Restriction map for
pF1KSDA0888
(Circular) (Six-base) MAPSORT of: pF1KSDA0888.seq Check: 6624 from: 1 to: 5149
pF1KSDA0888 5149 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 432 432
Size: 5149
AccI GT'mk_AC
Cuts at: 790 1237 1599 2066 2185 790
Size: 447 362 467 119 3754
Fragments arranged by size:
3754 467 447 362 119
Acc65I G'GTAC_C
Cuts at: 869 2163 869
Size: 1294 3855
AclI AA'CG_TT
Cuts at: 4989 4989
Size: 5149
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 362 1025 1991 2972 3404 4153 4421 206
Size: 156 663 966 981 432 749 268 934
Fragments arranged by size:
981 966 934 749 663 432 268 156
AflII C'TTAA_G
Cuts at: 436 1432 436
Size: 996 4153
AflIII A'CryG_T
Cuts at: 194 2499 3605 4582 194
Size: 2305 1106 977 761
Fragments arranged by size:
2305 1106 977 761
AgeI A'CCGG_T
Cuts at: 1788 3537 1788
Size: 1749 3400
AhdI GACnn_n'nnGTC
Cuts at: 1014 1014
Size: 5149
AlwNI CAG_nnn'CTG
Cuts at: 2452 4021 2452
Size: 1569 3580
ApaLI G'TGCA_C
Cuts at: 3919 3919
Size: 5149
ApoI r'AATT_y
Cuts at: 177 320 917 1162 1267 1303 1774 1800 2076
Size: 143 597 245 105 36 471 26 276
Cuts at: 2076 2151 4502 4758 177
Size: 75 2351 256 568
Fragments arranged by size:
2351 597 568 471 276 256 245 143
105 75 36 26
AseI AT'TA_AT
Cuts at: 20 20
Size: 5149
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5149
AvaI C'yCGr_G
Cuts at: 2167 2167
Size: 5149
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 853 886 853
Size: 33 5116
BamHI G'GATC_C
Cuts at: 2172 2172
Size: 5149
BanI G'GyrC_C
Cuts at: 869 2163 2577 2825 2860 869
Size: 1294 414 248 35 3158
Fragments arranged by size:
3158 1294 414 248 35
BanII G_rGCy'C
Cuts at: 1261 1862 2161 3191 1261
Size: 601 299 1030 3219
Fragments arranged by size:
3219 1030 601 299
BbeI G_GCGC'C
Cuts at: 2581 2829 2581
Size: 248 4901
BbsI GAAGACnn'nnnn_
Cuts at: 770 1013 1466 1526 1838 1922 770
Size: 243 453 60 312 84 3997
Fragments arranged by size:
3997 453 312 243 84 60
BciVI GTATCCnnnnn_n'
Cuts at: 779 830 3045 3808 5149 779
Size: 51 2215 763 1341 779
Fragments arranged by size:
2215 1341 779 763 51
BclI T'GATC_A
Cuts at: 378 2667 378
Size: 2289 2860
BglII A'GATC_T
Cuts at: 2662 4701 2662
Size: 2039 3110
BlpI GC'TnA_GC
Cuts at: 2126 2259 2126
Size: 133 5016
Bme1580I G_kGCm'C
Cuts at: 1652 2772 2865 3923 1652
Size: 1120 93 1058 2878
Fragments arranged by size:
2878 1120 1058 93
BmgBI CAC'GTC
Cuts at: 1920 1920
Size: 5149
BmrI ACTGGGnnnn_n'
Cuts at: 2359 2440 2775 4921 2359
Size: 81 335 2146 2587
Fragments arranged by size:
2587 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1479 3514 1479
Size: 2035 3114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 980 742
Size: 238 4911
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1160 2323 3202 3696 3994 1160
Size: 1163 879 494 298 2315
Fragments arranged by size:
2315 1163 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 821 1797 4853 821
Size: 976 3056 1117
Fragments arranged by size:
3056 1117 976
BsaAI yAC'GTr
Cuts at: 1597 2139 2500 1597
Size: 542 361 4246
Fragments arranged by size:
4246 542 361
BsaBI GATnn'nnATC
Cuts at: 6 2661 4700 6
Size: 2655 2039 455
Fragments arranged by size:
2655 2039 455
BsaHI Gr'CG_yC
Cuts at: 2578 2826 3530 5026 2578
Size: 248 704 1496 2701
Fragments arranged by size:
2701 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1788 2336 2857 3537 3811 3958 4369 1788
Size: 548 521 680 274 147 411 2568
Fragments arranged by size:
2568 680 548 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1858 1975 1858
Size: 117 5032
BseYI C'CCAG_C
Cuts at: 1352 2572 3330 3909 4405 1352
Size: 1220 758 579 496 2096
Fragments arranged by size:
2096 1220 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1226 1226
Size: 5149
BsiEI CG_ry'CG
Cuts at: 104 2689 2735 3945 104
Size: 2585 46 1210 1308
Fragments arranged by size:
2585 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 451 1261 2161 2939 3129 3923 451
Size: 810 900 778 190 794 1677
Fragments arranged by size:
1677 900 810 794 778 190
BsmI GAATG_Cn'
Cuts at: 130 1941 130
Size: 1811 3338
Bsp1286I G_dGCh'C
Cuts at: 451 1261 1652 1862 2161 2772 2865 2939 3129
Size: 810 391 210 299 611 93 74 190
Cuts at: 3129 3191 3923 451
Size: 62 732 1677
Fragments arranged by size:
1677 810 732 611 391 299 210 190
93 74 62
BspEI T'CCGG_A
Cuts at: 4369 4369
Size: 5149
BspHI T'CATG_A
Cuts at: 1080 4325 1080
Size: 3245 1904
BspMI ACCTGCnnnn'nnnn_
Cuts at: 432 2118 2197 2411 2713 3094 432
Size: 1686 79 214 302 381 2487
Fragments arranged by size:
2487 1686 381 302 214 79
BsrBI CCG'CTC
Cuts at: 50 3439 3493 4979 5144 50
Size: 3389 54 1486 165 55
Fragments arranged by size:
3389 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3059 3059
Size: 5149
BsrFI r'CCGG_y
Cuts at: 1788 3145 3326 3537 1788
Size: 1357 181 211 3400
Fragments arranged by size:
3400 1357 211 181
BssSI C'ACGA_G
Cuts at: 3418 3778 3418
Size: 360 4789
BstAPI GCAn_nnn'nTGC
Cuts at: 2524 2524
Size: 5149
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5149
BstYI r'GATC_y
Cuts at: 1 524 2172 2639 2662 2997 3243 4257 4343
Size: 523 1648 467 23 335 246 1014 86
Cuts at: 4343 4355 4701 1
Size: 12 346 449
Fragments arranged by size:
1648 1014 523 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 791 1600 791
Size: 809 4340
Bsu36I CC'TnA_GG
Cuts at: 1568 1568
Size: 5149
BtgI C'CryG_G
Cuts at: 125 125
Size: 5149
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3097 3278 3441 3097
Size: 181 163 4805
Fragments arranged by size:
4805 181 163
BtsI GCAGTG_nn'
Cuts at: 1326 2390 1326
Size: 1064 4085
DraI TTT'AAA
Cuts at: 2146 2146
Size: 5149
DrdI GACnn_nn'nnGTC
Cuts at: 2853 3713 2853
Size: 860 4289
EaeI y'GGCC_r
Cuts at: 1129 1336 2732 2906 3297 3324 4671 1129
Size: 207 1396 174 391 27 1347 1607
Fragments arranged by size:
1607 1396 1347 391 207 174 27
EagI C'GGCC_G
Cuts at: 2732 2732
Size: 5149
EarI CTCTTCn'nnn_
Cuts at: 248 1448 1652 1886 3170 3380 248
Size: 1200 204 234 1284 210 2017
Fragments arranged by size:
2017 1284 1200 234 210 204
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2088 3355 3667 3813 4959 2088
Size: 1267 312 146 1146 2278
Fragments arranged by size:
2278 1267 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1259 2159 1259
Size: 900 4249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 362 742 980 1025 1991 2972 3404 4153
Size: 156 380 238 45 966 981 432 749
Cuts at: 4153 4421 206
Size: 268 934
Fragments arranged by size:
981 966 934 749 432 380 268 238
156 45
EcoO109I rG'GnC_Cy
Cuts at: 216 2107 2286 216
Size: 1891 179 3079
Fragments arranged by size:
3079 1891 179
EcoRI G'AATT_C
Cuts at: 1267 2151 1267
Size: 884 4265
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 772 825 1711 3166 772
Size: 53 886 1455 2755
Fragments arranged by size:
2755 1455 886 53
FspI TGC'GCA
Cuts at: 2928 2928
Size: 5149
HaeII r_GCGC'y
Cuts at: 2581 2829 3853 4832 2581
Size: 248 1024 979 2898
Fragments arranged by size:
2898 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 181 213 1000 1032 1075 1107 1651 1683 1789
Size: 32 787 32 43 32 544 32 106
Cuts at: 1789 1821 1837 1869 1951 1983 2017 2049 2989
Size: 32 16 32 82 32 34 32 940
Cuts at: 2989 3019 3021 3051 181
Size: 30 2 30 2279
Fragments arranged by size:
2279 940 787 544 106 82 43 34
32 32 32 32 32 32 32 32
30 30 16 2
HincII GTy'rAC
Cuts at: 2186 2186
Size: 5149
KasI G'GCGC_C
Cuts at: 2577 2825 2577
Size: 248 4901
KpnI G_GTAC'C
Cuts at: 873 2167 873
Size: 1294 3855
MfeI C'AATT_G
Cuts at: 652 652
Size: 5149
MluI A'CGCG_T
Cuts at: 4582 4582
Size: 5149
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 840 3820 4004 840
Size: 2980 184 1985
Fragments arranged by size:
2980 1985 184
MscI TGG'CCA
Cuts at: 2908 2908
Size: 5149
MslI CAynn'nnrTG
Cuts at: 534 3136 3263 534
Size: 2602 127 2420
Fragments arranged by size:
2602 2420 127
MspA1I CmG'CkG
Cuts at: 358 2258 2572 2932 3947 4192 358
Size: 1900 314 360 1015 245 1315
Fragments arranged by size:
1900 1315 1015 360 314 245
NaeI GCC'GGC
Cuts at: 3328 3328
Size: 5149
NarI GG'CG_CC
Cuts at: 2578 2826 2578
Size: 248 4901
NcoI C'CATG_G
Cuts at: 125 125
Size: 5149
NdeI CA'TA_TG
Cuts at: 622 622
Size: 5149
NgoMIV G'CCGG_C
Cuts at: 3326 3326
Size: 5149
NruI TCG'CGA
Cuts at: 3510 3510
Size: 5149
NspI r_CATG'y
Cuts at: 198 720 2200 2700 3609 198
Size: 522 1480 500 909 1738
Fragments arranged by size:
1738 1480 909 522 500
PciI A'CATG_T
Cuts at: 194 3605 194
Size: 3411 1738
PflMI CCAn_nnn'nTGG
Cuts at: 1138 1138
Size: 5149
PfoI T'CCnGG_A
Cuts at: 822 1271 822
Size: 449 4700
PmeI GTTT'AAAC
Cuts at: 2146 2146
Size: 5149
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4288 4320 4288
Size: 32 5117
PpuMI rG'GwC_Cy
Cuts at: 216 2107 216
Size: 1891 3258
PshAI GACnn'nnGTC
Cuts at: 954 954
Size: 5149
PsiI TTA'TAA
Cuts at: 1447 1447
Size: 5149
PstI C_TGCA'G
Cuts at: 2194 2879 2194
Size: 685 4464
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5149
PvuII CAG'CTG
Cuts at: 358 2572 2932 358
Size: 2214 360 2575
Fragments arranged by size:
2575 2214 360
RsrII CG'GwC_CG
Cuts at: 3342 3342
Size: 5149
SacI G_AGCT'C
Cuts at: 1261 2161 1261
Size: 900 4249
SalI G'TCGA_C
Cuts at: 2184 2184
Size: 5149
SanDI GG'GwC_CC
Cuts at: 216 216
Size: 5149
SapI GCTCTTCn'nnn_
Cuts at: 1886 3170 3380 1886
Size: 1284 210 3655
Fragments arranged by size:
3655 1284 210
SbfI CC_TGCA'GG
Cuts at: 2194 2194
Size: 5149
ScaI AGT'ACT
Cuts at: 549 4666 549
Size: 4117 1032
SfcI C'TryA_G
Cuts at: 33 611 2190 2875 3870 4061 33
Size: 578 1579 685 995 191 1121
Fragments arranged by size:
1579 1121 995 685 578 191
SfoI GGC'GCC
Cuts at: 2579 2827 2579
Size: 248 4901
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5149
SmaI CCC'GGG
Cuts at: 2169 2169
Size: 5149
SmlI C'TyrA_G
Cuts at: 436 1175 1432 2302 3217 3711 3973 436
Size: 739 257 870 915 494 262 1612
Fragments arranged by size:
1612 915 870 739 494 262 257
SnaBI TAC'GTA
Cuts at: 2139 2139
Size: 5149
SpeI A'CTAG_T
Cuts at: 67 1468 67
Size: 1401 3748
SphI G_CATG'C
Cuts at: 2200 2700 2200
Size: 500 4649
SspI AAT'ATT
Cuts at: 252 252
Size: 5149
StyI C'CwwG_G
Cuts at: 125 2281 2635 125
Size: 2156 354 2639
Fragments arranged by size:
2639 2156 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2730 2863 3322 3533 2730
Size: 133 459 211 4346
Fragments arranged by size:
4346 459 211 133
TatI w'GTAC_w
Cuts at: 547 4664 547
Size: 4117 1032
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4666 4698 4666
Size: 32 5117
Tth111I GACn'n_nGTC
Cuts at: 2944 2944
Size: 5149
XbaI T'CTAG_A
Cuts at: 2178 2178
Size: 5149
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2642 2642
Size: 5149
XmaI C'CCGG_G
Cuts at: 2167 2167
Size: 5149
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NruI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AleI AlfI AloI ApaI AscI AvrII
BbvCI BcgI BfrBI BglI BmtI Bpu10I BsaXI BsiWI
BsmBI BsrGI BssHII BstEII BstXI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI NheI NotI
NsiI PacI PasI PmlI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SrfI StuI SwaI XhoI XmnI
ZraI