Restriction map for pF1KSDA0832
 (Circular) (Six-base) MAPSORT of: pF1KSDA0832.seq  Check: 3620  from: 1  to: 4511



pF1KSDA0832  4511 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:34  ..

AccI GT'mk_AC

Cuts at:   1067    1499    1549    1067 
   Size:        432      50    4029

  Fragments arranged by size: 

                4029     432      50
 
Acc65I G'GTAC_C

Cuts at:    536    1527     536 
   Size:        991    3520
 
AclI AA'CG_TT

Cuts at:   4351    4351 
   Size:       4511
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    293    1282    2336    2768    3515    3783     293 
   Size:        989    1054     432     747     268    1021

  Fragments arranged by size: 

                1054    1021     989     747     432     268
 
AfeI AGC'GCT

Cuts at:   1319    1319 
   Size:       4511
 
AflIII A'CryG_T

Cuts at:   1863    2967    3944    1863 
   Size:       1104     977    2430

  Fragments arranged by size: 

                2430    1104     977
 
AgeI A'CCGG_T

Cuts at:   1070    2899    1070 
   Size:       1829    2682
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    315     347     315 
   Size:         32    4479
 
AlwNI CAG_nnn'CTG

Cuts at:   1816    3383    1816 
   Size:       1567    2944
 
ApaLI G'TGCA_C

Cuts at:   3281    3281 
   Size:       4511
 
ApoI r'AATT_y

Cuts at:   1046    1219    1515    3864    4120    1046 
   Size:        173     296    2349     256    1437

  Fragments arranged by size: 

                2349    1437     296     256     173
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4511
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4511
 
AvaI C'yCGr_G

Cuts at:   1531    1531 
   Size:       4511
 
BamHI G'GATC_C

Cuts at:   1536    1536 
   Size:       4511
 
BanI G'GyrC_C

Cuts at:    536    1527    1941    2189    2224     536 
   Size:        991     414     248      35    2823

  Fragments arranged by size: 

                2823     991     414     248      35
 
BanII G_rGCy'C

Cuts at:   1525    2555    1525 
   Size:       1030    3481
 
BbeI G_GCGC'C

Cuts at:   1945    2193    1945 
   Size:        248    4263
 
BbsI GAAGACnn'nnnn_

Cuts at:   1133    1358    1133 
   Size:        225    4286
 
BbvCI CC'TCA_GC

Cuts at:    797     797 
   Size:       4511
 
BciVI GTATCCnnnnn_n'

Cuts at:   2409    3170    4511    2409 
   Size:        761    1341    2409

  Fragments arranged by size: 

                2409    1341     761
 
BclI T'GATC_A

Cuts at:   2031    2031 
   Size:       4511
 
BglI GCCn_nnn'nGGC

Cuts at:    302     571     302 
   Size:        269    4242
 
BglII A'GATC_T

Cuts at:    869    2026    4063     869 
   Size:       1157    2037    1317

  Fragments arranged by size: 

                2037    1317    1157
 
BlpI GC'TnA_GC

Cuts at:   1623    1623 
   Size:       4511
 
Bme1580I G_kGCm'C

Cuts at:   2136    2229    3285    2136 
   Size:         93    1056    3362

  Fragments arranged by size: 

                3362    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1723    1804    2139    4283    1723 
   Size:         81     335    2144    1951

  Fragments arranged by size: 

                2144    1951     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2878    2878 
   Size:       4511
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    423     423 
   Size:       4511
 
Bpu10I CC'TnA_GC

Cuts at:    797     797 
   Size:       4511
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1687    2566    3058    3356    1687 
   Size:        879     492     298    2842

  Fragments arranged by size: 

                2842     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1079    4215    1079 
   Size:       3136    1375
 
BsaAI yAC'GTr

Cuts at:    736    1503    1864     736 
   Size:        767     361    3383

  Fragments arranged by size: 

                3383     767     361
 
BsaBI GATnn'nnATC

Cuts at:      6    2025    4062       6 
   Size:       2019    2037     455

  Fragments arranged by size: 

                2037    2019     455
 
BsaHI Gr'CG_yC

Cuts at:    314    1942    2190    2892    4388     314 
   Size:       1628     248     702    1496     437

  Fragments arranged by size: 

                1628    1496     702     437     248
 
BsaWI w'CCGG_w

Cuts at:    861    1070    1700    2221    2899    3173    3320    3731     861 
   Size:        209     630     521     678     274     147     411    1641

  Fragments arranged by size: 

                1641     678     630     521     411     274     209     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    315     345    1376    1406     315 
   Size:         30    1031      30    3420

  Fragments arranged by size: 

                3420    1031      30      30
 
BseYI C'CCAG_C

Cuts at:    261    1936    2694    3271    3767     261 
   Size:       1675     758     577     496    1005

  Fragments arranged by size: 

                1675    1005     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    151    1441     151 
   Size:       1290    3221
 
BsiEI CG_ry'CG

Cuts at:    104     945    2053    2099    3307     104 
   Size:        841    1108      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1108     841      46
 
BsiHKAI G_wGCw'C

Cuts at:   1525    2303    2493    3285    1525 
   Size:        778     190     792    2751

  Fragments arranged by size: 

                2751     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    574     574 
   Size:       4511
 
Bsp1286I G_dGCh'C

Cuts at:   1525    2136    2229    2303    2493    2555    3285    1525 
   Size:        611      93      74     190      62     730    2751

  Fragments arranged by size: 

                2751     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3731    3731 
   Size:       4511
 
BspHI T'CATG_A

Cuts at:    680    3687     680 
   Size:       3007    1504
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1561    1775    2077    2458    1561 
   Size:        214     302     381    3614

  Fragments arranged by size: 

                3614     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2803    2857    4341    4506      50 
   Size:       2753      54    1484     165      55

  Fragments arranged by size: 

                2753    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2423    2423 
   Size:       4511
 
BsrFI r'CCGG_y

Cuts at:   1070    2509    2690    2899    1070 
   Size:       1439     181     209    2682

  Fragments arranged by size: 

                2682    1439     209     181
 
BssSI C'ACGA_G

Cuts at:   2782    3140    2782 
   Size:        358    4153
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1888    1888 
   Size:       4511
 
BstBI TT'CG_AA

Cuts at:    110    2872     110 
   Size:       2762    1749
 
BstXI CCAn_nnnn'nTGG

Cuts at:    298     298 
   Size:       4511
 
BstYI r'GATC_y

Cuts at:      1     456     869    1536    2003    2026    2361    2607    3619 
   Size:        455     413     667     467      23     335     246    1012

Cuts at:   3619    3705    3717    4063       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     667     467     455     449     413     346     335
                 246      86      23      12
 
BstZ17I GTA'TAC

Cuts at:   1068    1068 
   Size:       4511
 
Bsu36I CC'TnA_GG

Cuts at:   1396    1396 
   Size:       4511
 
BtgI C'CryG_G

Cuts at:    125     125 
   Size:       4511
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    510    2461    2642    2805     510 
   Size:       1951     181     163    2216

  Fragments arranged by size: 

                2216    1951     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1384    1754    1384 
   Size:        370    4141
 
DraI TTT'AAA

Cuts at:    692    1510     692 
   Size:        818    3693
 
DraIII CAC_nnn'GTG

Cuts at:    931     931 
   Size:       4511
 
DrdI GACnn_nn'nnGTC

Cuts at:    892    2217    3075     892 
   Size:       1325     858    2328

  Fragments arranged by size: 

                2328    1325     858
 
EaeI y'GGCC_r

Cuts at:    345     938    1338    2096    2270    2661    2688    4033     345 
   Size:        593     400     758     174     391      27    1345     823

  Fragments arranged by size: 

                1345     823     758     593     400     391     174      27
            
 
EagI C'GGCC_G

Cuts at:   2096    2096 
   Size:       4511
 
EarI CTCTTCn'nnn_

Cuts at:    173     312    2534    2744     173 
   Size:        139    2222     210    1940

  Fragments arranged by size: 

                2222    1940     210     139
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1024    2719    3029    3175    4321    1024 
   Size:       1695     310     146    1146    1214

  Fragments arranged by size: 

                1695    1214    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1523    1523 
   Size:       4511
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    293     423    1282    2336    2768    3515    3783     293 
   Size:        130     859    1054     432     747     268    1021

  Fragments arranged by size: 

                1054    1021     859     747     432     268     130
 
EcoO109I rG'GnC_Cy

Cuts at:   1650    1650 
   Size:       4511
 
EcoRI G'AATT_C

Cuts at:   1515    1515 
   Size:       4511
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    594    2530     594 
   Size:       1936    2575
 
FspI TGC'GCA

Cuts at:   2292    2292 
   Size:       4511
 
HaeII r_GCGC'y

Cuts at:   1321    1945    2193    3215    4194    1321 
   Size:        624     248    1022     979    1638

  Fragments arranged by size: 

                1638    1022     979     624     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    421     453    2353    2383    2385    2415     421 
   Size:         32    1900      30       2      30    2517

  Fragments arranged by size: 

                2517    1900      32      30      30       2
 
HincII GTy'rAC

Cuts at:    285    1550     285 
   Size:       1265    3246
 
HindIII A'AGCT_T

Cuts at:   1294    1294 
   Size:       4511
 
KasI G'GCGC_C

Cuts at:   1941    2189    1941 
   Size:        248    4263
 
KpnI G_GTAC'C

Cuts at:    540    1531     540 
   Size:        991    3520
 
MluI A'CGCG_T

Cuts at:   3944    3944 
   Size:       4511
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1466    3182    3366    1466 
   Size:       1716     184    2611

  Fragments arranged by size: 

                2611    1716     184
 
MscI TGG'CCA

Cuts at:    347    1340    2272     347 
   Size:        993     932    2586

  Fragments arranged by size: 

                2586     993     932
 
MslI CAynn'nnrTG

Cuts at:   2500    2627    2500 
   Size:        127    4384
 
MspA1I CmG'CkG

Cuts at:    227     614     801    1179    1622    1936    2296    3309    3554 
   Size:        387     187     378     443     314     360    1013     245

Cuts at:   3554     227 
   Size:       1184

  Fragments arranged by size: 

                1184    1013     443     387     378     360     314     245
                 187
 
NaeI GCC'GGC

Cuts at:   2692    2692 
   Size:       4511
 
NarI GG'CG_CC

Cuts at:   1942    2190    1942 
   Size:        248    4263
 
NcoI C'CATG_G

Cuts at:    125     125 
   Size:       4511
 
NgoMIV G'CCGG_C

Cuts at:   2690    2690 
   Size:       4511
 
NspI r_CATG'y

Cuts at:    483     705    1564    2064    2971     483 
   Size:        222     859     500     907    2023

  Fragments arranged by size: 

                2023     907     859     500     222
 
PciI A'CATG_T

Cuts at:   2967    2967 
   Size:       4511
 
PflMI CCAn_nnn'nTGG

Cuts at:   1347    1347 
   Size:       4511
 
PmeI GTTT'AAAC

Cuts at:   1510    1510 
   Size:       4511
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    315     347    3650    3682     315 
   Size:         32    3303      32    1144

  Fragments arranged by size: 

                3303    1144      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1358    1358 
   Size:       4511
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    769     801     769 
   Size:         32    4479
 
PstI C_TGCA'G

Cuts at:    193    1031    1178    1325    1558    2243     193 
   Size:        838     147     147     233     685    2461

  Fragments arranged by size: 

                2461     838     685     233     147     147
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4511
 
PvuII CAG'CTG

Cuts at:    227     614     801    1179    1936    2296     227 
   Size:        387     187     378     757     360    2442

  Fragments arranged by size: 

                2442     757     387     378     360     187
 
RsrII CG'GwC_CG

Cuts at:   2706    2706 
   Size:       4511
 
SacI G_AGCT'C

Cuts at:   1525    1525 
   Size:       4511
 
SalI G'TCGA_C

Cuts at:   1548    1548 
   Size:       4511
 
SapI GCTCTTCn'nnn_

Cuts at:    173     312    2534    2744     173 
   Size:        139    2222     210    1940

  Fragments arranged by size: 

                2222    1940     210     139
 
SbfI CC_TGCA'GG

Cuts at:   1558    1558 
   Size:       4511
 
ScaI AGT'ACT

Cuts at:   4028    4028 
   Size:       4511
 
SfcI C'TryA_G

Cuts at:     33     189     327    1027    1174    1238    1321    1554    2239 
   Size:        156     138     700     147      64      83     233     685

Cuts at:   2239    3232    3423      33 
   Size:        993     191    1121

  Fragments arranged by size: 

                1121     993     700     685     233     191     156     147
                 138      83      64
 
SfoI GGC'GCC

Cuts at:   1943    2191    1943 
   Size:        248    4263
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4511
 
SmaI CCC'GGG

Cuts at:   1533    1533 
   Size:       4511
 
SmlI C'TyrA_G

Cuts at:   1666    2581    3073    3335    1666 
   Size:        915     492     262    2842

  Fragments arranged by size: 

                2842     915     492     262
 
SnaBI TAC'GTA

Cuts at:   1503    1503 
   Size:       4511
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4511
 
SphI G_CATG'C

Cuts at:    705    1564    2064     705 
   Size:        859     500    3152

  Fragments arranged by size: 

                3152     859     500
 
StuI AGG'CCT

Cuts at:   1148    1148 
   Size:       4511
 
StyI C'CwwG_G

Cuts at:    125    1056    1412    1493    1645    1999     125 
   Size:        931     356      81     152     354    2637

  Fragments arranged by size: 

                2637     931     356     354     152      81
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    959    2094    2227    2686    2895     959 
   Size:       1135     133     459     209    2575

  Fragments arranged by size: 

                2575    1135     459     209     133
 
TatI w'GTAC_w

Cuts at:    752    4026     752 
   Size:       3274    1237
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    205     237    1376    1408    4028    4060     205 
   Size:         32    1139      32    2620      32     656

  Fragments arranged by size: 

                2620    1139     656      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    280    2308     280 
   Size:       2028    2483
 
XbaI T'CTAG_A

Cuts at:   1542    1542 
   Size:       4511
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    295     298    1002    2006     295 
   Size:          3     704    1004    2800

  Fragments arranged by size: 

                2800    1004     704       3
 
XmaI C'CCGG_G

Cuts at:   1531    1531 
   Size:       4511
 
XmnI GAAnn'nnTTC

Cuts at:    182    1286     182 
   Size:       1104    3407
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AloI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PciI    PflMI     PmeI     PpiI    PshAI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AlfI     ApaI     AscI
    AvrII     BaeI     BcgI    BfrBI    BmgBI     BmtI    BseRI    BsiWI
    BsmBI    BsrGI   BssHII   BstEII     ClaI    CspCI    EcoNI    EcoRV
     FseI    FspAI     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PfoI     PmlI    PpuMI     PsiI   PspOMI
    PspXI    SacII    SanDI    SexAI     SfiI    SgrAI     SrfI     SspI
     SwaI     XhoI     ZraI
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