Restriction map for
pF1KSDA0865
(Circular) (Six-base) MAPSORT of: pF1KSDA0865.seq Check: 2654 from: 1 to: 8711
pF1KSDA0865 8711 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1678 3632 4636 1678
Size: 1954 1004 5753
Fragments arranged by size:
5753 1954 1004
AccI GT'mk_AC
Cuts at: 3459 5749 3459
Size: 2290 6421
Acc65I G'GTAC_C
Cuts at: 5727 5727
Size: 8711
AclI AA'CG_TT
Cuts at: 8551 8551
Size: 8711
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 385 694 1658 1786 1961 2070 3202 3613
Size: 96 309 964 128 175 109 1132 411
Cuts at: 3613 3722 5411 5659 6536 6968 7715 7983 289
Size: 109 1689 248 877 432 747 268 1017
Fragments arranged by size:
1689 1132 1017 964 877 747 432 411
309 268 248 175 128 109 109 96
AfeI AGC'GCT
Cuts at: 1396 1396
Size: 8711
AflIII A'CryG_T
Cuts at: 1716 3296 4547 6063 7167 8144 1716
Size: 1580 1251 1516 1104 977 2283
Fragments arranged by size:
2283 1580 1516 1251 1104 977
AgeI A'CCGG_T
Cuts at: 1982 7099 1982
Size: 5117 3594
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 5686 5686
Size: 8711
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1506 1538 2095 2127 3347 3379 1506
Size: 32 557 32 1220 32 6838
Fragments arranged by size:
6838 1220 557 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2205 3624 4892 5580 6016 7583 2205
Size: 1419 1268 688 436 1567 3333
Fragments arranged by size:
3333 1567 1419 1268 688 436
ApaI G_GGCC'C
Cuts at: 4538 5009 4538
Size: 471 8240
ApaLI G'TGCA_C
Cuts at: 1976 3225 7481 1976
Size: 1249 4256 3206
Fragments arranged by size:
4256 3206 1249
ApoI r'AATT_y
Cuts at: 455 1841 2394 2491 2718 3016 5318 5367 5715
Size: 1386 553 97 227 298 2302 49 348
Cuts at: 5715 8064 8320 455
Size: 2349 256 846
Fragments arranged by size:
2349 2302 1386 846 553 348 298 256
227 97 49
AseI AT'TA_AT
Cuts at: 20 20
Size: 8711
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8711
AvaI C'yCGr_G
Cuts at: 399 1300 3749 4237 4263 4291 4405 4458 4864
Size: 901 2449 488 26 28 114 53 406
Cuts at: 4864 5166 5731 399
Size: 302 565 3379
Fragments arranged by size:
3379 2449 901 565 488 406 302 114
53 28 26
BamHI G'GATC_C
Cuts at: 2986 5736 2986
Size: 2750 5961
BanI G'GyrC_C
Cuts at: 1681 4258 4323 4962 5601 5727 6141 6389 6424
Size: 2577 65 639 639 126 414 248 35
Cuts at: 6424 1681
Size: 3968
Fragments arranged by size:
3968 2577 639 639 414 248 126 65
35
BanII G_rGCy'C
Cuts at: 1112 1361 2070 4471 4538 4735 4922 5009 5150
Size: 249 709 2401 67 197 187 87 141
Cuts at: 5150 5597 5684 5725 6755 1112
Size: 447 87 41 1030 3068
Fragments arranged by size:
3068 2401 1030 709 447 249 197 187
141 87 87 67 41
BbeI G_GCGC'C
Cuts at: 1685 4262 4327 4966 6145 6393 1685
Size: 2577 65 639 1179 248 4003
Fragments arranged by size:
4003 2577 1179 639 248 65
BbsI GAAGACnn'nnnn_
Cuts at: 503 503
Size: 8711
BbvCI CC'TCA_GC
Cuts at: 5488 5488
Size: 8711
BciVI GTATCCnnnnn_n'
Cuts at: 3349 5395 6609 7370 8711 3349
Size: 2046 1214 761 1341 3349
Fragments arranged by size:
3349 2046 1341 1214 761
BclI T'GATC_A
Cuts at: 882 2948 6231 882
Size: 2066 3283 3362
Fragments arranged by size:
3362 3283 2066
BfrBI ATG'CAT
Cuts at: 954 2014 954
Size: 1060 7651
BglI GCCn_nnn'nGGC
Cuts at: 1690 3929 4267 4590 4766 4887 4999 5056 5071
Size: 2239 338 323 176 121 112 57 15
Cuts at: 5071 1690
Size: 5330
Fragments arranged by size:
5330 2239 338 323 176 121 112 57
15
BglII A'GATC_T
Cuts at: 1412 3341 6226 8263 1412
Size: 1929 2885 2037 1860
Fragments arranged by size:
2885 2037 1929 1860
BlpI GC'TnA_GC
Cuts at: 1351 3305 4201 5591 5823 1351
Size: 1954 896 1390 232 4239
Fragments arranged by size:
4239 1954 1390 896 232
Bme1580I G_kGCm'C
Cuts at: 1980 3229 4379 4538 5009 6336 6429 7485 1980
Size: 1249 1150 159 471 1327 93 1056 3206
Fragments arranged by size:
3206 1327 1249 1150 1056 471 159 93
BmrI ACTGGGnnnn_n'
Cuts at: 5923 6004 6339 8483 5923
Size: 81 335 2144 6151
Fragments arranged by size:
6151 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2193 7078 2193
Size: 4885 3826
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 590 779 2182 3349 3454 4687 4711 5064
Size: 19 189 1403 1167 105 1233 24 353
Cuts at: 5064 5103 5556 571
Size: 39 453 3726
Fragments arranged by size:
3726 1403 1233 1167 453 353 189 105
39 24 19
Bpu10I CC'TnA_GC
Cuts at: 1095 5488 1095
Size: 4393 4318
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1085 4687 5276 5887 6766 7258 7556 1085
Size: 3602 589 611 879 492 298 2240
Fragments arranged by size:
3602 2240 879 611 589 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 708 857 2330 3950 4687 8415 708
Size: 149 1473 1620 737 3728 1004
Fragments arranged by size:
3728 1620 1473 1004 737 149
BsaAI yAC'GTr
Cuts at: 3297 5703 6064 3297
Size: 2406 361 5944
Fragments arranged by size:
5944 2406 361
BsaBI GATnn'nnATC
Cuts at: 6 6225 8262 6
Size: 6219 2037 455
Fragments arranged by size:
6219 2037 455
BsaHI Gr'CG_yC
Cuts at: 508 997 1682 4259 4324 4963 5063 6142 6390
Size: 489 685 2577 65 639 100 1079 248
Cuts at: 6390 7092 8588 508
Size: 702 1496 631
Fragments arranged by size:
2577 1496 1079 702 685 639 631 489
248 100 65
BsaWI w'CCGG_w
Cuts at: 1831 1982 5900 6421 7099 7373 7520 7931 1831
Size: 151 3918 521 678 274 147 411 2611
Fragments arranged by size:
3918 2611 678 521 411 274 151 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 464 494 563 593 752 782 823 853 982
Size: 30 69 30 159 30 41 30 129
Cuts at: 982 1012 1531 1561 2095 2125 2726 2756 3149
Size: 30 519 30 534 30 601 30 393
Cuts at: 3149 3179 3452 3482 3865 3895 4402 4417 4432
Size: 30 273 30 383 30 507 15 15
Cuts at: 4432 4447 464
Size: 15 4728
Fragments arranged by size:
4728 601 534 519 507 393 383 273
159 129 69 41 30 30 30 30
30 30 30 30 30 30 30 15
15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 387 485 1003 1497 1526 3170 4157 4231 4438
Size: 98 518 494 29 1644 987 74 207
Cuts at: 4438 5187 5553 387
Size: 749 366 3545
Fragments arranged by size:
3545 1644 987 749 518 494 366 207
98 74 29
BseYI C'CCAG_C
Cuts at: 1490 3877 4371 5113 5629 6136 6894 7471 7967
Size: 2387 494 742 516 507 758 577 496
Cuts at: 7967 1490
Size: 2234
Fragments arranged by size:
2387 2234 758 742 577 516 507 496
494
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3940 4735 5566 3940
Size: 795 831 7085
Fragments arranged by size:
7085 831 795
BsiEI CG_ry'CG
Cuts at: 104 3902 4594 4821 6253 6299 7507 104
Size: 3798 692 227 1432 46 1208 1308
Fragments arranged by size:
3798 1432 1308 1208 692 227 46
BsiHKAI G_wGCw'C
Cuts at: 429 1361 1980 3229 5150 5684 5725 6503 6693
Size: 932 619 1249 1921 534 41 778 190
Cuts at: 6693 7485 429
Size: 792 1655
Fragments arranged by size:
1921 1655 1249 932 792 778 619 534
190 41
BsmI GAATG_Cn'
Cuts at: 3738 3738
Size: 8711
BsmBI CGTCTCn'nnnn_
Cuts at: 399 4001 399
Size: 3602 5109
Bsp1286I G_dGCh'C
Cuts at: 429 1112 1361 1980 2070 3229 4379 4471 4538
Size: 683 249 619 90 1159 1150 92 67
Cuts at: 4538 4735 4922 5009 5150 5597 5684 5725 6336
Size: 197 187 87 141 447 87 41 611
Cuts at: 6336 6429 6503 6693 6755 7485 429
Size: 93 74 190 62 730 1655
Fragments arranged by size:
1655 1159 1150 730 683 619 611 447
249 197 190 187 141 93 92 90
87 87 74 67 62 41
BspEI T'CCGG_A
Cuts at: 7931 7931
Size: 8711
BspHI T'CATG_A
Cuts at: 7887 7887
Size: 8711
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1296 1678 3592 3632 4586 4636 5761 5975 6277
Size: 382 1914 40 954 50 1125 214 302
Cuts at: 6277 6658 1296
Size: 381 3349
Fragments arranged by size:
3349 1914 1125 954 382 381 302 214
50 40
BsrBI CCG'CTC
Cuts at: 50 1286 7003 7057 8541 8706 50
Size: 1236 5717 54 1484 165 55
Fragments arranged by size:
5717 1484 1236 165 55 54
BsrDI GCAATG_nn'
Cuts at: 446 518 3243 3743 6623 446
Size: 72 2725 500 2880 2534
Fragments arranged by size:
2880 2725 2534 500 72
BsrFI r'CCGG_y
Cuts at: 1982 3761 4589 5074 6709 6890 7099 1982
Size: 1779 828 485 1635 181 209 3594
Fragments arranged by size:
3594 1779 1635 828 485 209 181
BsrGI T'GTAC_A
Cuts at: 2357 2580 4358 2357
Size: 223 1778 6710
Fragments arranged by size:
6710 1778 223
BssHII G'CGCG_C
Cuts at: 4276 4307 4511 4895 4276
Size: 31 204 384 8092
Fragments arranged by size:
8092 384 204 31
BssSI C'ACGA_G
Cuts at: 973 2563 6982 7340 973
Size: 1590 4419 358 2344
Fragments arranged by size:
4419 2344 1590 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2614 5580 6088 2614
Size: 2966 508 5237
Fragments arranged by size:
5237 2966 508
BstBI TT'CG_AA
Cuts at: 110 7072 110
Size: 6962 1749
BstEII G'GTnAC_C
Cuts at: 2620 2620
Size: 8711
BstYI r'GATC_y
Cuts at: 1 1412 2986 3159 3341 5027 5576 5736 6203
Size: 1411 1574 173 182 1686 549 160 467
Cuts at: 6203 6226 6561 6807 7819 7905 7917 8263 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1686 1574 1411 1012 549 467 449 346
335 246 182 173 160 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1509 1509
Size: 8711
BtgI C'CryG_G
Cuts at: 125 1481 2597 2817 3902 4000 4085 4253 4482
Size: 1356 1116 220 1085 98 85 168 229
Cuts at: 4482 4997 5192 5313 125
Size: 515 195 121 3523
Fragments arranged by size:
3523 1356 1116 1085 515 229 220 195
168 121 98 85
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3468 4475 6661 6842 7005 3468
Size: 1007 2186 181 163 5174
Fragments arranged by size:
5174 2186 1007 181 163
BtsI GCAGTG_nn'
Cuts at: 691 3481 4309 5954 691
Size: 2790 828 1645 3448
Fragments arranged by size:
3448 2790 1645 828
DraI TTT'AAA
Cuts at: 2283 3015 5710 2283
Size: 732 2695 5284
Fragments arranged by size:
5284 2695 732
DraIII CAC_nnn'GTG
Cuts at: 975 3963 975
Size: 2988 5723
DrdI GACnn_nn'nnGTC
Cuts at: 472 6417 7275 472
Size: 5945 858 1908
Fragments arranged by size:
5945 1908 858
EaeI y'GGCC_r
Cuts at: 165 921 1890 2345 3899 4088 4100 4382 4591
Size: 756 969 455 1554 189 12 282 209
Cuts at: 4591 4818 5000 6296 6470 6861 6888 8233 165
Size: 227 182 1296 174 391 27 1345 643
Fragments arranged by size:
1554 1345 1296 969 756 643 455 391
282 227 209 189 182 174 27 12
EagI C'GGCC_G
Cuts at: 3899 4591 4818 6296 3899
Size: 692 227 1478 6314
Fragments arranged by size:
6314 1478 692 227
EarI CTCTTCn'nnn_
Cuts at: 1100 1112 1549 1582 2468 5164 6734 6944 1100
Size: 12 437 33 886 2696 1570 210 2867
Fragments arranged by size:
2867 2696 1570 886 437 210 33 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2169 5074 6919 7229 7375 8521 2169
Size: 2905 1845 310 146 1146 2359
Fragments arranged by size:
2905 2359 1845 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1359 5148 5682 5723 1359
Size: 3789 534 41 4347
Fragments arranged by size:
4347 3789 534 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 385 571 590 694 779 1658 1786 1961
Size: 96 186 19 104 85 879 128 175
Cuts at: 1961 2070 2182 3202 3349 3454 3613 3722 4687
Size: 109 112 1020 147 105 159 109 965
Cuts at: 4687 4711 5064 5103 5411 5556 5659 6536 6968
Size: 24 353 39 308 145 103 877 432
Cuts at: 6968 7715 7983 289
Size: 747 268 1017
Fragments arranged by size:
1020 1017 965 879 877 747 432 353
308 268 186 175 159 147 145 128
112 109 109 105 104 103 96 85
39 24 19
EcoNI CCTnn'n_nnAGG
Cuts at: 367 586 2167 5664 367
Size: 219 1581 3497 3414
Fragments arranged by size:
3497 3414 1581 219
EcoO109I rG'GnC_Cy
Cuts at: 407 3324 4032 4229 4534 4535 4889 5006 5654
Size: 2917 708 197 305 1 354 117 648
Cuts at: 5654 5850 407
Size: 196 3268
Fragments arranged by size:
3268 2917 708 648 354 305 197 196
117 1
EcoRI G'AATT_C
Cuts at: 2394 5318 5715 2394
Size: 2924 397 5390
Fragments arranged by size:
5390 2924 397
EcoRV GAT'ATC
Cuts at: 4223 4223
Size: 8711
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 370 2917 6730 370
Size: 2547 3813 2351
Fragments arranged by size:
3813 2547 2351
FspI TGC'GCA
Cuts at: 6492 6492
Size: 8711
HaeII r_GCGC'y
Cuts at: 1398 1685 4076 4262 4327 4804 4966 6145 6393
Size: 287 2391 186 65 477 162 1179 248
Cuts at: 6393 7415 8394 1398
Size: 1022 979 1715
Fragments arranged by size:
2391 1715 1179 1022 979 477 287 248
186 162 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 464 496 563 595 673 705 752 784 823
Size: 32 67 32 78 32 47 32 39
Cuts at: 823 855 982 1014 1066 1098 1918 1950 1999
Size: 32 127 32 52 32 820 32 49
Cuts at: 1999 2031 3149 3181 3373 3405 3452 3484 4152
Size: 32 1118 32 192 32 47 32 668
Cuts at: 4152 4184 4207 4239 5389 5421 6553 6583 6585
Size: 32 23 32 1150 32 1132 30 2
Cuts at: 6585 6615 464
Size: 30 2560
Fragments arranged by size:
2560 1150 1132 1118 820 668 192 127
78 67 52 49 47 47 39 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 30 30
23 2
HincII GTy'rAC
Cuts at: 477 483 656 731 1449 2511 3460 5750 477
Size: 6 173 75 718 1062 949 2290 3438
Fragments arranged by size:
3438 2290 1062 949 718 173 75 6
HindIII A'AGCT_T
Cuts at: 706 706
Size: 8711
HpaI GTT'AAC
Cuts at: 731 1449 731
Size: 718 7993
KasI G'GCGC_C
Cuts at: 1681 4258 4323 4962 6141 6389 1681
Size: 2577 65 639 1179 248 4003
Fragments arranged by size:
4003 2577 1179 639 248 65
KpnI G_GTAC'C
Cuts at: 5731 5731
Size: 8711
MfeI C'AATT_G
Cuts at: 1175 1175
Size: 8711
MluI A'CGCG_T
Cuts at: 4547 8144 4547
Size: 3597 5114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1178 4667 7382 7566 1178
Size: 3489 2715 184 2323
Fragments arranged by size:
3489 2715 2323 184
MscI TGG'CCA
Cuts at: 167 923 1892 2347 6472 167
Size: 756 969 455 4125 2406
Fragments arranged by size:
4125 2406 969 756 455
MslI CAynn'nnrTG
Cuts at: 310 338 422 491 2836 4366 6700 6827 310
Size: 28 84 69 2345 1530 2334 127 2194
Fragments arranged by size:
2345 2334 2194 1530 127 84 69 28
MspA1I CmG'CkG
Cuts at: 1494 1590 3445 4087 4205 4255 4737 5194 5487
Size: 96 1855 642 118 50 482 457 293
Cuts at: 5487 5822 6136 6496 7509 7754 1494
Size: 335 314 360 1013 245 2451
Fragments arranged by size:
2451 1855 1013 642 482 457 360 335
314 293 245 118 96 50
NaeI GCC'GGC
Cuts at: 5076 6892 5076
Size: 1816 6895
NarI GG'CG_CC
Cuts at: 1682 4259 4324 4963 6142 6390 1682
Size: 2577 65 639 1179 248 4003
Fragments arranged by size:
4003 2577 1179 639 248 65
NcoI C'CATG_G
Cuts at: 125 1481 2597 5313 125
Size: 1356 1116 2716 3523
Fragments arranged by size:
3523 2716 1356 1116
NgoMIV G'CCGG_C
Cuts at: 5074 6890 5074
Size: 1816 6895
NsiI A_TGCA'T
Cuts at: 956 2016 956
Size: 1060 7651
NspI r_CATG'y
Cuts at: 195 277 315 1720 1750 2433 3535 3822 5764
Size: 82 38 1405 30 683 1102 287 1942
Cuts at: 5764 6264 7171 195
Size: 500 907 1735
Fragments arranged by size:
1942 1735 1405 1102 907 683 500 287
82 38 30
PasI CC'CwG_GG
Cuts at: 4540 5597 4540
Size: 1057 7654
PciI A'CATG_T
Cuts at: 1716 7167 1716
Size: 5451 3260
PflMI CCAn_nnn'nTGG
Cuts at: 2205 2205
Size: 8711
PfoI T'CCnGG_A
Cuts at: 587 848 1580 2204 3326 4688 587
Size: 261 732 624 1122 1362 4610
Fragments arranged by size:
4610 1362 1122 732 624 261
PmeI GTTT'AAAC
Cuts at: 5710 5710
Size: 8711
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2095 2127 7850 7882 2095
Size: 32 5723 32 2924
Fragments arranged by size:
5723 2924 32 32
PpuMI rG'GwC_Cy
Cuts at: 407 3324 4032 4229 407
Size: 2917 708 197 4889
Fragments arranged by size:
4889 2917 708 197
PshAI GACnn'nnGTC
Cuts at: 3030 4621 3030
Size: 1591 7120
PsiI TTA'TAA
Cuts at: 3580 3580
Size: 8711
PspOMI G'GGCC_C
Cuts at: 4534 5005 4534
Size: 471 8240
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3772 3804 3772
Size: 32 8679
PstI C_TGCA'G
Cuts at: 1675 3683 5014 5522 5758 6443 1675
Size: 2008 1331 508 236 685 3943
Fragments arranged by size:
3943 2008 1331 685 508 236
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8711
PvuII CAG'CTG
Cuts at: 1494 3445 5487 6136 6496 1494
Size: 1951 2042 649 360 3709
Fragments arranged by size:
3709 2042 1951 649 360
RsrII CG'GwC_CG
Cuts at: 6906 6906
Size: 8711
SacI G_AGCT'C
Cuts at: 1361 5150 5684 5725 1361
Size: 3789 534 41 4347
Fragments arranged by size:
4347 3789 534 41
SacII CC_GC'GG
Cuts at: 4088 4256 5195 4088
Size: 168 939 7604
Fragments arranged by size:
7604 939 168
SalI G'TCGA_C
Cuts at: 3458 5748 3458
Size: 2290 6421
SanDI GG'GwC_CC
Cuts at: 407 4032 4229 407
Size: 3625 197 4889
Fragments arranged by size:
4889 3625 197
SapI GCTCTTCn'nnn_
Cuts at: 1100 1582 6734 6944 1100
Size: 482 5152 210 2867
Fragments arranged by size:
5152 2867 482 210
SbfI CC_TGCA'GG
Cuts at: 1675 5522 5758 1675
Size: 3847 236 4628
Fragments arranged by size:
4628 3847 236
ScaI AGT'ACT
Cuts at: 889 4750 8228 889
Size: 3861 3478 1372
Fragments arranged by size:
3861 3478 1372
SfcI C'TryA_G
Cuts at: 33 316 623 1671 3559 3679 4143 4884 5010
Size: 283 307 1048 1888 120 464 741 126
Cuts at: 5010 5103 5518 5754 6439 7432 7623 33
Size: 93 415 236 685 993 191 1121
Fragments arranged by size:
1888 1121 1048 993 741 685 464 415
307 283 236 191 126 120 93
SfoI GGC'GCC
Cuts at: 1683 4260 4325 4964 6143 6391 1683
Size: 2577 65 639 1179 248 4003
Fragments arranged by size:
4003 2577 1179 639 248 65
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8711
SmaI CCC'GGG
Cuts at: 3751 4239 4265 4293 4407 4460 4866 5733 3751
Size: 488 26 28 114 53 406 867 6729
Fragments arranged by size:
6729 867 488 406 114 53 28 26
SmlI C'TyrA_G
Cuts at: 1100 4702 5291 5866 6781 7273 7535 1100
Size: 3602 589 575 915 492 262 2276
Fragments arranged by size:
3602 2276 915 589 575 492 262
SnaBI TAC'GTA
Cuts at: 5703 5703
Size: 8711
SpeI A'CTAG_T
Cuts at: 67 2932 67
Size: 2865 5846
SphI G_CATG'C
Cuts at: 195 277 2433 3535 5764 6264 195
Size: 82 2156 1102 2229 500 2642
Fragments arranged by size:
2642 2229 2156 1102 500 82
SrfI GCCC'GGGC
Cuts at: 4460 4866 4460
Size: 406 8305
SspI AAT'ATT
Cuts at: 2132 2278 2453 2132
Size: 146 175 8390
Fragments arranged by size:
8390 175 146
StuI AGG'CCT
Cuts at: 5124 5124
Size: 8711
StyI C'CwwG_G
Cuts at: 125 162 1481 2139 2597 4967 5313 5845 6199
Size: 37 1319 658 458 2370 346 532 354
Cuts at: 6199 125
Size: 2637
Fragments arranged by size:
2637 2370 1319 658 532 458 354 346
37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4991 6294 6427 6886 7095 4991
Size: 1303 133 459 209 6607
Fragments arranged by size:
6607 1303 459 209 133
TatI w'GTAC_w
Cuts at: 887 2357 2580 2771 4358 4748 8226 887
Size: 1470 223 191 1587 390 3478 1372
Fragments arranged by size:
3478 1587 1470 1372 390 223 191
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1531 1563 3865 3897 4417 4449 8228 8260 1531
Size: 32 2302 32 520 32 3779 32 1982
Fragments arranged by size:
3779 2302 1982 520 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6508 6508
Size: 8711
XbaI T'CTAG_A
Cuts at: 3093 5742 3093
Size: 2649 6062
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 595 2659 3925 4110 6206 595
Size: 2064 1266 185 2096 3100
Fragments arranged by size:
3100 2096 2064 1266 185
XmaI C'CCGG_G
Cuts at: 3749 4237 4263 4291 4405 4458 4864 5731 3749
Size: 488 26 28 114 53 406 867 6729
Fragments arranged by size:
6729 867 488 406 114 53 28 26
XmnI GAAnn'nnTTC
Cuts at: 353 2110 2477 3343 353
Size: 1757 367 866 5721
Fragments arranged by size:
5721 1757 866 367
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AscI AvrII BaeI BcgI
BmgBI BmtI BsiWI BstXI BstZ17I ClaI CspCI FseI
FspAI NdeI NheI NotI NruI PacI PmlI PspXI
SexAI SfiI SgrAI SwaI XhoI ZraI