Restriction map for
pF1KSDA0821
(Circular) (Six-base) MAPSORT of: pF1KSDA0821.seq Check: 1349 from: 1 to: 7559
pF1KSDA0821 7559 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2046 3686 2046
Size: 1640 5919
AatII G_ACGT'C
Cuts at: 290 1835 2087 290
Size: 1545 252 5762
Fragments arranged by size:
5762 1545 252
AccI GT'mk_AC
Cuts at: 779 1671 2575 4597 779
Size: 892 904 2022 3741
Fragments arranged by size:
3741 2022 904 892
Acc65I G'GTAC_C
Cuts at: 3661 4575 3661
Size: 914 6645
AclI AA'CG_TT
Cuts at: 7399 7399
Size: 7559
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 5384 5816 6563 6831 5384
Size: 432 747 268 6112
Fragments arranged by size:
6112 747 432 268
AfeI AGC'GCT
Cuts at: 1063 1063
Size: 7559
AflIII A'CryG_T
Cuts at: 1542 1544 2620 4911 6015 6992 1542
Size: 2 1076 2291 1104 977 2109
Fragments arranged by size:
2291 2109 1104 1076 977 2
AgeI A'CCGG_T
Cuts at: 5947 5947
Size: 7559
AleI CACnn'nnGTG
Cuts at: 408 2719 408
Size: 2311 5248
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3475 3475
Size: 7559
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2197 2229 2197
Size: 32 7527
AlwNI CAG_nnn'CTG
Cuts at: 1718 1866 1896 2208 4864 6431 1718
Size: 148 30 312 2656 1567 2846
Fragments arranged by size:
2846 2656 1567 312 148 30
ApaI G_GGCC'C
Cuts at: 1705 3922 4033 4100 4173 4340 4511 1705
Size: 2217 111 67 73 167 171 4753
Fragments arranged by size:
4753 2217 171 167 111 73 67
ApaLI G'TGCA_C
Cuts at: 2059 2962 3436 3991 6329 2059
Size: 903 474 555 2338 3289
Fragments arranged by size:
3289 2338 903 555 474
ApoI r'AATT_y
Cuts at: 3569 4563 6912 7168 3569
Size: 994 2349 256 3960
Fragments arranged by size:
3960 2349 994 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7559
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7559
AvaI C'yCGr_G
Cuts at: 1571 1646 1697 1808 2232 2382 3493 3907 3932
Size: 75 51 111 424 150 1111 414 25
Cuts at: 3932 4099 4166 4579 1571
Size: 167 67 413 4551
Fragments arranged by size:
4551 1111 424 414 413 167 150 111
75 67 51 25
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3645 3678 3645
Size: 33 7526
BamHI G'GATC_C
Cuts at: 662 3508 4584 662
Size: 2846 1076 3637
Fragments arranged by size:
3637 2846 1076
BanI G'GyrC_C
Cuts at: 272 787 1434 1459 2098 2398 3094 3661 3712
Size: 515 647 25 639 300 696 567 51
Cuts at: 3712 4231 4575 4989 5237 5272 272
Size: 519 344 414 248 35 2559
Fragments arranged by size:
2559 696 647 639 567 519 515 414
344 300 248 51 35 25
BanII G_rGCy'C
Cuts at: 215 575 1559 1571 1688 1705 1816 3922 4033
Size: 360 984 12 117 17 111 2106 111
Cuts at: 4033 4049 4100 4173 4340 4511 4573 5603 215
Size: 16 51 73 167 171 62 1030 2171
Fragments arranged by size:
2171 2106 1030 984 360 171 167 117
111 111 73 62 51 17 16 12
BbeI G_GCGC'C
Cuts at: 2102 2402 4235 4993 5241 2102
Size: 300 1833 758 248 4420
Fragments arranged by size:
4420 1833 758 300 248
BbsI GAAGACnn'nnnn_
Cuts at: 528 786 2298 2454 3390 3768 528
Size: 258 1512 156 936 378 4319
Fragments arranged by size:
4319 1512 936 378 258 156
BbvCI CC'TCA_GC
Cuts at: 1357 1711 3522 1357
Size: 354 1811 5394
Fragments arranged by size:
5394 1811 354
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1312 1346 1312
Size: 34 7525
BciVI GTATCCnnnnn_n'
Cuts at: 5457 6218 7559 5457
Size: 761 1341 5457
Fragments arranged by size:
5457 1341 761
BclI T'GATC_A
Cuts at: 3974 5079 3974
Size: 1105 6454
BglI GCCn_nnn'nGGC
Cuts at: 2387 2396 2957 3955 4288 4420 4472 2387
Size: 9 561 998 333 132 52 5474
Fragments arranged by size:
5474 998 561 333 132 52 9
BglII A'GATC_T
Cuts at: 2672 5074 7111 2672
Size: 2402 2037 3120
Fragments arranged by size:
3120 2402 2037
BlpI GC'TnA_GC
Cuts at: 2843 4671 2843
Size: 1828 5731
Bme1580I G_kGCm'C
Cuts at: 277 547 1039 1437 1705 2063 2966 3440 3502
Size: 270 492 398 268 358 903 474 62
Cuts at: 3502 3715 3922 3995 4033 4100 4173 4340 4511
Size: 213 207 73 38 67 73 167 171
Cuts at: 4511 5184 5277 6333 277
Size: 673 93 1056 1503
Fragments arranged by size:
1503 1056 903 673 492 474 398 358
270 268 213 207 171 167 93 73
73 67 62 38
BmrI ACTGGGnnnn_n'
Cuts at: 420 1322 1447 1498 4167 4334 4771 4852 5187
Size: 902 125 51 2669 167 437 81 335
Cuts at: 5187 7331 420
Size: 2144 648
Fragments arranged by size:
2669 2144 902 648 437 335 167 125
81 51
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 594 714 5926 594
Size: 120 5212 2227
Fragments arranged by size:
5212 2227 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 589 1327 1475 2044 2110 2188 2521 2974
Size: 78 738 148 569 66 78 333 453
Cuts at: 2974 3159 3228 4165 511
Size: 185 69 937 3905
Fragments arranged by size:
3905 937 738 569 453 333 185 148
78 78 69 66
Bpu10I CC'TnA_GC
Cuts at: 199 1357 1553 1711 3522 3923 4043 199
Size: 1158 196 158 1811 401 120 3715
Fragments arranged by size:
3715 1811 1158 401 196 158 120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3217 3499 4516 4735 5614 6106 6404 3217
Size: 282 1017 219 879 492 298 4372
Fragments arranged by size:
4372 1017 879 492 298 282 219
BsaI GGTCTCn'nnnn_
Cuts at: 7263 7263
Size: 7559
BsaAI yAC'GTr
Cuts at: 651 714 1040 1122 1545 2621 4551 4912 651
Size: 63 326 82 423 1076 1930 361 3298
Fragments arranged by size:
3298 1930 1076 423 361 326 82 63
BsaBI GATnn'nnATC
Cuts at: 6 3976 5073 7110 6
Size: 3970 1097 2037 455
Fragments arranged by size:
3970 2037 1097 455
BsaHI Gr'CG_yC
Cuts at: 287 386 1832 2084 2099 2399 4232 4458 4990
Size: 99 1446 252 15 300 1833 226 532
Cuts at: 4990 5238 5940 7436 287
Size: 248 702 1496 410
Fragments arranged by size:
1833 1496 1446 702 532 410 300 252
248 226 99 15
BsaWI w'CCGG_w
Cuts at: 4748 5269 5947 6221 6368 6779 4748
Size: 521 678 274 147 411 5528
Fragments arranged by size:
5528 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 229 259 484 514 1708 1738 1822 1852 2197
Size: 30 225 30 1194 30 84 30 345
Cuts at: 2197 2227 3865 3895 4294 4324 229
Size: 30 1638 30 399 30 3464
Fragments arranged by size:
3464 1638 1194 399 345 225 84 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1204 1224 1827 1830 2068 2107 2239 3319 3753
Size: 20 603 3 238 39 132 1080 434
Cuts at: 3753 4162 4242 1204
Size: 409 80 4521
Fragments arranged by size:
4521 1080 603 434 409 238 132 80
39 20 3
BseYI C'CCAG_C
Cuts at: 908 1338 1473 1510 1527 1604 1883 2562 3276
Size: 430 135 37 17 77 279 679 714
Cuts at: 3276 3303 4984 5742 6319 6815 908
Size: 27 1681 758 577 496 1652
Fragments arranged by size:
1681 1652 758 714 679 577 496 430
279 135 77 37 27 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 517 1704 2221 2899 3187 4200 388
Size: 129 1187 517 678 288 1013 3747
Fragments arranged by size:
3747 1187 1013 678 517 288 129
BsiEI CG_ry'CG
Cuts at: 104 2799 4223 5101 5147 6355 104
Size: 2695 1424 878 46 1208 1308
Fragments arranged by size:
2695 1424 1308 1208 878 46
BsiHKAI G_wGCw'C
Cuts at: 149 599 1559 2063 2657 2966 3233 3440 3995
Size: 450 960 504 594 309 267 207 555
Cuts at: 3995 4573 5351 5541 6333 149
Size: 578 778 190 792 1375
Fragments arranged by size:
1375 960 792 778 594 578 555 504
450 309 267 207 190
BsmI GAATG_Cn'
Cuts at: 309 2049 2355 309
Size: 1740 306 5513
Fragments arranged by size:
5513 1740 306
BsmBI CGTCTCn'nnnn_
Cuts at: 856 1039 1839 856
Size: 183 800 6576
Fragments arranged by size:
6576 800 183
Bsp1286I G_dGCh'C
Cuts at: 149 215 277 547 575 599 1039 1437 1559
Size: 66 62 270 28 24 440 398 122
Cuts at: 1559 1571 1688 1705 1816 2063 2657 2966 3233
Size: 12 117 17 111 247 594 309 267
Cuts at: 3233 3440 3502 3715 3922 3995 4033 4049 4100
Size: 207 62 213 207 73 38 16 51
Cuts at: 4100 4173 4340 4511 4573 5184 5277 5351 5541
Size: 73 167 171 62 611 93 74 190
Cuts at: 5541 5603 6333 149
Size: 62 730 1375
Fragments arranged by size:
1375 730 611 594 440 398 309 270
267 247 213 207 207 190 171 167
122 117 111 93 74 73 73 66
62 62 62 62 51 38 28 24
17 16 12
BspEI T'CCGG_A
Cuts at: 6779 6779
Size: 7559
BspHI T'CATG_A
Cuts at: 6735 6735
Size: 7559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 386 2046 2981 3686 4010 4304 4609 4823 5125
Size: 1660 935 705 324 294 305 214 302
Cuts at: 5125 5506 386
Size: 381 2439
Fragments arranged by size:
2439 1660 935 705 381 324 305 302
294 214
BsrBI CCG'CTC
Cuts at: 50 857 1425 3485 5851 5905 7389 7554 50
Size: 807 568 2060 2366 54 1484 165 55
Fragments arranged by size:
2366 2060 1484 807 568 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2292 5471 2292
Size: 3179 4380
BsrFI r'CCGG_y
Cuts at: 442 1596 1921 2368 2905 4241 5557 5738 5947
Size: 1154 325 447 537 1336 1316 181 209
Cuts at: 5947 442
Size: 2054
Fragments arranged by size:
2054 1336 1316 1154 537 447 325 209
181
BssSI C'ACGA_G
Cuts at: 586 699 4468 5830 6188 586
Size: 113 3769 1362 358 1957
Fragments arranged by size:
3769 1957 1362 358 113
BstAPI GCAn_nnn'nTGC
Cuts at: 428 564 1614 1896 4936 428
Size: 136 1050 282 3040 3051
Fragments arranged by size:
3051 3040 1050 282 136
BstBI TT'CG_AA
Cuts at: 110 5920 110
Size: 5810 1749
BstEII G'GTnAC_C
Cuts at: 177 637 3195 4536 177
Size: 460 2558 1341 3200
Fragments arranged by size:
3200 2558 1341 460
BstXI CCAn_nnnn'nTGG
Cuts at: 756 1794 3252 756
Size: 1038 1458 5063
Fragments arranged by size:
5063 1458 1038
BstYI r'GATC_y
Cuts at: 1 662 2672 3508 4584 5051 5074 5409 5655
Size: 661 2010 836 1076 467 23 335 246
Cuts at: 5655 6667 6753 6765 7111 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2010 1076 1012 836 661 467 449 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 4331 4331
Size: 7559
BtgI C'CryG_G
Cuts at: 125 1982 2048 2342 2489 3665 3705 125
Size: 1857 66 294 147 1176 40 3979
Fragments arranged by size:
3979 1857 1176 294 147 66 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 353 1767 2412 3048 3270 3720 5509 5690 5853
Size: 1414 645 636 222 450 1789 181 163
Cuts at: 5853 353
Size: 2059
Fragments arranged by size:
2059 1789 1414 645 636 450 222 181
163
BtsI GCAGTG_nn'
Cuts at: 149 376 1021 3414 4802 149
Size: 227 645 2393 1388 2906
Fragments arranged by size:
2906 2393 1388 645 227
DraI TTT'AAA
Cuts at: 4558 4558
Size: 7559
DraIII CAC_nnn'GTG
Cuts at: 428 1455 428
Size: 1027 6532
DrdI GACnn_nn'nnGTC
Cuts at: 588 5265 6123 588
Size: 4677 858 2024
Fragments arranged by size:
4677 2024 858
EaeI y'GGCC_r
Cuts at: 185 716 1594 1598 1775 2011 2066 2111 2366
Size: 531 878 4 177 236 55 45 255
Cuts at: 2366 2750 4022 4220 4263 5144 5318 5709 5736
Size: 384 1272 198 43 881 174 391 27
Cuts at: 5736 7081 185
Size: 1345 663
Fragments arranged by size:
1345 1272 881 878 663 531 391 384
255 236 198 177 174 55 45 43
27 4
EagI C'GGCC_G
Cuts at: 4220 5144 4220
Size: 924 6635
EarI CTCTTCn'nnn_
Cuts at: 1564 2338 2839 2857 3337 3373 4079 5582 5792
Size: 774 501 18 480 36 706 1503 210
Cuts at: 5792 1564
Size: 3331
Fragments arranged by size:
3331 1503 774 706 501 480 210 36
18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 929 1417 5767 6077 6223 7369 929
Size: 488 4350 310 146 1146 1119
Fragments arranged by size:
4350 1146 1119 488 310 146
EcoICRI GAG'CTC
Cuts at: 1557 4571 1557
Size: 3014 4545
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 589 1327 1475 2044 2110 2188 2521 2974
Size: 78 738 148 569 66 78 333 453
Cuts at: 2974 3159 3228 4165 5384 5816 6563 6831 511
Size: 185 69 937 1219 432 747 268 1239
Fragments arranged by size:
1239 1219 937 747 738 569 453 432
333 268 185 148 78 78 69 66
EcoNI CCTnn'n_nnAGG
Cuts at: 559 2092 4064 559
Size: 1533 1972 4054
Fragments arranged by size:
4054 1972 1533
EcoO109I rG'GnC_Cy
Cuts at: 476 551 621 1095 1477 1628 1701 1702 1893
Size: 75 70 474 382 151 73 1 191
Cuts at: 1893 3557 3918 3919 4029 4030 4140 4336 4347
Size: 1664 361 1 110 1 110 196 11
Cuts at: 4347 4507 4698 476
Size: 160 191 3337
Fragments arranged by size:
3337 1664 474 382 361 196 191 191
160 151 110 110 75 73 70 11
1 1 1
EcoRI G'AATT_C
Cuts at: 3569 4563 3569
Size: 994 6565
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2532 3831 5578 2532
Size: 1299 1747 4513
Fragments arranged by size:
4513 1747 1299
FseI GG_CCGG'CC
Cuts at: 1600 1600
Size: 7559
FspI TGC'GCA
Cuts at: 5340 5340
Size: 7559
HaeII r_GCGC'y
Cuts at: 1065 2102 2402 3277 4235 4993 5241 6263 7242
Size: 1037 300 875 958 758 248 1022 979
Cuts at: 7242 1065
Size: 1382
Fragments arranged by size:
1382 1037 1022 979 958 875 758 300
248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 586 618 1048 1080 1822 1854 1900 1932 4294
Size: 32 430 32 742 32 46 32 2362
Cuts at: 4294 4326 5401 5431 5433 5463 586
Size: 32 1075 30 2 30 2682
Fragments arranged by size:
2682 2362 1075 742 430 46 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1242 1737 2576 3177 4598 1242
Size: 495 839 601 1421 4203
Fragments arranged by size:
4203 1421 839 601 495
KasI G'GCGC_C
Cuts at: 2098 2398 4231 4989 5237 2098
Size: 300 1833 758 248 4420
Fragments arranged by size:
4420 1833 758 300 248
KpnI G_GTAC'C
Cuts at: 3665 4579 3665
Size: 914 6645
MluI A'CGCG_T
Cuts at: 6992 6992
Size: 7559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1097 2627 4187 6230 6414 1097
Size: 1530 1560 2043 184 2242
Fragments arranged by size:
2242 2043 1560 1530 184
MscI TGG'CCA
Cuts at: 2752 5320 2752
Size: 2568 4991
MslI CAynn'nnrTG
Cuts at: 295 408 649 1618 2519 2719 5548 5675 295
Size: 113 241 969 901 200 2829 127 2179
Fragments arranged by size:
2829 2179 969 901 241 200 127 113
MspA1I CmG'CkG
Cuts at: 627 908 1008 1266 1473 1863 1887 2253 4286
Size: 281 100 258 207 390 24 366 2033
Cuts at: 4286 4533 4670 4984 5344 6357 6602 627
Size: 247 137 314 360 1013 245 1584
Fragments arranged by size:
2033 1584 1013 390 366 360 314 281
258 247 245 207 137 100 24
NaeI GCC'GGC
Cuts at: 444 1598 1923 2370 2907 4243 5740 444
Size: 1154 325 447 537 1336 1497 2263
Fragments arranged by size:
2263 1497 1336 1154 537 447 325
NarI GG'CG_CC
Cuts at: 2099 2399 4232 4990 5238 2099
Size: 300 1833 758 248 4420
Fragments arranged by size:
4420 1833 758 300 248
NcoI C'CATG_G
Cuts at: 125 3665 125
Size: 3540 4019
NgoMIV G'CCGG_C
Cuts at: 442 1596 1921 2368 2905 4241 5738 442
Size: 1154 325 447 537 1336 1497 2263
Fragments arranged by size:
2263 1497 1336 1154 537 447 325
NruI TCG'CGA
Cuts at: 1256 1256
Size: 7559
NspI r_CATG'y
Cuts at: 1617 4612 5112 6019 1617
Size: 2995 500 907 3157
Fragments arranged by size:
3157 2995 907 500
PasI CC'CwG_GG
Cuts at: 547 1730 2392 3787 547
Size: 1183 662 1395 4319
Fragments arranged by size:
4319 1395 1183 662
PciI A'CATG_T
Cuts at: 6015 6015
Size: 7559
PflMI CCAn_nnn'nTGG
Cuts at: 432 432
Size: 7559
PfoI T'CCnGG_A
Cuts at: 1874 2029 2087 2165 1874
Size: 155 58 78 7268
Fragments arranged by size:
7268 155 78 58
PmeI GTTT'AAAC
Cuts at: 4558 4558
Size: 7559
PmlI CAC'GTG
Cuts at: 1040 1545 2621 1040
Size: 505 1076 5978
Fragments arranged by size:
5978 1076 505
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2197 2229 6698 6730 2197
Size: 32 4469 32 3026
Fragments arranged by size:
4469 3026 32 32
PpuMI rG'GwC_Cy
Cuts at: 476 551 621 1095 1477 3557 476
Size: 75 70 474 382 2080 4478
Fragments arranged by size:
4478 2080 474 382 75 70
PshAI GACnn'nnGTC
Cuts at: 467 467
Size: 7559
PspOMI G'GGCC_C
Cuts at: 1701 3918 4029 4096 4169 4336 4507 1701
Size: 2217 111 67 73 167 171 4753
Fragments arranged by size:
4753 2217 171 167 111 73 67
PspXI vC'TCGA_Gb
Cuts at: 1646 1646
Size: 7559
PstI C_TGCA'G
Cuts at: 560 632 1892 2632 2792 3701 4439 4606 5291
Size: 72 1260 740 160 909 738 167 685
Cuts at: 5291 560
Size: 2828
Fragments arranged by size:
2828 1260 909 740 738 685 167 160
72
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7559
PvuII CAG'CTG
Cuts at: 1008 1266 1473 1863 1887 2253 4533 4984 5344
Size: 258 207 390 24 366 2280 451 360
Cuts at: 5344 1008
Size: 3223
Fragments arranged by size:
3223 2280 451 390 366 360 258 207
24
RsrII CG'GwC_CG
Cuts at: 5754 5754
Size: 7559
SacI G_AGCT'C
Cuts at: 1559 4573 1559
Size: 3014 4545
SalI G'TCGA_C
Cuts at: 2574 4596 2574
Size: 2022 5537
SanDI GG'GwC_CC
Cuts at: 551 3557 551
Size: 3006 4553
SapI GCTCTTCn'nnn_
Cuts at: 1564 2857 5582 5792 1564
Size: 1293 2725 210 3331
Fragments arranged by size:
3331 2725 1293 210
SbfI CC_TGCA'GG
Cuts at: 4439 4606 4439
Size: 167 7392
ScaI AGT'ACT
Cuts at: 3019 7076 3019
Size: 4057 3502
SexAI A'CCwGG_T
Cuts at: 1739 3179 4448 1739
Size: 1440 1269 4850
Fragments arranged by size:
4850 1440 1269
SfcI C'TryA_G
Cuts at: 33 556 628 1299 1888 2628 2788 3697 4435
Size: 523 72 671 589 740 160 909 738
Cuts at: 4435 4602 5287 6280 6471 33
Size: 167 685 993 191 1121
Fragments arranged by size:
1121 993 909 740 738 685 671 589
523 191 167 160 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 2387 2387
Size: 7559
SfoI GGC'GCC
Cuts at: 2100 2400 4233 4991 5239 2100
Size: 300 1833 758 248 4420
Fragments arranged by size:
4420 1833 758 300 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7559
SmaI CCC'GGG
Cuts at: 1699 1810 2384 3495 3909 4101 4168 4581 1699
Size: 111 574 1111 414 192 67 413 4677
Fragments arranged by size:
4677 1111 574 414 413 192 111 67
SmlI C'TyrA_G
Cuts at: 1646 3232 3514 4495 4714 5629 6121 6383 1646
Size: 1586 282 981 219 915 492 262 2822
Fragments arranged by size:
2822 1586 981 915 492 282 262 219
SnaBI TAC'GTA
Cuts at: 4551 4551
Size: 7559
SpeI A'CTAG_T
Cuts at: 67 2979 67
Size: 2912 4647
SphI G_CATG'C
Cuts at: 1617 4612 5112 1617
Size: 2995 500 4064
Fragments arranged by size:
4064 2995 500
StuI AGG'CCT
Cuts at: 197 3106 4493 197
Size: 2909 1387 3263
Fragments arranged by size:
3263 2909 1387
StyI C'CwwG_G
Cuts at: 125 192 1637 1679 2150 2580 3665 3815 4025
Size: 67 1445 42 471 430 1085 150 210
Cuts at: 4025 4693 5047 125
Size: 668 354 2637
Fragments arranged by size:
2637 1445 1085 668 471 430 354 210
150 67 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 205 439 937 1447 1466 1618 2697 2709 2809
Size: 234 498 510 19 152 1079 12 100
Cuts at: 2809 3019 5142 5275 5734 5943 205
Size: 210 2123 133 459 209 1821
Fragments arranged by size:
2123 1821 1079 510 498 459 234 210
209 152 133 100 19 12
TatI w'GTAC_w
Cuts at: 3017 3446 7074 3017
Size: 429 3628 3502
Fragments arranged by size:
3628 3502 429
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 144 176 229 261 7076 7108 144
Size: 32 53 32 6815 32 595
Fragments arranged by size:
6815 595 53 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 508 2119 2476 3609 5356 508
Size: 1611 357 1133 1747 2711
Fragments arranged by size:
2711 1747 1611 1133 357
XbaI T'CTAG_A
Cuts at: 4590 4590
Size: 7559
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1080 1452 1470 2157 2361 3813 5054 1080
Size: 372 18 687 204 1452 1241 3585
Fragments arranged by size:
3585 1452 1241 687 372 204 18
XhoI C'TCGA_G
Cuts at: 1646 1646
Size: 7559
XmaI C'CCGG_G
Cuts at: 1697 1808 2382 3493 3907 4099 4166 4579 1697
Size: 111 574 1111 414 192 67 413 4677
Fragments arranged by size:
4677 1111 574 414 413 192 111 67
XmnI GAAnn'nnTTC
Cuts at: 535 2515 535
Size: 1980 5579
ZraI GAC'GTC
Cuts at: 288 1833 2085 288
Size: 1545 252 5762
Fragments arranged by size:
5762 1545 252
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AscI AvrII BfrBI BmgBI BmtI BsiWI
BsrGI BssHII BstZ17I ClaI CspCI EcoRV FspAI HindIII
HpaI MfeI NdeI NheI NotI NsiI PacI PsiI
PsrI SacII SgrAI SrfI SspI SwaI