Restriction map for
pF1KSDA0915
(Circular) (Six-base) MAPSORT of: pF1KSDA0915.seq Check: 1373 from: 1 to: 5662
pF1KSDA0915 5662 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2439 2489 2439
Size: 50 5612
AatII G_ACGT'C
Cuts at: 1408 1408
Size: 5662
AccI GT'mk_AC
Cuts at: 2698 2698
Size: 5662
Acc65I G'GTAC_C
Cuts at: 2676 2676
Size: 5662
AclI AA'CG_TT
Cuts at: 5502 5502
Size: 5662
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 629 1061 1159 1970 2462 2516 2527 2606 3485
Size: 432 98 811 492 54 11 79 879
Cuts at: 3485 3917 4666 4934 629
Size: 432 749 268 1357
Fragments arranged by size:
1357 879 811 749 492 432 432 268
98 79 54 11
AfeI AGC'GCT
Cuts at: 1923 2326 1923
Size: 403 5259
AflIII A'CryG_T
Cuts at: 3012 4118 5095 3012
Size: 1106 977 3579
Fragments arranged by size:
3579 1106 977
AgeI A'CCGG_T
Cuts at: 4050 4050
Size: 5662
AleI CACnn'nnGTG
Cuts at: 1453 1708 1453
Size: 255 5407
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 532 964 1840 532
Size: 432 876 4354
Fragments arranged by size:
4354 876 432
AlwNI CAG_nnn'CTG
Cuts at: 460 565 730 1102 2178 2409 2965 4534 460
Size: 105 165 372 1076 231 556 1569 1588
Fragments arranged by size:
1588 1569 1076 556 372 231 165 105
ApaLI G'TGCA_C
Cuts at: 297 4432 297
Size: 4135 1527
ApoI r'AATT_y
Cuts at: 2032 2664 5015 5271 2032
Size: 632 2351 256 2423
Fragments arranged by size:
2423 2351 632 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5662
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5662
AvaI C'yCGr_G
Cuts at: 2602 2680 2602
Size: 78 5584
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1165 1198 1165
Size: 33 5629
BamHI G'GATC_C
Cuts at: 2223 2685 2223
Size: 462 5200
BanI G'GyrC_C
Cuts at: 801 2024 2060 2576 2626 2676 3090 3338 3373
Size: 1223 36 516 50 50 414 248 35
Cuts at: 3373 801
Size: 3090
Fragments arranged by size:
3090 1223 516 414 248 50 50 36
35
BanII G_rGCy'C
Cuts at: 320 621 794 2674 3704 320
Size: 301 173 1880 1030 2278
Fragments arranged by size:
2278 1880 1030 301 173
BbeI G_GCGC'C
Cuts at: 2028 3094 3342 2028
Size: 1066 248 4348
Fragments arranged by size:
4348 1066 248
BbsI GAAGACnn'nnnn_
Cuts at: 1336 1336
Size: 5662
BbvCI CC'TCA_GC
Cuts at: 360 558 1357 1925 360
Size: 198 799 568 4097
Fragments arranged by size:
4097 799 568 198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1890 1924 1890
Size: 34 5628
BciVI GTATCCnnnnn_n'
Cuts at: 1344 3558 4321 5662 1344
Size: 2214 763 1341 1344
Fragments arranged by size:
2214 1344 1341 763
BclI T'GATC_A
Cuts at: 1494 3180 1494
Size: 1686 3976
BglI GCCn_nnn'nGGC
Cuts at: 1882 1882
Size: 5662
BglII A'GATC_T
Cuts at: 1023 3175 5214 1023
Size: 2152 2039 1471
Fragments arranged by size:
2152 2039 1471
BlpI GC'TnA_GC
Cuts at: 935 1459 2772 935
Size: 524 1313 3825
Fragments arranged by size:
3825 1313 524
Bme1580I G_kGCm'C
Cuts at: 301 3285 3378 4436 301
Size: 2984 93 1058 1527
Fragments arranged by size:
2984 1527 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 129 213 2872 2953 3288 5434 129
Size: 84 2659 81 335 2146 357
Fragments arranged by size:
2659 2146 357 335 84 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 394 701 1265 4027 394
Size: 307 564 2762 2029
Fragments arranged by size:
2762 2029 564 307
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 551 707 808 1079 1235 1283 2476 458
Size: 93 156 101 271 156 48 1193 3644
Fragments arranged by size:
3644 1193 271 156 156 101 93 48
Bpu10I CC'TnA_GC
Cuts at: 360 558 620 1357 1925 360
Size: 198 62 737 568 4097
Fragments arranged by size:
4097 737 568 198 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 943 2836 3715 4209 4507 319
Size: 624 1893 879 494 298 1474
Fragments arranged by size:
1893 1474 879 624 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 476 652 1675 2016 5366 476
Size: 176 1023 341 3350 772
Fragments arranged by size:
3350 1023 772 341 176
BsaAI yAC'GTr
Cuts at: 2652 3013 2652
Size: 361 5301
BsaBI GATnn'nnATC
Cuts at: 6 1028 3174 5213 6
Size: 1022 2146 2039 455
Fragments arranged by size:
2146 2039 1022 455
BsaHI Gr'CG_yC
Cuts at: 1405 2025 3091 3339 4043 5539 1405
Size: 620 1066 248 704 1496 1528
Fragments arranged by size:
1528 1496 1066 704 620 248
BsaWI w'CCGG_w
Cuts at: 2220 2849 3370 4050 4324 4471 4882 2220
Size: 629 521 680 274 147 411 3000
Fragments arranged by size:
3000 680 629 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 229 259 1411 1441 229
Size: 30 1152 30 4450
Fragments arranged by size:
4450 1152 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 237 835 946 1070 1082 1135 1228 1693 2586
Size: 598 111 124 12 53 93 465 893
Cuts at: 2586 237
Size: 3313
Fragments arranged by size:
3313 893 598 465 124 111 93 53
12
BseYI C'CCAG_C
Cuts at: 321 2210 2331 2534 3085 3843 4422 4918 321
Size: 1889 121 203 551 758 579 496 1065
Fragments arranged by size:
1889 1065 758 579 551 496 203 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 492 1540 2411 2467 492
Size: 1048 871 56 3687
Fragments arranged by size:
3687 1048 871 56
BsiEI CG_ry'CG
Cuts at: 104 3202 3248 4458 104
Size: 3098 46 1210 1308
Fragments arranged by size:
3098 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 301 723 794 2081 2674 3452 3642 4436 301
Size: 422 71 1287 593 778 190 794 1527
Fragments arranged by size:
1527 1287 794 778 593 422 190 71
BsmI GAATG_Cn'
Cuts at: 1878 1878
Size: 5662
Bsp1286I G_dGCh'C
Cuts at: 301 320 621 723 794 2081 2674 3285 3378
Size: 19 301 102 71 1287 593 611 93
Cuts at: 3378 3452 3642 3704 4436 301
Size: 74 190 62 732 1527
Fragments arranged by size:
1527 1287 732 611 593 301 190 102
93 74 71 62 19
BspEI T'CCGG_A
Cuts at: 2220 4882 2220
Size: 2662 3000
BspHI T'CATG_A
Cuts at: 4838 4838
Size: 5662
BspMI ACCTGCnnnn'nnnn_
Cuts at: 468 581 1430 2141 2205 2439 2489 2710 2924
Size: 113 849 711 64 234 50 221 214
Cuts at: 2924 3226 3607 468
Size: 302 381 2523
Fragments arranged by size:
2523 849 711 381 302 234 221 214
113 64 50
BsrBI CCG'CTC
Cuts at: 50 926 1545 1761 3952 4006 5492 5657 50
Size: 876 619 216 2191 54 1486 165 55
Fragments arranged by size:
2191 1486 876 619 216 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3572 3572
Size: 5662
BsrFI r'CCGG_y
Cuts at: 1733 3658 3839 4050 1733
Size: 1925 181 211 3345
Fragments arranged by size:
3345 1925 211 181
BssSI C'ACGA_G
Cuts at: 977 3931 4291 977
Size: 2954 360 2348
Fragments arranged by size:
2954 2348 360
BstAPI GCAn_nnn'nTGC
Cuts at: 3037 3037
Size: 5662
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5662
BstEII G'GTnAC_C
Cuts at: 500 895 500
Size: 395 5267
BstYI r'GATC_y
Cuts at: 1 1023 2223 2685 3152 3175 3510 3756 4770
Size: 1022 1200 462 467 23 335 246 1014
Cuts at: 4770 4856 4868 5214 1
Size: 86 12 346 449
Fragments arranged by size:
1200 1022 1014 467 462 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 524 524
Size: 5662
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2176 3610 3791 3954 2176
Size: 1434 181 163 3884
Fragments arranged by size:
3884 1434 181 163
BtsI GCAGTG_nn'
Cuts at: 929 2903 929
Size: 1974 3688
DraI TTT'AAA
Cuts at: 2659 2659
Size: 5662
DraIII CAC_nnn'GTG
Cuts at: 1310 1626 1310
Size: 316 5346
DrdI GACnn_nn'nnGTC
Cuts at: 3366 4226 3366
Size: 860 4802
EaeI y'GGCC_r
Cuts at: 3245 3419 3810 3837 5184 3245
Size: 174 391 27 1347 3723
Fragments arranged by size:
3723 1347 391 174 27
EagI C'GGCC_G
Cuts at: 3245 3245
Size: 5662
EarI CTCTTCn'nnn_
Cuts at: 1112 1513 1584 1844 1946 3683 3893 1112
Size: 401 71 260 102 1737 210 2881
Fragments arranged by size:
2881 1737 401 260 210 102 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 175 435 1710 2236 3868 4180 4326 5472 175
Size: 260 1275 526 1632 312 146 1146 365
Fragments arranged by size:
1632 1275 1146 526 365 312 260 146
EcoICRI GAG'CTC
Cuts at: 792 2672 792
Size: 1880 3782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 551 629 707 808 1061 1079 1159 1235
Size: 93 78 78 101 253 18 80 76
Cuts at: 1235 1283 1970 2462 2476 2516 2527 2606 3485
Size: 48 687 492 14 40 11 79 879
Cuts at: 3485 3917 4666 4934 458
Size: 432 749 268 1186
Fragments arranged by size:
1186 879 749 687 492 432 268 253
101 93 80 79 78 78 76 48
40 18 14 11
EcoNI CCTnn'n_nnAGG
Cuts at: 1312 2203 2621 1312
Size: 891 418 4353
Fragments arranged by size:
4353 891 418
EcoO109I rG'GnC_Cy
Cuts at: 229 704 825 834 1008 1630 1884 1998 2353
Size: 475 121 9 174 622 254 114 355
Cuts at: 2353 2522 2799 229
Size: 169 277 3092
Fragments arranged by size:
3092 622 475 355 277 254 174 169
121 114 9
EcoRI G'AATT_C
Cuts at: 2032 2664 2032
Size: 632 5030
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3679 3679
Size: 5662
FspI TGC'GCA
Cuts at: 1816 3441 1816
Size: 1625 4037
HaeII r_GCGC'y
Cuts at: 444 1925 2028 2328 3094 3342 4366 5345 444
Size: 1481 103 300 766 248 1024 979 761
Fragments arranged by size:
1481 1024 979 766 761 300 248 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3502 3532 3534 3564 3502
Size: 30 2 30 5600
Fragments arranged by size:
5600 30 30 2
HincII GTy'rAC
Cuts at: 2699 2699
Size: 5662
HindIII A'AGCT_T
Cuts at: 2300 2300
Size: 5662
KasI G'GCGC_C
Cuts at: 2024 3090 3338 2024
Size: 1066 248 4348
Fragments arranged by size:
4348 1066 248
KpnI G_GTAC'C
Cuts at: 2680 2680
Size: 5662
MluI A'CGCG_T
Cuts at: 5095 5095
Size: 5662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2592 4333 4517 2592
Size: 1741 184 3737
Fragments arranged by size:
3737 1741 184
MscI TGG'CCA
Cuts at: 3421 3421
Size: 5662
MslI CAynn'nnrTG
Cuts at: 1453 1708 3649 3776 1453
Size: 255 1941 127 3339
Fragments arranged by size:
3339 1941 255 127
MspA1I CmG'CkG
Cuts at: 325 855 1340 1770 2166 2538 2771 3085 3445
Size: 530 485 430 396 372 233 314 360
Cuts at: 3445 4460 4705 325
Size: 1015 245 1282
Fragments arranged by size:
1282 1015 530 485 430 396 372 360
314 245 233
NaeI GCC'GGC
Cuts at: 1735 3841 1735
Size: 2106 3556
NarI GG'CG_CC
Cuts at: 2025 3091 3339 2025
Size: 1066 248 4348
Fragments arranged by size:
4348 1066 248
NcoI C'CATG_G
Cuts at: 524 524
Size: 5662
NgoMIV G'CCGG_C
Cuts at: 1733 3839 1733
Size: 2106 3556
NruI TCG'CGA
Cuts at: 4023 4023
Size: 5662
NspI r_CATG'y
Cuts at: 1380 1618 1821 2322 2713 3213 4122 1380
Size: 238 203 501 391 500 909 2920
Fragments arranged by size:
2920 909 501 500 391 238 203
PasI CC'CwG_GG
Cuts at: 226 830 1887 226
Size: 604 1057 4001
Fragments arranged by size:
4001 1057 604
PciI A'CATG_T
Cuts at: 4118 4118
Size: 5662
PflMI CCAn_nnn'nTGG
Cuts at: 311 730 1626 311
Size: 419 896 4347
Fragments arranged by size:
4347 896 419
PfoI T'CCnGG_A
Cuts at: 1472 1472
Size: 5662
PmeI GTTT'AAAC
Cuts at: 2659 2659
Size: 5662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4801 4833 4801
Size: 32 5630
PpuMI rG'GwC_Cy
Cuts at: 704 825 834 1008 704
Size: 121 9 174 5358
Fragments arranged by size:
5358 174 121 9
PstI C_TGCA'G
Cuts at: 150 1169 1742 1829 2707 3392 150
Size: 1019 573 87 878 685 2420
Fragments arranged by size:
2420 1019 878 685 573 87
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5662
PvuII CAG'CTG
Cuts at: 325 2538 3085 3445 325
Size: 2213 547 360 2542
Fragments arranged by size:
2542 2213 547 360
RsrII CG'GwC_CG
Cuts at: 3855 3855
Size: 5662
SacI G_AGCT'C
Cuts at: 794 2674 794
Size: 1880 3782
SalI G'TCGA_C
Cuts at: 2697 2697
Size: 5662
SanDI GG'GwC_CC
Cuts at: 704 834 1008 704
Size: 130 174 5358
Fragments arranged by size:
5358 174 130
SapI GCTCTTCn'nnn_
Cuts at: 1584 1946 3683 3893 1584
Size: 362 1737 210 3353
Fragments arranged by size:
3353 1737 362 210
SbfI CC_TGCA'GG
Cuts at: 1169 2707 1169
Size: 1538 4124
ScaI AGT'ACT
Cuts at: 5179 5179
Size: 5662
SfcI C'TryA_G
Cuts at: 33 146 1165 1686 1738 1825 2703 3388 4383
Size: 113 1019 521 52 87 878 685 995
Cuts at: 4383 4574 33
Size: 191 1121
Fragments arranged by size:
1121 1019 995 878 685 521 191 113
87 52
SfoI GGC'GCC
Cuts at: 2026 3092 3340 2026
Size: 1066 248 4348
Fragments arranged by size:
4348 1066 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5662
SmaI CCC'GGG
Cuts at: 2682 2682
Size: 5662
SmlI C'TyrA_G
Cuts at: 334 958 2602 2815 3730 4224 4486 334
Size: 624 1644 213 915 494 262 1510
Fragments arranged by size:
1644 1510 915 624 494 262 213
SnaBI TAC'GTA
Cuts at: 2652 2652
Size: 5662
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5662
SphI G_CATG'C
Cuts at: 1380 1618 1821 2713 3213 1380
Size: 238 203 892 500 3829
Fragments arranged by size:
3829 892 500 238 203
StyI C'CwwG_G
Cuts at: 524 2794 3148 524
Size: 2270 354 3038
Fragments arranged by size:
3038 2270 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 501 789 883 1219 1287 1459 1879 1984 2299
Size: 288 94 336 68 172 420 105 315
Cuts at: 2299 3243 3376 3835 4046 501
Size: 944 133 459 211 2117
Fragments arranged by size:
2117 944 459 420 336 315 288 211
172 133 105 94 68
TatI w'GTAC_w
Cuts at: 5177 5177
Size: 5662
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 229 261 411 443 1262 1294 5179 5211 229
Size: 32 150 32 819 32 3885 32 680
Fragments arranged by size:
3885 819 680 150 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2450 3457 2450
Size: 1007 4655
XbaI T'CTAG_A
Cuts at: 367 2691 367
Size: 2324 3338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1344 3155 1344
Size: 1811 3851
XhoI C'TCGA_G
Cuts at: 2602 2602
Size: 5662
XmaI C'CCGG_G
Cuts at: 2680 2680
Size: 5662
ZraI GAC'GTC
Cuts at: 1406 1406
Size: 5662
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
AflII AhdI AloI ApaI AscI AvrII BfrBI BmgBI
BmtI BsiWI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NsiI PacI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SrfI SspI
StuI SwaI XmnI