Restriction map for
pF1KSDA0817
(Circular) (Six-base) MAPSORT of: pF1KSDA0817.seq Check: 4039 from: 1 to: 11471
pF1KSDA0817 11471 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 219 682 1104 1248 1791 2545 2552 2854 4198
Size: 463 422 144 543 754 7 302 1344
Cuts at: 4198 219
Size: 7492
Fragments arranged by size:
7492 1344 754 543 463 422 302 144
7
AatII G_ACGT'C
Cuts at: 5706 5706
Size: 11471
AccI GT'mk_AC
Cuts at: 4958 5700 8509 4958
Size: 742 2809 7920
Fragments arranged by size:
7920 2809 742
Acc65I G'GTAC_C
Cuts at: 2876 8487 2876
Size: 5611 5860
AclI AA'CG_TT
Cuts at: 11311 11311
Size: 11471
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 477 1038 1435 1453 1962 2764 5281 5594 6018
Size: 561 397 18 509 802 2517 313 424
Cuts at: 6018 9296 9728 10475 10743 477
Size: 3278 432 747 268 1205
Fragments arranged by size:
3278 2517 1205 802 747 561 509 432
424 397 313 268 18
AflIII A'CryG_T
Cuts at: 1043 1671 3737 4985 6412 7087 8823 9927 10904
Size: 628 2066 1248 1427 675 1736 1104 977
Cuts at: 10904 1043
Size: 1610
Fragments arranged by size:
2066 1736 1610 1427 1248 1104 977 675
628
AgeI A'CCGG_T
Cuts at: 9859 9859
Size: 11471
AhdI GACnn_n'nnGTC
Cuts at: 3884 6950 7112 7290 3884
Size: 3066 162 178 8065
Fragments arranged by size:
8065 3066 178 162
AleI CACnn'nnGTG
Cuts at: 2984 3736 4468 4900 5314 7381 7456 2984
Size: 752 732 432 414 2067 75 6999
Fragments arranged by size:
6999 2067 752 732 432 414 75
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 496 5910 6205 6318 496
Size: 5414 295 113 5649
Fragments arranged by size:
5649 5414 295 113
AlwNI CAG_nnn'CTG
Cuts at: 234 1609 1835 2095 2368 2784 3434 4062 4320
Size: 1375 226 260 273 416 650 628 258
Cuts at: 4320 4527 4581 4869 4872 6201 6767 7830 7950
Size: 207 54 288 3 1329 566 1063 120
Cuts at: 7950 8776 10343 234
Size: 826 1567 1362
Fragments arranged by size:
1567 1375 1362 1329 1063 826 650 628
566 416 288 273 260 258 226 207
120 54 3
ApaI G_GGCC'C
Cuts at: 201 1118 1459 3053 3451 4159 4357 4606 5432
Size: 917 341 1594 398 708 198 249 826
Cuts at: 5432 7553 7799 8153 8225 8297 201
Size: 2121 246 354 72 72 3375
Fragments arranged by size:
3375 2121 1594 917 826 708 398 354
341 249 246 198 72 72
ApaLI G'TGCA_C
Cuts at: 366 1525 2029 5934 7053 10241 366
Size: 1159 504 3905 1119 3188 1596
Fragments arranged by size:
3905 3188 1596 1159 1119 504
ApoI r'AATT_y
Cuts at: 7372 8475 10824 11080 7372
Size: 1103 2349 256 7763
Fragments arranged by size:
7763 2349 1103 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 11471
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 11471
AvaI C'yCGr_G
Cuts at: 200 552 1110 1841 3378 3404 3683 3874 4159
Size: 352 558 731 1537 26 279 191 285
Cuts at: 4159 5032 5132 7553 8233 8491 200
Size: 873 100 2421 680 258 3180
Fragments arranged by size:
3180 2421 1537 873 731 680 558 352
285 279 258 191 100 26
AvrII C'CTAG_G
Cuts at: 2050 3339 2050
Size: 1289 10182
BamHI G'GATC_C
Cuts at: 332 3678 7566 8496 332
Size: 3346 3888 930 3307
Fragments arranged by size:
3888 3346 3307 930
BanI G'GyrC_C
Cuts at: 243 670 823 1089 1758 1779 1862 2011 2519
Size: 427 153 266 669 21 83 149 508
Cuts at: 2519 2604 2737 2828 2876 2930 3026 3176 3640
Size: 85 133 91 48 54 96 150 464
Cuts at: 3640 4660 4894 5606 6320 6479 7600 7801 8121
Size: 1020 234 712 714 159 1121 201 320
Cuts at: 8121 8311 8487 8901 9149 9184 243
Size: 190 176 414 248 35 2530
Fragments arranged by size:
2530 1121 1020 714 712 669 508 464
427 414 320 266 248 234 201 190
176 159 153 150 149 133 96 91
85 83 54 48 35 21
BanII G_rGCy'C
Cuts at: 201 1085 1118 1459 2450 2562 3053 3451 3614
Size: 884 33 341 991 112 491 398 163
Cuts at: 3614 3760 4135 4159 4220 4357 4606 4914 5039
Size: 146 375 24 61 137 249 308 125
Cuts at: 5039 5270 5432 5662 6208 6629 7319 7553 7799
Size: 231 162 230 546 421 690 234 246
Cuts at: 7799 8084 8114 8153 8225 8240 8297 8485 9515
Size: 285 30 39 72 15 57 188 1030
Cuts at: 9515 201
Size: 2157
Fragments arranged by size:
2157 1030 991 884 690 546 491 421
398 375 341 308 285 249 246 234
231 230 188 163 162 146 137 125
112 72 61 57 39 33 30 24
15
BbeI G_GCGC'C
Cuts at: 247 827 6324 7604 7805 8315 8905 9153 247
Size: 580 5497 1280 201 510 590 248 2565
Fragments arranged by size:
5497 2565 1280 590 580 510 248 201
BbsI GAAGACnn'nnnn_
Cuts at: 2115 5871 6968 7866 7878 2115
Size: 3756 1097 898 12 5708
Fragments arranged by size:
5708 3756 1097 898 12
BbvCI CC'TCA_GC
Cuts at: 3211 5292 3211
Size: 2081 9390
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 7720 7754 7720
Size: 34 11437
BciVI GTATCCnnnnn_n'
Cuts at: 9369 10130 11471 9369
Size: 761 1341 9369
Fragments arranged by size:
9369 1341 761
BclI T'GATC_A
Cuts at: 8991 8991
Size: 11471
BglI GCCn_nnn'nGGC
Cuts at: 557 1972 2723 2869 3124 3236 3512 4524 6436
Size: 1415 751 146 255 112 276 1012 1912
Cuts at: 6436 6650 7028 7945 8079 8292 557
Size: 214 378 917 134 213 3736
Fragments arranged by size:
3736 1912 1415 1012 917 751 378 276
255 214 213 146 134 112
BglII A'GATC_T
Cuts at: 2084 2426 8986 11023 2084
Size: 342 6560 2037 2532
Fragments arranged by size:
6560 2532 2037 342
BlpI GC'TnA_GC
Cuts at: 648 1610 3937 7812 8583 648
Size: 962 2327 3875 771 3536
Fragments arranged by size:
3875 3536 2327 962 771
Bme1580I G_kGCm'C
Cuts at: 201 370 553 1118 1332 1459 1529 1763 2033
Size: 169 183 565 214 127 70 234 270
Cuts at: 2033 2375 2742 3053 3451 3572 3645 4159 4193
Size: 342 367 311 398 121 73 514 34
Cuts at: 4193 4357 4606 4649 5432 5938 6482 7057 7144
Size: 164 249 43 783 506 544 575 87
Cuts at: 7144 7553 7799 8153 8225 8297 9096 9189 10245
Size: 409 246 354 72 72 799 93 1056
Cuts at: 10245 201
Size: 1427
Fragments arranged by size:
1427 1056 799 783 575 565 544 514
506 409 398 367 354 342 311 270
249 246 234 214 183 169 164 127
121 93 87 73 72 72 70 43
34
BmgBI CAC'GTC
Cuts at: 724 1044 2132 3147 6029 7292 7407 724
Size: 320 1088 1015 2882 1263 115 4788
Fragments arranged by size:
4788 2882 1263 1088 1015 320 115
BmrI ACTGGGnnnn_n'
Cuts at: 1072 1086 1649 3028 3763 4095 4304 6948 7157
Size: 14 563 1379 735 332 209 2644 209
Cuts at: 7157 8683 8764 9099 11243 1072
Size: 1526 81 335 2144 1300
Fragments arranged by size:
2644 2144 1526 1379 1300 735 563 335
332 209 209 81 14
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2103 5058 5265 6291 9838 2103
Size: 2955 207 1026 3547 3736
Fragments arranged by size:
3736 3547 2955 1026 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1086 1196 2158 2464 3770 4712 4894 5088 5260
Size: 110 962 306 1306 942 182 194 172
Cuts at: 5260 5777 6852 8263 1086
Size: 517 1075 1411 4294
Fragments arranged by size:
4294 1411 1306 1075 962 942 517 306
194 182 172 110
Bpu10I CC'TnA_GC
Cuts at: 2057 3211 5292 6290 2057
Size: 1154 2081 998 7238
Fragments arranged by size:
7238 2081 1154 998
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 7729 8647 9526 10018 10316 7729
Size: 918 879 492 298 8884
Fragments arranged by size:
8884 918 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4393 4942 5980 7778 11175 4393
Size: 549 1038 1798 3397 4689
Fragments arranged by size:
4689 3397 1798 1038 549
BsaAI yAC'GTr
Cuts at: 371 1407 3275 4986 7656 8463 8824 371
Size: 1036 1868 1711 2670 807 361 3018
Fragments arranged by size:
3018 2670 1868 1711 1036 807 361
BsaBI GATnn'nnATC
Cuts at: 6 8985 11022 6
Size: 8979 2037 455
Fragments arranged by size:
8979 2037 455
BsaHI Gr'CG_yC
Cuts at: 244 824 4697 5703 5770 6321 6420 7484 7601
Size: 580 3873 1006 67 551 99 1064 117
Cuts at: 7601 7802 8312 8902 9150 9852 11348 244
Size: 201 510 590 248 702 1496 367
Fragments arranged by size:
3873 1496 1064 1006 702 590 580 551
510 367 248 201 117 99 67
BsaWI w'CCGG_w
Cuts at: 329 5708 8660 9181 9859 10133 10280 10691 329
Size: 5379 2952 521 678 274 147 411 1109
Fragments arranged by size:
5379 2952 1109 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 385 415 432 462 895 925 1186 1216 1342
Size: 30 17 30 433 30 261 30 126
Cuts at: 1342 1372 1534 1564 1795 1825 1980 2010 2914
Size: 30 162 30 231 30 155 30 904
Cuts at: 2914 2944 5935 5965 6112 6142 7258 7288 8261
Size: 30 2991 30 147 30 1116 30 973
Cuts at: 8261 8291 385
Size: 30 3565
Fragments arranged by size:
3565 2991 1116 973 904 433 261 231
162 155 147 126 30 30 30 30
30 30 30 30 30 30 30 30
30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1098 1194 2239 2644 2770 3718 3868 4092 5455
Size: 96 1045 405 126 948 150 224 1363
Cuts at: 5455 6940 7589 7918 1098
Size: 1485 649 329 4651
Fragments arranged by size:
4651 1485 1363 1045 948 649 405 329
224 150 126 96
BseYI C'CCAG_C
Cuts at: 177 323 516 614 760 1711 2210 2237 2900
Size: 146 193 98 146 951 499 27 663
Cuts at: 2900 3122 4238 4530 4674 5646 7230 7512 7561
Size: 222 1116 292 144 972 1584 282 49
Cuts at: 7561 7605 7773 8077 8206 8218 8383 8896 9654
Size: 44 168 304 129 12 165 513 758
Cuts at: 9654 10231 10727 177
Size: 577 496 921
Fragments arranged by size:
1584 1116 972 951 921 758 663 577
513 499 496 304 292 282 222 193
168 165 146 146 144 129 98 49
44 27 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 721 1868 2158 2451 2637 3324 3915 4066
Size: 252 1147 290 293 186 687 591 151
Cuts at: 4066 4432 5032 5583 5823 6028 6555 7384 8338
Size: 366 600 551 240 205 527 829 954
Cuts at: 8338 469
Size: 3602
Fragments arranged by size:
3602 1147 954 829 687 600 591 551
527 366 293 290 252 240 205 186
151
BsiEI CG_ry'CG
Cuts at: 104 1728 7344 9013 9059 10267 104
Size: 1624 5616 1669 46 1208 1308
Fragments arranged by size:
5616 1669 1624 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 370 442 649 1085 1529 2033 2689 3494 3873
Size: 72 207 436 444 504 656 805 379
Cuts at: 3873 5039 5938 6291 7057 7736 7928 8485 9263
Size: 1166 899 353 766 679 192 557 778
Cuts at: 9263 9453 10245 370
Size: 190 792 1596
Fragments arranged by size:
1596 1166 899 805 792 778 766 679
656 557 504 444 436 379 353 207
192 190 72
BsmI GAATG_Cn'
Cuts at: 1858 4189 6685 6709 1858
Size: 2331 2496 24 6620
Fragments arranged by size:
6620 2496 2331 24
BsmBI CGTCTCn'nnnn_
Cuts at: 2254 5710 6490 7129 2254
Size: 3456 780 639 6596
Fragments arranged by size:
6596 3456 780 639
Bsp1286I G_dGCh'C
Cuts at: 201 370 442 553 649 1085 1118 1332 1459
Size: 169 72 111 96 436 33 214 127
Cuts at: 1459 1529 1763 2033 2375 2450 2562 2689 2742
Size: 70 234 270 342 75 112 127 53
Cuts at: 2742 3053 3451 3494 3572 3614 3645 3760 3873
Size: 311 398 43 78 42 31 115 113
Cuts at: 3873 4135 4159 4193 4220 4357 4606 4649 4914
Size: 262 24 34 27 137 249 43 265
Cuts at: 4914 5039 5270 5432 5662 5938 6208 6291 6482
Size: 125 231 162 230 276 270 83 191
Cuts at: 6482 6629 7057 7144 7319 7553 7736 7799 7928
Size: 147 428 87 175 234 183 63 129
Cuts at: 7928 8084 8114 8153 8225 8240 8297 8485 9096
Size: 156 30 39 72 15 57 188 611
Cuts at: 9096 9189 9263 9453 9515 10245 201
Size: 93 74 190 62 730 1427
Fragments arranged by size:
1427 730 611 436 428 398 342 311
276 270 270 265 262 249 234 234
231 230 214 191 190 188 183 175
169 162 156 147 137 129 127 127
125 115 113 112 111 96 93 87
83 78 75 74 72 72 70 63
62 57 53 43 43 42 39 34
33 31 30 27 24 15
BspEI T'CCGG_A
Cuts at: 5708 10691 5708
Size: 4983 6488
BspHI T'CATG_A
Cuts at: 10647 10647
Size: 11471
BspMI ACCTGCnnnn'nnnn_
Cuts at: 219 521 682 743 1104 1120 1248 1791 2545
Size: 302 161 61 361 16 128 543 754
Cuts at: 2545 2552 2854 2965 3202 3334 3365 4198 5722
Size: 7 302 111 237 132 31 833 1524
Cuts at: 5722 6224 6826 7772 8521 8735 9037 9418 219
Size: 502 602 946 749 214 302 381 2272
Fragments arranged by size:
2272 1524 946 833 754 749 602 543
502 381 361 302 302 302 237 214
161 132 128 111 61 31 16 7
BsrBI CCG'CTC
Cuts at: 50 2456 2769 4563 4622 5414 8105 9763 9817
Size: 2406 313 1794 59 792 2691 1658 54
Cuts at: 9817 11301 11466 50
Size: 1484 165 55
Fragments arranged by size:
2691 2406 1794 1658 1484 792 313 165
59 55 54
BsrDI GCAATG_nn'
Cuts at: 1527 2562 3006 4290 4744 5391 5835 6327 9383
Size: 1035 444 1284 454 647 444 492 3056
Cuts at: 9383 1527
Size: 3615
Fragments arranged by size:
3615 3056 1284 1035 647 492 454 444
444
BsrFI r'CCGG_y
Cuts at: 988 1352 1727 2717 3506 3548 3688 4891 6066
Size: 364 375 990 789 42 140 1203 1175
Cuts at: 6066 6547 6821 7706 7973 8073 8143 9469 9650
Size: 481 274 885 267 100 70 1326 181
Cuts at: 9650 9859 988
Size: 209 2600
Fragments arranged by size:
2600 1326 1203 1175 990 885 789 481
375 364 274 267 209 181 140 100
70 42
BsrGI T'GTAC_A
Cuts at: 1817 2913 4541 7494 1817
Size: 1096 1628 2953 5794
Fragments arranged by size:
5794 2953 1628 1096
BssSI C'ACGA_G
Cuts at: 2980 4075 4963 7729 8356 9742 10100 2980
Size: 1095 888 2766 627 1386 358 4351
Fragments arranged by size:
4351 2766 1386 1095 888 627 358
BstAPI GCAn_nnn'nTGC
Cuts at: 234 1096 1862 3929 5606 5723 7061 7830 8352
Size: 862 766 2067 1677 117 1338 769 522
Cuts at: 8352 8848 234
Size: 496 2857
Fragments arranged by size:
2857 2067 1677 1338 862 769 766 522
496 117
BstBI TT'CG_AA
Cuts at: 110 9832 110
Size: 9722 1749
BstEII G'GTnAC_C
Cuts at: 889 8022 889
Size: 7133 4338
BstXI CCAn_nnnn'nTGG
Cuts at: 132 156 760 1491 1932 2353 2512 2986 3250
Size: 24 604 731 441 421 159 474 264
Cuts at: 3250 4374 5152 6069 7072 8287 132
Size: 1124 778 917 1003 1215 3316
Fragments arranged by size:
3316 1215 1124 1003 917 778 731 604
474 441 421 264 159 24
BstYI r'GATC_y
Cuts at: 1 332 2084 2336 2426 3678 7566 8496 8963
Size: 331 1752 252 90 1252 3888 930 467
Cuts at: 8963 8986 9321 9567 10579 10665 10677 11023 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
3888 1752 1252 1012 930 467 449 346
335 331 252 246 90 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 468 1634 4232 6458 468
Size: 1166 2598 2226 5481
Fragments arranged by size:
5481 2598 2226 1166
BtgI C'CryG_G
Cuts at: 125 149 570 666 977 1304 1341 1734 1999
Size: 24 421 96 311 327 37 393 265
Cuts at: 1999 2926 3104 3184 3294 3585 4466 4656 4766
Size: 927 178 80 110 291 881 190 110
Cuts at: 4766 5312 5610 6522 6557 6674 7421 7615 7636
Size: 546 298 912 35 117 747 194 21
Cuts at: 7636 7692 8162 125
Size: 56 470 3434
Fragments arranged by size:
3434 927 912 881 747 546 470 421
393 327 311 298 291 265 194 190
178 117 110 110 96 80 56 37
35 24 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2957 4598 6240 9421 9602 9765 2957
Size: 1641 1642 3181 181 163 4663
Fragments arranged by size:
4663 3181 1642 1641 181 163
BtsI GCAGTG_nn'
Cuts at: 583 1361 3225 3261 3312 3432 3492 3591 3645
Size: 778 1864 36 51 120 60 99 54
Cuts at: 3645 4251 4430 5724 5938 6085 6202 6633 6729
Size: 606 179 1294 214 147 117 431 96
Cuts at: 6729 6781 7249 7596 7685 8201 8269 8338 8397
Size: 52 468 347 89 516 68 69 59
Cuts at: 8397 8714 583
Size: 317 3340
Fragments arranged by size:
3340 1864 1294 778 606 516 468 431
347 317 214 179 147 120 117 99
96 89 69 68 60 59 54 52
51 36
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3588 3623 6820 6855 3588
Size: 35 3197 35 8204
Fragments arranged by size:
8204 3197 35 35
DraI TTT'AAA
Cuts at: 8470 8470
Size: 11471
DraIII CAC_nnn'GTG
Cuts at: 365 592 777 3586 4493 5606 7921 365
Size: 227 185 2809 907 1113 2315 3915
Fragments arranged by size:
3915 2809 2315 1113 907 227 185
DrdI GACnn_nn'nnGTC
Cuts at: 1831 7432 7483 7671 7711 7870 9177 10035 1831
Size: 5601 51 188 40 159 1307 858 3267
Fragments arranged by size:
5601 3267 1307 858 188 159 51 40
EaeI y'GGCC_r
Cuts at: 146 170 974 1296 1725 2804 3291 3588 3845
Size: 24 804 322 429 1079 487 297 257
Cuts at: 3845 4026 4653 6233 6366 7074 7236 7341 7633
Size: 181 627 1580 133 708 162 105 292
Cuts at: 7633 8000 8264 9056 9230 9621 9648 10993 146
Size: 367 264 792 174 391 27 1345 624
Fragments arranged by size:
1580 1345 1079 804 792 708 627 624
487 429 391 367 322 297 292 264
257 181 174 162 133 105 27 24
EagI C'GGCC_G
Cuts at: 1725 7341 9056 1725
Size: 5616 1715 4140
Fragments arranged by size:
5616 4140 1715
EarI CTCTTCn'nnn_
Cuts at: 496 1204 1219 2302 2740 7840 7912 9494 9704
Size: 708 15 1083 438 5100 72 1582 210
Cuts at: 9704 496
Size: 2263
Fragments arranged by size:
5100 2263 1582 1083 708 438 210 72
15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 440 903 1213 1289 2570 3374 7381 8052 8089
Size: 463 310 76 1281 804 4007 671 37
Cuts at: 8089 9679 9989 10135 11281 440
Size: 1590 310 146 1146 630
Fragments arranged by size:
4007 1590 1281 1146 804 671 630 463
310 310 146 76 37
EcoICRI GAG'CTC
Cuts at: 1083 5037 8483 1083
Size: 3954 3446 4071
Fragments arranged by size:
4071 3954 3446
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 477 1038 1086 1196 1435 1453 1962 2158 2464
Size: 561 48 110 239 18 509 196 306
Cuts at: 2464 2764 3770 4712 4894 5088 5260 5281 5594
Size: 300 1006 942 182 194 172 21 313
Cuts at: 5594 5777 6018 6852 8263 9296 9728 10475 10743
Size: 183 241 834 1411 1033 432 747 268
Cuts at: 10743 477
Size: 1205
Fragments arranged by size:
1411 1205 1033 1006 942 834 747 561
509 432 313 306 300 268 241 239
196 194 183 182 172 110 48 21
18
EcoNI CCTnn'n_nnAGG
Cuts at: 136 2548 4519 7933 136
Size: 2412 1971 3414 3674
Fragments arranged by size:
3674 3414 2412 1971
EcoO109I rG'GnC_Cy
Cuts at: 197 312 622 789 870 936 1114 1115 1455
Size: 115 310 167 81 66 178 1 340
Cuts at: 1455 1845 1898 2005 2110 2365 2588 2661 3049
Size: 390 53 107 105 255 223 73 388
Cuts at: 3049 3050 3090 3447 3448 3576 3878 4043 4059
Size: 1 40 357 1 128 302 165 16
Cuts at: 4059 4155 4156 4353 4354 4506 4602 4716 4755
Size: 96 1 197 1 152 96 114 39
Cuts at: 4755 5428 5429 5594 5615 5834 6262 6428 6815
Size: 673 1 165 21 219 428 166 387
Cuts at: 6815 6952 7336 7550 7641 7827 8150 8158 8294
Size: 137 384 214 91 186 323 8 136
Cuts at: 8294 8610 197
Size: 316 3058
Fragments arranged by size:
3058 673 428 390 388 387 384 357
340 323 316 310 302 255 223 219
214 197 186 178 167 166 165 165
152 137 136 128 115 114 107 105
96 96 91 81 73 66 53 40
39 21 16 8 1 1 1 1
1 1
EcoRI G'AATT_C
Cuts at: 8475 8475
Size: 11471
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 9490 9490
Size: 11471
FseI GG_CCGG'CC
Cuts at: 1731 1731
Size: 11471
FspI TGC'GCA
Cuts at: 9252 9252
Size: 11471
HaeII r_GCGC'y
Cuts at: 247 827 1651 2238 2280 5334 6324 7333 7604
Size: 580 824 587 42 3054 990 1009 271
Cuts at: 7604 7805 7974 8315 8905 9153 10175 11154 247
Size: 201 169 341 590 248 1022 979 564
Fragments arranged by size:
3054 1022 1009 990 979 824 590 587
580 564 341 271 248 201 169 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 385 417 1186 1218 1795 1827 3241 3273 3870
Size: 32 769 32 577 32 1414 32 597
Cuts at: 3870 3902 6936 6968 7098 7130 7276 7308 9313
Size: 32 3034 32 130 32 146 32 2005
Cuts at: 9313 9343 9345 9375 385
Size: 30 2 30 2481
Fragments arranged by size:
3034 2481 2005 1414 769 597 577 146
130 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 5379 5701 8510 5379
Size: 322 2809 8340
Fragments arranged by size:
8340 2809 322
HindIII A'AGCT_T
Cuts at: 461 5576 461
Size: 5115 6356
KasI G'GCGC_C
Cuts at: 243 823 6320 7600 7801 8311 8901 9149 243
Size: 580 5497 1280 201 510 590 248 2565
Fragments arranged by size:
5497 2565 1280 590 580 510 248 201
KpnI G_GTAC'C
Cuts at: 2880 8491 2880
Size: 5611 5860
MluI A'CGCG_T
Cuts at: 10904 10904
Size: 11471
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3977 5839 6786 10142 10326 3977
Size: 1862 947 3356 184 5122
Fragments arranged by size:
5122 3356 1862 947 184
MscI TGG'CCA
Cuts at: 148 1298 2806 3293 3590 7076 8002 8266 9232
Size: 1150 1508 487 297 3486 926 264 966
Cuts at: 9232 148
Size: 2387
Fragments arranged by size:
3486 2387 1508 1150 966 926 487 297
264
MslI CAynn'nnrTG
Cuts at: 968 2324 2984 3736 4468 4900 5314 6067 6527
Size: 1356 660 752 732 432 414 753 460
Cuts at: 6527 7261 7381 7456 8449 9460 9587 968
Size: 734 120 75 993 1011 127 2852
Fragments arranged by size:
2852 1356 1011 993 753 752 734 732
660 460 432 414 127 120 75
MspA1I CmG'CkG
Cuts at: 225 417 701 820 1241 1724 1928 2069 3122
Size: 192 284 119 421 483 204 141 1053
Cuts at: 3122 3137 3186 3234 3398 4142 4389 4651 4690
Size: 15 49 48 164 744 247 262 39
Cuts at: 4690 4869 4884 5783 6371 6392 6539 6641 6686
Size: 179 15 899 588 21 147 102 45
Cuts at: 6686 6786 6802 7230 7638 7694 7959 8283 8582
Size: 100 16 428 408 56 265 324 299
Cuts at: 8582 8896 9256 10269 10514 225
Size: 314 360 1013 245 1182
Fragments arranged by size:
1182 1053 1013 899 744 588 483 428
421 408 360 324 314 299 284 265
262 247 245 204 192 179 164 147
141 119 102 100 56 49 48 45
39 21 16 15 15
NaeI GCC'GGC
Cuts at: 990 1729 2719 3508 3550 6823 7975 8075 9652
Size: 739 990 789 42 3273 1152 100 1577
Cuts at: 9652 990
Size: 2809
Fragments arranged by size:
3273 2809 1577 1152 990 789 739 100
42
NarI GG'CG_CC
Cuts at: 244 824 6321 7601 7802 8312 8902 9150 244
Size: 580 5497 1280 201 510 590 248 2565
Fragments arranged by size:
5497 2565 1280 590 580 510 248 201
NcoI C'CATG_G
Cuts at: 125 149 1304 1341 3294 4466 5312 5610 6557
Size: 24 1155 37 1953 1172 846 298 947
Cuts at: 6557 8162 125
Size: 1605 3434
Fragments arranged by size:
3434 1953 1605 1172 1155 947 846 298
37 24
NdeI CA'TA_TG
Cuts at: 4641 4641
Size: 11471
NgoMIV G'CCGG_C
Cuts at: 988 1727 2717 3506 3548 6821 7973 8073 9650
Size: 739 990 789 42 3273 1152 100 1577
Cuts at: 9650 988
Size: 2809
Fragments arranged by size:
3273 2809 1577 1152 990 789 739 100
42
NspI r_CATG'y
Cuts at: 1420 1675 2714 5958 6416 7091 7148 8454 8524
Size: 255 1039 3244 458 675 57 1306 70
Cuts at: 8524 9024 9931 1420
Size: 500 907 2960
Fragments arranged by size:
3244 2960 1306 1039 907 675 500 458
255 70 57
PasI CC'CwG_GG
Cuts at: 132 193 687 885 2016 2281 2368 4521 5514
Size: 61 494 198 1131 265 87 2153 993
Cuts at: 5514 6279 6597 8153 132
Size: 765 318 1556 3450
Fragments arranged by size:
3450 2153 1556 1131 993 765 494 318
265 198 87 61
PciI A'CATG_T
Cuts at: 1671 6412 7087 9927 1671
Size: 4741 675 2840 3215
Fragments arranged by size:
4741 3215 2840 675
PflMI CCAn_nnn'nTGG
Cuts at: 923 1305 3586 4320 4467 4493 4767 5460 5646
Size: 382 2281 734 147 26 274 693 186
Cuts at: 5646 923
Size: 6748
Fragments arranged by size:
6748 2281 734 693 382 274 186 147
26
PfoI T'CCnGG_A
Cuts at: 353 2441 3974 5180 5237 6868 7040 7569 353
Size: 2088 1533 1206 57 1631 172 529 4255
Fragments arranged by size:
4255 2088 1631 1533 1206 529 172 57
PmeI GTTT'AAAC
Cuts at: 8470 8470
Size: 11471
PmlI CAC'GTG
Cuts at: 1407 3275 1407
Size: 1868 9603
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10610 10642 10610
Size: 32 11439
PpuMI rG'GwC_Cy
Cuts at: 622 789 870 936 1845 1898 2005 2588 2661
Size: 167 81 66 909 53 107 583 73
Cuts at: 2661 3576 3878 4716 5834 6262 6815 6952 8158
Size: 915 302 838 1118 428 553 137 1206
Cuts at: 8158 622
Size: 3935
Fragments arranged by size:
3935 1206 1118 915 909 838 583 553
428 302 167 137 107 81 73 66
53
PshAI GACnn'nnGTC
Cuts at: 941 941
Size: 11471
PspOMI G'GGCC_C
Cuts at: 197 1114 1455 3049 3447 4155 4353 4602 5428
Size: 917 341 1594 398 708 198 249 826
Cuts at: 5428 7549 7795 8149 8221 8293 197
Size: 2121 246 354 72 72 3375
Fragments arranged by size:
3375 2121 1594 917 826 708 398 354
341 249 246 198 72 72
PstI C_TGCA'G
Cuts at: 641 2276 2776 2975 3421 4209 5719 6194 7028
Size: 1635 500 199 446 788 1510 475 834
Cuts at: 7028 7542 8518 9203 641
Size: 514 976 685 2909
Fragments arranged by size:
2909 1635 1510 976 834 788 685 514
500 475 446 199
PvuI CG_AT'CG
Cuts at: 104 104
Size: 11471
PvuII CAG'CTG
Cuts at: 820 1928 4389 4869 4884 6539 6802 8283 8896
Size: 1108 2461 480 15 1655 263 1481 613
Cuts at: 8896 9256 820
Size: 360 3035
Fragments arranged by size:
3035 2461 1655 1481 1108 613 480 360
263 15
RsrII CG'GwC_CG
Cuts at: 3999 9666 3999
Size: 5667 5804
SacI G_AGCT'C
Cuts at: 1085 5039 8485 1085
Size: 3954 3446 4071
Fragments arranged by size:
4071 3954 3446
SacII CC_GC'GG
Cuts at: 3187 7639 7695 3187
Size: 4452 56 6963
Fragments arranged by size:
6963 4452 56
SalI G'TCGA_C
Cuts at: 5699 8508 5699
Size: 2809 8662
SanDI GG'GwC_CC
Cuts at: 789 1845 1898 2588 3576 3878 8158 789
Size: 1056 53 690 988 302 4280 4102
Fragments arranged by size:
4280 4102 1056 988 690 302 53
SapI GCTCTTCn'nnn_
Cuts at: 7840 9494 9704 7840
Size: 1654 210 9607
Fragments arranged by size:
9607 1654 210
SbfI CC_TGCA'GG
Cuts at: 641 7028 8518 641
Size: 6387 1490 3594
Fragments arranged by size:
6387 3594 1490
ScaI AGT'ACT
Cuts at: 6910 8362 10988 6910
Size: 1452 2626 7393
Fragments arranged by size:
7393 2626 1452
SexAI A'CCwGG_T
Cuts at: 5372 5602 5372
Size: 230 11241
SfcI C'TryA_G
Cuts at: 33 276 637 2272 2772 2971 3031 3417 3859
Size: 243 361 1635 500 199 60 386 442
Cuts at: 3859 4205 5715 6190 7024 7538 8514 9199 10192
Size: 346 1510 475 834 514 976 685 993
Cuts at: 10192 10383 33
Size: 191 1121
Fragments arranged by size:
1635 1510 1121 993 976 834 685 514
500 475 442 386 361 346 243 199
191 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 1972 6436 1972
Size: 4464 7007
SfoI GGC'GCC
Cuts at: 245 825 6322 7602 7803 8313 8903 9151 245
Size: 580 5497 1280 201 510 590 248 2565
Fragments arranged by size:
5497 2565 1280 590 580 510 248 201
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 11471
SmaI CCC'GGG
Cuts at: 202 554 1843 3380 3406 3685 3876 4161 7555
Size: 352 1289 1537 26 279 191 285 3394
Cuts at: 7555 8493 202
Size: 938 3180
Fragments arranged by size:
3394 3180 1537 1289 938 352 285 279
191 26
SmlI C'TyrA_G
Cuts at: 7744 8233 8626 9541 10033 10295 7744
Size: 489 393 915 492 262 8920
Fragments arranged by size:
8920 915 492 489 393 262
SnaBI TAC'GTA
Cuts at: 8463 8463
Size: 11471
SpeI A'CTAG_T
Cuts at: 67 67
Size: 11471
SphI G_CATG'C
Cuts at: 2714 8524 9024 2714
Size: 5810 500 5161
Fragments arranged by size:
5810 5161 500
SrfI GCCC'GGGC
Cuts at: 202 202
Size: 11471
StuI AGG'CCT
Cuts at: 1124 1966 2055 2143 4814 1124
Size: 842 89 88 2671 7781
Fragments arranged by size:
7781 2671 842 89 88
StyI C'CwwG_G
Cuts at: 125 149 167 1304 1341 1459 1581 1866 1904
Size: 24 18 1137 37 118 122 285 38
Cuts at: 1904 2050 3294 3339 4466 5312 5610 6557 6755
Size: 146 1244 45 1127 846 298 947 198
Cuts at: 6755 6890 8018 8162 8605 8959 125
Size: 135 1128 144 443 354 2637
Fragments arranged by size:
2637 1244 1137 1128 1127 947 846 443
354 298 285 198 146 144 135 122
118 45 38 37 24 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 752 877 997 1546 1587 1942 2167 2229 2671
Size: 125 120 549 41 355 225 62 442
Cuts at: 2671 2854 3069 4312 4890 6930 8319 9054 9187
Size: 183 215 1243 578 2040 1389 735 133
Cuts at: 9187 9646 9855 752
Size: 459 209 2368
Fragments arranged by size:
2368 2040 1389 1243 735 578 549 459
442 355 225 215 209 183 133 125
120 62 41
TatI w'GTAC_w
Cuts at: 1817 2913 4541 6908 7494 8360 10986 1817
Size: 1096 1628 2367 586 866 2626 2302
Fragments arranged by size:
2626 2367 2302 1628 1096 866 586
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 349 381 432 464 1342 1374 1534 1566 1980
Size: 32 51 32 878 32 160 32 414
Cuts at: 1980 2012 2914 2946 5106 5138 5703 5735 5935
Size: 32 902 32 2160 32 565 32 200
Cuts at: 5935 5967 7036 7068 7258 7290 8174 8206 8261
Size: 32 1069 32 190 32 884 32 55
Cuts at: 8261 8293 10988 11020 349
Size: 32 2695 32 800
Fragments arranged by size:
2695 2160 1069 902 884 878 800 565
414 200 190 160 55 51 32 32
32 32 32 32 32 32 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 895 9268 895
Size: 8373 3098
XbaI T'CTAG_A
Cuts at: 8502 8502
Size: 11471
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4671 6361 7947 8966 4671
Size: 1690 1586 1019 7176
Fragments arranged by size:
7176 1690 1586 1019
XhoI C'TCGA_G
Cuts at: 8233 8233
Size: 11471
XmaI C'CCGG_G
Cuts at: 200 552 1841 3378 3404 3683 3874 4159 7553
Size: 352 1289 1537 26 279 191 285 3394
Cuts at: 7553 8491 200
Size: 938 3180
Fragments arranged by size:
3394 3180 1537 1289 938 352 285 279
191 26
XmnI GAAnn'nnTTC
Cuts at: 7214 7214
Size: 11471
ZraI GAC'GTC
Cuts at: 5704 5704
Size: 11471
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AflII AloI AscI BaeI BfrBI BmtI BsiWI
BssHII BstZ17I ClaI EcoRV FspAI HpaI MfeI NheI
NotI NruI NsiI PacI PsiI PspXI PsrI SgrAI
SspI SwaI