(Circular) (Six-base) MAPSORT of: pF1KSDA0832.seq Check: 3620 from: 1 to: 4511 pF1KSDA0832 4511 bp With 182 enzymes: SgfI * June 20, 2008 10:34 .. AccI GT'mk_AC Cuts at: 1067 1499 1549 1067 Size: 432 50 4029 Fragments arranged by size: 4029 432 50 Acc65I G'GTAC_C Cuts at: 536 1527 536 Size: 991 3520 AclI AA'CG_TT Cuts at: 4351 4351 Size: 4511 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 293 1282 2336 2768 3515 3783 293 Size: 989 1054 432 747 268 1021 Fragments arranged by size: 1054 1021 989 747 432 268 AfeI AGC'GCT Cuts at: 1319 1319 Size: 4511 AflIII A'CryG_T Cuts at: 1863 2967 3944 1863 Size: 1104 977 2430 Fragments arranged by size: 2430 1104 977 AgeI A'CCGG_T Cuts at: 1070 2899 1070 Size: 1829 2682 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 315 347 315 Size: 32 4479 AlwNI CAG_nnn'CTG Cuts at: 1816 3383 1816 Size: 1567 2944 ApaLI G'TGCA_C Cuts at: 3281 3281 Size: 4511 ApoI r'AATT_y Cuts at: 1046 1219 1515 3864 4120 1046 Size: 173 296 2349 256 1437 Fragments arranged by size: 2349 1437 296 256 173 AseI AT'TA_AT Cuts at: 20 20 Size: 4511 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4511 AvaI C'yCGr_G Cuts at: 1531 1531 Size: 4511 BamHI G'GATC_C Cuts at: 1536 1536 Size: 4511 BanI G'GyrC_C Cuts at: 536 1527 1941 2189 2224 536 Size: 991 414 248 35 2823 Fragments arranged by size: 2823 991 414 248 35 BanII G_rGCy'C Cuts at: 1525 2555 1525 Size: 1030 3481 BbeI G_GCGC'C Cuts at: 1945 2193 1945 Size: 248 4263 BbsI GAAGACnn'nnnn_ Cuts at: 1133 1358 1133 Size: 225 4286 BbvCI CC'TCA_GC Cuts at: 797 797 Size: 4511 BciVI GTATCCnnnnn_n' Cuts at: 2409 3170 4511 2409 Size: 761 1341 2409 Fragments arranged by size: 2409 1341 761 BclI T'GATC_A Cuts at: 2031 2031 Size: 4511 BglI GCCn_nnn'nGGC Cuts at: 302 571 302 Size: 269 4242 BglII A'GATC_T Cuts at: 869 2026 4063 869 Size: 1157 2037 1317 Fragments arranged by size: 2037 1317 1157 BlpI GC'TnA_GC Cuts at: 1623 1623 Size: 4511 Bme1580I G_kGCm'C Cuts at: 2136 2229 3285 2136 Size: 93 1056 3362 Fragments arranged by size: 3362 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1723 1804 2139 4283 1723 Size: 81 335 2144 1951 Fragments arranged by size: 2144 1951 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2878 2878 Size: 4511 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 423 423 Size: 4511 Bpu10I CC'TnA_GC Cuts at: 797 797 Size: 4511 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1687 2566 3058 3356 1687 Size: 879 492 298 2842 Fragments arranged by size: 2842 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 1079 4215 1079 Size: 3136 1375 BsaAI yAC'GTr Cuts at: 736 1503 1864 736 Size: 767 361 3383 Fragments arranged by size: 3383 767 361 BsaBI GATnn'nnATC Cuts at: 6 2025 4062 6 Size: 2019 2037 455 Fragments arranged by size: 2037 2019 455 BsaHI Gr'CG_yC Cuts at: 314 1942 2190 2892 4388 314 Size: 1628 248 702 1496 437 Fragments arranged by size: 1628 1496 702 437 248 BsaWI w'CCGG_w Cuts at: 861 1070 1700 2221 2899 3173 3320 3731 861 Size: 209 630 521 678 274 147 411 1641 Fragments arranged by size: 1641 678 630 521 411 274 209 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 315 345 1376 1406 315 Size: 30 1031 30 3420 Fragments arranged by size: 3420 1031 30 30 BseYI C'CCAG_C Cuts at: 261 1936 2694 3271 3767 261 Size: 1675 758 577 496 1005 Fragments arranged by size: 1675 1005 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 151 1441 151 Size: 1290 3221 BsiEI CG_ry'CG Cuts at: 104 945 2053 2099 3307 104 Size: 841 1108 46 1208 1308 Fragments arranged by size: 1308 1208 1108 841 46 BsiHKAI G_wGCw'C Cuts at: 1525 2303 2493 3285 1525 Size: 778 190 792 2751 Fragments arranged by size: 2751 792 778 190 BsmI GAATG_Cn' Cuts at: 574 574 Size: 4511 Bsp1286I G_dGCh'C Cuts at: 1525 2136 2229 2303 2493 2555 3285 1525 Size: 611 93 74 190 62 730 2751 Fragments arranged by size: 2751 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3731 3731 Size: 4511 BspHI T'CATG_A Cuts at: 680 3687 680 Size: 3007 1504 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1561 1775 2077 2458 1561 Size: 214 302 381 3614 Fragments arranged by size: 3614 381 302 214 BsrBI CCG'CTC Cuts at: 50 2803 2857 4341 4506 50 Size: 2753 54 1484 165 55 Fragments arranged by size: 2753 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2423 2423 Size: 4511 BsrFI r'CCGG_y Cuts at: 1070 2509 2690 2899 1070 Size: 1439 181 209 2682 Fragments arranged by size: 2682 1439 209 181 BssSI C'ACGA_G Cuts at: 2782 3140 2782 Size: 358 4153 BstAPI GCAn_nnn'nTGC Cuts at: 1888 1888 Size: 4511 BstBI TT'CG_AA Cuts at: 110 2872 110 Size: 2762 1749 BstXI CCAn_nnnn'nTGG Cuts at: 298 298 Size: 4511 BstYI r'GATC_y Cuts at: 1 456 869 1536 2003 2026 2361 2607 3619 Size: 455 413 667 467 23 335 246 1012 Cuts at: 3619 3705 3717 4063 1 Size: 86 12 346 449 Fragments arranged by size: 1012 667 467 455 449 413 346 335 246 86 23 12 BstZ17I GTA'TAC Cuts at: 1068 1068 Size: 4511 Bsu36I CC'TnA_GG Cuts at: 1396 1396 Size: 4511 BtgI C'CryG_G Cuts at: 125 125 Size: 4511 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 510 2461 2642 2805 510 Size: 1951 181 163 2216 Fragments arranged by size: 2216 1951 181 163 BtsI GCAGTG_nn' Cuts at: 1384 1754 1384 Size: 370 4141 DraI TTT'AAA Cuts at: 692 1510 692 Size: 818 3693 DraIII CAC_nnn'GTG Cuts at: 931 931 Size: 4511 DrdI GACnn_nn'nnGTC Cuts at: 892 2217 3075 892 Size: 1325 858 2328 Fragments arranged by size: 2328 1325 858 EaeI y'GGCC_r Cuts at: 345 938 1338 2096 2270 2661 2688 4033 345 Size: 593 400 758 174 391 27 1345 823 Fragments arranged by size: 1345 823 758 593 400 391 174 27 EagI C'GGCC_G Cuts at: 2096 2096 Size: 4511 EarI CTCTTCn'nnn_ Cuts at: 173 312 2534 2744 173 Size: 139 2222 210 1940 Fragments arranged by size: 2222 1940 210 139 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1024 2719 3029 3175 4321 1024 Size: 1695 310 146 1146 1214 Fragments arranged by size: 1695 1214 1146 310 146 EcoICRI GAG'CTC Cuts at: 1523 1523 Size: 4511 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 293 423 1282 2336 2768 3515 3783 293 Size: 130 859 1054 432 747 268 1021 Fragments arranged by size: 1054 1021 859 747 432 268 130 EcoO109I rG'GnC_Cy Cuts at: 1650 1650 Size: 4511 EcoRI G'AATT_C Cuts at: 1515 1515 Size: 4511 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 594 2530 594 Size: 1936 2575 FspI TGC'GCA Cuts at: 2292 2292 Size: 4511 HaeII r_GCGC'y Cuts at: 1321 1945 2193 3215 4194 1321 Size: 624 248 1022 979 1638 Fragments arranged by size: 1638 1022 979 624 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 421 453 2353 2383 2385 2415 421 Size: 32 1900 30 2 30 2517 Fragments arranged by size: 2517 1900 32 30 30 2 HincII GTy'rAC Cuts at: 285 1550 285 Size: 1265 3246 HindIII A'AGCT_T Cuts at: 1294 1294 Size: 4511 KasI G'GCGC_C Cuts at: 1941 2189 1941 Size: 248 4263 KpnI G_GTAC'C Cuts at: 540 1531 540 Size: 991 3520 MluI A'CGCG_T Cuts at: 3944 3944 Size: 4511 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1466 3182 3366 1466 Size: 1716 184 2611 Fragments arranged by size: 2611 1716 184 MscI TGG'CCA Cuts at: 347 1340 2272 347 Size: 993 932 2586 Fragments arranged by size: 2586 993 932 MslI CAynn'nnrTG Cuts at: 2500 2627 2500 Size: 127 4384 MspA1I CmG'CkG Cuts at: 227 614 801 1179 1622 1936 2296 3309 3554 Size: 387 187 378 443 314 360 1013 245 Cuts at: 3554 227 Size: 1184 Fragments arranged by size: 1184 1013 443 387 378 360 314 245 187 NaeI GCC'GGC Cuts at: 2692 2692 Size: 4511 NarI GG'CG_CC Cuts at: 1942 2190 1942 Size: 248 4263 NcoI C'CATG_G Cuts at: 125 125 Size: 4511 NgoMIV G'CCGG_C Cuts at: 2690 2690 Size: 4511 NspI r_CATG'y Cuts at: 483 705 1564 2064 2971 483 Size: 222 859 500 907 2023 Fragments arranged by size: 2023 907 859 500 222 PciI A'CATG_T Cuts at: 2967 2967 Size: 4511 PflMI CCAn_nnn'nTGG Cuts at: 1347 1347 Size: 4511 PmeI GTTT'AAAC Cuts at: 1510 1510 Size: 4511 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 315 347 3650 3682 315 Size: 32 3303 32 1144 Fragments arranged by size: 3303 1144 32 32 PshAI GACnn'nnGTC Cuts at: 1358 1358 Size: 4511 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 769 801 769 Size: 32 4479 PstI C_TGCA'G Cuts at: 193 1031 1178 1325 1558 2243 193 Size: 838 147 147 233 685 2461 Fragments arranged by size: 2461 838 685 233 147 147 PvuI CG_AT'CG Cuts at: 104 104 Size: 4511 PvuII CAG'CTG Cuts at: 227 614 801 1179 1936 2296 227 Size: 387 187 378 757 360 2442 Fragments arranged by size: 2442 757 387 378 360 187 RsrII CG'GwC_CG Cuts at: 2706 2706 Size: 4511 SacI G_AGCT'C Cuts at: 1525 1525 Size: 4511 SalI G'TCGA_C Cuts at: 1548 1548 Size: 4511 SapI GCTCTTCn'nnn_ Cuts at: 173 312 2534 2744 173 Size: 139 2222 210 1940 Fragments arranged by size: 2222 1940 210 139 SbfI CC_TGCA'GG Cuts at: 1558 1558 Size: 4511 ScaI AGT'ACT Cuts at: 4028 4028 Size: 4511 SfcI C'TryA_G Cuts at: 33 189 327 1027 1174 1238 1321 1554 2239 Size: 156 138 700 147 64 83 233 685 Cuts at: 2239 3232 3423 33 Size: 993 191 1121 Fragments arranged by size: 1121 993 700 685 233 191 156 147 138 83 64 SfoI GGC'GCC Cuts at: 1943 2191 1943 Size: 248 4263 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4511 SmaI CCC'GGG Cuts at: 1533 1533 Size: 4511 SmlI C'TyrA_G Cuts at: 1666 2581 3073 3335 1666 Size: 915 492 262 2842 Fragments arranged by size: 2842 915 492 262 SnaBI TAC'GTA Cuts at: 1503 1503 Size: 4511 SpeI A'CTAG_T Cuts at: 67 67 Size: 4511 SphI G_CATG'C Cuts at: 705 1564 2064 705 Size: 859 500 3152 Fragments arranged by size: 3152 859 500 StuI AGG'CCT Cuts at: 1148 1148 Size: 4511 StyI C'CwwG_G Cuts at: 125 1056 1412 1493 1645 1999 125 Size: 931 356 81 152 354 2637 Fragments arranged by size: 2637 931 356 354 152 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 959 2094 2227 2686 2895 959 Size: 1135 133 459 209 2575 Fragments arranged by size: 2575 1135 459 209 133 TatI w'GTAC_w Cuts at: 752 4026 752 Size: 3274 1237 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 205 237 1376 1408 4028 4060 205 Size: 32 1139 32 2620 32 656 Fragments arranged by size: 2620 1139 656 32 32 32 Tth111I GACn'n_nGTC Cuts at: 280 2308 280 Size: 2028 2483 XbaI T'CTAG_A Cuts at: 1542 1542 Size: 4511 XcmI CCAnnnn_n'nnnnTGG Cuts at: 295 298 1002 2006 295 Size: 3 704 1004 2800 Fragments arranged by size: 2800 1004 704 3 XmaI C'CCGG_G Cuts at: 1531 1531 Size: 4511 XmnI GAAnn'nnTTC Cuts at: 182 1286 182 Size: 1104 3407 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI PshAI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AflII AhdI AleI AlfI ApaI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BseRI BsiWI BsmBI BsrGI BssHII BstEII ClaI CspCI EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmlI PpuMI PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SrfI SspI SwaI XhoI ZraI