Restriction map for
pF1KSDA0934
(Circular) (Six-base) MAPSORT of: pF1KSDA0934.seq Check: 5235 from: 1 to: 7807
pF1KSDA0934 7807 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2887 4745 2887
Size: 1858 5949
AatII G_ACGT'C
Cuts at: 398 710 1763 3440 398
Size: 312 1053 1677 4765
Fragments arranged by size:
4765 1677 1053 312
AccI GT'mk_AC
Cuts at: 2591 2730 3224 4079 4843 2591
Size: 139 494 855 764 5555
Fragments arranged by size:
5555 855 764 494 139
Acc65I G'GTAC_C
Cuts at: 4484 4821 4484
Size: 337 7470
AclI AA'CG_TT
Cuts at: 7647 7647
Size: 7807
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 347 3181 3525 5630 6062 6811 7079 347
Size: 2834 344 2105 432 749 268 1075
Fragments arranged by size:
2834 2105 1075 749 432 344 268
AfeI AGC'GCT
Cuts at: 382 382
Size: 7807
AflIII A'CryG_T
Cuts at: 1627 3809 4790 5157 6263 7240 1627
Size: 2182 981 367 1106 977 2194
Fragments arranged by size:
2194 2182 1106 981 977 367
AgeI A'CCGG_T
Cuts at: 6195 6195
Size: 7807
AhdI GACnn_n'nnGTC
Cuts at: 2062 2062
Size: 7807
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 172 4753 172
Size: 4581 3226
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1241 1273 1834 1866 1241
Size: 32 561 32 7182
Fragments arranged by size:
7182 561 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1032 1695 4063 5110 6679 1032
Size: 663 2368 1047 1569 2160
Fragments arranged by size:
2368 2160 1569 1047 663
ApaI G_GGCC'C
Cuts at: 1026 1026
Size: 7807
ApaLI G'TGCA_C
Cuts at: 3840 6577 3840
Size: 2737 5070
ApoI r'AATT_y
Cuts at: 949 4563 4809 7160 7416 949
Size: 3614 246 2351 256 1340
Fragments arranged by size:
3614 2351 1340 256 246
AscI GG'CGCG_CC
Cuts at: 175 175
Size: 7807
AseI AT'TA_AT
Cuts at: 20 20
Size: 7807
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7807
AvaI C'yCGr_G
Cuts at: 1565 2142 3899 4825 1565
Size: 577 1757 926 4547
Fragments arranged by size:
4547 1757 926 577
BamHI G'GATC_C
Cuts at: 996 1856 2801 4132 4830 996
Size: 860 945 1331 698 3973
Fragments arranged by size:
3973 1331 945 860 698
BanI G'GyrC_C
Cuts at: 571 1108 1125 1368 2763 3698 3722 4484 4821
Size: 537 17 243 1395 935 24 762 337
Cuts at: 4821 5235 5483 5518 571
Size: 414 248 35 2860
Fragments arranged by size:
2860 1395 935 762 537 414 337 248
243 35 24 17
BanII G_rGCy'C
Cuts at: 318 848 1026 1769 2421 2842 3859 4098 4403
Size: 530 178 743 652 421 1017 239 305
Cuts at: 4403 4524 4819 5849 318
Size: 121 295 1030 2276
Fragments arranged by size:
2276 1030 1017 743 652 530 421 305
295 239 178 121
BbeI G_GCGC'C
Cuts at: 1129 5239 5487 1129
Size: 4110 248 3449
Fragments arranged by size:
4110 3449 248
BbsI GAAGACnn'nnnn_
Cuts at: 353 2028 2478 353
Size: 1675 450 5682
Fragments arranged by size:
5682 1675 450
BbvCI CC'TCA_GC
Cuts at: 2291 2291
Size: 7807
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1893 1927 3312 3346 3996 4026 4030 4060 1893
Size: 34 1385 34 650 30 4 30 5640
Fragments arranged by size:
5640 1385 650 34 34 30 30 4
BciVI GTATCCnnnnn_n'
Cuts at: 3456 5703 6466 7807 3456
Size: 2247 763 1341 3456
Fragments arranged by size:
3456 2247 1341 763
BclI T'GATC_A
Cuts at: 1811 5325 1811
Size: 3514 4293
BfrBI ATG'CAT
Cuts at: 1806 1914 2023 2166 1806
Size: 108 109 143 7447
Fragments arranged by size:
7447 143 109 108
BglI GCCn_nnn'nGGC
Cuts at: 146 1315 2369 146
Size: 1169 1054 5584
Fragments arranged by size:
5584 1169 1054
BglII A'GATC_T
Cuts at: 3497 5320 7359 3497
Size: 1823 2039 3945
Fragments arranged by size:
3945 2039 1823
BlpI GC'TnA_GC
Cuts at: 2657 4917 2657
Size: 2260 5547
Bme1580I G_kGCm'C
Cuts at: 574 1026 1111 1361 2350 2872 3725 3844 4263
Size: 452 85 250 989 522 853 119 419
Cuts at: 4263 4365 4452 5430 5523 6581 574
Size: 102 87 978 93 1058 1800
Fragments arranged by size:
1800 1058 989 978 853 522 452 419
250 119 102 93 87 85
BmgBI CAC'GTC
Cuts at: 644 1628 2351 3446 644
Size: 984 723 1095 5005
Fragments arranged by size:
5005 1095 984 723
BmrI ACTGGGnnnn_n'
Cuts at: 3566 3691 5017 5098 5433 7579 3566
Size: 125 1326 81 335 2146 3794
Fragments arranged by size:
3794 2146 1326 335 125 81
BmtI G_CTAG'C
Cuts at: 3568 3568
Size: 7807
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6172 6172
Size: 7807
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 184 211 217 500 567 1105 1286 2273
Size: 21 27 6 283 67 538 181 987
Cuts at: 2273 2854 4291 4666 163
Size: 581 1437 375 3304
Fragments arranged by size:
3304 1437 987 581 538 375 283 181
67 27 21 6
Bpu10I CC'TnA_GC
Cuts at: 2291 2897 2291
Size: 606 7201
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3859 4311 4981 5860 6354 6652 3859
Size: 452 670 879 494 298 5014
Fragments arranged by size:
5014 879 670 494 452 298
BsaI GGTCTCn'nnnn_
Cuts at: 786 847 2961 2984 4498 7511 786
Size: 61 2114 23 1514 3013 1082
Fragments arranged by size:
3013 2114 1514 1082 61 23
BsaAI yAC'GTr
Cuts at: 761 3213 4083 4440 4797 5158 761
Size: 2452 870 357 357 361 3410
Fragments arranged by size:
3410 2452 870 361 357 357
BsaBI GATnn'nnATC
Cuts at: 6 1816 2647 5319 7358 6
Size: 1810 831 2672 2039 455
Fragments arranged by size:
2672 2039 1810 831 455
BsaHI Gr'CG_yC
Cuts at: 395 503 707 1126 1454 1760 3437 4694 5236
Size: 108 204 419 328 306 1677 1257 542
Cuts at: 5236 5484 6188 7684 395
Size: 248 704 1496 518
Fragments arranged by size:
1677 1496 1257 704 542 518 419 328
306 248 204 108
BsaWI w'CCGG_w
Cuts at: 993 2111 4994 5515 6195 6469 6616 7027 993
Size: 1118 2883 521 680 274 147 411 1773
Fragments arranged by size:
2883 1773 1118 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 857 887 1259 1289 1421 1451 1834 1864 2468
Size: 30 372 30 132 30 383 30 604
Cuts at: 2468 2498 4661 4691 857
Size: 30 2163 30 3973
Fragments arranged by size:
3973 2163 604 383 372 132 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 645 1950 4663 645
Size: 1305 2713 3789
Fragments arranged by size:
3789 2713 1305
BseYI C'CCAG_C
Cuts at: 1053 1647 2457 3852 4221 5230 5988 6567 7063
Size: 594 810 1395 369 1009 758 579 496
Cuts at: 7063 1053
Size: 1797
Fragments arranged by size:
1797 1395 1009 810 758 594 579 496
369
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 574 725 2248 2319 2354 2614 2830 3130
Size: 66 151 1523 71 35 260 216 300
Cuts at: 3130 3160 508
Size: 30 5155
Fragments arranged by size:
5155 1523 300 260 216 151 71 66
35 30
BsiEI CG_ry'CG
Cuts at: 104 928 5347 5393 6603 104
Size: 824 4419 46 1210 1308
Fragments arranged by size:
4419 1308 1210 824 46
BsiHKAI G_wGCw'C
Cuts at: 611 776 848 2222 3066 3844 4403 4655 4819
Size: 165 72 1374 844 778 559 252 164
Cuts at: 4819 5597 5787 6581 611
Size: 778 190 794 1837
Fragments arranged by size:
1837 1374 844 794 778 778 559 252
190 165 164 72
BsmI GAATG_Cn'
Cuts at: 1220 1976 1220
Size: 756 7051
BsmBI CGTCTCn'nnnn_
Cuts at: 4289 4736 4289
Size: 447 7360
Bsp1286I G_dGCh'C
Cuts at: 318 574 611 776 848 1026 1111 1361 1769
Size: 256 37 165 72 178 85 250 408
Cuts at: 1769 2222 2350 2421 2842 2872 3066 3725 3844
Size: 453 128 71 421 30 194 659 119
Cuts at: 3844 3859 4098 4263 4365 4403 4452 4524 4655
Size: 15 239 165 102 38 49 72 131
Cuts at: 4655 4819 5430 5523 5597 5787 5849 6581 318
Size: 164 611 93 74 190 62 732 1544
Fragments arranged by size:
1544 732 659 611 453 421 408 256
250 239 194 190 178 165 165 164
131 128 119 102 93 85 74 72
72 71 62 49 38 37 30 15
BspEI T'CCGG_A
Cuts at: 2111 7027 2111
Size: 4916 2891
BspHI T'CATG_A
Cuts at: 1793 6983 1793
Size: 5190 2617
BspMI ACCTGCnnnn'nnnn_
Cuts at: 464 2887 3142 3919 4745 4855 5069 5371 5752
Size: 2423 255 777 826 110 214 302 381
Cuts at: 5752 464
Size: 2519
Fragments arranged by size:
2519 2423 826 777 381 302 255 214
110
BsrBI CCG'CTC
Cuts at: 50 441 674 6097 6151 7637 7802 50
Size: 391 233 5423 54 1486 165 55
Fragments arranged by size:
5423 1486 391 233 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2862 5717 2862
Size: 2855 4952
BsrFI r'CCGG_y
Cuts at: 5803 5984 6195 5803
Size: 181 211 7415
Fragments arranged by size:
7415 211 181
BssHII G'CGCG_C
Cuts at: 175 175
Size: 7807
BssSI C'ACGA_G
Cuts at: 371 2242 6076 6436 371
Size: 1871 3834 360 1742
Fragments arranged by size:
3834 1871 1742 360
BstAPI GCAn_nnn'nTGC
Cuts at: 3509 4731 5182 3509
Size: 1222 451 6134
Fragments arranged by size:
6134 1222 451
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7807
BstEII G'GTnAC_C
Cuts at: 2619 4629 2619
Size: 2010 5797
BstXI CCAn_nnnn'nTGG
Cuts at: 2565 2983 3663 3852 4779 2565
Size: 418 680 189 927 5593
Fragments arranged by size:
5593 927 680 418 189
BstYI r'GATC_y
Cuts at: 1 996 1493 1856 2801 3497 4132 4830 5297
Size: 995 497 363 945 696 635 698 467
Cuts at: 5297 5320 5655 5901 6915 7001 7013 7359 1
Size: 23 335 246 1014 86 12 346 449
Fragments arranged by size:
1014 995 945 698 696 635 497 467
449 363 346 335 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 2731 2731
Size: 7807
Bsu36I CC'TnA_GG
Cuts at: 4056 4056
Size: 7807
BtgI C'CryG_G
Cuts at: 125 630 806 1142 1253 2120 2675 3994 4463
Size: 505 176 336 111 867 555 1319 469
Cuts at: 4463 4713 125
Size: 250 3219
Fragments arranged by size:
3219 1319 867 555 505 469 336 250
176 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2033 3303 5755 5936 6099 2033
Size: 1270 2452 181 163 3741
Fragments arranged by size:
3741 2452 1270 181 163
BtsI GCAGTG_nn'
Cuts at: 458 1093 1604 1647 1683 2558 3061 3631 3714
Size: 635 511 43 36 875 503 570 83
Cuts at: 3714 5048 458
Size: 1334 3217
Fragments arranged by size:
3217 1334 875 635 570 511 503 83
43 36
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4695 4730 4695
Size: 35 7772
DraI TTT'AAA
Cuts at: 1506 4804 1506
Size: 3298 4509
DrdI GACnn_nn'nnGTC
Cuts at: 2349 4693 5511 6371 2349
Size: 2344 818 860 3785
Fragments arranged by size:
3785 2344 860 818
EaeI y'GGCC_r
Cuts at: 182 425 1034 1072 1340 1513 2105 2555 2567
Size: 243 609 38 268 173 592 450 12
Cuts at: 2567 3653 5390 5564 5955 5982 7329 182
Size: 1086 1737 174 391 27 1347 660
Fragments arranged by size:
1737 1347 1086 660 609 592 450 391
268 243 174 173 38 27 12
EagI C'GGCC_G
Cuts at: 5390 5390
Size: 7807
EarI CTCTTCn'nnn_
Cuts at: 235 531 2198 2675 2959 3923 5828 6038 235
Size: 296 1667 477 284 964 1905 210 2004
Fragments arranged by size:
2004 1905 1667 964 477 296 284 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 145 324 718 1999 3940 6013 6325 6471 7617
Size: 179 394 1281 1941 2073 312 146 1146
Cuts at: 7617 145
Size: 335
Fragments arranged by size:
2073 1941 1281 1146 394 335 312 179
146
EcoICRI GAG'CTC
Cuts at: 846 4401 4817 846
Size: 3555 416 3836
Fragments arranged by size:
3836 3555 416
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 184 211 217 347 500 567 1105 1286
Size: 21 27 6 130 153 67 538 181
Cuts at: 1286 2273 2854 3181 3525 4291 4666 5630 6062
Size: 987 581 327 344 766 375 964 432
Cuts at: 6062 6811 7079 163
Size: 749 268 891
Fragments arranged by size:
987 964 891 766 749 581 538 432
375 344 327 268 181 153 130 67
27 21 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1699 2056 3043 1699
Size: 357 987 6463
Fragments arranged by size:
6463 987 357
EcoO109I rG'GnC_Cy
Cuts at: 476 812 1022 1692 2094 2470 3908 3978 4060
Size: 336 210 670 402 376 1438 70 82
Cuts at: 4060 4944 476
Size: 884 3339
Fragments arranged by size:
3339 1438 884 670 402 376 336 210
82 70
EcoRI G'AATT_C
Cuts at: 949 4809 949
Size: 3860 3947
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2403 5824 2403
Size: 3421 4386
FspI TGC'GCA
Cuts at: 5586 5586
Size: 7807
HaeII r_GCGC'y
Cuts at: 157 384 826 1129 4425 5239 5487 6511 7490
Size: 227 442 303 3296 814 248 1024 979
Cuts at: 7490 157
Size: 474
Fragments arranged by size:
3296 1024 979 814 474 442 303 248
227
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 532 564 2048 2080 2197 2229 2468 2500 3677
Size: 32 1484 32 117 32 239 32 1177
Cuts at: 3677 3709 4661 4693 5647 5677 5679 5709 532
Size: 32 952 32 954 30 2 30 2630
Fragments arranged by size:
2630 1484 1177 954 952 239 117 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 4844 4844
Size: 7807
HindIII A'AGCT_T
Cuts at: 293 293
Size: 7807
KasI G'GCGC_C
Cuts at: 1125 5235 5483 1125
Size: 4110 248 3449
Fragments arranged by size:
4110 3449 248
KpnI G_GTAC'C
Cuts at: 4488 4825 4488
Size: 337 7470
MluI A'CGCG_T
Cuts at: 7240 7240
Size: 7807
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 185 2721 4577 4647 6478 6662 185
Size: 2536 1856 70 1831 184 1330
Fragments arranged by size:
2536 1856 1831 1330 184 70
MscI TGG'CCA
Cuts at: 427 1036 1515 2107 2557 3655 5566 427
Size: 609 479 592 450 1098 1911 2668
Fragments arranged by size:
2668 1911 1098 609 592 479 450
MslI CAynn'nnrTG
Cuts at: 601 1366 1798 2191 4777 5794 5921 601
Size: 765 432 393 2586 1017 127 2487
Fragments arranged by size:
2586 2487 1017 765 432 393 127
MspA1I CmG'CkG
Cuts at: 683 1053 2295 2525 3144 3390 3996 4221 4274
Size: 370 1242 230 619 246 606 225 53
Cuts at: 4274 4916 5230 5590 6605 6850 683
Size: 642 314 360 1015 245 1640
Fragments arranged by size:
1640 1242 1015 642 619 606 370 360
314 246 245 230 225 53
NaeI GCC'GGC
Cuts at: 5986 5986
Size: 7807
NarI GG'CG_CC
Cuts at: 1126 5236 5484 1126
Size: 4110 248 3449
Fragments arranged by size:
4110 3449 248
NcoI C'CATG_G
Cuts at: 125 1142 1253 4463 125
Size: 1017 111 3210 3469
Fragments arranged by size:
3469 3210 1017 111
NgoMIV G'CCGG_C
Cuts at: 5984 5984
Size: 7807
NheI G'CTAG_C
Cuts at: 3564 3564
Size: 7807
NruI TCG'CGA
Cuts at: 3322 6168 3322
Size: 2846 4961
NsiI A_TGCA'T
Cuts at: 1808 1916 2025 2168 1808
Size: 108 109 143 7447
Fragments arranged by size:
7447 143 109 108
NspI r_CATG'y
Cuts at: 1810 4482 4734 4794 4858 5358 6267 1810
Size: 2672 252 60 64 500 909 3350
Fragments arranged by size:
3350 2672 909 500 252 64 60
PasI CC'CwG_GG
Cuts at: 567 567
Size: 7807
PciI A'CATG_T
Cuts at: 4790 6263 4790
Size: 1473 6334
PflMI CCAn_nnn'nTGG
Cuts at: 699 1032 699
Size: 333 7474
PfoI T'CCnGG_A
Cuts at: 814 1852 2472 3458 4643 814
Size: 1038 620 986 1185 3978
Fragments arranged by size:
3978 1185 1038 986 620
PmeI GTTT'AAAC
Cuts at: 4804 4804
Size: 7807
PmlI CAC'GTG
Cuts at: 3213 3213
Size: 7807
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1834 1866 2941 2973 6946 6978 1834
Size: 32 1075 32 3973 32 2663
Fragments arranged by size:
3973 2663 1075 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 476 812 2470 3908 3978 4060 476
Size: 336 1658 1438 70 82 4223
Fragments arranged by size:
4223 1658 1438 336 82 70
PshAI GACnn'nnGTC
Cuts at: 1789 2996 4510 1789
Size: 1207 1514 5086
Fragments arranged by size:
5086 1514 1207
PsiI TTA'TAA
Cuts at: 4192 4192
Size: 7807
PspOMI G'GGCC_C
Cuts at: 1022 1022
Size: 7807
PstI C_TGCA'G
Cuts at: 1100 2717 3626 3709 4852 5537 1100
Size: 1617 909 83 1143 685 3370
Fragments arranged by size:
3370 1617 1143 909 685 83
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7807
PvuII CAG'CTG
Cuts at: 1053 2295 3144 3390 5230 5590 1053
Size: 1242 849 246 1840 360 3270
Fragments arranged by size:
3270 1840 1242 849 360 246
RsrII CG'GwC_CG
Cuts at: 132 6000 132
Size: 5868 1939
SacI G_AGCT'C
Cuts at: 848 4403 4819 848
Size: 3555 416 3836
Fragments arranged by size:
3836 3555 416
SacII CC_GC'GG
Cuts at: 3997 3997
Size: 7807
SalI G'TCGA_C
Cuts at: 4842 4842
Size: 7807
SanDI GG'GwC_CC
Cuts at: 812 2470 812
Size: 1658 6149
SapI GCTCTTCn'nnn_
Cuts at: 5828 6038 5828
Size: 210 7597
SbfI CC_TGCA'GG
Cuts at: 4852 4852
Size: 7807
ScaI AGT'ACT
Cuts at: 7324 7324
Size: 7807
SexAI A'CCwGG_T
Cuts at: 2951 4616 2951
Size: 1665 6142
SfcI C'TryA_G
Cuts at: 33 1096 1436 2713 3622 3705 4848 5533 6528
Size: 1063 340 1277 909 83 1143 685 995
Cuts at: 6528 6719 33
Size: 191 1121
Fragments arranged by size:
1277 1143 1121 1063 995 909 685 340
191 83
SfoI GGC'GCC
Cuts at: 1127 5237 5485 1127
Size: 4110 248 3449
Fragments arranged by size:
4110 3449 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7807
SmaI CCC'GGG
Cuts at: 2144 4827 2144
Size: 2683 5124
SmlI C'TyrA_G
Cuts at: 3874 4326 4960 5875 6369 6631 3874
Size: 452 634 915 494 262 5050
Fragments arranged by size:
5050 915 634 494 452 262
SnaBI TAC'GTA
Cuts at: 4797 4797
Size: 7807
SpeI A'CTAG_T
Cuts at: 67 1447 67
Size: 1380 6427
SphI G_CATG'C
Cuts at: 4482 4734 4858 5358 4482
Size: 252 124 500 6931
Fragments arranged by size:
6931 500 252 124
StuI AGG'CCT
Cuts at: 2051 2480 2667 3936 2051
Size: 429 187 1269 5922
Fragments arranged by size:
5922 1269 429 187
StyI C'CwwG_G
Cuts at: 125 1121 1142 1253 2759 3218 3845 4236 4463
Size: 996 21 111 1506 459 627 391 227
Cuts at: 4463 4610 4625 4939 5293 125
Size: 147 15 314 354 2639
Fragments arranged by size:
2639 1506 996 627 459 391 354 314
227 147 111 21 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 377 942 1639 3323 4363 4500 4505 5388 5521
Size: 565 697 1684 1040 137 5 883 133
Cuts at: 5521 5980 6191 377
Size: 459 211 1993
Fragments arranged by size:
1993 1684 1040 883 697 565 459 211
137 133 5
TatI w'GTAC_w
Cuts at: 1619 1874 3794 7322 1619
Size: 255 1920 3528 2104
Fragments arranged by size:
3528 2104 1920 255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1259 1271 1291 1303 2691 2723 3014 3046 7324
Size: 12 20 12 1388 32 291 32 4278
Cuts at: 7324 7356 1259
Size: 32 1710
Fragments arranged by size:
4278 1710 1388 291 32 32 32 20
12 12
Tth111I GACn'n_nGTC
Cuts at: 4111 5602 4111
Size: 1491 6316
XbaI T'CTAG_A
Cuts at: 3643 4836 3643
Size: 1193 6614
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1523 4080 4641 5300 1523
Size: 2557 561 659 4030
Fragments arranged by size:
4030 2557 659 561
XmaI C'CCGG_G
Cuts at: 2142 4825 2142
Size: 2683 5124
ZraI GAC'GTC
Cuts at: 396 708 1761 3438 396
Size: 312 1053 1677 4765
Fragments arranged by size:
4765 1677 1053 312
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NruI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AflII AleI AvrII BaeI BsiWI BsrGI ClaI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NotI PacI
PspXI PsrI SfiI SgrAI SrfI SspI SwaI XhoI
XmnI