Restriction map for
pF1KSDA0844
(Circular) (Six-base) MAPSORT of: pF1KSDA0844.seq Check: 6588 from: 1 to: 4360
pF1KSDA0844 4360 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 1396 1396
Size: 4360
Acc65I G'GTAC_C
Cuts at: 1374 1374
Size: 4360
AclI AA'CG_TT
Cuts at: 4200 4200
Size: 4360
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2183 2615 3364 3632 2183
Size: 432 749 268 2911
Fragments arranged by size:
2911 749 432 268
AflIII A'CryG_T
Cuts at: 603 1710 2816 3793 603
Size: 1107 1106 977 1170
Fragments arranged by size:
1170 1107 1106 977
AgeI A'CCGG_T
Cuts at: 2748 2748
Size: 4360
AlwNI CAG_nnn'CTG
Cuts at: 1663 3232 1663
Size: 1569 2791
ApaI G_GGCC'C
Cuts at: 261 261
Size: 4360
ApaLI G'TGCA_C
Cuts at: 3130 3130
Size: 4360
ApoI r'AATT_y
Cuts at: 616 1362 3713 3969 616
Size: 746 2351 256 1007
Fragments arranged by size:
2351 1007 746 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4360
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4360
AvaI C'yCGr_G
Cuts at: 210 1378 210
Size: 1168 3192
BamHI G'GATC_C
Cuts at: 1383 1383
Size: 4360
BanI G'GyrC_C
Cuts at: 1097 1374 1788 2036 2071 1097
Size: 277 414 248 35 3386
Fragments arranged by size:
3386 414 277 248 35
BanII G_rGCy'C
Cuts at: 261 1271 1372 2402 261
Size: 1010 101 1030 2219
Fragments arranged by size:
2219 1030 1010 101
BbeI G_GCGC'C
Cuts at: 1792 2040 1792
Size: 248 4112
BciVI GTATCCnnnnn_n'
Cuts at: 2256 3019 4360 2256
Size: 763 1341 2256
Fragments arranged by size:
2256 1341 763
BclI T'GATC_A
Cuts at: 1878 1878
Size: 4360
BglI GCCn_nnn'nGGC
Cuts at: 1289 1289
Size: 4360
BglII A'GATC_T
Cuts at: 1873 3912 1873
Size: 2039 2321
BlpI GC'TnA_GC
Cuts at: 1470 1470
Size: 4360
Bme1580I G_kGCm'C
Cuts at: 261 1046 1100 1983 2076 3134 261
Size: 785 54 883 93 1058 1487
Fragments arranged by size:
1487 1058 883 785 93 54
BmgBI CAC'GTC
Cuts at: 795 795
Size: 4360
BmrI ACTGGGnnnn_n'
Cuts at: 1570 1651 1986 4132 1570
Size: 81 335 2146 1798
Fragments arranged by size:
2146 1798 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2725 2725
Size: 4360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 922 286
Size: 636 3724
Bpu10I CC'TnA_GC
Cuts at: 1087 1087
Size: 4360
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 273 1012 1114 1218 1534 2413 2907 3205 273
Size: 739 102 104 316 879 494 298 1428
Fragments arranged by size:
1428 879 739 494 316 298 104 102
BsaI GGTCTCn'nnnn_
Cuts at: 215 1232 4064 215
Size: 1017 2832 511
Fragments arranged by size:
2832 1017 511
BsaAI yAC'GTr
Cuts at: 1047 1350 1711 1047
Size: 303 361 3696
Fragments arranged by size:
3696 361 303
BsaBI GATnn'nnATC
Cuts at: 6 1872 3911 6
Size: 1866 2039 455
Fragments arranged by size:
2039 1866 455
BsaHI Gr'CG_yC
Cuts at: 1789 2037 2741 4237 1789
Size: 248 704 1496 1912
Fragments arranged by size:
1912 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1547 2068 2748 3022 3169 3580 1547
Size: 521 680 274 147 411 2327
Fragments arranged by size:
2327 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 859 862 1053 859
Size: 3 191 4166
Fragments arranged by size:
4166 191 3
BseYI C'CCAG_C
Cuts at: 1783 2541 3120 3616 1783
Size: 758 579 496 2527
Fragments arranged by size:
2527 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 751 523
Size: 228 4132
BsiEI CG_ry'CG
Cuts at: 104 556 1900 1946 3156 104
Size: 452 1344 46 1210 1308
Fragments arranged by size:
1344 1308 1210 452 46
BsiHKAI G_wGCw'C
Cuts at: 1271 1372 2150 2340 3134 1271
Size: 101 778 190 794 2497
Fragments arranged by size:
2497 794 778 190 101
BsmI GAATG_Cn'
Cuts at: 1104 1104
Size: 4360
BsmBI CGTCTCn'nnnn_
Cuts at: 756 756
Size: 4360
Bsp1286I G_dGCh'C
Cuts at: 261 1046 1100 1271 1372 1983 2076 2150 2340
Size: 785 54 171 101 611 93 74 190
Cuts at: 2340 2402 3134 261
Size: 62 732 1487
Fragments arranged by size:
1487 785 732 611 190 171 101 93
74 62 54
BspEI T'CCGG_A
Cuts at: 3580 3580
Size: 4360
BspHI T'CATG_A
Cuts at: 3536 3536
Size: 4360
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1408 1622 1924 2305 1408
Size: 214 302 381 3463
Fragments arranged by size:
3463 381 302 214
BsrBI CCG'CTC
Cuts at: 50 771 2650 2704 4190 4355 50
Size: 721 1879 54 1486 165 55
Fragments arranged by size:
1879 1486 721 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2270 2270
Size: 4360
BsrFI r'CCGG_y
Cuts at: 2356 2537 2748 2356
Size: 181 211 3968
Fragments arranged by size:
3968 211 181
BsrGI T'GTAC_A
Cuts at: 428 428
Size: 4360
BssSI C'ACGA_G
Cuts at: 2629 2989 2629
Size: 360 4000
BstAPI GCAn_nnn'nTGC
Cuts at: 1735 1735
Size: 4360
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4360
BstXI CCAn_nnnn'nTGG
Cuts at: 684 684
Size: 4360
BstYI r'GATC_y
Cuts at: 1 1383 1850 1873 2208 2454 3468 3554 3566
Size: 1382 467 23 335 246 1014 86 12
Cuts at: 3566 3912 1
Size: 346 449
Fragments arranged by size:
1382 1014 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 518 800 518
Size: 282 4078
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2308 2489 2652 2308
Size: 181 163 4016
Fragments arranged by size:
4016 181 163
BtsI GCAGTG_nn'
Cuts at: 393 512 1601 393
Size: 119 1089 3152
Fragments arranged by size:
3152 1089 119
DraI TTT'AAA
Cuts at: 1357 1357
Size: 4360
DrdI GACnn_nn'nnGTC
Cuts at: 2064 2924 2064
Size: 860 3500
EaeI y'GGCC_r
Cuts at: 797 1281 1943 2117 2508 2535 3882 797
Size: 484 662 174 391 27 1347 1275
Fragments arranged by size:
1347 1275 662 484 391 174 27
EagI C'GGCC_G
Cuts at: 1943 1943
Size: 4360
EarI CTCTTCn'nnn_
Cuts at: 652 2381 2591 652
Size: 1729 210 2421
Fragments arranged by size:
2421 1729 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 994 2566 2878 3024 4170 994
Size: 1572 312 146 1146 1184
Fragments arranged by size:
1572 1184 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1269 1370 1269
Size: 101 4259
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 922 2183 2615 3364 3632 286
Size: 636 1261 432 749 268 1014
Fragments arranged by size:
1261 1014 749 636 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1068 1168 1068
Size: 100 4260
EcoO109I rG'GnC_Cy
Cuts at: 257 559 1497 257
Size: 302 938 3120
Fragments arranged by size:
3120 938 302
EcoRI G'AATT_C
Cuts at: 1362 1362
Size: 4360
EcoRV GAT'ATC
Cuts at: 157 157
Size: 4360
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 4360
FspI TGC'GCA
Cuts at: 2139 2139
Size: 4360
HaeII r_GCGC'y
Cuts at: 1792 2040 3064 4043 1792
Size: 248 1024 979 2109
Fragments arranged by size:
2109 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 246 278 1048 1080 1117 1149 2200 2230 2232
Size: 32 770 32 37 32 1051 30 2
Cuts at: 2232 2262 246
Size: 30 2344
Fragments arranged by size:
2344 1051 770 37 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 423 1397 423
Size: 974 3386
HindIII A'AGCT_T
Cuts at: 240 587 240
Size: 347 4013
HpaI GTT'AAC
Cuts at: 423 423
Size: 4360
KasI G'GCGC_C
Cuts at: 1788 2036 1788
Size: 248 4112
KpnI G_GTAC'C
Cuts at: 1378 1378
Size: 4360
MluI A'CGCG_T
Cuts at: 3793 3793
Size: 4360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 689 3031 3215 689
Size: 2342 184 1834
Fragments arranged by size:
2342 1834 184
MscI TGG'CCA
Cuts at: 2119 2119
Size: 4360
MslI CAynn'nnrTG
Cuts at: 517 2347 2474 517
Size: 1830 127 2403
Fragments arranged by size:
2403 1830 127
MspA1I CmG'CkG
Cuts at: 735 907 966 1040 1469 1783 2143 3158 3403
Size: 172 59 74 429 314 360 1015 245
Cuts at: 3403 735
Size: 1692
Fragments arranged by size:
1692 1015 429 360 314 245 172 74
59
NaeI GCC'GGC
Cuts at: 2539 2539
Size: 4360
NarI GG'CG_CC
Cuts at: 1789 2037 1789
Size: 248 4112
NcoI C'CATG_G
Cuts at: 518 518
Size: 4360
NgoMIV G'CCGG_C
Cuts at: 2537 2537
Size: 4360
NruI TCG'CGA
Cuts at: 2721 2721
Size: 4360
NspI r_CATG'y
Cuts at: 607 1197 1411 1911 2820 607
Size: 590 214 500 909 2147
Fragments arranged by size:
2147 909 590 500 214
PciI A'CATG_T
Cuts at: 603 2816 603
Size: 2213 2147
PflMI CCAn_nnn'nTGG
Cuts at: 894 894
Size: 4360
PfoI T'CCnGG_A
Cuts at: 561 893 938 561
Size: 332 45 3983
Fragments arranged by size:
3983 332 45
PmeI GTTT'AAAC
Cuts at: 1357 1357
Size: 4360
PmlI CAC'GTG
Cuts at: 1047 1047
Size: 4360
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3499 3531 3499
Size: 32 4328
PpuMI rG'GwC_Cy
Cuts at: 559 559
Size: 4360
PspOMI G'GGCC_C
Cuts at: 257 257
Size: 4360
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1330 1362 1330
Size: 32 4328
PstI C_TGCA'G
Cuts at: 1249 1405 2090 1249
Size: 156 685 3519
Fragments arranged by size:
3519 685 156
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4360
PvuII CAG'CTG
Cuts at: 735 907 1783 2143 735
Size: 172 876 360 2952
Fragments arranged by size:
2952 876 360 172
RsrII CG'GwC_CG
Cuts at: 2553 2553
Size: 4360
SacI G_AGCT'C
Cuts at: 1271 1372 1271
Size: 101 4259
SalI G'TCGA_C
Cuts at: 1395 1395
Size: 4360
SapI GCTCTTCn'nnn_
Cuts at: 2381 2591 2381
Size: 210 4150
SbfI CC_TGCA'GG
Cuts at: 1405 1405
Size: 4360
ScaI AGT'ACT
Cuts at: 997 3877 997
Size: 2880 1480
SfcI C'TryA_G
Cuts at: 33 1245 1401 2086 3081 3272 33
Size: 1212 156 685 995 191 1121
Fragments arranged by size:
1212 1121 995 685 191 156
SfoI GGC'GCC
Cuts at: 1790 2038 1790
Size: 248 4112
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4360
SmaI CCC'GGG
Cuts at: 1380 1380
Size: 4360
SmlI C'TyrA_G
Cuts at: 252 991 1129 1233 1513 2428 2922 3184 252
Size: 739 138 104 280 915 494 262 1428
Fragments arranged by size:
1428 915 739 494 280 262 138 104
SnaBI TAC'GTA
Cuts at: 1350 1350
Size: 4360
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4360
SphI G_CATG'C
Cuts at: 1197 1411 1911 1197
Size: 214 500 3646
Fragments arranged by size:
3646 500 214
StuI AGG'CCT
Cuts at: 489 1015 489
Size: 526 3834
StyI C'CwwG_G
Cuts at: 452 518 1175 1205 1492 1846 452
Size: 66 657 30 287 354 2966
Fragments arranged by size:
2966 657 354 287 66 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1160 1941 2074 2533 2744 1160
Size: 781 133 459 211 2776
Fragments arranged by size:
2776 781 459 211 133
TatI w'GTAC_w
Cuts at: 428 816 995 3875 428
Size: 388 179 2880 913
Fragments arranged by size:
2880 913 388 179
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3877 3909 3877
Size: 32 4328
Tth111I GACn'n_nGTC
Cuts at: 343 552 2155 343
Size: 209 1603 2548
Fragments arranged by size:
2548 1603 209
XbaI T'CTAG_A
Cuts at: 1389 1389
Size: 4360
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1015 1853 1015
Size: 838 3522
XmaI C'CCGG_G
Cuts at: 1378 1378
Size: 4360
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI
BsaXI BsiWI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII FseI FspAI MfeI NdeI NheI NotI NsiI
PacI PasI PshAI PsiI PspXI SacII SanDI SexAI
SfiI SgrAI SrfI SspI SwaI XhoI XmnI ZraI