32 457 32 994
Cuts at: 3325 3355 3357 3387 1003
Size: 30 2 30 3101
Fragments arranged by size:
3101 994 637 457 106 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 992 2313 2522 992
Size: 1321 209 3955
Fragments arranged by size:
3955 1321 209
HindIII A'AGCT_T
Cuts at: 1881 1881
Size: 5485
KasI G'GCGC_C
Cuts at: 2913 3161 2913
Size: 248 5237
KpnI G_GTAC'C
Cuts at: 2503 2503
Size: 5485
MfeI C'AATT_G
Cuts at: 536 536
Size: 5485
MluI A'CGCG_T
Cuts at: 4918 4918
Size: 5485
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 182 4156 4340 182
Size: 3974 184 1327
Fragments arranged by size:
3974 1327 184
MscI TGG'CCA
Cuts at: 2234 3244 2234
Size: 1010 4475
MslI CAynn'nnrTG
Cuts at: 130 470 1994 1996 3472 3599 130
Size: 340 1524 2 1476 127 2016
Fragments arranged by size:
2016 1524 1476 340 127 2
MspA1I CmG'CkG
Cuts at: 1462 1578 2081 2594 2908 3268 4283 4528 1462
Size: 116 503 513 314 360 1015 245 2419
Fragments arranged by size:
2419 1015 513 503 360 314 245 116
NaeI GCC'GGC
Cuts at: 3664 3664
Size: 5485
NarI GG'CG_CC
Cuts at: 2914 3162 2914
Size: 248 5237
NcoI C'CATG_G
Cuts at: 125 125
Size: 5485
NgoMIV G'CCGG_C
Cuts at: 3662 3662
Size: 5485
NruI TCG'CGA
Cuts at: 3846 3846
Size: 5485
NspI r_CATG'y
Cuts at: 1390 2536 3036 3945 1390
Size: 1146 500 909 2930
Fragments arranged by size:
2930 1146 909 500
PciI A'CATG_T
Cuts at: 3941 3941
Size: 5485
PfoI T'CCnGG_A
Cuts at: 315 315
Size: 5485
PmeI GTTT'AAAC
Cuts at: 2482 2482
Size: 5485
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4624 4656 4624
Size: 32 5453
PsiI TTA'TAA
Cuts at: 272 272
Size: 5485
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 409 441 409
Size: 32 5453
PstI C_TGCA'G
Cuts at: 2530 3215 2530
Size: 685 4800
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5485
PvuII CAG'CTG
Cuts at: 1578 2081 2908 3268 1578
Size: 503 827 360 3795
Fragments arranged by size:
3795 827 503 360
RsrII CG'GwC_CG
Cuts at: 3678 3678
Size: 5485
SacI G_AGCT'C
Cuts at: 2497 2497
Size: 5485
SalI G'TCGA_C
Cuts at: 2520 2520
Size: 5485
SapI GCTCTTCn'nnn_
Cuts at: 1601 3506 3716 1601
Size: 1905 210 3370
Fragments arranged by size:
3370 1905 210
SbfI CC_TGCA'GG
Cuts at: 2530 2530
Size: 5485
ScaI AGT'ACT
Cuts at: 1282 5002 1282
Size: 3720 1765
SfcI C'TryA_G
Cuts at: 33 1392 2038 2526 3211 4206 4397 33
Size: 1359 646 488 685 995 191 1121
Fragments arranged by size:
1359 1121 995 685 646 488 191
SfoI GGC'GCC
Cuts at: 2915 3163 2915
Size: 248 5237
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5485
SmaI CCC'GGG
Cuts at: 286 2505 286
Size: 2219 3266
SmlI C'TyrA_G
Cuts at: 1364 2150 2339 2638 3553 4047 4309 1364
Size: 786 189 299 915 494 262 2540
Fragments arranged by size:
2540 915 786 494 299 262 189
SnaBI TAC'GTA
Cuts at: 2475 2475
Size: 5485
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5485
SphI G_CATG'C
Cuts at: 2536 3036 2536
Size: 500 4985
StuI AGG'CCT
Cuts at: 1131 1131
Size: 5485
StyI C'CwwG_G
Cuts at: 125 225 753 780 1525 2361 2617 2971 125
Size: 100 528 27 745 836 256 354 2639
Fragments arranged by size:
2639 836 745 528 354 256 100 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 688 1645 2320 3066 3199 3658 3869 688
Size: 957 675 746 133 459 211 2304
Fragments arranged by size:
2304 957 746 675 459 211 133
TatI w'GTAC_w
Cuts at: 1280 2217 5000 1280
Size: 937 2783 1765
Fragments arranged by size:
2783 1765 937
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 698 730 1269 1301 1487 1519 1521 1527 1553
Size: 32 539 32 186 32 2 6 26
Cuts at: 1553 1559 5002 5034 698
Size: 6 3443 32 1149
Fragments arranged by size:
3443 1149 539 186 32 32 32 32
26 6 6 2
Tth111I GACn'n_nGTC
Cuts at: 3280 3280
Size: 5485
XbaI T'CTAG_A
Cuts at: 2106 2514 2106
Size: 408 5077
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1861 2978 1861
Size: 1117 4368
XhoI C'TCGA_G
Cuts at: 2339 2339
Size: 5485
XmaI C'CCGG_G
Cuts at: 284 2503 284
Size: 2219 3266
XmnI GAAnn'nnTTC
Cuts at: 584 677 857 1234 2245 584
Size: 93 180 377 1011 3824
Fragments arranged by size:
3824 1011 377 180 93
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PciI PfoI PmeI PpiI PsiI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI NdeI NheI NotI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SrfI SspI SwaI
ZraI