Restriction map for
pF1KSDA0859
(Circular) (Six-base) MAPSORT of: pF1KSDA0859.seq Check: 8789 from: 1 to: 5236
pF1KSDA0859 5236 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 567 567
Size: 5236
AccI GT'mk_AC
Cuts at: 1362 2272 1362
Size: 910 4326
Acc65I G'GTAC_C
Cuts at: 2250 2250
Size: 5236
AclI AA'CG_TT
Cuts at: 5076 5076
Size: 5236
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 769 1098 1435 1967 3059 3491 4240 4508
Size: 123 329 337 532 1092 432 749 268
Cuts at: 4508 646
Size: 1374
Fragments arranged by size:
1374 1092 749 532 432 337 329 268
123
AflIII A'CryG_T
Cuts at: 2180 2586 3692 4669 2180
Size: 406 1106 977 2747
Fragments arranged by size:
2747 1106 977 406
AgeI A'CCGG_T
Cuts at: 3624 3624
Size: 5236
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 841 916 1490 1842 2120 841
Size: 75 574 352 278 3957
Fragments arranged by size:
3957 574 352 278 75
AlwNI CAG_nnn'CTG
Cuts at: 772 2539 4108 772
Size: 1767 1569 1900
Fragments arranged by size:
1900 1767 1569
ApaLI G'TGCA_C
Cuts at: 673 4006 673
Size: 3333 1903
ApoI r'AATT_y
Cuts at: 2238 4589 4845 2238
Size: 2351 256 2629
Fragments arranged by size:
2629 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5236
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5236
AvaI C'yCGr_G
Cuts at: 650 1503 2254 650
Size: 853 751 3632
Fragments arranged by size:
3632 853 751
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1344 1377 1344
Size: 33 5203
BamHI G'GATC_C
Cuts at: 2259 2259
Size: 5236
BanI G'GyrC_C
Cuts at: 490 2250 2664 2912 2947 490
Size: 1760 414 248 35 2779
Fragments arranged by size:
2779 1760 414 248 35
BanII G_rGCy'C
Cuts at: 2015 2120 2248 3278 2015
Size: 105 128 1030 3973
Fragments arranged by size:
3973 1030 128 105
BbeI G_GCGC'C
Cuts at: 2668 2916 2668
Size: 248 4988
BbsI GAAGACnn'nnnn_
Cuts at: 532 532
Size: 5236
BbvCI CC'TCA_GC
Cuts at: 557 1375 557
Size: 818 4418
BciVI GTATCCnnnnn_n'
Cuts at: 3132 3895 5236 3132
Size: 763 1341 3132
Fragments arranged by size:
3132 1341 763
BclI T'GATC_A
Cuts at: 2754 2754
Size: 5236
BglI GCCn_nnn'nGGC
Cuts at: 828 1801 828
Size: 973 4263
BglII A'GATC_T
Cuts at: 788 2749 4788 788
Size: 1961 2039 1236
Fragments arranged by size:
2039 1961 1236
BlpI GC'TnA_GC
Cuts at: 1181 2346 1181
Size: 1165 4071
Bme1580I G_kGCm'C
Cuts at: 493 677 2859 2952 4010 493
Size: 184 2182 93 1058 1719
Fragments arranged by size:
2182 1719 1058 184 93
BmrI ACTGGGnnnn_n'
Cuts at: 1722 1882 2446 2527 2862 5008 1722
Size: 160 564 81 335 2146 1950
Fragments arranged by size:
2146 1950 564 335 160 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 177 947 3601 177
Size: 770 2654 1812
Fragments arranged by size:
2654 1812 770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 853
Size: 5236
Bpu10I CC'TnA_GC
Cuts at: 557 611 806 1375 2093 557
Size: 54 195 569 718 3700
Fragments arranged by size:
3700 718 569 195 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1326 1999 2188 2410 3289 3783 4081 1326
Size: 673 189 222 879 494 298 2481
Fragments arranged by size:
2481 879 673 494 298 222 189
BsaI GGTCTCn'nnnn_
Cuts at: 1048 4940 1048
Size: 3892 1344
BsaAI yAC'GTr
Cuts at: 981 2183 2226 2587 981
Size: 1202 43 361 3630
Fragments arranged by size:
3630 1202 361 43
BsaBI GATnn'nnATC
Cuts at: 6 1033 2748 4787 6
Size: 1027 1715 2039 455
Fragments arranged by size:
2039 1715 1027 455
BsaHI Gr'CG_yC
Cuts at: 2665 2913 3617 5113 2665
Size: 248 704 1496 2788
Fragments arranged by size:
2788 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1274 2423 2944 3624 3898 4045 4456 1274
Size: 1149 521 680 274 147 411 2054
Fragments arranged by size:
2054 1149 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1045 1075 2102 2132 1045
Size: 30 1027 30 4149
Fragments arranged by size:
4149 1027 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1823 1826 1823
Size: 3 5233
BseYI C'CCAG_C
Cuts at: 918 1212 1216 1229 1893 2008 2659 3417 3996
Size: 294 4 13 664 115 651 758 579
Cuts at: 3996 4492 918
Size: 496 1662
Fragments arranged by size:
1662 758 664 651 579 496 294 115
13 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 912 1414 2071 883
Size: 29 502 657 4048
Fragments arranged by size:
4048 657 502 29
BsiEI CG_ry'CG
Cuts at: 104 588 2776 2822 4032 104
Size: 484 2188 46 1210 1308
Fragments arranged by size:
2188 1308 1210 484 46
BsiHKAI G_wGCw'C
Cuts at: 677 807 2120 2248 3026 3216 4010 677
Size: 130 1313 128 778 190 794 1903
Fragments arranged by size:
1903 1313 794 778 190 130 128
Bsp1286I G_dGCh'C
Cuts at: 493 677 807 2015 2120 2248 2859 2952 3026
Size: 184 130 1208 105 128 611 93 74
Cuts at: 3026 3216 3278 4010 493
Size: 190 62 732 1719
Fragments arranged by size:
1719 1208 732 611 190 184 130 128
105 93 74 62
BspEI T'CCGG_A
Cuts at: 1274 4456 1274
Size: 3182 2054
BspHI T'CATG_A
Cuts at: 749 4412 749
Size: 3663 1573
BspMI ACCTGCnnnn'nnnn_
Cuts at: 567 1227 2284 2498 2800 3181 567
Size: 660 1057 214 302 381 2622
Fragments arranged by size:
2622 1057 660 381 302 214
BsrBI CCG'CTC
Cuts at: 50 846 1310 2145 3526 3580 5066 5231 50
Size: 796 464 835 1381 54 1486 165 55
Fragments arranged by size:
1486 1381 835 796 464 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1769 3146 1769
Size: 1377 3859
BsrFI r'CCGG_y
Cuts at: 3232 3413 3624 3232
Size: 181 211 4844
Fragments arranged by size:
4844 211 181
BssSI C'ACGA_G
Cuts at: 3505 3865 3505
Size: 360 4876
BstAPI GCAn_nnn'nTGC
Cuts at: 2611 2611
Size: 5236
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5236
BstXI CCAn_nnnn'nTGG
Cuts at: 1735 1735
Size: 5236
BstYI r'GATC_y
Cuts at: 1 788 1601 2069 2259 2726 2749 3084 3330
Size: 787 813 468 190 467 23 335 246
Cuts at: 3330 4344 4430 4442 4788 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 813 787 468 467 449 346 335
246 190 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1043 2152 1043
Size: 1109 4127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 959 992 3184 3365 3528 959
Size: 33 2192 181 163 2667
Fragments arranged by size:
2667 2192 181 163 33
BtsI GCAGTG_nn'
Cuts at: 641 2027 2477 641
Size: 1386 450 3400
Fragments arranged by size:
3400 1386 450
ClaI AT'CG_AT
Cuts at: 1700 1700
Size: 5236
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1595 1630 1595
Size: 35 5201
DraI TTT'AAA
Cuts at: 2233 2233
Size: 5236
DraIII CAC_nnn'GTG
Cuts at: 681 984 1730 681
Size: 303 746 4187
Fragments arranged by size:
4187 746 303
DrdI GACnn_nn'nnGTC
Cuts at: 1270 2185 2940 3800 1270
Size: 915 755 860 2706
Fragments arranged by size:
2706 915 860 755
EaeI y'GGCC_r
Cuts at: 564 683 851 1100 1721 2819 2993 3384 3411
Size: 119 168 249 621 1098 174 391 27
Cuts at: 3411 4758 564
Size: 1347 1042
Fragments arranged by size:
1347 1098 1042 621 391 249 174 168
119 27
EagI C'GGCC_G
Cuts at: 2819 2819
Size: 5236
EarI CTCTTCn'nnn_
Cuts at: 515 3257 3467 515
Size: 2742 210 2284
Fragments arranged by size:
2742 2284 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1877 3442 3754 3900 5046 1877
Size: 1565 312 146 1146 2067
Fragments arranged by size:
2067 1565 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2118 2246 2118
Size: 128 5108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 769 853 1098 1435 1967 3059 3491 4240
Size: 123 84 245 337 532 1092 432 749
Cuts at: 4240 4508 646
Size: 268 1374
Fragments arranged by size:
1374 1092 749 532 432 337 268 245
123 84
EcoNI CCTnn'n_nnAGG
Cuts at: 610 610
Size: 5236
EcoO109I rG'GnC_Cy
Cuts at: 1239 1488 1863 2373 1239
Size: 249 375 510 4102
Fragments arranged by size:
4102 510 375 249
EcoRI G'AATT_C
Cuts at: 2238 2238
Size: 5236
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1371 3253 1371
Size: 1882 3354
FspI TGC'GCA
Cuts at: 3015 3015
Size: 5236
HaeII r_GCGC'y
Cuts at: 2668 2916 3940 4919 2668
Size: 248 1024 979 2985
Fragments arranged by size:
2985 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3076 3106 3108 3138 3076
Size: 30 2 30 5174
Fragments arranged by size:
5174 30 30 2
HincII GTy'rAC
Cuts at: 168 1363 1854 2273 168
Size: 1195 491 419 3131
Fragments arranged by size:
3131 1195 491 419
HindIII A'AGCT_T
Cuts at: 206 206
Size: 5236
KasI G'GCGC_C
Cuts at: 2664 2912 2664
Size: 248 4988
KpnI G_GTAC'C
Cuts at: 2254 2254
Size: 5236
MluI A'CGCG_T
Cuts at: 4669 4669
Size: 5236
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 461 686 1694 3907 4091 461
Size: 225 1008 2213 184 1606
Fragments arranged by size:
2213 1606 1008 225 184
MscI TGG'CCA
Cuts at: 685 1723 2995 685
Size: 1038 1272 2926
Fragments arranged by size:
2926 1272 1038
MslI CAynn'nnrTG
Cuts at: 3223 3350 3223
Size: 127 5109
MspA1I CmG'CkG
Cuts at: 900 1095 1216 1470 2085 2345 2659 3019 4034
Size: 195 121 254 615 260 314 360 1015
Cuts at: 4034 4279 900
Size: 245 1857
Fragments arranged by size:
1857 1015 615 360 314 260 254 245
195 121
NaeI GCC'GGC
Cuts at: 3415 3415
Size: 5236
NarI GG'CG_CC
Cuts at: 2665 2913 2665
Size: 248 4988
NgoMIV G'CCGG_C
Cuts at: 3413 3413
Size: 5236
NruI TCG'CGA
Cuts at: 3597 3597
Size: 5236
NspI r_CATG'y
Cuts at: 2287 2787 3696 2287
Size: 500 909 3827
Fragments arranged by size:
3827 909 500
PasI CC'CwG_GG
Cuts at: 269 269
Size: 5236
PciI A'CATG_T
Cuts at: 3692 3692
Size: 5236
PflMI CCAn_nnn'nTGG
Cuts at: 705 772 984 1730 2139 705
Size: 67 212 746 409 3802
Fragments arranged by size:
3802 746 409 212 67
PmeI GTTT'AAAC
Cuts at: 2233 2233
Size: 5236
PmlI CAC'GTG
Cuts at: 981 981
Size: 5236
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 706 738 2059 2091 4375 4407 706
Size: 32 1321 32 2284 32 1535
Fragments arranged by size:
2284 1535 1321 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1239 1488 1863 1239
Size: 249 375 4612
Fragments arranged by size:
4612 375 249
PstI C_TGCA'G
Cuts at: 578 1300 1500 2281 2966 578
Size: 722 200 781 685 2848
Fragments arranged by size:
2848 781 722 685 200
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5236
PvuII CAG'CTG
Cuts at: 900 1095 1216 2085 2659 3019 900
Size: 195 121 869 574 360 3117
Fragments arranged by size:
3117 869 574 360 195 121
RsrII CG'GwC_CG
Cuts at: 1277 3429 1277
Size: 2152 3084
SacI G_AGCT'C
Cuts at: 2120 2248 2120
Size: 128 5108
SalI G'TCGA_C
Cuts at: 1361 2271 1361
Size: 910 4326
SapI GCTCTTCn'nnn_
Cuts at: 3257 3467 3257
Size: 210 5026
SbfI CC_TGCA'GG
Cuts at: 578 2281 578
Size: 1703 3533
ScaI AGT'ACT
Cuts at: 4753 4753
Size: 5236
SexAI A'CCwGG_T
Cuts at: 698 698
Size: 5236
SfcI C'TryA_G
Cuts at: 33 574 1296 1496 1918 2277 2962 3957 4148
Size: 541 722 200 422 359 685 995 191
Cuts at: 4148 33
Size: 1121
Fragments arranged by size:
1121 995 722 685 541 422 359 200
191
SfoI GGC'GCC
Cuts at: 2666 2914 2666
Size: 248 4988
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5236
SmaI CCC'GGG
Cuts at: 652 1505 2256 652
Size: 853 751 3632
Fragments arranged by size:
3632 853 751
SmlI C'TyrA_G
Cuts at: 1305 2014 2203 2389 3304 3798 4060 1305
Size: 709 189 186 915 494 262 2481
Fragments arranged by size:
2481 915 709 494 262 189 186
SnaBI TAC'GTA
Cuts at: 2226 2226
Size: 5236
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5236
SphI G_CATG'C
Cuts at: 2287 2787 2287
Size: 500 4736
StuI AGG'CCT
Cuts at: 1628 1628
Size: 5236
StyI C'CwwG_G
Cuts at: 2368 2722 2368
Size: 354 4882
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 281 1072 1243 1246 1741 1769 2817 2950 3409
Size: 791 171 3 495 28 1048 133 459
Cuts at: 3409 3620 281
Size: 211 1897
Fragments arranged by size:
1897 1048 791 495 459 211 171 133
28 3
TatI w'GTAC_w
Cuts at: 4751 4751
Size: 5236
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1045 1077 2102 2134 4753 4785 1045
Size: 32 1025 32 2619 32 1496
Fragments arranged by size:
2619 1496 1025 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1318 3031 1318
Size: 1713 3523
XbaI T'CTAG_A
Cuts at: 2265 2265
Size: 5236
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1716 1774 2729 1716
Size: 58 955 4223
Fragments arranged by size:
4223 955 58
XmaI C'CCGG_G
Cuts at: 650 1503 2254 650
Size: 853 751 3632
Fragments arranged by size:
3632 853 751
XmnI GAAnn'nnTTC
Cuts at: 184 208 184
Size: 24 5212
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NruI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I BtgI EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NsiI PacI
PfoI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SrfI SspI SwaI XhoI ZraI