Restriction map for
pF1KSDA0921
(Circular) (Six-base) MAPSORT of: pF1KSDA0921.seq Check: 3309 from: 1 to: 8063
pF1KSDA0921 8063 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 549 3258 3310 3370 3714 3932 549
Size: 2709 52 60 344 218 4680
Fragments arranged by size:
4680 2709 344 218 60 52
AatII G_ACGT'C
Cuts at: 1181 3228 1181
Size: 2047 6016
AccI GT'mk_AC
Cuts at: 5051 5101 5051
Size: 50 8013
Acc65I G'GTAC_C
Cuts at: 3752 5079 3752
Size: 1327 6736
AclI AA'CG_TT
Cuts at: 7903 7903
Size: 8063
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 273 691 1611 2125 3329 3495 4612 5888 6320
Size: 418 920 514 1204 166 1117 1276 432
Cuts at: 6320 7067 7335 273
Size: 747 268 1001
Fragments arranged by size:
1276 1204 1117 1001 920 747 514 432
418 268 166
AfeI AGC'GCT
Cuts at: 1563 3443 3531 1563
Size: 1880 88 6095
Fragments arranged by size:
6095 1880 88
AflIII A'CryG_T
Cuts at: 2936 3455 5415 6519 7496 2936
Size: 519 1960 1104 977 3503
Fragments arranged by size:
3503 1960 1104 977 519
AgeI A'CCGG_T
Cuts at: 6451 6451
Size: 8063
AhdI GACnn_n'nnGTC
Cuts at: 3917 3917
Size: 8063
AleI CACnn'nnGTG
Cuts at: 442 4279 442
Size: 3837 4226
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1774 2524 1774
Size: 750 7313
AlwNI CAG_nnn'CTG
Cuts at: 714 4370 4765 5368 6935 714
Size: 3656 395 603 1567 1842
Fragments arranged by size:
3656 1842 1567 603 395
ApaI G_GGCC'C
Cuts at: 4342 4661 4668 4780 4342
Size: 319 7 112 7625
Fragments arranged by size:
7625 319 112 7
ApaLI G'TGCA_C
Cuts at: 3043 6833 3043
Size: 3790 4273
ApoI r'AATT_y
Cuts at: 5067 7416 7672 5067
Size: 2349 256 5458
Fragments arranged by size:
5458 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8063
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8063
AvaI C'yCGr_G
Cuts at: 229 1531 2093 2880 3179 3409 4392 4660 5083
Size: 1302 562 787 299 230 983 268 423
Cuts at: 5083 229
Size: 3209
Fragments arranged by size:
3209 1302 983 787 562 423 299 268
230
AvrII C'CTAG_G
Cuts at: 1107 1107
Size: 8063
BamHI G'GATC_C
Cuts at: 1250 5088 1250
Size: 3838 4225
BanI G'GyrC_C
Cuts at: 721 1117 2625 3091 3598 3752 4285 4549 5079
Size: 396 1508 466 507 154 533 264 530
Cuts at: 5079 5493 5741 5776 721
Size: 414 248 35 3008
Fragments arranged by size:
3008 1508 533 530 507 466 414 396
264 248 154 35
BanII G_rGCy'C
Cuts at: 243 284 1300 1354 1610 2559 2660 2723 3653
Size: 41 1016 54 256 949 101 63 930
Cuts at: 3653 3869 4160 4342 4382 4661 4668 4780 4915
Size: 216 291 182 40 279 7 112 135
Cuts at: 4915 5077 6107 243
Size: 162 1030 2199
Fragments arranged by size:
2199 1030 1016 949 930 291 279 256
216 182 162 135 112 101 63 54
41 40 7
BbeI G_GCGC'C
Cuts at: 725 3095 4289 4553 5497 5745 725
Size: 2370 1194 264 944 248 3043
Fragments arranged by size:
3043 2370 1194 944 264 248
BbvCI CC'TCA_GC
Cuts at: 2054 4719 2054
Size: 2665 5398
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 223 257 1675 1709 223
Size: 34 1418 34 6577
Fragments arranged by size:
6577 1418 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5961 6722 8063 5961
Size: 761 1341 5961
Fragments arranged by size:
5961 1341 761
BclI T'GATC_A
Cuts at: 5583 5583
Size: 8063
BglI GCCn_nnn'nGGC
Cuts at: 418 719 827 1126 1543 1614 2752 4656 4689
Size: 301 108 299 417 71 1138 1904 33
Cuts at: 4689 4784 418
Size: 95 3697
Fragments arranged by size:
3697 1904 1138 417 301 299 108 95
71 33
BglII A'GATC_T
Cuts at: 5578 7615 5578
Size: 2037 6026
BlpI GC'TnA_GC
Cuts at: 283 622 1565 1676 5175 283
Size: 339 943 111 3499 3171
Fragments arranged by size:
3499 3171 943 339 111
Bme1580I G_kGCm'C
Cuts at: 2453 2630 3047 3601 3628 4247 4342 4661 4668
Size: 177 417 554 27 619 95 319 7
Cuts at: 4668 4780 5688 5781 6837 2453
Size: 112 908 93 1056 3679
Fragments arranged by size:
3679 1056 908 619 554 417 319 177
112 95 93 27 7
BmgBI CAC'GTC
Cuts at: 600 1392 4673 600
Size: 792 3281 3990
Fragments arranged by size:
3990 3281 792
BmrI ACTGGGnnnn_n'
Cuts at: 4154 5275 5356 5691 7835 4154
Size: 1121 81 335 2144 4382
Fragments arranged by size:
4382 2144 1121 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 555 4712 6430 555
Size: 4157 1718 2188
Fragments arranged by size:
4157 2188 1718
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 379 1578 1895 1918 2401 2590 4165 4187
Size: 147 1199 317 23 483 189 1575 22
Cuts at: 4187 4407 4660 232
Size: 220 253 3635
Fragments arranged by size:
3635 1575 1199 483 317 253 220 189
147 23 22
Bpu10I CC'TnA_GC
Cuts at: 1113 2054 2060 2230 4719 1113
Size: 941 6 170 2489 4457
Fragments arranged by size:
4457 2489 941 170 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1060 1369 3037 3853 5239 6118 6610 6908 1060
Size: 309 1668 816 1386 879 492 298 2215
Fragments arranged by size:
2215 1668 1386 879 816 492 309 298
BsaI GGTCTCn'nnnn_
Cuts at: 661 1927 2030 3961 4613 7767 661
Size: 1266 103 1931 652 3154 957
Fragments arranged by size:
3154 1931 1266 957 652 103
BsaAI yAC'GTr
Cuts at: 1803 1995 2937 3458 3684 5055 5416 1803
Size: 192 942 521 226 1371 361 4450
Fragments arranged by size:
4450 1371 942 521 361 226 192
BsaBI GATnn'nnATC
Cuts at: 6 5577 7614 6
Size: 5571 2037 455
Fragments arranged by size:
5571 2037 455
BsaHI Gr'CG_yC
Cuts at: 130 722 1178 2141 3092 3225 3248 3332 4286
Size: 592 456 963 951 133 23 84 954
Cuts at: 4286 4550 5008 5494 5742 6444 7940 130
Size: 264 458 486 248 702 1496 253
Fragments arranged by size:
1496 963 954 951 702 592 486 458
456 264 253 248 133 84 23
BsaWI w'CCGG_w
Cuts at: 5252 5773 6451 6725 6872 7283 5252
Size: 521 678 274 147 411 6032
Fragments arranged by size:
6032 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2227 2257 2596 2626 3380 3410 3541 3571 3931
Size: 30 339 30 754 30 131 30 360
Cuts at: 3931 3961 2227
Size: 30 6329
Fragments arranged by size:
6329 754 360 339 131 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 871 925 4162 4184 4390 4864 871
Size: 54 3237 22 206 474 4070
Fragments arranged by size:
4070 3237 474 206 54 22
BseYI C'CCAG_C
Cuts at: 254 675 699 1293 1645 2318 3292 3478 5011
Size: 421 24 594 352 673 974 186 1533
Cuts at: 5011 5488 6246 6823 7319 254
Size: 477 758 577 496 998
Fragments arranged by size:
1533 998 974 758 673 594 577 496
477 421 352 186 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 765 3238 3288 3354 3568 765
Size: 2473 50 66 214 5260
Fragments arranged by size:
5260 2473 214 66 50
BsiEI CG_ry'CG
Cuts at: 104 151 381 456 1055 5605 5651 6859 104
Size: 47 230 75 599 4550 46 1208 1308
Fragments arranged by size:
4550 1308 1208 599 230 75 47 46
BsiHKAI G_wGCw'C
Cuts at: 284 628 863 2723 3047 5077 5855 6045 6837
Size: 344 235 1860 324 2030 778 190 792
Cuts at: 6837 284
Size: 1510
Fragments arranged by size:
2030 1860 1510 792 778 344 324 235
190
BsmBI CGTCTCn'nnnn_
Cuts at: 2092 2581 3232 2092
Size: 489 651 6923
Fragments arranged by size:
6923 651 489
Bsp1286I G_dGCh'C
Cuts at: 243 284 628 863 1300 1354 1610 2453 2559
Size: 41 344 235 437 54 256 843 106
Cuts at: 2559 2630 2660 2723 3047 3601 3628 3653 3869
Size: 71 30 63 324 554 27 25 216
Cuts at: 3869 4160 4247 4342 4382 4661 4668 4780 4915
Size: 291 87 95 40 279 7 112 135
Cuts at: 4915 5077 5688 5781 5855 6045 6107 6837 243
Size: 162 611 93 74 190 62 730 1469
Fragments arranged by size:
1469 843 730 611 554 437 344 324
291 279 256 235 216 190 162 135
112 106 95 93 87 74 71 63
62 54 41 40 30 27 25 7
BspEI T'CCGG_A
Cuts at: 7283 7283
Size: 8063
BspHI T'CATG_A
Cuts at: 2336 2597 7239 2336
Size: 261 4642 3160
Fragments arranged by size:
4642 3160 261
BspMI ACCTGCnnnn'nnnn_
Cuts at: 549 1205 1352 1955 3167 3258 3310 3370 3584
Size: 656 147 603 1212 91 52 60 214
Cuts at: 3584 3714 3932 4109 4601 5113 5327 5629 6010
Size: 130 218 177 492 512 214 302 381
Cuts at: 6010 549
Size: 2602
Fragments arranged by size:
2602 1212 656 603 512 492 381 302
218 214 214 177 147 130 91 60
52
BsrBI CCG'CTC
Cuts at: 50 684 2607 3750 4058 4356 4513 4773 6355
Size: 634 1923 1143 308 298 157 260 1582
Cuts at: 6355 6409 7893 8058 50
Size: 54 1484 165 55
Fragments arranged by size:
1923 1582 1484 1143 634 308 298 260
165 157 55 54
BsrDI GCAATG_nn'
Cuts at: 942 2139 2898 3408 4971 5975 942
Size: 1197 759 510 1563 1004 3030
Fragments arranged by size:
3030 1563 1197 1004 759 510
BsrFI r'CCGG_y
Cuts at: 140 417 441 1637 2188 3738 4596 6061 6242
Size: 277 24 1196 551 1550 858 1465 181
Cuts at: 6242 6451 140
Size: 209 1752
Fragments arranged by size:
1752 1550 1465 1196 858 551 277 209
181 24
BsrGI T'GTAC_A
Cuts at: 2249 3122 2249
Size: 873 7190
BssHII G'CGCG_C
Cuts at: 196 198 250 312 314 487 743 751 4318
Size: 2 52 62 2 173 256 8 3567
Cuts at: 4318 196
Size: 3941
Fragments arranged by size:
3941 3567 256 173 62 52 8 2
2
BssSI C'ACGA_G
Cuts at: 2659 3483 6334 6692 2659
Size: 824 2851 358 4030
Fragments arranged by size:
4030 2851 824 358
BstAPI GCAn_nnn'nTGC
Cuts at: 468 761 845 3302 5440 468
Size: 293 84 2457 2138 3091
Fragments arranged by size:
3091 2457 2138 293 84
BstBI TT'CG_AA
Cuts at: 110 6424 110
Size: 6314 1749
BstEII G'GTnAC_C
Cuts at: 1185 1230 3919 1185
Size: 45 2689 5329
Fragments arranged by size:
5329 2689 45
BstXI CCAn_nnnn'nTGG
Cuts at: 1242 1819 1967 3171 3987 4011 4029 4827 4966
Size: 577 148 1204 816 24 18 798 139
Cuts at: 4966 1242
Size: 4339
Fragments arranged by size:
4339 1204 816 798 577 148 139 24
18
BstYI r'GATC_y
Cuts at: 1 1250 1741 5088 5555 5578 5913 6159 7171
Size: 1249 491 3347 467 23 335 246 1012
Cuts at: 7171 7257 7269 7615 1
Size: 86 12 346 449
Fragments arranged by size:
3347 1249 1012 491 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1928 4528 1928
Size: 2600 5463
BtgI C'CryG_G
Cuts at: 125 651 866 1697 2222 2621 2849 3503 3602
Size: 526 215 831 525 399 228 654 99
Cuts at: 3602 3971 4028 4220 4436 4544 4684 4820 125
Size: 369 57 192 216 108 140 136 3368
Fragments arranged by size:
3368 831 654 526 525 399 369 228
216 215 192 140 136 108 99 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3225 4145 6013 6194 6357 3225
Size: 920 1868 181 163 4931
Fragments arranged by size:
4931 1868 920 181 163
BtsI GCAGTG_nn'
Cuts at: 241 2116 3352 4796 5306 241
Size: 1875 1236 1444 510 2998
Fragments arranged by size:
2998 1875 1444 1236 510
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4659 4694 4659
Size: 35 8028
DraI TTT'AAA
Cuts at: 5062 5062
Size: 8063
DraIII CAC_nnn'GTG
Cuts at: 468 2481 2937 3015 3687 468
Size: 2013 456 78 672 4844
Fragments arranged by size:
4844 2013 672 456 78
DrdI GACnn_nn'nnGTC
Cuts at: 1183 2902 2989 5769 6627 1183
Size: 1719 87 2780 858 2619
Fragments arranged by size:
2780 2619 1719 858 87
EaeI y'GGCC_r
Cuts at: 148 378 419 446 563 662 1052 1713 1868
Size: 230 41 27 117 99 390 661 155
Cuts at: 1868 2342 2670 2753 3500 3736 3884 3974 3998
Size: 474 328 83 747 236 148 90 24
Cuts at: 3998 4031 4223 5648 5822 6213 6240 7585 148
Size: 33 192 1425 174 391 27 1345 626
Fragments arranged by size:
1425 1345 747 661 626 474 391 390
328 236 230 192 174 155 148 117
99 90 83 41 33 27 27 24
EagI C'GGCC_G
Cuts at: 148 378 1052 5648 148
Size: 230 674 4596 2563
Fragments arranged by size:
4596 2563 674 230
EarI CTCTTCn'nnn_
Cuts at: 848 1630 2020 2836 3305 6086 6296 848
Size: 782 390 816 469 2781 210 2615
Fragments arranged by size:
2781 2615 816 782 469 390 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4566 6271 6581 6727 7873 4566
Size: 1705 310 146 1146 4756
Fragments arranged by size:
4756 1705 1146 310 146
EcoICRI GAG'CTC
Cuts at: 282 2721 5075 282
Size: 2439 2354 3270
Fragments arranged by size:
3270 2439 2354
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 273 379 691 1578 1611 1895 1918 2125
Size: 41 106 312 887 33 284 23 207
Cuts at: 2125 2401 2590 3329 3495 4165 4187 4407 4612
Size: 276 189 739 166 670 22 220 205
Cuts at: 4612 4660 5888 6320 7067 7335 232
Size: 48 1228 432 747 268 960
Fragments arranged by size:
1228 960 887 747 739 670 432 312
284 276 268 220 207 205 189 166
106 48 41 33 23 22
EcoNI CCTnn'n_nnAGG
Cuts at: 1438 2053 1438
Size: 615 7448
EcoO109I rG'GnC_Cy
Cuts at: 711 1128 1449 2010 2046 2428 3030 3391 4140
Size: 417 321 561 36 382 602 361 749
Cuts at: 4140 4338 4657 4664 4665 4691 4762 4776 4989
Size: 198 319 7 1 26 71 14 213
Cuts at: 4989 5202 711
Size: 213 3572
Fragments arranged by size:
3572 749 602 561 417 382 361 321
319 213 213 198 71 36 26 14
7 1
EcoRI G'AATT_C
Cuts at: 5067 5067
Size: 8063
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6082 6082
Size: 8063
FspI TGC'GCA
Cuts at: 298 5844 298
Size: 5546 2517
HaeII r_GCGC'y
Cuts at: 190 502 616 725 1549 1565 3095 3445 3533
Size: 312 114 109 824 16 1530 350 88
Cuts at: 3533 3557 3883 4289 4553 4858 5497 5745 6767
Size: 24 326 406 264 305 639 248 1022
Cuts at: 6767 7746 190
Size: 979 507
Fragments arranged by size:
1530 1022 979 824 639 507 406 350
326 312 305 264 248 114 109 88
24 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1726 1758 2284 2316 3380 3412 3431 3463 3477
Size: 32 526 32 1064 32 19 32 14
Cuts at: 3477 3509 3541 3573 3903 3931 3935 3963 4897
Size: 32 32 32 330 28 4 28 934
Cuts at: 4897 4929 5905 5935 5937 5967 1726
Size: 32 976 30 2 30 3822
Fragments arranged by size:
3822 1064 976 934 526 330 32 32
32 32 32 32 32 32 30 30
28 28 19 14 4 2
HincII GTy'rAC
Cuts at: 1065 2415 3744 5102 1065
Size: 1350 1329 1358 4026
Fragments arranged by size:
4026 1358 1350 1329
KasI G'GCGC_C
Cuts at: 721 3091 4285 4549 5493 5741 721
Size: 2370 1194 264 944 248 3043
Fragments arranged by size:
3043 2370 1194 944 264 248
KpnI G_GTAC'C
Cuts at: 3756 5083 3756
Size: 1327 6736
MluI A'CGCG_T
Cuts at: 7496 7496
Size: 8063
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2657 6734 6918 2657
Size: 4077 184 3802
Fragments arranged by size:
4077 3802 184
MscI TGG'CCA
Cuts at: 565 664 1870 2344 2672 4000 4033 4225 5824
Size: 99 1206 474 328 1328 33 192 1599
Cuts at: 5824 565
Size: 2804
Fragments arranged by size:
2804 1599 1328 1206 474 328 192 99
33
MslI CAynn'nnrTG
Cuts at: 442 1240 1840 2251 2651 3086 3487 4009 4027
Size: 798 600 411 400 435 401 522 18
Cuts at: 4027 4279 4825 4831 6052 6179 442
Size: 252 546 6 1221 127 2326
Fragments arranged by size:
2326 1221 798 600 546 522 435 411
401 400 252 127 18 6
MspA1I CmG'CkG
Cuts at: 165 275 432 458 653 738 1339 1487 1556
Size: 110 157 26 195 85 601 148 69
Cuts at: 1556 1680 2322 2514 3525 3582 3734 3771 4686
Size: 124 642 192 1011 57 152 37 915
Cuts at: 4686 5174 5488 5848 6861 7106 165
Size: 488 314 360 1013 245 1122
Fragments arranged by size:
1122 1013 1011 915 642 601 488 360
314 245 195 192 157 152 148 124
110 85 69 57 37 26
NaeI GCC'GGC
Cuts at: 419 1639 4598 6244 419
Size: 1220 2959 1646 2238
Fragments arranged by size:
2959 2238 1646 1220
NarI GG'CG_CC
Cuts at: 722 3092 4286 4550 5494 5742 722
Size: 2370 1194 264 944 248 3043
Fragments arranged by size:
3043 2370 1194 944 264 248
NcoI C'CATG_G
Cuts at: 125 866 1697 4028 125
Size: 741 831 2331 4160
Fragments arranged by size:
4160 2331 831 741
NdeI CA'TA_TG
Cuts at: 2561 2561
Size: 8063
NgoMIV G'CCGG_C
Cuts at: 417 1637 4596 6242 417
Size: 1220 2959 1646 2238
Fragments arranged by size:
2959 2238 1646 1220
NspI r_CATG'y
Cuts at: 5116 5616 6523 5116
Size: 500 907 6656
Fragments arranged by size:
6656 907 500
PasI CC'CwG_GG
Cuts at: 4555 4555
Size: 8063
PciI A'CATG_T
Cuts at: 6519 6519
Size: 8063
PfoI T'CCnGG_A
Cuts at: 133 356 4802 133
Size: 223 4446 3394
Fragments arranged by size:
4446 3394 223
PmeI GTTT'AAAC
Cuts at: 5062 5062
Size: 8063
PmlI CAC'GTG
Cuts at: 1803 3458 3684 1803
Size: 1655 226 6182
Fragments arranged by size:
6182 1655 226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7202 7234 7202
Size: 32 8031
PpuMI rG'GwC_Cy
Cuts at: 1449 2010 3030 4140 1449
Size: 561 1020 1110 5372
Fragments arranged by size:
5372 1110 1020 561
PspOMI G'GGCC_C
Cuts at: 4338 4657 4664 4776 4338
Size: 319 7 112 7625
Fragments arranged by size:
7625 319 112 7
PstI C_TGCA'G
Cuts at: 431 820 850 1469 2513 3581 3725 5110 5795
Size: 389 30 619 1044 1068 144 1385 685
Cuts at: 5795 431
Size: 2699
Fragments arranged by size:
2699 1385 1068 1044 685 619 389 144
30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8063
PvuII CAG'CTG
Cuts at: 432 1339 2514 3582 3734 3771 5488 5848 432
Size: 907 1175 1068 152 37 1717 360 2647
Fragments arranged by size:
2647 1717 1175 1068 907 360 152 37
RsrII CG'GwC_CG
Cuts at: 4479 6258 4479
Size: 1779 6284
SacI G_AGCT'C
Cuts at: 284 2723 5077 284
Size: 2439 2354 3270
Fragments arranged by size:
3270 2439 2354
SacII CC_GC'GG
Cuts at: 654 4687 654
Size: 4033 4030
SalI G'TCGA_C
Cuts at: 5100 5100
Size: 8063
SanDI GG'GwC_CC
Cuts at: 1449 3030 1449
Size: 1581 6482
SapI GCTCTTCn'nnn_
Cuts at: 1630 6086 6296 1630
Size: 4456 210 3397
Fragments arranged by size:
4456 3397 210
SbfI CC_TGCA'GG
Cuts at: 3725 5110 3725
Size: 1385 6678
ScaI AGT'ACT
Cuts at: 4164 7580 4164
Size: 3416 4647
SexAI A'CCwGG_T
Cuts at: 4919 4919
Size: 8063
SfcI C'TryA_G
Cuts at: 33 427 816 846 1465 2509 3577 3721 5106
Size: 394 389 30 619 1044 1068 144 1385
Cuts at: 5106 5791 6784 6975 33
Size: 685 993 191 1121
Fragments arranged by size:
1385 1121 1068 1044 993 685 619 394
389 191 144 30
SfoI GGC'GCC
Cuts at: 723 3093 4287 4551 5495 5743 723
Size: 2370 1194 264 944 248 3043
Fragments arranged by size:
3043 2370 1194 944 264 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8063
SgrAI Cr'CCGG_yG
Cuts at: 441 441
Size: 8063
SmaI CCC'GGG
Cuts at: 231 2095 3181 3411 4662 5085 231
Size: 1864 1086 230 1251 423 3209
Fragments arranged by size:
3209 1864 1251 1086 423 230
SmlI C'TyrA_G
Cuts at: 1075 1384 3052 3868 5218 6133 6625 6887 1075
Size: 309 1668 816 1350 915 492 262 2251
Fragments arranged by size:
2251 1668 1350 915 816 492 309 262
SnaBI TAC'GTA
Cuts at: 5055 5055
Size: 8063
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8063
SphI G_CATG'C
Cuts at: 5116 5616 5116
Size: 500 7563
SspI AAT'ATT
Cuts at: 4181 4181
Size: 8063
StuI AGG'CCT
Cuts at: 1537 4390 1537
Size: 2853 5210
StyI C'CwwG_G
Cuts at: 125 866 1107 1697 3564 4028 4652 5197 5551
Size: 741 241 590 1867 464 624 545 354
Cuts at: 5551 125
Size: 2637
Fragments arranged by size:
2637 1867 741 624 590 545 464 354
241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3532 4099 4306 5646 5779 6238 6447 3532
Size: 567 207 1340 133 459 209 5148
Fragments arranged by size:
5148 1340 567 459 209 207 133
TatI w'GTAC_w
Cuts at: 2249 3122 4162 7578 2249
Size: 873 1040 3416 2734
Fragments arranged by size:
3416 2734 1040 873
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 989 1021 4449 4481 7580 7612 989
Size: 32 3428 32 3099 32 1440
Fragments arranged by size:
3428 3099 1440 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1060 1393 5860 1060
Size: 333 4467 3263
Fragments arranged by size:
4467 3263 333
XbaI T'CTAG_A
Cuts at: 5094 5094
Size: 8063
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 429 672 1704 2695 3486 3723 3984 4008 4467
Size: 243 1032 991 791 237 261 24 459
Cuts at: 4467 4827 5558 429
Size: 360 731 2934
Fragments arranged by size:
2934 1032 991 791 731 459 360 261
243 237 24
XmaI C'CCGG_G
Cuts at: 229 2093 3179 3409 4660 5083 229
Size: 1864 1086 230 1251 423 3209
Fragments arranged by size:
3209 1864 1251 1086 423 230
XmnI GAAnn'nnTTC
Cuts at: 2164 3148 2164
Size: 984 7079
ZraI GAC'GTC
Cuts at: 1179 3226 1179
Size: 2047 6016
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AloI AscI BaeI BbsI BfrBI BmtI BsiWI
BsmI BstZ17I ClaI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PflMI PshAI
PsiI PspXI PsrI SfiI SrfI SwaI XhoI