Restriction map for
pF1KSDA0885
(Circular) (Six-base) MAPSORT of: pF1KSDA0885.seq Check: 3545 from: 1 to: 5533
pF1KSDA0885 5533 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 2569 2569
Size: 5533
Acc65I G'GTAC_C
Cuts at: 170 2547 170
Size: 2377 3156
AclI AA'CG_TT
Cuts at: 572 5373 572
Size: 4801 732
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 434 575 1236 1723 3356 3788 4537 4805
Size: 264 141 661 487 1633 432 749 268
Cuts at: 4805 170
Size: 898
Fragments arranged by size:
1633 898 749 661 487 432 268 264
141
AflIII A'CryG_T
Cuts at: 2883 3989 4966 2883
Size: 1106 977 3450
Fragments arranged by size:
3450 1106 977
AgeI A'CCGG_T
Cuts at: 3921 3921
Size: 5533
AleI CACnn'nnGTG
Cuts at: 764 1253 764
Size: 489 5044
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 670 670
Size: 5533
AlwNI CAG_nnn'CTG
Cuts at: 2321 2836 4405 2321
Size: 515 1569 3449
Fragments arranged by size:
3449 1569 515
ApaI G_GGCC'C
Cuts at: 2350 2350
Size: 5533
ApaLI G'TGCA_C
Cuts at: 4303 4303
Size: 5533
ApoI r'AATT_y
Cuts at: 334 769 814 1259 1594 2535 4886 5142 334
Size: 435 45 445 335 941 2351 256 725
Fragments arranged by size:
2351 941 725 445 435 335 256 45
AseI AT'TA_AT
Cuts at: 20 20
Size: 5533
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5533
AvaI C'yCGr_G
Cuts at: 483 1166 2551 483
Size: 683 1385 3465
Fragments arranged by size:
3465 1385 683
BamHI G'GATC_C
Cuts at: 2556 2556
Size: 5533
BanI G'GyrC_C
Cuts at: 170 2547 2961 3209 3244 170
Size: 2377 414 248 35 2459
Fragments arranged by size:
2459 2377 414 248 35
BanII G_rGCy'C
Cuts at: 2350 2545 3575 2350
Size: 195 1030 4308
Fragments arranged by size:
4308 1030 195
BbeI G_GCGC'C
Cuts at: 2965 3213 2965
Size: 248 5285
BbvCI CC'TCA_GC
Cuts at: 1487 1487
Size: 5533
BciVI GTATCCnnnnn_n'
Cuts at: 3429 4192 5533 3429
Size: 763 1341 3429
Fragments arranged by size:
3429 1341 763
BclI T'GATC_A
Cuts at: 3051 3051
Size: 5533
BglII A'GATC_T
Cuts at: 3046 5085 3046
Size: 2039 3494
BlpI GC'TnA_GC
Cuts at: 2643 2643
Size: 5533
Bme1580I G_kGCm'C
Cuts at: 1375 1417 2350 3156 3249 4307 1375
Size: 42 933 806 93 1058 2601
Fragments arranged by size:
2601 1058 933 806 93 42
BmgBI CAC'GTC
Cuts at: 1099 1099
Size: 5533
BmrI ACTGGGnnnn_n'
Cuts at: 215 371 944 1807 2743 2824 3159 5305 215
Size: 156 573 863 936 81 335 2146 443
Fragments arranged by size:
2146 936 863 573 443 335 156 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 488 3898 488
Size: 3410 2123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 608 458
Size: 150 5383
Bpu10I CC'TnA_GC
Cuts at: 1487 1487
Size: 5533
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 857 2707 3586 4080 4378 857
Size: 1850 879 494 298 2012
Fragments arranged by size:
2012 1850 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 5237 5237
Size: 5533
BsaAI yAC'GTr
Cuts at: 2523 2884 2523
Size: 361 5172
BsaBI GATnn'nnATC
Cuts at: 6 3045 5084 6
Size: 3039 2039 455
Fragments arranged by size:
3039 2039 455
BsaHI Gr'CG_yC
Cuts at: 2962 3210 3914 5410 2962
Size: 248 704 1496 3085
Fragments arranged by size:
3085 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2720 3241 3921 4195 4342 4753 2720
Size: 521 680 274 147 411 3500
Fragments arranged by size:
3500 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 314 344 1574 1604 2258 2288 314
Size: 30 1230 30 654 30 3559
Fragments arranged by size:
3559 1230 654 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1562 1956 2008 2357 2417 1562
Size: 394 52 349 60 4678
Fragments arranged by size:
4678 394 349 60 52
BseYI C'CCAG_C
Cuts at: 2956 3714 4293 4789 2956
Size: 758 579 496 3700
Fragments arranged by size:
3700 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1871 2334 2504 1871
Size: 463 170 4900
Fragments arranged by size:
4900 463 170
BsiEI CG_ry'CG
Cuts at: 104 3073 3119 4329 104
Size: 2969 46 1210 1308
Fragments arranged by size:
2969 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 645 2427 2545 3323 3513 4307 645
Size: 1782 118 778 190 794 1871
Fragments arranged by size:
1871 1782 794 778 190 118
Bsp1286I G_dGCh'C
Cuts at: 645 1375 1417 2350 2427 2545 3156 3249 3323
Size: 730 42 933 77 118 611 93 74
Cuts at: 3323 3513 3575 4307 645
Size: 190 62 732 1871
Fragments arranged by size:
1871 933 732 730 611 190 118 93
77 74 62 42
BspEI T'CCGG_A
Cuts at: 4753 4753
Size: 5533
BspHI T'CATG_A
Cuts at: 1737 4709 1737
Size: 2972 2561
BspMI ACCTGCnnnn'nnnn_
Cuts at: 311 1620 2581 2795 3097 3478 311
Size: 1309 961 214 302 381 2366
Fragments arranged by size:
2366 1309 961 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3823 3877 5363 5528 50
Size: 3773 54 1486 165 55
Fragments arranged by size:
3773 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 371 970 3443 371
Size: 599 2473 2461
Fragments arranged by size:
2473 2461 599
BsrFI r'CCGG_y
Cuts at: 3529 3710 3921 3529
Size: 181 211 5141
Fragments arranged by size:
5141 211 181
BssSI C'ACGA_G
Cuts at: 3802 4162 3802
Size: 360 5173
BstAPI GCAn_nnn'nTGC
Cuts at: 2908 2908
Size: 5533
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5533
BstEII G'GTnAC_C
Cuts at: 1047 1063 1047
Size: 16 5517
BstXI CCAn_nnnn'nTGG
Cuts at: 157 1554 157
Size: 1397 4136
BstYI r'GATC_y
Cuts at: 1 1561 2495 2556 3023 3046 3381 3627 4641
Size: 1560 934 61 467 23 335 246 1014
Cuts at: 4641 4727 4739 5085 1
Size: 86 12 346 449
Fragments arranged by size:
1560 1014 934 467 449 346 335 246
86 61 23 12
Bsu36I CC'TnA_GG
Cuts at: 1939 1939
Size: 5533
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 818 3481 3662 3825 818
Size: 2663 181 163 2526
Fragments arranged by size:
2663 2526 181 163
BtsI GCAGTG_nn'
Cuts at: 193 2774 193
Size: 2581 2952
DraI TTT'AAA
Cuts at: 2530 2530
Size: 5533
DraIII CAC_nnn'GTG
Cuts at: 199 1878 199
Size: 1679 3854
DrdI GACnn_nn'nnGTC
Cuts at: 1655 3237 4097 1655
Size: 1582 860 3091
Fragments arranged by size:
3091 1582 860
EaeI y'GGCC_r
Cuts at: 2042 3116 3290 3681 3708 5055 2042
Size: 1074 174 391 27 1347 2520
Fragments arranged by size:
2520 1347 1074 391 174 27
EagI C'GGCC_G
Cuts at: 3116 3116
Size: 5533
EarI CTCTTCn'nnn_
Cuts at: 3554 3764 3554
Size: 210 5323
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3739 4051 4197 5343 3739
Size: 312 146 1146 3929
Fragments arranged by size:
3929 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2543 2543
Size: 5533
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 434 458 575 608 1236 1723 3356 3788
Size: 264 24 117 33 628 487 1633 432
Cuts at: 3788 4537 4805 170
Size: 749 268 898
Fragments arranged by size:
1633 898 749 628 487 432 268 264
117 33 24
EcoNI CCTnn'n_nnAGG
Cuts at: 1577 1577
Size: 5533
EcoO109I rG'GnC_Cy
Cuts at: 2346 2347 2670 2346
Size: 1 323 5209
Fragments arranged by size:
5209 323 1
EcoRI G'AATT_C
Cuts at: 814 2535 814
Size: 1721 3812
EcoRV GAT'ATC
Cuts at: 897 897
Size: 5533
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 286 798 1698 1767 2235 3550 286
Size: 512 900 69 468 1315 2269
Fragments arranged by size:
2269 1315 900 512 468 69
FspI TGC'GCA
Cuts at: 2146 3312 2146
Size: 1166 4367
HaeII r_GCGC'y
Cuts at: 2321 2965 3213 4237 5216 2321
Size: 644 248 1024 979 2638
Fragments arranged by size:
2638 1024 979 644 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 120 152 454 486 1024 1056 1309 1341 1935
Size: 32 302 32 538 32 253 32 594
Cuts at: 1935 1967 2280 2312 3373 3403 3405 3435 120
Size: 32 313 32 1061 30 2 30 2218
Fragments arranged by size:
2218 1061 594 538 313 302 253 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 227 993 2570 227
Size: 766 1577 3190
Fragments arranged by size:
3190 1577 766
HpaI GTT'AAC
Cuts at: 227 227
Size: 5533
KasI G'GCGC_C
Cuts at: 2961 3209 2961
Size: 248 5285
KpnI G_GTAC'C
Cuts at: 174 2551 174
Size: 2377 3156
MfeI C'AATT_G
Cuts at: 2089 2089
Size: 5533
MluI A'CGCG_T
Cuts at: 4966 4966
Size: 5533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 520 1978 4204 4388 520
Size: 1458 2226 184 1665
Fragments arranged by size:
2226 1665 1458 184
MscI TGG'CCA
Cuts at: 2044 3292 2044
Size: 1248 4285
MslI CAynn'nnrTG
Cuts at: 155 629 764 1253 1763 3520 3647 155
Size: 474 135 489 510 1757 127 2041
Fragments arranged by size:
2041 1757 510 489 474 135 127
MspA1I CmG'CkG
Cuts at: 478 579 680 2642 2956 3316 4331 4576 478
Size: 101 101 1962 314 360 1015 245 1435
Fragments arranged by size:
1962 1435 1015 360 314 245 101 101
NaeI GCC'GGC
Cuts at: 3712 3712
Size: 5533
NarI GG'CG_CC
Cuts at: 2962 3210 2962
Size: 248 5285
NgoMIV G'CCGG_C
Cuts at: 3710 3710
Size: 5533
NruI TCG'CGA
Cuts at: 3894 3894
Size: 5533
NspI r_CATG'y
Cuts at: 2584 3084 3993 2584
Size: 500 909 4124
Fragments arranged by size:
4124 909 500
PciI A'CATG_T
Cuts at: 3989 3989
Size: 5533
PmeI GTTT'AAAC
Cuts at: 2530 2530
Size: 5533
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2263 2295 4672 4704 2263
Size: 32 2377 32 3092
Fragments arranged by size:
3092 2377 32 32
PspOMI G'GGCC_C
Cuts at: 2346 2346
Size: 5533
PstI C_TGCA'G
Cuts at: 2066 2578 3263 2066
Size: 512 685 4336
Fragments arranged by size:
4336 685 512
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5533
PvuII CAG'CTG
Cuts at: 478 579 2956 3316 478
Size: 101 2377 360 2695
Fragments arranged by size:
2695 2377 360 101
RsrII CG'GwC_CG
Cuts at: 351 3726 351
Size: 3375 2158
SacI G_AGCT'C
Cuts at: 2545 2545
Size: 5533
SalI G'TCGA_C
Cuts at: 2568 2568
Size: 5533
SapI GCTCTTCn'nnn_
Cuts at: 3554 3764 3554
Size: 210 5323
SbfI CC_TGCA'GG
Cuts at: 2578 2578
Size: 5533
ScaI AGT'ACT
Cuts at: 5050 5050
Size: 5533
SfcI C'TryA_G
Cuts at: 33 762 2062 2257 2574 3259 4254 4445 33
Size: 729 1300 195 317 685 995 191 1121
Fragments arranged by size:
1300 1121 995 729 685 317 195 191
SfoI GGC'GCC
Cuts at: 2963 3211 2963
Size: 248 5285
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5533
SmaI CCC'GGG
Cuts at: 485 2553 485
Size: 2068 3465
SmlI C'TyrA_G
Cuts at: 872 2686 3601 4095 4357 872
Size: 1814 915 494 262 2048
Fragments arranged by size:
2048 1814 915 494 262
SnaBI TAC'GTA
Cuts at: 2523 2523
Size: 5533
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5533
SphI G_CATG'C
Cuts at: 2584 3084 2584
Size: 500 5033
SspI AAT'ATT
Cuts at: 1083 1083
Size: 5533
StuI AGG'CCT
Cuts at: 1620 1620
Size: 5533
StyI C'CwwG_G
Cuts at: 1043 1367 1547 1971 2351 2452 2665 3019 1043
Size: 324 180 424 380 101 213 354 3557
Fragments arranged by size:
3557 424 380 354 324 213 180 101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 368 1109 1165 3114 3247 3706 3917 368
Size: 741 56 1949 133 459 211 1984
Fragments arranged by size:
1984 1949 741 459 211 133 56
TatI w'GTAC_w
Cuts at: 5048 5048
Size: 5533
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 422 454 2258 2290 5050 5082 422
Size: 32 1804 32 2760 32 873
Fragments arranged by size:
2760 1804 873 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1807 3328 1807
Size: 1521 4012
XbaI T'CTAG_A
Cuts at: 2562 2562
Size: 5533
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1680 3026 1680
Size: 1346 4187
XmaI C'CCGG_G
Cuts at: 483 2551 483
Size: 2068 3465
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NruI NspI PciI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BglI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstZ17I BtgI ClaI CspCI FseI
FspAI HindIII NcoI NdeI NheI NotI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI SwaI
XhoI XmnI ZraI