Restriction map for
pF1KSDA0846
(Circular) (Six-base) MAPSORT of: pF1KSDA0846.seq Check: 240 from: 1 to: 5206
pF1KSDA0846 5206 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 2123 2242 2123
Size: 119 5087
Acc65I G'GTAC_C
Cuts at: 1320 2220 1320
Size: 900 4306
AclI AA'CG_TT
Cuts at: 5046 5046
Size: 5206
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 808 902 1253 3029 3461 4210 4478 394
Size: 414 94 351 1776 432 749 268 1122
Fragments arranged by size:
1776 1122 749 432 414 351 268 94
AflIII A'CryG_T
Cuts at: 465 2556 3662 4639 465
Size: 2091 1106 977 1032
Fragments arranged by size:
2091 1106 1032 977
AgeI A'CCGG_T
Cuts at: 3594 3594
Size: 5206
AhdI GACnn_n'nnGTC
Cuts at: 2007 2007
Size: 5206
AlwNI CAG_nnn'CTG
Cuts at: 1990 2509 4078 1990
Size: 519 1569 3118
Fragments arranged by size:
3118 1569 519
ApaLI G'TGCA_C
Cuts at: 174 3976 174
Size: 3802 1404
ApoI r'AATT_y
Cuts at: 331 427 834 1275 2208 4559 4815 331
Size: 96 407 441 933 2351 256 722
Fragments arranged by size:
2351 933 722 441 407 256 96
AseI AT'TA_AT
Cuts at: 20 20
Size: 5206
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5206
AvaI C'yCGr_G
Cuts at: 1766 2004 2224 1766
Size: 238 220 4748
Fragments arranged by size:
4748 238 220
BamHI G'GATC_C
Cuts at: 2229 2229
Size: 5206
BanI G'GyrC_C
Cuts at: 1320 1770 2220 2634 2882 2917 1320
Size: 450 450 414 248 35 3609
Fragments arranged by size:
3609 450 450 414 248 35
BanII G_rGCy'C
Cuts at: 584 2218 3248 584
Size: 1634 1030 2542
Fragments arranged by size:
2542 1634 1030
BbeI G_GCGC'C
Cuts at: 1774 2638 2886 1774
Size: 864 248 4094
Fragments arranged by size:
4094 864 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1636 1670 1636
Size: 34 5172
BciVI GTATCCnnnnn_n'
Cuts at: 549 1681 3102 3865 5206 549
Size: 1132 1421 763 1341 549
Fragments arranged by size:
1421 1341 1132 763 549
BclI T'GATC_A
Cuts at: 1193 1419 2724 1193
Size: 226 1305 3675
Fragments arranged by size:
3675 1305 226
BglII A'GATC_T
Cuts at: 344 1922 2719 4758 344
Size: 1578 797 2039 792
Fragments arranged by size:
2039 1578 797 792
BlpI GC'TnA_GC
Cuts at: 596 2316 596
Size: 1720 3486
Bme1580I G_kGCm'C
Cuts at: 178 2134 2829 2922 3980 178
Size: 1956 695 93 1058 1404
Fragments arranged by size:
1956 1404 1058 695 93
BmgBI CAC'GTC
Cuts at: 468 468
Size: 5206
BmrI ACTGGGnnnn_n'
Cuts at: 2416 2497 2832 4978 2416
Size: 81 335 2146 2644
Fragments arranged by size:
2644 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 975 3571 975
Size: 2596 2610
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 598 634 700 1285 1348 1874 335
Size: 263 36 66 585 63 526 3667
Fragments arranged by size:
3667 585 526 263 66 63 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1615 1800 2380 3259 3753 4051 1615
Size: 185 580 879 494 298 2770
Fragments arranged by size:
2770 879 580 494 298 185
BsaI GGTCTCn'nnnn_
Cuts at: 892 963 2161 4910 892
Size: 71 1198 2749 1188
Fragments arranged by size:
2749 1198 1188 71
BsaAI yAC'GTr
Cuts at: 2196 2557 2196
Size: 361 4845
BsaBI GATnn'nnATC
Cuts at: 6 2718 4757 6
Size: 2712 2039 455
Fragments arranged by size:
2712 2039 455
BsaHI Gr'CG_yC
Cuts at: 1303 1771 2635 2883 3587 5083 1303
Size: 468 864 248 704 1496 1426
Fragments arranged by size:
1496 1426 864 704 468 248
BsaWI w'CCGG_w
Cuts at: 2393 2914 3594 3868 4015 4426 2393
Size: 521 680 274 147 411 3173
Fragments arranged by size:
3173 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1108 1138 1108
Size: 30 5176
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 951 1102 951
Size: 151 5055
BseYI C'CCAG_C
Cuts at: 770 1824 1994 2629 3387 3966 4462 770
Size: 1054 170 635 758 579 496 1514
Fragments arranged by size:
1514 1054 758 635 579 496 170
BsiEI CG_ry'CG
Cuts at: 104 2746 2792 4002 104
Size: 2642 46 1210 1308
Fragments arranged by size:
2642 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 178 602 2218 2996 3186 3980 178
Size: 424 1616 778 190 794 1404
Fragments arranged by size:
1616 1404 794 778 424 190
BsmI GAATG_Cn'
Cuts at: 491 1164 491
Size: 673 4533
Bsp1286I G_dGCh'C
Cuts at: 178 584 602 2134 2218 2829 2922 2996 3186
Size: 406 18 1532 84 611 93 74 190
Cuts at: 3186 3248 3980 178
Size: 62 732 1404
Fragments arranged by size:
1532 1404 732 611 406 190 93 84
74 62 18
BspEI T'CCGG_A
Cuts at: 4426 4426
Size: 5206
BspHI T'CATG_A
Cuts at: 356 4382 356
Size: 4026 1180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1208 2254 2468 2770 3151 1208
Size: 1046 214 302 381 3263
Fragments arranged by size:
3263 1046 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3496 3550 5036 5201 50
Size: 3446 54 1486 165 55
Fragments arranged by size:
3446 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 333 3116 333
Size: 2783 2423
BsrFI r'CCGG_y
Cuts at: 3202 3383 3594 3202
Size: 181 211 4814
Fragments arranged by size:
4814 211 181
BssSI C'ACGA_G
Cuts at: 3475 3835 3475
Size: 360 4846
BstAPI GCAn_nnn'nTGC
Cuts at: 977 2581 977
Size: 1604 3602
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5206
BstXI CCAn_nnnn'nTGG
Cuts at: 276 591 1899 276
Size: 315 1308 3583
Fragments arranged by size:
3583 1308 315
BstYI r'GATC_y
Cuts at: 1 344 399 635 1922 2229 2696 2719 3054
Size: 343 55 236 1287 307 467 23 335
Cuts at: 3054 3300 4314 4400 4412 4758 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1287 1014 467 449 346 343 335 307
246 236 86 55 23 12
BtgI C'CryG_G
Cuts at: 125 669 1364 125
Size: 544 695 3967
Fragments arranged by size:
3967 695 544
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1022 3154 3335 3498 1022
Size: 2132 181 163 2730
Fragments arranged by size:
2730 2132 181 163
BtsI GCAGTG_nn'
Cuts at: 860 1791 1798 1859 2447 860
Size: 931 7 61 588 3619
Fragments arranged by size:
3619 931 588 61 7
ClaI AT'CG_AT
Cuts at: 706 706
Size: 5206
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1686 1721 1686
Size: 35 5171
DraI TTT'AAA
Cuts at: 2203 2203
Size: 5206
DrdI GACnn_nn'nnGTC
Cuts at: 1366 2910 3770 1366
Size: 1544 860 2802
Fragments arranged by size:
2802 1544 860
EaeI y'GGCC_r
Cuts at: 2789 2963 3354 3381 4728 2789
Size: 174 391 27 1347 3267
Fragments arranged by size:
3267 1347 391 174 27
EagI C'GGCC_G
Cuts at: 2789 2789
Size: 5206
EarI CTCTTCn'nnn_
Cuts at: 510 1917 2159 3227 3437 510
Size: 1407 242 1068 210 2279
Fragments arranged by size:
2279 1407 1068 242 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3412 3724 3870 5016 3412
Size: 312 146 1146 3602
Fragments arranged by size:
3602 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2216 2216
Size: 5206
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 394 598 634 700 808 902 1253 1285
Size: 59 204 36 66 108 94 351 32
Cuts at: 1285 1348 1874 3029 3461 4210 4478 335
Size: 63 526 1155 432 749 268 1063
Fragments arranged by size:
1155 1063 749 526 432 351 268 204
108 94 66 63 59 36 32
EcoO109I rG'GnC_Cy
Cuts at: 2009 2343 2009
Size: 334 4872
EcoRI G'AATT_C
Cuts at: 2208 2208
Size: 5206
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1656 3223 1656
Size: 1567 3639
FspI TGC'GCA
Cuts at: 2985 2985
Size: 5206
HaeII r_GCGC'y
Cuts at: 1774 2638 2886 3910 4889 1774
Size: 864 248 1024 979 2091
Fragments arranged by size:
2091 1024 979 864 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 329 361 607 639 891 923 1611 1643 1773
Size: 32 246 32 252 32 688 32 130
Cuts at: 1773 1805 1993 2025 3046 3076 3078 3108 329
Size: 32 188 32 1021 30 2 30 2427
Fragments arranged by size:
2427 1021 688 252 246 188 130 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 448 2243 448
Size: 1795 3411
KasI G'GCGC_C
Cuts at: 1770 2634 2882 1770
Size: 864 248 4094
Fragments arranged by size:
4094 864 248
KpnI G_GTAC'C
Cuts at: 1324 2224 1324
Size: 900 4306
MfeI C'AATT_G
Cuts at: 1713 1713
Size: 5206
MluI A'CGCG_T
Cuts at: 4639 4639
Size: 5206
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 715 989 3877 4061 715
Size: 274 2888 184 1860
Fragments arranged by size:
2888 1860 274 184
MscI TGG'CCA
Cuts at: 2965 2965
Size: 5206
MslI CAynn'nnrTG
Cuts at: 187 847 1427 2108 3193 3320 187
Size: 660 580 681 1085 127 2073
Fragments arranged by size:
2073 1085 681 660 580 127
MspA1I CmG'CkG
Cuts at: 2315 2629 2989 4004 4249 2315
Size: 314 360 1015 245 3272
Fragments arranged by size:
3272 1015 360 314 245
NaeI GCC'GGC
Cuts at: 3385 3385
Size: 5206
NarI GG'CG_CC
Cuts at: 1771 2635 2883 1771
Size: 864 248 4094
Fragments arranged by size:
4094 864 248
NcoI C'CATG_G
Cuts at: 125 669 125
Size: 544 4662
NgoMIV G'CCGG_C
Cuts at: 3383 3383
Size: 5206
NruI TCG'CGA
Cuts at: 3567 3567
Size: 5206
NspI r_CATG'y
Cuts at: 300 2107 2257 2757 3666 300
Size: 1807 150 500 909 1840
Fragments arranged by size:
1840 1807 909 500 150
PasI CC'CwG_GG
Cuts at: 2096 2096
Size: 5206
PciI A'CATG_T
Cuts at: 3662 3662
Size: 5206
PflMI CCAn_nnn'nTGG
Cuts at: 747 747
Size: 5206
PmeI GTTT'AAAC
Cuts at: 2203 2203
Size: 5206
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4345 4377 4345
Size: 32 5174
PpuMI rG'GwC_Cy
Cuts at: 2009 2009
Size: 5206
PstI C_TGCA'G
Cuts at: 387 1157 1756 2251 2936 387
Size: 770 599 495 685 2657
Fragments arranged by size:
2657 770 685 599 495
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5206
PvuII CAG'CTG
Cuts at: 2629 2989 2629
Size: 360 4846
RsrII CG'GwC_CG
Cuts at: 3399 3399
Size: 5206
SacI G_AGCT'C
Cuts at: 2218 2218
Size: 5206
SalI G'TCGA_C
Cuts at: 2241 2241
Size: 5206
SanDI GG'GwC_CC
Cuts at: 2009 2009
Size: 5206
SapI GCTCTTCn'nnn_
Cuts at: 1917 3227 3437 1917
Size: 1310 210 3686
Fragments arranged by size:
3686 1310 210
SbfI CC_TGCA'GG
Cuts at: 1756 2251 1756
Size: 495 4711
ScaI AGT'ACT
Cuts at: 4723 4723
Size: 5206
SfcI C'TryA_G
Cuts at: 33 383 1153 1752 1936 2247 2932 3927 4118
Size: 350 770 599 184 311 685 995 191
Cuts at: 4118 33
Size: 1121
Fragments arranged by size:
1121 995 770 685 599 350 311 191
184
SfoI GGC'GCC
Cuts at: 1772 2636 2884 1772
Size: 864 248 4094
Fragments arranged by size:
4094 864 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5206
SmaI CCC'GGG
Cuts at: 1768 2226 1768
Size: 458 4748
SmlI C'TyrA_G
Cuts at: 1630 1779 2359 3274 3768 4030 1630
Size: 149 580 915 494 262 2806
Fragments arranged by size:
2806 915 580 494 262 149
SnaBI TAC'GTA
Cuts at: 2196 2196
Size: 5206
SpeI A'CTAG_T
Cuts at: 67 1143 67
Size: 1076 4130
SphI G_CATG'C
Cuts at: 2107 2257 2757 2107
Size: 150 500 4556
Fragments arranged by size:
4556 500 150
SrfI GCCC'GGGC
Cuts at: 1768 1768
Size: 5206
StuI AGG'CCT
Cuts at: 866 994 866
Size: 128 5078
StyI C'CwwG_G
Cuts at: 125 141 669 695 1029 2338 2692 125
Size: 16 528 26 334 1309 354 2639
Fragments arranged by size:
2639 1309 528 354 334 26 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1978 2787 2920 3379 3590 1978
Size: 809 133 459 211 3594
Fragments arranged by size:
3594 809 459 211 133
TatI w'GTAC_w
Cuts at: 1035 4721 1035
Size: 3686 1520
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1108 1140 4723 4755 1108
Size: 32 3583 32 1559
Fragments arranged by size:
3583 1559 32 32
Tth111I GACn'n_nGTC
Cuts at: 3001 3001
Size: 5206
XbaI T'CTAG_A
Cuts at: 2235 2235
Size: 5206
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1314 2699 1314
Size: 1385 3821
XmaI C'CCGG_G
Cuts at: 1766 2224 1766
Size: 458 4748
XmnI GAAnn'nnTTC
Cuts at: 287 287
Size: 5206
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PasI PciI PflMI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmtI
Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NsiI PacI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SspI SwaI XhoI ZraI