Restriction map for
pF1KSDA0886
(Circular) (Six-base) MAPSORT of: pF1KSDA0886.seq Check: 5546 from: 1 to: 6713
pF1KSDA0886 6713 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 469 3751 469
Size: 3282 3431
Acc65I G'GTAC_C
Cuts at: 3729 3729
Size: 6713
AclI AA'CG_TT
Cuts at: 2698 6553 2698
Size: 3855 2858
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 929 1193 1571 1787 2036 3119 3383 3502 3555
Size: 264 378 216 249 1083 264 119 53
Cuts at: 3555 4538 4970 5717 5985 929
Size: 983 432 747 268 1657
Fragments arranged by size:
1657 1083 983 747 432 378 268 264
264 249 216 119 53
AflIII A'CryG_T
Cuts at: 1513 4065 5169 6146 1513
Size: 2552 1104 977 2080
Fragments arranged by size:
2552 2080 1104 977
AgeI A'CCGG_T
Cuts at: 5101 5101
Size: 6713
AleI CACnn'nnGTG
Cuts at: 2816 2816
Size: 6713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1929 2884 1929
Size: 955 5758
AlwNI CAG_nnn'CTG
Cuts at: 1060 1540 2311 4018 5585 1060
Size: 480 771 1707 1567 2188
Fragments arranged by size:
2188 1707 1567 771 480
ApaLI G'TGCA_C
Cuts at: 5483 5483
Size: 6713
ApoI r'AATT_y
Cuts at: 1986 2606 3717 6066 6322 1986
Size: 620 1111 2349 256 2377
Fragments arranged by size:
2377 2349 1111 620 256
AscI GG'CGCG_CC
Cuts at: 350 350
Size: 6713
AseI AT'TA_AT
Cuts at: 20 2174 20
Size: 2154 4559
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6713
AvaI C'yCGr_G
Cuts at: 217 395 453 669 3733 217
Size: 178 58 216 3064 3197
Fragments arranged by size:
3197 3064 216 178 58
AvrII C'CTAG_G
Cuts at: 1081 1081
Size: 6713
BamHI G'GATC_C
Cuts at: 3738 3738
Size: 6713
BanI G'GyrC_C
Cuts at: 390 3190 3446 3520 3729 4143 4391 4426 390
Size: 2800 256 74 209 414 248 35 2677
Fragments arranged by size:
2800 2677 414 256 248 209 74 35
BanII G_rGCy'C
Cuts at: 218 460 583 596 667 676 2630 3727 4757
Size: 242 123 13 71 9 1954 1097 1030
Cuts at: 4757 218
Size: 2174
Fragments arranged by size:
2174 1954 1097 1030 242 123 71 13
9
BbeI G_GCGC'C
Cuts at: 394 3450 4147 4395 394
Size: 3056 697 248 2712
Fragments arranged by size:
3056 2712 697 248
BbsI GAAGACnn'nnnn_
Cuts at: 137 272 1199 3178 137
Size: 135 927 1979 3672
Fragments arranged by size:
3672 1979 927 135
BciVI GTATCCnnnnn_n'
Cuts at: 2817 4611 5372 6713 2817
Size: 1794 761 1341 2817
Fragments arranged by size:
2817 1794 1341 761
BclI T'GATC_A
Cuts at: 2313 2747 4233 2313
Size: 434 1486 4793
Fragments arranged by size:
4793 1486 434
BglII A'GATC_T
Cuts at: 4228 6265 4228
Size: 2037 4676
BlpI GC'TnA_GC
Cuts at: 3825 3825
Size: 6713
Bme1580I G_kGCm'C
Cuts at: 332 479 4338 4431 5487 332
Size: 147 3859 93 1056 1558
Fragments arranged by size:
3859 1558 1056 147 93
BmrI ACTGGGnnnn_n'
Cuts at: 319 1463 3925 4006 4341 6485 319
Size: 1144 2462 81 335 2144 547
Fragments arranged by size:
2462 2144 1144 547 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5080 5080
Size: 6713
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 301 310 1370 1763 2966 3177 3197 292
Size: 9 9 1060 393 1203 211 20 3808
Fragments arranged by size:
3808 1203 1060 393 211 20 9 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1432 2990 3889 4768 5260 5558 1432
Size: 1558 899 879 492 298 2587
Fragments arranged by size:
2587 1558 899 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 159 679 957 6417 159
Size: 520 278 5460 455
Fragments arranged by size:
5460 520 455 278
BsaAI yAC'GTr
Cuts at: 3705 4066 3705
Size: 361 6352
BsaBI GATnn'nnATC
Cuts at: 6 3099 3617 4227 6264 6
Size: 3093 518 610 2037 455
Fragments arranged by size:
3093 2037 610 518 455
BsaHI Gr'CG_yC
Cuts at: 391 3447 4144 4392 5094 6590 391
Size: 3056 697 248 702 1496 514
Fragments arranged by size:
3056 1496 702 697 514 248
BsaWI w'CCGG_w
Cuts at: 2262 3902 4423 5101 5375 5522 5933 2262
Size: 1640 521 678 274 147 411 3042
Fragments arranged by size:
3042 1640 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1503 1533 2184 2214 1503
Size: 30 651 30 6002
Fragments arranged by size:
6002 651 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 137 241 253 256 265 289 551 657 720
Size: 104 12 3 9 24 262 106 63
Cuts at: 720 833 2638 3394 137
Size: 113 1805 756 3456
Fragments arranged by size:
3456 1805 756 262 113 106 104 63
24 12 9 3
BseYI C'CCAG_C
Cuts at: 1530 2026 2845 4138 4896 5473 5969 1530
Size: 496 819 1293 758 577 496 2274
Fragments arranged by size:
2274 1293 819 758 577 496 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3411 3411
Size: 6713
BsiEI CG_ry'CG
Cuts at: 104 181 413 473 647 4255 4301 5509 104
Size: 77 232 60 174 3608 46 1208 1308
Fragments arranged by size:
3608 1308 1208 232 174 77 60 46
BsiHKAI G_wGCw'C
Cuts at: 1521 2630 2972 3727 4505 4695 5487 1521
Size: 1109 342 755 778 190 792 2747
Fragments arranged by size:
2747 1109 792 778 755 342 190
Bsp1286I G_dGCh'C
Cuts at: 218 332 460 479 583 596 667 676 1521
Size: 114 128 19 104 13 71 9 845
Cuts at: 1521 2630 2972 3727 4338 4431 4505 4695 4757
Size: 1109 342 755 611 93 74 190 62
Cuts at: 4757 5487 218
Size: 730 1444
Fragments arranged by size:
1444 1109 845 755 730 611 342 190
128 114 104 93 74 71 62 19
13 9
BspEI T'CCGG_A
Cuts at: 2262 5933 2262
Size: 3671 3042
BspHI T'CATG_A
Cuts at: 5889 5889
Size: 6713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3763 3977 4279 4660 3763
Size: 214 302 381 5816
Fragments arranged by size:
5816 381 302 214
BsrBI CCG'CTC
Cuts at: 50 415 435 5005 5059 6543 6708 50
Size: 365 20 4570 54 1484 165 55
Fragments arranged by size:
4570 1484 365 165 55 54 20
BsrDI GCAATG_nn'
Cuts at: 1547 2851 3251 4625 1547
Size: 1304 400 1374 3635
Fragments arranged by size:
3635 1374 1304 400
BsrFI r'CCGG_y
Cuts at: 346 387 408 4711 4892 5101 346
Size: 41 21 4303 181 209 1958
Fragments arranged by size:
4303 1958 209 181 41 21
BssHII G'CGCG_C
Cuts at: 350 350
Size: 6713
BssSI C'ACGA_G
Cuts at: 4984 5342 4984
Size: 358 6355
BstAPI GCAn_nnn'nTGC
Cuts at: 1060 4090 1060
Size: 3030 3683
BstBI TT'CG_AA
Cuts at: 110 5074 110
Size: 4964 1749
BstYI r'GATC_y
Cuts at: 1 1578 3738 4205 4228 4563 4809 5821 5907
Size: 1577 2160 467 23 335 246 1012 86
Cuts at: 5907 5919 6265 1
Size: 12 346 449
Fragments arranged by size:
2160 1577 1012 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 527 2504 527
Size: 1977 4736
BtgI C'CryG_G
Cuts at: 125 317 125
Size: 192 6521
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4663 4844 5007 4663
Size: 181 163 6369
Fragments arranged by size:
6369 181 163
BtsI GCAGTG_nn'
Cuts at: 1256 3497 3956 1256
Size: 2241 459 4013
Fragments arranged by size:
4013 2241 459
DraI TTT'AAA
Cuts at: 3712 3712
Size: 6713
DrdI GACnn_nn'nnGTC
Cuts at: 4419 5277 4419
Size: 858 5855
EaeI y'GGCC_r
Cuts at: 178 410 569 644 2999 4298 4472 4863 4890
Size: 232 159 75 2355 1299 174 391 27
Cuts at: 4890 6235 178
Size: 1345 656
Fragments arranged by size:
2355 1345 1299 656 391 232 174 159
75 27
EagI C'GGCC_G
Cuts at: 178 410 644 4298 178
Size: 232 234 3654 2593
Fragments arranged by size:
3654 2593 234 232
EarI CTCTTCn'nnn_
Cuts at: 230 242 704 833 1112 1922 2099 2189 2906
Size: 12 462 129 279 810 177 90 717
Cuts at: 2906 3457 3574 4736 4946 230
Size: 551 117 1162 210 1997
Fragments arranged by size:
1997 1162 810 717 551 462 279 210
177 129 117 90 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4921 5231 5377 6523 4921
Size: 310 146 1146 5111
Fragments arranged by size:
5111 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2628 3725 2628
Size: 1097 5616
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 301 310 929 1193 1370 1571 1763 1787
Size: 9 9 619 264 177 201 192 24
Cuts at: 1787 2036 2966 3119 3177 3197 3383 3502 3555
Size: 249 930 153 58 20 186 119 53
Cuts at: 3555 4538 4970 5717 5985 292
Size: 983 432 747 268 1020
Fragments arranged by size:
1020 983 930 747 619 432 268 264
249 201 192 186 177 153 119 58
53 24 20 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1571 2507 1571
Size: 936 5777
EcoO109I rG'GnC_Cy
Cuts at: 400 450 3852 400
Size: 50 3402 3261
Fragments arranged by size:
3402 3261 50
EcoRI G'AATT_C
Cuts at: 1986 3717 1986
Size: 1731 4982
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 934 2988 4732 934
Size: 2054 1744 2915
Fragments arranged by size:
2915 2054 1744
FspI TGC'GCA
Cuts at: 4494 4494
Size: 6713
HaeII r_GCGC'y
Cuts at: 394 3450 4147 4395 5417 6396 394
Size: 3056 697 248 1022 979 711
Fragments arranged by size:
3056 1022 979 711 697 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 784 816 1343 1375 1493 1525 3079 3111 3540
Size: 32 527 32 118 32 1554 32 429
Cuts at: 3540 3572 4555 4585 4587 4617 784
Size: 32 983 30 2 30 2880
Fragments arranged by size:
2880 1554 983 527 429 118 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 470 893 2361 3144 3752 470
Size: 423 1468 783 608 3431
Fragments arranged by size:
3431 1468 783 608 423
HindIII A'AGCT_T
Cuts at: 938 2045 2242 938
Size: 1107 197 5409
Fragments arranged by size:
5409 1107 197
KasI G'GCGC_C
Cuts at: 390 3446 4143 4391 390
Size: 3056 697 248 2712
Fragments arranged by size:
3056 2712 697 248
KpnI G_GTAC'C
Cuts at: 3733 3733
Size: 6713
MfeI C'AATT_G
Cuts at: 2717 2717
Size: 6713
MluI A'CGCG_T
Cuts at: 6146 6146
Size: 6713
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5384 5568 5384
Size: 184 6529
MscI TGG'CCA
Cuts at: 3001 4474 3001
Size: 1473 5240
MslI CAynn'nnrTG
Cuts at: 1969 2816 4702 4829 1969
Size: 847 1886 127 3853
Fragments arranged by size:
3853 1886 847 127
MspA1I CmG'CkG
Cuts at: 319 492 3824 4138 4498 5511 5756 319
Size: 173 3332 314 360 1013 245 1276
Fragments arranged by size:
3332 1276 1013 360 314 245 173
NaeI GCC'GGC
Cuts at: 348 389 410 4894 348
Size: 41 21 4484 2167
Fragments arranged by size:
4484 2167 41 21
NarI GG'CG_CC
Cuts at: 391 3447 4144 4392 391
Size: 3056 697 248 2712
Fragments arranged by size:
3056 2712 697 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 6713
NdeI CA'TA_TG
Cuts at: 2100 2100
Size: 6713
NgoMIV G'CCGG_C
Cuts at: 346 387 408 4892 346
Size: 41 21 4484 2167
Fragments arranged by size:
4484 2167 41 21
NspI r_CATG'y
Cuts at: 1517 1764 1802 2222 3766 4266 5173 1517
Size: 247 38 420 1544 500 907 3057
Fragments arranged by size:
3057 1544 907 500 420 247 38
PacI TTA_AT'TAA
Cuts at: 2231 2231
Size: 6713
PciI A'CATG_T
Cuts at: 1513 5169 1513
Size: 3656 3057
PmeI GTTT'AAAC
Cuts at: 3712 3712
Size: 6713
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5852 5884 5852
Size: 32 6681
PpuMI rG'GwC_Cy
Cuts at: 400 450 400
Size: 50 6663
PstI C_TGCA'G
Cuts at: 738 1905 1995 2640 3760 4445 738
Size: 1167 90 645 1120 685 3006
Fragments arranged by size:
3006 1167 1120 685 645 90
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6713
PvuII CAG'CTG
Cuts at: 4138 4498 4138
Size: 360 6353
RsrII CG'GwC_CG
Cuts at: 4908 4908
Size: 6713
SacI G_AGCT'C
Cuts at: 2630 3727 2630
Size: 1097 5616
SacII CC_GC'GG
Cuts at: 320 320
Size: 6713
SalI G'TCGA_C
Cuts at: 468 3750 468
Size: 3282 3431
SanDI GG'GwC_CC
Cuts at: 400 450 400
Size: 50 6663
SapI GCTCTTCn'nnn_
Cuts at: 704 2189 4736 4946 704
Size: 1485 2547 210 2471
Fragments arranged by size:
2547 2471 1485 210
SbfI CC_TGCA'GG
Cuts at: 3760 3760
Size: 6713
ScaI AGT'ACT
Cuts at: 2634 6230 2634
Size: 3596 3117
SfcI C'TryA_G
Cuts at: 33 734 1166 1901 1991 2636 3756 4441 5434
Size: 701 432 735 90 645 1120 685 993
Cuts at: 5434 5625 33
Size: 191 1121
Fragments arranged by size:
1121 1120 993 735 701 685 645 432
191 90
SfoI GGC'GCC
Cuts at: 392 3448 4145 4393 392
Size: 3056 697 248 2712
Fragments arranged by size:
3056 2712 697 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6713
SgrAI Cr'CCGG_yG
Cuts at: 346 387 346
Size: 41 6672
SmaI CCC'GGG
Cuts at: 397 3735 397
Size: 3338 3375
SmlI C'TyrA_G
Cuts at: 1411 3005 3868 4783 5275 5537 1411
Size: 1594 863 915 492 262 2587
Fragments arranged by size:
2587 1594 915 863 492 262
SnaBI TAC'GTA
Cuts at: 3705 3705
Size: 6713
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6713
SphI G_CATG'C
Cuts at: 3766 4266 3766
Size: 500 6213
SspI AAT'ATT
Cuts at: 2172 2172
Size: 6713
StuI AGG'CCT
Cuts at: 1772 1772
Size: 6713
StyI C'CwwG_G
Cuts at: 125 879 1081 3257 3847 4201 125
Size: 754 202 2176 590 354 2637
Fragments arranged by size:
2637 2176 754 590 354 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1615 3353 4296 4429 4888 5097 1615
Size: 1738 943 133 459 209 3231
Fragments arranged by size:
3231 1738 943 459 209 133
TatI w'GTAC_w
Cuts at: 1788 2632 3152 3395 6228 1788
Size: 844 520 243 2833 2273
Fragments arranged by size:
2833 2273 844 520 243
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1503 1535 6230 6262 1503
Size: 32 4695 32 1954
Fragments arranged by size:
4695 1954 32 32
Tth111I GACn'n_nGTC
Cuts at: 4510 4510
Size: 6713
XbaI T'CTAG_A
Cuts at: 3744 3744
Size: 6713
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 4208 132
Size: 4076 2637
XmaI C'CCGG_G
Cuts at: 395 3733 395
Size: 3338 3375
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PacI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI BaeI
BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsiWI
BsmI BsmBI BsrGI BstEII BstXI BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HpaI NheI NotI NruI
NsiI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SexAI SfiI SrfI SwaI XhoI XmnI
ZraI