Restriction map for
pF1KSDA0832
(Circular) (Six-base) MAPSORT of: pF1KSDA0832.seq Check: 3620 from: 1 to: 4511
pF1KSDA0832 4511 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 1067 1499 1549 1067
Size: 432 50 4029
Fragments arranged by size:
4029 432 50
Acc65I G'GTAC_C
Cuts at: 536 1527 536
Size: 991 3520
AclI AA'CG_TT
Cuts at: 4351 4351
Size: 4511
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 1282 2336 2768 3515 3783 293
Size: 989 1054 432 747 268 1021
Fragments arranged by size:
1054 1021 989 747 432 268
AfeI AGC'GCT
Cuts at: 1319 1319
Size: 4511
AflIII A'CryG_T
Cuts at: 1863 2967 3944 1863
Size: 1104 977 2430
Fragments arranged by size:
2430 1104 977
AgeI A'CCGG_T
Cuts at: 1070 2899 1070
Size: 1829 2682
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 315 347 315
Size: 32 4479
AlwNI CAG_nnn'CTG
Cuts at: 1816 3383 1816
Size: 1567 2944
ApaLI G'TGCA_C
Cuts at: 3281 3281
Size: 4511
ApoI r'AATT_y
Cuts at: 1046 1219 1515 3864 4120 1046
Size: 173 296 2349 256 1437
Fragments arranged by size:
2349 1437 296 256 173
AseI AT'TA_AT
Cuts at: 20 20
Size: 4511
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4511
AvaI C'yCGr_G
Cuts at: 1531 1531
Size: 4511
BamHI G'GATC_C
Cuts at: 1536 1536
Size: 4511
BanI G'GyrC_C
Cuts at: 536 1527 1941 2189 2224 536
Size: 991 414 248 35 2823
Fragments arranged by size:
2823 991 414 248 35
BanII G_rGCy'C
Cuts at: 1525 2555 1525
Size: 1030 3481
BbeI G_GCGC'C
Cuts at: 1945 2193 1945
Size: 248 4263
BbsI GAAGACnn'nnnn_
Cuts at: 1133 1358 1133
Size: 225 4286
BbvCI CC'TCA_GC
Cuts at: 797 797
Size: 4511
BciVI GTATCCnnnnn_n'
Cuts at: 2409 3170 4511 2409
Size: 761 1341 2409
Fragments arranged by size:
2409 1341 761
BclI T'GATC_A
Cuts at: 2031 2031
Size: 4511
BglI GCCn_nnn'nGGC
Cuts at: 302 571 302
Size: 269 4242
BglII A'GATC_T
Cuts at: 869 2026 4063 869
Size: 1157 2037 1317
Fragments arranged by size:
2037 1317 1157
BlpI GC'TnA_GC
Cuts at: 1623 1623
Size: 4511
Bme1580I G_kGCm'C
Cuts at: 2136 2229 3285 2136
Size: 93 1056 3362
Fragments arranged by size:
3362 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1723 1804 2139 4283 1723
Size: 81 335 2144 1951
Fragments arranged by size:
2144 1951 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2878 2878
Size: 4511
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 423 423
Size: 4511
Bpu10I CC'TnA_GC
Cuts at: 797 797
Size: 4511
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1687 2566 3058 3356 1687
Size: 879 492 298 2842
Fragments arranged by size:
2842 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1079 4215 1079
Size: 3136 1375
BsaAI yAC'GTr
Cuts at: 736 1503 1864 736
Size: 767 361 3383
Fragments arranged by size:
3383 767 361
BsaBI GATnn'nnATC
Cuts at: 6 2025 4062 6
Size: 2019 2037 455
Fragments arranged by size:
2037 2019 455
BsaHI Gr'CG_yC
Cuts at: 314 1942 2190 2892 4388 314
Size: 1628 248 702 1496 437
Fragments arranged by size:
1628 1496 702 437 248
BsaWI w'CCGG_w
Cuts at: 861 1070 1700 2221 2899 3173 3320 3731 861
Size: 209 630 521 678 274 147 411 1641
Fragments arranged by size:
1641 678 630 521 411 274 209 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 315 345 1376 1406 315
Size: 30 1031 30 3420
Fragments arranged by size:
3420 1031 30 30
BseYI C'CCAG_C
Cuts at: 261 1936 2694 3271 3767 261
Size: 1675 758 577 496 1005
Fragments arranged by size:
1675 1005 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 1441 151
Size: 1290 3221
BsiEI CG_ry'CG
Cuts at: 104 945 2053 2099 3307 104
Size: 841 1108 46 1208 1308
Fragments arranged by size:
1308 1208 1108 841 46
BsiHKAI G_wGCw'C
Cuts at: 1525 2303 2493 3285 1525
Size: 778 190 792 2751
Fragments arranged by size:
2751 792 778 190
BsmI GAATG_Cn'
Cuts at: 574 574
Size: 4511
Bsp1286I G_dGCh'C
Cuts at: 1525 2136 2229 2303 2493 2555 3285 1525
Size: 611 93 74 190 62 730 2751
Fragments arranged by size:
2751 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3731 3731
Size: 4511
BspHI T'CATG_A
Cuts at: 680 3687 680
Size: 3007 1504
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1561 1775 2077 2458 1561
Size: 214 302 381 3614
Fragments arranged by size:
3614 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2803 2857 4341 4506 50
Size: 2753 54 1484 165 55
Fragments arranged by size:
2753 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2423 2423
Size: 4511
BsrFI r'CCGG_y
Cuts at: 1070 2509 2690 2899 1070
Size: 1439 181 209 2682
Fragments arranged by size:
2682 1439 209 181
BssSI C'ACGA_G
Cuts at: 2782 3140 2782
Size: 358 4153
BstAPI GCAn_nnn'nTGC
Cuts at: 1888 1888
Size: 4511
BstBI TT'CG_AA
Cuts at: 110 2872 110
Size: 2762 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 298 298
Size: 4511
BstYI r'GATC_y
Cuts at: 1 456 869 1536 2003 2026 2361 2607 3619
Size: 455 413 667 467 23 335 246 1012
Cuts at: 3619 3705 3717 4063 1
Size: 86 12 346 449
Fragments arranged by size:
1012 667 467 455 449 413 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1068 1068
Size: 4511
Bsu36I CC'TnA_GG
Cuts at: 1396 1396
Size: 4511
BtgI C'CryG_G
Cuts at: 125 125
Size: 4511
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 510 2461 2642 2805 510
Size: 1951 181 163 2216
Fragments arranged by size:
2216 1951 181 163
BtsI GCAGTG_nn'
Cuts at: 1384 1754 1384
Size: 370 4141
DraI TTT'AAA
Cuts at: 692 1510 692
Size: 818 3693
DraIII CAC_nnn'GTG
Cuts at: 931 931
Size: 4511
DrdI GACnn_nn'nnGTC
Cuts at: 892 2217 3075 892
Size: 1325 858 2328
Fragments arranged by size:
2328 1325 858
EaeI y'GGCC_r
Cuts at: 345 938 1338 2096 2270 2661 2688 4033 345
Size: 593 400 758 174 391 27 1345 823
Fragments arranged by size:
1345 823 758 593 400 391 174 27
EagI C'GGCC_G
Cuts at: 2096 2096
Size: 4511
EarI CTCTTCn'nnn_
Cuts at: 173 312 2534 2744 173
Size: 139 2222 210 1940
Fragments arranged by size:
2222 1940 210 139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1024 2719 3029 3175 4321 1024
Size: 1695 310 146 1146 1214
Fragments arranged by size:
1695 1214 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1523 1523
Size: 4511
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 423 1282 2336 2768 3515 3783 293
Size: 130 859 1054 432 747 268 1021
Fragments arranged by size:
1054 1021 859 747 432 268 130
EcoO109I rG'GnC_Cy
Cuts at: 1650 1650
Size: 4511
EcoRI G'AATT_C
Cuts at: 1515 1515
Size: 4511
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 594 2530 594
Size: 1936 2575
FspI TGC'GCA
Cuts at: 2292 2292
Size: 4511
HaeII r_GCGC'y
Cuts at: 1321 1945 2193 3215 4194 1321
Size: 624 248 1022 979 1638
Fragments arranged by size:
1638 1022 979 624 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 421 453 2353 2383 2385 2415 421
Size: 32 1900 30 2 30 2517
Fragments arranged by size:
2517 1900 32 30 30 2
HincII GTy'rAC
Cuts at: 285 1550 285
Size: 1265 3246
HindIII A'AGCT_T
Cuts at: 1294 1294
Size: 4511
KasI G'GCGC_C
Cuts at: 1941 2189 1941
Size: 248 4263
KpnI G_GTAC'C
Cuts at: 540 1531 540
Size: 991 3520
MluI A'CGCG_T
Cuts at: 3944 3944
Size: 4511
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1466 3182 3366 1466
Size: 1716 184 2611
Fragments arranged by size:
2611 1716 184
MscI TGG'CCA
Cuts at: 347 1340 2272 347
Size: 993 932 2586
Fragments arranged by size:
2586 993 932
MslI CAynn'nnrTG
Cuts at: 2500 2627 2500
Size: 127 4384
MspA1I CmG'CkG
Cuts at: 227 614 801 1179 1622 1936 2296 3309 3554
Size: 387 187 378 443 314 360 1013 245
Cuts at: 3554 227
Size: 1184
Fragments arranged by size:
1184 1013 443 387 378 360 314 245
187
NaeI GCC'GGC
Cuts at: 2692 2692
Size: 4511
NarI GG'CG_CC
Cuts at: 1942 2190 1942
Size: 248 4263
NcoI C'CATG_G
Cuts at: 125 125
Size: 4511
NgoMIV G'CCGG_C
Cuts at: 2690 2690
Size: 4511
NspI r_CATG'y
Cuts at: 483 705 1564 2064 2971 483
Size: 222 859 500 907 2023
Fragments arranged by size:
2023 907 859 500 222
PciI A'CATG_T
Cuts at: 2967 2967
Size: 4511
PflMI CCAn_nnn'nTGG
Cuts at: 1347 1347
Size: 4511
PmeI GTTT'AAAC
Cuts at: 1510 1510
Size: 4511
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 315 347 3650 3682 315
Size: 32 3303 32 1144
Fragments arranged by size:
3303 1144 32 32
PshAI GACnn'nnGTC
Cuts at: 1358 1358
Size: 4511
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 769 801 769
Size: 32 4479
PstI C_TGCA'G
Cuts at: 193 1031 1178 1325 1558 2243 193
Size: 838 147 147 233 685 2461
Fragments arranged by size:
2461 838 685 233 147 147
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4511
PvuII CAG'CTG
Cuts at: 227 614 801 1179 1936 2296 227
Size: 387 187 378 757 360 2442
Fragments arranged by size:
2442 757 387 378 360 187
RsrII CG'GwC_CG
Cuts at: 2706 2706
Size: 4511
SacI G_AGCT'C
Cuts at: 1525 1525
Size: 4511
SalI G'TCGA_C
Cuts at: 1548 1548
Size: 4511
SapI GCTCTTCn'nnn_
Cuts at: 173 312 2534 2744 173
Size: 139 2222 210 1940
Fragments arranged by size:
2222 1940 210 139
SbfI CC_TGCA'GG
Cuts at: 1558 1558
Size: 4511
ScaI AGT'ACT
Cuts at: 4028 4028
Size: 4511
SfcI C'TryA_G
Cuts at: 33 189 327 1027 1174 1238 1321 1554 2239
Size: 156 138 700 147 64 83 233 685
Cuts at: 2239 3232 3423 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 700 685 233 191 156 147
138 83 64
SfoI GGC'GCC
Cuts at: 1943 2191 1943
Size: 248 4263
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4511
SmaI CCC'GGG
Cuts at: 1533 1533
Size: 4511
SmlI C'TyrA_G
Cuts at: 1666 2581 3073 3335 1666
Size: 915 492 262 2842
Fragments arranged by size:
2842 915 492 262
SnaBI TAC'GTA
Cuts at: 1503 1503
Size: 4511
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4511
SphI G_CATG'C
Cuts at: 705 1564 2064 705
Size: 859 500 3152
Fragments arranged by size:
3152 859 500
StuI AGG'CCT
Cuts at: 1148 1148
Size: 4511
StyI C'CwwG_G
Cuts at: 125 1056 1412 1493 1645 1999 125
Size: 931 356 81 152 354 2637
Fragments arranged by size:
2637 931 356 354 152 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 959 2094 2227 2686 2895 959
Size: 1135 133 459 209 2575
Fragments arranged by size:
2575 1135 459 209 133
TatI w'GTAC_w
Cuts at: 752 4026 752
Size: 3274 1237
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 205 237 1376 1408 4028 4060 205
Size: 32 1139 32 2620 32 656
Fragments arranged by size:
2620 1139 656 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 280 2308 280
Size: 2028 2483
XbaI T'CTAG_A
Cuts at: 1542 1542
Size: 4511
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 295 298 1002 2006 295
Size: 3 704 1004 2800
Fragments arranged by size:
2800 1004 704 3
XmaI C'CCGG_G
Cuts at: 1531 1531
Size: 4511
XmnI GAAnn'nnTTC
Cuts at: 182 1286 182
Size: 1104 3407
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PshAI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BseRI BsiWI
BsmBI BsrGI BssHII BstEII ClaI CspCI EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PsiI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SrfI SspI
SwaI XhoI ZraI