Restriction map for
pF1KSDA0883
(Circular) (Six-base) MAPSORT of: pF1KSDA0883.seq Check: 8225 from: 1 to: 4231
pF1KSDA0883 4231 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 1267 1267
Size: 4231
Acc65I G'GTAC_C
Cuts at: 1245 1245
Size: 4231
AclI AA'CG_TT
Cuts at: 4071 4071
Size: 4231
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 910 1090 2054 2486 3235 3503 226
Size: 684 180 964 432 749 268 954
Fragments arranged by size:
964 954 749 684 432 268 180
AflIII A'CryG_T
Cuts at: 1581 2687 3664 1581
Size: 1106 977 2148
Fragments arranged by size:
2148 1106 977
AgeI A'CCGG_T
Cuts at: 2619 2619
Size: 4231
AhdI GACnn_n'nnGTC
Cuts at: 801 801
Size: 4231
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 323 323
Size: 4231
AlwNI CAG_nnn'CTG
Cuts at: 594 1047 1534 3103 594
Size: 453 487 1569 1722
Fragments arranged by size:
1722 1569 487 453
ApaI G_GGCC'C
Cuts at: 763 1084 763
Size: 321 3910
ApaLI G'TGCA_C
Cuts at: 3001 3001
Size: 4231
ApoI r'AATT_y
Cuts at: 1233 3584 3840 1233
Size: 2351 256 1624
Fragments arranged by size:
2351 1624 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4231
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4231
AvaI C'yCGr_G
Cuts at: 477 1249 477
Size: 772 3459
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 332 365 332
Size: 33 4198
BamHI G'GATC_C
Cuts at: 1254 1254
Size: 4231
BanI G'GyrC_C
Cuts at: 1052 1245 1659 1907 1942 1052
Size: 193 414 248 35 3341
Fragments arranged by size:
3341 414 248 193 35
BanII G_rGCy'C
Cuts at: 495 763 1084 1243 2273 495
Size: 268 321 159 1030 2453
Fragments arranged by size:
2453 1030 321 268 159
BbeI G_GCGC'C
Cuts at: 1663 1911 1663
Size: 248 3983
BbsI GAAGACnn'nnnn_
Cuts at: 898 898
Size: 4231
BbvCI CC'TCA_GC
Cuts at: 587 587
Size: 4231
BciVI GTATCCnnnnn_n'
Cuts at: 194 2127 2890 4231 194
Size: 1933 763 1341 194
Fragments arranged by size:
1933 1341 763 194
BclI T'GATC_A
Cuts at: 1749 1749
Size: 4231
BglI GCCn_nnn'nGGC
Cuts at: 758 758
Size: 4231
BglII A'GATC_T
Cuts at: 1744 3783 1744
Size: 2039 2192
BlpI GC'TnA_GC
Cuts at: 1341 1341
Size: 4231
Bme1580I G_kGCm'C
Cuts at: 530 763 1084 1854 1947 3005 530
Size: 233 321 770 93 1058 1756
Fragments arranged by size:
1756 1058 770 321 233 93
BmrI ACTGGGnnnn_n'
Cuts at: 356 1441 1522 1857 4003 356
Size: 1085 81 335 2146 584
Fragments arranged by size:
2146 1085 584 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 936 2596 936
Size: 1660 2571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 500 1030 500
Size: 530 3701
Bpu10I CC'TnA_GC
Cuts at: 587 1060 587
Size: 473 3758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1120 1405 2284 2778 3076 1120
Size: 285 879 494 298 2275
Fragments arranged by size:
2275 879 494 298 285
BsaI GGTCTCn'nnnn_
Cuts at: 480 924 3935 480
Size: 444 3011 776
Fragments arranged by size:
3011 776 444
BsaAI yAC'GTr
Cuts at: 1221 1582 1221
Size: 361 3870
BsaBI GATnn'nnATC
Cuts at: 6 1743 3782 6
Size: 1737 2039 455
Fragments arranged by size:
2039 1737 455
BsaHI Gr'CG_yC
Cuts at: 1660 1908 2612 4108 1660
Size: 248 704 1496 1783
Fragments arranged by size:
1783 1496 704 248
BsaWI w'CCGG_w
Cuts at: 293 611 953 1418 1939 2619 2893 3040 3451
Size: 318 342 465 521 680 274 147 411
Cuts at: 3451 293
Size: 1073
Fragments arranged by size:
1073 680 521 465 411 342 318 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 496 526 902 932 496
Size: 30 376 30 3795
Fragments arranged by size:
3795 376 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 232 1144 232
Size: 912 3319
BseYI C'CCAG_C
Cuts at: 645 1024 1090 1654 2412 2991 3487 645
Size: 379 66 564 758 579 496 1389
Fragments arranged by size:
1389 758 579 564 496 379 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 808 174
Size: 634 3597
BsiEI CG_ry'CG
Cuts at: 104 1771 1817 3027 104
Size: 1667 46 1210 1308
Fragments arranged by size:
1667 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1243 2021 2211 3005 1243
Size: 778 190 794 2469
Fragments arranged by size:
2469 794 778 190
BsmI GAATG_Cn'
Cuts at: 312 312
Size: 4231
BsmBI CGTCTCn'nnnn_
Cuts at: 1205 1205
Size: 4231
Bsp1286I G_dGCh'C
Cuts at: 495 530 763 1084 1243 1854 1947 2021 2211
Size: 35 233 321 159 611 93 74 190
Cuts at: 2211 2273 3005 495
Size: 62 732 1721
Fragments arranged by size:
1721 732 611 321 233 190 159 93
74 62 35
BspEI T'CCGG_A
Cuts at: 293 953 3451 293
Size: 660 2498 1073
Fragments arranged by size:
2498 1073 660
BspHI T'CATG_A
Cuts at: 1203 3407 1203
Size: 2204 2027
BspMI ACCTGCnnnn'nnnn_
Cuts at: 257 1005 1279 1493 1795 2176 257
Size: 748 274 214 302 381 2312
Fragments arranged by size:
2312 748 381 302 274 214
BsrBI CCG'CTC
Cuts at: 50 961 2521 2575 4061 4226 50
Size: 911 1560 54 1486 165 55
Fragments arranged by size:
1560 1486 911 165 55 54
BsrDI GCAATG_nn'
Cuts at: 578 2141 578
Size: 1563 2668
BsrFI r'CCGG_y
Cuts at: 204 1055 2227 2408 2619 204
Size: 851 1172 181 211 1816
Fragments arranged by size:
1816 1172 851 211 181
BssSI C'ACGA_G
Cuts at: 2500 2860 2500
Size: 360 3871
BstAPI GCAn_nnn'nTGC
Cuts at: 786 1606 786
Size: 820 3411
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4231
BstXI CCAn_nnnn'nTGG
Cuts at: 132 132
Size: 4231
BstYI r'GATC_y
Cuts at: 1 1254 1721 1744 2079 2325 3339 3425 3437
Size: 1253 467 23 335 246 1014 86 12
Cuts at: 3437 3783 1
Size: 346 449
Fragments arranged by size:
1253 1014 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 689 125
Size: 564 3667
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2179 2360 2523 2179
Size: 181 163 3887
Fragments arranged by size:
3887 181 163
BtsI GCAGTG_nn'
Cuts at: 1472 1472
Size: 4231
DraI TTT'AAA
Cuts at: 254 1228 254
Size: 974 3257
DraIII CAC_nnn'GTG
Cuts at: 786 786
Size: 4231
DrdI GACnn_nn'nnGTC
Cuts at: 255 1935 2795 255
Size: 1680 860 1691
Fragments arranged by size:
1691 1680 860
EaeI y'GGCC_r
Cuts at: 353 709 1191 1814 1988 2379 2406 3753 353
Size: 356 482 623 174 391 27 1347 831
Fragments arranged by size:
1347 831 623 482 391 356 174 27
EagI C'GGCC_G
Cuts at: 1814 1814
Size: 4231
EarI CTCTTCn'nnn_
Cuts at: 653 812 1121 1130 1160 2252 2462 653
Size: 159 309 9 30 1092 210 2422
Fragments arranged by size:
2422 1092 309 210 159 30 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 223 760 994 2437 2749 2895 4041 223
Size: 537 234 1443 312 146 1146 413
Fragments arranged by size:
1443 1146 537 413 312 234 146
EcoICRI GAG'CTC
Cuts at: 1241 1241
Size: 4231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 500 910 1030 1090 2054 2486 3235 3503
Size: 274 410 120 60 964 432 749 268
Cuts at: 3503 226
Size: 954
Fragments arranged by size:
964 954 749 432 410 274 268 120
60
EcoNI CCTnn'n_nnAGG
Cuts at: 499 1012 499
Size: 513 3718
EcoO109I rG'GnC_Cy
Cuts at: 237 759 760 1080 1368 237
Size: 522 1 320 288 3100
Fragments arranged by size:
3100 522 320 288 1
EcoRI G'AATT_C
Cuts at: 1233 1233
Size: 4231
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 417 2248 417
Size: 1831 2400
FspI TGC'GCA
Cuts at: 2010 2010
Size: 4231
HaeII r_GCGC'y
Cuts at: 133 1663 1911 2935 3914 133
Size: 1530 248 1024 979 450
Fragments arranged by size:
1530 1024 979 450 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 166 198 399 431 496 528 646 678 787
Size: 32 201 32 65 32 118 32 109
Cuts at: 787 819 902 934 2071 2101 2103 2133 166
Size: 32 83 32 1137 30 2 30 2264
Fragments arranged by size:
2264 1137 201 118 109 83 65 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1268 1268
Size: 4231
KasI G'GCGC_C
Cuts at: 1659 1907 1659
Size: 248 3983
KpnI G_GTAC'C
Cuts at: 1249 1249
Size: 4231
MluI A'CGCG_T
Cuts at: 3664 3664
Size: 4231
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 659 2902 3086 659
Size: 2243 184 1804
Fragments arranged by size:
2243 1804 184
MscI TGG'CCA
Cuts at: 711 1990 711
Size: 1279 2952
MslI CAynn'nnrTG
Cuts at: 2218 2345 2218
Size: 127 4104
MspA1I CmG'CkG
Cuts at: 1196 1340 1654 2014 3029 3274 1196
Size: 144 314 360 1015 245 2153
Fragments arranged by size:
2153 1015 360 314 245 144
NaeI GCC'GGC
Cuts at: 1057 2410 1057
Size: 1353 2878
NarI GG'CG_CC
Cuts at: 1660 1908 1660
Size: 248 3983
NcoI C'CATG_G
Cuts at: 125 125
Size: 4231
NgoMIV G'CCGG_C
Cuts at: 1055 2408 1055
Size: 1353 2878
NruI TCG'CGA
Cuts at: 2592 2592
Size: 4231
NspI r_CATG'y
Cuts at: 583 1282 1782 2691 583
Size: 699 500 909 2123
Fragments arranged by size:
2123 909 699 500
PasI CC'CwG_GG
Cuts at: 495 495
Size: 4231
PciI A'CATG_T
Cuts at: 2687 2687
Size: 4231
PflMI CCAn_nnn'nTGG
Cuts at: 561 561
Size: 4231
PmeI GTTT'AAAC
Cuts at: 1228 1228
Size: 4231
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3370 3402 3370
Size: 32 4199
PpuMI rG'GwC_Cy
Cuts at: 237 237
Size: 4231
PspOMI G'GGCC_C
Cuts at: 759 1080 759
Size: 321 3910
PstI C_TGCA'G
Cuts at: 1276 1961 1276
Size: 685 3546
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4231
PvuII CAG'CTG
Cuts at: 1654 2014 1654
Size: 360 3871
RsrII CG'GwC_CG
Cuts at: 2424 2424
Size: 4231
SacI G_AGCT'C
Cuts at: 1243 1243
Size: 4231
SalI G'TCGA_C
Cuts at: 1266 1266
Size: 4231
SapI GCTCTTCn'nnn_
Cuts at: 1160 2252 2462 1160
Size: 1092 210 2929
Fragments arranged by size:
2929 1092 210
SbfI CC_TGCA'GG
Cuts at: 1276 1276
Size: 4231
ScaI AGT'ACT
Cuts at: 3748 3748
Size: 4231
SexAI A'CCwGG_T
Cuts at: 998 998
Size: 4231
SfcI C'TryA_G
Cuts at: 33 1272 1957 2952 3143 33
Size: 1239 685 995 191 1121
Fragments arranged by size:
1239 1121 995 685 191
SfoI GGC'GCC
Cuts at: 1661 1909 1661
Size: 248 3983
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4231
SmaI CCC'GGG
Cuts at: 1251 1251
Size: 4231
SmlI C'TyrA_G
Cuts at: 1099 1384 2299 2793 3055 1099
Size: 285 915 494 262 2275
Fragments arranged by size:
2275 915 494 285 262
SnaBI TAC'GTA
Cuts at: 1221 1221
Size: 4231
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4231
SphI G_CATG'C
Cuts at: 1282 1782 1282
Size: 500 3731
StuI AGG'CCT
Cuts at: 832 1141 832
Size: 309 3922
StyI C'CwwG_G
Cuts at: 125 1363 1717 125
Size: 1238 354 2639
Fragments arranged by size:
2639 1238 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1812 1945 2404 2615 1812
Size: 133 459 211 3428
Fragments arranged by size:
3428 459 211 133
TatI w'GTAC_w
Cuts at: 3746 3746
Size: 4231
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 150 182 3748 3780 150
Size: 32 3566 32 601
Fragments arranged by size:
3566 601 32 32
Tth111I GACn'n_nGTC
Cuts at: 1000 2026 1000
Size: 1026 3205
XbaI T'CTAG_A
Cuts at: 453 1260 453
Size: 807 3424
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1724 1724
Size: 4231
XmaI C'CCGG_G
Cuts at: 1249 1249
Size: 4231
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PasI PciI PflMI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BcgI BfrBI BmgBI BmtI BsiWI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NsiI PacI PfoI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SrfI SspI SwaI XhoI XmnI ZraI