Restriction map for
pF1KSDA0792
(Circular) (Six-base) MAPSORT of: pF1KSDA0792.seq Check: 1901 from: 1 to: 5560
pF1KSDA0792 5560 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 984 2278 984
Size: 1294 4266
AatII G_ACGT'C
Cuts at: 299 299
Size: 5560
AccI GT'mk_AC
Cuts at: 1574 1643 2084 2596 1574
Size: 69 441 512 4538
Fragments arranged by size:
4538 512 441 69
Acc65I G'GTAC_C
Cuts at: 2574 2574
Size: 5560
AclI AA'CG_TT
Cuts at: 5400 5400
Size: 5560
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1234 3383 3815 4564 4832 1234
Size: 2149 432 749 268 1962
Fragments arranged by size:
2149 1962 749 432 268
AflIII A'CryG_T
Cuts at: 875 886 2910 4016 4993 875
Size: 11 2024 1106 977 1442
Fragments arranged by size:
2024 1442 1106 977 11
AgeI A'CCGG_T
Cuts at: 3948 3948
Size: 5560
AhdI GACnn_n'nnGTC
Cuts at: 393 393
Size: 5560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1855 1855
Size: 5560
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 124 156 1133 1165 124
Size: 32 977 32 4519
Fragments arranged by size:
4519 977 32 32
AlwNI CAG_nnn'CTG
Cuts at: 510 1164 1392 2863 4432 510
Size: 654 228 1471 1569 1638
Fragments arranged by size:
1638 1569 1471 654 228
ApaLI G'TGCA_C
Cuts at: 4330 4330
Size: 5560
ApoI r'AATT_y
Cuts at: 2562 4913 5169 2562
Size: 2351 256 2953
Fragments arranged by size:
2953 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5560
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5560
AvaI C'yCGr_G
Cuts at: 193 2578 193
Size: 2385 3175
BamHI G'GATC_C
Cuts at: 2111 2583 2111
Size: 472 5088
BanI G'GyrC_C
Cuts at: 761 1783 2458 2574 2988 3236 3271 761
Size: 1022 675 116 414 248 35 3050
Fragments arranged by size:
3050 1022 675 414 248 116 35
BanII G_rGCy'C
Cuts at: 1268 1295 2572 3602 1268
Size: 27 1277 1030 3226
Fragments arranged by size:
3226 1277 1030 27
BbeI G_GCGC'C
Cuts at: 2992 3240 2992
Size: 248 5312
BbsI GAAGACnn'nnnn_
Cuts at: 964 1006 1085 1758 1968 964
Size: 42 79 673 210 4556
Fragments arranged by size:
4556 673 210 79 42
BbvCI CC'TCA_GC
Cuts at: 2293 2293
Size: 5560
BciVI GTATCCnnnnn_n'
Cuts at: 890 3456 4219 5560 890
Size: 2566 763 1341 890
Fragments arranged by size:
2566 1341 890 763
BclI T'GATC_A
Cuts at: 1880 3078 1880
Size: 1198 4362
BfrBI ATG'CAT
Cuts at: 1960 1960
Size: 5560
BglI GCCn_nnn'nGGC
Cuts at: 1894 1894
Size: 5560
BglII A'GATC_T
Cuts at: 3073 5112 3073
Size: 2039 3521
BlpI GC'TnA_GC
Cuts at: 2670 2670
Size: 5560
Bme1580I G_kGCm'C
Cuts at: 3183 3276 4334 3183
Size: 93 1058 4409
Fragments arranged by size:
4409 1058 93
BmgBI CAC'GTC
Cuts at: 1149 1149
Size: 5560
BmrI ACTGGGnnnn_n'
Cuts at: 2770 2851 3186 5332 2770
Size: 81 335 2146 2998
Fragments arranged by size:
2998 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3925 3925
Size: 5560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 235 1141 1612 2122 166
Size: 69 906 471 510 3604
Fragments arranged by size:
3604 906 510 471 69
Bpu10I CC'TnA_GC
Cuts at: 2293 2293
Size: 5560
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2032 2734 3613 4107 4405 2032
Size: 702 879 494 298 3187
Fragments arranged by size:
3187 879 702 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1693 1861 5264 1693
Size: 168 3403 1989
Fragments arranged by size:
3403 1989 168
BsaAI yAC'GTr
Cuts at: 887 2382 2550 2911 887
Size: 1495 168 361 3536
Fragments arranged by size:
3536 1495 361 168
BsaBI GATnn'nnATC
Cuts at: 6 414 3072 5111 6
Size: 408 2658 2039 455
Fragments arranged by size:
2658 2039 455 408
BsaHI Gr'CG_yC
Cuts at: 296 530 1082 2989 3237 3941 5437 296
Size: 234 552 1907 248 704 1496 419
Fragments arranged by size:
1907 1496 704 552 419 248 234
BsaWI w'CCGG_w
Cuts at: 1712 2747 3268 3948 4222 4369 4780 1712
Size: 1035 521 680 274 147 411 2492
Fragments arranged by size:
2492 1035 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 417 805 417
Size: 388 5172
BseYI C'CCAG_C
Cuts at: 186 456 1062 1268 1392 2091 2983 3741 4320
Size: 270 606 206 124 699 892 758 579
Cuts at: 4320 4816 186
Size: 496 930
Fragments arranged by size:
930 892 758 699 606 579 496 270
206 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 694 925 694
Size: 231 5329
BsiEI CG_ry'CG
Cuts at: 104 3100 3146 4356 104
Size: 2996 46 1210 1308
Fragments arranged by size:
2996 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 251 1295 2572 3350 3540 4334 251
Size: 1044 1277 778 190 794 1477
Fragments arranged by size:
1477 1277 1044 794 778 190
Bsp1286I G_dGCh'C
Cuts at: 251 1268 1295 2572 3183 3276 3350 3540 3602
Size: 1017 27 1277 611 93 74 190 62
Cuts at: 3602 4334 251
Size: 732 1477
Fragments arranged by size:
1477 1277 1017 732 611 190 93 74
62 27
BspEI T'CCGG_A
Cuts at: 4780 4780
Size: 5560
BspHI T'CATG_A
Cuts at: 1628 4736 1628
Size: 3108 2452
BspMI ACCTGCnnnn'nnnn_
Cuts at: 984 998 2278 2608 2822 3124 3505 984
Size: 14 1280 330 214 302 381 3039
Fragments arranged by size:
3039 1280 381 330 302 214 14
BsrBI CCG'CTC
Cuts at: 50 3850 3904 5390 5555 50
Size: 3800 54 1486 165 55
Fragments arranged by size:
3800 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 365 1833 3470 365
Size: 1468 1637 2455
Fragments arranged by size:
2455 1637 1468
BsrFI r'CCGG_y
Cuts at: 200 2063 3556 3737 3948 200
Size: 1863 1493 181 211 1812
Fragments arranged by size:
1863 1812 1493 211 181
BssSI C'ACGA_G
Cuts at: 3829 4189 3829
Size: 360 5200
BstAPI GCAn_nnn'nTGC
Cuts at: 2935 2935
Size: 5560
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5560
BstEII G'GTnAC_C
Cuts at: 258 258
Size: 5560
BstXI CCAn_nnnn'nTGG
Cuts at: 1755 1755
Size: 5560
BstYI r'GATC_y
Cuts at: 1 506 2111 2583 3050 3073 3408 3654 4668
Size: 505 1605 472 467 23 335 246 1014
Cuts at: 4668 4754 4766 5112 1
Size: 86 12 346 449
Fragments arranged by size:
1605 1014 505 472 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 429 1334 429
Size: 905 4655
BtgI C'CryG_G
Cuts at: 1199 1460 2513 1199
Size: 261 1053 4246
Fragments arranged by size:
4246 1053 261
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1791 2001 3508 3689 3852 1791
Size: 210 1507 181 163 3499
Fragments arranged by size:
3499 1507 210 181 163
BtsI GCAGTG_nn'
Cuts at: 173 1585 2526 2801 173
Size: 1412 941 275 2932
Fragments arranged by size:
2932 1412 941 275
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1017 1052 1017
Size: 35 5525
DraI TTT'AAA
Cuts at: 2557 2557
Size: 5560
DraIII CAC_nnn'GTG
Cuts at: 2058 2058
Size: 5560
DrdI GACnn_nn'nnGTC
Cuts at: 2424 3264 4124 2424
Size: 840 860 3860
Fragments arranged by size:
3860 860 840
EaeI y'GGCC_r
Cuts at: 230 360 923 1038 1202 1886 3143 3317 3708
Size: 130 563 115 164 684 1257 174 391
Cuts at: 3708 3735 5082 230
Size: 27 1347 708
Fragments arranged by size:
1347 1257 708 684 563 391 174 164
130 115 27
EagI C'GGCC_G
Cuts at: 3143 3143
Size: 5560
EarI CTCTTCn'nnn_
Cuts at: 742 961 1417 2167 2312 3581 3791 742
Size: 219 456 750 145 1269 210 2511
Fragments arranged by size:
2511 1269 750 456 219 210 145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 822 3766 4078 4224 5370 822
Size: 2944 312 146 1146 1012
Fragments arranged by size:
2944 1146 1012 312 146
EcoICRI GAG'CTC
Cuts at: 1293 2570 1293
Size: 1277 4283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 235 1141 1234 1612 2122 3383 3815 4564
Size: 69 906 93 378 510 1261 432 749
Cuts at: 4564 4832 166
Size: 268 894
Fragments arranged by size:
1261 906 894 749 510 432 378 268
93 69
EcoNI CCTnn'n_nnAGG
Cuts at: 552 943 1749 2412 552
Size: 391 806 663 3700
Fragments arranged by size:
3700 806 663 391
EcoO109I rG'GnC_Cy
Cuts at: 1368 2205 2697 1368
Size: 837 492 4231
Fragments arranged by size:
4231 837 492
EcoRI G'AATT_C
Cuts at: 2562 2562
Size: 5560
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3577 3577
Size: 5560
FspI TGC'GCA
Cuts at: 3339 3339
Size: 5560
HaeII r_GCGC'y
Cuts at: 2513 2992 3240 4264 5243 2513
Size: 479 248 1024 979 2830
Fragments arranged by size:
2830 1024 979 479 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 379 411 1072 1104 3400 3430 3432 3462 379
Size: 32 661 32 2296 30 2 30 2477
Fragments arranged by size:
2477 2296 661 32 32 30 30 2
HincII GTy'rAC
Cuts at: 436 618 2597 436
Size: 182 1979 3399
Fragments arranged by size:
3399 1979 182
KasI G'GCGC_C
Cuts at: 2988 3236 2988
Size: 248 5312
KpnI G_GTAC'C
Cuts at: 2578 2578
Size: 5560
MluI A'CGCG_T
Cuts at: 875 4993 875
Size: 4118 1442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1736 4231 4415 1736
Size: 2495 184 2881
Fragments arranged by size:
2881 2495 184
MscI TGG'CCA
Cuts at: 925 1040 1204 1888 3319 925
Size: 115 164 684 1431 3166
Fragments arranged by size:
3166 1431 684 164 115
MslI CAynn'nnrTG
Cuts at: 1102 1882 3547 3674 1102
Size: 780 1665 127 2988
Fragments arranged by size:
2988 1665 780 127
MspA1I CmG'CkG
Cuts at: 186 302 909 1011 1376 2125 2477 2669 2983
Size: 116 607 102 365 749 352 192 314
Cuts at: 2983 3343 4358 4603 186
Size: 360 1015 245 1143
Fragments arranged by size:
1143 1015 749 607 365 360 352 314
245 192 116 102
NaeI GCC'GGC
Cuts at: 3739 3739
Size: 5560
NarI GG'CG_CC
Cuts at: 2989 3237 2989
Size: 248 5312
NcoI C'CATG_G
Cuts at: 1199 1460 1199
Size: 261 5299
NgoMIV G'CCGG_C
Cuts at: 3737 3737
Size: 5560
NruI TCG'CGA
Cuts at: 3921 3921
Size: 5560
NsiI A_TGCA'T
Cuts at: 1962 1962
Size: 5560
NspI r_CATG'y
Cuts at: 2361 2611 3111 4020 2361
Size: 250 500 909 3901
Fragments arranged by size:
3901 909 500 250
PasI CC'CwG_GG
Cuts at: 1164 1683 1164
Size: 519 5041
PciI A'CATG_T
Cuts at: 4016 4016
Size: 5560
PflMI CCAn_nnn'nTGG
Cuts at: 510 1164 2139 510
Size: 654 975 3931
Fragments arranged by size:
3931 975 654
PfoI T'CCnGG_A
Cuts at: 143 509 869 1151 1187 143
Size: 366 360 282 36 4516
Fragments arranged by size:
4516 366 360 282 36
PmeI GTTT'AAAC
Cuts at: 2557 2557
Size: 5560
PmlI CAC'GTG
Cuts at: 887 887
Size: 5560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4699 4731 4699
Size: 32 5528
PshAI GACnn'nnGTC
Cuts at: 1639 1639
Size: 5560
PsiI TTA'TAA
Cuts at: 337 337
Size: 5560
PstI C_TGCA'G
Cuts at: 995 2605 3290 995
Size: 1610 685 3265
Fragments arranged by size:
3265 1610 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5560
PvuII CAG'CTG
Cuts at: 186 302 909 2983 3343 186
Size: 116 607 2074 360 2403
Fragments arranged by size:
2403 2074 607 360 116
RsrII CG'GwC_CG
Cuts at: 3753 3753
Size: 5560
SacI G_AGCT'C
Cuts at: 1295 2572 1295
Size: 1277 4283
SalI G'TCGA_C
Cuts at: 2595 2595
Size: 5560
SapI GCTCTTCn'nnn_
Cuts at: 961 2312 3581 3791 961
Size: 1351 1269 210 2730
Fragments arranged by size:
2730 1351 1269 210
SbfI CC_TGCA'GG
Cuts at: 995 2605 995
Size: 1610 3950
ScaI AGT'ACT
Cuts at: 5077 5077
Size: 5560
SexAI A'CCwGG_T
Cuts at: 797 797
Size: 5560
SfcI C'TryA_G
Cuts at: 33 682 991 1384 1995 2601 3286 4281 4472
Size: 649 309 393 611 606 685 995 191
Cuts at: 4472 33
Size: 1121
Fragments arranged by size:
1121 995 685 649 611 606 393 309
191
SfoI GGC'GCC
Cuts at: 2990 3238 2990
Size: 248 5312
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5560
SmaI CCC'GGG
Cuts at: 2580 2580
Size: 5560
SmlI C'TyrA_G
Cuts at: 2047 2713 3628 4122 4384 2047
Size: 666 915 494 262 3223
Fragments arranged by size:
3223 915 666 494 262
SnaBI TAC'GTA
Cuts at: 2550 2550
Size: 5560
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5560
SphI G_CATG'C
Cuts at: 2611 3111 2611
Size: 500 5060
StuI AGG'CCT
Cuts at: 1744 1930 2137 2545 1744
Size: 186 207 408 4759
Fragments arranged by size:
4759 408 207 186
StyI C'CwwG_G
Cuts at: 161 1199 1460 2138 2692 3046 161
Size: 1038 261 678 554 354 2675
Fragments arranged by size:
2675 1038 678 554 354 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3141 3274 3733 3944 3141
Size: 133 459 211 4757
Fragments arranged by size:
4757 459 211 133
TatI w'GTAC_w
Cuts at: 782 5075 782
Size: 4293 1267
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 529 561 757 789 1956 1988 5077 5109 529
Size: 32 196 32 1167 32 3089 32 980
Fragments arranged by size:
3089 1167 980 196 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1608 3355 1608
Size: 1747 3813
XbaI T'CTAG_A
Cuts at: 1698 2589 1698
Size: 891 4669
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 891 1942 2520 3053 891
Size: 1051 578 533 3398
Fragments arranged by size:
3398 1051 578 533
XmaI C'CCGG_G
Cuts at: 2578 2578
Size: 5560
XmnI GAAnn'nnTTC
Cuts at: 1222 1222
Size: 5560
ZraI GAC'GTC
Cuts at: 297 297
Size: 5560
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AleI ApaI AscI AvrII BaeI BcgI
BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
ClaI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI PacI PpuMI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SrfI SspI SwaI XhoI