Restriction map for
pF1KSDA0860
(Circular) (Six-base) MAPSORT of: pF1KSDA0860.seq Check: 691 from: 1 to: 4762
pF1KSDA0860 4762 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1157 1157
Size: 4762
AccI GT'mk_AC
Cuts at: 1798 1798
Size: 4762
Acc65I G'GTAC_C
Cuts at: 1776 1776
Size: 4762
AclI AA'CG_TT
Cuts at: 4602 4602
Size: 4762
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 2585 3017 3766 4034 239
Size: 2346 432 749 268 967
Fragments arranged by size:
2346 967 749 432 268
AflII C'TTAA_G
Cuts at: 642 642
Size: 4762
AflIII A'CryG_T
Cuts at: 1236 2112 3218 4195 1236
Size: 876 1106 977 1803
Fragments arranged by size:
1803 1106 977 876
AgeI A'CCGG_T
Cuts at: 3150 3150
Size: 4762
AleI CACnn'nnGTG
Cuts at: 661 661
Size: 4762
AlwNI CAG_nnn'CTG
Cuts at: 879 1080 1404 2065 3634 879
Size: 201 324 661 1569 2007
Fragments arranged by size:
2007 1569 661 324 201
ApaI G_GGCC'C
Cuts at: 436 1567 436
Size: 1131 3631
ApaLI G'TGCA_C
Cuts at: 1695 3532 1695
Size: 1837 2925
ApoI r'AATT_y
Cuts at: 164 1764 4115 4371 164
Size: 1600 2351 256 555
Fragments arranged by size:
2351 1600 555 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4762
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4762
AvaI C'yCGr_G
Cuts at: 1780 1780
Size: 4762
BamHI G'GATC_C
Cuts at: 918 1785 918
Size: 867 3895
BanI G'GyrC_C
Cuts at: 1531 1776 2190 2438 2473 1531
Size: 245 414 248 35 3820
Fragments arranged by size:
3820 414 248 245 35
BanII G_rGCy'C
Cuts at: 436 602 618 1352 1567 1774 2804 436
Size: 166 16 734 215 207 1030 2394
Fragments arranged by size:
2394 1030 734 215 207 166 16
BbeI G_GCGC'C
Cuts at: 2194 2442 2194
Size: 248 4514
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 957 991 957
Size: 34 4728
BciVI GTATCCnnnnn_n'
Cuts at: 688 2658 3421 4762 688
Size: 1970 763 1341 688
Fragments arranged by size:
1970 1341 763 688
BclI T'GATC_A
Cuts at: 2280 2280
Size: 4762
BglI GCCn_nnn'nGGC
Cuts at: 527 527
Size: 4762
BglII A'GATC_T
Cuts at: 222 2275 4314 222
Size: 2053 2039 670
Fragments arranged by size:
2053 2039 670
BlpI GC'TnA_GC
Cuts at: 440 1872 440
Size: 1432 3330
Bme1580I G_kGCm'C
Cuts at: 436 1034 1567 1699 2385 2478 3536 436
Size: 598 533 132 686 93 1058 1662
Fragments arranged by size:
1662 1058 686 598 533 132 93
BmgBI CAC'GTC
Cuts at: 1694 1731 1694
Size: 37 4725
BmrI ACTGGGnnnn_n'
Cuts at: 411 1028 1972 2053 2388 4534 411
Size: 617 944 81 335 2146 639
Fragments arranged by size:
2146 944 639 617 335 81
BmtI G_CTAG'C
Cuts at: 1724 1724
Size: 4762
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3127 3127
Size: 4762
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 785 849 952 1012 1108 1471 1542 785
Size: 64 103 60 96 363 71 4005
Fragments arranged by size:
4005 363 103 96 71 64 60
Bpu10I CC'TnA_GC
Cuts at: 625 625
Size: 4762
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1081 1936 2815 3309 3607 1081
Size: 855 879 494 298 2236
Fragments arranged by size:
2236 879 855 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4466 4466
Size: 4762
BsaAI yAC'GTr
Cuts at: 633 1752 2113 633
Size: 1119 361 3282
Fragments arranged by size:
3282 1119 361
BsaBI GATnn'nnATC
Cuts at: 6 652 2274 4313 6
Size: 646 1622 2039 455
Fragments arranged by size:
2039 1622 646 455
BsaHI Gr'CG_yC
Cuts at: 2191 2439 3143 4639 2191
Size: 248 704 1496 2314
Fragments arranged by size:
2314 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1949 2470 3150 3424 3571 3982 1949
Size: 521 680 274 147 411 2729
Fragments arranged by size:
2729 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1606 1636 1606
Size: 30 4732
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 922 922
Size: 4762
BseYI C'CCAG_C
Cuts at: 340 435 1351 2185 2943 3522 4018 340
Size: 95 916 834 758 579 496 1084
Fragments arranged by size:
1084 916 834 758 579 496 95
BsiEI CG_ry'CG
Cuts at: 104 1714 2302 2348 3558 104
Size: 1610 588 46 1210 1308
Fragments arranged by size:
1610 1308 1210 588 46
BsiHKAI G_wGCw'C
Cuts at: 602 988 1164 1319 1699 1774 2552 2742 3536
Size: 386 176 155 380 75 778 190 794
Cuts at: 3536 602
Size: 1828
Fragments arranged by size:
1828 794 778 386 380 190 176 155
75
Bsp1286I G_dGCh'C
Cuts at: 436 602 618 988 1034 1164 1319 1352 1567
Size: 166 16 370 46 130 155 33 215
Cuts at: 1567 1699 1774 2385 2478 2552 2742 2804 3536
Size: 132 75 611 93 74 190 62 732
Cuts at: 3536 436
Size: 1662
Fragments arranged by size:
1662 732 611 370 215 190 166 155
132 130 93 75 74 62 46 33
16
BspEI T'CCGG_A
Cuts at: 3982 3982
Size: 4762
BspHI T'CATG_A
Cuts at: 3938 3938
Size: 4762
BspMI ACCTGCnnnn'nnnn_
Cuts at: 733 782 1157 1810 2024 2326 2707 733
Size: 49 375 653 214 302 381 2788
Fragments arranged by size:
2788 653 381 375 302 214 49
BsrBI CCG'CTC
Cuts at: 50 3052 3106 4592 4757 50
Size: 3002 54 1486 165 55
Fragments arranged by size:
3002 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2672 2672
Size: 4762
BsrFI r'CCGG_y
Cuts at: 714 1534 1617 1713 2758 2939 3150 714
Size: 820 83 96 1045 181 211 2326
Fragments arranged by size:
2326 1045 820 211 181 96 83
BsrGI T'GTAC_A
Cuts at: 374 374
Size: 4762
BssSI C'ACGA_G
Cuts at: 1393 3031 3391 1393
Size: 1638 360 2764
Fragments arranged by size:
2764 1638 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1703 2137 1703
Size: 434 4328
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4762
BstEII G'GTnAC_C
Cuts at: 759 759
Size: 4762
BstXI CCAn_nnnn'nTGG
Cuts at: 930 1153 1495 930
Size: 223 342 4197
Fragments arranged by size:
4197 342 223
BstYI r'GATC_y
Cuts at: 1 222 647 918 1785 2252 2275 2610 2856
Size: 221 425 271 867 467 23 335 246
Cuts at: 2856 3870 3956 3968 4314 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 867 467 449 425 346 335 271
246 221 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 779 905 963 779
Size: 126 58 4578
Fragments arranged by size:
4578 126 58
BtgI C'CryG_G
Cuts at: 125 812 125
Size: 687 4075
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 571 2710 2891 3054 571
Size: 2139 181 163 2279
Fragments arranged by size:
2279 2139 181 163
BtsI GCAGTG_nn'
Cuts at: 168 1014 1184 1319 2003 168
Size: 846 170 135 684 2927
Fragments arranged by size:
2927 846 684 170 135
DraI TTT'AAA
Cuts at: 1759 1759
Size: 4762
DrdI GACnn_nn'nnGTC
Cuts at: 2466 3326 2466
Size: 860 3902
EaeI y'GGCC_r
Cuts at: 716 881 1638 1711 2345 2519 2910 2937 4284
Size: 165 757 73 634 174 391 27 1347
Cuts at: 4284 716
Size: 1194
Fragments arranged by size:
1347 1194 757 634 391 174 165 73
27
EagI C'GGCC_G
Cuts at: 1711 2345 1711
Size: 634 4128
EarI CTCTTCn'nnn_
Cuts at: 2783 2993 2783
Size: 210 4552
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2968 3280 3426 4572 2968
Size: 312 146 1146 3158
Fragments arranged by size:
3158 1146 312 146
EcoICRI GAG'CTC
Cuts at: 600 1772 600
Size: 1172 3590
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 785 849 952 1012 1108 1471 1542 2585
Size: 546 64 103 60 96 363 71 1043
Cuts at: 2585 3017 3766 4034 239
Size: 432 749 268 967
Fragments arranged by size:
1043 967 749 546 432 363 268 103
96 71 64 60
EcoNI CCTnn'n_nnAGG
Cuts at: 778 778
Size: 4762
EcoO109I rG'GnC_Cy
Cuts at: 542 1564 1899 542
Size: 1022 335 3405
Fragments arranged by size:
3405 1022 335
EcoRI G'AATT_C
Cuts at: 164 1764 164
Size: 1600 3162
EcoRV GAT'ATC
Cuts at: 182 182
Size: 4762
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 634 2779 634
Size: 2145 2617
FspI TGC'GCA
Cuts at: 2541 2541
Size: 4762
HaeII r_GCGC'y
Cuts at: 2194 2442 3466 4445 2194
Size: 248 1024 979 2511
Fragments arranged by size:
2511 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2602 2632 2634 2664 2602
Size: 30 2 30 4700
Fragments arranged by size:
4700 30 30 2
HincII GTy'rAC
Cuts at: 1799 1799
Size: 4762
HindIII A'AGCT_T
Cuts at: 1413 1413
Size: 4762
KasI G'GCGC_C
Cuts at: 2190 2438 2190
Size: 248 4514
KpnI G_GTAC'C
Cuts at: 1780 1780
Size: 4762
MluI A'CGCG_T
Cuts at: 4195 4195
Size: 4762
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 677 3433 3617 677
Size: 2756 184 1822
Fragments arranged by size:
2756 1822 184
MscI TGG'CCA
Cuts at: 2521 2521
Size: 4762
MslI CAynn'nnrTG
Cuts at: 661 2749 2876 661
Size: 2088 127 2547
Fragments arranged by size:
2547 2088 127
MspA1I CmG'CkG
Cuts at: 187 340 439 1629 1710 1871 2185 2545 3560
Size: 153 99 1190 81 161 314 360 1015
Cuts at: 3560 3805 187
Size: 245 1144
Fragments arranged by size:
1190 1144 1015 360 314 245 161 153
99 81
NaeI GCC'GGC
Cuts at: 716 2941 716
Size: 2225 2537
NarI GG'CG_CC
Cuts at: 2191 2439 2191
Size: 248 4514
NcoI C'CATG_G
Cuts at: 125 812 125
Size: 687 4075
NdeI CA'TA_TG
Cuts at: 1364 1364
Size: 4762
NgoMIV G'CCGG_C
Cuts at: 714 2939 714
Size: 2225 2537
NheI G'CTAG_C
Cuts at: 1720 1720
Size: 4762
NruI TCG'CGA
Cuts at: 3123 3123
Size: 4762
NspI r_CATG'y
Cuts at: 1240 1813 2313 3222 1240
Size: 573 500 909 2780
Fragments arranged by size:
2780 909 573 500
PasI CC'CwG_GG
Cuts at: 429 832 429
Size: 403 4359
PciI A'CATG_T
Cuts at: 1236 3218 1236
Size: 1982 2780
PfoI T'CCnGG_A
Cuts at: 914 1519 914
Size: 605 4157
PmeI GTTT'AAAC
Cuts at: 1759 1759
Size: 4762
PmlI CAC'GTG
Cuts at: 633 633
Size: 4762
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3901 3933 3901
Size: 32 4730
PspOMI G'GGCC_C
Cuts at: 432 1563 432
Size: 1131 3631
PstI C_TGCA'G
Cuts at: 797 875 1807 2492 797
Size: 78 932 685 3067
Fragments arranged by size:
3067 932 685 78
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4762
PvuII CAG'CTG
Cuts at: 187 340 439 1629 2185 2545 187
Size: 153 99 1190 556 360 2404
Fragments arranged by size:
2404 1190 556 360 153 99
RsrII CG'GwC_CG
Cuts at: 2955 2955
Size: 4762
SacI G_AGCT'C
Cuts at: 602 1774 602
Size: 1172 3590
SalI G'TCGA_C
Cuts at: 1797 1797
Size: 4762
SapI GCTCTTCn'nnn_
Cuts at: 2783 2993 2783
Size: 210 4552
SbfI CC_TGCA'GG
Cuts at: 1807 1807
Size: 4762
ScaI AGT'ACT
Cuts at: 4279 4279
Size: 4762
SfcI C'TryA_G
Cuts at: 33 315 793 871 1803 2488 3483 3674 33
Size: 282 478 78 932 685 995 191 1121
Fragments arranged by size:
1121 995 932 685 478 282 191 78
SfoI GGC'GCC
Cuts at: 2192 2440 2192
Size: 248 4514
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4762
SmaI CCC'GGG
Cuts at: 1782 1782
Size: 4762
SmlI C'TyrA_G
Cuts at: 642 1096 1915 2830 3324 3586 642
Size: 454 819 915 494 262 1818
Fragments arranged by size:
1818 915 819 494 454 262
SnaBI TAC'GTA
Cuts at: 1752 1752
Size: 4762
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4762
SphI G_CATG'C
Cuts at: 1813 2313 1813
Size: 500 4262
StuI AGG'CCT
Cuts at: 1527 1527
Size: 4762
StyI C'CwwG_G
Cuts at: 125 473 812 1427 1440 1494 1894 2248 125
Size: 348 339 615 13 54 400 354 2639
Fragments arranged by size:
2639 615 400 354 348 339 54 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 673 1654 2343 2476 2935 3146 673
Size: 981 689 133 459 211 2289
Fragments arranged by size:
2289 981 689 459 211 133
TatI w'GTAC_w
Cuts at: 374 4277 374
Size: 3903 859
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4279 4311 4279
Size: 32 4730
Tth111I GACn'n_nGTC
Cuts at: 2557 2557
Size: 4762
XbaI T'CTAG_A
Cuts at: 1791 1791
Size: 4762
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1290 2255 1290
Size: 965 3797
XmaI C'CCGG_G
Cuts at: 1780 1780
Size: 4762
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NruI NspI PasI
PciI PfoI PmeI PmlI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI AscI AvrII BaeI
BbsI BbvCI BfrBI BsgI BsiWI BsmI BsmBI BssHII
BstZ17I ClaI CspCI DraIII FseI FspAI HpaI MfeI
NotI NsiI PacI PflMI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI
SwaI XhoI XmnI ZraI