Restriction map for
pF1KSDA0857
(Circular) (Six-base) MAPSORT of: pF1KSDA0857.seq Check: 817 from: 1 to: 5098
pF1KSDA0857 5098 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 485 2134 485
Size: 1649 3449
Acc65I G'GTAC_C
Cuts at: 2112 2112
Size: 5098
AclI AA'CG_TT
Cuts at: 4938 4938
Size: 5098
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 678 1007 1081 1900 2921 3353 4102 4370 678
Size: 329 74 819 1021 432 749 268 1406
Fragments arranged by size:
1406 1021 819 749 432 329 268 74
AflIII A'CryG_T
Cuts at: 254 2448 3554 4531 254
Size: 2194 1106 977 821
Fragments arranged by size:
2194 1106 977 821
AgeI A'CCGG_T
Cuts at: 3486 3486
Size: 5098
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 859 859
Size: 5098
AlwNI CAG_nnn'CTG
Cuts at: 2001 2401 3970 2001
Size: 400 1569 3129
Fragments arranged by size:
3129 1569 400
ApaI G_GGCC'C
Cuts at: 210 369 409 1086 1177 1404 2077 210
Size: 159 40 677 91 227 673 3231
Fragments arranged by size:
3231 677 673 227 159 91 40
ApaLI G'TGCA_C
Cuts at: 3868 3868
Size: 5098
ApoI r'AATT_y
Cuts at: 2100 4451 4707 2100
Size: 2351 256 2491
Fragments arranged by size:
2491 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5098
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5098
AvaI C'yCGr_G
Cuts at: 231 325 1079 1200 2070 2116 231
Size: 94 754 121 870 46 3213
Fragments arranged by size:
3213 870 754 121 94 46
BamHI G'GATC_C
Cuts at: 780 2121 780
Size: 1341 3757
BanI G'GyrC_C
Cuts at: 411 937 1184 2112 2526 2774 2809 411
Size: 526 247 928 414 248 35 2700
Fragments arranged by size:
2700 928 526 414 248 247 35
BanII G_rGCy'C
Cuts at: 210 232 369 409 430 886 1086 1169 1177
Size: 22 137 40 21 456 200 83 8
Cuts at: 1177 1207 1404 1485 1940 2077 2110 3140 210
Size: 30 197 81 455 137 33 1030 2168
Fragments arranged by size:
2168 1030 456 455 200 197 137 137
83 81 40 33 30 22 21 8
BbeI G_GCGC'C
Cuts at: 415 941 2530 2778 415
Size: 526 1589 248 2735
Fragments arranged by size:
2735 1589 526 248
BbsI GAAGACnn'nnnn_
Cuts at: 316 519 316
Size: 203 4895
BbvCI CC'TCA_GC
Cuts at: 903 903
Size: 5098
BciVI GTATCCnnnnn_n'
Cuts at: 2994 3757 5098 2994
Size: 763 1341 2994
Fragments arranged by size:
2994 1341 763
BclI T'GATC_A
Cuts at: 2616 2616
Size: 5098
BglI GCCn_nnn'nGGC
Cuts at: 511 1372 1849 511
Size: 861 477 3760
Fragments arranged by size:
3760 861 477
BglII A'GATC_T
Cuts at: 2611 4650 2611
Size: 2039 3059
BlpI GC'TnA_GC
Cuts at: 1972 2208 1972
Size: 236 4862
Bme1580I G_kGCm'C
Cuts at: 210 369 409 1086 1177 1404 2077 2721 2814
Size: 159 40 677 91 227 673 644 93
Cuts at: 2814 3872 210
Size: 1058 1436
Fragments arranged by size:
1436 1058 677 673 644 227 159 93
91 40
BmgBI CAC'GTC
Cuts at: 186 186
Size: 5098
BmrI ACTGGGnnnn_n'
Cuts at: 858 1685 2308 2389 2724 4870 858
Size: 827 623 81 335 2146 1086
Fragments arranged by size:
2146 1086 827 623 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 360 3463 360
Size: 3103 1995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1406 1746 1813 2023 1406
Size: 340 67 210 4481
Fragments arranged by size:
4481 340 210 67
Bpu10I CC'TnA_GC
Cuts at: 643 903 1349 1780 643
Size: 260 446 431 3961
Fragments arranged by size:
3961 446 431 260
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1680 2272 3151 3645 3943 1680
Size: 592 879 494 298 2835
Fragments arranged by size:
2835 879 592 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 692 1519 2035 4802 692
Size: 827 516 2767 988
Fragments arranged by size:
2767 988 827 516
BsaAI yAC'GTr
Cuts at: 2088 2449 2088
Size: 361 4737
BsaBI GATnn'nnATC
Cuts at: 6 2610 4649 6
Size: 2604 2039 455
Fragments arranged by size:
2604 2039 455
BsaHI Gr'CG_yC
Cuts at: 412 482 938 2527 2775 3479 4975 412
Size: 70 456 1589 248 704 1496 535
Fragments arranged by size:
1589 1496 704 535 456 248 70
BsaWI w'CCGG_w
Cuts at: 2285 2806 3486 3760 3907 4318 2285
Size: 521 680 274 147 411 3065
Fragments arranged by size:
3065 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1177 1207 1177
Size: 30 5068
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 352 937 1064 1067 1177 1270 1351 1405 1408
Size: 585 127 3 110 93 81 54 3
Cuts at: 1408 1443 352
Size: 35 4007
Fragments arranged by size:
4007 585 127 110 93 81 54 35
3 3
BseYI C'CCAG_C
Cuts at: 545 879 2521 3279 3858 4354 545
Size: 334 1642 758 579 496 1289
Fragments arranged by size:
1642 1289 758 579 496 334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 989 2014 553
Size: 436 1025 3637
Fragments arranged by size:
3637 1025 436
BsiEI CG_ry'CG
Cuts at: 104 2019 2638 2684 3894 104
Size: 1915 619 46 1210 1308
Fragments arranged by size:
1915 1308 1210 619 46
BsiHKAI G_wGCw'C
Cuts at: 232 346 430 455 1350 1940 2110 2888 3078
Size: 114 84 25 895 590 170 778 190
Cuts at: 3078 3872 232
Size: 794 1458
Fragments arranged by size:
1458 895 794 778 590 190 170 114
84 25
BsmI GAATG_Cn'
Cuts at: 1823 1823
Size: 5098
Bsp1286I G_dGCh'C
Cuts at: 210 232 346 369 409 430 455 886 1086
Size: 22 114 23 40 21 25 431 200
Cuts at: 1086 1169 1177 1207 1350 1404 1485 1940 2077
Size: 83 8 30 143 54 81 455 137
Cuts at: 2077 2110 2721 2814 2888 3078 3140 3872 210
Size: 33 611 93 74 190 62 732 1436
Fragments arranged by size:
1436 732 611 455 431 200 190 143
137 114 93 83 81 74 62 54
40 33 30 25 23 22 21 8
BspEI T'CCGG_A
Cuts at: 4318 4318
Size: 5098
BspHI T'CATG_A
Cuts at: 4274 4274
Size: 5098
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2146 2360 2662 3043 2146
Size: 214 302 381 4201
Fragments arranged by size:
4201 381 302 214
BsrBI CCG'CTC
Cuts at: 50 238 470 956 1124 1760 1965 3388 3442
Size: 188 232 486 168 636 205 1423 54
Cuts at: 3442 4928 5093 50
Size: 1486 165 55
Fragments arranged by size:
1486 1423 636 486 232 205 188 168
165 55 54
BsrDI GCAATG_nn'
Cuts at: 3008 3008
Size: 5098
BsrFI r'CCGG_y
Cuts at: 150 2018 3094 3275 3486 150
Size: 1868 1076 181 211 1762
Fragments arranged by size:
1868 1762 1076 211 181
BssSI C'ACGA_G
Cuts at: 3367 3727 3367
Size: 360 4738
BstAPI GCAn_nnn'nTGC
Cuts at: 2473 2473
Size: 5098
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5098
BstXI CCAn_nnnn'nTGG
Cuts at: 132 934 132
Size: 802 4296
BstYI r'GATC_y
Cuts at: 1 780 2063 2121 2588 2611 2946 3192 4206
Size: 779 1283 58 467 23 335 246 1014
Cuts at: 4206 4292 4304 4650 1
Size: 86 12 346 449
Fragments arranged by size:
1283 1014 779 467 449 346 335 246
86 58 23 12
BtgI C'CryG_G
Cuts at: 125 210 506 125
Size: 85 296 4717
Fragments arranged by size:
4717 296 85
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1061 3046 3227 3390 1061
Size: 1985 181 163 2769
Fragments arranged by size:
2769 1985 181 163
BtsI GCAGTG_nn'
Cuts at: 562 1328 1791 2339 562
Size: 766 463 548 3321
Fragments arranged by size:
3321 766 548 463
DraI TTT'AAA
Cuts at: 2095 2095
Size: 5098
DraIII CAC_nnn'GTG
Cuts at: 557 1704 557
Size: 1147 3951
DrdI GACnn_nn'nnGTC
Cuts at: 2802 3662 2802
Size: 860 4238
EaeI y'GGCC_r
Cuts at: 1854 1907 2681 2855 3246 3273 4620 1854
Size: 53 774 174 391 27 1347 2332
Fragments arranged by size:
2332 1347 774 391 174 53 27
EagI C'GGCC_G
Cuts at: 2681 2681
Size: 5098
EarI CTCTTCn'nnn_
Cuts at: 1131 1245 1522 3119 3329 1131
Size: 114 277 1597 210 2900
Fragments arranged by size:
2900 1597 277 210 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 420 3304 3616 3762 4908 420
Size: 2884 312 146 1146 610
Fragments arranged by size:
2884 1146 610 312 146
EcoICRI GAG'CTC
Cuts at: 230 428 1938 2108 230
Size: 198 1510 170 3220
Fragments arranged by size:
3220 1510 198 170
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 678 1007 1081 1406 1746 1813 1900 2023 2921
Size: 329 74 325 340 67 87 123 898
Cuts at: 2921 3353 4102 4370 678
Size: 432 749 268 1406
Fragments arranged by size:
1406 898 749 432 340 329 325 268
123 87 74 67
EcoNI CCTnn'n_nnAGG
Cuts at: 499 499
Size: 5098
EcoO109I rG'GnC_Cy
Cuts at: 160 365 366 931 1037 1083 1173 1174 1261
Size: 205 1 565 106 46 90 1 87
Cuts at: 1261 1400 1588 1601 1674 1842 2074 2235 160
Size: 139 188 13 73 168 232 161 3023
Fragments arranged by size:
3023 565 232 205 188 168 161 139
106 90 87 73 46 13 1 1
EcoRI G'AATT_C
Cuts at: 2100 2100
Size: 5098
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3115 3115
Size: 5098
FspI TGC'GCA
Cuts at: 847 2877 847
Size: 2030 3068
HaeII r_GCGC'y
Cuts at: 415 517 650 773 941 2530 2778 3802 4781
Size: 102 133 123 168 1589 248 1024 979
Cuts at: 4781 415
Size: 732
Fragments arranged by size:
1589 1024 979 732 248 168 133 123
102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 727 759 1102 1134 2938 2968 2970 3000 727
Size: 32 343 32 1804 30 2 30 2825
Fragments arranged by size:
2825 1804 343 32 32 30 30 2
HincII GTy'rAC
Cuts at: 486 2135 486
Size: 1649 3449
KasI G'GCGC_C
Cuts at: 411 937 2526 2774 411
Size: 526 1589 248 2735
Fragments arranged by size:
2735 1589 526 248
KpnI G_GTAC'C
Cuts at: 2116 2116
Size: 5098
MluI A'CGCG_T
Cuts at: 254 4531 254
Size: 4277 821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 893 1525 3769 3953 893
Size: 632 2244 184 2038
Fragments arranged by size:
2244 2038 632 184
MscI TGG'CCA
Cuts at: 1856 2857 1856
Size: 1001 4097
MslI CAynn'nnrTG
Cuts at: 460 1109 3085 3212 460
Size: 649 1976 127 2346
Fragments arranged by size:
2346 1976 649 127
MspA1I CmG'CkG
Cuts at: 170 212 911 974 1891 1959 1980 2207 2521
Size: 42 699 63 917 68 21 227 314
Cuts at: 2521 2881 3896 4141 170
Size: 360 1015 245 1127
Fragments arranged by size:
1127 1015 917 699 360 314 245 227
68 63 42 21
NaeI GCC'GGC
Cuts at: 152 3277 152
Size: 3125 1973
NarI GG'CG_CC
Cuts at: 412 938 2527 2775 412
Size: 526 1589 248 2735
Fragments arranged by size:
2735 1589 526 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 5098
NgoMIV G'CCGG_C
Cuts at: 150 3275 150
Size: 3125 1973
NruI TCG'CGA
Cuts at: 3459 3459
Size: 5098
NspI r_CATG'y
Cuts at: 1993 2149 2649 3558 1993
Size: 156 500 909 3533
Fragments arranged by size:
3533 909 500 156
PasI CC'CwG_GG
Cuts at: 416 1170 1369 416
Size: 754 199 4145
Fragments arranged by size:
4145 754 199
PciI A'CATG_T
Cuts at: 3554 3554
Size: 5098
PflMI CCAn_nnn'nTGG
Cuts at: 1219 1863 1219
Size: 644 4454
PmeI GTTT'AAAC
Cuts at: 2095 2095
Size: 5098
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4237 4269 4237
Size: 32 5066
PpuMI rG'GwC_Cy
Cuts at: 931 1037 1588 1674 931
Size: 106 551 86 4355
Fragments arranged by size:
4355 551 106 86
PspOMI G'GGCC_C
Cuts at: 206 365 405 1082 1173 1400 2073 206
Size: 159 40 677 91 227 673 3231
Fragments arranged by size:
3231 677 673 227 159 91 40
PstI C_TGCA'G
Cuts at: 1958 2063 2143 2828 1958
Size: 105 80 685 4228
Fragments arranged by size:
4228 685 105 80
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5098
PvuII CAG'CTG
Cuts at: 974 1891 2521 2881 974
Size: 917 630 360 3191
Fragments arranged by size:
3191 917 630 360
RsrII CG'GwC_CG
Cuts at: 3291 3291
Size: 5098
SacI G_AGCT'C
Cuts at: 232 430 1940 2110 232
Size: 198 1510 170 3220
Fragments arranged by size:
3220 1510 198 170
SacII CC_GC'GG
Cuts at: 213 213
Size: 5098
SalI G'TCGA_C
Cuts at: 484 2133 484
Size: 1649 3449
SanDI GG'GwC_CC
Cuts at: 1588 1588
Size: 5098
SapI GCTCTTCn'nnn_
Cuts at: 1131 3119 3329 1131
Size: 1988 210 2900
Fragments arranged by size:
2900 1988 210
SbfI CC_TGCA'GG
Cuts at: 2143 2143
Size: 5098
ScaI AGT'ACT
Cuts at: 1915 4615 1915
Size: 2700 2398
SfcI C'TryA_G
Cuts at: 33 1041 1954 2059 2139 2824 3819 4010 33
Size: 1008 913 105 80 685 995 191 1121
Fragments arranged by size:
1121 1008 995 913 685 191 105 80
SfiI GGCCn_nnn'nGGCC
Cuts at: 1372 1372
Size: 5098
SfoI GGC'GCC
Cuts at: 413 939 2528 2776 413
Size: 526 1589 248 2735
Fragments arranged by size:
2735 1589 526 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5098
SmaI CCC'GGG
Cuts at: 2072 2118 2072
Size: 46 5052
SmlI C'TyrA_G
Cuts at: 1659 2251 3166 3660 3922 1659
Size: 592 915 494 262 2835
Fragments arranged by size:
2835 915 592 494 262
SnaBI TAC'GTA
Cuts at: 2088 2088
Size: 5098
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5098
SphI G_CATG'C
Cuts at: 2149 2649 2149
Size: 500 4598
StuI AGG'CCT
Cuts at: 1538 1538
Size: 5098
StyI C'CwwG_G
Cuts at: 125 682 1533 1670 2230 2584 125
Size: 557 851 137 560 354 2639
Fragments arranged by size:
2639 851 560 557 354 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1042 1455 2064 2679 2812 3271 3482 1042
Size: 413 609 615 133 459 211 2658
Fragments arranged by size:
2658 615 609 459 413 211 133
TatI w'GTAC_w
Cuts at: 287 1913 4613 287
Size: 1626 2700 772
Fragments arranged by size:
2700 1626 772
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1177 1209 4615 4647 1177
Size: 32 3406 32 1628
Fragments arranged by size:
3406 1628 32 32
Tth111I GACn'n_nGTC
Cuts at: 523 864 2893 523
Size: 341 2029 2728
Fragments arranged by size:
2728 2029 341
XbaI T'CTAG_A
Cuts at: 2127 2127
Size: 5098
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 513 876 915 1443 2591 513
Size: 363 39 528 1148 3020
Fragments arranged by size:
3020 1148 528 363 39
XmaI C'CCGG_G
Cuts at: 2070 2116 2070
Size: 46 5052
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PasI PciI PflMI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BcgI BfrBI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NsiI
PacI PfoI PmlI PshAI PsiI PspXI PsrI SexAI
SgrAI SrfI SspI SwaI XhoI XmnI ZraI